Updated on 2024/04/01

写真a

 
TANI Shuji
 
Organization
Graduate School of Agriculture Department of Applied Biological Chemistry Associate Professor
School of Agriculture Department of Applied Biological Chemistry
Title
Associate Professor
Affiliation
Institute of Agriculture
Homepage
https://www.omu.ac.jp/agri/shuji_e/

Position

  • Graduate School of Agriculture Department of Applied Biological Chemistry 

    Associate Professor  2022.04 - Now

  • School of Agriculture Department of Applied Biological Chemistry 

    Associate Professor  2022.04 - Now

Degree

  • 博士「農学」 （ Others ）

Research Areas

  • Life Science / Applied microbiology  / Applied Microbiology

Research subject summary

  • 植物病原卵菌の胞子嚢分化および植物感染機構に関する研究

  • 糸状菌セルラーゼ遺伝子群の発現制御機構に関する分子生物学的研究

Research Career

  • 植物病原卵菌の遊走子嚢分化と植物感染機構に関する研究

    ジャガイモ疫病菌、付着器形成、特異的阻害剤 

    2012.04 - Now 

  • 糸状菌における分子遺伝学・分子生物学

    糸状菌、遺伝子発現制御、環境応答、セルラーゼ 

    2005.04 - Now 

Professional Memberships

  • 日本植物病理学会

    2016.04 - Now   Domestic

  • セルラーゼ研究会

    2006.04 - Now   Domestic

  • 日本生物工学会

    2005.04 - Now   Domestic

  • 糸状菌分子生物学研究会

    2005.04 - Now   Domestic

  • 日本農芸化学会

    1997.04 - Now   Domestic

Committee Memberships (off-campus)

  • 令和4年度大阪府生徒研究発表会(第1部) ～大阪サイエンスデイ～ 審査委員   大阪府教育委員会  

    2023.10 

  • 令和3年度大阪府生徒研究発表会(第2部) ～大阪サイエンスデイ～ 審査委員   大阪府教育委員会  

    2021.12 

  • 運営委員   糸状菌分子生物学研究会  

    2020.10 - Now 

  • 参与   日本農芸化学会  

    2019.04 - Now 

  • 委員   セルラーゼ研究会  

    2018.04 - Now 

Papers

  • Cycloheximide in the nanomolar range inhibits seed germination of <i>Orobanche minor</i> Reviewed

    Nogami Ryosuke, Nagata Mari, Imada Risa, Kai Kenji, Kawaguchi Takashi, Tani Shuji

    Journal of Pesticide Science   advpub ( 0 )   2023.12（ ISSN:1348589X ）

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    Authorship：Last author,　Corresponding author   Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    <p>From the 992 samples of culture extracts of microorganisms isolated from soil in Japan, we found that the extract of <i>Streptomyces</i> sp. no. 226 inhibited <i>Orobanche minor</i> seed germination without significantly affecting the seed germination of <i>Trifolium pratense</i> and the growth of <i>Aspergillus oryzae</i> and <i>Escherichia coli</i>. Using ESI-MS, <sup>1</sup>H-NMR, and <sup>13</sup>C-NMR, we identified the active compound as cycloheximide. Cycloheximide had half-maximum inhibitory concentrations of 2.6 ng/mL for the inhibition of seed germination of <i>O. minor</i> and 2.5 µg/mL for that of the conidial germination of <i>A</i>. <i>oryzae</i>. Since cycloheximide is known to inhibit translation by interacting with ribosomal protein L28 (RPL28) in yeast, we investigated whether RPL protein of <i>O. minor</i> plays a critical role in the inhibition of <i>O. minor</i> seed germination. Our data suggested that <i>O. minor</i> RPL27A was not sensitive to cycloheximide by comparing it to the strain expressing <i>S. cerevisiae</i> RPL28. These findings suggest the presence of an unidentified mechanism by which cycloheximide hinders <i>O. minor</i> seed germination.</p>

    DOI： 10.1584/jpestics.d23-038

  • Independent, cooperative regulation of cellulolytic genes by paralogous transcription factors ClbR and ClbR2 in Aspergillus aculeatus. Reviewed

    Emi Kunitake, Takashi Kawaguchi, Shuji Tani

    Bioscience, biotechnology, and biochemistry   88 ( 2 )   212 - 219   2023.11（ ISSN:0916-8451 ）

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    The cellobiose-responsive regulator ClbR, a Zn(II)2Cys6 binuclear-cluster transcription factor, is a positive regulator of carbohydrate-active enzyme (CAZyme) genes responsive to cellulose in Aspergillus aculeatus. Because Zn(II)2Cys6 transcription factors tend to dimerize with proteins of the same family, we searched for a counterpart of ClbR and identified ClbR2, which is 42% identical to ClbR, as an interacting partner of ClbR by yeast two-hybrid screening. Genetic analyses suggested that ClbR and ClbR2 cooperatively regulate the expression of CAZyme genes in response to cellulose and 1,4-β-mannobiose in A. aculeatus. CAZyme genes under the control of the transcription factor ManR were regulated by ClbR and ClbR2, whereas those controlled by the transcription factor XlnR were regulated by ClbR, but not ClbR2. These findings suggest that ClbR participates in multiple regulatory pathways in A. aculeatus by altering an interacting factor.

