研究者詳細 - 若林　和幸

写真a

ワカバヤシ　カズユキ

若林　和幸

WAKABAYASHI Kazuyuki

担当

大学院理学研究科生物学専攻准教授
理学部生物学科

所属

理学研究院

所属キャンパス

杉本キャンパス

担当・職階

大学院理学研究科生物学専攻

准教授 2022年04月 - 継続中
理学部生物学科

准教授 2022年04月 - 継続中

取得学位

学術博士（広島大学）
学術修士（広島大学）

研究分野

ライフサイエンス / 植物分子、生理科学 / 植物生理

研究歴

植物成長の調節機構に関する研究

植物ホルモン、細胞壁、成長個人研究

1900年04月

所属学協会

日本植物生理学会

国内
日本宇宙生物科学会

国内
日本植物学会

国内

職務経歴（学外）

香川大学 -

1989年 - 1991年

学歴

広島大学生物圏科学研究科生物機能科学修士課程

- 1987年
高知大学理学部生物学科

- 1984年

論文

Modification of chemical properties of cell walls by silicon and its role in regulation of the cell wall extensibility in oat leaves.

Hossain MT, Soga K, Wakabayashi K, Kamisaka S, Fujii S, et al.

J Plant Physiol 164 385 - 393 2007年

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掲載種別：研究論文（学術雑誌）
Increase in steryl glycoside levels and stimulation of lipid raft formation in azukii bean epicotyls under hypergravity conditions. 査読

Sakaki T, Koizumi T, Ikeido Y, Soga K, Wakabayashi K, Hoson T

Life Sci Space Res 38 53 - 58 2023年

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掲載種別：研究論文（学術雑誌）国際・国内誌：国際誌
Modification of xyloglucan metabolism during a decrease in cell wall extensibility in 1-Aminocyclopropane-1-carboxylic acid-treated azukii bean epicotyls. 査読

Yamaguchi A, Soga K, Wakabayashi K, Hoson T

plants 12 367 2023年

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掲載種別：研究論文（学術雑誌）国際・国内誌：国際誌

DOI： doi:10.3390/plants12020367

リポジトリURL： http://hdl.handle.net/10466/0002000075
The modification of cell wall properties is involved in the growth inhibition of rice coleoptiles induced by lead stress. 査読

Wakabayashi K, Soga K, Hoson T, Masuda H

life 13 471 2023年

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担当区分：筆頭著者,　責任著者掲載種別：研究論文（学術雑誌）国際・国内誌：国際誌

DOI： doi:10.3390/life13020471

リポジトリURL： http://hdl.handle.net/10466/0002000076
An Arabidopsis PTH2 gene is responsible for gravity resistance supporting plant growth under different gravity conditions 査読

Hattori T, Soga K, Wakabayashi K, Hoson T

life 12 1603 2022年

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掲載種別：研究論文（学術雑誌）国際・国内誌：国際誌

DOI： doi:10.3390/life12101603
Suppression of secondary wall formation in the basal supporting region of Arabidopsis inflorescence stems under microgravity conditions in space. 査読

Tanimura Y, Mabuchi A, Soga K, Wakabayashi K, Hashimoto H, Yano S, Matsumoto S, Kasahara H, Kamada M, Shimazu T, Hashimoto T, Hoson T

Biol Sci Space 36 1 - 8 2022年

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掲載種別：研究論文（学術雑誌）国際・国内誌：国際誌
Suppression of cortical microtubule reorientation and stimulation of cell elongation in Arabidopsis hypocotyls under microgravity conditions in space. 査読

Kato S, Murakami M, Saika R, Soga K, Wakabayashi K, Hashimoto H, Yano S, Matsumoto S, Kasahara H, Kamada M, Shimazu T, Hashimoto T, Hoson T

plants 11 465 2022年

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掲載種別：研究論文（学術雑誌）国際・国内誌：国際誌

DOI： doi:10.3390/plants11030465
Suppression of sugar accumulation in coleoptile and mesocotyl cells by light irradiation to etiolated maize seedlings

Soga-Morimoto A.

Journal of Plant Physiology 260 2021年05月（ ISSN:01761617 ）

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DOI： 10.1016/j.jplph.2021.153409
Microgravity Affects the Level of Matrix Polysaccharide 1,3:1,4-β-Glucans in Cell Walls of Rice Shoots by Increasing the Expression Level of a Gene Involved in Their Breakdown

Wakabayashi K.

Astrobiology 20 ( 7 ) 820 - 829 2020年07月（ ISSN:15311074 ）

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DOI： 10.1089/ast.2019.2140
Mca1 and mca2 are involved in the response to hypergravity in arabidopsis hypocotyls

Hattori T.

Plants 9 ( 5 ) 2020年05月

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DOI： 10.3390/plants9050590
Centrifugal displacement of nuclei in epidermal cells of azuki bean epicotyls

Inui Kenichi, Soga Kouichi, Wakabayashi Kazuyuki, Hoson Takayuki

Biological Sciences in Space 33 ( 0 ) 1 - 6 2019年（ ISSN:09149201 ）（ eISSN:1349967X ）

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We examined the effects of hypergravity on nuclear positioning in epidermal cells of azuki bean (Vigna angularis) epicotyls. The nucleus was positioned almost in the center of the cell under 1 G conditions. When the epicotyls were exposed to basipetal hypergravity by centrifugation, the nucleus was displaced toward the centrifugal side of the cell in a linear dose-response manner. The nuclear displacement started within 20 min by exposure to basipetal hypergravity at 300 G, and reached ca. 35% from the centrifugal edge of the cell after 2 h. The displaced nucleus recentered by removal of hypergravity stimuli. The nucleus was also displaced by acropetal hypergravity at the similar degree as basipetal hypergravity. The displacement by hypergravity of the nucleus was stimulated, when actin filaments were disrupted by cytochalasin D treatment. These results suggest that the positioning of the nucleus in epidermal cells is maintained by actin filaments, which is affected by gravity in azuki bean epicotyls.

DOI： 10.2187/bss.33.1

CiNii Article
Growth and cortical microtubule dynamics in shoot organs under microgravity and hypergravity conditions. 査読

Soga K, Wakabayashi K, Hoson T

Plant signaling & behavior 13 ( 1 ) e1422468 2018年01月（ ISSN:1559-2316 ）

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DOI： 10.1080/15592324.2017.1422468

PubMed
Modification of growth anisotropy and cortical microtubule dynamics in Arabidopsis hypocotyls grown under microgravity conditions in space 査読

Kouichi Soga, Chiaki Yamazaki, Motoshi Kamada, Naoki Tanigawa, Haruo Kasahara, Sachiko Yano, Kei H. Kojo, Natsumaro Kutsuna, Takehide Kato, Takashi Hashimoto, Toshihisa Kotake, Kazuyuki Wakabayashi, Takayuki Hoson

PHYSIOLOGIA PLANTARUM 162 ( 1 ) 135 - 144 2018年01月（ ISSN:0031-9317 ）（ eISSN:1399-3054 ）

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掲載種別：研究論文（学術雑誌）

We carried out a space experiment, denoted as Aniso Tubule, to examine the effects of microgravity on the growth anisotropy and cortical microtubule dynamics in Arabidopsis hypocotyls, using lines in which microtubules are visualized by labeling tubulin or microtubule-associated proteins (MAPs) with green fluorescent protein (GFP). In all lines, GFP-tubulin6 (TUB6)-, basic proline-rich protein1 (BPP1)-GFP- and spira1-like3 (SP1L3)-GFP-expressing using a constitutive promoter, and spiral2 (SPR2)-GFP- and GFP-65 kDa MAP-1 (MAP65-1)-expressing using a native promoter, the length of hypocotyls grown under microgravity conditions in space was longer than that grown at 1 g conditions on the ground. In contrast, the diameter of hypocotyls grown under microgravity conditions was smaller than that of the hypocotyls grown at 1 g. The percentage of cells with transverse microtubules was increased under microgravity conditions, irrespective of the lines. Also, the average angle of the microtubules with respect to the transverse cell axis was decreased in hypocotyls grown under microgravity conditions. When GFP fluorescence was quantified in hypocotyls of GFP-MAP65-1 and SPR2-GFP lines, microgravity increased the levels of MAP65-1, which appears to be involved in the maintenance of transverse microtubule orientation. However, the levels of SPR2 under microgravity conditions were comparable to those at 1 g. These results suggest that the microgravity-induced increase in the levels of MAP65-1 is involved in increase in the transverse microtubules, which may lead to modification of growth anisotropy, thereby developing longer and thinner hypocotyls under microgravity conditions in space.

