Updated on 2024/01/31

写真a

 
Hinenoya Atsushi
 
Organization
Graduate School of Veterinary Science Department of Veterinary Science Associate Professor
School of Veterinary Science Department of Veterinary Science
Title
Associate Professor
Affiliation
Institute of Veterinary Science

Position

  • Graduate School of Veterinary Science Department of Veterinary Science 

    Associate Professor  2022.04 - Now

  • School of Veterinary Science Department of Veterinary Science 

    Associate Professor  2022.04 - Now

Degree

  • Doctor (Veterinary Sciences) （ Others ） (   Osaka Prefecture University )

Research Areas

  • Life Science / Bacteriology  / Bacteriology

  • Life Science / Bacteriology

Research subject summary

  • Escherichia albertiiの病原性および分子疫学研究

  • 志賀毒素産生性大腸菌の病原性に関する研究

  • 大腸菌が産生する細胞膨化致死毒素の病原性に関する研究

Research Career

  • Approach to virulence mechanism of Escherichia albertii

    Individual

    2015.04 - Now 

  • Molecular epidemiology of Escherichia albertii

    International Joint Research

    2014.04 - Now 

  • Molecular epidemiology of Cytolethal distending toxin-producing Escherichia coli

    Cytolethal distending toxin, CDT, Escherichia coli, diarrhea  Individual

  • Analysis of pathogenecity of Shiga toxin-producing Escherichia coli

    Shiga toxin, STX, Escherichia coli, diarrhea  Individual

Professional Memberships

  • Japan Veterinary Medical Association

    2020.10 - Now

  • 日本食品微生物学会

    2015.01 - Now   Domestic

  • The Japanese Association for Infectious Diseases

    2011 - 2019   Domestic

  • AMERICAN SOCIETY FOR MICROBIOLOGY

  • THE JAPANESE ASSOCIATION FOR INFECTIOUS DISEASES

  • THE JAPANESE SOCIETY OF VETERINARY SCIENCE

  • JAPANESE SOCIETY FOR BACTERIOLOGY

  • JAPANESE SOCIETY OF FOOD MICROBIOLOGY

  • TOXIN SYMPOSIUM

  • 腸管出血性大腸菌感染症研究会

  • Enterohaemorrhagic Escherichia coli symposium

      Domestic

  • Japanese Society of Veterinary Science

      Domestic

  • Toxin Synposium

      Domestic

  • American Society for Microbiology

      Overseas

  • Japanese Society for Microbiology

      Domestic

▼display all

Awards

  • 黒屋奨学賞

    2021.03   Japanese Society for Bacteriology   Research on an emerging zoonotic pathogen Escherichia albertii

  • 研究奨励賞

    2016.11   腸管出血性大腸菌感染症研究会  

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    Country：Japan

  • 研究奨励賞

    2011.07   腸管出血性大腸菌感染症研究会  

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    Country：Japan

Job Career (off-campus)

  • Osaka Metropolitan University   Graduate School of Veterinary Science

    2022.04 - Now

  • University of Tennessee   College of Animal Science   Visiting Scholar

    2017.10 - 2019.02

  • Osaka Prefecture University   Department of Veterinary Science, Graduate School of Life and Environmental Sciences

    2017.04 - 2022.03

Papers

  • Cefixime-tellurite-deoxycholate tryptic soy broth (CTD-TSB), a selective enrichment medium, for enhancing isolation of Escherichia albertii from wild raccoon fecal samples.

    Bingting Xu, Noritoshi Hatanaka, Sharda Prasad Awasthi, Keiji Tekehira, Atsushi Hinenoya, Shinji Yamasaki

    Journal of applied microbiology   134 ( 7 )   2023.07（ ISSN:1364-5072 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    AIM: The aim of this study was to develop a selective enrichment broth for efficient isolation of Escherichia albertii. METHODS AND RESULTS: A total of 412 raccoon rectal swabs suspended in PBS (phosphate-buffered saline) were tested by a real-time PCR to quantify the number of E. albertii followed by its isolation. The number of E. albertii in the PBS suspension strongly affected the isolation rate (1.2%-89%), which notably dropped (≤33%) when the number was <4 log10 CFU ml-1. However, enrichment of PBS suspension containing raccoon feces in tryptic soy broth containing cefixime, tellurite, and deoxycholate (CTD-TSB), the selective medium developed in this study, remarkably improved the isolation efficiency (up to 48%) of E. albertii. CONCLUSIONS: CTD-TSB is a useful enrichment culture medium for E. albertii and contributes to increase its isolation rate.

    DOI： 10.1093/jambio/lxad123

    PubMed

  • Presence of Functionally Active Cytolethal Distending Toxin Genes on a Conjugative Plasmid in a Clinical Isolate of Providencia rustigianii.

    Hassan J, Awasthi SP, Hatanaka N, Hoang PH, Nagita A, Hinenoya A, Faruque SM, Yamasaki S

    Infection and immunity   e0012122   2023.05（ ISSN:0019-9567 ）

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  • Critical role of the RpoE stress response pathway in polymyxin resistance of Escherichia coli.

    Zeng X, Hinenoya A, Guan Z, Xu F, Lin J

    The Journal of antimicrobial chemotherapy   78 ( 3 )   732 - 746   2023.01（ ISSN:0305-7453 ）

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  • Endotoxin-Free Stx2B-C-CPE Vaccine and Its Optimized Adjuvant Regimen for Preventing Food Poisoning.

    Hosomi K, Shimoyama A, Hinenoya A, Hatanaka N, Noguchi T, Ebina H, Tojima Y, Furuta M, Kondoh M, Kiyono H, Yamasaki S, Fukase K, Kunisawa J

    Frontiers in bioscience (Landmark edition)   28 ( 1 )   15   2023.01（ ISSN:27686701 ）

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  • Seasonality of detection rate of <i>Escherichia albertii </i>in wild raccoons (<i>Procyon lotor</i>) in Osaka, Japan

    XU Bingting, HATANAKA Noritoshi, AWASTHI Sharda Prasad, HINENOYA Atsushi, YAMASAKI Shinji

    Journal of Veterinary Medical Science   advpub ( 0 )   2023（ ISSN:09167250 ） （ eISSN:13477439 ）

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    <p><i>Escherichia albertii </i>has increasingly been recognized as an important emerging zoonotic enteropathogen. Raccoon is shown to be one of the most vital reservoirs of this pathogen. <i>E. albertii </i>has been detected in 993 (62%) out of 1,606 wild raccoons in Osaka, Japan from 2017 to 2020 by <i>Eacdt</i>-PCR. The detection rate of <i>E. albertii </i>was increased from May to December (winter) and gradually decreased from January to April (spring). Furthermore, we could isolate <i>E. albertii</i> from 30% (196/664) of <i>Eacdt</i>-PCR positive samples and the monthly isolation rate seems to correlate with its detection rate. These data indicate that there is a seasonality regarding the prevalence of <i>E. albertii </i>in wild raccoon being higher in winter and lower in spring. </p>