    DOI： 10.1093/bbb/zbad156

    PubMed

  • C-terminus of serine–arginine protein kinase-like protein, SrpkF, is involved in conidiophore formation and hyphal growth under salt stress in Aspergillus aculeatus Reviewed

    Natsumi Kobayashi, Ryohei Katayama, Kentaro Minamoto, Takashi Kawaguchi, Shuji Tani*

    International Microbiology   2023.05

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    Authorship：Last author,　Corresponding author   Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    DOI： https://doi.org/10.1007/s10123-023-00373-x

  • A new function of a putative UDP-glucose 4-epimerase on the expression of glycoside hydrolase genes in Aspergillus aculeatus Reviewed

    Mizuki Kuga, Hidetoshi Shiroyanagi, Takashi Kawaguchi, Shuji Tani*

    Applied Microbiology and Biotechnology   107   785 - 795   2023.02

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    DOI： 10.1007/s00253-022-12337-8

  • Improvement of cellulosic biomass-degrading enzyme production by reducing extracellular protease production in Aspergillus aculeatus. Reviewed

    Yuko Yoshimura, Yuri Kobayashi, Takashi Kawaguchi, Shuji Tani*

    The Journal of general and applied microbiology   68 ( 3 )   143 - 150   2022.11（ ISSN:0022-1260 ）

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    We investigated the effects of deleting major extracellular protease-encoding genes on cellulolytic and xylanolytic enzyme production in Aspergillus aculeatus. We first investigated the effect of prtT deletion, a positive transcription factor for extracellular protease-encoding genes in Aspergillus, on extracellular protease production in A. aculeatus. Genetic analysis indicated that among the major extracellular proteases, pepIIa and pepIIb are controlled by PrtT, but pepI is not. Thus, we generated a mutant with deletion of the two genes prtT and pepI (ΔprtTΔpepI) and one with deletion of the three genes pepI, pepIIa, and pepIIb (ΔpepIΔIIaΔIIb). Extracellular protease activities decreased in both ΔprtTΔpepI and ΔpepIΔIIaΔIIb to 3% of that in the control strain (MR12). Comparative time-course analyses indicated that endoglucanase activity in ΔprtTΔpepI increased to double that in MR12. Xylanase activities increased in both ΔprtTΔpepI and ΔpepIΔIIaΔIIb to fourfold higher than that in MR12 at maximum. β-Glucosidase activities were increased in ΔprtTΔpepI and ΔpepIΔIIaΔIIb 1.3- and 1.4-fold higher than that in MR12 at maximum, respectively. Residual activities of endoglucanase, xylanase, and β-glucosidase after 7 days of incubation at 37°C in the culture supernatant were 63%, 36%, and 48% of the original in MR12. Residual endoglucanase activities were more than 80% of the original in ΔprtT, ΔprtTΔpepI, and ΔpepIΔIIaΔIIb. Residual xylanase activities were not improved in all test strains. β-Glucosidase remained almost 97% of the original in ΔprtTΔpepI. These findings indicated that the reduction of extracellular proteases effectively improved cellulolytic and xylanolytic enzyme production and stability in A. aculeatus.

    DOI： 10.2323/jgam.2021.10.005

    PubMed

  • Serine-arginine protein kinase-like protein, SrpkF, stimulates both cellobiose-responsive and D-xylose-responsive signaling pathways in Aspergillus aculeatus. Reviewed

    Ryohei Katayama, Natsumi Kobayashi, Takashi Kawaguchi, Shuji Tani*

    Current genetics   68 ( 1 )   143 - 152   2022.02（ ISSN:0172-8083 ）

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    Aspergillus aculeatus produces cellulolytic enzymes in the presence of their substrates. We screened a library of 12,000 A. aculeatus T-DNA-inserted mutants to identify a regulatory factor involved in the expression of their enzyme genes in response to inducers. We found one mutant that reduced the expression of FIII-avicelase (chbI) in response to cellulose. T-DNA was inserted into a putative protein kinase gene similar to AN10082 in A. nidulans, serine-arginine protein kinase F, SrpkF. Fold increases in srpkF gene expression in response to various carbon sources were 2.3 (D-xylose), 44 (Avicel®), 59 (Bacto™ Tryptone), and 98 (no carbon) compared with D-glucose. Deletion of srpkF in A. aculeatus resulted in a significant reduction in cellulose-responsive expression of chbI, hydrocellulase (cel7b), and FIb-xylanase (xynIb) genes at an early induction phase. Further, the srpkF-overexpressing strain showed upregulation of the srpkF gene from four- to nine-fold higher than in the control strain. srpkF overexpression upregulated cbhI and cel7b in response to cellobiose and the FI-carboxymethyl cellulase gene (cmc1) and xynIb in response to D-xylose. However, the srpkF deletion did not affect the expression of xynIb in response to D-xylose due to the less expression of srpkF under the D-xylose condition. Our data demonstrate that SrpkF is primarily involved in cellulose-responsive expression, though it has a potential to stimulate gene expression in response to both cellobiose and D-xylose in A. aculeatus.

