Updated on 2024/07/12

写真a

 
WADA Takayuki
 
Organization
Graduate School of Human Life and Ecology Division of Human Life and Ecology Professor
School of Human Life and Ecology Department of Nutrition
Title
Professor
Affiliation
Institute of Human Life and Ecology
Profile
1993 京都大学理学部　入学 1997 京都大学大学院理学研究科博士課程（前期）入学 1999 同　修了 2006 大阪市立大学大学院医学研究科　博士（医学）取得 1999-2009 大阪市立環境科学研究所　研究員 2009-2012 同　研究主任 2013-2017 長崎大学熱帯医学研究所　国際保健学分野　助教 2017-2020 同　准教授 2020- 大阪市立大学大学院生活科学研究科　教授

Position

  • Graduate School of Human Life and Ecology Division of Human Life and Ecology 

    Professor  2022.04 - Now

  • School of Human Life and Ecology Department of Nutrition 

    Professor  2022.04 - Now

Degree

  • 博士（医学） （ Osaka City University ）

Research Areas

  • Life Science / Hygiene and public health (non-laboratory)  / molecular epidemiology of infectious diseases

  • Life Science / Hygiene and public health (laboratory)  / molecular epidemiology of infectious diseases

  • Life Science / Bacteriology  / Genome microbiology

  • Life Science / Hygiene and public health (laboratory)

  • Life Science / Hygiene and public health (non-laboratory)

  • Life Science / Bacteriology

  • Life Science / Veterinary medical science

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Professional Memberships

  • 日本公衆衛生学会

      Domestic

  • 日本結核・非結核性抗酸菌症学会

      Domestic

  • JAPANESE SOCIETY FOR BACTERIOLOGY

Awards

  • 研究奨励賞

    和田崇之

    2014.05   日本結核病学会  

Job Career (off-campus)

  • Osaka Metropolitan University   Graduate School of Human Life and Ecology

    2020.04 - Now

  • Nagasaki University   Institute of Tropical Medicine

    2017.05 - Now

  • 長崎大学熱帯医学研究所   准教授

    2017.05 - 2020.03

  • 長崎大学熱帯医学研究所   助教

    2013.01 - 2017.04

  • Nagasaki University   Institute of Tropical Medicine

    2013.01 - 2017.04

  • 大阪市立環境科学研究所   研究主任

    2009.04 - 2012.12

  • 大阪市立環境科学研究所   研究員

    1999.04 - 2009.03

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Education

  • Kyoto University   Master's Course   Graduated/Completed

    1997.04 - 1999.03

  • Kyoto University     Graduated/Completed

    1993.04 - 1997.03

Papers

  • Pathogenicity of enterotoxigenic Escherichia coli in Caenorhabditis elegans as an alternative model host.

    Anri Matsuda, Takashi Ishida, Yoshihiko Tanimoto, Takayuki Wada, Eriko Kage-Nakadai

    Bioscience, biotechnology, and biochemistry   2023.12

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Enterotoxigenic Escherichia coli (ETEC), one of the diarrheagenic E. coli, is the most common cause of diarrhea in developing country, and in travelers to those areas. In this study, Caenorhabditis elegans was used as an alternative model host to evaluate ETEC infections. The ETEC strain ETEC1, which was isolated from a patient with diarrhea, possessed enterotoxins STh, LT1, and EAST1 and colonization factors CS2 and CS3. Live ETEC1 shortened the lifespan and body size of C. elegans in association with increased expression of enterotoxin genes and intestinal colonization. In contrast, heat-killed ETEC1 did not affect the lifespan of C. elegans. C. elegans infected with ETEC1 showed upregulated expression of genes related to insulin-like peptides and host defense responses. These results suggest that ETEC1 exhibits pathogenicity through intestinal colonization and enterotoxin production in C. elegans. This system is useful as an ETEC infection model.

    DOI： 10.1093/bbb/zbad185

    PubMed

  • Improvement of Locomotion Caused by Lactococcus lactis subsp. lactis in the Model Organism Caenorhabditis elegans.

    Mohammad Shaokat Ali, Shamima Ahmed, Shino Takeuchi, Takayuki Wada, Eriko Kage-Nakadai

    Nutrients   15 ( 20 )   2023.10

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Lactococcus lactis subsp. lactis exhibits probiotic properties in humans. Considering that Caenorhabditis elegans can be used to study the effects of microorganisms on animal behavior, owing to its simple nervous system, we assessed the impacts of two strains of Lactococcus lactis subsp. Lactis-a non-nisin-producing strain, NBRC 100933 (LL100933), and a nisin-producing strain, NBRC 12007 (LL12007)-on the lifespan, locomotion, reproductive capacity of, and lipid accumulation in, C. elegans. The lifespan of adult C. elegans fed a mixture (1:1) of Escherichia coli OP50 and LL100933 or LL12007 did not show a significant increase compared to that of the group fed a standard diet of E. coli OP50. However, the nematodes fed Lactococcus strains showed notable enhancement in their locomotion at all of the tested ages. Further, the beneficial effects of LL100933 and LL12007 were observed in the daf-16 mutants, but not in the skn-1 and pmk-1 mutants. The lipid accumulation in the worms of the Lactococcus-fed group was lower than that in the control group at all experimental ages. Overall, LL100933 and LL12007 enhance the locomotor behavior of C. elegans, likely by modulating the PMK-1/p38 MAPK and SKN-1/Nrf2 transcription factors.

    DOI： 10.3390/nu15204482

    PubMed

  • Retrospective identification of pathogenic mycobacterial species in fish: <i>Mycobacterium pseudoshottsii</i> YM-3, isolated from a yellowtail fish in 1986 in Kochi, Japan Reviewed

    Masayuki Imajoh, Shiomi Yoshida, Lisa Nonaka, Yukari Fukushima, Chie Nakajima, Yasuhiko Suzuki, Takayuki Wada

    Microbiology Resource Announcements   2023.09（ eISSN:2576-098X ）

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    Publishing type：Research paper (scientific journal)  

    ABSTRACT

    The complete genome sequence of mycobacterial strain YM-3, isolated from cultured yellowtail in 1986, was determined. The strain was Mycobacterium pseudoshottsii , a closely related subspecies of Mycobacterium marinum , so the strain was isolated earlier than the first report of the subspecies in 2005.

    DOI： 10.1128/mra.00498-23

  • Impacts of Bacillus subtilis var. natto on the lifespan and stress resistance of Caenorhabditis elegans.

    Nao Teramoto, Kanae Sato, Takayuki Wada, Yoshikazu Nishikawa, Eriko Kage-Nakadai

    Journal of applied microbiology   134 ( 4 )   2023.04

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    AIM: Bacillus subtilis var. natto is used in the production of natto, a typical Japanese fermented soybean food. Although the probiotic attributes and health-related effects of B. subtilis var. natto have been reported, the effect on longevity remains unknown. In the present study, the effects of B. subtilis var. natto strains on lifespan extension and the molecular mechanisms governing the prolongevity were examined using Caenorhabditis elegans as a model animal. METHODS AND RESULTS: Synchronized 3-day-old (young adult) worms were fed Escherichia coli OP50 (control) or a subcloned isolate of B. subtilis var. natto Miyagino strain (MI-OMU01) and subjected to lifespan, survival against pathogens and abiotic stress resistance assays. Notably, the lifespan of worms fed MI-OMU01 was significantly longer than that of the animals fed OP50. Moreover, MI-OMU01 increased the resistance of C. elegans to several stressors, including UV irradiation, H2O2, and Cu2+. CONCLUSIONS: Genetic and gene expression analyses using mutant animals suggested that MI-OMU01 extended the lifespan of worms in TIR-1/SARM, p38 MAPK, and insulin/IGF-1 signaling pathway-dependent manners.

    DOI： 10.1093/jambio/lxad082

    PubMed

  • Social dilemma in the excess use of antimicrobials incurring antimicrobial resistance Reviewed

    Hiromu Ito, Takayuki Wada, Genki Ichinose, Jun Tanimoto, Jin Yoshimura, Taro Yamamoto, Satoru Morita

    Scientific Reports   12   21084   2022.12

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    DOI： 10.1038/s41598-022-25632-1

  • Oral cleaning habits and the copy number of periodontal bacteria in pregnant women and its correlation with birth outcomes: an epidemiological study in Mibilizi, Rwanda Reviewed

    Hiroaki Arima, Akintije Simba Calliope, Hideki Fukuda, Theoneste Nzaramba, Marie Goretti Mukakarake, Takayuki Wada, Takashi Yorifuji, Leon Mutesa, Taro Yamamoto

    BMC Oral Health   22 ( 1 )   2022.09（ eISSN:1472-6831 ）

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    Publishing type：Research paper (scientific journal)  

    Abstract

    Background

    Since 1996, many studies have reported that periodontal disease during pregnancy may be a risk factor for preterm birth and low birth weight; however, in Africa, periodontal disease is considered a non-high-priority disease. In addition, there are few dental facilities in rural Rwanda; thus, the oral condition of pregnant women has not been investigated. The objective of this study was to assess the tooth brushing habits of pregnant women in rural Rwanda and evaluate whether periodontal bacteria in the oral cavity of pregnant women are related to birth outcomes or oral cleaning habits.

    Methods

    A questionnaire survey and saliva collection were conducted for pregnant women in the catchment area population of Mibilizi Hospital located in the western part of Rwanda. Real-time PCR was performed to quantitatively detect total bacteria and 4 species of periodontal bacteria. The relationship of the copy number of each bacterium and birth outcomes or oral cleaning habits was statistically analyzed.

    Results

    Among the participants, high copy numbers of total bacteria, Tannerella forsythia, and Treponema denticola were correlated with lower birth weight (p = 0.0032, 0.0212, 0.0288, respectively). The sex ratio at birth was higher in women who had high copy numbers of Porphyromonas gingivalis and T. denticola during pregnancy (p = 0.0268, 0.0043). Furthermore, regarding the correlation between oral cleaning habits and the amount of bacteria, the more frequently teeth were brushed, the lower the level of P. gingivalis (p = 0.0061); the more frequently the brush was replaced, the lower the levels of P. gingivalis and T. forsythia (p = 0.0153, 0.0029).

    Conclusions

    This study suggested that improving tooth brushing habits may reduce the risk of periodontal disease among pregnant women in rural Rwanda. It also indicated that the amount of bacteria is associated with various birth outcomes according to the bacterial species. Both access to dental clinics and the oral cleaning habits of pregnant women should be important considerations in efforts to alleviate reproductive-related outcomes in rural Africa.

    DOI： 10.1186/s12903-022-02443-4

    Other URL： https://link.springer.com/article/10.1186/s12903-022-02443-4/fulltext.html

  • Comparative genomic analysis of Leptospira spp. isolated from Rattus norvegicus in Indonesia Reviewed

    Nobuo Koizumi, Masatomo Morita, Harimurti Nuradji, Susanti, Susan M. Noor, N.L.P. Indi Dharmayanti, Patriantariksina Randusari, Jung-Jung Mu, Rontgene M. Solante, Nobuo Saito, Koya Ariyoshi, Hoang Thi Thu Ha, Takayuki Wada, Yukihiro Akeda, Kozue Miura

    Infection, Genetics and Evolution   102   105306 - 105306   2022.08（ ISSN:1567-1348 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.meegid.2022.105306

  • Unique genomic sequences in novel <i>Mycobacterium avium</i> subsp. <i>hominissuis</i> lineage enabled fine scale tracing the transmission route during pig movement

    Tetsuya Komatsu, Kenji Ohya, Atsushi Ota, Yukiko Nishiuchi, Hirokazu Yano, Kayoko Matsuo, Justice Opare Odoi, Shota Suganuma, Kotaro Sawai, Akemi Hasebe, Tetsuo Asai, Tokuma Yanai, Hideto Fukushi, Takayuki Wada, Shiomi Yoshida, Toshihiro Ito, Kentaro Arikawa, Mikihiko Kawai, Manabu Ato, Anthony D. Baughn, Tomotada Iwamoto, Fumito Maruyama

    2022.04

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    Publishing type：Research paper (scientific journal)  

    Abstract

    Mycobacterium avium subsp. hominissuis (MAH) is one of the most prevalent mycobacteria causing non-tuberculous mycobacterial (NTM) disease in human and animals. Of note, MAH is a major cause of mycobacterial granulomatous mesenteric lymphadenitis outbreaks in pig populations. To determine the precise source of infection of MAH in a pig farm and to clarify the epidemiological relationship among pig and human MAH lineages, we collected 50 MAH isolates from pigs reared in Japan and determined draft genome sequences of 30 isolates. A variable number of tandem repeat (VNTR) analysis revealed that most pig MAH isolates in Japan were closely related to North American, European and Russian human isolates but not to those from East Asian human. Historical recombination analysis revealed that most pig isolates could be classified into SC2/4 and SC3. To our surprise, four isolates belonged to a new lineage (SC5) in the global MAH population. Members of SC5 had few footprints of inter-lineage recombination in the genome, and carried 80 unique genes, most of which were located on lineage specific-genomic islands. As its unique genetic features, we were able to trace the putative transmission route via their host pigs. Together, these results reveal the diversity in the pig MAH population and clarify the possibility of species-specificity in MAH in addition to local adaptation. Moreover, our study also clarified the difference of source of infection and the vector of transmission between humans and pigs and provides a clear countermeasure to prevent MAH spreading in pigs.

    Importance

    Mycobacterium avium subsp. hominissuis (MAH) is a dominant cause of non- tuberculous mycobacterial (NTM) disease in human and animals. In this study, in order to clarify the epidemiological relatedness between MAH isolates from human and pigs, and the MAH transmission route among pigs, we conducted molecular and genomic- based epidemiological research. The pig isolates were genetically distant from those of humans in Japan, however, they were closely related to human isolates from North America, Europe and Russia, suggesting a proportion of pig MAH isolates are imported from abroad. Importantly, we identified a novel lineage of MAH that was distributed in a specific area in Japan. Epidemiological survey and its unique genomic features allowed us to trace putative transmission routes via pig movement. Our study provides critical information for understanding the diversity of MAH population and the possibility of species-specificity in MAH. Our study will be useful for countermeasures of pig NTM disease.

    DOI： 10.1101/2022.04.04.487006

  • High production of egc2-related staphylococcal enterotoxins caused a food poisoning outbreak.

    Kaoru Umeda, Hisaya K Ono, Takayuki Wada, Daisuke Motooka, Shota Nakamura, Hiromi Nakamura, Dong-Liang Hu

    International journal of food microbiology   357   109366 - 109366   2021.11

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    Staphylococcal enterotoxins (SEs) produced by enterotoxigenic Staphylococcus aureus in food cause staphylococcal food poisoning. We recently reported a foodborne outbreak due to S. aureus harboring new SE/SE-like (SEl) genes (seg, sei, sem, sen, seo, and selu) related to enterotoxin gene cluster (egc) 2 as with other research groups. However, the pathogenicity of SEs production remains unclear. Therefore, we herein investigated egc2-related SEs production from S. aureus isolates and leftover food items during a foodborne outbreak using a sandwich enzyme-linked immunosorbent assay suitable for the quantification of SEs. S. aureus isolates produced markedly high levels of egc2-related SEs, and the leftover food item "Sushi" contained SEs over the toxin dose that causes food poisoning symptoms. A representative isolate was subjected to whole-genome sequencing. The isolate was homologous with previously reported ST45 strains, particularly the unique genomic island νSaβ structure mostly consisting of egc2. The present study indicates that egc2-related SEs are food poisoning causative agents based on high SE production levels within an actual foodborne outbreak.

    DOI： 10.1016/j.ijfoodmicro.2021.109366

    PubMed

  • Attempt of thyX gene silencing and construction of a thyX deleted clone in a Mycobacterium bovis BCG.

    Yuki Arimura, Yusuke Minato, Takayuki Wada, Masaaki Nakayama, Ayako Ryumon, Nao Hirata, Chie Nakajima, Yasuhiko Suzuki, Manabu Ato, Kazuo Kobayashi, Naoko Ohara, Seiji Iida, Naoya Ohara

    Microbiology and immunology   2021.09

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    Mycobacterium tuberculosis, the causative agent of tuberculosis, possess flavin-dependent thymidylate synthase, ThyX. Since thyX is absent in humans and was shown to be essential for M. tuberculosis normal growth, ThyX is thought to be an attractive novel TB drug target. In this study, we assessed thyX essentiality in Mycobacterium bovis BCG strains using CRISPR interference based gene silencing and found that thyX is not essential in an M. bovis BCG Tokyo derivative strain. We successfully constructed a thyX deletion mutant strain from that strain, which reinforces the non-essentiality of thyX under a certain genetic background. This article is protected by copyright. All rights reserved.