    DOI： 10.1292/jvms.23-0372

    PubMed

  • 岡山県および県境に生息する野生動物および保護動物から分離したEscherichia albertiiの同定と性状解析(Isolation and characterization of Escherichia albertii from wild and safeguarded animals in Okayama Prefecture and its prefectural borders, Japan)

    Naka Atsushi, Hinenoya Atsushi, Awasthi Sharda Prasad, Yamasaki Shinji

    The Journal of Veterinary Medical Science   84 ( 9 )   1299 - 1306   2022.09（ ISSN:0916-7250 ）

  • Longitudinal surveillance and comparative characterization of Escherichia albertii in wild raccoons in the United States. Reviewed

    Atsushi Hinenoya, Huiwen Wang, Erin M Patrick, Ximin Zeng, Liu Cao, Xing-Ping Li, Rebecca L Lindsey, Barbara Gillespie, Qiang He, Shinji Yamasaki, Jun Lin

    Microbiological research   262   127109 - 127109   2022.07（ ISSN:0944-5013 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Escherichia albertii is an emerging enteric bacterial pathogen causing watery diarrhea, abdominal distension, vomiting and fever in humans. E. albertii has caused many foodborne outbreaks in Japan and was also reported in other countries worldwide. However, the important animal reservoirs of this pathogen are still largely unknown, impeding us to combat this emerging pathogen. Recently, we reported that wild raccoons (Procyon lotor) and broiler chickens are significant reservoirs of E. albertii in Japan and the U.S., respectively. Here, we performed a longitudinal surveillance to monitor prevalence of E. albertii in wild raccoons in the U.S. and conducted comprehensive comparative analyses of the E. albertii of different origins. A total of 289 fecal swab samples were collected from wild raccoons in Tennessee and Kentucky in the U.S. (2018-2020). Approximately 26% (74/289) of the raccoons examined were PCR-positive for E. albertii and eventually 22 E. albertii isolates were obtained. PFGE analysis showed the U.S. raccoon E. albertii were phylogenetically distant even though the corresponding raccoons were captured from a small area. Unlike the high prevalence of multidrug resistance (83%) observed in previous chicken E. albertii survey, antibiotic resistance was rarely observed in all the U.S. raccoon and 22 Japan raccoon strains with only one Japan strain displaying multidrug resistance (2%). Whole genome sequencing of 54 diverse E. albertii strains and subsequent comparative genomics analysis revealed unique clusters that displayed close evolutionary relationships and similar virulence gene profiles among the strains of different origins in terms of geographical locations (e.g., U.S. and Japan) and hosts (raccoon, chicken, swine, and human). Challenge experiment demonstrated raccoon E. albertii strains could successfully colonize in the chicken intestine at 3 and 8 days postinfection. A pilot environmental survey further showed all the four tested water samples from Tennessee river were E. albertii-positive; two different E. albertii strains, isolated from a single water sample, showed close relationships to those of human origin. Together, the findings from this study provide new insights into the ecology, evolution, and pathobiology of E. albertii, and underscore the need to control the emerging E. albertii in a complex ecosystem using One Health approach.

    DOI： 10.1016/j.micres.2022.127109

    PubMed

  • Corrigendum to "Prevalence of mobile colistin resistance (mcr) genes in extended-spectrum β-lactamase-producing Escherichia coli isolated from retail raw foods in Nha Trang, Vietnam" [Int. J. Food Microbiol. 346 (2021) 109164].

    Le PQ, Awasthi SP, Hatanaka N, Hinenoya A, Hassan J, Ombarak RA, Iguchi A, Tran NTT, Dao KVT, Vien MQ, Le HX, Do HT, Yamamoto Y, Yamasaki S

    International journal of food microbiology   370   109540   2022.06（ ISSN:0168-1605 ）

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  • Detection, Isolation, and Molecular Characterization of Escherichia albertii from Wild Birds in West Japan. Reviewed

    Atsushi Hinenoya, Sharda Prasad Awasthi, Noritomo Yasuda, Keigo Nagano, Jayedul Hassan, Keiji Takehira, Noritoshi Hatanaka, Shun Saito, Takashi Watabe, Miki Yoshizawa, Haruna Inoue, Shinji Yamasaki

    Japanese journal of infectious diseases   75 ( 2 )   156 - 163   2022.03（ ISSN:1344-6304 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：Domestic journal  

    Escherichia albertii is an emerging zoonotic foodborne pathogen. Several outbreaks of E. albertii have occurred, particularly in Japan. Although birds have been considered as one of the most important reservoirs of this bacterium, information regarding its prevalence in birds is still scarce. We performed a survey of E. albertii in wild birds in Japan and examined the characteristics of these isolates. E. albertii-specific genes were detected in five cloacal swabs from 156 birds by PCR. Four E. albertii strains were isolated from a swallow with two different E. albertii strains and two pigeons in a flock using XRM-MacConkey agar. These isolates were assigned to biogroup 3, showed no resistance to any tested antimicrobials, and were classified into two EAO-genotypes (EAOg2 and EAOg33) and were untypable. Similar to clinical E. albertii strains, these isolates carried virulence genes, including eae (n = 4), paa (n = 4), Eccdt-I (n = 2), and stx2f (n = 1), as well as Eacdt. Furthermore, stx2f genes in a strain were located on an inducible bacteriophage, which can confer the ability to produce Stx2f in E. coli. In conclusion, Japanese wild birds carried E. albertii at levels similar to the reported prevalence in birds. These isolates may have the potential to cause gastroenteritis in humans.