DOI： 10.1111/ppl.12640

PubMed
Dynamics of Actin Filaments in Epidermal Cells of Azuki Bean Epicotyls under Hypergravity Conditions

Tanabe Hiroyo, Soga Kouichi, Wakabayashi Kazuyuki, Hoson Takayuki

Biological Sciences in Space 32 ( 0 ) 11 - 16 2018年（ ISSN:09149201 ）（ eISSN:1349967X ）

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The effects of hypergravity on growth and dynamics of actin filaments were examined in azuki bean (Vigna angularis) epicotyls. Elongation growth occurred mainly in the apical region of epicotyls, which was inhibited by hypergravity at 300 G. The density of actin filaments in epidermal cells decreased from the apical to the basal regions of epicotyls, irrespective of the gravitational conditions, and in the apical region of epicotyls, hypergravity decreased the density. Actin filaments were arranged with longitudinal or radial direction in the epidermal cells of apical region of 1 G-grown epicotyls. On the other hand, actin filaments with transverse direction were observed in basal region of epicotyls grown at 1 G. Similar changes in the arrangement of actin filaments toward the basal region were observed even under hypergravity conditions. Hypergravity had no effects on the growth and reorientation of cortical microtubules, when actin filaments were disrupted by cytochalasin D treatment. These results suggest that modification of dynamics of actin filaments is responsible for reorientation of cortical microtubules, which leads to inhibition of elongation growth in azuki bean epicotyls under hypergravity conditions.

DOI： 10.2187/bss.32.11

CiNii Article
Persistence of plant hormone levels in rice shoots grown under microgravity conditions in space: its relationship to maintenance of shoot growth 査読

Kazuyuki Wakabayashi, Kouichi Soga, Takayuki Hoson, Toshihisa Kotake, Mikiko Kojima, Hitoshi Sakakibara, Takashi Yamazaki, Akira Higashibata, Noriaki Ishioka, Toru Shimazu, Motoshi Kamada

PHYSIOLOGIA PLANTARUM 161 ( 2 ) 285 - 293 2017年10月（ ISSN:0031-9317 ）（ eISSN:1399-3054 ）

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掲載種別：研究論文（学術雑誌）

We investigated the effects of microgravity environment on growth and plant hormone levels in dark-grown rice shoots cultivated in artificial 1 g and microgravity conditions on the International Space Station (ISS). Growth of microgravity-grown shoots was comparable to that of 1 g-grown shoots. Endogenous levels of indole-3-acetic acid (IAA) in shoots remained constant, while those of abscisic acid (ABA), jasmonic acid (JA), cytokinins (CKs) and gibberellins (GAs) decreased during the cultivation period under both conditions. The levels of auxin, ABA, JA, CKs and GAs in rice shoots grown under microgravity conditions were comparable to those under 1 g conditions. These results suggest microgravity environment in space had minimal impact on levels of these plant hormones in rice shoots, which may be the cause of the persistence of normal growth of shoots under microgravity conditions. Concerning ethylene, the expression level of a gene for 1-aminocyclopropane-1-carboxylic acid (ACC) synthase, the key enzyme in ethylene biosynthesis, was reduced under microgravity conditions, suggesting that microgravity may affect the ethylene production. Therefore, ethylene production may be responsive to alterations of the gravitational force.

DOI： 10.1111/ppl.12591

PubMed
Phenotypic screening of Arabidopsis T-DNA insertion lines for cell wall mechanical properties revealed ANTHOCYANINLESS2, a cell wall-related gene 査読

Atsushi Mabuchi, Kouichi Soga, Kazuyuki Wakabayashi, Takayuki Hoson

JOURNAL OF PLANT PHYSIOLOGY 191 29 - 35 2016年02月（ ISSN:0176-1617 ）（ eISSN:1618-1328 ）

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掲載種別：研究論文（学術雑誌）

We performed a phenotypic screening of confirmed homozygous T-DNA insertion lines in Arabidopsis for cell wall extensibility, in an attempt to identify genes involved in the regulation of cell wall mechanical properties. Seedlings of each line were cultivated and the cell wall extensibility of their hypocotyls was measured with a tensile tester. Hypocotyls of lines with known cell wall-related genes showed higher or lower extensibility than those of the wild-type at high frequency, indicating that the protocol used was effective. In the first round of screening of randomly selected T-DNA insertion lines, we identified ANTHOCYANINLESS2 (ANL2), a gene involved in the regulation of cell wall mechanical properties. In the anl2 mutant, the cell wall extensibility of hypocotyls was significantly lower than that of the wild-type. Levels of cell wall polysaccharides per hypocotyl, particularly cellulose, increased in anl2. Microarray analysis showed that in anl2, expression levels of the major peroxidase genes also increased. Moreover, the activity of ionically wall-bound peroxidases clearly increased in anl2. The activation of peroxidases as well as the accumulation of cell wall polysaccharides may be involved in decreased cell wall extensibility. The approach employed in the present study could contribute to our understanding of the mechanisms underlying the regulation of cell wall mechanical properties. (C) 2016 Elsevier GmbH. All rights reserved.

DOI： 10.1016/j.jplph.2015.11.011

PubMed
Roles of MAP65-1 and BPP1 in Gravity Resistance of Arabidopsis hypocotyls

Murakami Mana, Soga Kouichi, Kotake Toshihisa, Kato Takehide, Hashimoto Takashi, Wakabayashi Kazuyuki, Hoson Takayuki

Biological Sciences in Space 30 ( 0 ) 1 - 7 2016年（ ISSN:09149201 ）（ eISSN:1349967X ）

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The present experiment aims to clarify the roles of 65 kDa microtubule-associated protein-1 (MAP65-1) and basic proline-rich protein1 (BPP1), which are involved in the maintenance of transverse microtubule orientation, in gravity resistance, using green fluorescent protein (GFP)-expressing Arabidopsis lines. Hypergravity at 300 G inhibited elongation growth and promoted lateral expansion of epidermal cells in the subapical region of hypocotyls in GFP-MAP65-1 line expressing by native promoter and BPP1-GFP line expressing by a constitutive cauliflower mosaic virus 35S promoter. In BPP1-GFP line, hypergravity showed smaller effects on modification of growth anisotropy than wild type. Also, hypergravity induced reorientation of cortical microtubules from transverse to longitudinal directions in both lines. However, in BPP1-GFP line, hypergravity showed smaller effects on reorientation of cortical microtubules. When the expression levels of MAP65-1 were determined by analyzing GFP fluorescence in hypocotyls of GFP-MAP65-1 line, hypergravity decreased the levels of MAP65-1 in the subapical region, where hypergravity modified growth anisotropy and orientation of cortical microtubules. These results indicate that the regulation of levels of MAP65-1 and BPP1 is involved in the hypergravity-induced reorientation of cortical microtubules, which may lead to modification of growth anisotropy, thereby developing a tough body against the gravitational force in Arabidopsis hypocotyls.

