Updated on 2024/03/19

写真a

 
MAKINO Yasushi
 
Organization
Graduate School of Science Department of Chemistry Lecturer
School of Science Department of Chemistry
Title
Lecturer
Affiliation
Institute of Science
Contact information
メールアドレス
Affiliation campus
Nakamozu Campus

Position

  • Graduate School of Science Department of Chemistry 

    Lecturer  2022.04 - Now

  • School of Science Department of Chemistry 

    Lecturer  2022.04 - Now

Degree

  • 博士(理学) ( Others ) (   Osaka University )

Research Areas

  • Nanotechnology/Materials / Bio chemistry  / Biological Chemistry

  • Nanotechnology/Materials / Chemistry and chemical methodology of biomolecules  / Biomolecular Sciences

Research subject summary

  • 糖鎖の高感度構造解析

  • 酵素の高感度活性測定法の開発

  • 糖質関連酵素の精製と性質評価

Research Career

  • Purification and characterization of carbohydrate-metabolizing enzymes

    Glycogen phosphorylase, Glycogen debranching enzyme, Phosphorylase kinase  Joint Research in Japan

    2005.04 - Now 

  • Structural analysis of sugar chains

    Sugar chain, Pyridylamination, Fluorescent labeling  Joint Research in Japan

    2005.04 - Now 

  • Development of diagnostic agents for GSD III

    Glycogen storage disease type III (GSD III), Diagnostic agent  International Joint Research

    2005.04 - Now 

Professional Memberships

  • 日本農芸化学会

    2017.04 - Now   Domestic

  • The Japanese Society of Carbohydrate Research

    1998.04 - Now   Domestic

  • The Japanese Biochemical Society

    1994.04 - Now   Domestic

Papers

  • Essential Dextrin Structure as Donor Substrate for 4-α-Glucanotransferase in Glycogen Debranching Enzyme Reviewed

    Uno, R., Makino, Y.*, and Matsubara, H.

    The Journal of Biochemistry   2024.07

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

  • Intersubunit Communication in Glycogen Phosphorylase Influences Substrate Recognition at the Catalytic Sites Reviewed

    Kamada, N., Ikeda, A., Makino, Y.*, and Matsubara, H.

    Amino Acids   56   14   2024.02

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

  • Glycogen Debranching Pathway Deduced from Substrate Specificity of Glycogen Debranching Enzyme Reviewed

    Ikeda, A., Makino, Y.*, and Matsubara, H.

    Glycoconjugate Journal   39 ( 3 )   345 - 355   2022.06

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

  • New Approach to Prepare Fluorogenic Branched Dextrins for Assaying Glycogen Debranching Enzyme Reviewed

    Sakaguchi, M., Makino, Y.*, and Matsubara, H.

    Glycoconjugate Journal 雑誌 Springer   37   667 - 679   2020.12

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   Kind of work:Joint Work   International / domestic magazine:International journal  

  • A New Interpretation of Sulfate Activation of Rabbit Muscle Glycogen Phosphorylase Reviewed

    Fujii, Y., Makino, Y.*, and Sato, M.

    Glycoconjugate Journal 雑誌 Springer   35 ( 3 )   299 - 309   2018.06

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   Kind of work:Joint Work   International / domestic magazine:International journal  

  • 糖鎖MS/MSフラグメンテーションにおける2-ピリジルアミノ化(PA)の影響 Reviewed

    山垣 亮*, 牧野泰士

    Journal of the Mass Spectrometry Society of Japan 雑誌 日本質量分析学会   65 ( 6 )   297 - 300   2017.12

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    Kind of work:Joint Work  

  • Fragmentation of Oligosaccharides from Sodium Adduct Molecules Depends on the Position of N-Acetyl Hexosamine Residue in Their Sequences in Mass Spectrometry Reviewed

    Yamagaki, T.* and Makino, Y.

    Mass Spectrometry (Tokyo) 雑誌 The Mass Spectrometry Society of Japan   6 ( Spec Iss 2 )   2017.09

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    Kind of work:Joint Work  

  • Probing the Catalytic Site of Rabbit Muscle Glycogen Phosphorylase Using a Series of Specifically Modified Maltohexaose Derivatives Reviewed

    Nakamura, M., Makino, Y.*, Takagi, C., Yamagaki, T., and Sato, M.