    DOI： 10.1111/1348-0421.12944

    PubMed

  • Genomic features of Mycobacterium avium subsp. hominissuis isolated from pigs in Japan

    Tetsuya Komatsu, Kenji Ohya, Atsushi Ota, Yukiko Nishiuchi, Hirokazu Yano, Kayoko Matsuo, Justice Opare Odoi, Shota Suganuma, Kotaro Sawai, Akemi Hasebe, Tetsuo Asai, Tokuma Yanai, Hideto Fukushi, Takayuki Wada, Shiomi Yoshida, Toshihiro Ito, Kentaro Arikawa, Mikihiko Kawai, Manabu Ato, Anthony D Baughn, Tomotada Iwamoto, Fumito Maruyama

    2021.06

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    <title>Abstract</title><italic>Mycobacterium avium</italic> subsp. <italic>hominissuis</italic> (MAH) is one of the most important agents causing non-tuberculosis mycobacterial infection in humans and pigs. Genome analysis on MAH of human isolates has been proceeding, however, those of pigs are limited despite its potential source of infection to human. In the current study, we obtained 30 draft genome sequences of MAH of pigs reared in Japan. The 30 draft genomes consisted of 4,848,678 – 5,620,788 bp length, 4,652 – 5,388 coding genes and 46 – 75 (Med: 47) tRNAs. All isolates had restriction modification associated genes and 185 – 222 predicted virulence genes. Two isolates had tRNA arrays and one isolate had a clustered regularly interspaced short palindromic repeat (CRISPR) region. Our results will be useful for evaluation of the ecology of MAH by providing a foundation for genome-based epidemiological studies.

    DOI： 10.1101/2021.06.15.447579

  • Rat trade and leptospirosis: Molecular epidemiology of Leptospira species in rats exported from Cambodia to Vietnam.

    Nobuo Koizumi, Masatomo Morita, Vutha Pheng, Chinda Wann, Hiroaki Masuoka, Yukiko Higa, Takayuki Wada, Kazuhiro Hirayama, Makoto Ohnishi, Kozue Miura

    Transboundary and emerging diseases   2021.03

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    Rats are an important maintenance host of Leptospira spp., the causative agents of leptospirosis, a zoonotic disease with worldwide distribution. Rats are traded as food in the Mekong Delta, where Cambodia exports tons of rats to Vietnam. Handling wild rats is a potential health risk, but the information on Leptospira spp. carried by rats traded in the region remains limited. In this study, we investigated the carriage of Leptospira spp. in rats exported from Cambodia to Vietnam using bacterial culture, nested PCR and DNA sequencing. Isolates were then assessed using serological analysis and whole-genome sequencing (WGS), and Leptospira DNA detected in rat kidney tissues was also analysed by multilocus sequence typing (MLST). Seventy-two rats (2 Bandicota indica, 57 Rattus argentiventer, 11 R. losea, 1 R. norvegicus and 1 R. rattus) were subjected to bacterial culture, and three L. borgpetersenii serogroup Javanica isolates were obtained from R. argentiventer (5.3%). WGS revealed that although Cambodian isolates were genetically related to L. borgpetersenii serogroup Javanica strains widely distributed in East and South-East Asian countries, they formed a different cluster from other strains. In addition to the three L. borgpetersenii sequences, the flaB sequence of L. interrogans was detected in 18 R. argentiventer and 7 R. losea kidney tissue samples (38.9%) using nested PCR followed by DNA sequencing. The L. interrogans flaB-positive samples were further analysed by MLST, revealing that seven housekeeping genes (glmU, pntA, sucA, tpiA, pfkB, mreA and caiB) contained novel sequences with distinct lineages from other sequence types. This study revealed a high prevalence of Leptospira spp. among rats exported from Cambodia to Vietnam, indicating a potential risk to people engaging in rat trade and demonstrating that a fastidious L. interrogans strain circulates among Cambodian rats.

    DOI： 10.1111/tbed.14077

    PubMed

  • Intensive Treatment of a Captive Bornean Elephant (Elephas maximus borneensis) Infected with Mycobacterium caprae in Japan Reviewed International coauthorship

    Satomi Suga, Yasuhiko Mukai, Satoshi Ishikawa, Shiomi Yoshida, Sarad Paudel, Takayuki Wada

    Journal of Zoo and Wildlife Medicine   51 ( 4 )   2021.01（ ISSN:1042-7260 ）

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    Kind of work：Joint Work  

    DOI： 10.1638/2019-0152

  • Unique hemoglobin dynamics in female Tibetan highlanders Reviewed International coauthorship

    Hiroaki Arima, Masayuki Nakano, Sweta Koirala, Hiromu Ito, Basu Dev Pandey, Kishor Pandey, Takayuki Wada, Taro Yamamoto

    Tropical Medicine and Health   49 ( 1 )   2021.01

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    Kind of work：Joint Work  

    <title>Abstract</title><sec>
    <title>Background</title>
    Tibetan highlanders have adapted to hypoxic environments through the development of unique mechanisms that suppress an increase in hemoglobin (Hb) concentration even in high-altitude areas. Hb concentrations generally decrease with increasing age. However, in the highlands, chronic altitude sickness is known to occur in the elderly population. To investigate how aging in a hypoxic environment affects Hb levels in Tibetan highlanders, we focused on the Mustang people, who live above 3500 m. We tried to clarify the pure relationship between aging and Hb levels in a hypoxic environment.
    </sec><sec>
    <title>Results</title>
    We found that the Hb concentration increased with increasing age in females but not in males. Multivariate analysis showed that age, pulse pressure, the poverty index, and vascular diameter were strongly correlated with the Hb concentration.
    </sec><sec>
    <title>Conclusions</title>
    We found unique Hb dynamics among female Tibetan highlanders. As seen in these Hb dynamics, there may be sex-based differences in the adaptive mechanism in Tibetan highlanders.
    </sec>

    DOI： 10.1186/s41182-020-00289-6

    Other URL： http://link.springer.com/article/10.1186/s41182-020-00289-6/fulltext.html

  • Seroepidemiological survey on pigs and cattle for novel K88 (F4)-like colonization factor detected in human enterotoxigenic Escherichia coli Reviewed

    Yoshihiko Tanimoto, Miyoko Inoue, Kana Komatsu, Atsuyuki Odani, Takayuki Wada, Eriko Kage-Nakadai, Yoshikazu Nishikawa

    Epidemiology and Infection   2021（ ISSN:0950-2688 ）

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    Publishing type：Research paper (scientific journal)  

    Enterotoxigenic Escherichia coli (ETEC) strains that express various fimbrial or nonfimbrial colonization factors and enterotoxins are critical causes of diarrheal diseases. Human ETEC serotype O169:H41 (O169) has been a representative of epidemic ETEC worldwide; the organism shows massive adherence to HEp-2 cells similar to enteroaggregative E. coli. Previously, we determined the complete sequence of the unstable virulence plasmid, pEntYN10. The plasmid included a unique set of genes encoding a novel colonization factor (CF) resembling K88 (F4) of porcine ETEC, in addition to CS6, a well-known representative CF of human ETEC, and another novel CF similar to CS8 (CFA/III) of human ETEC. In the present study, we focused on K88-like CF (hereafter, K88O169) that may allow the organisms to infect domestic livestock like original K88-harboring strains that can cause diarrhea in piglets. Samples were tested for antibodies against recombinant proteins of possible paralogous adhesins, FaeG1 and FaeG2, from K88O169 and the FaeG of typical K88 (F4). The seroepidemiological study using recombinant antigens (two paralogs FaeG1 and FaeG2 from K88Oi69) showed reactivity of porcine (18.0%) and bovine (17.1%) sera to K88O169 FaeG1 and/or FaeG2 antigens on indirect ELISA tests. These results suggest that E. coli with K88O169 adhesin can infect various hosts, including pigs and cattle. This is the first report of domestic livestock having antibodies to K88O169 of human ETEC. Although human ETEC had been thought to be distinguished from those of domestic animals based on colonization factors, zoonotic strains may conceal themselves among human ETEC organisms. The concept of One Health should be adopted to intervene in ETEC infections among animals and humans.

    DOI： 10.1017/S0950268821002697

  • Emergence of the Novel Aminoglycoside Acetyltransferase Variant aac(6′)-Ib-D179Y and Acquisition of Colistin Heteroresistance in Carbapenem-Resistant Klebsiella pneumoniae Due to a Disrupting Mutation in the DNA Repair Enzyme MutS Reviewed

    Toyotaka Sato, Takayuki Wada, Suguru Nishijima, Yukari Fukushima, Chie Nakajima, Yasuhiko Suzuki, Satoshi Takahashi, Shin-ichi Yokota

    mBio   11 ( 6 )   2020.12

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    Kind of work：Joint Work  

    The emergence of multidrug resistance in pathogens such as <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> is of great clinical concern. Antimicrobial resistance sometimes arises during the course of an infection.

    DOI： 10.1128/mbio.01954-20

  • Antenatal Care Visits and Adverse Pregnancy Outcomes at a Hospital in Rural Western Province, Rwanda Reviewed International coauthorship

    Calliope Simba Akintije, Takashi Yorifuji, Takayuki Wada, Marie Goret, Mukakarake, Leon Mutesa, Taro Yamamoto

    Acta Medica Okayama   74 ( 6 )   495 - 503   2020.12

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    Kind of work：Joint Work  

  • Comparative genomic analysis of Leptospira borgpetersenii serogroup Javanica isolated from Rattus species in Southern Japan, Philippines, and Taiwan Reviewed International coauthorship

    Nobuo Koizumi, Takayuki Wada, Masatomo Morita, Jung-Jung Mu, Makoto Ohnishi

    Infection, Genetics and Evolution   85   104447 - 104447   2020.11（ ISSN:1567-1348 ）

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    Kind of work：Joint Work  

    DOI： 10.1016/j.meegid.2020.104447

  • Non-compulsory measures sufficiently reduced human mobility in Tokyo during the COVID-19 epidemic Reviewed International coauthorship

    Takahiro Yabe, Kota Tsubouchi, Naoya Fujiwara, Takayuki Wada, Yoshihide Sekimoto, Satish V. Ukkusuri

    Scientific Reports   10 ( 1 )   2020.10

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    Kind of work：Joint Work  

    <title>Abstract</title>
    While large scale mobility data has become a popular tool to monitor the mobility patterns during the COVID-19 pandemic, the impacts of non-compulsory measures in Tokyo, Japan on human mobility patterns has been under-studied. Here, we analyze the temporal changes in human mobility behavior, social contact rates, and their correlations with the transmissibility of COVID-19, using mobility data collected from more than 200K anonymized mobile phone users in Tokyo. The analysis concludes that by April 15th (1 week into state of emergency), human mobility behavior decreased by around 50%, resulting in a 70% reduction of social contacts in Tokyo, showing the strong relationships with non-compulsory measures. Furthermore, the reduction in data-driven human mobility metrics showed correlation with the decrease in estimated effective reproduction number of COVID-19 in Tokyo. Such empirical insights could inform policy makers on deciding sufficient levels of mobility reduction to contain the disease.

    DOI： 10.1038/s41598-020-75033-5

    Other URL： http://www.nature.com/articles/s41598-020-75033-5

  • Emergence of vancomycin- and teicoplanin-resistant Enterococcus faecium via vanD5-harbouring large genomic island Reviewed

    Toyotaka Sato, Takayuki Wada, Masaaki Shinagawa, Yukari Fukushima, Chie Nakajima, Yasuhiko Suzuki, Satoshi Takahashi, Shin-ichi Yokota

    Journal of Antimicrobial Chemotherapy   75 ( 9 )   2411 - 2415   2020.09（ ISSN:0305-7453 ）

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    Kind of work：Joint Work  

    <title>Abstract</title>
    <sec>
    <title>Background</title>
    Treatment of VRE is of clinical concern. While certain numbers of vanD-type VRE have been isolated, only two vanD5-harbouring Enterococcus faecium isolates have been reported in Canada and Japan.
    </sec>
    <sec>
    <title>Methods</title>
    We report the isolation of vanD5-type E. faecium and the first ever determination of the whole-genome sequence to investigate the possible mechanisms of the acquisition of the vanD5 gene cluster in E. faecium.
    </sec>
    <sec>
    <title>Results</title>
    Two vanD5-harbouring vancomycin-resistant E. faecium were isolated from the skin (SMVRE19) and faeces (SMVRE20) of a patient with a skin ulcer in Japan. The isolates exhibited vancomycin and teicoplanin MIC values of 128 mg/L, whilst the previous isolates of vanD5-harbouring E. faecium were only resistant to vancomycin. SMVRE19 and SMVRE20 were clones related to ST18, which is also seen in vanA- and vanB-type VRE. These isolates harboured an insertion element, ISEfm1, in the ddl gene, similar to a previously described teicoplanin-resistant vanD3-type E. faecium. The vanD5 gene cluster was integrated into the SMVRE20 chromosome as a part of a large genomic island (approximately 127 kb), similar to other recently spreading vanD variants in the Netherlands. The genomic island shared the greatest similarity with a part of the Blautia coccoides genome sequence, except for the region surrounding the vanD gene cluster.
    </sec>
    <sec>
    <title>Conclusions</title>
    This study reports that emergence of vancomycin- and teicoplanin-resistant vanD5-type E. faecium occurred via acquisition of the vanD5 cluster and ISEfm1 insertion into ddl. Considering the genetic similarity between the various VRE strains, the current study should serve as a warning against the spread of vanD5-type VRE.
    </sec>

    DOI： 10.1093/jac/dkaa220

  • Isolation and Antimicrobial Susceptibilities of Nontuberculous Mycobacteria from Wildlife in Japan Reviewed

    Justice Opare Odoi, Kenji Ohya, Junji Moribe, Yasuhiro Takashima, Kotaro Sawai, Kyoichiro Taguchi, Hideto Fukushi, Takayuki Wada, Shiomi Yoshida, Tetsuo Asai

    Journal of wildlife diseases   2020.05

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    Kind of work：Joint Work  

    Nontuberculous mycobacteria (NTM) are opportunistic pathogens of humans and animals and are transmitted among the environment, wildlife, livestock, and humans. The aim of this study was to investigate the rate of isolation and antimicrobial susceptibility of NTM in wildlife. In total, 178 samples of feces (n=131) and tissues (n=47) were collected from 11 wildlife species in Gifu Prefecture and Mie Prefecture, Japan, between June 2016 and October 2018. We isolated NTM from 15.3% (20/131) of fecal samples using Ogawa medium, and isolates were identified by sequencing the rpoB and hsp65 genes. The rpoB sequences were compared with those from other strains of human and environmental origin. The NTM isolates were obtained from sika deer (Cervus nippon), wild boar (Sus scrofa), Japanese monkey (Macaca fuscata), raccoon dog (Nyctereutes procyonoides), masked palm civet (Paguma larvata), and Japanese weasel (Mustela itatsi) and were classified as rapidly growing mycobacteria (RGM) and slowly growing mycobacteria (SGM). The 12 RGM identified were Mycolicibacterium peregrinum (n=5), Mycolicibacterium fortuitum (n=3), Mycolicibacterium septicum (n=3), and Mycolicibacterium thermoresistibile (n=1), and the eight SGM were Mycobacterium paraense (n=4), Mycolicibacter arupensis (n=2), Mycolicibacter virginiensis (n=1), and Mycobacterium nebraskense (n=1). The NTM from wildlife showed ≥99% similarity with strains from different sources including humans. The RGM were susceptible to the antimicrobial agents tested except for M. fortuitum, which was resistant to azithromycin and clarithromycin. The SGM showed multiple drug resistance qualities but were susceptible to amikacin, clarithromycin, and rifabutin. These results indicate that wildlife may be reservoir hosts of NTM in Japan. The presence of antimicrobial-resistant NTM in wildlife suggests that the trends of NTM antimicrobial susceptibility in wildlife should be monitored.