    DOI： 10.7883/yoken.JJID.2021.355

    PubMed

  • 西日本の野鳥からのEscherichia albertiiの検出、分離、分子的特性評価(Detection, Isolation, and Molecular Characterization of Escherichia albertii from Wild Birds in West Japan)

    Hinenoya Atsushi, Awasthi Sharda Prasad, Yasuda Noritomo, Nagano Keigo, Hassan Jayedul, Takehira Keiji, Hatanaka Noritoshi, Saito Shun, Watabe Takashi, Yoshizawa Miki, Inoue Haruna, Yamasaki Shinji

    Japanese Journal of Infectious Diseases   75 ( 2 )   156 - 163   2022.03（ ISSN:1344-6304 ）

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    大阪市と高知市の野鳥を対象にEscherichia albertii保有状況を調べた。ハトのクロアカスワブ検体129件と13種類の野鳥のクロアカスワブ検体27件を使用した。増菌培養後、Eacdt遺伝子を標的としたPCRでスクリーニングし、陽性検体をXRM-マッコンキー寒天培地またはマッコンキー寒天培地で培養した。その結果、ツバメ2羽(7.4%)、ハト3羽(2.3%)がPCR陽性となり、全体の検出率は3.2%(5/156)であった。培養検査ではツバメ1羽とハト2羽からE.albertiiが分離された。PFGE解析により、ハト由来株は同一クローン株、ツバメ由来株から2つのクローン株が確認された。分離4株は生物グループ3に割り当てられ、供試16種抗菌薬に感受性を示した。O抗原遺伝子型はツバメ由来2株が型別不能とEAOg2、ハト由来2株はEAOg33であった。分離4株はEacdtの他にeaeとpaaを保有していた。さらにツバメ由来1株はstx2f、ハト由来2株はEccdt-I遺伝子を保有していた。4株のインチミンサブタイプは多様であったが、LEE病原性アイランドの統合部位はphe Uのみであった。マイトマイシンC存在下、プラークアッセイによる溶原化ファージ誘発の結果、stx2fは誘発性ファージ上に位置し、ファージはレシピエント大腸菌にStx2f産生能を付与した。ドナー株のベロ毒素力価が増強された。

  • Piperine, an active ingredient of white pepper, suppresses the growth of multidrug‐resistant toxigenic Vibrio cholerae and other pathogenic bacteria Reviewed

    G.B. Manjunath, S.P. Awasthi, M.S.H. Zahid, N. Hatanaka, A. Hinenoya, E. Iwaoka, S. Aoki, T. Ramamurthy, S. Yamasaki

    Letters in Applied Microbiology   2022.02（ ISSN:0266-8254 ） （ eISSN:1472-765X ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/lam.13646

    Other URL： https://onlinelibrary.wiley.com/doi/full-xml/10.1111/lam.13646

  • Detection, isolation and molecular characterization of Escherichia albertii in wild birds in West Japan.

    Hinenoya A, Awasthi SP, Yasuda N, Nagano K, Hassan J, Takehira K, Hatanaka N, Saito S, Watabe T, Yoshizawa M, Inoue H, Yamasaki S

    Japanese Journal of Infectious Diseases   in press   2022

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    Kind of work：Joint Work  

  • Isolation and characterization of <i>Escherichia albertii</i> from wild and safeguarded animals in Okayama Prefecture and its prefectural borders, Japan Reviewed

    NAKA Atsushi, HINENOYA Atsushi, AWASTHI Sharda Prasad, YAMASAKI Shinji

    Journal of Veterinary Medical Science   84 ( 9 )   1299 - 1306   2022（ ISSN:09167250 ） （ eISSN:13477439 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：Domestic journal  

    <p><i>Escherichia albertii</i> has recently been recognized as a zoonotic enteropathogen associated with food poisoning. The reservoirs and transmission routes of this bacterium to humans are still unclear. In this study, we performed a survey of <i>E. albertii</i> in fecal specimens of wild and safeguarded animals in Okayama Prefecture and its prefectural borders, Japan to understand its reservoir in the environment. Forty-two <i>E. albertii</i> were isolated from 10 and 31 droppings of 59 crows and 125 starlings, respectively. Fifty-two <i>E. albertii</i> were isolated from 906 mammal droppings, and out of 52 isolates, origin of 33, 6 and 1 isolates were from martens, foxes, and rabbit, respectively, however, origin of 12 isolates remained unknown. Three <i>E. albertii</i> were isolated from two and one feces of 159 dogs and 76 cats, respectively. Pulsed-filed gel electrophoresis analysis grouped 97 <i>E. albertii</i> strains into 66 pulsotypes including 36 and 30 pulsotypes of isolates from mammals and birds, respectively. <i>E. albertii</i> strains isolated in this study were genetically diverse. Although clonal relationship was not observed between mammal and bird isolates, there were intra- and inter-species relationship in mammalian isolates. All <i>E. albertii</i> strains were positive for <i>eae</i> and <i>Eacdt</i> virulence genes. Furthermore, 20 and 7 strains also carried <i>Eccdt-I</i> and <i>stx2f</i> genes, respectively. Taken together, the results indicate that genetically diverse and potentially virulent <i>E. albertii</i> are distributed among various wild and safeguarded animals in Okayama Prefecture, and the animals could also be reservoirs of <i>E. albertii</i>.</p>

    DOI： 10.1292/jvms.22-0213

    PubMed

  • Identification of a Recently Dominant Sublineage in Salmonella 4,[5],12:i:- Sequence Type 34 Isolated From Food Animals in Japan Reviewed

    Nobuo Arai, Tsuyoshi Sekizuka, Yukino Tamamura-Andoh, Lisa Barco, Atsushi Hinenoya, Shinji Yamasaki, Taketoshi Iwata, Ayako Watanabe-Yanai, Makoto Kuroda, Masato Akiba, Masahiro Kusumoto

    Frontiers in Microbiology   12   690947   2021.07（ eISSN:1664-302X ）

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    Publishing type：Research paper (scientific journal)  

    <italic>Salmonella enterica</italic> subsp. <italic>enterica</italic> serovar Typhimurium sequence type 34 (ST34) and its monophasic variant (<italic>Salmonella</italic> 4,[5],12:i:-) are among the most frequently isolated clones from both humans and animals worldwide. Our previous study demonstrated that <italic>Salmonella</italic> Typhimurium/4,[5],12:i:- strains isolated in Japan could be classified into nine clades and that clade 9 consisted of ST34 strains. In Japan, ST34/clade 9 was first found in the 1990s and has become predominant among food animals in recent years. In the present study, we analyzed the whole genome-based phylogenetic relationships and temporal information of 214 <italic>Salmonella</italic> Typhimurium/4,[5],12:i:- ST34/clade 9 strains isolated from 1998 to 2017 in Japan. The 214 strains were classified into two sublineages: the newly identified clade 9–2 diverged from clade 9 in the early 2000s and has predominated in recent years. Clonally expanding subclades in clades 9–1 or 9–2 lacked Gifsy-1 or HP1 prophages, respectively, and some strains in these subclades acquired plasmids encoding antimicrobial resistance genes. Additional genome reduction around the <italic>fljB</italic> gene encoding the phase 2-H antigen was generated by an IS<italic>26</italic>-mediated deletion adjacent to the transposon in clade 9–2. Although most of the clade 9 strains were isolated from cattle in Japan, the clonally expanding subclades in clade 9–2 (i.e., all and 24% strains of subclades 9–2a and 9–2b, respectively) were isolated from swine. The spread of clade 9 in recent years among food animals in Japan was responsible for the emergence of multiple host-adapted sublineages involving the clonally expanding subclades generated by mobile genetic element-mediated microevolution.

