Updated on 2023/04/04

写真a

 
Sumitani Jun-ichi
 
Organization
Graduate School of Agriculture Department of Applied Biological Chemistry Associate Professor
School of Agriculture Department of Applied Biological Chemistry
Title
Associate Professor
Affiliation
Institute of Agriculture
Contact information
メールアドレス

Position

  • Graduate School of Agriculture Department of Applied Biological Chemistry 

    Associate Professor  2022.04 - Now

  • School of Agriculture Department of Applied Biological Chemistry 

    Associate Professor  2022.04 - Now

Degree

  • 博士(農学) ( Others )

Research Areas

  • Life Science / Applied biochemistry

Research Interests

  • 臨床診断薬

  • デンプン結合領域

  • アミラーゼ阻害剤

  • アミラーゼ

Research subject summary

  • (-)インドラクタムV(ILV)生合成に関与する遺伝子群の同定とその利用

  • 1-デオキシノジリマイシン(DNJ)生合成遺伝子群を用いたDNJ誘導体の効率的合成

  • レアメタル還元細菌の還元機構の解析とその利用

  • 糖質加水分解酵素の糖転移活性を利用した配糖体の合成

  • ホスホリラーゼ系酵素を用いた新規オリゴ糖の合成

  • マルチ触媒ドメインを有するアミラーゼの構造と機能

  • 放線菌二次代謝産物生合成に関する研究

  • Aspergillus aculeatusを用いた異種遺伝子大量発現系の構築

  • セルラーゼを用いた木質系バイオマスの有効利用

  • 分子進化工学的手法を用いた診断薬用酵素の開発

  • 放線菌由来蛋白性アミラーゼ阻害剤と標的アミラーゼとの相互作用に関する研究

  • CBM25に分類されるデンプン結合領域の構造機能相関と応用

Research Career

  • Studies on molecular interaction between proteinaceous alpha-amylase inhibitors and theirtargets

  • Structural and functional analysis of CBM25

  • Studies on cellulase system in Aspergillus aculeatus

    biomass 

  • Microbial reduction and recoverry of minor metal using metal ion-reducing bacteria.

  • Enzymatic synthesis of maltotriosyl glucosides using maltotriose-forming exo type α- amylase

  • Enzymatic synthesis of noble oligosaccharides using beta-glucan phosphorylases

  • Studies on amylases having malticatalytic domains from microbe

  • Studies on biosynthesis systems for secondary metabolites from Actinomycetes

    glucosidase inhibitors 

  • Enzymatic synthesis of noble oligosaccharides

  • Functional improvement of diagnostic enzymes

Professional Memberships

  • 日本農芸化学会

  • 日本生物工学会

  • 日本生化学会

Job Career (off-campus)

  • Toyo Jozo Co, Ltd., Researcher

    1991

  • 東洋醸造(株) 研究員

    1991

Papers

  • A component of the septation initiation network complex, AaSepM, is involved in multiple cellulose-responsive signaling pathways in Aspergillus aculeatus. Reviewed

    Tsumura R, Sawada K, Kunitake E, Sumitani JI, Kawaguchi T, Tani S.

    Appl. Microbiol. Biotechnol. 雑誌   Vol.105 ( No.4 )   1535 - 1546   2021.02

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    Kind of work:Joint Work  

  • Biogenic manganese oxides combined with 1-hydroxybenzotriazol and an Mn(II)-oxidizing enzyme from Pleosporales sp. Mn1 oxidize 3,4-dimethoxytoluene to yield 3,4-dimethoxybenzaldehyde. Reviewed

    Yoshimura Y, Tani S, Fujiwara M, Nakamura M, Sumitani JI, Kawaguchi T.

    J. Biosci. Bioeng. 雑誌   2021.01

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    Kind of work:Joint Work  

  • Chemical genetic approach using β-rubromycin reveals that a RIO kinase-like protein is involved in morphological development in Phytophthora infestans. Reviewed

    Tani S, Nishio N, Kai K, Hagiwara D, Ogata Y, Tojo M, Sumitani JI, Judelson HS, Kawaguchi T.