    DOI： 10.7589/2019-10-261

    PubMed

  • 感染抑制政策の最終規模への影響

    藤原 直哉, 翁長 朝功, 和田 崇之, 合原 一幸

    生産研究   72 ( 2 )   141 - 143   2020.04

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    Kind of work：Joint Work  

  • Deep sequence analysis of NS5A resistance-associated substitution changes in patients reinfected with the hepatitis C virus after liver transplantation. Reviewed

    Yamamichi S, Miuma S, Wada T, Masumoto H, Kanda Y, Shibata H, Miyaaki H, Taura N, Ichikawa T, Yamamoto T, Nakao K

    Journal of viral hepatitis   2020.01（ ISSN:1352-0504 ）

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    Kind of work：Joint Work  

    DOI： 10.1111/jvh.13256

    PubMed

  • 水平ゲノム転移を介したvanD5バンコマイシン耐性遺伝子を保有するEnterococcus faeciumの出現(Emergence of vanD5-type vancomycin-resistant Enterococcus faecium via horizontal genomic transfer)

    佐藤 豊孝, 和田 崇之, 福島 由華里, 中島 千絵, 鈴木 定彦, 高橋 聡, 横田 伸一

    日本細菌学雑誌   75 ( 1 )   100 - 100   2020.01（ ISSN:0021-4930 ） （ eISSN:1882-4110 ）

  • Pseudo-outbreak of Mycobacterium chimaera through aerators of hand-washing machines at a hematopoietic stem cell transplantation center. Reviewed

    Nakamura S, Azuma M, Sato M, Fujiwara N, Nishino S, Wada T, Yoshida S

    Infection Control & Hospital Epidemiology   40 ( 12 )   1433 - 1435   2019.12

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    Kind of work：Joint Work  

    DOI： 10.1017/ice.2019.268

  • Distribution of Carnivore protoparvovirus 1 in free-living leopard cats (Prionailurus bengalensis chinensis) and its association with domestic carnivores in Taiwan Reviewed International coauthorship

    Chen CC, Chang AM, Wada T, Chen MT, Tu YS

    PLoS ONE   14 ( 9 )   e0221990   2019.09

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    Kind of work：Joint Work  

    DOI： 10.1371/journal.pone.0221990

  • Draft genome sequence of Vibrio harveyi strain GAN1807 isolated from diseased greater amberjack (Seriola dumerili) farmed in Japan in 2018.

    Kato Y, Wada T, Yamashita H, Ikeda T, Nishiyama K, Imajoh M

    Microbiology Resource Announcements   8 ( 34 )   e00629-19   2019.08

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  • Longitudinal genotyping surveillance of Mycobacterium tuberculosis in an area with high tuberculosis incidence shows high transmission rate of the modern Beijing subfamily in Japan. Reviewed

    Yamamoto K, Takeuchi S, Seto J, Shimouchi A, Komukai J, Hase A, Nakamura H, Umeda K, Hirai Y, Matsumoto K, Ogasawara J, Wada T, Yamamoto T

    Infection, genetics and evolution   72   25 - 30   2019.08（ ISSN:1567-1348 ）

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    Kind of work：Joint Work  

    DOI： 10.1016/j.meegid.2018.09.014

    PubMed

  • Is the network of heterosexual contact in Japan scale free?

    Ito H, Tamura K, Wada T, Yamamoto T, Morita S

    PLoS ONE   14 ( 8 )   e0221520   2019.08

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  • Draft genome sequences of three Flavobacterium psychrophilum strains isolated from diseased ayu (Plecoglossus altivelis altivelis) caught at three sites in Kagami River, Kochi, Japan.

    Yamashita H, Wada T, Kato Y, Ikeda T, Imajoh M

    Microbiology Resource Announcements   8 ( 34 )   e00773-19   2019.08

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  • Establishment of loop-mediated isothermal amplification for rapid and convenient detection of Mycobacterium marinum complex.

    Tsai MA, Wang PC, Yoshida S, Aono A, Mitarai S, Wada T, Chen SC

    Journal of Microbiological Methods   164   105671   2019.07

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  • Draft genome sequences of six Flavobacterium psychrophilum strains isolated from dead juvenile ayu (Plecoglossus altivelis altivelis) near the mouth of the Nahari River, Kochi, Japan.

    Yamashita H, Wada T, Kato Y, Ikeda T, Imajoh M

    Microbiology Resource Announcements   8 ( 29 )   e00759-19   2019.07

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  • Host range-associated clustering based on multilocus variable-number tandem-repeat analysis, phylotypes, and virulence genes of atypical enteropathogenic Escherichia coli strains.

    Parvej MS, Nakamura H, Alam MA, Wang L, Zhang S, Emura K, Kage-Nakadai E, Wada T, Hara-Kudo Y, Nishikawa Y

    Applied and environmental microbiology   85 ( 6 )   2019.03（ ISSN:0099-2240 ）

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  • Nosocomial Mycobacterium tuberculosis transmission by brief casual contact identified using comparative genomics.

    Seto J, Otani Y, Wada T, Suzuki Y, Ikeda T, Araki K, Mizuta K, Ahiko T

    The Journal of hospital infection   2019.01（ ISSN:0195-6701 ）

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  • Mycobacterium caprae Infection in Captive Borneo Elephant, Japan.

    Yoshida S, Suga S, Ishikawa S, Mukai Y, Tsuyuguchi K, Inoue Y, Yamamoto T, Wada T

    Emerging infectious diseases   24 ( 10 )   1937 - 1940   2018.10（ ISSN:1080-6040 ）

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  • A case of laboratory cross-contamination of Mycobacterium tuberculosis identified using comparative genomics.

    Seto J, Wada T, Suzuki Y, Ikeda T, Araki K, Umetsu Y, Ishikawa H, Mizuta K, Ahiko T

    The international journal of tuberculosis and lung disease   22 ( 10 )   1239 - 1242   2018.10（ ISSN:1027-3719 ）

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  • Current health status and its risk factors of the Tsarang villagers living at high altitude in the Mustang district of Nepal.

    Koirala S, Nakano M, Arima H, Takeuchi S, Ichikawa T, Nishimura T, Ito H, Pandey BD, Pandey K, Wada T, Yamamoto T

    Journal of physiological anthropology   37 ( 1 )   20   2018.08（ ISSN:1880-6791 ）

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  • Phylogenetic uniqueness of Mycobacterium avium subspecies hominissuis isolated from an abnormal pulmonary bovine case.

    Yoshida S, Araki T, Asai T, Tsuyuguchi K, Arikawa K, Iwamoto T, Nakajima C, Suzuki Y, Ohya K, Yanai T, Wada T, Yamamoto T

    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases   62   122 - 129   2018.08（ ISSN:1567-1348 ）

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  • ネパールのムスタン郡高地に居住するツァラン村民の健康状態の現状と危険因子(Current health status and its risk factors of the Tsarang villagers living at high altitude in the Mustang district of Nepal)

    Koirala Sweta, Nakano Masayuki, Arima Hiroaki, Takeuchi Shouhei, Ichikawa Tomo, Nishimura Takayuki, Ito Hiromu, Dev Pandey Basu, Pandey Kishor, Wada Takayuki, Yamamoto Taro

    日本生理人類学会　Journal of Physiological Anthropology   37 ( August )   1 - 10   2018.08（ ISSN:1880-6791 ）

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    ネパールのムスタン群において、3570m高地に居住するツァラン村の住民を対象とし、非感染性疾患(NCD)の有病率と危険因子について横断調査を実施した。18歳以上の住民から無作為に選択した被験者188例に対しアンケート調査を行い、糖化ヘモグロビン(HbA1c)測定を含む生化学的検査および理学的検査を施行した。その結果、中程度の高血糖症および糖尿病の有病率はそれぞれ31.6%および4.6%で、血中酸素飽和度(SpO2)が90%未満である低酸素血症の有病率は27.1%であった。また、多重ロジスティック回帰分析からは、HbA1c≧6%の耐糖能異常有病率に関連する因子として、高年齢とSpO2低下であることが明らかにされた。以上より、ネパール高地人ではグルコース代謝障害で有病率が高く、要因として、高地生活の低酸素条件下による低酸素血症に関与するものと考えられた。

  • Whole genome analysis of a multidrug-resistant Streptococcus pneumoniae isolate from a patient with invasive pneumococcal infection developing disseminated intravascular coagulation.

    Ohkoshi Y, Sato T, Wada T, Fukushima Y, Murabayashi H, Takakuwa Y, Nishiyama K, Honda H, Shiraishi T, Kuronuma K, Takahashi H, Nakajima C, Suzuki Y, Yokota SI

    Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy   24 ( 8 )   674 - 681   2018.08（ ISSN:1341-321X ）

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  • Disseminated nontuberculous mycobacterial disease in a cat caused by Mycobacterium sp. strain MFM001.

    Kayanuma H, Ogihara K, Yoshida S, Yamamoto K, Wada T, Yamamoto T, Tsuyuki Y, Madarame H

    Veterinary microbiology   220   90 - 96   2018.07（ ISSN:0378-1135 ）

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  • Complete genome sequence of multidrug-resistant Streptococcus pneumoniae serotype 19F isolated from an invasive infection in Sapporo, Japan.

    Sato T, Ohkoshi Y, Wada T, Fukushima Y, Murabayashi H, Takakuwa Y, Nishiyama K, Shiraishi T, Nakajima C, Suzuki Y, Yokota SI

    Genome announcements   5 ( 44 )   2017.11

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  • Convenient PCR method for variable-number tandem-repeat typing of Mycobacterium tuberculosis clinical isolates

    Junji Seto, Takayuki Wada, Yu Suzuki, Tatsuya Ikeda, Katsumi Mizuta, Satoshi Mitarai, Tadayuki Ahiko

    ELSEVIER SCIENCE BV　JOURNAL OF MICROBIOLOGICAL METHODS   139   12 - 14   2017.08（ ISSN:0167-7012 ）

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    Variable-number tandem-repeat typing for Mycobacterium tuberculosis clinical isolates contributes to evidence based tuberculosis control. However, cumbersome PCR procedures for the typing have disturbed routine analyses. We proposed a convenient PCR method for the typing using a PCR master mix that provides rapidity and long-term stability of the frozen PCR cocktail.

    DOI： 10.1016/j.mimet.2017.04.008

    PubMed

  • Complete genome sequences of three representative Mycobacterium tuberculosis Beijing family strains belonging to distinct genotype clusters in Hanoi, Vietnam, during 2007 to 2009.

    Wada T, Hijikata M, Maeda S, Hang NTL, Thuong PH, Hoang NP, Hung NV, Keicho N

    Genome announcements   5 ( 27 )   2017.07

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  • Complete genome sequence of a Mycobacterium tuberculosis strain belonging to the East African-Indian family in the Indo-Oceanic lineage, isolated in Hanoi, Vietnam.

    Wada T, Hijikata M, Maeda S, Hang NTL, Thuong PH, Hoang NP, Hung NV, Keicho N

    Genome announcements   5 ( 24 )   2017.06

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  • Draft genome sequence of clinical strain TANI1 of Streptococcus suis serotype 5 isolated from a bacteremia patient in Japan.

    Yoshida H, Wada T, Taniyama D, Takahashi T

    Genome announcements   5 ( 18 )   2017.05

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  • Mycobacterium tuberculosis Transmission among Elderly Persons, Yamagata Prefecture, Japan, 2009-2015

    Junji Seto, Takayuki Wada, Yu Suzuki, Tatsuya Ikeda, Katsumi Mizuta, Taro Yamamoto, Tadayuki Ahiko

    CENTERS DISEASE CONTROL　EMERGING INFECTIOUS DISEASES   23 ( 3 )   448 - 455   2017.03（ ISSN:1080-6040 ）

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    In many countries with low to moderate tuberculosis (TB) incidence, cases have shifted to elderly persons. It is unclear, however, whether these cases are associated with recent Mycobacterium tuberculosis transmission or represent reactivation of past disease. During 2009-2015, we performed a population-based TB investigation in Yamagata Prefecture, Japan, using in-depth contact tracing and 24-loci variable-number tandem-repeat typing optimized for Beijing family M. tuberculosis strains. We analyzed 494 strains, of which 387 (78.3%) were derived from elderly patients. Recent transmission with an epidemiologic link was confirmed in 22 clusters (70 cases). In 17 (77.3%) clusters, the source patient was elderly; 11(64.7%) of the 17 clusters occurred in a hospital or nursing home. In this setting, the increase in TB cases was associated with M. tuberculosis transmissions from elderly persons. Prevention of transmission in places where elderly persons gather will be an effective strategy for decreasing TB incidence among predominantly elderly populations.

    DOI： 10.3201/eid2303.161571

    PubMed

  • Mycolactone-producing Mycobacterium marinum infection in captive Hong Kong warty newts and pathological evidence of impaired host immune function

    Wen-Ta Li, Hui-Wen Chang, Victor Fei Pang, Fun-In Wang, Chen-Hsuan Liu, Ting-Yu Chen, Jun-Cheng Guo, Takayuki Wada, Chian-Ren Jeng

    INTER-RESEARCH　DISEASES OF AQUATIC ORGANISMS   123 ( 3 )   239 - 249   2017.03（ ISSN:0177-5103 ）

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    A mass mortality event of captive Hong Kong warty newts Paramesotriton hong kongensis with non-granulomatous necrotic lesions occurred in Taipei Zoo, Taiwan, in 2014. Clinically, the sick newts were lethargic and often covered with water mold Saprolegnia sp. on the skin of the body trunk or extremities. Predominant pathological findings were multifocal non-granulomatous necrotic lesions in the liver, spleen, and kidneys and severe skin infection with Sapro legnia sp., with deep invasion and involvement of underlying muscles. The possibility of ranavirus infection was ruled out by negative PCR results. Unexpectedly, abundant intralesional acid-fast positive bacilli were found in the necrotic lesions of the liver, spleen, and kidney in all 14 sick newts. PCR targeting the hsp65, ITS region, and partial 16S rRNA genes was performed, and the sequence identity from amplified amplicons of hsp65 and partial 16S rRNA genes was 100% identical to that of the corresponding gene fragment of Mycobacterium marinum. Further molecular investigations demonstrated that the current M. marinum was a mycolactone-producing myco bacterium with the presence of esxA/esxB genes. Mycolactone is a plasmid-encoded, immunosuppressive, and cytotoxic toxin. The possible immunosuppression phenomenon characterized by systemic non-granulomatous necrotic lesions caused by M. marinum and the unusual deep invasive infection caused by water mold might be associated with the immunosuppressive effect of mycolactone. Therefore, it should be noted that non-granulomatous necrotic lesions in amphibians can be caused not only by ranavirus infection but also by mycobacteriosis.

    DOI： 10.3354/dao03092

    PubMed

  • 東海地方の家畜及び農場内外の環境から分離された非結核性抗酸菌の遺伝子特性

    澤井 宏太郎, 菅沼 彰太, 松尾 加代子, 大津 桂子, 野崎 恵子, 和田 崇之, 丸山 史人, 西内 由紀子, 福士 秀人, 岩本 朋忠, 大屋 賢司

    日本細菌学雑誌   72 ( 1 )   165 - 165   2017.02（ ISSN:0021-4930 ） （ eISSN:1882-4110 ）

  • 家畜及び農場内外の環境から分離された非結核性抗酸菌の遺伝子特性

    菅沼 彰太, 澤井 宏太郎, 松尾 加代子, 大津 桂子, 和田 崇之, 丸山 史人, 西内 由紀子, 岩本 朋忠, 福士 秀人, 大屋 賢司

    日本獣医学会学術集会講演要旨集   159回   356 - 356   2016.08（ ISSN:1347-8621 ）

  • Tumor in chest wall caused by Mycobacterium bovis BCG infection: Identification on polymerase chain reaction of formalin-fixed specimen

    Koji Yokoyama, Kenji Kubo, Shiomi Yoshida, Takayuki Wada, Tomoki Kawai, Ryuta Nishikomori, Akira Yoshida

    WILEY-BLACKWELL　PEDIATRICS INTERNATIONAL   58 ( 4 )   317 - 318   2016.04（ ISSN:1328-8067 ）

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  • 結核菌全ゲノム解析を活用した「M株-神戸市分離株」の感染・伝搬様式の推定

    岩本 朋忠, 有川 健太郎, 村瀬 良朗, 横山 真一, 南谷 千絵, 和田 崇之, 藤山 理世

    結核   91 ( 3 )   309 - 309   2016.03（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

  • Characterization of unstable pEntYN10 from enterotoxigenic Escherichia coli (ETEC) O169:H41.

    Ban E, Yoshida Y, Wakushima M, Wajima T, Hamabata T, Ichikawa N, Abe H, Horiguchi Y, Hara-Kudo Y, Kage-Nakadai E, Yamamoto T, Wada T, Nishikawa Y

    Virulence   6 ( 8 )   735 - 744   2015.12（ ISSN:2150-5594 ）

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  • Deep sequencing analysis of the heterogeneity of seed and commercial lots of the bacillus Calmette-Guérin (BCG) tuberculosis vaccine substrain Tokyo-172.