    DOI： 10.1007/s00294-021-01207-x

    PubMed

  • Biogenic manganese oxides combined with 1-hydroxybenzotriazol and an Mn(II)-oxidizing enzyme from Pleosporales sp. Mn1 oxidize 3,4-dimethoxytoluene to yield 3,4-dimethoxybenzaldehyde Reviewed

    Yuko Yoshimura, Shuji Tani,* Maki Fujiwara, Makoto Nakamura, Jun-ichi Sumitani, Takashi Kawaguchi

    131 ( 5 )   475 - 482   2021.05

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  • A component of the septation initiation network complex, AaSepM, is involved in multiple cellulose-responsive signaling pathways in Aspergillus aculeatus. Reviewed

    Ryosuke Tsumura, Kazumi Sawada, Emi Kunitake, Jun-Ichi Sumitani, Takashi Kawaguchi, Shuji Tani

    Applied Microbiology and Biotechnology   105 ( 4 )   1535 - 1546   2021.01（ ISSN:0175-7598 ）

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    Various carbohydrate-active enzymes in Aspergillus are produced in response to physiological inducers, which is regulated at the transcriptional level. To elucidate the induction mechanisms in Aspergillus, we screened for new regulators involved in cellulose-responsive induction from approximately 10,000 Aspergillus aculeatus T-DNA-inserted mutants. We constructed the T-DNA-inserted mutant library using the host strain harboring the orotidine 5'-monophosphate decarboxylase gene (pyrG) under the control of the FIII-avicelase gene (cbhI) promoter. Thus, candidate mutants deficient in cellulose-responsive induction were positively screened via counter selection against 5-fluoroorotic acid (5-FOA). Among less than two hundred 5-FOA-resistant mutants, one mutant that the T-DNA inserted into the AasepM locus reduced the cbhI expression in response to cellulose. Since AaSepM is similar to Schizosaccharomyces pombe Cdc14p (E-value, 2e-20; identities, 33%), which is a component of the septation initiation network (SIN)-complex, we constructed an AasepM deletion mutant (ΔAasepM). We analyzed the expression of cellulase and xylanase genes in response to cellulose, septation, and conidiation in ΔAasepM. The AasepM deletion leads to delayed septation and decreased formation of the conidium chain in A. aculeatus but does not affect hyphal growth on minimal media. We also confirmed AaSepM's involvement in multiple cellulose-responsive signaling pathways of cellulase and xylanase genes under the control of the ManR-dependent, XlnR-dependent, and ManR- and XlnR-independent signaling pathways. KEY POINTS : • A new regulator for cellulolytic gene expression has been identified. • AaSepM is involved in septation and conidiation in A. aculeatus. • AasepM is involved in multiple cellulose-responsive signaling pathways.

    DOI： 10.1007/s00253-021-11110-7

    PubMed

  • Chemical genetic approach using β-rubromycin reveals that a RIO kinase-like protein is involved in morphological development in Phytophthora infestans Reviewed International coauthorship

    Scientific Reports 雑誌 Springer Nature   10 ( 1 )   2020.12

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  • Actinomycins inhibit the production of the siderophore pyoverdines in the plant pathogen Pseudomonas cichorii SPC9018. Reviewed

    Risa Maenaka, Shuji Tani, Yasufumi Hikichi, Kenji Kai*

    Bioscience, Biotechnology, and Biochemistry   84 ( 10 )   1975 - 1985   2020.10（ ISSN:0916-8451 ）

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    Pyoverdines, a group of peptide siderophores produced by Pseudomonas species, function not only in iron acquisition, but also in their virulence in hosts. Thus, chemical inhibition of pyoverdine production may be an effective strategy to control Pseudomonas virulence. In the plant pathogen Pseudomonas cichorii SPC9018 (SPC9018), pyoverdine production is required for virulence on eggplant. We screened microbial culture extracts in a pyoverdine-production inhibition assay of SPC9018 and found Streptomyces sp. RM-32 as a candidate-producer. We isolated two active compounds from RM-32 cultures, and elucidated their structures to be actinomycins X2 and D. Actinomycins X2 and D inhibited pyoverdine production by SPC9018 with IC50 values of 17.6 and 29.6 μM, respectively. Furthermore, pyoverdine production in other Pseudomonas bacteria, such as the mushroom pathogen P. tolaasii, was inhibited by the actinomycins. Therefore, these actinomycins may be useful as chemical tools to examine pyoverdine functions and as seed compounds for anti-Pseudomonas virulence agents.

    DOI： 10.1080/09168451.2020.1785839

    PubMed

  • A unique combination of two different quorum sensing systems in the β-rhizobium Cupriavidus taiwanensis. Reviewed

    Wakimoto T, Nakagishi S, Matsukawa N, Tani S, Kai K

    Journal of natural products   83 ( 6 )   1876 - 1884   2020.06（ ISSN:0163-3864 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    DOI： 10.1021/acs.jnatprod.0c00054

    PubMed

  • Engineering of the Trichoderma reesei xylanase3 promoter for efficient enzyme expression Reviewed

    Hirasawa H, Shioya K, Furukawa T, Tani S, Sumitani J, Kawaguchi T, Morikawa Y, Shida Y, Ogasawara W.