    Sci. Rep. 雑誌   Vol.10 ( No.1 )   2020.12

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    Kind of work:Joint Work  

  • Engineering of the Trichoderma reesei xylanase 3 promoter for efficient enzyme expression. Reviewed

    Hirasawa H, Shioya K, Furukawa T, Tani S, Sumitani J, Kawaguchi T, Morikawa Y, Ogasawara W

    Applied Microbiology and Biotechnology 雑誌   Vol.102 ( No.6 )   2737 - 2752   2018.03

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    Kind of work:Joint Work  

  • Dipeptidyl peptidase IV is involved in the cellulose-responsive induction of cellulose biomass-degrading enzyme genes in Aspergillus aculeatus Reviewed

    Shuji Tani, Shota Yuki, Emi Kunitake, Jun-ichi Sumitani & Takashi Kawaguchi

    Biosci Biotechnol Biochem 雑誌   81 ( 6 )   2017.06

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    Kind of work:Joint Work  

  • Site-saturation mutagenesis for β-glucosidase 1 from Aspergillus aculeatus to accelerate the saccharification of alkaline-pretreated bagasse Reviewed

    Baba Y, Sumitani J, Tanaka K, Tani S, Kawaguchi T

    Appl Microbiol Biotechnol 雑誌   100 ( 24 )   2016.12

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    Kind of work:Joint Work  

  • A high performance Trichoderma reesei strain that reveals the importance of Xylanase III in cellulosic biomass conversion Reviewed

    Nakazawa H, Kawai T, Ida N, Shida Y, Shioya K, Kobayashi Y, Okada H, Tani S, Sumitani J, Kawaguchi T, Morikawa Y, Ogasawara W

    Enzyme and Microbial Technology 雑誌   Vol.82   89 - 95   2016.01

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    Kind of work:Joint Work  

  • Characterization and gene cloning of a maltotriose-forming exo-amylase from Kitasatospora sp. MK-1785. Reviewed

    Kamon M, Sumitani J, Tani S, Kawaguchi T

    Applied Microbiology and Biotechnology 雑誌   99 ( 11 )   2015.06

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    Kind of work:Joint Work  

  • Effects of clbR overexpression on enzyme production in Aspergillus aculeatus vary depending on the cellulosic biomass-degrading enzyme species. Reviewed

    Kunitake E, Kawamura A, Tani S, Takenaka S, Ogasawara W, Sumitani J, Kawaguchi T

    Bioscience, Biotechnology, and Biochemistry 雑誌   79 ( 3 )   2015.03

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    Kind of work:Joint Work  

  • Construction of innovative cellulase-producing Trichoderma reesei strains for biomass saccharification.

    Morikawa Y, Ogasawara W, Kobayashi Y, Kawaguchi T, Okada H, Sumitani J, Tani S, Kawai T, Nakazawa H, Shioya K

    Lignocellulose Degradation and Biorefinary 著書 UNI PUBLISHERS CO., LTD   2015.03

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    Kind of work:Joint Work  

  • Diverse regulation of hydrolytic enzyme genes for cellulosic biomass degradation in filamentous fungi-with a central focus on Aspergillus

    Tani S, Sumitani J, and Kawaguchi T

    Lignocellulose Degradation and Biorefinary 著書 UNI PUBLISHERS CO., LTD   2015.03

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    Kind of work:Joint Work  

  • Biochemical and mutational analysis of beta-glucosidase 1 from Aspergillus aculeatus for molecular basis in cellobiose hydrolysis

    Baba Y, Sumitani J, Tani S, Kawaguchi T

    Lignocellulose Degradation and Biorefinary 著書 UNI PUBLISHERS CO., LTD   2015.03

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    Kind of work:Joint Work  

  • Characterization of Aspergillus aculeatus β-glucosidase 1 accelerating cellulose hydrolysis with Trichoderma cellulase system. Reviewed

    Baba Y, Sumitani J, Tani S, Kawaguchi T

    AMB Express 雑誌   5 ( 1 )   2015.01

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    Kind of work:Joint Work  

  • Crystal structures of glycoside hydrolase family 3 β-glucosidase 1 from Aspergillus aculeatus. Reviewed

    Suzuki K, Sumitani J, Nam YW, Nishimaki T, Tani S, Wakagi T, Kawaguchi T, Fushinobu S.