    Wada T, Maruyama F, Iwamoto T, Maeda S, Yamamoto T, Nakagawa I, Yamamoto S, Ohara N

    Scientific reports   5   17827   2015.12

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  • JATA(12)-VNTR結核菌型別法による2014年京都府分離菌株の解析

    浅井 紀夫, 平田 佐知, 真田 正稔, 鷲見 舞, 堀 忍, 中村 清康, 和田 崇之, 長谷 篤

    京都府保健環境研究所　京都府保健環境研究所年報   ( 60 )   12 - 15   2015.12（ ISSN:1341-4232 ）

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    Japan Anti-Tuberculosis Association(12)-VNTR[JATA(12)-VNTR]結核菌型別解析法のプロトコルに基づいて、2014年4月から12月に新規登録された結核患者から分離された24菌株の型別を調査し、既に報告した103菌株と合わせた127菌株についてクラスター分類を行った。今回解析した24菌株のうち5菌株が関連するクラスターは1つ、2菌株が関連するクラスターは3つ、1菌株が関連するクラスターは5つの合計9クラスターに分類され、16菌株がいずれかのクラスターに包含された。また、実地疫学調査では個別の感染経路については解明できなかったが、多くの菌株で構成されるクラスターは和田らが報告した阪神地区において出現頻度の高いJATA(12)-VNTR型別に一致し、京都府内でも同様の型別の流行があると予測される。

  • 大阪市における結核菌の分子疫学解析状況(2012-2014)

    山本 香織, 中村 寛海, 小向 潤, 和田 崇之, 梅田 薫, 小笠原 準, 平井 有紀, 平山 照雄, 長谷 篤, 松本 健二, 西尾 孝之

    (地独)大阪健康安全基盤研究所　大阪市立環境科学研究所報告 調査・研究年報   ( 77 )   21 - 26   2015.12（ ISSN:0285-5801 ）

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    2012年1月〜2014年12月の間の大阪市における新登録結核患者は3204名であった。今回、結核菌が分離搬入された1654株中、687株のJATA(12)-VNTR型別を実施した。その結果、1)687株のうち、ハイリスクグループはホームレス患者由来68株、外国出生患者28株、若年患者由来187株、多剤耐性結核菌(MDR)患者由来18株であった。2)JATA(12)-VNTR型別より12領域での反復数が一致したものをクラスターと定義したところ、419株(61.0%)が86クラスターを形成しており、クラスタータイプ別の出現割合はJ12-0001が40株(5.8%)と最も多く、2006〜2011年の出現割合とほぼ同様であった。3)M株を示すクラスタータイプのJ12-0006は11株検出され、ホームレス患者由来の68株の最も大きなクラスターは7株から形成されるJ12-0010 で、このうち6株は追加領域を含めた24領域のVNTR型が全て一致した。4)外国出生患者由来28株の14株が国内出生患者由来株と11クラスターを形成していたが、このうち5クラスターは追加領域のVNTR型が不一致であり、国内出生患者由来株とは異なる菌株であることが判明した。5)若年患者由来187株間では91株が30クラスターを形成し、最も大きなクラスターはJ12-0001とクラスターG各々9株であった。尚、MDR18株中3株はJ12-0022で、このタイプの菌株が出現した場合はMDRの可能性に注意が必要と考えられた。

  • Primary Gingival Tuberculosis Diagnosed Based on Genetic Identification.

    Okano Y, Yoshida S, Shinohara T, Takahashi N, Naito N, Kagawa K, Machida H, Hatakeyama N, Ohno A, Wada T, Ogushi F

    Internal medicine (Tokyo, Japan)   54 ( 21 )   2765 - 2768   2015.11（ ISSN:0918-2918 ）

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  • Phylogenetic assignment of Mycobacterium tuberculosis Beijing clinical isolates in Japan by maximum a posteriori estimation.

    Seto J, Wada T, Iwamoto T, Tamaru A, Maeda S, Yamamoto K, Hase A, Murakami K, Maeda E, Oishi A, Migita Y, Yamamoto T, Ahiko T

    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases   35   82 - 88   2015.10（ ISSN:1567-1348 ）

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  • Comparative analysis of Mycobacterium tuberculosis Beijing strains isolated in three remote areas of Japan.

    Yokoyama E, Hachisu Y, Iwamoto T, Nakanishi N, Arikawa K, Wada T, Seto J, Kishida K

    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases   34   444 - 449   2015.08（ ISSN:1567-1348 ）

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  • Maternal overweight/obesity characteristics and child anthropometric status in Jos, Nigeria.

    John C, Ichikawa T, Abdu H, Ocheke I, Diala U, Modise-Letsatsi V, Wada T, Okolo S, Yamamoto T

    Nigerian medical journal : journal of the Nigeria Medical Association   56 ( 4 )   236 - 239   2015.07（ ISSN:0300-1652 ）

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  • Characterization of Mycolic Acids in Total Fatty Acid Methyl Ester Fractions from Mycobacterium Species by High Resolution MALDI-TOFMS.

    Teramoto K, Suga M, Sato T, Wada T, Yamamoto A, Fujiwara N

    Mass spectrometry (Tokyo, Japan)   4 ( 1 )   A0035   2015.03（ ISSN:2187-137X ）

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  • Clonality and micro-diversity of a nationwide spreading genotype of Mycobacterium tuberculosis in Japan.

    Wada T, Iwamoto T, Tamaru A, Seto J, Ahiko T, Yamamoto K, Hase A, Maeda S, Yamamoto T

    PLoS ONE   10 ( 3 )   e0118495   2015.03

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  • JATA(12)-VNTR結核菌型別解析法の検討と京都府分離菌株の解析結果

    浅井 紀夫, 平田 佐知, 真田 正稔, 鷲見 舞, 堀 忍, 中村 清康, 和田 崇之, 長谷 篤

    京都府保健環境研究所　京都府保健環境研究所年報   ( 59 )   8 - 12   2014.12（ ISSN:1341-4232 ）

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    Japan Anti-Tuberculosis Association(12)-VNTR結核菌型別解析法において高分子量領域に対応できるアガロースゲル電気泳動法について検討し、バイオラッド製Sub-Cell GT電気泳動槽を用いて360分間の泳動を行うことで解析できることを確認した。本法を用いて2013年度に分離・保存された菌株43株の解析を行った結果、既報60菌株と併せ、10菌株、5菌株、4菌株、3菌株、2菌株で構成されるクラスターがそれぞれ1個、1個、3個、3個、8個と、クラスターを構成しない菌株に分類された。今回解析の43株に関連する10個のクラスター型について実地疫学調査を行ったところ、山城北保健所管内と丹後保健所管内の両方の菌株が含まれた5個のクラスターでは患者間の関連性を解明できなかったが、一方のみの菌株で構成された5個中2個のクラスターの事例では感染経路が解明され、結核対策に結びつけることができた。

  • Mycobacterium marinum Infection in Japanese Forest Green Tree Frogs (Rhacophorus arboreus)

    M. Haridy, Y. Tachikawa, S. Yoshida, K. Tsuyuguchi, M. Tomita, S. Maeda, T. Wada, K. Ibi, H. Sakai, T. Yanai

    ELSEVIER SCI LTD　JOURNAL OF COMPARATIVE PATHOLOGY   151 ( 2-3 )   277 - 289   2014.08（ ISSN:0021-9975 ）

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    Four Japanese forest green tree frogs (Rhacophorus arboreus) were presented with emaciation, abdominal distention and ulcerative and nodular cutaneous lesions affecting the brisket, limbs, digits and ventral abdomen. Another three frogs had been found dead in the same tank 1 year previously. Necropsy examination of these seven frogs revealed splenomegaly and hepatomegaly, with multiple tan-yellow nodular foci present in the liver, spleen, heart, lungs, ovaries and kidneys. Microscopically, five frogs had necrosis and surrounding granulomatous inflammation in the liver, spleen, kidneys, lungs, intestine and ovaries, with numerous acid-fast bacilli in the areas of necrosis. Two frogs had granulomatous lesions in the lungs, liver, spleen, heart, coelomic membrane, stomach and intestinal wall. These lesions had no or minimal necrosis and few acid-fast bacilli. Mycobacterium spp. was cultured from three frogs and identified as Mycobacterium marinum by colony growth rate and photochromogenicity and DNA sequencing. This is the first report of M. marinum infection in Japanese forest green tree frogs. (C) 2014 Elsevier Ltd. All rights reserved.

    DOI： 10.1016/j.jcpa.2014.04.014

    PubMed

  • Distribution of Capnocytophaga canimorsus in dogs and cats with genetic characterization of isolates

    Kaoru Umeda, Risa Hatakeyama, Takuto Abe, Koh-Ichi Takakura, Takayuki Wada, Jun Ogasawara, Shu-Ichi Sanada, Atsushi Hase

    ELSEVIER SCIENCE BV　VETERINARY MICROBIOLOGY   171 ( 1-2 )   153 - 159   2014.06（ ISSN:0378-1135 ）

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    Capnocytophaga canimorsus, which is often found in the oral cavities of dogs and cats, is sometimes transmitted to humans, causing severe infection. To elucidate the risk of C canimorsus in humans and animals, this study was undertaken to characterize this bacterium epidemiologically and genetically. We examined the distribution of C canimorsus in dogs and cats, and analyzed the correlation between the presence of bacteria and individual factors statistiCally. We also compared C canimorsus isolates genetically using 16S rRNA gene sequence analysis and pulsed-field gel electrophoresis (PFGE). C canimorsus was detected in 76 of 109 dogs (69.7%) and 57 of 104 cats (54.8%). A relation between C canimorsus presence and some individual factors was detected both in dogs and cats, but the predictive factors of carrying the bacterium differed between dogs and cats. 16S rRNA gene sequences from C canimorsus isolates in this study were combined with previously published sequences to assess their intra-specific phylogeny. Results show that C. canimorsus is classifiable into two main groups (I and II) with differing -y-glutamyl aminopeptidase activity. Strains from human patients belonged unevenly to group I, possibility suggesting that group I can be transmitted to humans and group II is indigenous only to the oral cavities of dogs and cats. PFGE genotyping showed high discriminatory power, and the dendrogram accorded with genetic segregation between isolates of group land II. Sma I-digest PFGE developed for this study is useful as a molecular typing method for additional epidemiological and phylogenetic studies of C canimorsus. (C) 2014 Elsevier B.V. All rights reserved.

    DOI： 10.1016/j.vetmic.2014.03.023

    PubMed

  • Second worldwide proficiency study on variable number of tandem repeats typing of Mycobacterium tuberculosis complex

    J. L. de Beer, C. Kodmon, J. van Ingen, P. Supply, D. van Soolingen

    INT UNION AGAINST TUBERCULOSIS LUNG DISEASE (I U A T L D)　INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE   18 ( 5 )   594 - 600   2014.05（ ISSN:1027-3719 ）

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    BACKGROUND: The quality of variable number of tandem repeats (VNTR) typing of Mycobacterium tuberculosis was first investigated in 2009 in 37 laboratories worldwide. The results revealed an inter- and intra-laboratory reproducibility of respectively 60% and 72%. These data spurred an improvement in laboratory-specific assays and global standardisation of VNTR typing.
    OBJECTIVE: To measure the effects of the technical improvements and increased standardisation, a test panel consisting of 30 M. tuberculosis complex DNA samples was distributed for VNTR typing in 41 participating laboratories from 36 countries.
    RESULTS: The inter- and intra-laboratory reproducibility increased overall to respectively 78% and 88%. The 33 laboratories that participated in both the first and second proficiency studies improved their inter- and intra-laboratory reproducibility from 62% and 72% to respectively 79% and 88%. The largest improvement in reproducibility was detected in 10 laboratories that use an in-house polymerase chain reaction technique and perform amplicon sizing using gel electrophoresis. Detailed error analysis revealed a reduction in the number of systematic errors, sample exchange events and non-amplifiable loci.
    CONCLUSION: This second worldwide proficiency study indicates a substantial increase in the reproducibility of VNTR typing of M. tuberculosis. This will contribute to a more meaningful interpretation of molecular epidemiological and phylogenetic studies on the M. tuberculosis complex.

    DOI： 10.5588/ijtld.13.0531

    PubMed

  • 結核菌における遺伝型別一致株の比較ゲノム解析

    和田 崇之, 岩本 朋忠, 前田 伸司, 長谷 篤, 山本 太郎, 瀬戸 順次, 田丸 亜貴

    日本細菌学雑誌   69 ( 1 )   211 - 211   2014.02（ ISSN:0021-4930 ） （ eISSN:1882-4110 ）

  • 2012年に大阪市内の食中毒原因調査において検出された下痢原性微生物と主な事件の概要

    長谷 篤, 小笠原 準, 中村 寛海, 和田 崇之, 梅田 薫, 山本 香織, 入谷 展弘, 阿部 仁一郎, 久保 英幸, 改田 厚, 山元 誠司, 平山 照雄, 平井 有紀, 後藤 薫

    (地独)大阪健康安全基盤研究所　大阪市立環境科学研究所報告 調査・研究年報   ( 75 )   23 - 29   2013.12（ ISSN:0285-5801 ）

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    大阪市内で食中毒が疑われ、当研究所に検体が搬入された事件についてまとめ、その原因病原体と疫学情報を基に感染源を明らかにした。食中毒が疑われた患者の便と吐物、食品残品、原因と考えられる施設(患者宅を含む)に保存されていた検食と同施設のふきとり水および調理従事者の便と手指のふきとり水を調査の対象とした。その結果、2012年に食中毒、有症苦情などで検体が搬入されたのは117事件の検体で、68件に下痢原性微生物が検出された。なお68件の内訳はノロウイルス47件(69.1%)、カンピロバクター7件(10.3%)、サルモネラ属菌5件(7.4%)、黄色ブドウ球菌、クドア・セプテンプンクタータ2件(2.9%)、毒素原性大腸菌0153、腸管出血性大腸菌026、セレウス菌、サポウイルスが各1件、そして黄色ブドウ球菌とカンピロバクターが同時に検出された事件も1件に認められた。68件のうち、推定原因食品が判明した事件数は15件(22.1%)であり、15件のうち検出された原因微生物の内訳はノロウイルスが10件、クドア2件、サルモネラ1件、黄色ブドウ球菌1件、セレウス菌1件であり、原因施設が特定された事件数は63件(92.6%)に認められ、推定された原因食品は生カキ、弁当、寿司、コース料理、スパイスソース、ヒラメ、カレー、刺身などで、原因施設は飲食店が例年通り最も多く認められた。大阪市における細菌あるいはウイルス性食中毒事件として厚生労働省に届けられた事件数は24件であったが、全国的にもノロウイルス、カンピロバクターの食中毒事件の占める割合が多く、今後も十分な注意が必要であり、消費者にはきちんと加熱して食する習慣を守ること、平常時を含めた二次感染防止策の徹底・予防の啓発が重要と考えられた。

  • 京都府分離結核菌株のJATA(12)-VNTR型別解析　結核対策におけるJATA(12)-VNTR型別法の有用性

    浅井 紀夫, 杉浦 伸明, 真田 正稔, 堀 忍, 中村 清康, 和田 崇之, 長谷 篤

    京都府保健環境研究所　京都府保健環境研究所年報   ( 58 )   6 - 11   2013.11（ ISSN:1341-4232 ）

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    前田らが確立した結核菌分類法のJapan Anti-Tuberculosis Association(JATA) 12-VNTR(Variable Number of Tandem Repeats)を用い、2004年4月〜2012年3月に分離・保存した42菌株と2012年4月〜2013年3月に入手した18菌株の合計60菌株の型別解析を行った。その結果、60菌株のVNTR型は45のパターンに分類され、このうち24菌株で9個のクラスターを形成した。このクラスターごとに感染経路調査を実施したところ、9事例中3事例で解明され、うち1例は追跡調査に発展した。JAYA(12)-VNTR型別解析は、菌株間の関連が明白な場合や感染源・経路が実地疫学的に予想されている場合には極めて有用であり、これらの結果は結核対策の科学的裏付けや追跡調査に活用できると考えられた。

  • Whole-Genome Sequence of the Potentially Hypertransmissible Multidrug-Resistant Mycobacterium tuberculosis Beijing Strain OM-V02_005.

    Tateishi Y, Tamaru A, Ogura Y, Niki M, Wada T, Yamamoto T, Hirata K, Hayashi T, Matsumoto S

    Genome announcements   1 ( 4 )   2013.08

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  • Evolutionary robust SNPs reveal the misclassification of Mycobacterium tuberculosis Beijing family strains into sublineages.

    Nakanishi N, Wada T, Arikawa K, Millet J, Rastogi N, Iwamoto T

    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases   16   174 - 177   2013.06（ ISSN:1567-1348 ）

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  • Multi-locus variable number tandem repeat analysis for Clostridium botulinum type B isolates in Japan: comparison with other isolates and genotyping methods.

    Umeda K, Wada T, Kohda T, Kozaki S

    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases   16   298 - 304   2013.06（ ISSN:1567-1348 ）

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  • Effectiveness of comprehensive variable number of tandem repeat (VNTR) analysis in areas with a low incidence of tuberculosis.

    Seto J, Ahiko T, Wada T, Hase A, Yamada K

    Kekkaku   88 ( 6 )   535 - 542   2013.06（ ISSN:0022-9776 ）

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  • Evaluation of JATA(12)-variable number of tandem repeats as a marker of the source of tuberculosis outbreaks in Osaka.