DOI： 10.2187/bss.30.1

CiNii Article
Suppression of Hydroxycinnamate Network Formation in Cell Walls of Rice Shoots Grown under Microgravity Conditions in Space 査読

Kazuyuki Wakabayashi, Kouichi Soga, Takayuki Hoson, Toshihisa Kotake, Takashi Yamazaki, Akira Higashibata, Noriaki Ishioka, Toru Shimazu, Keiji Fukui, Ikuko Osada, Haruo Kasahara, Motoshi Kamada

PLOS ONE 10 ( 9 ) e0137992 2015年09月（ ISSN:1932-6203 ）

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掲載種別：研究論文（学術雑誌）

Network structures created by hydroxycinnamate cross-links within the cell wall architecture of gramineous plants make the cell wall resistant to the gravitational force of the earth. In this study, the effects of microgravity on the formation of cell wall-bound hydroxycinnamates were examined using etiolated rice shoots simultaneously grown under artificial 1 g and microgravity conditions in the Cell Biology Experiment Facility on the International Space Station. Measurement of the mechanical properties of cell walls showed that shoot cell walls became stiff during the growth period and that microgravity suppressed this stiffening. Amounts of cell wall polysaccharides, cell wall-bound phenolic acids, and lignin in rice shoots increased as the shoot grew. Microgravity did not influence changes in the amounts of cell wall polysaccharides or phenolic acid monomers such as ferulic acid (FA) and p-coumaric acid, but it suppressed increases in diferulic acid (DFA) isomers and lignin. Activities of the enzymes phenylalanine ammonia-lyase (PAL) and cell wall-bound peroxidase (CW-PRX) in shoots also increased as the shoot grew. PAL activity in microgravity-grown shoots was almost comparable to that in artificial 1 g-grown shoots, while CW-PRX activity increased less in microgravity-grown shoots than in artificial 1 g-grown shoots. Furthermore, the increases in expression levels of some class III peroxidase genes were reduced under microgravity conditions. These results suggest that a microgravity environment modifies the expression levels of certain class III peroxidase genes in rice shoots, that the resultant reduction of CW-PRX activity may be involved in suppressing DFA formation and lignin polymerization, and that this suppression may cause a decrease in cross-linkages within the cell wall architecture. The reduction in intra-network structures may contribute to keeping the cell wall loose under microgravity conditions.

DOI： 10.1371/journal.pone.0137992

PubMed
EFFECTS OF LEAD TOXICITY ON GROWTH AND CELL WALL EXTENSIBILITY IN RICE SEEDLINGS 査読

Mohammad Talim Hossain, Kouichi Soga, Kazuyuki Wakabayashi, Takayuki Hoson

BANGLADESH JOURNAL OF BOTANY 44 ( 2 ) 333 - 336 2015年06月（ ISSN:0253-5416 ）（ eISSN:2079-9926 ）

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掲載種別：研究論文（学術雑誌）

The effect of lead (Pb) on growth and mechanical properties of cell wall was investigated in rice seedlings. Caryopses of rice were germinated and grown in various concentrations of lead nitrate for 5 days at 25 degrees C in the dark. Growth of rice seedlings was suppressed by Pb ions; significant suppression was caused by low concentration of Pb as 1 mu M. Growth suppression was prominent in roots; but not clear in shoot organs, such as coleoptiles or first leaves, suggesting that roots are the primary target of Pb toxicity. The analysis of the cell wall extensibility of rice roots grown in Pb solution indicated that the cell wall extensibility was greatly decreased with increased concentration of Pb ions. These results suggest that Pb may influence the synthesis of cell wall polysaccharides, thereby decreasing the cell wall extensibility, resulting in growth suppression in rice roots.
Role of the plant cell wall in gravity resistance 査読

Takayuki Hoson, Kazuyuki Wakabayashi

PHYTOCHEMISTRY 112 84 - 90 2015年04月（ ISSN:0031-9422 ）

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Gravity resistance, mechanical resistance to the gravitational force, is a principal graviresponse in plants, comparable to gravitropism. The cell wall is responsible for the final step of gravity resistance. The gravity signal increases the rigidity of the cell wall via the accumulation of its constituents, polymerization of certain matrix polysaccharides due to the suppression of breakdown, stimulation of cross-link formation, and modifications to the wall environment, in a wide range of situations from microgravity in space to hypergravity. Plants thus develop a tough body to resist the gravitational force via an increase in cell wall rigidity and the modification of growth anisotropy. The development of gravity resistance mechanisms has played an important role in the acquisition of responses to various mechanical stresses and the evolution of land plants. (C) 2014 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.phytochem.2014.08.022

PubMed
Effects of gravistimuli on osmoregulation in azuki bean epicotyls 査読

Yan Zhang, Kouichi Soga, Kazuyuki Wakabayashi, Takayuki Hoson

ADVANCES IN SPACE RESEARCH 51 ( 3 ) 458 - 464 2013年02月（ ISSN:0273-1177 ）

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掲載種別：研究論文（学術雑誌）

The effects of hypergravity on growth and osmoregulation were examined in dark-grown azuki bean epicotyls. Elongation growth of epicotyls was promptly suppressed by hypergravity at 300g. On the contrary, the increase in fresh weight of epicotyls during incubation was not suppressed by hypergravity at 300g at least up to 6 h. Also, the level of total osmotic solutes increased during epicotyl growth for 6 h, which was not affected by hypergravity. These results suggest that azuki bean epicotyls are capable of maintaining osmoregulation even under 300g conditions for a short period. On the other hand, the increase in fresh weight of epicotyls was suppressed, in addition to suppression of elongation growth, when seedlings were treated with 300g for 24 h. The increase in level of total osmotic solutes was also inhibited by 24 h hypergravity treatment, which was accounted by the reduced levels of organic solutes, such as sugars and amino acids. Furthermore, the dry weight of seeds decreased during incubation for 24 h, but the decrease was inhibited by hypergravity at 300g. Hypergravity treatment at 300g for 24 h also increased the pH value of apoplastic solution in epicotyls. Taken together, these results suggest that the translocation of organic solutes from the seed to epicotyls is inhibited by prolonged hypergravity treatment, which may underlie the suppression of epicotyl growth, and that the breakdown of H+ gradient across the plasma membrane in epicotyl cells may be at least partly involved in the reduction of organic solute accumulation under hypergravity conditions. (C) 2012 COSPAR. Published by Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.asr.2012.09.013
Phenylalanine ammonia-lyase and cell wall peroxidase are cooperatively involved in the extensive formation of ferulate network in cell walls of developing rice shoots 査読

Kazuyuki Wakabayashi, Kouichi Soga, Takayuki Hoson

JOURNAL OF PLANT PHYSIOLOGY 169 ( 3 ) 262 - 267 2012年02月（ ISSN:0176-1617 ）

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掲載種別：研究論文（学術雑誌）

The relationship between the formation of cell wall-bound ferulic acid (FA) and diferulic acid (DFA) and the change in activities of phenylalanine ammonia-lyase (PAL) and cell wall-bound peroxidase (CW-PRX) was studied in rice shoots. The length and the fresh mass of shoots increased during the growth period from day 4 to 6, while coleoptiles ceased elongation growth on day 5. The amounts of FA and DFA isomers as well as cell wall polysaccharides continued to increase during the whole period. The activities of PAL and CW-PRX greatly increased in the same manner during the period. There were close correlations between the PAL activity and ferulate content or between the CW-PRX activity and DFA content. The expression levels of investigated genes for PAL and putative CW-PRX showed good accordance with the activities of these enzymes. These results suggest that increases in PAL and CW-PRX activities are cooperatively involved in the formation of ferulate network in cell walls of rice shoots and that investigated genes may be, at least in part, associated with the enzyme activities. The substantial increase in such network probably causes the maturation of cell walls and thus the cessation of elongation growth of coleoptiles. (C) 2011 Elsevier GmbH. All rights reserved.