    Biochem J 雑誌   452 ( 2 )   211 - 221   2013.06

  • Reversible impairment of the ku80 gene by a recyclable marker in Aspergillus aculeatus. Reviewed

    Tani S, Tsuji A, Kunitake E, Sumitani J, Kawaguchi T

    AMB Express 雑誌   3 ( 1 )   2013.01

  • Analysis of the saccharification capability of high-functional cellulase JN11 for various pretreated biomasses through a comparison with commercially available counterparts. Reviewed

    Kawai T, Nakazawa H, Ida N, Okada H, Tani S, Sumitani J, Kawaguchi T, Ogasawara W, Morikawa Y, Kobayashi Y

    J Ind Microbiol Biotechnol 雑誌   39 ( 12 )   1741 - 1749   2012.12

  • A novel transcriptional regulator, ClbR, controls the cellobiose- and cellulose-responsive induction of cellulase and xylanase genes regulated by two distinct signaling pathways in Aspergillus aculeatus. Reviewed

    Kunitake E, Tani S, Sumitani J, Kawaguchi T

    Appl Microbiol Biotechnol 雑誌   2012.07

  • XlnR-independent signaling pathway regulates both cellulase and xylanase genes in response to cellobiose in Aspergillus aculeatus. Reviewed

    Tani, S., Kanamasa, S., Sumitani, J., Arai, M., Kawaguchi, T

    Curr Genet 雑誌   58 ( 2 )   93 - 104   2012.04

  • Construction of a recombinant Trichoderma reesei strain expressing Aspergillus aculeatus β-glucosidase 1 for efficient biomass conversion. Reviewed

    Nakazawa H, Kawai T, Ida N, Shida Y, Kobayashi Y, Okada H, Tani S, Sumitani JI, Kawaguchi T, Morikawa Y, Ogasawara W

    Biotechnol Bioeng 雑誌   109 ( 1 )   92 - 99   2012.01

  • Halophilic characterization of starch-binding domain from Kocuria varians α-amylase. Reviewed

    Yamaguchi R, Inoue Y, Tokunaga H, Ishibashi M, Arakawa T, Sumitani JI, Kawaguchi T, Tokunaga M.

    Int J Biol Macromol. 雑誌   50 ( 1 )   95 - 102   2012.01

  • Agrobacterium tumefaciens-mediated transformation of Aspergillus aculeatus for insertional mutagenesis. Reviewed

    Kunitake, E., Tani, S., Sumitani, J., Kawagucni, T.

    AMB Express 雑誌   1 ( 1 )   2011.12

  • Development of a Homologous Transformation System for Aspergillus aculeatus Based on the sC Gene Encoding ATP-Sulfurylase Reviewed

    H. ADACHI, S. TANI, S. KANAMASA, J. SUMITANI and T. KAWAGUCHI

    Biosci. Biotechnol. Biochem. 雑誌   73   1197 - 1199   2009.05

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    Kind of work:Joint Work  

  • Improvement of Aspergillus oryzae for hyperproduction of endoglucanase: expression cloning of cmc-1 gene of Aspergillus aculeatus Reviewed

    Rashid MH, Javed MR, Kawaguchi T, Sumitani J, Arai M

    Biotechnol. Lett. 雑誌   30   2165 - 2172   2008.12

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    Kind of work:Joint Work  

  • Development of an efficient production method for β-mannosidase by the creation of an overexpression system in Aspergillus aculeatus Reviewed

    Kanamasa S., Kawaguchi T., Takada G., Kajiwara S., Sumitani J., Arai M.

    Lett. Appl. Microbiol. 雑誌   45 ( 2 )   142 - 147   2007.08

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    Kind of work:Joint Work  

  • Cellobiohydrolase I (Cel7A) from Trichoderma reesei has chitosanase activity Reviewed

    Ike M., Ko Y., Yokoyama K., Sumitani J., Kawaguchi T., Ogasawara W., Okada H., Morikawa Y.