    Applied Microbiology and Biotechnology 雑誌 Springer   2018.02

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  • Dipeptidyl peptidase IV is involved in the cellulose-responsive induction of cellulose biomass-degrading enzyme genes in Aspergillus aculeatus Reviewed

    Shuji Tani,* Shota Yuki, Emi Kunitake, Jun-ichi Sumitani, Takashi Kawaguchi.

    Bioscience, Biotechnology, and Biochemisry 雑誌 Japan Society for Bioscience, Biothechnology, and Agrochemistry   81 ( 6 )   1227 - 1234   2017.03

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  • Site-saturation mutagenesis for beta-glucosidase 1 from Aspergillus aculeatus to accelerate the saccharification of alkaline-pretreated bagasse. Reviewed

    Yutaro Baba, Jun-ichi Sumitani,* Kiyotaka Tanaka, Shuji Tani, Takashi Kawaguchi

    Applied Microbiology and Biotechnology 雑誌 Springer   100 ( 24 )   10495 - 10057   2016.12

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  • A high performance Trichoderma reesei strain that reveals the importance of xylanase III in cellulosic biomass conversion. Reviewed

    Hikaru Nakazawa, Tetsushi Kawai, Noriko Ida, Yosuke Shida, Kouki Shioya, Yoshinori Kobayashi, Hirofumi Okada, Shuji Tani, Jun-ichi Sumitani, Takashi Kawaguchi, Yasushi Morikawa, and Wataru Ogasawara*

    Enzyme and Microbial Technology 雑誌 Elsevier   82   89 - 95   2016.01

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  • Cytotoxicity and colloidal behavior of polystyrene latex nanoparticles toward filamentous fungi in isotonic solutions Reviewed

    Toshiyuki Nomura,* Shuji Tani, Makoto Yamamoto, Takumi Nakagawa, Shunsuke Toyoda, Eri Fujisawa, Akiko Yasui, Yasuhiro Konishi

    Chemosphere 雑誌 Elsevier   149   84 - 90   2016.01

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  • Characterization and gene cloning of a maltotriose-forming exo-amylase from Kitasatospora sp. MK-1785 Reviewed

    Masahiro Kamon, Jun-ichi Sumitani, Shigenori Nishimura, Shuji Tani, Takashi Kawaguchi

    Applied Microbiology and Biotechnology 雑誌 Springer   99 ( 11 )   4743 - 4753   2015.06

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  • Effects of clbR overexpression on enzyme production in Aspergillus aculeatus vary depending on the cellulosic biomass degrading enzyme species Reviewed

    Emi Kunitake, Ayano Kawamura, Shuji Tani,* Shigeo Takenaka, Wataru Ogasawara, Jun-ichi Sumitani, Takashi Kawaguchi.

    Bioscience, Biotechnology, and Biochemisry 雑誌 Japan Society for Bioscience, Biothechnology, and Agrochemistry   79 ( 3 )   488 - 495   2015.03

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  • Palmitate induces insulin resistance in human HepG2 hepatocytes by enhancing ubiquitination and proteasomal degradation of key insulin signaling molecules Reviewed

    Megumi Ishii, Ayumi Maeda, Shuji Tani, Mitsugu Akagawa*

    Archives of Biochemistry and Biophysics 雑誌 Elsevier   566   26 - 35   2015.01

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  • Characterization of Aspergillus aculeatus beta-glucosidase 1 accelerating cellulose hydrolysis with Trichoderma cellulase system Reviewed

    Yutaro Baba, Jun-ichi Sumitani, Shuji Tani, Takashi Kawaguchi

    AMB Express 雑誌 Springer   5 ( 1 )   2015.01

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  • Complex regulation of hydrolytic enzyme genes for cellulosig biomass degradation in filamentous fungi Invited Reviewed

    Shuji Tani,* Takashi Kawaguchi, Tetsuo Kobayashi

    Applied Microbiology and Biotechnology 雑誌 Springer   98 ( 11 )   4829 - 4837   2014.06

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  • Cristal structures of glycoside hydrolase family 3 beta-glucosidase 1 from Aspergillus aculeatus. Reviewed

    Kentaro Suzuki, Jun-ichi Sumitani, Toru Nishimaki, Shuji Tani, Takayoshi Wakagi, Takashi Kawaguchi, Shinya Fushinobu*

    Biochemical Journal (Epub on 28 March, 2013) 雑誌 Portland Press   452 ( 2 )   211 - 221   2013.06

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  • A novel transcriptional regulator, ClbR, controls the cellobiose- and cellulose-responsive induction of cellulase and xylanase genes regulated by two distinct signaling pathways in Aspergillus aculeatus. Reviewed

    Emi Kunitake, Shuji Tani,* Jun-ichi Sumitani, Takashi Kawaguchi.