    Tamaru A, Wada T, Iwamoto T, Hase A

    Kekkaku   88 ( 4 )   399 - 403   2013.04（ ISSN:0022-9776 ）

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  • Multiplex agarose gel electrophoresis system for variable number of tandem repeats genotyping: analysis example using Mycobacterium tuberculosis.

    Wada T, Maeda S

    Electrophoresis   34 ( 8 )   1171 - 1174   2013.04（ ISSN:0173-0835 ）

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  • Clustering analysis of Mycobacterium tuberculosis using the JATA(12)-VNTR system for molecular epidemiological surveillance in broad areas of Japan.

    Wada T, Tamaru A, Iwamoto T, Arikawa K, Nakanishi N, Komukai J, Matsumoto K, Hase A

    Kekkaku   88 ( 4 )   393 - 398   2013.04（ ISSN:0022-9776 ）

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  • 2011年に大阪市内の食中毒原因調査において検出された下痢原性微生物

    長谷 篤, 小笠原 準, 中村 寛海, 和田 崇之, 梅田 薫, 入谷 展弘, 久保 英幸, 改田 厚, 関口 純一朗, 阿部 仁一郎, 濱田 信夫, 大山 み乃り, 平山 照雄, 後藤 薫

    (地独)大阪健康安全基盤研究所　大阪市立環境科学研究所報告 調査・研究年報   ( 74 )   11 - 16   2012.12（ ISSN:0285-5801 ）

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    2011年に大阪市内の食中毒原因調査において検出された下痢原性微生物について報告した。対象は119事例1123検体で、68件より下痢原性微生物が検出された。内訳はノロウイルス38件、カンピロバクター19件、サルモネラ属菌3件、黄色ブドウ球菌2件、ウェルシュ菌、毒素原性大腸菌O159、腸管出血性大腸菌O157、セレウス菌、サポウイルス各1件で、クドア・セプテンプンクタータ/カンピロバクター同時検出が1件であった。ノロウイルスの検出は12〜1月がピークで、2010年と同様にGenogroup IIが主流であったが、Genogroup Iも5件検出された。原因食品でカキ関連が明らかになっていたのは2件のみで、他の食品関連が例年同様多かった。カンピロバクターは2009年から連続して増加しており、原因食品は不明が多かった。全68件のうち推定原因食品が判明したのは37件、原因施設が特定されたのは45件であった。

  • Genetic Diversity and Transmission Characteristics of Beijing Family Strains of Mycobacterium tuberculosis in Peru

    Tomotada Iwamoto, Louis Grandjean, Kentaro Arikawa, Noriko Nakanishi, Luz Caviedes, Jorge Coronel, Patricia Sheen, Takayuki Wada, Carmen A. Taype, Marie-Anne Shaw, David A. J. Moore, Robert H. Gilman

    PUBLIC LIBRARY SCIENCE　PLOS ONE   7 ( 11 )   e49651   2012.11（ ISSN:1932-6203 ）

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    Beijing family strains of Mycobacterium tuberculosis have attracted worldwide attention because of their wide geographical distribution and global emergence. Peru, which has a historical relationship with East Asia, is considered to be a hotspot for Beijing family strains in South America. We aimed to unveil the genetic diversity and transmission characteristics of the Beijing strains in Peru. A total of 200 Beijing family strains were identified from 2140 M. tuberculosis isolates obtained in Lima, Peru, between December 2008 and January 2010. Of them, 198 strains were classified into sublineages, on the basis of 10 sets of single nucleotide polymorphisms (SNPs). They were also subjected to variable number tandem-repeat (VNTR) typing using an international standard set of 15 loci (15-MIRU-VNTR) plus 9 additional loci optimized for Beijing strains. An additional 70 Beijing family strains, isolated between 1999 and 2006 in Lima, were also analyzed in order to make a longitudinal comparison. The Beijing family was the third largest spoligotyping clade in Peru. Its population structure, by SNP typing, was characterized by a high frequency of Sequence Type 10 (ST10), which belongs to a modern subfamily of Beijing strains (178/198, 89.9%). Twelve strains belonged to the ancient subfamily (ST3 [n = 3], ST25 [n = 1], ST19 [n = 8]). Overall, the polymorphic information content for each of the 24 loci values was low. The 24 loci VNTR showed a high clustering rate (80.3%) and a high recent transmission index (RTIn-1 = 0.707). These strongly suggest the active and ongoing transmission of Beijing family strains in the survey area. Notably, 1 VNTR genotype was found to account for 43.9% of the strains. Comparisons with data from East Asia suggested the genotype emerged as a uniquely endemic clone in Peru. A longitudinal comparison revealed the genotype was present in Lima by 1999.

    DOI： 10.1371/journal.pone.0049651

    PubMed

  • Scanning of genetic diversity of evolutionarily sequential Mycobacterium tuberculosis Beijing family strains based on genome wide analysis

    Takayuki Wada, Tomotada Iwamoto, Atsushi Hase, Shinji Maeda

    ELSEVIER SCIENCE BV　INFECTION GENETICS AND EVOLUTION   12 ( 7 )   1392 - 1396   2012.10（ ISSN:1567-1348 ）

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    The Beijing family is an endemic lineage of Mycobacterium tuberculosis in eastern Asia. In Japan, five evolutionarily sequential sublineages composing the lineage have predominated. Comparative genomic sequencing based on a microarray technique was conducted for five representative strains of those respective sublineages. Results revealed approximately 200 point mutations specific to each strain. Subsequently, to investigate the genetic diversity of each sublineage, we analysed the phylogenetic divergence of 103 domestic strains belonging to them using genetic markers derived from the mutation information. Results show that the five sublineages have comprised smaller lineages which had diverged at various points. The smaller sub-sublineages have emerged with respective bottlenecks, which are reflected in the excessive monophyletic evolution of the species. Our data provide necessary information to grasp a comprehensive picture of genetic diversity of the lineage constructed in its evolution. (c) 2012 Elsevier B.V. All rights reserved.

    DOI： 10.1016/j.meegid.2012.04.029

    PubMed

  • Dominant Incidence of Multidrug and Extensively Drug-Resistant Specific Mycobacterium tuberculosis Clones in Osaka Prefecture, Japan

    Aki Tamaru, Chie Nakajima, Takayuki Wada, Yajun Wang, Manabu Inoue, Ryuji Kawahara, Ryoji Maekura, Yuriko Ozeki, Hisashi Ogura, Kazuo Kobayashi, Yasuhiko Suzuki, Sohkichi Matsumoto

    PUBLIC LIBRARY SCIENCE　PLOS ONE   7 ( 8 )   e42505   2012.08（ ISSN:1932-6203 ）

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    Infection and transmission of multidrug-resistant Mycobacterium tuberculosis (MDR-Mtb) and extensively drug-resistant M. tuberculosis (XDR-Mtb) is a serious health problem. We analyzed a total of 1,110 Mtb isolates in Osaka Prefecture and neighboring areas from April 2000 to March 2009. A total of 89 MDR-Mtb were identified, 36 (48.5%) of which were determined to be XDR-Mtb. Among the 89 MDR-Mtb isolates, 24 (27.0%) phylogenetically distributed into six clusters based on mycobacterial interspersed repetitive units-various number of tandem repeats (MIRU-VNTR) typing. Among these six clusters, the MIRU-VNTR patterns of four (OM-V02, OM-V03, OM-V04, and OM-V06) were only found for MDR-Mtb. Further analysis revealed that all isolates belonging to OM-V02 and OM-V03, and two isolates from OM-V04 were clonal. Importantly such genotypes were not observed for drug-sensitive isolates. These suggest that few but transmissible clones can transmit after acquiring multidrug resistance and colonize even in a country with a developed, well-organized healthcare system.

    DOI： 10.1371/journal.pone.0042505

    PubMed

  • First Worldwide Proficiency Study on Variable-Number Tandem-Repeat Typing of Mycobacterium tuberculosis Complex Strains

    Jessica L. de Beer, Kristin Kremer, Csaba Kodmon, Philip Supply, Dick van Soolingen

    AMER SOC MICROBIOLOGY　JOURNAL OF CLINICAL MICROBIOLOGY   50 ( 3 )   662 - 669   2012.03（ ISSN:0095-1137 ）

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    Although variable-number tandem-repeat (VNTR) typing has gained recognition as the new standard for the DNA fingerprinting of Mycobacterium tuberculosis complex (MTBC) isolates, external quality control programs have not yet been developed. Therefore, we organized the first multicenter proficiency study on 24-locus VNTR typing. Sets of 30 DNAs of MTBC strains, including 10 duplicate DNA samples, were distributed among 37 participating laboratories in 30 different countries worldwide. Twenty-four laboratories used an in-house-adapted method with fragment sizing by gel electrophoresis or an automated DNA analyzer, nine laboratories used a commercially available kit, and four laboratories used other methods. The intra-and interlaboratory reproducibilities of VNTR typing varied from 0% to 100%, with averages of 72% and 60%, respectively. Twenty of the 37 laboratories failed to amplify particular VNTR loci; if these missing results were ignored, the number of laboratories with 100% interlaboratory reproducibility increased from 1 to 5. The average interlaboratory reproducibility of VNTR typing using a commercial kit was better (88%) than that of in-house-adapted methods using a DNA analyzer (70%) or gel electrophoresis (50%). Eleven laboratories using in-house-adapted manual typing or automated typing scored inter-and intralaboratory reproducibilities of 80% or higher, which suggests that these approaches can be used in a reliable way. In conclusion, this first multicenter study has documented the worldwide quality of VNTR typing of MTBC strains and highlights the importance of international quality control to improve genotyping in the future.

    DOI： 10.1128/JCM.00607-11

    PubMed

  • 2010年に大阪市内の食中毒原因調査において検出された下痢原性微生物

    長谷 篤, 小笠原 準, 中村 寛海, 和田 崇之, 梅田 薫, 入谷 展弘, 久保 英幸, 改田 厚, 関口 純一朗, 阿部 仁一郎, 濱田 信夫, 有川 健太郎, 大山 み乃り, 後藤 薫

    (地独)大阪健康安全基盤研究所　大阪市立環境科学研究所報告 調査・研究年報   ( 73 )   23 - 28   2011.12（ ISSN:0285-5801 ）

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    2010年に食中毒、有症苦情などで検体が搬入された122事件1,624検体について、下痢原性微生物の検出を行った。122事件中73件から下痢原性微生物が検出され、大阪市における細菌あるいはウイルス性食中毒事件として厚生労働省に届けられたのは26件であった。また、73件から検出された病原体の内訳はノロウイルス47件、カンピロバクター14件、サルモネラ属菌4件、下痢原性大腸菌3件、ウェルシュ菌2件と、黄色ブドウ球菌、腸炎ビブリオ、黄色ブドウ球菌/ウェルシュ菌同時検出が各1件であった。2010年は2002年以降の傾向と同様にノロウイルス、カンピロバクター、サルモネラによる食中毒が多く発生したが、特にノロウイルスによる事件の多さが際立っていた。

  • Molecular epidemiology of Mycobacterium tuberculosis and its prospect based on variable number of tandem repeat (VNTR) genotyping--a strategy in Osaka City, Japan.

    Wada T, Hase A

    Kekkaku   85 ( 12 )   845 - 852   2010.12（ ISSN:0022-9776 ）

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  • 2009年に大阪市内の食中毒原因調査において検出された下痢原性微生物

    長谷 篤, 小笠原 準, 中村 寛海, 和田 崇之, 梅田 薫, 入谷 展弘, 久保 英幸, 改田 厚, 阿部 仁一郎, 有川 健太郎, 大山 み乃り, 後藤 薫

    (地独)大阪健康安全基盤研究所　大阪市立環境科学研究所報告 調査・研究年報   ( 72 )   1 - 6   2010.12（ ISSN:0285-5801 ）

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    2009年に市内で食中毒が疑われ当研究所に検体が搬入された事件数は115件で、このうち下痢原生微生物が検出されたのは62件(54%)であった。検出微生物の内訳はノロウイルス41件(66%)、カンピロバクター10件(16%)、サルモネラ属菌6件(10%)、黄色ブドウ球菌3件(5%)、毒素原生大腸菌2件(3%)、ウェルシュ菌1件(1.6%)、O157 1件(1.6%)で、ノロウイルスとカンピロバクターの同時検出例が2件(3%)あった。62件のうち推定原因食品が判明したのは26件(42%)で、主な原因食品は「コース料理」「会席料理」「鶏料理」などであった。原因施設は飲食店が多かった。

  • Phylogeographical particularity of the Mycobacterium tuberculosis Beijing family in South Korea based on international comparison with surrounding countries.

    Kang HY, Wada T, Iwamoto T, Maeda S, Murase Y, Kato S, Kim HJ, Park YK

    Journal of medical microbiology   59 ( Pt 10 )   1191 - 1197   2010.10（ ISSN:0022-2615 ）

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  • Beijing family Mycobacterium tuberculosis isolated from throughout Japan: phylogeny and genetic features

    Maeda S, Wada T, Iwamoto T, Murase Y, Mitarai S, Sugawara I, Kato S

    Int J Tuberc Lung Dis   14 ( 9 )   1201 - 4   2010

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  • 2008年に大阪市内の食中毒原因調査において検出された下痢原性微生物

    長谷 篤, 小笠原 準, 北瀬 照代, 中村 寛海, 和田 崇之, 梅田 薫, 入谷 展弘, 久保 英幸, 改田 厚, 阿部 仁一郎, 藤原 佐美, 後藤 薫

    (地独)大阪健康安全基盤研究所　大阪市立環境科学研究所報告 調査・研究年報   ( 71 )   1 - 6   2009.11（ ISSN:0285-5801 ）

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    2008年に大阪市内の食中毒原因調査で検出した下痢原性微生物について報告した。食中毒が疑われた患者の便と吐物、食品残品、原因と考えられる施設に保存されていた検食と同施設のふきとり水および調理従事者の便と手指のふきとり水を調査の対象とした。食中毒が疑われ検体が搬入された事件数は合計170件で、検体数は1874検体であった。170事件中75件から下痢原性微生物が検出された。170件のうちウイルス性食中毒事件として厚生労働省に届けられた事件数は26件であった。下痢原性微生物が検出された75事件のうち、ノロウイルスが検出されたものが38件、カンピロバクター20件、サルモネラ属菌9件、黄色ブドウ球菌4件、ウェルシュ菌2件、セレウス菌1件、腸管出血性大腸菌O157 1件であった。

  • Population structure dynamics of Mycobacterium tuberculosis Beijing strains during past decades in Japan.

    Iwamoto T, Fujiyama R, Yoshida S, Wada T, Shirai C, Kawakami Y

    Journal of clinical microbiology   47 ( 10 )   3340 - 3343   2009.10（ ISSN:0095-1137 ）

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  • Allelic diversity of variable number of tandem repeats provides phylogenetic clues regarding the Mycobacterium tuberculosis Beijing family.

    Wada T, Iwamoto T

    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases   9 ( 5 )   921 - 926   2009.09（ ISSN:1567-1348 ）

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  • High transmissibility of the modern Beijing Mycobacterium tuberculosis in homeless patients of Japan.

    Wada Takayuki, Fujihara Sami, Shimouchi Akira, Harada Makoto, Ogura Hisashi, Matsumoto Sohkichi, Hase Atsushi

    Tuberculosis (Edinb)   89 ( 4 )   252 - 255   2009.07（ ISSN:1873-281X ）

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    A population-based study of Mycobacterium tuberculosis isolated from homeless tuberculosis patients was performed during 2002-2004 in Osaka City, Japan. The data show that the ancient Beijing subfamily was predominant, whereas clustered isolates based on refined variable number of tandem repeats genotyping (19 loci) mainly belonged to the modern Beijing subfamily, suggesting its increased transmissibility.

    DOI： 10.1016/j.tube.2009.05.007

    PubMed

  • Prevalence and properties of diarrheagenic Escherichia coli among healthy individuals in Osaka City, Japan.

    Fujihara S, Arikawa K, Aota T, Tanaka H, Nakamura H, Wada T, Hase A, Nishikawa Y

    Japanese journal of infectious diseases   62 ( 4 )   318 - 323   2009.07（ ISSN:1344-6304 ）

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  • Genetic diversity of the Mycobacterium tuberculosis Beijing family in East Asia revealed through refined population structure analysis.