    J. Mol. Catal. B Enzymatic 雑誌   47 ( 41702 )   159 - 196   2007.07

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    Kind of work:Joint Work  

  • Alteration of substrate specificity of fructosyl amno acid oxidase from Fusarium oxysporum Reviewed

    Fujiwara M, Sumitani J, Koga S, Yoshioka I, Kouzuma T, Imamura S, Kawaguchi T,and Arai M

    Applied Microbiology and Biotechnology 雑誌   74 ( 4 )   813 - 819   2007.03

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    Kind of work:Joint Work  

  • Alteration of substrate specificity of fructosyl amno acid oxidase from Ulocladium sp.103 Reviewed

    Fujiwara M, Sumitani J, Koga S, Yoshioka I, Kouzuma T, Imamura S, Kawaguchi T,and Arai M

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING 雑誌   102 ( 3 )   241 - 243   2006.09

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    Kind of work:Joint Work  

  • Cloning and Sequencing of an Exoglucanase Gene from Streptomyces sp. M23, and Its Expression in Streptomyces lividans TK-24. Reviewed

    CHANG-SU PARK, TAKASHI KAWAGUCHI, JUN-ICHI SUMITANI ,

    Journal of Bioscience and Bioengineering 雑誌   99 ( 4 )   434 - 436   2005.04

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    Kind of work:Joint Work  

  • Transformation of Aspergillus aculeatus using the drug resistance gene of Aspergillus oryzae and the pyrG gene of Aspergillus nidulans. Reviewed

    S. Kanamasa(学生), K. Yamaoka(学生), T. Kawaguchi, J. Sumitani, M. Arai

    Biosci. Biotech. Biochem. 雑誌   Vol.67 ( No. 12 )   2661 - 2663   2003.12

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    Kind of work:Joint Work  

  • Overexpression of Aspergillus aculeatus cellobiohydrolase I in Aspergillus oryzae. Reviewed

    S. Kanamasa(学生), M. Mochizuki(学生), G. Takada(学生), T. Kawaguchi, J. Sumitani, M. Arai

    J. Biosci. Bioeng. 雑誌   Vol.95 ( No.6 )   627 - 629   2003.06

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    Kind of work:Joint Work  

  • Cloning and transcription analysis of the Aspergillus aculeatus No. F-50 endoglucanase 2 (cmc2) gene. Reviewed

    G. Takada(学生), M. Kawasaki(学生), M. Kitawaki(学生), T. Kawaguchi, J. Sumitani, K. Izumori, M. Arai

    J. Biosci. Bioeng. 雑誌   Vol.94 ( No.5 )   482 - 485   2002.11

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    Kind of work:Joint Work  

  • Purification and characterization of cellulase (CBH I and EGL 1) produced by thermophilic microorganism Streptomyces sp. M23. Reviewed

    C.-s. Park(学生), T. Kawaguchi, J. Sumitani, M. Arai

    Appl. Biol. Sci. 雑誌   Vol.7   27 - 35   2001.12

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    Kind of work:Joint Work  

  • Overexpression and purification of Aspergillus aculeatus b-mannosidase and analysis of the integrated gene in Aspergillus oryzae. Reviewed

    S. Kanamasa(学生), G. Takada, T. Kawaguchi, J. Sumitani, M. Arai

    J. Biosci. Bioeng. 雑誌   Vol.92 ( No.2 )   131 - 137   2001.12

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    Kind of work:Joint Work  

  • ベトナムにおける米を利用した伝統発酵食品 Reviewed

    長野宏子,安藤純子,粕谷志郎,レバンヌング,トウキムアイン,川口剛司,炭谷順一,荒井基夫,庄司善哉,飯渕貞明,大森正司,加藤みゆき,田中直義

    食生活研究 雑誌   Vol.20 ( No.1 )   23 - 30   2001.01

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    Kind of work:Joint Work  

  • Cloning and sequencing analysis of xylanase gene (xyn1) from alkali-tolerant filamentous fungus, Acremonium sp. TM-28. Reviewed

    T. Ardyati(学生), T. Kawaguchi, J. Sumitani, Y. Moriyama, M. Arai

    Appl. Biol. Sci. 雑誌   Vol.6   75 - 85   2000.12

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    Kind of work:Joint Work  

  • Isolation of a cellulolytic thermophilic microorganism and some properties of the produced cellulase. Reviewed

