Updated on 2024/04/24

写真a

 
SHIOTA Masayuki
 
Organization
Graduate School of Medicine Department of Basic Medical Science Associate Professor
School of Medicine Department of Medical Science
Title
Associate Professor
Affiliation
Institute of Medicine
Contact information
メールアドレス
Affiliation campus
Abeno Campus

Position

  • Graduate School of Medicine Department of Basic Medical Science 

    Associate Professor  2022.04 - Now

  • School of Medicine Department of Medical Science 

    Associate Professor  2022.04 - Now

Degree

  • 博士(農学) ( University of Tsukuba )

Research Areas

  • Life Science / Molecular biology

  • Life Science / Pathological biochemistry

Research Interests

  • Proteostasis

  • Heat Shock Protein

  • Cancer

  • Proteomics

  • antibody therapeutic

Research subject summary

  • プロテオスタシス(タンパク質の恒常性維持)は、細胞の生存にとって極めて重要であり、プロテオスタシスの破綻は疾患と深くリンクしている。特にがん細胞は、急速な増殖のために慢性的なストレス状態にあり、その生存はプロテオスタシスの亢進に強く依存している。このプロテオスタシスの制御機構の中心に位置するのが、分子シャペロンである一群の熱ショックタンパク質(HSP)である。 HSPは、タンパク質の適切なフォールディングの触媒、アポトーシス機構の調節、およびストレス条件下での変性タンパク質の修復または分解の選択と、タンパク質分解機構の調節に関わることで、細胞の生存を図っている。そこで、HSPの中でも細胞保護効果の最も強いHSP70に焦点をあて、HSP70が結合することで新生・修復・分解するタンパク質群を包括的に明らかにし、がんの病態や薬剤の治療抵抗性の機序の解明に取り組んでいる。

  • 抗体医薬品としての臨床応用を目指した治療効果を有するモノクローナル抗体の作製を進めている。特に抗腫瘍活性を有する新規抗癌薬の創製を進めている。

Research Career

  • 抗腫瘍活性を有する抗体の創製

    2022.04 - Now 

  • proteostasis and disease

    heat shock protein, stress, proteomics  Joint Research in Japan

    2010.04 - Now 

Professional Memberships

  • 日本分子生物学会

      Domestic

  • 日本薬理学会

      Domestic

  • 日本癌学会

      Domestic

  • 日本臨床ストレス応答学会

      Domestic

Papers

  • Characterizing the toxicological responses to inorganic arsenicals and their metabolites in immortalized human bladder epithelial cells. Reviewed

    Vachiraarunwong A, Gi M, Kiyono T, Suzuki S, Fujioka M, Qiu G, Guo R, Yamamoto T, Kakehashi A, Shiota M, Wanibuchi H

    Archives of toxicology   2024.04( ISSN:0340-5761

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s00204-024-03750-1

    PubMed

  • OTUD1 deubiquitinase regulates NF-κB- and KEAP1-mediated inflammatory responses and reactive oxygen species-associated cell death pathways. Reviewed

    Oikawa D, Gi M, Kosako H, Shimizu K, Takahashi H, Shiota M, Hosomi S, Komakura K, Wanibuchi H, Tsuruta D, Sawasaki T, Tokunaga F

    Cell death & disease   13 ( 8 )   694   2022.08

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41419-022-05145-5

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  • The carbonic anhydrase inhibitor acetazolamide inhibits urinary bladder cancers via suppression of β-catenin signaling. Reviewed

    Matsue T, Gi M, Shiota M, Tachibana H, Suzuki S, Fujioka M, Kakehashi A, Yamamoto T, Kato M, Uchida J, Wanibuchi H

    Cancer science   113 ( 8 )   2642 - 2653   2022.08( ISSN:1347-9032

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/cas.15467

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  • Suppression of the doxorubicin response by hypoxia-inducible factor-1α is strictly dependent on oxygen concentrations under hypoxic conditions. Reviewed

    Mayuko Osada-Oka, Hikaru Kuwamura, Risa Imamiya, Keiko Kobayashi, Yukiko Minamiyama, Katsuyuki Takahashi, Masako Tanaka, Masayuki Shiota

    European journal of pharmacology   920   174845 - 174845   2022.04( ISSN:0014-2999

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Hypoxia-inducible factor-1α (HIF-1α) and p53 are involved in anticancer drug resistance under hypoxic conditions. Here, we found that the cytotoxicity of anticancer drugs (doxorubicin, gemcitabine, and cisplatin) was lower at 1% O2 than at 5% O2. We examined the effects of these drugs on HIF-1α and p53 expression under different hypoxic oxygen concentrations. At 5% O2, the drugs decreased HIF-1α expression and increased p53 levels. At 1% O2, the drugs increased HIF-1α expression but did not alter p53 levels. When the HIF-1α protein was stabilized by DMOG under normoxic conditions, doxorubicin did not increase the level of p53 expression. These results show that the maintenance of HIF-1α expression blocked doxorubicin-dependent increases in p53 expression. We hypothesized the mechanism of HIF-1α protein translation might be different between at 5% and at 1% O2, because many reports indicate that the same mechanism of HIF-1α protein stabilization occurs under hypoxic conditions, such as 5% and 1% O2. The level of phosphorylated-4E-BP1, which causes translation of HIF-1α, was higher at 1% O2 than at 5% O2. Our results suggest that the sensitivity of tumor cells to anticancer drugs is dependent oxygen concentrations under hypoxic conditions, and involves 4E-BP1-dependent stabilization of the HIF-1α protein.