    DOI： 10.3389/fmicb.2021.690947

    PubMed

  • Identification of a recently dominant sublineage in Salmonella 4,[5],12:i:-sequence type 34 isolated from food animals in Japan.

    Arai N, Sekizuka T, Tamamura-Andoh Y, Barco L, Hinenoya A, Yamasaki S, Iwata T, Watanabe-Yanai A, Kuroda M, Akiba M, Kusumoto M

    Frontier in Microbiology   12   2021.07

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    Kind of work：Joint Work  

  • Prevalence, serovar and antimicrobial resistance of non-typhoidal Salmonella in vegetable, fruit and water samples in Ho Chi Minh City, Vietnam.

    Nguyen DTA, Awasthi SP, Hoang PH, Nguyen PD, Hassan J, Hatanaka N, Hinenoya A, Dang CV, Faruque SM, Yamasaki S

    Foodborne Pathog Dis   18 ( 5 )   354 - 363   2021.05

  • Isolation and characterization of Escherichia albertii in poultry at the pre-harvest level. Reviewed

    Atsushi Hinenoya, Xing-Ping Li, Ximin Zeng, Orhan Sahin, Rodney A Moxley, Catherine M Logue, Barbara Gillespie, Shinji Yamasaki, Jun Lin

    Zoonoses and public health   68 ( 3 )   213 - 225   2021.05（ ISSN:1863-1959 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Escherichia albertii, often misidentified as Escherichia coli, has become an emerging foodborne human enteric pathogen. However, the prevalence and major animal reservoirs of this significant pathogen are still not clear. Here, we performed comprehensive microbiological, molecular, comparative genomics and animal studies to understand the status and features of E. albertii in the US domestic and food animals. Although no E. albertii was identified in a total of 1,022 diverse E. coli strains isolated from pets and food animals in a retrospective screening, in a pilot study, E. albertii was successfully isolated from a broiler farm (6 out of 20 chickens). The chicken E. albertii isolates showed clonal relationship as indicated by both pulsed-field gel electrophoresis (PFGE) and whole-genome sequence analysis. The isolated chicken E. albertii displayed multidrug resistance; all the resistance determinants including the extended-spectrum beta-lactamase gene, carried by plasmids, could be conjugatively transferred to E. coli, which was further confirmed by S1-PFGE and Southern hybridization. Whole-genome sequence-based phylogenetic analysis showed the chicken E. albertii strains were phylogenetically close to those of human origins. Challenge experiment demonstrated that the E. albertii strains isolated from human and wild bird could successfully colonize in the chicken intestine. Together, this study, for the first time, reported the isolation of E. albertii in poultry at the pre-hrvest level. The findings from multi-tier characterization of the chicken E. albertii strains indicated the importance of chickens as a reservoir for E. albertii. A large scale of E. albertii survey in poultry production at the pre-harvest level is highly warranted in the future.

    DOI： 10.1111/zph.12812

    PubMed

  • Prevalence, Serovar, and Antimicrobial Resistance of Nontyphoidal Salmonella in Vegetable, Fruit, and Water Samples in Ho Chi Minh City, Vietnam. Reviewed

    Dao Thi Anh Nguyen, Sharda Prasad Awasthi, Phuong Hoai Hoang, Phuc Do Nguyen, Hassan Jayedul, Noritoshi Hatanaka, Atsushi Hinenoya, Chinh Van Dang, Shah M Faruque, Shinji Yamasaki

    Foodborne pathogens and disease   18 ( 5 )   354 - 363   2021.04（ ISSN:1535-3141 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    In this study, we investigated the prevalence, serovar distribution, and antimicrobial resistance pattern of Salmonella isolates from vegetable, fruit, and water samples in Ho Chi Minh City, Vietnam. Salmonella was detected in 75% (30/40), 57.1% (12/21), 17.5% (28/160), and 2.5% (1/40) of river water, irrigation water, vegetable, and ice water samples, respectively. However, no Salmonella was isolated from 160 fruit and 40 tap water samples examined. A total of 102 isolates obtained from 71 samples belonged to 34 different serovars, of which Salmonella Rissen was the most prevalent, followed by Salmonella London, Salmonella Hvittingfoss, and Salmonella Weltevreden. Certain Salmonella serovars such as Newport, Rissen, and Weltevreden were isolated from both vegetable and water samples. Antimicrobial resistance was most commonly observed against tetracycline (35.3%), followed by chloramphenicol (34.3%), ampicillin (31.4%), trimethoprim/sulfamethoxazole (23.5%), and nalidixic acid (10.8%). Of 102 isolates analyzed, 52 (51%) showed resistance to at least 1 antimicrobial class whereas 27 (26.5%) showed multidrug resistant (MDR) phenotype, being resistant to at least three different classes of antimicrobials. Determination of the presence and type of β-lactamase genes showed the cooccurrence of blaTEM-1 and blaCMY-2 in one Salmonella Agona isolate from a river water sample. Taken together, these data indicated that both environmental water and vegetables were contaminated with Salmonella, including MDR strains, and that environmental water used in irrigation might have been the source of Salmonella contamination in the vegetables.

    DOI： 10.1089/fpd.2020.2891

    PubMed

  • Prevalence, O-genotype and Shiga toxin (Stx) 2 subtype of Stx-producing Escherichia coli strains isolated from Argentinean beef cattle. Reviewed

    Kentaro Okuno, Sharda Prasad Awasthi, Germán A Kopprio, Atsushi Iguchi, Noritoshi Hatanaka, Atsushi Hinenoya, Rubén José Lara, Shinji Yamasaki

    The Journal of veterinary medical science   83 ( 4 )   630 - 636   2021.04（ ISSN:0916-7250 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：Domestic journal  

    The aims of this study were to investigate prevalence, O-genotype, and virulence gene profile including Shiga toxin (Stx) 2 gene-subtype of Stx-producing Escherichia coli (STEC) in beef cattle from the Bahía Blanca in Argentina. Rectal swabs were collected from 283 beef cattle in 2012. stx genes were detected in 90 (32%) out of the 283 rectal swabs by stx gene-specific PCR assay. The positive cases were 13 with stx1, 58 with stx2, and 19 with both stx1 and stx2. Among 90 stx gene-positive samples, 45 STEC strains were isolated, which included 3 stx1, 34 stx2, and eight stx1 and stx2 genes positive isolates. O-genotyping grouped 45 STEC strains into 19 different O-genotypes such as Og8, Og145, Og171, Og185 (4 from each), Og22, Og153, Og157 (3 from each) and others. Various stx2 gene-subtypes were identified in 42 STEC strains: 13 positive cases for stx2a, 11 for stx2c, 3 for stx2g, 10 for stx2a and stx2d, 4 for stx2a and stx2c, and 1 for stx2b, stx2c and stx2g. efaI gene, generally prevalent in clinical strains, was detected in relatively high in the STEC strains. These data suggest that stx2a and stx2c were distributed not only in O145 and O157 but also in minor O-genotypes of STEC in Argentina.