    C.-S. Park(学生), T. Kawaguchi, J. Sumitani, M. Arai

    Appl. Biol. Sci. 雑誌   Vol.6   101 - 109   2000.12

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    Kind of work:Joint Work  

  • New type of starch-binding domain: the direct repeat motif in the C-terminal region of Bacillus sp. no. 195 a-amylase contributes to starch binding and raw starch degrading Reviewed

    J. Sumitani, T. Tottori(学生), T. Kawaguchi, M. Arai

    Biochem. J. 雑誌   Vol.350   477 - 484   2000.09

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    Kind of work:Joint Work  

  • Cloning and secretive expression of the gene encoding the proteinaceous a-amylase inhibitor palm from Streptomyces corchorusii. Reviewed

    J. Sumitani, Y. Tsujimoto(学生), T. Kawaguchi, M. Arai

    J. Biosci. Bioeng. 雑誌   Vol.90 ( No.2 )   214 - 216   2000.08

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    Kind of work:Joint Work  

  • The conserved tryptophan-arginine-tyrosine motif of a proteinaceous a-amylase inhibitor T-76 from Streptomyces nitrosporeus is important for inhibition of animal a-amylases but not for an a-amylase from Bacillus sp no. 195. Reviewed

    J. Sumitani, N. Hattori(学生), Y. Nakamura(学生), Y. Okuda(学生), T. Kawaguchi, M. Arai

    J. Biosci. Bioeng. 雑誌   Vol.90 ( No.1 )   74 - 80   2000.01

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    Kind of work:Joint Work  

  • Cloning and sequencing of an endoglucanase gene from Scopulariopsis brevicaulis TOF-1212, and its expression in Saccharomyces cerevisiae. Reviewed

    F. Nakatani(学生), T. Kawaguchi, G. Takada, J. Sumitani, Y. Moriyama, M. Arai

    Biosci. Biotech. Biochem. 雑誌   Vol.64 ( No.6 )   1238 - 1246   2000.01

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    Kind of work:Joint Work  

  • Enzymatic hydrolysis of sugar rich okara, and utilization of the hydrolyzates for single cell protein and ethanol production. Reviewed

    K. Muzakhar(学生), T. Kawaguchi, J. Sumitani, S. Ogura, M. Arai

    Appl. Biol. Sci. 雑誌   Vol.5   13 - 29   1999.12

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    Kind of work:Joint Work  

  • Cloning and sequencing of b-mannosidase gene from Aspergillus aculeatus No. F-50. Reviewed

    G. Takada(学生), T. Kawaguchi, T. Kaga(学生), J. Sumitani, M. Arai

    Biosci. Biotech. Biochem. 雑誌   Vol.63 ( No.1 )   206 - 209   1999.01

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    Kind of work:Joint Work  

  • Expression of Aspergillus aculeatus No. F-50 cellobiohydrolase I (cbhI) and b-glucosidase 1 (bgl1) genes by Saccharomyces cerevisiae. Reviewed

    G. Takada(学生), T. Kawaguchi, J. Sumitai, M. Arai

    Biosci. Biotech. Biochem. 雑誌   Vol.62 ( No.8 )   1615 - 1618   1998.08

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    Kind of work:Joint Work  

  • Bacillus animal type a-amylase: Cloning and sequencing of the gene, and comparison of the deduced amino acid sequence with that of other amylases Reviewed

    J. Sumitani, H. Nagae(学生), T. Kawaguchi, M. Arai

    J. Ferment. Bioeng. 雑誌   Vol.85 ( No.4 )   428 - 432   1998.04

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    Kind of work:Joint Work  

  • Cloning, nucleotide sequence, and expression of the Clostridium thermocellum cellodextrin phosphorylase gene and its application to synthesis of cellulase inhibitors. Reviewed

    T. Kawaguchi, Y. Ikeuchi(学生), N. Tsutsumi(学生), A. Kan(学生), J. Sumitani, M. Arai

    J. Ferment. Bioeng. 雑誌   Vol.85 ( No.2 )   144 - 149   1998.02

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    Kind of work:Joint Work  

  • Cloning, nucleotide sequence, and transcriptional analysis of Aspergillus aculeatus No. F-50 cellobiohydrolase I (cbhI) gene. Reviewed