    Applied Microbiology and Biotechnology 雑誌 Springer   97 ( 5 )   2017 - 2028   2013.03

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  • Reversible impairment of the ku80 gene by a recyclable marker in Aspergillus aculeatus. Reviewed

    Shuji Tani, Atsushi Tsuji, Emi Kunitake, Jun-ichi Sumitani, and Takashi Kawaguchi*

    AMB Express 雑誌 Springer   3   2013.01

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  • Analysis of the saccharification capability of high-functional cellulase JN11 for various pretreated biomasses through a comparison with commercially available counterparts. Reviewed

    Tetsushi Kawai, Hikaru Nakazawa, Noriko Ida, Hirofumi Okada, Shuji Tani, Sumitani Jun-ichi, Takashi Kawaguchi, Wataru Ogasawara, Morikawa Yasushi, Yoshinori Kobayashi*

    Journal of Industrial Microbiology & Biotechnology 雑誌 Springer   39 ( 12 )   1741 - 1749   2012.12

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  • XlnR-independent signaling pathway regulates both cellulase and xylanase genes in response to cellobiose in Aspergillus aculeatus. Reviewed

    Shuji Tani, Shin Kanamasa, Jun-ichi Sumitani, Motoo Arai, Takashi Kawaguchi*

    Current Genetics 雑誌 Springer   58 ( 2 )   93 - 104   2012.04

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  • Construction of a recombinant Trichoderma reesei strain expressing Aspergillus aculeatus beta-glucosidase 1 for efficient biomass conversion. Reviewed

    Hikaru Nakazawa, Tetsushi Kawai, Noriko Ida, Yosuke Shida, Yoshinori Kobayashi, Hirofumi Okada, Shuji Tani, Jun-ichi Sumitani, Takashi Kawaguchi, Yasushi Morikawa, and Wataru Ogasawara*

    Biotechnology and Bioengineering 雑誌 WILEY   109 ( 1 )   92 - 99   2012.01

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  • Agrobacterium tumefaciens-mediated transformation of Aspergillus aculeatus for insertional mutagenesis. Reviewed

    Emi Kunitake, Shuji Tani,* Jun-ichi Sumitani, Takashi Kawaguchi.

    AMB Express 雑誌 Springer   1   2011.12

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  • Metabolic adaptation of Phytophthora infestans during growth on leaves, tubers, and artificial media Reviewed

    Howard S. Judelson,* Shuji Tani, Reena Narayan

    Molecular Plant Pathology 雑誌 Wiley-Blackwell   16 ( 6 )   843 - 855   2009.07

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  • Development of a homologous transformation system for Aspergillus acuelatus based on the sC gene encoding ATP-sulfurylase Reviewed

    Hiromi Adachi, Shuji Tani,* Shin Kanamasa, Jun-ichi Sumitani, Takashi Kawaguchi

    Bioscience, Biotechnology, and Biochemisry 雑誌 Japan Society for Bioscience, Biothechnology, and Agrochemistry   73 ( 5 )   1197 - 1199   2009.05

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  • Inducer-Dependent Nuclear Localization of a Zn(II)<Sub>2</Sub>Cys<Sub>6</Sub> Transcriptional Activator, AmyR, in Aspergillus nidulans Reviewed

    Tomohiro Makita, Yoko Katsuyama, Shuji Tani, Hayato Suzuki, Naoki Kato, Richard B. Todd, Michael J. Hynes, Norihiro Tsukagoshi, Masashi Kato, and Tetsuo Kobayashi*

    Bioscience, Biotechnology, and Biochemisry 雑誌 Japan Society for Bioscience, Biothechnology, and Agrochemistry   73 ( 2 )   391 - 399   2009.02

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  • Transgene-induced silencing of the zoosporogenesis-specific PiNIFC gene cluster of Phytophthora infestans involves chromatin alterations Reviewed

    Howard S. Judelson, and Shuji Tani

    Eukaryotic Cell 雑誌 American Society for Microbiology   6 ( 7 )   1210 - 1218   2007.06

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  • Performance of a tetracycline-responsive transactivator system for regulating transgenes in the oomycete Phytophthora infestans Reviewed

    Howard S. Judelson,* Reena Narayan, Audrey M. V. Ah Fong, Shuji Tani, Kyoung Su Kim

    Current Genetics 雑誌 Springer Verlag   51 ( 5 )   297 - 307   2007.05

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  • Activation of Zoosporogenesis-specific genes in Phytophthora infestans involves a 7-nucleotide promoter motif and cold-induced membrane rigidity. Reviewed

    Shuji Tani, and Howard Judelson

    Eukaryotic Cell 雑誌 American Society for Microbiology   5 ( 4 )   745 - 752   2006.04

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  • A cluster of NIF transcriptional regulators with divergent patterns of spore-specific expression in Phytophthora infestans. Reviewed

    Shuji Tani, Kyoung-Su Kim, and Howard Judelson

    Fungal Genetics and Biology 雑誌 Academic Press INC Elsevier Science   42 ( 1 )   42 - 50   2005.01

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  • Mode of AmyR binding to the CGGN8CGG sequence in the Aspergillus oryzae taaG2 promoter. Reviewed