DOI： 10.1016/j.jplph.2011.10.002

PubMed
Excellent Thermal Control Ability of Cell Biology Experiment Facility (CBEF) for Ground-Based Experiments and Experiments Onboard the Kibo Japanese Experiment Module of International Space Station

Yano Sachiko, Masuda Daisuke, Kasahara Haruo, Omori Katsunori, Higashibata Akira, Asashima Makoto, Ohnishi Takeo, Yatagai Fumio, Kamisaka Seiichiro, Furusawa Toshiharu, Higashitani Atsushi, Majima Hideyuki J., Nikawa Takeshi, Wakabayashi Kazuyuki, Takahashi Hideyuki, Suzuki Hiromi, Shimazu Toru, Fukui Keiji, Hattori Atsuhiko, Tanigaki Fumiaki, Shirakawa Masaki, Nakamura Tai, Yoshimura Yoshinori, Suzuki Nobuo, Ishioka Noriaki

Biological Sciences in Space 26 ( 0 ) 12 - 20 2012年（ ISSN:09149201 ）（ eISSN:1349967X ）

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The Japan Aerospace Exploration Agency (JAXA) has been developing several life science hardware components for the Japanese experiment module ‘Kibo’ in the International Space Station (ISS). The Cell Biology Experiment Facility (CBEF) is one of these. It contains an environmental control system to regulate variables such as the temperature and humidity. It is also equipped with a centrifuge and has the ability to culture bio-specimens in a temperature range of 15 to 40°C. In biological experiments that include in vitro cell culture, temperature control is a key factor in the success of the experiment. Thus, we first examined the temperature data in the CBEF on the ground while changing the room temperature and cooling water temperature to investigate the ability of the CBEF. Based on the ground experiment data, we carried out life science experiments onboard Kibo. Judging from the temperature data in the CBEF under microgravity, 12 life science experiments were successfully conducted up until 2011. Considering our present data, we strongly believe that the CBEF is a functional incubator in the ISS and will contribute to future experiments.

DOI： 10.2187/bss.26.12

CiNii Article
Objectives, Outlines, and Preparation for the Resist Tubule Space Experiment to Understand the Mechanism of Gravity Resistance in Plants

HOSON Takayuki, AKAMATSU Haruhiko, SOGA Kouichi, WAKABAYASHI Kazuyuki, HASHIMOTO Hirofumi, YAMASHITA Masamichi, HASEGAWA Katsuya, YANO Sachiko, OMORI Katsunori, ISHIOKA Noriaki, MATSUMOTO Shohei, KASAHARA Haruo, SHIMAZU Toru, A. BABA Shoji, HASHIMOTO Takashi

TRANSACTIONS OF THE JAPAN SOCIETY FOR AERONAUTICAL AND SPACE SCIENCES, AEROSPACE TECHNOLOGY JAPAN 10 ( ists28 ) Tp_1 - Tp_5 2012年（ ISSN:18840485 ）（ eISSN:18840485 ）

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Gravity resistance is a principal graviresponse in plants. In resistance to hypergravity, the gravity signal may be perceived by the mechanoreceptors located on the plasma membrane, and then transformed and transduced via the structural continuum or physiological continuity of cortical microtubules-plasma membrane-cell wall, leading to an increase in the cell wall rigidity as the final response. The Resist Tubule experiment, which will be conducted in the Kibo Module on the International Space Station, aims to confirm that this hypothesis is applicable to resistance to 1 G gravity. There are two major objectives in the Resist Tubule experiment. One is to quantify the contributions of cortical microtubules to gravity resistance using Arabidopsis tubulin mutants with different degrees of defects. Another objective is to analyze the modifications to dynamics of cortical microtubules and membrane rafts under microgravity conditions on-site by observing green fluorescent protein (GFP)-expressing Arabidopsis lines with the fluorescence microscope in the Kibo. We have selected suitable mutants, developed necessary hardware, and fixed operation procedure for the experiment.

DOI： 10.2322/tastj.10.tp_1

CiNii Article
Cell wall oxalate oxidase modifies the ferulate metabolism in cell walls of wheat shoots.

Wakabayashi K, Soga K, Hoson T

J Plant Physiol (doi: 10.1016/j.jplph.2011.05.010) 2011年

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掲載種別：研究論文（学術雑誌）
Transient increase in the levels of γ-tubulin complex and katanin are responsible for reorientation by ethylene and hypergravity of cortical microtubules. 査読

Soga K, Yamaguchi A, Kotake T, Wakabayashi K, Hoson T

Plant signaling & behavior 5 ( 11 ) 1480 - 1482 2010年11月（ ISSN:1559-2316 ）

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DOI： 10.1016/j.jplph.2010.04.001

PubMed
Gravity-induced modifications to development in hypocotyls of Arabidopsis tubulin mutants.

Matsumoto S, Kumasaki S, Soga K, Wakabayashi K, Hashimoto T, Hoson T

Plant Physiol 152 918 - 926 2010年

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掲載種別：研究論文（学術雑誌）
Fucosylated high molecular mass but not non-fucosylated low molecular mass xyloglucans undergo an extensive depolymerization in cell walls of azuki bean epicotyls.

Arai K, Wakabayashi K, Soga K, Hoson T

J Plant Physiol 167 800 - 804 2010年

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掲載種別：研究論文（学術雑誌）
1-Aminocyclopropane-1-carboxylic acid (ACC)-induced reorientation of cortical microtubules is accompanied by a transient increase in the transcript levels of gamma-tubulin complex and katanin genes in azuki bean epicotyls.

Soga K, Yamaguchi A, Kotake T, Wakabayashi K, Hoson T

J Plant Physiol 167 1165 - 1171 2010年

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掲載種別：研究論文（学術雑誌）
Cortical microtubules are responsible for gravity resistance in plants 査読

Takayuki Hoson, Shouhei Matsumoto, Kouichi Soga, Kazuyuki Wakabayashi

Plant Signaling and Behavior 5 ( 6 ) 752 - 754 2010年（ ISSN:1559-2324 ）

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掲載種別：研究論文（学術雑誌）

Mechanical resistance to the gravitational force is a principal gravity response in plants distinct from gravitropism. In the final step of gravity resistance, plants increase the rigidity of their cell walls. Here we discuss the role of cortical microtubules, which sustain the function of the cell wall, in gravity resistance. Hypocotyls of Arabidopsis tubulin mutants were shorter and thicker than the wild-type, and showed either left-handed or right-handed helical growth at 1 g. The degree of twisting phenotype was intensified under hypergravity conditions. Hypergravity also induces reorientation of cortical microtubules from transverse to longitudinal directions in epidermal cells. In tubulin mutants, the percentage of cells with longitudinal microtubules was high even at 1 g, and it was further increased by hypergravity. The left-handed helical growth mutants had right-handed microtubule arrays, whereas the right-handed mutant had left-handed arrays. Moreover, blockers of mechanoreceptors suppressed both the twisting phenotype and reorientation of microtubules in tubulin mutants. These results support the hypothesis that cortical microtubules play an essential role in maintenance of normal growth phe-notype against the gravitational force, and suggest that mechanoreceptors are involved in signal perception in gravity resistance. Space experiments will confirm whether this view is applicable to plant resistance to 1 g gravity, as to the resistance to hypergravity. © 2010 Landes Bioscience.

DOI： 10.4161/psb.5.6.11706

PubMed
The transcript level of katanin gene is increased transiently in response to changes in gravitational conditions in azuki bean epicotyls.

Soga K, Kotake T, Wakabayashi K, Kamisaka S, Hoson T

Biol Sci Space 23 23 - 28 2009年

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掲載種別：研究論文（学術雑誌）
Cellular basis of growth suppression by submergence in azuki bean epicotyls.

Ooume K, Inoue Y, Soga K, Wakabayashi K, Fujii S, Yamamoto R, Hoson T

Ann Bot 103 325 - 332 2009年

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掲載種別：研究論文（学術雑誌）
Cell wall-bound peroxidase activity and lignin formation in azuki bean epicotyls grown under hypergravity conditions.