    G. Takada(学生), T. Kawaguchi, J. Sumitani, M. Arai

    J. Ferment. Bioeng. 雑誌   Vol.85 ( No.1 )   1 - 9   1998.01

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    Kind of work:Joint Work  

  • Cloning and sequencing of the cDNA encoding b-glucosidase 1 from Aspergillus aculeatus. Reviewed

    T. Kawaguchi, T. Enoki(学生), S. Tsurumaki(学生), J. Sumitani, M. Ueda, T. Ooi, M. Arai

    Gene 雑誌   Vol.173 ( No.2 )   287 - 288   1996.09

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    Kind of work:Joint Work  

  • Molecular-cloning and expression of proteinaceous a-amylase inhibitor gene from Streptomyces nirosporeus in Escherichia coli. Reviewed

    J. Sumitani(学生), T. Kawaguchi, N. Hattori(学生), S. Murao, M. Arai

    Biosci. Biotech. Biochem. 雑誌   Vol.57 ( No.8 )   1243 - 1248   1993.08

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    Kind of work:Joint Work  

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Books and Other Publications

  • Lignocellulose Degradation and Biorefinary

    Yutaro Baba, Jun-ichi Sumitani, Shuji Tani, Takashi Kawaguchi

    UNI PUBLISHERS CO., LTD  2015.03  ( ISBN:978-4-946450-36-5

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    Responsible for pages:191-198  

  • Lignocellulose Degradation and Biorefinary

    Yasushi Morikawa, Wataru Ogasawara, Takashi Kawaguchi, Jun-ichi Sumitani, Shuji Tani et al( Role: Joint author)

    UNI PUBLRSHERS CO., LTD  2015.03  ( ISBN:978-4-946450-36-5

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    Responsible for pages:166-176  

  • Lignocellulose Degradation and Biorefinary

    Shuji Tani, Jun-ichi Sumitani, Takashi Kawaguchi

    UNI PUBLISHERS CO., LTD  2015.03  ( ISBN:978-4-946450-36-5

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    Responsible for pages:177-182  

  • Development of a Homologous Transformation System for Aspergillus aculeatus Based on the sC Gene Encoding ATP-Sulfurylase

    H. ADACHI, S. TANI, S. KANAMASA, J. SUMITANI and T. KAWAGUCHI( Role: Joint author)

       2009.05 

  • Biotechnology of Lignocellulose Degradation and Biomass Utilization

    J. Sumitani, S. Tani, T. Konishi, A. Tao, M. Arai, and T. Kawaguchi( Role: Joint author)

    Ito Print Publishing Division  2008.12 

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    Responsible for pages:8  

  • Biotechnology of Lignocellulose Degradation and Biomass Utilization

    A. Tsuji, E. Kunitake, S. Tani, J. Sumitani, and T.Kawaguchi( Role: Joint author)

    Ito Print Publishing Division  2008.12 

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    Responsible for pages:7  

  • Biotechnology of Lignocellulose Degradation and Biomass Utilization

    E. Kunitake, S. Tani, J. Sumitani, and T. Kawaguchi( Role: Joint author)

    Ito Print Publishing Division  2008.12 

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    Responsible for pages:7  

  • Biotechnology of Lignocellulose Degradation and Biomass Utilization

    S. Tani, J. Sumitani, and T. Kawaguchi( Role: Joint author)

    Ito Print Publishing Division  2008.12 

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    Responsible for pages:5  

  • Biotechnology of Lignocellulose Degradation and Biomass Utilization

    Sumitani J., Tottori T., Sakamoto T., Kawaguchi T., Arai M.( Role: Joint author)

    UNI PUBLISHERS CO., LTD.  2003.12 

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    Responsible for pages:9  

  • Biotechnology of Lignocellulose Degradation and Biomass Utilization

    Kawaguchi T., Kanamasa S., Mochizuki M., Takada G., Sumitani J., Arai M.( Role: Joint author)

    UNI PUBLISHERS CO., LTD.  2003.12 

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    Responsible for pages:11  

  • Biotechnology of Lignocellulose Degradation and Biomass Utilization

    Rashid M.H., Kawaguchi T., Sumitani J., Arai M.( Role: Joint author)

    UNI PUBLISHERS CO., LTD.  2003.12 

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    Responsible for pages:8  

  • Biotechnology of Lignocellulose Degradation and Biomass Utilization

    Kanamasa S., Takada G., Kawaguchi T., Sumitani J., Kaga T., Arai M.( Role: Joint author)