    Wada T, Iwamoto T, Maeda S

    FEMS microbiology letters   291 ( 1 )   35 - 43   2009.02（ ISSN:0378-1097 ）

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  • 2007年に大阪市内の食中毒原因調査において検出された下痢原性微生物

    長谷 篤, 小笠原 準, 北瀬 照代, 中村 寛海, 和田 崇之, 梅田 薫, 入谷 展弘, 久保 英幸, 改田 厚, 阿部 仁一郎, 後藤 薫, 石井 營次

    (地独)大阪健康安全基盤研究所　大阪市立環境科学研究所報告 調査・研究年報   ( 70 )   9 - 14   2008.11（ ISSN:0285-5801 ）

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    2007年に大阪市内の食中毒原因調査において検出された下痢原性微生物について報告した。食中毒が疑われた患者の便と吐物、食品残品、原因と考えられる施設(患者宅を含む)に保存されていた検食と同施設のふきとり水および調理従事者の便と手指のふきとり水を調査の対象とした。検体が搬入された事件数は合計139件で、患者および関係者の検便および吐物1482検体、食品、ふき取りなどが892検体であった。139事件中66件から下痢原性微生物を検出した。139件のうち大阪市における細菌あるいはウイルス性食中毒事件として厚生労働省に届けられた事件数は33件であった。下痢原性微生物が検出された66事件の内訳は、ノロウイルスが29件で最も多く、以下、カンピロバクター19件、サルモネラ11件、黄色ブドウ球菌4件、腸炎ビブリオ2件、ウェルシュ菌1件であった。

  • Population structure analysis of the Mycobacterium tuberculosis Beijing family indicates an association between certain sublineages and multidrug resistance.

    Iwamoto T, Yoshida S, Suzuki K, Wada T

    Antimicrobial agents and chemotherapy   52 ( 10 )   3805 - 3809   2008.10（ ISSN:0066-4804 ）

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  • 2006年に大阪市内の食中毒原因調査において検出された下痢原性微生物と主な事件の概要

    長谷 篤, 小笠原 準, 北瀬 照代, 中村 寛海, 和田 崇之, 梅田 薫, 入谷 展弘, 久保 英幸, 改田 厚, 阿部 仁一郎, 後藤 薫, 石井 營次

    (地独)大阪健康安全基盤研究所　大阪市立環境科学研究所報告 調査・研究年報   ( 69 )   1 - 6   2007.11（ ISSN:0285-5801 ）

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    大阪市内で食中毒が疑われ検体が搬入された事件について毎年まとめ、その原因病原体と疫学情報を基に感染源を明らかにした。2006年に大阪市内で食中毒が疑われた202事件について下痢原性微生物の検出を行った。下痢原性微生物が検出された121事件(59.9%)のうち、ノロウイルスが検出されたものが94件(77.7%)、カンピロバクター12件(9.9%)、サルモネラ7件(5.8%)、腸炎ビブリオ3件(2.5%)、セレウス菌2件、黄色ブドウ球菌1件、病原大腸菌1件、エロモナス1件であった。また、市内の一ホテルにおいて患者数160例のノロウイルスによる大規模食中毒事件が発生した。

  • Evaluation of variable numbers of tandem repeat as molecular epidemiological markers of Mycobacterium tuberculosis in Japan.

    Wada T, Maeda S, Hase A, Kobayashi K

    Journal of medical microbiology   56 ( Pt 8 )   1052 - 1057   2007.08（ ISSN:0022-2615 ）

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  • 2005年に大阪市内の食中毒原因調査において検出された下痢原性微生物と主な事件の概要

    長谷 篤, 小笠原 準, 北瀬 照代, 中村 寛海, 和田 崇之, 梅田 薫, 入谷 展弘, 久保 英幸, 改田 厚, 阿部 仁一郎, 後藤 薫, 村上 司, 石井 營次

    (地独)大阪健康安全基盤研究所　大阪市立環境科学研究所報告 調査・研究年報   ( 68 )   13 - 18   2007.01（ ISSN:0285-5801 ）

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    2005年に大阪市内で食中毒が疑われた168事件について、その原因病原体と疫学情報を基に感染源を調査した。下痢原性微生物が検出された76事件(45.2%)のうち、ノロウイルスが検出されたものが51件(67.1%)、キャンピロバクター9件(11.9%)、サルモネラ6件(7.9%)、ウェルシュ菌4件(5.3%)、黄色ブドウ球菌2件(2.6%)、セレウス菌1件(1.3%)、腸炎ビブリオ1件(1.3%)、病原大腸菌1件(1.3%)、サルモネラ/キャンピロバクター同時検出が1件(1.3%)あった。また、患者数190例のサルモネラ・エンテリティディス集団食中毒が発生した。

  • 2004年に大阪市内の食中毒原因調査において検出された下痢原性微生物

    長谷 篤, 小笠原 準, 北瀬 照代, 中村 寛海, 和田 崇之, 中田 薫, 入谷 展弘, 久保 英幸, 改田 厚, 阿部 仁一郎, 村上 司, 春木 孝祐, 石井 営次

    (地独)大阪健康安全基盤研究所　大阪市立環境科学研究所報告 調査・研究年報   ( 67 )   9 - 14   2005.11（ ISSN:0285-5801 ）

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    2004年に大阪市内で食中毒が疑われた事件について,その原因病原体と疫学情報を基に感染源を調査した.食中毒が疑われた患者の便と吐物,食品残品,原因と考えられる施設に保存されていた検食と同施設のふきとり水および調理従事者の便と手指のふきとり水を調査の対象とした.183事件について下痢原性微生物の検出を行った.下痢原性微生物が検出された71事件中,ノロウイルス47件,サルモネラ10件,キャンピロバクター7件,腸炎ビブリオ3件,黄色ブドウ球菌1件,ウェルシュ菌1件,セレウス菌1件,黄色ブドウ球菌・セレウス菌同時検出1件あった

  • 大阪市内における結核蔓延状況の分子疫学的解析および薬剤耐性結核の出現機序に関する研究

    長谷 篤, 和田 崇之

    (地独)大阪健康安全基盤研究所　大阪市立環境科学研究所報告 調査・研究年報   ( 67 )   65 - 66   2005.11（ ISSN:0285-5801 ）

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    大阪市内で分離した結核菌の分子疫学的解析,薬剤耐性結核の出現に対する危機管理に関して,従来法に代わる新手法についてその有用性を検討した.結核菌株251株についてMIRU-VNTR法による分子疫学的解析を行った.MIRU-VNTR法は患者接触調査などと合わせた感染源調査では充分な威力を発揮することが予想された.ただし,MIRU-VNTRでの分離が難しいパターンの存在が明らかになった.対照プローブと他のプローブの蛍光強度を比較することによって,目的遺伝子内の変異を検出できることが明らかとなった.さらに,これらのプローブ濃度を調整することで,一定の蛍光強度に至るために必要なPCRサイクル数を比較するだけで突然変異の有無が判定できるシステムとして完成させた

  • Dual-probe assay for rapid detection of drug-resistant Mycobacterium tuberculosis by real-time PCR

    T Wada, S Maeda, A Tamaru, S Imai, A Hase, K Kobayashi

    AMER SOC MICROBIOLOGY　JOURNAL OF CLINICAL MICROBIOLOGY   42 ( 11 )   5277 - 5285   2004.11（ ISSN:0095-1137 ）

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    Mutations in particular nucleotides of genes coding for drug targets or drug-converting enzymes lead to drug resistance in Mycobacterium tuberculosis. For rapid detection of drug-resistant M. tuberculosis in clinical specimens, a simple and applicable method is needed. Eight TaqMan minor groove binder (MGB) probes, which discriminate one-base mismatches, were designed (dual-probe assay with four reaction tubes). The target of six MGB probes was the rpoB gene, which is involved in rifampin resistance; five probes were designed to detect for mutation sites within an 81-bp hot spot of the rpoB gene, and one probe was designed as a tuberculosis (TB) control outside the rpoB gene hot-spot. We also designed probes to examine codon 315 of katG and codon 306 of embB for mutations associated with resistance to isoniazid and ethambutol, respectively. Our system was M. tuberculosis complex specific, because neither nontuberculous mycobacteria nor bacteria other than mycobacteria reacted with the system. Detection limits in direct and preamplified analyses were 250 and 10 fg of genomic DNA, respectively. The system could detect mutations of the rpoB, katG, and embB genes in DNAs extracted from 45 laboratory strains and from sputum samples of 27 patients with pulmonary TB. This system was much faster (3 h from DNA preparation) than conventional drug susceptibility testing (3 weeks). Results from the dual-MGB-probe assay were consistent with DNA sequencing. Because the dual-probe assay system is simple, rapid, and accurate, it can be applied to detect drug-resistant M. tuberculosis in clinical laboratories.

    DOI： 10.1128/JCM.42.11.5277-5285.2004

    PubMed

  • Extracellular mycobacterial DNA-binding protein 1 participates in mycobacterium-lung epithelial cell interaction through hyaluronic acid

    K Aoki, S Matsumoto, Y Hirayama, T Wada, Y Ozeki, M Niki, P Domenech, K Umemori, S Yamamoto, A Mineda, M Matsumoto, K Kobayashi

    AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC　JOURNAL OF BIOLOGICAL CHEMISTRY   279 ( 38 )   39798 - 39806   2004.09（ ISSN:0021-9258 ）

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    Mycobacterium tuberculosis infects not only host macrophages but also nonprofessional phagocytes, such as alveolar epithelial cells. Glycosaminoglycans (GAGs) are considered as the component of mycobacterial adherence to epithelial cells. Here we show that extracellularly occurring mycobacterial DNA-binding protein 1 (MDP1) promotes mycobacterial infection to A549 human lung epithelial cells through hyaluronic acid ( HA). Both surface plasmon resonance analysis and enzyme-linked immunosorbent assay revealed that MDP1 bound to HA, heparin, and chondroitin sulfate. Utilizing synthetic peptides, we next defined heparin-binding site of 20 amino acids from 31 to 50 of MDP1, which is responsible for the specific DNA-binding site of MDP1. MDP1 bound to A549 cells, and exogenous DNA and HA interfered with the interaction. The binding was also abolished by treatment of A549 cells with hyaluronidase, suggesting that HA participates in the MDP1-A549 cell interaction. Adherence of bacillus Calmette-Guerin ( BCG) and M. tuberculosis to A549 cells was inhibited by addition of HA, DNA, and anti-MDP1 antibody, showing that MDP1 participates in the interaction between mycobacteria-alveolar epithelial cells. Simultaneous treatment of intratracheal BCG-infected mice with HA reduced the growth of BCG in vivo. Taken together, theses results suggest that HA participates in Mycobacterium-lung epithelium interaction and has potential for therapeutic and prophylactic interventions in mycobacterial infection.

    DOI： 10.1074/jbc.M402677200

    PubMed

  • Diarrheagenic Escherichia coli isolated from stools of sporadic cases of diarrheal illness in Osaka City, Japan between 1997 and 2000: Prevalence of enteroaggregative E-coli heat-stable enterotoxin 1 gene-possessing E-coli

    Y Nishikawa, ZJ Zhou, A Hase, J Ogasawara, T Kitase, N Abe, H Nakamura, T Wada, E Ishii, K Haruki

    NATL INST INFECTIOUS DISEASES　JAPANESE JOURNAL OF INFECTIOUS DISEASES   55 ( 6 )   183 - 190   2002.12（ ISSN:1344-6304 ）

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    Diarrheagenic Escherichia coli (DEC) represents an elusive target, since they are not easily distinguished from fecal coliforms. To clarify if DEC are prevalent among sporadic cases of diarrheal illness in Osaka City Japan, diarrheal specimens were examined for E. coli that were enterohemorrhagic (EHEC), enteiopathogenic (EPEC), enterotoxigenic (ETEC), enteroinvasive (EIEC), or enteroaggregative (EAggEC). EAST1EC, a strain of E. coli that does not possess any diarrheagenic characteristics except the EAggEC heat-stable toxin I (EAST1) gene, was also included as a possible DEC. A total of 924 specimens were examined between July 1997 and March 2000. DEC and Salmonella were isolated from 7.3% (67/924) and 6.8% (63/924) of specimens, respectively. DEC was therefore as prevalent as Salmonella among sporadic cases. The 67 strains were composed of 17 EPEC (26%), 10 EHEC (15%), four ETEC (6%), 13 EAggEC (20%), and 23 EAST1EC (35%), including two. strains of EAST1EC 0 166:H15. Although PCR and tissue culture adhesion tests were useful to detect DEC, the effectiveness of serotyping was limited: only 40 strains (17.5%) out of 229 isolates that had been assumed to be enterovirulent on the basis of their 0 antigen were recognized to be diarrheagenic. In conclusion, not only EHEC but also the other subgroups of DEC, including EAST1EC, seem to play an important role, in causing sporadic diarrheal illnesses. Methods to detect and unified criteria to identify various kinds of DEC are strongly desired.

    PubMed

  • Type XXVI collagen, a new member of the collagen family, is specifically expressed in the testis and ovary

    K Sato, K Yomogida, T Wada, T Yorihuzi, Y Nishimune, N Hosokawa, K Nagata

    AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC　JOURNAL OF BIOLOGICAL CHEMISTRY   277 ( 40 )   37678 - 37684   2002.10（ ISSN:0021-9258 ）

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    HSP47 is a collagen-specific molecular chaperone that specifically recognizes and binds to the triple helical domain of various types of collagens. Here we report the cloning of the entire coding region of a novel collagen-like protein by yeast two-hybrid screening of a 17.5-day whole mouse embryo cDNA library using HSP47 as a bait. The cDNA of this protein and its deduced amino acid sequence are 2,690 bp and 438 amino acids long, respectively. The protein contains two clusters of Gly-X-Y collagenous repeats and three noncollagenous domains. Northern blot analysis showed that its mRNA is specifically expressed in the testis and ovary in adult tissues and that expression in these tissues is highest in the neonate. Biochemical characterization of this protein revealed that its proline residues are hydroxylated, it undergoes N-linked glycosylation, it forms trimers, and it is secreted in vitro. Immunohistochemical studies showed that the myoid cells and the pre-theca cells synthesized it in the testis and ovary, respectively, resulting in the accumulation of this protein in the extracellular spaces of these organs. these observations suggest that this protein is a new member of the collagen protein family. We thus designated this protein as type XXVI collagen.

    DOI： 10.1074/jbc.M205347200

    PubMed

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Books and Other Publications

  • BCG –Vaccine and Adjuvant–

    Wada T, Iwamoto T（ Role： Sole author ,  Genetic diversity of Mycobacterium tuberculosis and its impact on adaptive evolution, vaccination efficacy, and population dynamics.）

    JATA Press  2011 

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    Book type：Scholarly book

MISC

  • 山形県における結核菌ゲノム解析を用いた結核分子疫学調査

    瀬戸 順次, 和田 崇之, 村瀬 良朗, 三瓶 美香, 下村 佳子, 細谷 真紀子, 水田 克巳, 御手洗 聡, 阿彦 忠之

    感染症学雑誌   97 ( 1 )   6 - 17   2023.01（ ISSN:0387-5911 ） （ eISSN:1884-569X ）

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    背景:本邦では結核菌ゲノム解析と実地疫学を組み合わせた積極的結核疫学調査の有用性評価は行われていない.方法:2009～2020年の山形県における反復配列多型(VNTR)分析に基づいた網羅的な結核分子疫学調査結果により見出されたクラスターのうち,2020年の結核患者8人を含む8クラスター(19人)を対象に結核菌ゲノム比較を実施し,保健所の実地疫学調査結果と併せて評価した.結果:結核菌ゲノム比較の結果,近縁株(一塩基多型5ヶ所以内,もしくは6～12ヶ所かつ患者間に疫学的関連性を確認)は19株中9株(47.4%)に留まり,半数の4クラスターにのみ近縁株が含まれた.また,近縁株患者9人中6人(66.7%)で患者間の疫学的関連性が見出されていた一方,分離された菌が患者間の最近の結核感染伝播を示唆しない非近縁株(一塩基多型13ヶ所以上)であった10人は,全例が疫学的関連性不明であった(p<0.01).結論:高精細な識別能を有する結核菌ゲノム解析は,VNTR分析によりクラスターを形成した菌株から,患者間の疫学的関連性を強く示唆する近縁株を選別することができた.その特長は,保健所の感染伝播経路調査に要する人的・時間的資源を必要な結核患者に集中させることを可能とし,効率的かつ高精度の積極的結核疫学調査に貢献するものと考えられた.(著者抄録)

    Other URL： https://search.jamas.or.jp/default/link?pub_year=2023&ichushi_jid=J00276&link_issn=&doc_id=20230130440002&doc_link_id=10.11150%2Fkansenshogakuzasshi.e22021&url=https%3A%2F%2Fdoi.org%2F10.11150%2Fkansenshogakuzasshi.e22021&type=J-STAGE&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00007_3.gif

  • 比較ゲノム解析による集団感染事例の感染伝播の検討

    山本 香織, 蒲田 脩圭里, 米田 佳美, 永石 真知子, 小向 潤, 吉田 英樹, 有川 健太郎, 岩本 朋忠, 村瀬 良朗, 御手洗 聡, 和田 崇之

    結核   96 ( 4 )   103 - 103   2021.06（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