Wakabayashi K, Nakano S, Soga K, Hoson T

J Plant Physiol 166 947 - 954 2009年

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掲載種別：研究論文（学術雑誌）
Role of the Cell Wall-Sustaining System in Gravity Resistance in Plants

Hoson Takayuki, Soga Kouichi, Wakabayashi Kazuyuki

Biological Sciences in Space 23 ( 3 ) 131 - 136 2009年（ ISSN:09149201 ）（ eISSN:1349967X ）

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Gravity resistance is one of two principal gravity responses in plants, comparable to gravitropism. In the final step of gravity resistance, plants increase the rigidity of their cell walls via modifications to the metabolism. Various constituents of the plasma membrane and the cytoskeleton play an important role in sustaining functions of the cell wall in gravity resistance. Mechanoreceptors located on the plasma membrane are involved in the perception of gravity signal. The perceived signal may be, at least partly, transformed and transduced via membrane sterol rafts, depending on its magnitude. Cellulose synthases and proton pumps are responsible for modifications to the cell wall metabolism and the apoplastic environment, respectively. On the other hand, the reorientation of cortical microtubules contributes to modification of growth anisotropy, which is related to gravity resistance. Also, microtubule-associated proteins are important in maintenance of the structure and induction of the reorientation of cortical microtubules. Gravity resistance in plants is thus mediated by the structural continuum or physiological continuity of cortical microtubules-plasma membrane-cell wall.

DOI： 10.2187/bss.23.131

CiNii Article
beta-1,3:1,4-Glucan synthase activity in rice seedlings under water.

Kimpara T, Aohara T, Soga K, Wakabayashi K, Hoson T, Tsumuraya Y, Kotake T

Ann Bot 102 221 - 226 2008年

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掲載種別：研究論文（学術雑誌）
Transient increase in the transcript levels ofgamma-tubulin complex genes during reorientation of cortical microtubules by gravity in azuki bean (Vigna angularis) epicotyls.

Soga K, Kotake T, Wakabayashi K, Kamisaka S, Hoson T

J Plant Res 121 493 - 498 2008年

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掲載種別：研究論文（学術雑誌）
Up-regulation of expression of tubulin genes and roles of microtubules in hypergravity-induced growth modification in Arabidopsis hypocotyls.

Matsumoto S, Saito Y, Kumasaki S, Soga K, Wakabayashi K, Hoson T

Adv Space Res 39 1176 - 1181 2007年

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掲載種別：研究論文（学術雑誌）
Effects of hypergravity on expression of XTH genes in azuki bean epicotyls.

Soga K, Wakabayashi K, Kamisaka S, Hoson T

Physiol Plant 131 332 - 340 2007年

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掲載種別：研究論文（学術雑誌）
Changes in membrane lipid composition in azuki bean epicotyls under hypergravity conditions: Possible role of membrane sterols in gravity resistance.

Koizumi T, Sakaki T, Usui S, Soga K, Wakabayashi K, Hoson T

Adv Space Res 39 1198 - 1203 2007年

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掲載種別：研究論文（学術雑誌）
Modifications of xyloglucan metabolism in azuki bean epicotyls under hypergravity conditions.

Soga K, Arai K, Wakabayashi K, Kamisaka S, Hoson T

Adv Space Res 39 1204 - 1209 2007年

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掲載種別：研究論文（学術雑誌）
Different cell wall polysaccharides are responsible for gravity resistance in the upper and the basal regions of azuki bean epicotyls.

Nakano S, Soga K, Wakabayashi K, Hoson T

Biol Sci Space 21 113 - 116 2007年

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掲載種別：研究論文（学術雑誌）
The Outline and Significance of the Resist Wall Experiment: Role of Microtubule-Membrane-Cell Wall Continuum in Gravity Resistance in Plants

Hoson Takayuki, Matsumoto Shouhei, Soga Kouichi, Wakabayashi Kazuyuki, Hashimoto Takashi, Sonobe Seiji, Muranaka Toshiya, Kamisaka Seiichiro, Kamada Motoshi, Omori Katsunori, Ishioka Noriaki, Shimazu Toru

Biological Sciences in Space 21 ( 3 ) 56 - 61 2007年（ ISSN:09149201 ）（ eISSN:1349967X ）

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Resistance to the gravitational force is one of two major graviresponses in plants. However, only limited information has been obtained for its mechanism. The Resist Wall experiment aims to examine the role of the cortical microtubule-plasma membrane-cell wall continuum in gravity resistance, thereby clarifying its mechanism. For this purpose, we will cultivate Arabidopsis mutants defective in organization of cortical microtubules (tua6) or synthesis of membrane sterols (hmg1) as well as the wild type Columbia under microgravity and 1G conditions in the European Modular Cultivation System on the International Space Station up to reproductive stage, and compare phenotypes on growth and development using video images. These mutants are unable to form the normal cell wall and show disordered growth pattern on Earth. However, it is expected that the defects of such mutants are rescued and they can grow and develop more or less normally under microgravity in space, where formation of the tough cell wall is not required. We will also analyze changes in expression of genes involved in formation of the continuum and properties of related cellular components under microgravity conditions. The results of the Resist Wall experiment will clarify the molecular mechanism of gravity resistance and benefit efficient plant production not only in space but on Earth.

DOI： 10.2187/bss.21.56

CiNii Article
Alteration of molecular size of xyloglucans in cell walls of azuki bean epicotyls

ARAI Kuninori, SOGA Kouichi, WAKABAYASHI Kazuyuki, HOSON Takayuki

119 117 2006年12月（ ISSN:09189440 ）

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CiNii Article
Effects of gravity on growth phenotype in MAPs mutants of Arabidopsis

KUMASAKI Saori, MATSUMOTO Shouhei, SOGA Kouichi, WAKABAYASHI Kazuyuki, ABE Tatsuya, ISHIDA Takashi, HASHIMOTO Takashi, HOSON Takayuki

119 156 - 157 2006年12月（ ISSN:09189440 ）

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CiNii Article
Successive changes in the structure and metabolism of cell wall polysaccharides along azuki bean epicotyls

NAKANO Saho, SOGA Kouichi, WAKABAYASHI Kazuyuki, HOSON Takayuki

119 118 2006年12月（ ISSN:09189440 ）

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CiNii Article
EFFECTS OF GRAVITY ON GROWTH PHENOTYPE OF TUBULIN MUTANTS IN ARABIDOPSIS

MATSUMOTO Shouhei, KUMASAKI Saori, SOGA Kouichi, WAKABAYASHI Kazuyuki, ABE Tatsuya, ISHIDA Takashi, HASHIMOTO Takashi, HOSON Takayuki

119 156 2006年12月（ ISSN:09189440 ）

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CiNii Article
Hypergravity induces reorientation of cortical microtubules and modifies growth anisotropy in azuki bean epicotyls.

Soga K, Wakabayashi K, Kamisaka S, Hoson T

Planta 224 1485 - 1494 2006年

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掲載種別：研究論文（学術雑誌）
Changes in levels of cell wall constituents in wheat seedlings grown under continuous hypergravity conditions.

Wakabayashi K, Soga K, Kamisaka S, Hoson T

Adv Space Res 36 1292 - 1297 2005年

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掲載種別：研究論文（学術雑誌）
Hypergravity inhibits elongation growth of azuki bean epicotyls independently of the direction of stimuli.

Soga K, Wakabayashi K, Kamisaka S, Hoson T

Adv Space Res 36 1269 - 1276 2005年

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掲載種別：研究論文（学術雑誌）
Cellular basis for the automorphic curvature of rice coleoptiles on a three-dimensional clinostat: Possible involvement of reorientation of cortical microtubules.

Saiki M, Fujita H, Soga K, Wakabayashi K, Kamisaka S et al.

J. Plant Res. 118 199 - 205 2005年

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掲載種別：研究論文（学術雑誌）
Increase in the level of arabinoxylan-hydroxycinnamate network in cell walls of wheat coleoptiles grown under continuous hypergravity conditions.

Wakabayashi K, Soga K, Kamisaka S, Hoson T

Physiol. Plant. 125 127 - 134 2005年

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掲載種別：研究論文（学術雑誌）
Mechanoreceptors rather than sedimentable amyloplasts perceive the gravity signal in hypergravity-induced inhibition of root growth in azuki bean.

Soga K, Wakabayashi K, Kamisaka S, Hoson T

Funct. Plant Biol. 32 175 - 179 2005年

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掲載種別：研究論文（学術雑誌）
Xyloglucan oligosaccharides cause cell wall loosening by enhancing xyloglucan endotransglucosylase/hydrolase activity in azuki bean epicotyls.