    DOI: 10.1016/j.ejphar.2022.174845

    PubMed

  • ER membrane protein complex 1 interacts with STIM1 and regulates store-operated Ca2+ entry. Reviewed

    Kazuhiko Kawata, Akemi Baba, Masayuki Shiota, Hideki Wanibuchi, Yoshihiro Baba

    Journal of biochemistry   170 ( 4 )   483 - 488   2021.12( ISSN:0021-924X

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Store-operated calcium entry (SOCE) is the process by which the emptying of endoplasmic reticulum (ER) Ca2+ stores causes an influx of Ca2+ across the plasma membrane (PM). It is the major Ca2+ influx pathway in nonexcitable cells and has a wide array of physiological functions. Upon store depletion, stromal interaction molecule 1 (STIM1), an ER calcium sensor relocates into discrete puncta at the ER-PM junction region, which results in the coupling of Ca2+ channels to initiate SOCE. However, the mechanism regulating STIM1 activity remains poorly understood. Here, we performed affinity purification of STIM1 and uncovered ER membrane protein complex 1 (EMC1) as an STIM1 binding partner. We showed that this interaction occurred in the ER through the intraluminal region of STIM1. After store depletion, EMC1 does not cluster adjacent to the PM, which suggests that it is distributed differently from STIM1. EMC1 knockdown with small interfering RNA resulted in a marked decrease in SOCE. Thus, these findings suggest that EMC1 functions as a positive regulator of SOCE.

    DOI: 10.1093/jb/mvab063

    PubMed

  • Canopy Homolog 2 as a Novel Molecular Target in Hepatocarcinogenesis. Reviewed

    Anna Kakehashi, Shugo Suzuki, Masayuki Shiota, Nina Raymo, Min Gi, Taro Tachibana, Vasily Stefanov, Hideki Wanibuchi

    Cancers   13 ( 14 )   2021.07( ISSN:2072-6694

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    In the present study, the role of a novel protein involved in neurite development and endoplasmic reticulum (ER) stress, canopy homolog 2 (CNPY2), was investigated in mouse and human hepatocarcinogenesis. Firstly, a sensitive quantitative and qualitative detection of protein expression using QSTAR Elite LC-Ms/Ms was performed for the analysis of lysates of microdissected hepatocellular altered foci (AF), adenomas (HCAs), carcinomas (HCCs) and peri-tumoral livers from C57Bl/6J mice treated with diethylnitrosamine (DEN) and then maintained for 27 or 38 weeks on basal diet. Significant overexpression of 18.5 kDa CNPY2 processed form was demonstrated in AF, HCAs and HCCs, while low expression was observed in the livers of DEN-treated and control mice. Furthermore, CNPY2 elevation in AF and tumors was coordinated with accumulation of numerous cytoskeletal proteins, including cytokeratins 8 and 18, actin, non-muscle myosin and septin 9 and those involved in ER and mitochondrial stresses such as calreticulin, prohibitins 1 and 2 and YME1-like-1. Knockdown of CNPY2 in Huh7 and HepG2 human liver cancer cells resulted in significant suppression of cell survival and invasive potential, inhibition of cyclin D1, induction of p21Waf1/Cip1 and suppression of the apoptosis inhibitor Bcl2. In contrast, transfection of a mouse CNPY2 (mCNPY2-Ds-Red) vector plasmid in Huh7 and HepG2 cancer cells, with subsequent accumulation of CNPY2 in the ER, resulted in significant increase in cancer cells survival. Clinicopathological analysis in 90 HCV-positive HCC patients, revealed significant association of CNPY2 overexpression with poor overall (p = 0.041) survival. Furthermore, CNPY2 increase was associated with vessel invasion (p = 0.038), poor histological differentiation (p = 0.035) and advanced clinical stage (p = 0.016). In conclusion, CNPY2 is a promising molecular target elevated early in hepatocarcinogenesis and prognostic marker for human HCV-associated HCC. CNPY2 is involved in the processes of ER stress, cell cycle progression, proliferation, survival and invasion of liver tumor cells.

    DOI: 10.3390/cancers13143613

    PubMed

  • Upregulation of PTK7 and β-catenin after vaginal mechanical dilatation: an examination of fibulin-5 knockout mice. Reviewed

    Uemura R, Tachibana D, Shiota M, Yoshida K, Kitada K, Hamuro A, Misugi T, Koyama M

    International urogynecology journal   2021.02( ISSN:0937-3462

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    DOI: 10.1007/s00192-021-04693-2

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  • Effects of orally active hypoxia inducible factor alpha prolyl hydroxylase inhibitor, FG4592 on renal fibrogenic potential in mouse unilateral ureteral obstruction model Reviewed

    Kabei Kazuya, Tateishi Yu, Shiota Masayuki, Osada-Oka Mayuko, Nishide Shunji, Uchida Junji, Nakatani Tatsuya, Matsunaga Shinji, Yamaguchi Takehiro, Tomita Shuhei, Miura Katsuyuki

    JOURNAL OF PHARMACOLOGICAL SCIENCES   142 ( 3 )   93 - 100   2020.03( ISSN:1347-8613

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jphs.2019.12.002

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  • The Autophagy-Related Protein GABARAP Is Induced during Overwintering in the Bean Bug (Hemiptera: Alydidae) Reviewed

    Tachibana Shin-Ichiro, Matsuzaki Shinji, Tanaka Masako, Shiota Masayuki, Motooka Daisuke, Nakamura Shota, Goto Shin G.