    DOI： 10.1292/jvms.21-0002

    PubMed

  • Prevalence, O-genotype and Shiga toxin (Stx) 2 subtype of Stx-producing Escherichia coli strains isolated from Argentinean beef cattle. Reviewed

    Okuno K, Awasthi SP, Kopprio GA, Iguchi A, Hatanaka N, Hinenoya A, Lara RJ, Yamasaki S

    J Vet Med Sci   83 ( 4 )   630 - 636   2021.04

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    Kind of work：Joint Work  

  • Quantification of cholix exotoxin, an ADP-ribosylating factor in Vibrio cholerae strains by developed sandwich bead-ELISA. Reviewed

    Awasthi SP, Chowdhury N, Hatanaka N, Hinenoya A, Ramamurthy T, Asakura M, Yamasaki S

    J Med Microbiol   70 ( 4 )   2021.04

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    Kind of work：Joint Work  

  • Quantification of Vibrio cholerae cholix exotoxin by sandwich bead-ELISA. Reviewed

    Sharda Prasad Awasthi, Nityananda Chowdhury, Noritoshi Hatanaka, Atsushi Hinenoya, Thandavarayan Ramamurthy, Masahiro Asakura, Shinji Yamasaki

    Journal of medical microbiology   70 ( 4 )   2021.04（ ISSN:0022-2615 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Introduction. Cholix toxin (ChxA) is an ADP-ribosylating exotoxin produced by Vibrio cholerae. However, to date, there is no quantitative assay available for ChxA, which makes it difficult to detect and estimate the level of ChxA produced by V. cholerae.Hypothesis/Gap Statement. It is important to develop a reliable and specific quantitative assay to measure the production level of ChxA, which will help us to understand the role of ChxA in V. cholerae pathogenesis.Aim. The aim of this study was to develop a bead-based sandwich ELISA (bead-ELISA) for the quantification of ChxA and to evaluate the importance of ChxA in the pathogenesis of V. cholerae infection.Methodology. Anti-rChxA was raised in New Zealand white rabbits, and Fab-horse radish peroxidase conjugate was prepared by the maleimide method to use in the bead-ELISA. This anti-ChxA bead-ELISA was applied to quantify the ChxA produced by various V. cholerae strains. The production of ChxA was examined in different growth media such as alkaline peptone water (APW), Luria-Bertani broth and AKI. Finally, the assay was evaluated using a mouse lethality assay with representative V. cholerae strains categorized as low to high ChxA-producers based on anti-ChxA bead-ELISA.Results. A sensitive bead-ELISA assay, which can quantify from 0.6 to 60 ng ml-1 of ChxA, was developed. ChxA was mostly detected in the extracellular cell-free supernatant and its production level varied from 1.2 ng ml-1 to 1.6 µg ml-1. The highest ChxA production was observed when V. cholerae strains were cultured in LB broth, but not in APW or AKI medium. The ChxA-producer V. cholerae strains showed 20-80 % lethality and only the high ChxA II-producer was statistically more lethal than a non-ChxA-producer, in the mice model assay. ChxA I and II production levels were not well correlated with mice lethality, and this could be due to the heterogeneity of the strains tested.Conclusion. ChxA I to III was produced mostly extracellularly at various levels depending on strains and culture conditions. The bead-ELISA developed in this study is useful for the detection and quantification of ChxA in V. cholerae strains.

    DOI： 10.1099/jmm.0.001311

    PubMed

  • アルゼンチンの肉用牛より単離された志賀毒素(Stx)産生大腸菌株の保菌率、O-遺伝子型及びStx2サブタイプ(Prevalence, O-genotype and Shiga toxin(Stx) 2 subtype of Stx-producing Escherichia coli strains isolated from Argentinean beef cattle)

    Okuno Kentaro, Awasthi Sharda Prasad, Kopprio German A., Iguchi Atsushi, Hatanaka Noritoshi, Hinenoya Atsushi, Lara Ruben Jose, Yamasaki Shinji

    The Journal of Veterinary Medical Science   83 ( 4 )   630 - 636   2021.04（ ISSN:0916-7250 ）

  • Critical Role of 3'-Downstream Region of pmrB in Polymyxin Resistance in Escherichia coli BL21(DE3). Reviewed

    Fuzhou Xu, Atsushi Hinenoya, Ximin Zeng, Xing-Ping Li, Ziqiang Guan, Jun Lin

    Microorganisms   9 ( 3 )   2021.03

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    Polymyxins, such as colistin and polymyxin B, are the drugs used as a last resort to treat multidrug-resistant Gram-negative bacterial infections in humans. Increasing colistin resistance has posed a serious threat to human health, warranting in-depth mechanistic research. In this study, using a functional cloning approach, we examined the molecular basis of colistin resistance in Escherichia coli BL21(DE3). Five transformants with inserts ranging from 3.8 to 10.7 kb displayed significantly increased colistin resistance, three of which containing pmrB locus and two containing pmrD locus. Stepwise subcloning indicated that both the pmrB with a single G361A mutation and at least a 103 bp downstream region of pmrB are essential for conferring colistin resistance. Analysis of the mRNA level and stability showed that the length of the downstream region drastically affected the pmrB mRNA level but not its half-life. Lipid A analysis, by mass spectrometry, revealed that the constructs containing pmrB with a longer downstream region (103 or 126 bp) have charge-altering l-4-aminoarabinose (Ara4N) and phosphoethanolamine (pEtN) modifications in lipid A, which were not observed in both vector control and the construct containing pmrB with an 86 bp downstream region. Together, the findings from this study indicate that the 3'-downstream region of pmrB is critical for the PmrB-mediated lipid A modifications and colistin resistance in E. coli BL21(DE3), suggesting a novel regulatory mechanism of PmrB-mediated colistin resistance in E. coli.