Kaku T, Tabuchi A, Wakabayashi K, Hoson T

Plant Cell Physiol. 45 77 - 82 2004年

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掲載種別：研究論文（学術雑誌）
Graviperception in growth inhibition of plant shoots under hypergravity conditions produced by centrifugation is independent of that in gravitropism and may involve mechanoreceptors.

Soga K, Wakabayashi K, Kamisaka S, Hoson T

Planta 218 1054 - 1061 2004年

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掲載種別：研究論文（学術雑誌）
Cell wall changes involved in the automorphic curvature of rice coleoptiles under microgravity conditions in space.

Hoson T, Soga K, Mori R, Saiki M, Nakamura Y, et al.

J. Plant Res. 117 449 - 455 2004年

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掲載種別：研究論文（学術雑誌）
Purification and properties of apoplastic amylase from oat (Avena sativa) seedlings.

Uno-Okamura K, Soga K, Wakabayashi K, Kamisaka S, Hoson T

Physiol. Plant. 121 117 - 123 2004年

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掲載種別：研究論文（学術雑誌）
Xyloglucan breakdown during cotton fiber development.

Tokumoto H, Wakabayashi K, Kamisaka S, Hoson T

J. Plant Physiol. 160 1411 - 1414 2003年

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掲載種別：研究論文（学術雑誌）
Hypergravity-induced changes in gene expression in Arabidopsis hypocotyls.

Yoshioka R, Soga K, Wakabayashi K, Takeba G, Hoson T

Adv. Space Res. 31 2187 - 2193 2003年

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掲載種別：研究論文（学術雑誌）
Growth and cell wall changes in rice roots during spaceflight.

Hoson T, Soga K, Wakabayashi K, Kamisaka S, Tanimoto E

Plant Soil 255 19 - 26 2003年

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掲載種別：研究論文（学術雑誌）
Growth restoration in azuki bean and maize seedlings by removal of hypergravity stimuli.

Soga K, Wakabayashi K, Kamisaka S, Hoson T

Adv. Space Res. 31 2269 - 2274 2003年

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掲載種別：研究論文（学術雑誌）
Temperature modulates the cell wall mechanical properties of rice coleoptiles by altering the molecular mass of hemicellulosicpolysaccharides.

Nakamura Y, Wakabayashi K, Hoson T

Physiol. Plant. 118 597 - 604 2003年（ ISSN:319317 ）

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掲載種別：研究論文（学術雑誌）
Methyl de-esterification as a major factor regulating the extent of pectin depolymerization during fruit ripening: a comparison of the action of avocado (Persea americana) and tomato (Lycopersicon esculentum) polygalacturonases.

Wakabayashi K, Hoson T, Huber DJ

J. Plant Physiol. 160 667 - 673 2003年

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掲載種別：研究論文（学術雑誌）
Stimulation of elongation growth and cell wall loosening in rice coleoptiles under microgravity conditions in space.

Hoson T, Soga K, Mori R, Saiki M, Nakamura Y, Wakabayashi K, Kamisaka S

Plant Cell Physiol. 43 1067 - 1071 2002年（ ISSN:320781 ）

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掲載種別：研究論文（学術雑誌）
Effects of temperature on the cell wall and osmotic properties in dark-grown rice and azuki bean seedlings.

Nakamura Y, Wakabayashi K, Kamisaka S, Hoson T

J. Plant Res. 115 455 - 461 2002年（ ISSN:9189440 ）

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掲載種別：研究論文（学術雑誌）
Stimulation of elongation growth and xyloglucan breakdown in Arabidopsis hypocotyls under microgravity conditions in space.

Soga K, Wakabayashi K, Kamisaka S, Hoson T

Planta 215 1040 - 1046 2002年（ ISSN:320935 ）

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掲載種別：研究論文（学術雑誌）
Growth promotion and an increase in cell wall extensibility by silicon in rice and some other Poaceae seedlings.

Hossain MT, Mori R, Soga K, Wakabayashi K, Kamisaka S, Fujii S, Yamamoto R, Hoson T

J. Plant Res. 115 23 - 27 2002年（ ISSN:9189440 ）

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掲載種別：研究論文（学術雑誌）
Changes in the sugar composition and molecular mass distribution of matrix polysaccharides during cotton fiber development.

Tokumoto H, Wakabayashi K, Kamisaka S, Hoson T

Plant Cell Physiol. 43 411 - 418 2002年（ ISSN:320781 ）

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掲載種別：研究論文（学術雑誌）
Action of xyloglucan hydrolase within the native cell wall architecture and its effect on cell wall extensibility in azuki bean epicotyls.

Kaku T, Tabuchi A, Wakabayashi K, Kamisaka S, Hoson T

Plant Cell Physiol. 43 21 - 26 2002年（ ISSN:320781 ）

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掲載種別：研究論文（学術雑誌）
Purification and catalytic properties of polygalacturonase isoforms from ripe avocado (Persea americana ) fruit mesocarp.

Huber D.J.

Physiol. Plant. 113 210 - 216 2001年

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掲載種別：研究論文（学術雑誌）
Gravity regulates elongation growth of Arabidopsis hypocotyls by modifying xyloglucan metabolism.

Soga K, Hoson T and Kamisaka S

Adv.Space Res. 27 1011 - 1016 2001年

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掲載種別：研究論文（学術雑誌）
Growth inhibition of lettuce (Lactuca sativa L.) roots by alpha-amino acids, 2-amino-3-cyclopropyl-butanoic acid and 2-amino-5-chloro-4-pentenoic acid, isolated from Amanita castanopsidis Hongo.

Soga K., Hoson T., Kamisaka S., Yoshimura H., et al.

Plant Growth Regulation 33 169 - 173 2001年

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掲載種別：研究論文（学術雑誌）
Stress-relaxation analysis of submerged and air-grown rice coleoptiles: Correlations with cell wall biosynthesis and growth.

Kawamura Y., Hoson T., Yamamoto R. and Kamisaka S.

J. Plant Physiol. 156 689 - 694 2000年

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掲載種別：研究論文（学術雑誌）
Flower stalk segments of Arabidopsis thaliana ecotype Columbia lack the capacity to grow in response to exogenously applied auxin.

Soga K, Hoson T and Kamisaka S

Plant Cell Physiol. 41 1327 - 1333 2000年

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掲載種別：研究論文（学術雑誌）
Changes in the apoplastic pH are involved in regulation of xyloglucan breakdown of azuki bean epicotyls under hypergravity conditions.

Soga K, Hoson T and Kamisaka S

Plant Cell Physiol. 41 509 - 514 2000年

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掲載種別：研究論文（学術雑誌）
Hypergravity-induced increase in the apoplastic pH and its possible involvement in suppression of beta-glucan breakdown in maize seedlings.

Soga K, Hoson T and Kamisaka S

Aust. J. Plant Physiol. 27 967 - 672 2000年

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掲載種別：研究論文（学術雑誌）
Growth regulation mechanisms in higher plants under microgravity conditions - Changes in cell wall metabolism.

Hoson T., Kamisaka S., Soga K., Tabuchi A.,et al.

Biological Sciences in Space 14 75 - 96 2000年

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掲載種別：研究論文（学術雑誌）
Extensive solubilization and depolymerization of cell wall polysaccharides during avocado (Persea americana ) ripening involves concerted action of polygalacturonase and pectinmethylesterase.

Chun J.-P. and Huber D.J.

Physiol. Plant. 108 345 - 352 2000年

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掲載種別：研究論文（学術雑誌）
Auxin stimulates the synthesis but not the loosening of cell walls in isolated outer tissue of dark-grown squash (Cucurbitamaxima Duch.) hypocotyls.

Hoson T. and Sakurai N.

J. Plant Physiol. 154 197 - 202 1999年

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掲載種別：研究論文（学術雑誌）
Increased molecular mass of hemicellulosic polysaccharides is involved in growth inhibition of maize coleoptiles and mesocotyls under hypergravity conditions.