    JOURNAL OF ECONOMIC ENTOMOLOGY   113 ( 1 )   427 - 434   2020.02( ISSN:0022-0493

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/jee/toz287

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  • Generation of Rat Monoclonal Antibodies Specific for Human Stromal Cell-Derived Factor-2 Reviewed

    Tanaka M, Shiota M, Koyama M, Nakayama J, Yashiro M, Semba K, Goda N

    Monoclon Antib Immunodiagn Immunother   39 ( 1 )   23 - 26   2020.01

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  • Effects of orally active hypoxia inducible factor alpha prolyl hydroxylase inhibitor, FG4592 on renal fibrogenic potential in mouse unilateral ureteral obstruction model. Reviewed

    Kabei K, Tateishi Y, Shiota M, Osada-Oka M, Nishide S, Uchida J, Nakatani T, Matsunaga S, Yamaguchi T, Tomita S, Miura K.

    J Pharmacol Sci.   142 ( 3 )   93 - 100   2019.12

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • The Autophagy-Related Protein GABARAP Is Induced during Overwintering in the Bean Bug (Hemiptera: Alydidae). Reviewed

    Tachibana SI, Matsuzaki S, Tanaka M, Shiota M, Motooka D, Nakamura S, Goto SG

    Journal of Economic Entomology   pii: toz287.   2019.11

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  • Controlling the Phenotype of Tumor-Infiltrating Macrophages via the PHD-HIF Axis Inhibits Tumor Growth in a Mouse Model. Reviewed

    Nishide S, Matsunaga S, Shiota M, Yamaguchi T, Kitajima S, Maekawa Y, Takeda N, Tomura M, Uchida J, Miura K, Nakatani T, Tomita S

    iScience.   19   940 - 954   2019.08

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  • Therapeutic Silencing of Centromere Protein X Ameliorates Hyperglycemia in Zebrafish and Mouse Models of Type 2 Diabetes Mellitus. Reviewed

    Zang L, Shimada Y, Nakayama H, Chen W, Okamoto A, Koide H, Oku N, Dewa T, Shiota M, Nishimura N.

    Frontiers in Genetics   10   693   2019.07

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  • A dipeptidyl peptidase-4 (DPP-4) inhibitor, linagliptin, attenuates cardiac dysfunction after myocardial infarction independently of DPP-4. Reviewed

    Yamaguchi T, Watanabe A, Tanaka M, Shiota M, Osada-Oka M, Sano S, Yoshiyama M, Miura K, Kitajima S, Matsunaga S, Tomita S, Iwao H, Izumi Y.

    J Pharmacol Sci.   132 ( 2 )   112 - 119   2018.12

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  • Generation of Rat Monoclonal Antibodies Against a Deubiquitinase, Ovarian Tumor Domain-Containing Protein 1. Reviewed

    Oikawa D, Shiota M, Goto E, Komakura K, Wanibuchi H, Tokunaga F

    Monoclonal antibodies in immunodiagnosis and immunotherapy   37 ( 4 )   180 - 184   2018.08

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1089/mab.2018.0023

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  • Generation of Rat Monoclonal Antibodies Specific for DZIP3. Reviewed

    Oikawa D, Shiota M, Tokunaga F, Wanibuchi H

    Monoclonal antibodies in immunodiagnosis and immunotherapy   37 ( 3 )   153 - 157   2018.06

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1089/mab.2018.0005

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  • Role of hypoxia-inducible factor-1 in the development of renal fibrosis in mouse obstructed kidney: Special references to HIF-1 dependent gene expression of profibrogenic molecules. Reviewed

    Kabei K, Tateishi Y, Nozaki M, Tanaka M, Shiota M, Osada-Oka M, Nishide S, Uchida J, Nakatani T, Tomita S, Miura K

    Journal of pharmacological sciences   136   31 - 38   2017.12( ISSN:1347-8613

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1016/j.jphs.2017.12.004

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  • Heat shock protein 70 inhibitors suppress androgen receptor expression in LNCaP95 prostate cancer cells Reviewed

    Kita Kazuaki, Shiota Masayuki, Tanaka Masako, Otsuka Asuka, Matsumoto Masaki, Kato Minoru, Tamada Satoshi, Iwao Hiroshi, Miura Katsuyuki, Nakatani Tatsuya, Tomita Shuhei

    CANCER SCIENCE   108 ( 9 )   1820 - 1827   2017.09( ISSN:1349-7006

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    DOI: 10.1111/cas.13318

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  • Macrophage-derived exosomes induce inflammatory factors in endothelial cells under hypertensive conditions Reviewed

    Osada-Oka Mayuko, Shiota Masayuki, Izumi Yasukatsu, Nishiyama Masaki, Tanaka Masako, Yamaguchi Takehiro, Sakurai Emi, Miura Katsuyuki, Iwao Hiroshi

    HYPERTENSION RESEARCH   40 ( 4 )   353 - 360   2017.04( ISSN:0916-9636

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    DOI: 10.1038/hr.2016.163

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  • Protection of stromal cell-derived factor 2 by heat shock protein 72 prevents oxaliplatin-induced cell death in oxaliplatin-resistant human gastric cancer cells Reviewed

    Takahashi Katsuyuki, Tanaka Masako, Yashiro Masakazu, Matsumoto Masaki, Ohtsuka Asuka, Nakayama Keiichi I., Izumi Yasukatsu, Nagayama Katsuya, Miura Katsuyuki, Iwao Hiroshi, Shiota Masayuki