    Glycoconjugate Journal 雑誌 Springer   34 ( 4 )   563 - 574   2017.08

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   Kind of work:Joint Work   International / domestic magazine:International journal  

  • Sensitive, Nonradioactive Assay of Phosphorylase Kinase through Measurement of Enhanced Phosphorylase Activity towards Fluorogenic Dextrin Reviewed

    Miyagawa, D., Makino, Y.*, and Sato, M.

    The Journal of Biochemistry 雑誌   159 ( 2 )   239 - 246   2016.02

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   Kind of work:Joint Work   International / domestic magazine:International journal  

  • Properties and Functions of the Storage Sites of Glycogen Phosphorylase Reviewed

    Makino, Y.*, Fujii, Y., and Taniguchi, M.

    The Journal of Biochemistry 雑誌   157 ( 6 )   451 - 458   2015.06

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    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)   Kind of work:Joint Work   International / domestic magazine:International journal  

  • Discrimination of Porcine Glycogen Debranching Enzyme Isozymes by the Ratios of Their 4-alpha-Glucanotransferase and Amylo-alpha-1,6-Glucosidase Activities Reviewed

    Doi, S., Makino, Y., and Omichi, K.

    The Journal of Biochemistry 雑誌 The Japanese Biochemical Society   147 ( 6 )   851 - 856   2010.06

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    Kind of work:Joint Work  

  • Sensitive Assay of Glycogen Phosphorylase Activity by Analyzing the Chain-Lengthening Action on a Fluorogenic Maltooligosaccharide Derivative Reviewed

    Makino, Y. and Omichi, K.

    The Journal of Biochemistry 雑誌 The Japanese Biochemical Society   146 ( 1 )   71 - 76   2009.07

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    Kind of work:Joint Work  

  • Inspection of the Activator Binding Site for 4-alpha-Glucanotransferase in Porcine Liver Glycogen Debranching Enzyme with Fluorogenic Dextrins Reviewed

    Yamamoto, E., Watanabe, Y., Makino, Y., and Omichi, K.

    The Journal of Biochemistry 雑誌 The Japanese Biochemical Society   145 ( 5 )   585 - 590   2009.05

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    Kind of work:Joint Work  

  • Donor Substrate Specificity of 4-alpha-Glucanotransferase of Porcine Liver Glycogen Debranching Enzyme and Complementary Action to Glycogen Phosphorylase on Debranching Reviewed

    Watanabe, Y., Makino, Y., and Omichi, K.

    The Journal of Biochemistry 雑誌 The Japanese Biochemical Society   143 ( 3 )   435 - 440   2008.03

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    Kind of work:Joint Work  

  • Active Site Mapping of Amylo-alpha-1,6-Glucosidase in Porcine Liver Glycogen Debranching Enzyme Using Fluorogenic 6-O-alpha-Glucosyl-Maltooligosaccharides Reviewed

    Yamamoto, E., Makino, Y., and Omichi, K

    The Journal of Biochemistry 雑誌 The Japanese Biochemical Society   141 ( 5 )   627 - 634   2007.05

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    Kind of work:Joint Work  

  • Activation of 4-alpha-Glucanotransferase Activity of Porcine Liver Glycogen Debranching Enzyme with Cyclodextrins Reviewed

    Watanabe, Y., Makino, Y., and Omichi, K.

    The Journal of Biochemistry 雑誌 The Japanese Biochemical Society   140 ( 1 )   135 - 140   2006.07

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    Kind of work:Joint Work  

  • Purification of Glycogen Debranching Enzyme from Porcine Brain: Evidence for Glycogen Catabolism in the Brain Reviewed

    Makino, Y., Omichi, K.

    Bioscience, Biotechnology, and Biochemistry 雑誌 Japan Society for Bioscience, Biotechnology, and Biochemistry   70 ( 4 )   907 - 915   2006.04

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    Kind of work:Joint Work  

  • Acceptor Specificity of 4-alpha-Glucanotransferases of Mammalian Glycogen Debranching Enzymes Reviewed

    Makino, Y., Omichi, K.

    The Journal of Biochemistry 雑誌 The Japanese Biochemical Society   139 ( 3 )   535 - 541   2006.03

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    Kind of work:Joint Work  

  • Fluorogenic Substrates of Glycogen Debranching Enzyme for Assaying Debranching Activity Reviewed

    Watanabe, Y., Makino, Y., Omichi, K.