    DOI： 10.3390/microorganisms9030655

    PubMed

  • Prevalence of mobile colistin resistance (mcr) genes in extended-spectrum β-lactamase-producing Escherichia coli isolated from retail raw foods in Nha Trang, Vietnam. Reviewed

    Phong Quoc Le, Sharda Prasad Awasthi, Noritoshi Hatanaka, Atsushi Hinenoya, Jayedul Hassan, Rabee Alhossiny Ombarak, Atsushi Iguchi, Nga Thuy Thi Tran, Khanh Van Thi Dao, Mai Quang Vien, Huy Xuan Le, Hung Thai Do, Yoshimasa Yamamoto, Shinji Yamasaki

    International journal of food microbiology   346   109164 - 109164   2021.03（ ISSN:0168-1605 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    The aim of the study was to assess the presence of genes in ESBL-producing E. coli (ESBL-Ec) isolated from retail raw food in Nha Trang, Vietnam. A total of 452 food samples comprising chicken (n = 116), pork (n = 112), fish (n = 112) and shrimp (n = 112) collected between 2015 and 2017 were examined for the prevalence of ESBL-Ec. ESBL-Ec were detected in 46.0% (208/452) of retail food samples, particularly in 66.4% (77/116), 55.4% (62/112), 42.0% (47/112) 19.6% (22/112) of chicken, pork, fish and shrimp, respectively. Sixty-five out of the 208 (31.3%) ESBL-Ec isolates were positive for mcr genes including mcr-1, mcr-3 and both mcr-1 and mcr-3 genes in 56/208 (26.9%), 1/208 (0.5%) and 8/208 (3.9%) isolates, respectively. Particularly, there was higher prevalence of mcr-1 in ESBL-Ec isolates from chicken (53.2%, 41/77) in comparison to shrimp (22.7%, 5/22), pork (11.3%, 7/62) and fish (6.4%, 3/47). mcr-3 gene was detected in co-existence with mcr-1 in ESBL-Ec isolates from shrimp (9.1%, 2/22), pork (8.1%, 5/62) and fish (2.1%, 1/47) but not chicken. The 65 mcr-positive ESBL-Ec (mcr-ESBL-Ec) were colistin-resistant with the MICs of 4-8 μg/mL. All mcr-3 gene-positive isolates belonged to group A, whereas phylogenetic group distribution of isolates harboring only mcr-1 was B1 (44.6%), A (28.6%) and D (26.8%). PFGE analysis showed diverse genotypes, although some isolates demonstrated nearly clonal relationships. S1-PFGE and Southern hybridization illustrated that the mcr-1 and mcr-3 genes were located either on chromosomes or on plasmids. However, the types of mcr genes were harbored on different plasmids with varied sizes of 30-390 kb. Besides, the ESBL genes of CTX-M-1 or CTX-M-9 were also detected to be located on plasmids. Noteworthy, co-location of CTX-M-1 with mcr-1 or mcr-3 genes on the same plasmid was identified. The conjugation experiment indicated that the mcr-1 or mcr-3 was horizontally transferable. All mcr-ESBL-Ec isolates were multidrug resistance (resistance to ≥3 antimicrobial classes). Moreover, β-Lactamase-encoding genes of the CTX-M-1 (78.5%), CTX-M-9 (21.5%), TEM (61.5%) groups were found in mcr-ESBL-Ec. The astA gene was detected in 27 (41.5%) mcr-ESBL-Ec isolates demonstrating their potential virulence. In conclusion, mcr-1 and mcr-3 genes existed individually or concurrently in ESBL-Ec isolates recovered from retail raw food in Nha Trang city, which might further complicate the antimicrobial-resistant situation in Vietnam, and is a possible health risk for human.

    DOI： 10.1016/j.ijfoodmicro.2021.109164

    PubMed

  • Critical role of 3'-downstream region of pmrB in polymyxin resistance in Escherichia coli BL21(DE3). Reviewed

    Fuzhou X, Hinenoya A, Zeng X, Li X-P, Guan Z, Lin J

    Microorganisms   9 ( 3 )   2021.03

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  • Prevalence of mobile colistin resistance (mcr) genes in extended-spectrum β-lactamase-producing Escherichia coli isolated from retail raw foods in Nha Trang, Vietnam.

    Le PQ, Awasthi SP, Hatanaka N, Hinenoya A, Hassan J, Ombarak RA, Iguchi A, Tran NTT, Dao KVT, Vien MQ, Le HX, Do HT, Yamamoto Y, Yamasaki S

    Int J Food Microbiol   346   2021.03

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    Kind of work：Joint Work  

  • Isolation and characterization of Escherichia albertii in poultry at the pre-harvest level. Reviewed

    Hinenoya A, Li X-P, Zeng X, Sahin O, Moxley RA, Logue CM, Gillespie B, Yamasaki S, Lin J

    Zoonoses and Public Health   2021.02

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    Kind of work：Joint Work  

  • Accurate identification of Escherichia albertii by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Reviewed

    Hatanaka N, Awasthi SP, Hinenoya A, Ueda O, Yamasaki S

    Journal of Microbiological Methods   173   2020.06

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    Kind of work：Joint Work  

  • Prevalence of Escherichia albertii in raccoons (Procyon lotor) in Japan Reviewed

    Hinenoya A, Nagano K, Awasthi SP, Hatanaka N, Yamasaki S

    Emerging and Infectious Diseases   26 ( 6 )   1304 - 1307   2020.06

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  • Prevalence of Escherichia albertii in Raccoons (Procyon lotor), Japan. Reviewed

    Atsushi Hinenoya, Keigo Nagano, Sharda P Awasthi, Noritoshi Hatanaka, Shinji Yamasaki

    Emerging infectious diseases   26 ( 6 )   1304 - 1307   2020.06（ ISSN:1080-6040 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Natural reservoirs of Escherichia albertii remain unclear. In this study, we detected E. albertii by PCR in 248 (57.7%) of 430 raccoons from Osaka, Japan, and isolated 143 E. albertii strains from the 62 PCR-positive samples. These data indicate that raccoons could be a natural reservoir of E. albertii in Japan.

    DOI： 10.3201/eid2606.191436

    PubMed

  • Chlorous acid is a more potent antibacterial agent than sodium hypochlorite against Campylobacter. Reviewed

    Hatanaka N, Awasthi SP, Goda H, Kawata H, Uchino Y, Kubo T, Aoki S, Hinenoya A, Yamasaki S

    Food Control   111   2020.05

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    Kind of work：Joint Work  

  • Development of XRM-MacConkey agar selective medium for the isolation of Escherichia albertii. Reviewed

    Hinenoya A, Nagano K, Okuno K, Nagita A, Hatanaka N, Awasthi SP, Yamasaki S

    Diagnostic Microbiology and Infectious Disease   97 ( 1 )   2020.05

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  • Development of XRM-MacConkey agar selective medium for the isolation of Escherichia albertii. Reviewed

    Atsushi Hinenoya, Keigo Nagano, Kentaro Okuno, Akira Nagita, Noritoshi Hatanaka, Sharda Prasad Awasthi, Shinji Yamasaki

    Diagnostic microbiology and infectious disease   97 ( 1 )   115006 - 115006   2020.05（ ISSN:0732-8893 ）

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    International / domestic magazine：International journal  

    Escherichia albertii has increasingly been recognized as an emerging pathogen. However, lack of selective medium for E. albertii is the bottleneck for clinical and epidemiological investigations. In this study, a selective medium for E. albertii named XRM-MacConkey agar, which is modified MacConkey agar supplemented with xylose (X), rhamnose (R), and melibiose (M) instead of lactose, was developed and evaluated. All 49 E. albertii and 6 different species out of 23 grew as colorless colonies, whereas 17 remaining species grew as red colonies. Detection limit of E. albertii by this medium was 105 CFU/g stool when examined with spiked healthy human stool. Furthermore, colorless colonies on XRM-MacConkey agar obtained from 7 E. albertii-positive diarrheal stools were consistently E. albertii. In contrast, 57%, 18%, and 36% colorless colonies on MacConkey, DHL, and mEA agars, respectively, were non-E. albertii. We concluded that XRM-MacConkey agar could specifically be used for isolation of E. albertii.