    CANCER LETTERS   378 ( 1 )   8 - 15   2016.08( ISSN:0304-3835

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    DOI: 10.1016/j.canlet.2016.05.002

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  • Identification of low-abundance proteins in serum via the isolation of HSP72 complexes Reviewed

    Tanaka Masako, Shiota Masayuki, Nakao Takafumi, Uemura Ryo, Nishi Satoshi, Ohkawa Yasuyuki, Matsumoto Masaki, Yamaguchi Maki, Osada-Oka Mayuko, Inagaki Azusa, Takahashi Katsuyuki, Nakayama Keiichi I., Gi Min, Izumi Yasukatsu, Miura Katsuyuki, Iwao Hiroshi

    JOURNAL OF PROTEOMICS   136   214 - 221   2016.05( ISSN:1874-3919

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    DOI: 10.1016/j.jprot.2016.01.008

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  • Percutaneous Carbon Dioxide Treatment Using a Gas Mist Generator Attenuates the Development of Right Ventricular Dysfunction in Monocrotaline-induced Pulmonary Hypertensive Rats. Reviewed

    Yamaguchi T, Izumi Y, Yamazaki T, Nakamura Y, Sano S, Shiota M, Miura K, Iwao H, Yoshiyama M

    Osaka city medical journal   61 ( 1 )   31 - 41   2015.06( ISSN:0030-6096

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  • Percutaneous carbon dioxide mist treatment has protective effects in experimental myocardial infarction Reviewed

    Yamaguchi Takehiro, Yamazaki Takanori, Nakamura Yasuhiro, Shiota Masayuki, Shimada Kenei, Miura Katsuyuki, Iwao Hiroshi, Yoshiyama Minoru, Izumi Yasukatsu

    JOURNAL OF PHARMACOLOGICAL SCIENCES   127 ( 4 )   474 - 480   2015.04( ISSN:1347-8613

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1016/j.jphs.2015.03.009

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  • Myeloid HIF-1 attenuates the progression of renal fibrosis in murine obstructive nephropathy Reviewed

    Tateishi Yu, Osada-Oka Mayuko, Tanaka Masako, Shiota Masayuki, Izumi Yasukatsu, Ishimura Eiji, Motoyama Koka, Inaba Masaaki, Miura Katsuyuki

    JOURNAL OF PHARMACOLOGICAL SCIENCES   127 ( 2 )   181 - 189   2015.02( ISSN:1347-8613

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1016/j.jphs.2014.12.011

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  • Repeated remote ischemic conditioning attenuates left ventricular remodeling via exosome-mediated intercellular communication on chronic heart failure after myocardial infarction Reviewed

    Yamaguchi Takehiro, Izumi Yasukatsu, Nakamura Yasuhiro, Yamazaki Takanori, Shiota Masayuki, Sano Soichi, Tanaka Masako, Osada-Oka Mayuko, Shimada Kenei, Miura Katuyuki, Yoshiyama Minoru, Iwao Hiroshi

    INTERNATIONAL JOURNAL OF CARDIOLOGY   178   239 - 246   2015.01( ISSN:0167-5273

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1016/j.ijcard.2014.10.144

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  • Percutaneous Carbon Dioxide Treatment using a Gas Mist Generator Enhances the Collateral Blood Flow in the Ischemic Hindlimb Reviewed

    Izumi Yasukatsu, Yamaguchi Takehiro, Yamazaki Takanori, Yamashita Naoto, Nakamura Yasuhiro, Shiota Masayuki, Tanaka Masako, Sano Soichi, Osada-Oka Mayuko, Shimada Kenei, Wanibuchi Hideki, Miura Katsuyuki, Yoshiyama Minoru, Iwao Hiroshi

    Japan Atherosclerosis Society Journal of Atherosclerosis and Thrombosis   22 ( 1 )   38 - 51   2015( ISSN:1340-3478

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.5551/jat.23770

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  • A Noninvasive Metabolic Syndrome Model Using an Extremely Small Minipig, the Microminipig Reviewed

    Yamaguchi Takehiro, Yamazaki Takanori, Kawaguchi Hiroaki, Tawa Masashi, Nakamura Yasuhiro, Shiota Masayuki, Osada-Oka Mayuko, Tanimoto Akihide, Okamura Tomio, Miura Katsuyuki, Iwao Hiroshi, Yoshiyama Minoru, Izumi Yasukatsu

    The Japanese Pharmacological Society JOURNAL OF PHARMACOLOGICAL SCIENCES   126 ( 2 )   168 - 171   2014.10( ISSN:1347-8613

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1254/jphs.14171SC

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  • Establishment of neutralizing rat monoclonal antibodies for fibroblast growth factor-2. Reviewed

    Tanaka M, Yamaguchi M, Shiota M, Kawamoto Y, Takahashi K, Inagaki A, Osada-Oka M, Harada A, Wanibuchi H, Izumi Y, Miura K, Iwao H, Ohkawa Y

    Monoclonal antibodies in immunodiagnosis and immunotherapy   33 ( 4 )   261 - 9   2014.08

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    DOI: 10.1089/mab.2013.0085

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  • Hsc70 Contributes to Cancer Cell Survival by Preventing Rab1A Degradation under Stress Conditions Reviewed