  • Genomic DNA extraction from Mycobacterium avium

    Tetsuya Komatsu, Kenji Ohya, Justice Opare Odoi, Shota Suganuma, Kotaro Sawai, Takayuki Wada, Tomotada Iwamoto, Fumito Maruyama

    protocols.io   2021.05

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  • 世界の結核研究の動向（１４）ゲノム科学の奔流と結核分子疫学

    和田崇之

    複十字   388   20 - 21   2019.09

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  • 結核に罹患したボルネオゾウの原因菌はヒト結核菌ではなくMycobacterium capraeだった

    吉田 志緒美, 菅 里美, 石川 智史, 向井 康彦, 露口 一成, 井上 義一, 山本 太郎, 和田 崇之

    日本細菌学雑誌   74 ( 1 )   44 - 44   2019.03（ ISSN:0021-4930 ）

  • 非同義置換一塩基多型を利用した結核菌M株の機能因子の探索

    前田 伸司, 和田 崇之, 藤原 永年

    日本細菌学雑誌   74 ( 1 )   142 - 142   2019.03（ ISSN:0021-4930 ） （ eISSN:1882-4110 ）

  • 大阪市における社会経済的指標を用いた結核発生状況の地域差

    山本 香織, 中谷 友樹, 竹内 昌平, 小向 潤, 青木 理恵, 松本 健二, 吉田 英樹, 山本 太郎, 和田 崇之

    日本公衆衛生学会総会抄録集   77回   452 - 452   2018.10（ ISSN:1347-8060 ）

  • 同一遺伝子型多剤耐性結核菌V02群の遺伝子変異

    田丸亜貴, 岩本朋忠, 和田崇之

    結核   93 ( 4 )   289 - 289   2018.04（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

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  • 大阪市における結核発生状況の地域差

    山本 香織, 和田 崇之, 小向 潤, 清水 直子, 青木 理恵, 長谷 篤, 松本 健二, 吉田 英樹, 山本 太郎

    結核   93 ( 4 )   346 - 346   2018.04（ ISSN:0022-9776 ）

  • BCGの増殖率促進に関与する遺伝子の調査(Investigation of genes involved in promoting the growth rate of BCG)

    竜門 亜矢子, 中山 真彰, 和田 崇之, 橘 理人, 阿戸 学, 中島 千絵, 鈴木 定彦, 小崎 弘貴, 大原 直也

    日本細菌学雑誌   73 ( 1 )   68 - 68   2018.02（ ISSN:0021-4930 ）

  • BCG接種後6ヵ月で第1趾骨髄炎を呈した1歳男児例

    佐々木 恭介, 鈴木 重雄, 石橋 直尚, 保科 めぐみ, 望月 いづみ, 竹田 悠佳, 三島 博, 朝熊 英也, 内海 康文, 吉田 志緒美, 和田 崇之

    日本小児科学会雑誌   122 ( 1 )   89 - 89   2018.01（ ISSN:0001-6543 ）

  • 大阪市あいりん地域における結核の感染伝播状況(2006-2015)

    山本 香織, 和田 崇之, 小向 潤, 宇田 瑛子, 清水 直子, 松本 健二, 下内 昭, 山本 太郎

    日本公衆衛生学会総会抄録集   76回   572 - 572   2017.10（ ISSN:1347-8060 ）

  • 上皮細胞に対する腸管毒素原性大腸菌O169:H41の特異な接着性に寄与する新規付着因子 Reviewed

    鄭 冬明, 坂 瑛里香, 大森 裕子, 池崎 沙耶香, 中臺 枝里子, 和田 崇之, 工藤 由起子, 西川 禎一

    日本栄養・食糧学会大会講演要旨集   71回   243 - 243   2017.04

  • ヒト，動物，環境をとりまく非結核性抗酸菌の浸淫状況と宿主適応．

    和田崇之, 吉田志緒美, 柳井徳磨

    日本臨床微生物学雑誌   27 ( 3 )   1 - 10   2017.03

  • 多様な研究分野から見えてくる抗酸菌学の魅力 アジアにおけるOne Healthに基づいた抗酸菌症のサーベイランスネットワークについて

    柳井 徳磨, 吉田 志緒美, 和田 崇之

    日本細菌学雑誌   72 ( 1 )   24 - 24   2017.02（ ISSN:0021-4930 ）

  • 大阪市内の40歳未満結核患者から分離された結核菌株の分子疫学解析

    山本 香織, 和田 崇之, 小向 潤, 八木 沙保里, 宇田 瑛子, 清水 直子, 齊藤 和美, 青木 理恵, 松本 健二, 下内 昭, 山本 太郎

    日本公衆衛生学会総会抄録集   75回   559 - 559   2016.10（ ISSN:1347-8060 ）

  • 結核患者情報は菌株の遺伝多型(VNTR型別)解析に基づく感染源推定に影響を与えるか?

    和田 崇之, 山本 香織, 山本 太郎, 阿彦 忠之, 瀬戸 順次, 林 哲也, 小椋 義俊

    日本公衆衛生学会総会抄録集   75回   300 - 300   2016.10（ ISSN:1347-8060 ）

  • 大阪市あいりん地域における結核菌伝播状況解析(2006〜2014)

    山本 香織, 和田 崇之, 小向 潤, 長谷 篤, 松本 健二, 下内 昭, 山本 太郎

    結核   91 ( 3 )   363 - 363   2016.03（ ISSN:0022-9776 ）

  • 次世代シーケンサーを用いたBCG Tokyo 172のシードロットおよび市販ロットにおけるヘテロ変異検出

    和田 崇之, 岩本 朋忠, 前田 伸司, 山本 太郎, 山本 三郎, 大原 直也, 中川 一路, 丸山 史人

    結核   91 ( 3 )   326 - 326   2016.03（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

  • 播種性病態を引き起こした新世界ザル由来Mycobacterium kansasii株の遺伝子解析及び病原性評価

    和田 崇之, 吉田 志緒美, 露口 一成, 山本 太郎, 柳井 徳磨, 横田 伸一, 服部 正策

    感染症学雑誌   90 ( 臨増 )   271 - 271   2016.03（ ISSN:0387-5911 ）

  • 滅菌水供給装置の汚染が原因と考えられたMycobacterium chimaeraの疑似アウトブレイク

    吉田 志緒美, 東 桃代, 露口 一成, 鈴木 克洋, 井上 義一, 和田 崇之, 山本 太郎, 林 清二

    結核   91 ( 3 )   310 - 310   2016.03（ ISSN:0022-9776 ）

  • ETEC O169:H41株の病毒性プラスミドと新規のコロニー化因子の完全長DNAシークエンス分析(Complete DNA sequence of the ETEC o169:H41 virulence plasmid and the novel colonization factor) Reviewed

    鄭 冬明, 坂 瑛里香, 池崎 沙耶加, 中台 枝里子, 和田 崇之, 輪島 丈明, 濱端 崇, 堀口 安彦, 西川 禎一

    日本細菌学雑誌   71 ( 1 )   122 - 122   2016.02（ ISSN:0021-4930 ） （ eISSN:1882-4110 ）

  • 次世代シーケンサーによるBCGワクチンのシードロットと市販ロットにおけるヘテロ変異の検出

    和田 崇之, 丸山 史人, 岩本 朋忠, 山本 太郎, 中川 一路, 山本 三郎, 大原 直也

    日本細菌学雑誌   71 ( 1 )   134 - 134   2016.02（ ISSN:0021-4930 ） （ eISSN:1882-4110 ）

  • 大阪市あいりん地域における結核菌分子疫学調査

    山本 香織, 和田 崇之, 小向 潤, 松本 健二, 下内 昭, 山本 太郎

    日本公衆衛生学会総会抄録集   74回   456 - 456   2015.10（ ISSN:1347-8060 ）

  • 抗酸菌属に潜む分類学と臨床現場の乖離

    和田崇之, 吉田志緒美, 山本太郎

    生物の科学「遺伝」   69 ( 5 )   415 - 420   2015.09

  • ベトナムハノイ地区で分離された結核菌の分子疫学解析

    前田 伸司, 和田 崇之, 櫻田 紳策, 小林 信之, 慶長 直人

    結核   90 ( 2 )   348 - 348   2015.02（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

  • 進化とエコヘルス.

    山本太郎, 和田崇之, 市川智生

    医学の歩み   250 ( 3 )   230 - 234   2014.07

  • 1960〜80年代における結核菌の薬剤耐性状況の推察 抗結核薬の変遷とその影響について

    吉田 志緒美, 露口 一成, 岡田 全司, 鈴木 克洋, 林 洋二, 飛永 祥平, 松本 充生, 山本 太郎, 和田 崇之

    結核   89 ( 5 )   584 - 584   2014.05（ ISSN:0022-9776 ）

  • 1960〜70年代における結核菌の薬剤耐性状況の推察 抗結核薬の変遷とその影響について

    吉田 志緒美, 露口 一成, 鈴木 克洋, 富田 元久, 岡田 全司, 和田 崇之, 山本 太郎, 林 清二

    結核   89 ( 3 )   471 - 471   2014.03（ ISSN:0022-9776 ）

  • M株の広域的分離の原因究明―比較ゲノム解析に基づく「結核ゲノム疫学」の導入

    和田崇之, 岩本朋忠, 瀬戸順次, 田丸亜貴, 長谷篤, 前田伸司, 阿彦忠之, 山本太郎

    結核   89 ( 3 )   465 - 465   2014.03（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

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  • 結核分子疫学体制の今：克服すべき課題と展望について.

    和田崇之

    公衆衛生   76 ( 1 )   2 - 3   2014.01

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  • 歯肉結核の1例

    香西 博之, 大串 文隆, 篠原 勉, 畠山 暢生, 町田 久典, 岡野 義夫, 中野 万有里, 飛梅 亮, 吉田 志緒美, 和田 崇之

    結核   88 ( 5 )   519 - 520   2013.05（ ISSN:0022-9776 ）

  • 結核菌北京型1,054株を用いた遺伝的系統解析法の評価(Re-evaluation of epidemiological methods for genotyping on 1,054 M. tuberculosis Beijing strains)

    中西 典子, 和田 崇之, 有川 健太郎, 岩本 朋忠

    日本細菌学雑誌   68 ( 1 )   126 - 126   2013.02（ ISSN:0021-4930 ） （ eISSN:1882-4110 ）

  • 遺伝子型別解析から浮かび上がる結核菌地域蔓延株の可能性

    吉田 志緒美, 露口 一成, 鈴木 克洋, 岡田 全司, 和田 崇之, 岩本 朋忠

    感染症学雑誌   86 ( 3 )   355 - 356   2012.05（ ISSN:0387-5911 ）

  • 半世紀前における結核患者由来病理組織標本からの結核菌DNA抽出の試み

    吉田 志緒美, 鈴木 克洋, 露口 一成, 岡田 全司, 林 清二, 岩本 朋忠, 和田 崇之

    結核   87 ( 3 )   232 - 232   2012.03（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

  • 集団遺伝学的解析による北京型結核菌の地域間比較

    横山 栄二, 蜂巣 友嗣, 岸田 一則, 岩本 朋忠, 中西 典子, 有川 健太郎, 和田 崇之, 長谷 篤, 瀬戸 順次, 阿彦 忠之

    結核   87 ( 3 )   230 - 230   2012.03（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

  • ゲノム比較に基づいた結核菌臨床分離株の遺伝的多様性包囲網の構築

    和田 崇之, 岩本 朋忠, 長谷 篤, 前田 伸司

    日本細菌学雑誌   67 ( 1 )   82 - 82   2012.02（ ISSN:0021-4930 ） （ eISSN:1882-4110 ）

  • 実験研究者のためのバイオインフォマティックス 結核菌分子疫学解析からのデータマインニングを目指したゲノム解析

    岩本 朋忠, 和田 崇之, 前田 伸司

    日本細菌学雑誌   67 ( 1 )   66 - 66   2012.02（ ISSN:0021-4930 ） （ eISSN:1882-4110 ）

  • ペルーにおける結核菌北京型株の遺伝的多様性

    有川 健太郎, 中西 典子, 和田 崇之, 岩本 朋忠

    日本細菌学雑誌   67 ( 1 )   83 - 83   2012.02（ ISSN:0021-4930 ） （ eISSN:1882-4110 ）

  • 結核患者由来病理組織標本からの結核菌DNA抽出と遺伝子型別解析の試み

    吉田 志緒美, 江口 克之, 岩本 朋忠, 山本 太郎, 和田 崇之, 北市 正則, 鈴木 克洋, 露口 一成, 岡田 全司, 林 清二

    日本細菌学雑誌   67 ( 1 )   126 - 126   2012.02（ ISSN:0021-4930 ） （ eISSN:1882-4110 ）

  • 近縁ゲノム比較からの発見 ゲノム比較に基づいた結核菌臨床分離株の遺伝的多様性包囲網の構築

    和田 崇之, 岩本 朋忠, 長谷 篤, 前田 伸司

    日本細菌学雑誌   67 ( 1 )   61 - 61   2012.02（ ISSN:0021-4930 ） （ eISSN:1882-4110 ）

  • ゲノム配列にもとづく結核菌群の発生と進化の理解．

    和田崇之, 前田伸司

    化学療法の領域   27 ( 6 )   25 - 31   2011.06

  • VNTR解析活用における課題について

    吉田志緒美, 露口一成, 鈴木克洋, 岡田全司, 林清二, 和田崇之, 岩本朋忠, 田丸亜貴

    結核   86 ( 3 )   319 - 319   2011.03（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

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  • 本邦結核菌の遺伝的多元性と高齢者結核の関与

    岩本朋忠, 中西典子, 吉田志緒美, 和田崇之, 田丸亜貴, 藤山理世, 白井千香

    結核   86 ( 3 )   330 - 330   2011.03（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

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  • 患者年齢構成の異なる地域間での結核菌地域分子疫学比較

    田丸亜貴, 和田崇之, 岩本朋忠, 吉田志緒美, 野尻孝子

    結核   86 ( 3 )   376 - 376   2011.03（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

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  • ホームレス結核患者由来株のVNTR型別解析:多発性大規模伝播と未知の伝播経路推定

    和田崇之, 小向潤, 有馬和代, 團野桂, 松本健二, 吉田英樹, 加藤仁一, 岩本朋忠, 田丸亜貴, 吉田志緒美, 下内昭, 長谷篤

    結核   86 ( 3 )   318 - 318   2011.03（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

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  • 高齢者結核患者から高頻度に分離される結核菌株群の集団特性と遺伝学的特徴

    和田 崇之, 岩本 朋忠, 長谷 篤, 前田 伸司

    結核   86 ( 3 )   329 - 329   2011.03（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

  • マイクロアレイCGH法による本邦由来北京型結核菌のゲノム個性解析

    和田 崇之, 岩本 朋忠, 吉田 志緒美, 長谷 篤, 前田 伸司

    結核   85 ( 4 )   404 - 404   2010.04（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

  • 大阪府における多発性広域感染株について

    田丸亜貴, 和田崇之, 長谷篤, 岩本朋忠, 吉田志緒美, 前田伸司, 松本智成

    結核   85 ( 4 )   411 - 411   2010.04（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

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  • 多発性大規模感染株による多剤耐性化事例について

    和田崇之, 團野桂, 松本智成, 田丸亜貴, 門林順子, 松本健二, 岩本朋忠, 吉田志緒美, 前田伸司, 長谷篤

    結核   85 ( 4 )   403 - 403   2010.04（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

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  • 多発性大規模感染に関連する結核菌株による集団事例の疫学情報

    和田崇之, 三宅由起, 加藤仁一, 吉田英樹, 岩本朋忠, 田丸亜貴, 松本智成, 吉田志緒美, 前田伸司, 下内昭, 長谷篤

    結核   85 ( 4 )   404 - 404   2010.04（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

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  • 分子疫学情報の蓄積から示唆される多発性大規模感染事例の存在とその検証

    岩本朋忠, 藤山理世, 白井千香, 前田伸司, 松本智成, 和田崇之, 田丸亜貴, 吉田志緒美, 河上靖登

    結核   85 ( 4 )   411 - 411   2010.04（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

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  • 国内結核菌を効率よく型別するための標準反復配列多型(VNTR)分析法

    前田 伸司, 和田 崇之, 岩本 朋忠

    日本細菌学雑誌   65 ( 1 )   201 - 201   2010.02（ ISSN:0021-4930 ） （ eISSN:1882-4110 ）

  • リファンピシンとリファブチンの抗抗酸菌活性ならびにrpoB遺伝子変異の関係

    吉田 志緒美, 鈴木 克洋, 露口 一成, 岡田 全司, 富田 元久, 和田 崇之, 岩本 朋忠, 坂谷 光則

    結核   84 ( 5 )   441 - 441   2009.05（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

  • 地域的に限局されないVNTR型別結核菌の同定と広範的分子疫学へのアプローチ

    和田 崇之, 前田 伸司, 岩本 朋忠, 長谷 篤

    結核   84 ( 5 )   386 - 386   2009.05（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

  • 抗酸菌感染症研究における新たな視点 結核ゲノム疫学の創出を目的とした結核菌臨床分離株の全ゲノム比較解析

    和田 崇之, 岩本 朋忠, 吉田 志緒美, 前田 伸司, 長谷 篤

    日本細菌学雑誌   64 ( 1 )   93 - 93   2009.02（ ISSN:0021-4930 ） （ eISSN:1882-4110 ）

  • これ知っとこう！⑥「北京株」.