Soga K., Harada K., Hoson T. and Kamisaka S.

J. Plant Res. 112 273 - 278 1999年

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掲載種別：研究論文（学術雑誌）
Hypergravity increases the molecular mass of xyloglucans by decreasing xyloglucan-degrading activity in azuki bean epicotyls.

Soga K., Hoson T. and Kamisaka S.

Plant Cell Physiol. 40 581 - 585 1999年

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掲載種別：研究論文（学術雑誌）
Inhibition of reproductive growth of Arabidopsis in airtight vessels.

Soga K., Hoson T. and Kamisaka S.

Adv.Space Res. 23 2037 - 2040 1999年

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掲載種別：研究論文（学術雑誌）
Morphogenesis of rice and Arabidopsis seedlings in space.

Hoson T, Soga K, Mori R, Saiki M, Kamisaka S, et al.

J. Plant Res. 112 477 - 486 1999年

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掲載種別：研究論文（学術雑誌）
α-Amino acids from a mushroom, Amanita castanopsidis Hongo with growth-inhibiting activity.

Yoshimura H., Takegami K., Doe M., Yamashita T., Shibata K., et al.

Phytochemistry 52 25 - 27 1999年

　詳細を見る

掲載種別：研究論文（学術雑誌）
White light-induced sugar distribution controls growth and osmotic properties in the coleoptile and the first leaf in Zea mays seedings.

Parvez MM, Hoson T and Kamisaka S

Physiol. Plant. 102 1 - 8 1998年

　詳細を見る

掲載種別：研究論文（学術雑誌）
White light promotes the formation of diferulic acid in maize coleoptile cell walls by enhancing PAL activity.

Parvez MM, Hoson T and Kamisaka S

Physiol. Plant. 99 39 - 48 1997年

　詳細を見る

掲載種別：研究論文（学術雑誌）
Changes in amounts and molecular mass distribution of cell-wall polysaccharides of wheat (Triticum aestivum L.) coleoptiles under water stress.

Hoson T and Kamisaka S

J. Plant Physiol. 151 33 - 40 1997年

　詳細を見る

掲載種別：研究論文（学術雑誌）
Suppression of cell wall stiffening along coleoptiles of wheat (Triticum aestivum L.) seedlings grown under osmotic stress conditions.

Hoson T and Kamisaka S

J. Plant Res. 110 311 - 316 1997年

　詳細を見る

掲載種別：研究論文（学術雑誌）
Modification of chemical properties of cell wall polysaccharides in the inner tissues by white light in relation to the decrease in tissue tension in Pisum sativum epicotyls.

Miyamoto K, Mitani Y, Soga K, Ueda J, Hoson T, et al.

Physiol. Plant. 101 38 - 44 1997年

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掲載種別：研究論文（学術雑誌）
Osmotic stress suppresses the cell wall stiffening and the increase in cell wall-bound ferulic and diferulic acids in wheat coleoptiles

Hoson T and Kamisaka S

Plant Physiol. 113 967 - 973 1997年

　詳細を見る

掲載種別：研究論文（学術雑誌）
Abscisic acid suppresses the increases in cell wall-bound ferulic and diferulic acid levels in dark-grown wheat (Triticum aestivum L.) coleoptiles

Hoson T and Kamisaka S

Plant Cell Physiol. 38 811 - 817 1997年

　詳細を見る

掲載種別：研究論文（学術雑誌）
Osmotic stress-induced growth suppression of dark-grown wheat (Triticum aestivum L.) coleoptiles.

Hoson T and Kamisaka S

Plant Cell Physiol. 38 297 - 303 1997年

　詳細を見る

掲載種別：研究論文（学術雑誌）
Changes in cellular osmotic potential and mechanical properties of cell walls during light-induced inhibition of cell elongation in maize coleoptiles

Parvez MM, Hoson T and Kamisaka S

Physiol. Plant. 96 179 - 185 1996年

　詳細を見る

掲載種別：研究論文（学術雑誌）
Inhibition of the breakdown of xyloglucans in azuki bean epicotyls by concanavalin A

Hoson T and Masuda Y

Plant Cell Physiol. 36 897 - 902 1995年

　詳細を見る

掲載種別：研究論文（学術雑誌）
Unchanged molecular-weight distributions of xyloglucans in outer tissue cell walls along intact growing hypocotyls of squash (Cucurbita maxima Duch.) seedlings.

Yamaura K, Sakurai N and Kuraishi S

Plant Cell Physiol. 34 143 - 149 1993年

　詳細を見る

掲載種別：研究論文（学術雑誌）
Differential effects of auxin on molecular weight distribution of xyloglucans in cell walls of outer and inner tissues from segments of dark grown squash (Cucurbita maxima Duch.) hypocotyls

Sakurai N and Kuraishi S

Plant Physiol. 95 1070 - 1076 1991年

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掲載種別：研究論文（学術雑誌）
Effect of abscisic acid on the synthesis of cell-wall polysaccharides in segments of etiolated squash hypocotyls II. Levels of UDP-neutral sugars.

Sakurai N and Kuraishi S

Plant Cell Physiol. 32 427 - 432 1991年

　詳細を見る

掲載種別：研究論文（学術雑誌）
Sugar composition and molecular weight distribution of cell wall polyasccharides in outer and inner tissues from segments of dark grown squash (Cucurbita maxima Duch.) hypocotyls.

Sakurai N and Kuraishi S

Plant Physiol. 93 998 - 1004 1990年

　詳細を見る

掲載種別：研究論文（学術雑誌）
Effect of ABA on synthesis of cell-wall polysaccharides in segments of etiolated squash hypocotyls. I. Changes in incorporation of glucose and myo-inositol into cell-wall components.

Sakurai N and Kuraishi S

Plant Cell Physiol. 30 99 - 105 1989年

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掲載種別：研究論文（学術雑誌）
Role of the outer tissur in abscisic acid-mediated growth suppression of etiolated squash hypocotyl segments.

Sakurai N and Kuraishi S

Physiol. Plant. 75 151 - 156 1989年

　詳細を見る

掲載種別：研究論文（学術雑誌）

▼全件表示

MISC（その他記事）

Modification of cell wall architecture in gramineous plants under altered gravity conditions.

Wakabayashi K, Soga K, Hoson T

Biol Sci Space 23 137 - 142 2009年

　詳細を見る

掲載種別：記事・総説・解説・論説等（学術雑誌）
Signal perception, transduction, and response in gravity resistance. Another graviresponse in plants.

Hoson T, Saito Y, Soga K, Wakabayashi K

Adv Space Res 36 1196 - 1202 2005年

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掲載種別：記事・総説・解説・論説等（学術雑誌）
穀類(イネ科植物)の細胞壁構築における重力の作用　

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生物工学 83 571 - 573 2005年

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掲載種別：記事・総説・解説・論説等（学術雑誌）共著区分：単著
Changes in cell wall polysaccharides during fruit ripening.