    Tanaka Masako, Mun Saya, Harada Akihito, Ohkawa Yasuyuki, Inagaki Azusa, Sano Soichi, Takahashi Katsuyuki, Izumi Yasukatsu, Osada-Oka Mayuko, Wanibuchi Hideki, Yamagata Masayo, Yukimura Tokihito, Miura Katsuyuki, Shiota Masayuki, Iwao Hiroshi

    PLOS ONE   9 ( 5 )   e96785   2014.05( ISSN:1932-6203

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    DOI: 10.1371/journal.pone.0096785

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  • Lipid synthesis is promoted by hypoxic adipocyte-derived exosomes in 3T3-L1 cells Reviewed

    Sano Soichi, Izumi Yasukatsu, Yamaguchi Takehiro, Yamazaki Takanori, Tanaka Masako, Shiota Masayuki, Osada-Oka Mayuko, Nakamura Yasuhiro, Wei Min, Wanibuchi Hideki, Iwao Hiroshi, Yoshiyama Minoru

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   445 ( 2 )   327 - 333   2014.03( ISSN:0006-291X

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    DOI: 10.1016/j.bbrc.2014.01.183

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  • A novel feature of the HSP70 family Reviewed

    Shiota Masayuki, Tanaka Masako

    The Japanese Pharmacological Society, Folia Pharmacologica Japonica   143 ( 6 )   310 - 312   2014( ISSN:0015-5691

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    DOI: 10.1254/fpj.143.310

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  • Establishment of neutralizing rat monoclonal antibodies for fibroblast growth factor-2 Reviewed

    Monoclonal Antibodies Immunodiagn. Immunother.   33   261 - 269   2014

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  • Establishment of a 5-fluorouracil-resistant triple-negative breast cancer cell line Reviewed

    Takahashi Katsuyuki, Tanaka Masako, Inagaki Azusa, Wanibuchi Hideki, Izumi Yasukatsu, Miura Katsuyuki, Nagayama Katsuya, Shiota Masayuki, Iwao Hiroshi

    INTERNATIONAL JOURNAL OF ONCOLOGY   43 ( 6 )   1985 - 1991   2013.12( ISSN:1019-6439

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    DOI: 10.3892/ijo.2013.2135

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  • Tolvaptan Attenuates Left Ventricular Fibrosis Afer Acute Myocardial Infarction in Rats Reviewed

    Yamazaki Takanori, Nakamura Yasuhiro, Shiota Masayuki, Osada-Oka Mayuko, Fujiki Hiroyuki, Hanataani Akihisa, Shimada kenei, Miura Katsuyuki, Yoshiyama Minoru, Iwao Hiroshi, Izumi Yasukatsu

    The Japanese Pharmacological Society, The Japanese Journal of Pharmacology   123 ( 1 )   58 - 66   2013.09( ISSN:1347-8613

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1254/jphs.13086FP

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  • Tolvaptan Improves Left Ventricular Dysfunction after Myocardial Infarction in Rats Reviewed

    Yamazaki Takanori, Izumi Yasukatsu, Nakamura Yasuhiro, Yamashita Naoto, Fujiki Hiroyuki, Osada-Oka Mayuko, Shiota Masayuki, Hanatani Akihisa, Shimada Kenei, Iwao Hiroshi, Yoshiyama Minoru

    CIRCULATION-HEART FAILURE   5 ( 6 )   794 - 802   2012.11( ISSN:1941-3289

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    DOI: 10.1161/CIRCHEARTFAILURE.112.968750

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  • Pravastatin-induced proangiogenic effects depend upon extracellular FGF-2 Reviewed

    Shiota Masayuki, Hikita Yuko, Kawamoto Yukiko, Kusakabe Hiromi, Tanaka Masako, Izumi Yasukatsu, Nakao Takafumi, Miura Katsuyuki, Funae Yoshihiko, Iwao Hiroshi

    JOURNAL OF CELLULAR AND MOLECULAR MEDICINE   16 ( 9 )   2001 - 2009   2012.09( ISSN:1582-1838

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1111/j.1582-4934.2011.01494.x

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  • Surf4 modulates STIM1-dependent calcium entry Reviewed

    Fujii Yoko, Shiota Masayuki, Ohkawa Yasuyuki, Baba Akemi, Wanibuchi Hideki, Kinashi Tatsuo, Kurosaki Tomohiro, Baba Yoshihiro

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   422 ( 4 )   615 - 620   2012.06( ISSN:0006-291X

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    DOI: 10.1016/j.bbrc.2012.05.037

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  • Dexmedetomidine infusion for sedation in the intensive care setting in an infant with airway compromise due to congenital mediastinal neuroblastoma Reviewed

    Shiota Mitsutaka, Oda Yutaka, Taniguchi Masashi, Hamabata Takayuki, Mizumoto Hiroshi, Hata Daisuke

    PEDIATRIC ANESTHESIA   22 ( 6 )   603 - 605   2012.06( ISSN:1155-5645

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/j.1460-9592.2012.03865.x

  • Novel Device That Produces Carbon Dioxide Mist for Myocardial Infarction Treatment in Rats Reviewed

    Yamazaki Takanori, Izumi Yasukatsu, Nakamura Yasuhiro, Hanatani Akihisa, Shimada Kenei, Muro Takashi, Shiota Masayuki, Iwao Hiroshi, Yoshiyama Minoru

    The Japanese Circulation Society CIRCULATION JOURNAL   76 ( 5 )   1203 - 1212   2012.05( ISSN:1346-9843

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    DOI: 10.1253/circj.CJ-11-1116