    T. Itoh, S. Tani, T. Itho, N. Tsukagoshi, M. Kato, and T. Kobayashi

    Bioscience, Biotechnology, and Biochemisry 雑誌 Japan Society for Bioscience, Biothechnology, and Agrochemistry   68 ( 9 )   1906 - 1911   2004.09

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  • Multiple pathways regulate the induction of genes during zoosporogenesis in Phytophthora infestans. Reviewed

    S. Tani, E. Yatzkan, and H. Judelson

    Molecular Plant-Microbe Interaction 雑誌 American Phytopathological Society   17 ( 3 )   330 - 337   2004.03

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  • in vivo and in vitro analyses of the AmyR binding site of the Aspergillus nidulans agdA promoter, requirement of the CGG direct repeat for induction and high affinity binding of AmyR. Reviewed

    S. Tani, T. Itoh, M. Kato, T. Kobayashi,* and N. Tsukagoshi

    Bioscience, Biotechnology, and Biochemisry 雑誌 Japan Society for Bioscience, Biothechnology, and Agrochemistry   65 ( 7 )   1568 - 1574   2001.06

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    Authorship：Lead author   Kind of work：Joint Work   International / domestic magazine：International journal  

  • Characterization of the amyR gene encoding a transcriptional activaor for the amylase genes in Aspergillus nidulans. Reviewed

    S. Tani, Y. Katsuyama, T. Hayashi, H. Suzuki, M. Kato, K. Gomi, T. Kobayashi,* and N. Tsukagoshi

    Curernt Genetics 雑誌 Springer Verlag   39 ( 1 )   10 - 15   2001.02

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    Authorship：Lead author   Kind of work：Joint Work   International / domestic magazine：International journal  

  • A novel nuclear factor, SREB, binds to a cis-acting element, SRE, required for inducible expression of the Aspergillus oryzae Taka-amylase A gene in A. nidulans. Reviewed

    S. Tani, T. Kawaguchi, M. Kato, T. Kobayashi,* and N. Tsukagoshi

    Molecular and General Genetics 雑誌 Springer Verlag   263 ( 2 )   232 - 238   2000.03

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    Authorship：Lead author   Kind of work：Joint Work   International / domestic magazine：International journal  

  • Purification and characterization of &beta;-1.3-xylanase form a marine bacterium, Vibrio sp. XY-214. Reviewed

    T. Araki,* S. Tani, K. Maeda, S. Hashikawa, H. Nakagawa, and T. Morishita

    Bioscience, Biotechnology, and Biochemisry 雑誌 Japan Society for Bioscience, Biothechnology, and Agrochemistry   63 ( 11 )   2017 - 2019   1999.11

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    Kind of work：Joint Work   International / domestic magazine：International journal  

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MISC

  • 糸状菌における形態形成機構と酵素生産を協調的に制御する分子機構の解明とその応用 Invited International journal

    谷　修治

    北隆館・アグリバイオ   7 ( 8 )   79 - 83   2023.08

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    Authorship：Lead author,　Last author,　Corresponding author   Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)   International / domestic magazine：Domestic journal  

  • Construction of innovative cellulase-producing Trichoderma reesei strains for biomass saccharification International journal

    Yasuyuki Morikawa, Wataru Ogasawara, Yoshiyuki Kobayashi, Takashi Kawaguchi, Hirofumi Okada, Jun-ichi Sumitani, Shuji Tani, Tetsushi Kawai, Hikaru Nakazawa, Koki Shioya

    Biotechnology of lignocellulose degradation, biomass utilization, and biorefinery Mie Bioforum   166 - 176   2015.03

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  • Biochemical and mutational analysis of &beta;-glucosidase 1 from Aspergillus aculeatus for mlecular basis in cellogiose hydrolysis. International journal

    Yutaro Baba, Jun-ichi Sumitani, Shuji Tani, Takashi Kawaguchi

    Biotechnology of lignocellulose degradation, biomass utilization, and biorefinery Mie Bioforum   191 - 198   2015.03

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  • Diverse regulation of hydrolytic enzyme genes for cellulosic biomass degradation in filamentous fungi -with a central focus on Aspergillus. International journal

    Shuji Tani, Jun-ichi Sumitani, Takashi Kawaguchi

    Biotechnology of lignocellulose degradation, biomass utilization, and biorefinery Mie Bioforum   177 - 182   2015.03

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    Authorship：Lead author   Kind of work：Joint Work   International / domestic magazine：International journal  

  • 糸状菌セルラーゼ遺伝子群の転写活性化機構の解析 Invited International journal

    國武絵美，谷修治，炭谷順一，川口剛司

    Jstage Mycotoxin   2014.01

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    Authorship：Corresponding author   Kind of work：Joint Work   International / domestic magazine：Domestic journal  

  • 多様な因子が制御する糸状菌セルロース系バイオマス分解酵素遺伝子の発現誘導機構の解明 Invited International journal

    谷　修治

    日本農芸化学会 化学と生物   51 ( 4 )   200 - 202   2013.04

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    Authorship：Lead author,　Corresponding author   Kind of work：Single Work   International / domestic magazine：Domestic journal  