    和田崇之, 前田伸司

    保健師・看護師の結核展望   47 ( 1 )   86 - 89   2009.01

  • 入院期間中に薬剤感受性が変化した肺結核症の1症例における分子遺伝子学的検討

    吉田 志緒美, 鈴木 克洋, 岩本 朋忠, 和田 崇之, 露口 一成, 岡田 全司, 坂谷 光則

    結核   83 ( 3 )   277 - 277   2008.03（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

  • 抗酸菌の薬剤感受性におけるMDP1の調節機構の解析

    仁木 誠, 吉村 満美子, 和田 崇之, 小林 和夫, 松本 壮吉

    結核   83 ( 3 )   342 - 342   2008.03（ ISSN:0022-9776 ）

  • 北京型結核菌の遺伝系統別分類とサブグループ間での多剤耐性結核菌出現頻度の比較

    岩本 朋忠, 吉田 志緒美, 鈴木 克洋, 和田 崇之

    結核   83 ( 3 )   317 - 317   2008.03（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

  • 北京型結核菌のサブグループにおける遺伝的特徴 VNTR多型解析を中心として

    和田 崇之, 岩本 朋忠, 吉田 志緒美, 長谷 篤

    結核   83 ( 3 )   314 - 314   2008.03（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

  • これ知っとこう！⑤「VNTR」.

    和田崇之, 前田伸司

    保健師・看護師の結核展望   46 ( 2 )   86 - 87   2008.02

  • 抗酸菌の遺伝子発現および薬剤感受性におけるMDP1の役割

    仁木 誠, 吉村 満美子, 和田 崇之, 小林 和夫, 松本 壮吉

    日本細菌学雑誌   63 ( 1 )   183 - 183   2008.02（ ISSN:0021-4930 ）

  • 結核菌北京型ファミリーのグループ内進化にともなう多剤耐性獲得能力の変化

    岩本 朋忠, 和田 崇之, 吉田 志緒美

    日本細菌学雑誌   63 ( 1 )   139 - 139   2008.02（ ISSN:0021-4930 ） （ eISSN:1882-4110 ）

  • 結核菌北京型ファミリー内における微小分子進化とミニサテライト多型解析

    和田 崇之, 岩本 朋忠, 吉田 志緒美, 長谷 篤

    日本細菌学雑誌   63 ( 1 )   175 - 175   2008.02（ ISSN:0021-4930 ） （ eISSN:1882-4110 ）

  • 抗酸菌の分子疫学法.

    和田崇之, 前田伸司

    呼吸器科   13 ( 1 )   92 - 98   2008.01

  • 抗酸菌の分子疫学解析とその応用.

    前田伸司, 和田崇之

    Medical Technology   36 ( 2 )   170 - 175   2008.01

  • ヒアルロン酸の抗酸菌増殖に対する作用

    平山 幸雄, 吉村 満美子, 仁木 誠, 松本 壮吉, 尾関 百合子, 菅原 勇, 青木 俊明, 和田 崇之, 西内 由紀子, 小林 和夫

    結核   82 ( 4 )   404 - 404   2007.04（ ISSN:0022-9776 ）

  • 日本国内の北京型結核菌に見られる遺伝的特異性

    和田 崇之, 長谷 篤, 前田 伸司, Shi Ruiru, 菅原 勇, 松本 壮吉, 岩本 朋忠

    結核   82 ( 4 )   372 - 372   2007.04（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

  • 新提案VNTR setを用いたMinimum Spanning Treeとスポリゴタイピングによる結核菌伝播についての考察

    岩本 朋忠, 園部 俊明, 和田 崇之, 吉田 志緒美, 鈴木 克洋, 藤山 理世, 田中 賀子, 河上 靖登, 白井 千香

    結核   82 ( 4 )   370 - 370   2007.04（ ISSN:0022-9776 ） （ eISSN:1884-2410 ）

  • 抗酸菌の蛋白質発現と薬剤抵抗性におけるMDP1の役割

    仁木 誠, 吉村 満美子, 平山 幸雄, 松本 壮吉, 和田 崇之, 小林 和夫

    結核   82 ( 4 )   403 - 403   2007.04（ ISSN:0022-9776 ）

  • 結核菌糖脂質の合成制御におけるmycobacterial DNA-binding protein 1(MDP1)の役割

    松本 壮吉, 藤原 永年, 吉村 満美子, 尾関 百合子, 西内 由紀子, 和田 崇之, 小林 和夫

    結核   82 ( 4 )   402 - 402   2007.04（ ISSN:0022-9776 ）

  • 薬剤耐性結核菌の迅速検出法.

    和田崇之

    生活衛生   51 ( 3 )   141 - 148   2007.03

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  • 休眠期抗酸菌の薬剤抵抗性におけるMDP1の役割

    仁木 誠, 吉村 満美子, 和田 崇之, 小林 和夫, 松本 壮吉

    日本細菌学雑誌   62 ( 1 )   151 - 151   2007.02（ ISSN:0021-4930 ）

  • 結核菌の細胞壁合成におけるmycobacterial DNA-binding protein 1(MDP1)の役割

    松本 壮吉, 奥山 めぐみ, 尾関 百合子, 内藤 真理子, 西内 由紀子, 藤原 永年, 吉村 満美子, 和田 崇之, 小林 和夫

    日本細菌学雑誌   62 ( 1 )   91 - 91   2007.02（ ISSN:0021-4930 ）

  • 抗酸菌の肺胞上皮細胞侵入におけるMycobacterial DNA-binding protein1(MDP1)とヒアルロン酸の役割

    平山 幸雄, 松本 壮吉, 小林 和夫, 和田 崇之, 尾関 百合子, 西内 由起子, 山本 三郎

    結核   80 ( 3 )   268 - 268   2005.03（ ISSN:0022-9776 ）

  • 結核菌の肺胞上皮細胞への接着/侵入における,ヒアルロン酸-MDP1結合の役割

    平山 幸雄, 松本 壮吉, 和田 崇之, 尾関 百合子, 梅森 清子, 山本 三郎, 西内 由紀子, 松本 真, 小林 和夫

    日本細菌学雑誌   60 ( 1 )   104 - 104   2005.02（ ISSN:0021-4930 ）

  • 抗酸菌の肺胞上皮細胞接着における分子機構

    平山 幸雄, 松本 壮吉, 青木 圭子, 和田 崇之, 尾関 百合子, 松本 真, 小林 和夫

    日本細菌学雑誌   59 ( 1 )   184 - 184   2004.02（ ISSN:0021-4930 ）

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Presentations

  • A survey on social interaction via proximity detection Domestic conference

    2023.12 

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    Presentation type：Oral presentation (general)  

  • 尿路病原性大腸菌におけるシャペロン・アッシャー型線毛の網羅的探索 Domestic conference

    井上陽晴, 和田崇之

    第76回日本細菌学会関西支部総会  2023.11 

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    Presentation type：Oral presentation (general)  

  • 結核菌ゲノム解析を用いたあいりん地域における結核感染伝播の分析 Domestic conference

    山本香織, 田丸亜貴, 高橋峰子, 小向潤, 和田崇之

    第82回日本公衆衛生学会総会  2023.11 

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    Presentation type：Oral presentation (general)  

  • 飼育下魚類における非結核性抗酸菌症の発生状況について Domestic conference

    小野文菜, 吉田志緒美, 近藤広孝, 和田崇之, 山崎伸二

    第29回日本野生動物医学会大会  2023.09 

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    Presentation type：Oral presentation (general)  

  • 腸管毒素原性大腸菌が保有する病原性プラスミドの安定に関わる遺伝的要因の検討 Domestic conference

    瀨戸伶弥, 西川禎一, 和田崇之

    第44回日本食品微生物学会学術総会  2023.09 

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    Presentation type：Oral presentation (general)  

  • 伝播経路解明から疫学研究への転換を目指す結核菌ゲノミクス Invited Domestic conference

    和田崇之

    第98回日本結核・非結核性抗酸菌症学会学術講演会  2023.06 

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    Presentation type：Oral presentation (general)  

  • 大阪市における結核分子疫学を用いた外国生まれ結核患者の特徴の分析 Domestic conference

    山本香織, 津田侑子, 淺野瑞穂, 橋本美穂, 下内昭, 和田崇之

    第98回日本結核・非結核性抗酸菌症学会学術講演会  2023.06 

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    Presentation type：Oral presentation (general)  

  • 高齢者における結核の感染伝播の検討 Domestic conference

    山本香織, 竹内昌平, 蒲田脩圭里, 永石真知子, 小向潤, 吉田英樹, 松本健二, 和田崇之

    第80回日本公衆衛生学会  2021.12 

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    Presentation type：Oral presentation (general)  

  • astA保有大腸菌におけるF4相同性接着因子の検出 Domestic conference

    鈴木優希, 谷本佳彦, 陳彥霖, 西川禎一, 和田崇之

    第42回日本食品微生物学会  2021.09 

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    Presentation type：Oral presentation (general)  

  • 定型接着因子不明な分散接着性大腸菌の全ゲノム解析 Domestic conference

    山﨑康祐, 谷本佳彦, 陳彥霖, 西川禎一, 中台(鹿毛)枝里子, 和田崇之

    第42回日本食品微生物学会  2021.09 

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    Presentation type：Oral presentation (general)  

  • エキスパートセミナー：抗酸菌の遺伝子解析と分子疫学 Invited International conference

    和田崇之

    第94回日本結核病学会  2019.06 

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    Presentation type：Oral presentation (general)  

  • ゲノム科学の進展がもたらす結核対策への恩恵とわが国の状況 Invited International conference

    和田崇之

    第94回日本結核病学会  2019.06 

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    Presentation type：Oral presentation (general)  

  • 抗酸菌属における病原性とその動物症例 Invited International conference

    和田崇之, 吉田志緒美, 柳井徳磨

    環境微生物系学会合同大会2017  2017.08 

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    Presentation type：Oral presentation (general)  

    Venue：仙台  

  • ゲノム解析が解き明かす結核の拡散経緯とこれから Invited International conference

    和田崇之

    第20回国際結核セミナー  2015.03 

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    Presentation type：Oral presentation (general)  

    Venue：東京  

  • 結核分子疫学研究の現在と未来 Invited International conference

    和田崇之

    第19回国際結核セミナー  2014.03 

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    Presentation type：Oral presentation (general)  

    Venue：東京  

  • 反復配列多型（VNTR）分析法による結核菌型別―精度管理と考え方、使い方― Invited International conference

    和田崇之

    第72回日本公衆衛生学会総会  2013.10 

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    Presentation type：Oral presentation (general)  

    Venue：津  

  • シンポジウムIV. 分子疫学研究の進歩と対策への応用. 結核菌の反復配列多型 (VNTR) 解析におけるローカライジングと国際標準化 Invited International conference

    和田崇之

    第83回日本結核病学会総会  2008.04 

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    Presentation type：Oral presentation (general)  

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Industrial Property Rights

  • 結核菌の型別法

    前田伸司, 和田崇之, 加藤誠也

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    property_type：Patent 

    Application no：特願2010-093140 

    Announcement no：特開2011-217703 

Grant-in-Aid for Scientific Research

  • Molecular mechanisms and principles of horizontal gene transfer occurring in coexistence of Mycobacteria

    Grant-in-Aid for Scientific Research(B)  2026

  • Molecular mechanisms and principles of horizontal gene transfer occurring in coexistence of Mycobacteria

    Grant-in-Aid for Scientific Research(B)  2025

  • Establishment of fimbriomics to insight into host adaptation risk of infectious bacteria

    Grant-in-Aid for Challenging Research (Exploratory)  2024

  • Molecular mechanisms and principles of horizontal gene transfer occurring in coexistence of Mycobacteria

    Grant-in-Aid for Scientific Research(B)  2024

  • An Interdisciplinary Study on the Reconstruction of the Asian Fermented Food Cultural Region

    Grant-in-Aid for Scientific Research(A)  2024

  • 分散接着性大腸菌による腸炎抑制メカニズム解明とプロバイオティクスへの応用

    Grant-in-Aid for Scientific Research(B)  2024

  • 感染性細菌の宿主適応リスクを見据えるフィンブリオミクスの創出

    Grant-in-Aid for Challenging Research (Pioneering)/(Exploratory)  2023

  • 分散接着性大腸菌による腸炎抑制メカニズム解明とプロバイオティクスへの応用

    Grant-in-Aid for Scientific Research(B)  2023

  • Testing dilution versus amplification effects of primate biodiversity on parasite biodiversity

    Grant-in-Aid for Scientific Research(B)  2023

  • 社会的ジレンマが生み出す薬剤耐性菌の蔓延防止戦略

    Grant-in-Aid for Scientific Research(B)  2023

  • アジア発酵食文化圏の再構築を目指す学際的研究

    Grant-in-Aid for Scientific Research(A)  2023

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Incentive donations / subsidies

  • 全国の醤油醸造蔵から分離される耐塩性乳酸菌のゲノム多様性と菌株保全

    公益財団法人 発酵研究所  2023

  • 野生動物から分離される大腸菌の腸管付着因子はいかにして宿主適応しているのか

    公益財団法人かなえ医薬振興財団  2017

  • 多様な時系列標本から分離されるヒト結核菌のゲノム情報抽出と分子進化学的検討

    公益財団法人 発酵研究所  2010

  • ゲノム比較および疫学的情報に基づいた結核菌の統合的理解と分子型別監視体制の確立

    公益財団法人黒住医学研究振興財団  2008

  • 結核菌の新規分子疫学的手法MIRU-VNTR法の評価および応用

    大同生命厚生事業団  2007

Other subsidies, etc.

  • 菌株ゲノミクスと人流ビッグデータを繋ぐ新しい感染症分子疫学の導出

    大阪公立大学  2020.04

  • 腸内細菌が宿主適応する仕組みを追う:定着因子と宿主決定をつなぐ分子進化ダイナミクス

    長崎大学  2019.06

  • ルワンダ共和国における小児医療の現況調査および小児保健のためのグローバル疫学連携

    長崎大学  2018.04

  • 第2回抗酸菌研究会開催支援

    公益財団法人大山健康財団  2017.04

Charge of on-campus class subject

  • 食品衛生学実験

    2024   Weekly class   Undergraduate

  • 微生物学

    2024   Weekly class   Undergraduate

  • 微生物学

    2024   Weekly class   Undergraduate

  • 社会・環境と健康1

    2024   Weekly class   Undergraduate

  • 食栄養学概論

    2024   Weekly class   Undergraduate

  • 食栄養学研究基礎演習1

    2024   Intensive lecture   Graduate school

  • 微生物学特論演習A

    2024   Intensive lecture   Graduate school

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Social Activities　⇒ Link to the list of Social Activities

  • 結核分子疫学調査の最新情報

    Role(s)： Lecturer

    Type： Research consultation

    第１回県央・県南地域結核コホート検討会  2018.08

  • 結核菌VNTR型別に関する実習講義

    Role(s)： Lecturer

    Type： Research consultation

    平成26年度 結核菌VNTR技術研修会（地域保健総合推進事業）  2014.11

  • 結核菌の遺伝的多様性がもたらす研究展開：公衆衛生から自然史まで

    Role(s)： Lecturer

    長崎県職臨床技師会研修会  2013.08

  • 結核分子疫学の用途と展望

    Role(s)： Lecturer

    長崎県南保健所  2013.06

  • 我が国における結核分子疫学の用途と展望

    Role(s)： Lecturer,　Official expert

    大分県衛生環境研究センター  2013.04

  • 罹患率に即した結核分子疫学の用途と展望

    Role(s)： Lecturer

    大阪市結核対策評価委員会  2013.01

  • 地域内の結核菌を効率よく型別するためのVNTR分析法

    Role(s)： Lecturer

    H24年度地域保健総合推進事業（岡山）  2012.12

  • ＶＮＴＲ型別解析の結核分子疫学への応用

    Role(s)： Lecturer

    京都市感染症診査協議会（結核部会）研究会  2012.03

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