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  • Red Ginseng Inhibits Scratching Behavior Associated With Atopic Dermatitis in Experimental Animal Models Reviewed

    Samukawa Keiichi, Izumi Yasukatsu, Shiota Masayuki, Nakao Takafumi, Osada-Oka Mayuko, Miura Katsuyuki, Iwao Hiroshi

    The Japanese Pharmacological Society JOURNAL OF PHARMACOLOGICAL SCIENCES   118 ( 3 )   391 - 400   2012.03( ISSN:1347-8613

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    DOI: 10.1254/jphs.11182FP

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  • The antifibrotic agent pirfenidone inhibits angiotensin II-induced cardiac hypertrophy in mice Reviewed

    YAMAZAKI Takanori, YAMASHITA Naoto, IZUMI Yasukatsu, NAKAMURA Yasuhiro, SHIOTA Masayuki, HANATANI Akihisa, SHIMADA Kenei, MURO Takashi, IWAO Hiroshi, YOSHIYAMA Minoru

    Hypertension research : clinical and experimental : official journal of the Japanese Society of Hypertension   35 ( 1 )   34 - 40   2012.01( ISSN:09169636

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  • The antifibrotic agent pirfenidone inhibits angiotensin II-induced cardiac hypertrophy in mice Reviewed

    Yamazaki Takanori, Yamashita Naoto, Izumi Yasukatsu, Nakamura Yasuhiro, Shiota Masayuki, Hanatani Akihisa, Shimada Kenei, Muro Takashi, Iwao Hiroshi, Yoshiyama Minoru

    HYPERTENSION RESEARCH   35 ( 1 )   34 - 40   2012.01( ISSN:0916-9636

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    DOI: 10.1038/hr.2011.139

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  • Generation of a Rat Monoclonal Antibody Specific for Hsp72 Reviewed

    Tanaka Masako, Shiota Masayuki, Okada Seiji, Harada Akihito, Odawara Jun, Mun Saya, Iwao Hiroshi, Ohkawa Yasuyuki

    HYBRIDOMA   30 ( 4 )   397 - 400   2011.08( ISSN:1554-0014

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    DOI: 10.1089/hyb.2011.0015

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  • Severe prekallikrein deficiency due to a homozygous Trp499Stop nonsense mutation Reviewed

    Nakao Takafumi, Yamane Takahisa, Katagami Tomoko, Shiota Masayuki, Izumi Yasukatsu, Samori Tomohiro, Hino Masayuki, Iwao Hiroshi

    BLOOD COAGULATION & FIBRINOLYSIS   22 ( 4 )   337 - 339   2011.06( ISSN:0957-5235

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    DOI: 10.1097/MBC.0b013e3283444ddb

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  • Renal Fibrosis in Murine Obstructive Nephropathy Is Attenuated by Depletion of Monocyte Lineage, Not Dendritic Cells Reviewed

    Machida Yuichi, Kitamoto Koichiro, Izumi Yasukatsu, Shiota Masayuki, Uchida Junji, Kira Yukimi, Nakatani Tatsuya, Miura Katsuyuki

    The Japanese Pharmacological Society JOURNAL OF PHARMACOLOGICAL SCIENCES   114 ( 4 )   464 - 473   2010.12( ISSN:1347-8613

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    DOI: 10.1254/jphs.10246FP

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  • Generation of a Rat Monoclonal Antibody Specific for Heat Shock Cognate Protein 70 Reviewed

    Shiota Masayuki, Saiwai Hirokazu, Mun Saya, Harada Akihito, Okada Seiji, Odawara Jun, Tanaka Masako, Iwao Hiroshi, Ohkawa Yasuyuki

    HYBRIDOMA   29 ( 5 )   453 - 456   2010.10( ISSN:1554-0014

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    DOI: 10.1089/hyb.2010.0024

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  • Heat Shock Cognate Protein 70 is essential for Akt signaling in Endothelial Function Reviewed

    SHIOTA Masayuki, IZUMI Yasukatsu, NAKAO Takafumi, IWAO Hiroshi

    33 ( 2 )   49 - 54   2010.07( ISSN:09114637

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  • 内皮機能におけるHeat shock cognate protein 70(Hsc70)の役割 Reviewed

    塩田 正之, 泉 康雄, 中尾 隆文, 岩尾 洋

    日本心脈管作動物質学会 血管   33 ( 2 )   49 - 54   2010.07( ISSN:0911-4637

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    内皮機能におけるHeat shock cognate protein 70(Hsc70)の役割について検討した。臍帯静脈内皮細胞(HUVEC)は、継代数3から5までのものを実験に供した。8週齢雄性C57BL/6Jマウスの左大腿動静脈を摘出することで下肢虚血モデルを作成した。Hsc70の機能阻害がAktを不活性化することが明らかになった。Hsc70をノックダウンした細胞抽出液を用いてAkt経路に関わる分子のリン酸化、総タンパク量の変化を検討し、PI3Kp110サブユニットのタンパク質発現が優位に低下していた。p85サブユニットは発現低下傾向を認めるものの有意差はなかった。KNK437処置の場合でも同様にPI3Kp110の有意な発現低下を認めた。Hsc70はPI3Kp110のmRNAレベルでの制御に寄与することで、内皮機能を正常に維持し、血管新生を制御していることが明らかとなった。

  • Heat Shock Cognate Protein 70 Is Essential for Akt Signaling in Endothelial Function Reviewed

    Shiota Masayuki, Kusakabe Hiromi, Izumi Yasukatsu, Hikita Yuko, Nakao Takafumi, Funae Yoshihiko, Miura Katsuyuki, Iwao Hiroshi

    ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY   30 ( 3 )   491 - U279   2010.03( ISSN:1079-5642

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    DOI: 10.1161/ATVBAHA.109.193631

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  • Effects of Liposome Clodronate on Renal Leukocyte Populations and Renal Fibrosis in Murine Obstructive Nephropathy Reviewed

    Kitamoto Koichiro, Machida Yuichi, Uchida Junji, Izumi Yasukatsu, Shiota Masayuki, Nakao Takafumi, Iwao Hiroshi, Yukimura Tokihito, Nakatani Tatsuya, Miura Katsuyuki

    The Japanese Pharmacological Society JOURNAL OF PHARMACOLOGICAL SCIENCES   111 ( 3 )   285 - 292   2009.11( ISSN:1347-8613

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    DOI: 10.1254/jphs.09227FP

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  • Pravastatin accelerates ischemia-induced angiogenesis through AMP-activated protein kinase Reviewed

    Izumi Yasukatsu, Shiota Masayuki, Kusakabe Hiromi, Hikita Yuko, Nakao Takafumi, Nakamura Yasuhiro, Muro Takashi, Miura Katsuyuki, Yoshiyama Minoru, Iwao Hiroshi

    HYPERTENSION RESEARCH   32 ( 8 )   675 - 679   2009.08( ISSN:0916-9636

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    DOI: 10.1038/hr.2009.77

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  • Effects of metoprolol on epinephrine-induced takotsubo-like left ventricular dysfunction in non-human primates Reviewed

    IZUMI Yasukatsu, OKATANI Hideaki, SHIOTA Masayuki, NAKAO Takafumi, ISE Ryota, KITO Go, MIURA Katsuyuki, IWAO Hiroshi

    Hypertension research : clinical and experimental : official journal of the Japanese Society of Hypertension   32 ( 5 )   339 - 346   2009.05( ISSN:09169636

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  • Effects of metoprolol on epinephrine-induced takotsubo-like left ventricular dysfunction in non-human primates Reviewed

    Izumi Yasukatsu, Okatani Hideaki, Shiota Masayuki, Nakao Takafumi, Ise Ryota, Kito Go, Miura Katsuyuki, Iwao Hiroshi

    HYPERTENSION RESEARCH   32 ( 5 )   339 - 346   2009.05( ISSN:0916-9636

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    DOI: 10.1038/hr.2009.28

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  • Pharmacogenomics of cardiovascular pharmacology: Molecular network analysis in pleiotropic effects of statin - an experimental elucidation of the pharmacologic action from protein-protein interaction analysis Invited Reviewed

    Shiota Masayuki, Kusakabe Hiromi, Hikita Yuko, Nakao Takafumi, Izumi Yasukatsu, Iwao Hiroshi

    The Japanese Pharmacological Society JOURNAL OF PHARMACOLOGICAL SCIENCES   107 ( 1 )   15 - 19   2008.05( ISSN:1347-8613

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    DOI: 10.1254/jphs.08R01FM

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  • Protein phosphatase 4 catalytic subunit regulates Cdk1 activity and microtubule organization via NDEL1 dephosphorylation. Reviewed

    Toyo-oka K, Mori D, Yano Y, Shiota M, Iwao H, Goto H, Inagaki M, Hiraiwa N, Muramatsu M, Wynshaw-Boris A, Yoshiki A, Hirotsune S

    The Journal of cell biology   180 ( 6 )   1133 - 47   2008.03( ISSN:0021-9525

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    DOI: 10.1083/jcb.200705148

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  • Protein phosphatase 4 catalytic subunit regulates Cdk1 activity and microtubule organization via NDEL1 dephosphorylation Reviewed

    Toyo-oka Kazuhito, Mori Daisuke, Yano Yoshihisa, Shiota Masayuki, Iwao Hiroshi, Goto Hidemasa, Inagaki Masaki, Hiraiwa Noriko, Muramatsu Masami, Wynshaw-Boris Anthony, Yoshiki Atsushi, Hirotsune Shinji

    JOURNAL OF CELL BIOLOGY   180 ( 6 )   1133 - 1147   2008.03( ISSN:0021-9525

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  • Interleukin-10 gene transfer to peritoneal mesothelial cells suppresses peritoneal dissemination of gastric cancer cells due to a persistently high concentration in the peritoneal cavity Reviewed

    Tanaka F., Tominaga K., Shiota M., Ochi M., Kuwamura H., Tanigawa T., Watanabe T., Fujiwara Y., Oshitani N., Higuchi K., Iwao H., Arakawa T.

    CANCER GENE THERAPY   15 ( 1 )   51 - 59   2008.01( ISSN:0929-1903

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    DOI: 10.1038/sj.cgt.7701104

  • Pravastatin induces rat aortic endothelial cell proliferation and migration via activation of PI3K/Akt/mTOR/p70 S6 kinase signaling Reviewed

    Nakao Takafumi, Shiota Masayuki, Tatemoto Yasuyoshi, Izumi Yasukatsu, Iwao Hiroshi

    The Japanese Pharmacological Society JOURNAL OF PHARMACOLOGICAL SCIENCES   105 ( 4 )   334 - 341   2007.12( ISSN:1347-8613

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    DOI: 10.1254/jphs.FP0070682

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  • Growth inhibition of colon cancer cells by transfection of dominant-negative apoptosis signal-regulating kinase-1. Reviewed