  • Physiological inducer and cis-element required for the cellobiohydrolase I gene induction via XlnR-independent pathway in Aspergillus aculeatus. International journal

    Shuji Tani, Jun-ichi Sumitani, Takashi Kawaguchi

    Biotechnology of lignocellulose degradation, biomass utilization, and biorefinery Mie Bioforum   467 - 470   2009.08

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    Authorship：Lead author   Kind of work：Joint Work   International / domestic magazine：International journal  

  • Cloning of the genes encoding xylosidase from Aspergillus aculeatus and characterization of their products. International journal

    Jun-ichi Sumitani, Shuji Tani, Tatsuya Konishi, Aiko Tao, Motoo Arai, Takashi Kawguchi

    Biotechnology of lignocellulose degradation, biomass utilization, and biorefinery Mie Bioforum   415 - 421   2009.08

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  • Development of highly efficient gene-targeting system by disrupting Aspergillus aculeatus Ku80. International journal

    Atsushi Tsuji, Shuji Tani, Jun-ichi Sumitani, Takashi Kawaguchi

    Biotechnology of lignocellulose degradation, biomass utilization, and biorefinery Mie Bioforum   549 - 554   2009.08

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    Authorship：Corresponding author   Kind of work：Joint Work   International / domestic magazine：International journal  

  • Development of Agrobacterium tumefaciens-mediated transformation system for random insertional mutagenesis in Aspergillus aculeatus. International journal

    Emi Kunitake, Shuji Tani,* Jun-ichi Sumitani, Takashi Kawaguchi.

    Biotechnology of lignocellulose degradation, biomass utilization, and biorefinery Mie Bioforum   521 - 526   2009.08

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    Authorship：Corresponding author   Kind of work：Joint Work   International / domestic magazine：International journal  

  • Gene expresion and signal transduction during spore developtment in Phytophthora infestans International journal

    Judelson HS,* Fong AAh, Blanc F, Kim KS, and Tani S

    American Phytopathological Society Phytopathology   94 ( 6S )   S48 - S48   2004.06

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    Authorship：Last author   Kind of work：Joint Work   International / domestic magazine：International journal  

  • Mechanisms of temperature-dependent gene expression during zoosporogenesis in Phytophthora infestans International journal

    S. Tani and H. Judelson*

    American Phytopathological Society Phytopathology   94 ( 6S )   S101 - S101   2004.06

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    Authorship：Lead author   Kind of work：Joint Work   International / domestic magazine：International journal  

  • まず複合体ありき？TATA-box binding proteinのプロモーターへの結合 Invited International journal

    谷修治

    日本農芸化学会 化学と生物   38 ( 3 )   180 - 181   2000.03

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    Authorship：Lead author,　Last author,　Corresponding author   Kind of work：Single Work   International / domestic magazine：Domestic journal  

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Presentations

  • Aspergillus aculeatusにおいて形態形成制御因子はセロビオースに応答した遺伝子発現誘導を制御する Domestic conference

    志賀結衣，菊矢咲季，川口剛司，谷修治

    糸状菌分子生物学コンファレンス  2023.11 

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    Presentation type：Oral presentation (general)  

  • 卵菌 Phytophthora infestansの形態変化におけるアネキシン様タンパク質の機能解析 Domestic conference

    藤島里佐子，川口剛司，谷修治

    糸状菌分子生物学コンファレンス  2023.11 

  • 真菌における dipeptidyl peptidaseを介した酸化ストレス応答機構の解析 Domestic conference

    中谷早希，遊亀翔太，川口剛司，谷修治

    糸状菌分子生物学コンファレンス  2023.11 

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    Presentation type：Poster presentation  

  • Cycloheximideが1×10-8で根寄生植物 Orobanche minor の種子発芽を阻害する Domestic conference

    野上 凌介、永田 真梨、今田 理彩、甲斐 建次、川口 剛司、谷 修治

    日本農芸化学会関西支部第526回講演会  2023.07 

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    Presentation type：Oral presentation (general)  

  • UDP-glucose 4-epimerase participates in the selective gene expression in response to mannobiose triggered by ManR Domestic conference

    Mizuki KUGA, Jun-ichi SUMITANI, Takashi KAWAGUCHI, Shuji TANI

    the 2023 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry  2023.03  JSBBA

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    Presentation type：Oral presentation (general)  

    Venue：University of Hiroshima  

  • Functions of a septation initiation network component, SepL, in Aspergillus aculeatus Domestic conference

    Saki KIKUYA, Jun-ichi SUMITANI, Takashi KAWAGUCHI, Shuji TANI

    JSBBA2023  2023.03  JSBBA

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    Presentation type：Oral presentation (general)  

    Venue：Hiroshima  

  • 糸状菌の多糖分解酵素生産制御因子の機能解析と酵素大量生産系の構築 Invited Domestic conference

    谷 修治

    日本農芸化学会関西支部　第521回講演会  2022.07  日本農芸化学会

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    Presentation type：Symposium, workshop panel (nominated)  

    Venue：オンライン  

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Industrial Property Rights

  • 卵菌類防除剤

    谷修治，炭谷順一，川口剛

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    property_type：Patent 

  • セルラーゼ・ヘミセルラーゼ転写因子

    谷修治，炭谷順一，川口剛

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    property_type：Patent 

Outline of collaborative research (seeds)