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J. Plant Res. 113 231 - 237 2000年（ ISSN:09189440 ）

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掲載種別：記事・総説・解説・論説等（学術雑誌）共著区分：単著

講演・口頭発表等

エンドウ上胚軸における光による表皮・内部組織間の接着力の増強と細胞壁結合性クマル酸の蓄積国内会議

清水佑馬、若林和幸、宮本健助、曽我康一

第６５回日本植物生理学会年会 2024年03月日本植物生理学会

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会議種別：ポスター発表

開催地：神戸
スイートバジル子葉における腺毛の形成と香気性物質の生成に対する光の影響国内会議

山本はな、若林和幸、曽我康一

第１２回近畿植物学会講演会 2023年12月日本植物学会近畿支部

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会議種別：ポスター発表

開催地：吹田
エンドウ上胚軸における光照射による表皮・内部組織間の接着力の増強国内会議

清水佑馬、若林和幸、宮本健助、曽我康一

植物化学調節学会第５８回大会 2023年11月植物化学調節学会

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会議種別：ポスター発表

開催地：川崎
エンドウ上胚軸における表皮・内部組織間の接着力の測定方法の確立と接着力に対する光の影響国内会議

清水佑馬、若林和幸、宮本健助、曽我康一

日本植物学会第８７回大会 2023年09月日本植物学会

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会議種別：ポスター発表

開催地：札幌
緑化ブロッコリー芽ばえにおける遠心過重力による胚軸の成長促進国内会議

宮崎友規、東山優花、服部隆行、小林康一、若林和幸、保尊隆享、曽我康一

日本植物学会第８７回大会 2023年09月日本植物学会

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会議種別：ポスター発表

開催地：札幌
シロイヌナズナ芽生えの抗重力反応に関与する新規遺伝子PTH2 国内会議

服部隆行、曽我康一、若林和幸、保尊隆享

日本宇宙生物科学会第３７回大会 2023年09月日本宇宙生物科学会

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会議種別：口頭発表（一般）

開催地：長崎
スイートバジル子葉における精油合成に対する過重力の影響国内会議

渡部優、藤井伸治、若林和幸、曽我康一

日本宇宙生物科学会第３６回大会 2022年09月日本宇宙生物科学会

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会議種別：口頭発表（一般）

開催地：名古屋
過重力環境下での緑化ブロッコリー芽ばえの成長特性国内会議

宮崎友規、東山優花、小林康一、若林和幸、保尊隆享、曽我康一

日本宇宙生物科学会第３６回大会 2022年09月日本宇宙生物科学会

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会議種別：口頭発表（一般）

開催地：名古屋
The objectives and outlines of the Resist Tubule space experiment. 国内会議

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28th International Symposium on Space Technology andScience 2011年06月
植物の抗重力反応解明国内会議

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第27回宇宙利用シンポジウム 2011年01月
Cellular mechanisms of gravity resistance in plants. 国内会議

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日本宇宙生物科学会第24回大会 2010年09月
Outline of FERULATE experiment. 国内会議

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日本宇宙生物科学会第24回大会 2010年09月
「Resist Tubule」宇宙実験における表層微小管動態の解析方法の検討国内会議

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日本宇宙生物科学会第24回大会 2010年09月
シロイヌナズナMCA変異体の成長に対する重力の影響国内会議

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日本宇宙生物科学会第24回大会 2010年09月
Cellular basis of gravity resistance in plants. 国際会議

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38th Committee on Space Research Scientific Assembly 2010年07月
Growth and cell wall changes in stem organs under microgravity and hypergravity conditions. 国際会議

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38th Committee on Space Research Scientific Assembly 2010年07月
エチレンによるアズキ上胚軸の表層微小管の配向変化とgamma-チューブリン複合体およびカタニンの発現増加国内会議

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第51回日本植物生理学会年会 2010年03月
平成21年度WG「高等植物の生活環」活動報告国内会議

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第26回宇宙利用シンポジウム 2010年01月
植物の抗重力反応解明国内会議

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第26回宇宙利用シンポジウム 2010年01月
Displacemant by gravity of the nucleus in epidermal cells of azuki bean epicotyls.　国際会議

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日本宇宙生物科学会第23回大会 2009年10月
Growth and cell wall properties in hypocotyls of Arabidopsis tua6 mutant under microgravity conditions in space. 国内会議

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日本宇宙生物科学会第23回大会　 2009年10月
Development of cultivation vessel and determination of growth conditions of Arabidopsis for the Resist Tubule space experiment.　国内会議

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日本宇宙生物科学会第23回大会 2009年10月
Mechanoreceptors are involved in gravity-induced modifications to development in hypocotyls of Arabidopsis tubulin mutants.　国際会議

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日本宇宙生物科学会第23回大会 2009年10月
Effects of hypergravity on osmoregulation in azuki bean epicotyls.　国内会議

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日本宇宙生物科学会第23回大会 2009年10月
シロイヌナズナの純系T-DNA挿入ラインにおける細胞壁物性の変化国内会議

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日本植物学会第73回大会 2009年09月

▼全件表示

担当授業科目

専門生物学実験1

2024年度週間授業大学
植物生理学1

2024年度週間授業大学
生物学の潮流

2024年度週間授業大学
生物学特別研究2A

2024年度集中講義大学院
生物学特別研究1A

2024年度集中講義大学院
生体機能生物学演習2A

2024年度集中講義大学院
生体機能生物学演習1A

2024年度集中講義大学院
生体機能生物学特論C

2024年度週間授業大学院
生体機能生物学特論C

2024年度週間授業大学院
植物の科学

2024年度週間授業大学院
生物学特別研究5A

2024年度集中講義大学院
生物学特別研究4A

2024年度集中講義大学院
生物学特別研究3A

2024年度集中講義大学院
生物学実験A

2024年度週間授業大学院
後期特別研究

2024年度集中講義大学院
生物学実験Ｓ

2024年度週間授業大学
専門生物学実験Ｂ

2023年度週間授業大学
生物学の潮流

2023年度週間授業大学
生命と進化

2023年度週間授業大学
植物の科学

2023年度週間授業大学
専門生物学演習

2023年度集中講義大学
生物学実験A

2023年度週間授業大学
植物生理学1

2023年度週間授業大学
生物学実験Ｓ

2023年度週間授業大学
特別研究

2023年度集中講義大学
生体機能生物学特論C

2023年度週間授業大学院
生体機能生物学演習1B

2023年度集中講義大学院
生体機能生物学演習2B

2023年度集中講義大学院
生体機能生物学ゼミナール

2023年度集中講義大学院
生体機能生物学演習1A

2023年度集中講義大学院
生体機能生物学演習2A

2023年度集中講義大学院
生物学特別研究1A

2023年度集中講義大学院
生物学特別研究2A

2023年度集中講義大学院
生物学特別研究1B

2023年度集中講義大学院
生物学特別研究2B

2023年度集中講義大学院
特別研究（生物）

2022年度集中講義大学
後期特別研究

2011年度
生命と進化

2011年度大学
生物学実験A

2011年度大学
実験で知る自然の世界

2011年度大学
植物生理学

2011年度大学
専門生物学実験B

2011年度大学
専門生物学演習

2011年度大学
特別研究

2011年度大学
植物機能学特論II

2011年度
生体機能生物学演習

2011年度
前期特別研究

2011年度
生体機能生物学ゼミナール

2011年度

▼全件表示

学外での担当授業科目

２０２２年度理学セミナー

2022年10月
機関名：日本女子大学

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科目区分：大学院専門科目国名：日本国

所属院生等の論文発表集計

2023年度

所属大学院生発表数：2件
2022年度

所属大学院生発表数：1件

論文・研究指導集計

2023年度

卒業論文指導数：0名

博士前期課程学生指導数：3名

修士論文審査数（主査）：1件
2022年度

卒業論文指導数：3名

博士前期課程学生指導数：3名博士後期課程学生指導数：1名

修士論文審査数（副査）：2件

博士論文審査数（副査）：1件

社会貢献活動　⇒ 社会貢献実績一覧へ

宇宙と生命の神秘へ遭遇する科

役割：講師

種別：セミナー・ワークショップ

大阪府高齢者大学校 2023年09月
宇宙で植物を育てる

役割：講師

種別：セミナー・ワークショップ

大阪公立大学 2022 公大授業 2022年04月

担当・職階

担当・職階

取得学位

取得学位

研究分野

研究分野

研究歴

研究歴

所属学協会

所属学協会

職務経歴（学外）

職務経歴（学外）

学歴

学歴

論文

論文

MISC（その他記事）

MISC（その他記事）

講演・口頭発表等

講演・口頭発表等

担当授業科目

担当授業科目

学外での担当授業科目

学外での担当授業科目

所属院生等の論文発表集計

所属院生等の論文発表集計

論文・研究指導集計

論文・研究指導集計

社会貢献活動 ⇒ 社会貢献実績一覧へ

社会貢献活動 ⇒ 社会貢献実績一覧へ

社会貢献活動　⇒ 社会貢献実績一覧へ

社会貢献活動　⇒ 社会貢献実績一覧へ