    Kuwamura H, Tominaga K, Shiota M, Ashida R, Nakao T, Sasaki E, Watanabe T, Fujiwara Y, Oshitani N, Higuchi K, Ichijo H, Arakawa T, Iwao H

    Oncology reports   17 ( 4 )   781 - 6   2007.04( ISSN:1021-335X

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  • Pleiotropic effects of statins on endothelium and signaling mechanisms

    SHIOTA Masayuki, IZUMI Yasukatsu, NAKAO Takafumi, IWAO Hiroshi

    The Japanese Pharmacological Society, Folia Pharmacologica Japonica   128 ( 3 )   161 - 166   2006.09( ISSN:00155691

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    DOI: 10.1254/fpj.128.161

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  • Important role of apoptosis signal-regulating kinase 1 in ischemia-induced angiogenesis Reviewed

    Izumi Y, Kim-Mitsuyama S, Yoshiyama M, Omura T, Shiota M, Matsuzawa A, Yukimura T, Murohara T, Takeya M, Ichijo H, Yoshikawa J, Iwao H

    ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY   25 ( 9 )   1877 - 1883   2005.09( ISSN:1079-5642

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    DOI: 10.1161/01.ATV.0000174801.76234.bd

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  • Beneficial Effect of Candesartan on Rat Diastolic Heart Failure Reviewed

    Wake R, Kim-Mitsuyama S, Izumi Y, Yoshida K, Izumiya Y, Yukimura T, Shiota M, Yoshiyama M, Yoshikawa J, Iwao H.

    The Japanese Pharmacological Society Journal of pharmacological sciences   98 ( 4 )   372 - 379   2005.08( ISSN:13478613

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    DOI: 10.1254/jphs.FP0050160

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  • Heat stress diminishes gonadotropin receptor expression and enhances susceptibility to apoptosis of rat granulosa cells. Reviewed

    Shimizu T, Ohshima I, Ozawa M, Takahashi S, Tajima A, Shiota M, Miyazaki H, Kanai Y

    Reproduction (Cambridge, England)   129 ( 4 )   463 - 72   2005.04( ISSN:1470-1626

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    DOI: 10.1530/rep.1.00502

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  • Gene, biosynthesis, and function of angiotensinogen. Invited

    Shiota M, Izumi Y, Yukimura T, Iwao H

    Nihon rinsho. Japanese journal of clinical medicine   63 Suppl 3   17 - 21   2005.03( ISSN:0047-1852

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  • Protein tyrosine phosphatase LMW-PTP exhibits distinct roles between vascular endothelial and smooth muscle cells. Reviewed

    Shimizu H, Toyama O, Shiota M, Kim-Mitsuyama S, Miyazaki H

    Journal of receptor and signal transduction research   25 ( 1 )   19 - 33   2005( ISSN:1079-9893

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    DOI: 10.1081/RRS-200047876

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  • Gene expression profile in experimental mesangial proliferative glomerulonephritis. Reviewed

    Izumi Y, Izumiya Y, Shiota M, Yukimura T, Shiojima S, Yamada M, Kadowaki T, Katsuma S, Hirasawa A, Tsujimoto G, Iwao H.

    JOURNAL OF PHARMACOLOGICAL SCIENCES   96   91 - 94   2004

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  • Tyrosine phosphatase epsilonm stimulates migration and survival of porcine aortic endothelial cells by activating c-src. Reviewed

    Nakagawa Y, Yamada N, Shimizu H, Shiota M, Tamura M, Kim-Mitsuyama S, Miyazaki H.

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   325   314 - 319   2004

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  • Feeding is not a more potent zeitgeber than the light-dark cycle in drosophila. Reviewed

    Shiota M, Oishi K, Sakamoto K, Kasamatsu M, Ishida N.

    Neuroreport   15   739 - 743   2004

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  • Protein tyrosine phosphatase PTP20 induces actin cytoskeleton reorganization by dephosphorylating p190 rhogap in rat ovarian granulosa cells stimulated with follicle-stimulating hormone. Reviewed

    Shiota M, Tanihiro T, Nakagawa Y, Aoki N, Ishida N, Miyazaki K, Ullrich A, Miyazaki H.

    Molecular Endocrinology   17   534 - 549   2003

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    Publishing type:Doctoral thesis   Kind of work:Joint Work  

  • Correlation of mitogen-activated protein kinase activities with cell survival and apoptosis in porcine granulosa cells. Reviewed

    Shiota M, Sugai N, Tamura M, Yamaguchi R, Fukushima N, Miyano T, Miyazaki H.

    Zoological Science   20   193 - 201   2003

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    Publishing type:Doctoral thesis   Kind of work:Joint Work  

  • Low m(r) protein tyrosine phosphatase inhibits growth and migration of vascular smooth muscle cells induced by platelet-derived growth factor. Reviewed

    Shimizu H, Shiota M, Yamada N, Miyazaki K, Ishida N, Kim S, Miyazaki H.

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   289   602 - 607   2001

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MISC

  • キーワード解説 HSP70ファミリーの新たな機能 Reviewed

    塩田 正之, 田中 昌子

    (公社)日本薬理学会 日本薬理学雑誌   143 ( 6 )   310 - 312   2014.06( ISSN:0015-5691

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Presentations

Collaborative research (seeds) keywords

  • 抗腫瘍活性を有する細胞膜局在型Hsp70抗体

    Category:Life Science 

  • HSP70結合解析による血中微量タンパク質の単離・同定

    Category:Life Science