    Analytical Biochemistry 雑誌 Elsevier   340 ( 2 )   279 - 286   2005.03

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    Kind of work:Joint Work  

  • Analysis of Fluorogenic Smith Degradation Products of 7-(1,3-Disulfonaphtyl)amino-Disaccharides for Linkage Position Analysis of Carbohydrates Reviewed

    Makino, Y., Yonezaki, K., Omichi, K.

    The Journal of Biochemistry 雑誌 The Japanese Biochemical Society   132 ( 6 )   961 - 966   2002.12

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    Kind of work:Joint Work  

  • Glycogen Debranching Enzyme in Bovine Brain Reviewed

    Narahara, E., Makino, Y., Omichi, K.

    The Journal of Biochemistry 雑誌 The Japanese Biochemical Society   130 ( 3 )   465 - 470   2001.09

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    Kind of work:Joint Work  

  • Structural Analysis of N-Linked Sugar Chains of Human Blood Clotting Factor IX Reviewed

    Makino, Y., Omichi, K., Kuraya, N., Ogawa, H., Nishimura, H., Iwanaga, S., Hase, S.

    The Journal of Biochemistry 雑誌 The Japanese Biochemical Society   128 ( 2 )   175 - 180   2000.08

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    Kind of work:Joint Work  

  • Processing Pathway Deduced from the Structures of N-Glycans in Carica papaya Reviewed

    Makino, Y., Shimazaki, A., Omichi, K., Odani, S., Hase, S.

    The Journal of Biochemistry 雑誌 The Japanese Biochemical Society   127 ( 6 )   1121 - 1126   2000.06

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    Kind of work:Joint Work  

  • A New Sugar Chain of the Proteinase Inhibitor from Latex of Carica papaya Reviewed

    Shimazaki, A., Makino, Y., Omichi, K., Odani, S., and Hase, S.

    The Journal of Biochemistry 雑誌 The Japanese Biochemical Society   125 ( 3 )   560 - 565   1999.03

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    Kind of work:Joint Work  

  • Analysis of Oligosaccharide Structures from the Reducing End Terminal by Combining Partial Acid Hydrolysis and a Two-Dimensional Sugar Map Reviewed

    Makino, Y., Omichi, K., and Hase, S.

    Analytical Biochemistry 雑誌 Academic Press   264 ( 2 )   172 - 179   1998.11

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    Kind of work:Joint Work  

  • Classification of Sugar Chains of Glycoproteins by Analyzing Reducing End Oligosaccharides Obtained by Partial Acid Hydrolysis Reviewed

    Makino. Y., Kuraya, N., Omichi, K., and Hase, S.

    Analytical Biochemistry 雑誌 Academic Press   238 ( 1 )   54 - 59   1996.06

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    Kind of work:Joint Work  

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Outline of collaborative research (seeds)

  • 糖鎖の構造解析

  • 高感度な酵素活性測定法の開発

  • 動植物からの酵素の精製

Charge of on-campus class subject

  • 化学特別演習2A

    2023   Intensive lecture   Graduate school

  • 化学特別演習1A

    2023   Intensive lecture   Graduate school

  • 化学特別研究2A

    2023   Intensive lecture   Graduate school

  • 化学特別研究1A

    2023   Intensive lecture   Graduate school

  • 研究企画ゼミナール

    2023   Intensive lecture   Graduate school

  • 基礎化学実験

    2023   Weekly class   Undergraduate

  • 分子科学実験II

    2023   Weekly class   Undergraduate

  • 物理化学I

    2023   Weekly class   Undergraduate

  • 有機生物化学

    2023   Weekly class   Undergraduate

  • 基礎化学実験

    2023   Weekly class   Undergraduate

  • 機能分子化学特論A

    2023   Weekly class   Graduate school

  • 化学特別演習2B

    2023   Intensive lecture   Graduate school

  • 化学特別演習1B

    2023   Intensive lecture   Graduate school

  • 化学特別研究2B

    2023   Intensive lecture   Graduate school

  • 化学特別研究1B

    2023   Intensive lecture   Graduate school

  • 分子科学課題実習

    2023   Practical Training   Undergraduate

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Number of papers published by graduate students

  • 2023

    Number of graduate students presentations:1

  • 2022

    Number of graduate students presentations:1

Social Activities

  • 堺高校プロフェッサーズセミナー 社会に活きる糖質化学

    2019.04 - 2020.03

  • 堺高校サマーサイエンスセミナー 化学発光による微量化合物の検出

    2018.04 - 2019.03

  • 堺高校サマーサイエンスセミナー 発色・発光による微量化合物の検出

    2014.04 - 2015.03