    DOI： 10.1016/j.diagmicrobio.2020.115006

    PubMed

  • Chlorous acid is a more potent antibacterial agent than sodium hypochlorite against Campylobacter Reviewed

    Noritoshi Hatanaka, Sharda Prasad Awasthi, Hisataka Goda, Hiroyuki Kawata, Yuzuru Uchino, Takahiro Kubo, Shigeru Aoki, Atsushi Hinenoya, Shinji Yamasaki

    Food Control   111   2020.05（ ISSN:0956-7135 ）

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    Publishing type：Research paper (scientific journal)  

    © 2019 Elsevier Ltd Foodborne disease caused by campylobacters is one of the major global problems for food safety. Infection source of Campylobacter to human is mainly through contaminated meat particularly chicken. Contamination of meat with Campylobacter usually occurs during processing at slaughterhouse and to prevent such contaminations, sodium hypochlorite is commonly used. However, it is well known that bactericidal activity of sodium hypochlorite becomes weak under organic matter rich conditions. In this study, we compared the strength of bactericidal activity of chlorous acid and sodium hypochlorite against Campylobacter jejuni and Campylobacter coli strains under organic matter rich conditions. Bactericidal activity against 5 representative C. jejuni and C. coli strains in chicken juice (an organic matter rich condition) showed that minimum concentration of chlorous acid required for complete killing of C. jejuni and C. coli cells is 200–400 ppm while that of sodium hypochlorite is 2,000 to 4,000 ppm. Similar results were obtained by using Bolton broth. Furthermore, it was observed that 400 ppm of chlorous acid but not 400 ppm of sodium hypochlorite is highly effective in killing of 25 different Campylobacter strains (12 C. jejuni and 13 C. coli strains) under the same conditions. To determine whether 400 ppm of chlorous acid treatment had killed bacterial cells or induced them into viable but non-culturable (VBNC) state, live and dead cell assay using DAPI and propidium iodide fluorescent dyes was done. Such assay clearly indicated that Campylobacter cells were indeed killed and not induced to VBNC state. Moreover, SDS-PAGE analysis of whole-cell lysates of campylobacters indicated distinct effects in protein profiles of chlorous acid but not sodium hypochlorite treated cells. The results strongly suggest that chlorous acid could efficiently kill C. jejuni and C. coli cells with much lower concentration than sodium hypochlorite and the bactericidal mechanisms of chlorous acid may be due to damages of bacterial proteins. Thus, chlorous acid could be a better disinfectant in chicken slaughtering and processing to kill campylobacters and prevent contamination.

    DOI： 10.1016/j.foodcont.2019.107046

  • Accurate identification of Escherichia albertii by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Reviewed

    Noritoshi Hatanaka, Sharda Prasad Awasthi, Atsushi Hinenoya, Osamu Ueda, Shinji Yamasaki

    Journal of microbiological methods   173   105916 - 105916   2020.04（ ISSN:0167-7012 ）

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    International / domestic magazine：International journal  

    A specific identification protocol for Escherichia albertii by using a MALDI-TOF/MS method was developed. For this purpose, a novel database was established which can differentiate E. albertii from E. coli by combining the mass spectra obtained from 58 E. albertii and 36 E. coli strains.

    DOI： 10.1016/j.mimet.2020.105916

    PubMed

  • Development of a specific cytolethal distending toxin (cdt) gene (Eacdt)-based PCR assay for the detection of Escherichia albertii. Reviewed

    Hinenoya A, Ichimura H, Yasuda N, Harada S, Yamada K, Suzuki M, Iijima Y, Nagita A, Albert MJ, Hatanaka N, Awasthi SP, Yamasaki S

    Diagn Microbiol Infect Dis 雑誌 ELSEVIER   95 ( 2 )   119 - 124   2019.10

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    Kind of work：Single Work  

  • Development of a specific cytolethal distending toxin (cdt) gene (Eacdt)-based PCR assay for the detection of Escherichia albertii. Reviewed

    Atsushi Hinenoya, Hidetoshi Ichimura, Noritomo Yasuda, Seiya Harada, Kazuhiro Yamada, Masahiro Suzuki, Yoshio Iijima, Akira Nagita, M John Albert, Noritoshi Hatanaka, Sharda Prasad Awasthi, Shinji Yamasaki

    Diagnostic microbiology and infectious disease   95 ( 2 )   119 - 124   2019.10（ ISSN:0732-8893 ）

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    International / domestic magazine：International journal  

    Many Escherichia albertii isolates, an emerging pathogen of human and birds, might have been misidentified due to the difficulty of differentiating this bacterium from Escherichia coli and Shigella spp. by routine biochemical tests, resulting in underestimation of E. albertii infections. We have developed a polymerase chain reaction (PCR) assay that targets E. albertii cytolethal distending toxin (Eacdt) genes, which include the genes previously identified as Escherichia coli cdt-II. This assay could generate a single 449-bp PCR product in each of 67 confirmed E. albertii strains but failed to produce PCR product from any of the tested non-E. albertii enteric strains belonging to 37 different species, indicating 100% sensitivity and specificity of the PCR assay. The detection limit was 10 CFU per PCR tube and could detect 105 CFU E. albertii per gram of spiked healthy human stool. The Eacdt gene-based PCR could be useful for simple, rapid, and accurate detection and identification of E. albertii.

    DOI： 10.1016/j.diagmicrobio.2019.04.018

    PubMed

  • Serotypes, pathogenic potential and antimicrobial resistance of Escherichia coli isolated from subclinical bovine mastitis milk samples in Egypt. Reviewed

    Ombarak RA, Zayda MG, Awasthi SP, Hinenoya A, Yamasaki S

    Jpn J Infect Dis 雑誌 国立感染症研究所   72 ( 5 )   337 - 339   2019.09

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  • Extended-spectrum beta-lactamase-producing Escherichia coli harboring sul and mcr-1 genes isolates from fish gut contents in the Mekong delta, Vietnam. Reviewed

    Tran HTT, Nakayama T*, Huyen HM, Harada K, Hinenoya A, Phuong NT, Yamamoto Y.