    UNI PUBLISHERS CO., LTD.  2003.12 

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    Responsible for pages:8  

  • Biotechnology of Lignocellulose Degradation and Biomass Utilization

    Arai M., Kawaguchi T., Sumitani J.( Role: Joint author)

    UNI PUBLISHERS CO., LTD.  2003.12 

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    Responsible for pages:4  

  • Biotechnology of Lignocellulose Degradation and Biomass Utilization

    Park C.-s., Kawaguchi T., Sumitani J., Takada G., Izumori K., Arai M.( Role: Joint author)

    UNI PUBLISHERS CO., LTD.  2003.12 

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    Responsible for pages:10  

  • Genetics, Biochemistry and Ecology of Cellulose Degradation

    Ardyati T., Moriyama Y., Hirose T., Esaki T., Veerothai M., Sumitani J., Kawaguchi T., Arai M.( Role: Joint author)

    UNI PUBLISHERS CO., LTD.  1999.03 

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    Responsible for pages:7  

  • Biochemical Principles and Mechanisms of Biosynthesis and Biodegradation of Polymers

    Arai M., Kawaguchi T., Takada G., Sumitani J.( Role: Joint author)

    WILEY-VCH  1999.03 

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    Responsible for pages:10  

  • Genetics, Biochemistry and Ecology of Cellulose Degradation

    Takada G., Kawaguchi T., Yoneda T., Kawasaki M., Sumitani J., Arai M.( Role: Joint author)

    UNI PUBLISHERS CO., LTD.  1999.03 

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    Responsible for pages:10  

  • Genetics, Biochemistry and Ecology of Cellulose Degradation

    Kawaguchi T., Takada G., Kitawaki M., Komori K., Sumitani J., Arai M.( Role: Joint author)

    UNI PUBLISHERS CO., LTD.  1999.03 

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    Responsible for pages:9  

  • Genetics, Biochemistry and Ecology of Cellulose Degradation

    Sumitani J., Kawaguchi T., Sugimoto K., Kan A., Kimachi N., Ikeuchi Y., Tsutsumi N., Hayashi H., Arai M.( Role: Joint author)

    UNI PUBLISHERS CO., LTD.  1999.03 

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    Responsible for pages:10  

  • Genetics, Biochemistry and Ecology of Cellulose Degradation

    Nakatani F., Minami K., Moriyama Y., Mitsuiki S., Kawaguchi T., Sumitani J., Murao S., Arai M.( Role: Joint author)

    UNI PUBLISHERS CO., LTD.  1999.03 

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    Responsible for pages:10  

  • Genetics, Biochemistry and Ecology of Cellulose Degradation

    Muzakhar K., Hayashi H., Kawaguchi T., Sumitani J., Arai M.( Role: Joint author)

    UNI PUBLISHERS CO., LTD.  1999.03 

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    Responsible for pages:10  

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Presentations

Outline of collaborative research (seeds)

  • 酸化還元酵素を用いた生体成分測定系の開発

  • G3アミラーゼを用いたG3配糖体の酵素合成

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    エキソ型に非還元末端からマルトトリオース(G3)単位で切断する酵素(G3Amy)がG3単位で周囲の水酸基に転移する活性が高いことを利用して,アルコール性及びフェノール性水酸基を有する化合物にG3を転移したG3配糖体を効率よく酵素合成するためのシステムを開発する。

  • ホスホリラーゼ系酵素を用いた新規オリゴ糖の合成

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    近年,オリゴ糖は様々な生理活性を有することが明らかになり,機能性食品や機能性素材としての応用が期待されている.これらオリゴ糖は主に加水分解酵素の限定的加水分解反応や転移反応を利用して合成されている場合が多いが,反応効率と特異性の点で十分とは言えない状況である.ホスホリラーゼは,多糖やオリゴ糖の加リン酸分解とその逆反応を触媒する酵素の総称であるが,これらホスホリラーゼ系酵素の反応は合成の方向に平衡が傾いており,オリゴ糖合成の効率が高い.一般にホスホリラーゼ系酵素は基質認識が厳密であることが知られているが,中には寛容なものもあり,糖受容体となる単糖jまたはオリゴ糖を変換することでヘテロオリゴ糖の合成も可能となる。具体的にはセロオリゴ糖に作用するセロビオースホスホリラーゼ/セロデキストリンホスホリラーゼ,ラミナリオリゴ糖ホスホリラーゼなどについて検討を行っている。特にラミナリオリゴ糖ホスホリラーゼについては,希少な酵素であり,単一酵素にて安価なグルコースから高価なラミナリオリゴ糖が生成可能であり,その産業応用に期待が高まっている。