  • 植物病原卵菌の疫病防除法の開発

  • バイオマスの糖化

  • 糸状菌におけるタンパク質発現

  • 糸状菌の遺伝子発現制御機構の解析

Grant-in-Aid for Scientific Research

  • 青枯病菌が示すユニークな真菌寄生機構の解明と化学制御

    Grant-in-Aid for Scientific Research(B)  2023

  • 糸状菌における環境応答と酵素生産を協調的に制御する分子機構の解明とその応用

    Grant-in-Aid for Scientific Research(C)  2023

  • 糸状菌における環境応答と酵素生産を協調的に制御する分子機構の解明とその応用

    Grant-in-Aid for Scientific Research(C)  2022

  • 青枯病菌が示すユニークな真菌寄生機構の解明と化学制御

    Grant-in-Aid for Scientific Research(B)  2022

Contract research

  • ストライガ防除による食料安全保障と貧困克服

    SATREPS  2022

  • ストライガ防除による食料安全保障と貧困克服

    JST, JICA  SATREPS  2021

  • ストライガ防除による食料安全保障と貧困克服

    -  SATREPS  2020

  • ストライガ防除による食料安全保障と貧困克服

    -  SATREPS  2019

  • ストライガ防除による食料安全保障と貧困克服

    -  SATREPS  2017

  • ストライガ防除による食料安全保障と貧困克服

    -  2016

  • セルロース誘導制御因子の機能を利用したセルラーゼ高生産糸状菌の創製

    国立研究開発法人科学技術振興機構  2015

  • (A-Step)セルロース誘導制御因子の機能を利用したセルラーゼ高生産糸状菌の創製

    独立行政法人科学技術振興機構  2014

  • セルロース誘導制御因子の機能を利用したセルラーゼ高生産糸状菌の創製

    国立研究開発法人科学技術振興機構  2014

  • セルロース系バイオマス分解酵素の大量生産に向けた糸状菌の分子育種

    独立行政法人科学技術振興機構  2011

  • セルロース系バイオマス分解酵素の大量生産に向けた糸状菌の分子育種

    国立研究開発法人科学技術振興機構  2011

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Incentive donations / subsidies

  • ブドウ果皮の着色不良を改善する微生物資源の探索

    一般社団法人ヤンマー資源循環支援機構  一般社団法人ヤンマー資源循環支援機構  2022

  • 卵菌の温度に応答した形態形成制御に関わる因子の同定と機能解析

    公益財団法人発酵研究所  2022

  • 植物病原卵菌Phytophthora infestansのシスト発芽阻害物質の作用機序解析

    公益財団法人発酵研究所  2016

Charge of on-campus class subject

  • 微生物機能開発学特論

    2023   Weekly class   Graduate school

  • 生命機能化学概論

    2023   Weekly class   Undergraduate

  • 微生物学実験

    2023   Weekly class   Undergraduate

  • 生物環境化学

    2023   Weekly class   Undergraduate

  • 生物代謝制御学

    2023   Weekly class   Undergraduate

  • 生命機能化学研究プレゼンテーション

    2023   Intensive lecture   Graduate school

  • 生命機能化学特論B

    2023   Weekly class   Graduate school

  • 微生物機能開発学特論

    2022   Weekly class   Graduate school

  • 生命機能化学概論

    2022   Weekly class   Undergraduate

  • 生命機能化学研究プレゼンテーション

    2022   Intensive lecture   Graduate school

  • 生命機能化学特論B

    2022   Weekly class   Graduate school

  • ゲノム解析の基礎

    2022   Practical Training   Undergraduate

  • 微生物学実験

    2022   Weekly class   Undergraduate

  • 生物環境化学

    2022   Weekly class   Undergraduate

  • 生物代謝制御学

    2022   Weekly class   Undergraduate

  • 基礎化学実験/必:農〈生命〉,選:農〈緑地〉

    2022   Weekly class   Graduate school

  • Laboratory : Microbiology

    2021   Practical Training  

  • Topics in Applied Microbiology

    2021    

  • General Principles of Applied Biological Chemistry

    2021    

  • Metabolic Regulation and Engineering

    2021    

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Number of papers published by graduate students

  • 2023

    Number of graduate students presentations：1

  • 2022

    Number of graduate students presentations：1

Number of instructed thesis, researches

  • 2023

    Number of instructed the graduation thesis：4 

    [Number of instructed the Master's Program] (previous term)：6 

    [Number of master's thesis reviews] (chief)：4  [Number of master's thesis reviews] (vice-chief)：4

  • 2022

    Number of instructed the graduation thesis：3 

    [Number of instructed the Master's Program] (previous term)：6  [Number of instructed the Master's Program] (letter term)：0

    [Number of master's thesis reviews] (chief)：2  [Number of master's thesis reviews] (vice-chief)：4

Media Coverage

  • 糸状菌の酵素遺伝子発現 Newspaper, magazine

    科学新聞社  科学新聞  2023.01

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    SDGs：