  • 蛋白性アミラーゼ阻害剤の肥満防止薬への利用

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    放線菌の生産する蛋白性アミラーゼ阻害剤は分子量約8,000の単純蛋白質で,大量発現系も確立されている.本阻害剤は100℃の加熱に対しても阻害活性を失わないことから,調理を伴う食品に添加しても阻害活性が残存し,アミラーゼ阻害によるダイエット効果が期待できる.本阻害剤遺伝子を各種植物体へ導入し,その塊茎または果実で発現させることで,害虫耐性を付与したり,ダイエット食材として利用される可能性が大いに期待される.

  • CBM25に分類されるデンプン結合領域の応用

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    我々が新たに同定したCBM25に分類されるデンプン結合領域(SBD)は既知のSBDよりもデンプンへの結合力が強いだけでなく,SephadexやSephacrylなどの既存のデキストラン系樹脂に強く結合する.この結合能を利用して,例えば組み換え蛋白精製のためのアフィニティタグとして利用が可能である.また,デンプン性基質だけでなく,キシランやβ-1,6グルカンにも結合するSBDも保持している.この結合特異性を解析することで目的の糖質に結合しうる変異SBDを設計出来る可能性も考えられる.

  • 自然界からの有用微生物の単離

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    我々の研究室では,創成時から有用微生物の単離を行ってきており,多大な成果を得ている.このノウハウを利用して,目的に適う微生物を自然界から迅速に単離し,その解析を行う.

  • 有用蛋白遺伝子の異種宿主における大量発現

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    我々は目的の遺伝子を発現させるために,様々な発現系(Escherichia coli, Bacillus subtilis, Streptomyces lividans, Saccharomyces cerevisiae, Pichia pastoris, Aspergillus oryzae, Aspergillus aculeatus)について検討を行っており,それぞれの系に置いて多くの知見を得ている.このノウハウを利用して目的遺伝子の大量発現を迅速に行う.

  • 分子進化工学的手法を用いた有用酵素の機能変換

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    部位特的変異またはランダム変異を導入することで,必要とする性質を付加した改良酵素の開発を行う.多くの酵素は立体構造が明らかでなく,構造機能相関について知見がないものが多いことから,酵素の改質には,ランダム変異が有用である.我々はこのランダム変異に関して,様々な知見を持っており,目的の酵素に適合した変異導入法(error-prone PCR,DNA shufflingなど)を用い,分子進化工学的手法によって目的の性質を獲得した変異酵素を得る.

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Charge of on-campus class subject

  • Practice in Food Hygiene

    2021   Practical Training  

  • Functional Molecular Biology

    2021    

  • Laboratory : Microbiology

    2021   Practical Training  

  • Topics in Applied Microbiology

    2021    

  • General Principles of Applied Biological Chemistry

    2021    

Visiting Lectures ⇒ Link to the list of Visiting Lectures

  • 微生物バイオテクノロジーが拓く循環型社会

    Category:Modern system science (knowledge information system, environmental system, educational welfare, psychology), Agricultural science (applied biology, biofunctional chemistry, green space environmental science)

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    Audience:Junior high school students, High school students, College students, Teachers, Researchers, General, Company, Civic organization

    目に見えない微生物はあまりなじみのない存在かもしれません。しかし土壌1グラム中には数億匹もの微生物が生息しています。講義では身近に存在する微生物の中から我々の生活や産業に役立つ微生物を探し出しその力を活用する研究について紹介します。

Academic Activities

  • Journal of Japan society for Energy and Resources

    Role(s): Peer review

    エネルギー・資源学会  2022.04 - 2023.03

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    Type:Academic society, research group, etc.