    Letters in Applied Microbiology   71   78 - 85   2019.09

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  • Serotypes, Pathogenic Potential, and Antimicrobial Resistance of Escherichia coli Isolated from Subclinical Bovine Mastitis Milk Samples in Egypt. Reviewed

    Ombarak RA, Zayda MG, Awasthi SP, Hinenoya A, Yamasaki S

    Japanese journal of infectious diseases   72 ( 5 )   337 - 339   2019.09（ ISSN:1344-6304 ）

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  • エジプトにおける無症状性ウシ乳房炎の牛乳試料から分離された大腸菌の血清型、病原性、抗菌薬耐性(Serotypes, Pathogenic Potential, and Antimicrobial Resistance of Escherichia coli Isolated from Subclinical Bovine Mastitis Milk Samples in Egypt)

    Ombarak Rabee Alhossiny, Zayda Mahmoud Gamaleldin, Awasthi Sharda Prasad, Hinenoya Atsushi, Yamasaki Shinji

    Japanese Journal of Infectious Diseases   72 ( 5 )   337 - 339   2019.09（ ISSN:1344-6304 ）

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    無症状性乳房炎(SCM)のウシ由来の牛乳試料の9.3%から大腸菌が分離されたことから、これら大腸菌の血清型と病原性および抗菌薬耐性(AMR)について検討した。分離された大腸菌(n=14)の血清型は、O55:H7(n=2)、O111:H4(n=2)、O127:H6(n=2)、O128:HUT(n=2)、O26:HUT(n=1)、O44:H18(n=1)、O114:H21(n=1)、O86:HUT(n=1)、O124:HUT(n=1)、およびO128:H7(n=1)であることが判明した。また、93%(13/14)から潜在的病原性が検出され、少なくとも一つの病原性遺伝子を有していた。さらに、71%(10/14)は試験した15種の抗菌薬の少なくとも一つに耐性を示し、40%(4/14)は多剤耐性であり、基質特異性拡張型βラクタマーゼを産生する菌も見出された。以上から、SCMが抗菌薬耐性を示す病原性大腸菌株の源となる可能性が示唆された。

  • Salmonella genomic island 3 is an integrative and conjugative element and contributes to copper and arsenic tolerance of Salmonella enterica. Reviewed

    Arai N, Sekizuka T, Tamamura Y, Kusumoto M, Hinenoya A, Yamasaki S, Iwata T, Watanabe-Yanai A, Kuroda M, Akiba M

    Appl. Environ Microbiol 雑誌 American Society for Microbiology   63 ( 9 )   pii: e00429 - 19   2019.08

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  • Salmonella Genomic Island 3 Is an Integrative and Conjugative Element and Contributes to Copper and Arsenic Tolerance of Salmonella enterica Reviewed

    Nobuo Arai, Tsuyoshi Sekizuka, Yukino Tamamura, Masahiro Kusumoto, Atsushi Hinenoya, Shinji Yamasaki, Taketoshi Iwata, Ayako Watanabe-Yanai, Makoto Kuroda, Masato Akiba

    Antimicrobial Agents and Chemotherapy   63 ( 9 )   2019.06（ ISSN:0066-4804 ） （ eISSN:1098-6596 ）

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    Publishing type：Research paper (scientific journal)  

    <title>ABSTRACT</title>
    <italic>Salmonella</italic> genomic island 3 (SGI3) was first described as a chromosomal island in <italic>Salmonella</italic> 4,[5],12:i:–, a monophasic variant of <named-content content-type="genus-species">Salmonella enterica</named-content> subsp. <italic>enterica</italic> serovar Typhimurium. The SGI3 DNA sequence detected from <italic>Salmonella</italic> 4,[5],12:i:– isolated in Japan was identical to that of a previously reported one across entire length of 81 kb. SGI3 consists of 86 open reading frames, including a copper homeostasis and silver resistance island (CHASRI) and an arsenic tolerance operon, in addition to genes related to conjugative transfer and DNA replication or partitioning, suggesting that the island is a mobile genetic element. We successfully selected transconjugants that acquired SGI3 after filter-mating experiments using the <named-content content-type="genus-species">S. enterica</named-content> serovars Typhimurium, Heidelberg, Hadar, Newport, Cerro, and Thompson as recipients. Southern blot analysis using I-CeuI-digested genomic DNA demonstrated that SGI3 was integrated into a chromosomal fragment of the transconjugants. PCR and sequencing analysis demonstrated that SGI3 was inserted into the 3′ end of the tRNA genes <italic>pheV</italic> or <italic>pheR</italic>. The length of the target site was 52 or 55 bp, and a 55-bp <italic>attI</italic> sequence indicating generation of the circular form of SGI3 was also detected. The transconjugants had a higher MIC against CuSO<sub>4</sub> compared to the recipient strains under anaerobic conditions. Tolerance was defined by the <italic>cus</italic> gene cluster in the CHASRI. The transconjugants also had distinctly higher MICs against Na<sub>2</sub>HAsO<sub>4</sub> compared to recipient strains under aerobic conditions. These findings clearly demonstrate that SGI3 is an integrative and conjugative element and contributes to the copper and arsenic tolerance of <named-content content-type="genus-species">S. enterica</named-content>.

    DOI： 10.1128/aac.00429-19

    PubMed

  • Evaluation of the GeneFields® EHEC/SS PCR dipstick DNA chromatography kit for the detection of enteric bacterial pathogens in stool specimens of healthy humans. Reviewed

    Maruoka H, Hinenoya A, Yasuda N, Takeda A, Inoue S, Sumi T, Koitabashi K, Yasue H, Kogou K, Yamasaki S

    J Microbiol Methods 雑誌 ELSEVIER   161   111 - 117   2019.06

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  • Evaluation of the GeneFields® EHEC/SS PCR dipstick DNA chromatography kit for the detection of enteric bacterial pathogens in stool specimens of healthy humans. Reviewed

    Maruoka H, Hinenoya A, Yasuda N, Takeda A, Inoue S, Sumi T, Koitabashi K, Yasue H, Kogou K, Yamasaki S

    Journal of microbiological methods   161   111 - 117   2019.06（ ISSN:0167-7012 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    We developed a new GeneFields® EHEC/SS PCR dipstick DNA chromatography kit for the simultaneously detection of invA, ipaH, and stx genes in Salmonella enterica (56 strains), Shigella spp. (44), and enterohemorrhagic Escherichia coli (EHEC) (28), respectively, and evaluated the sensitivity and specificity with other bacteria (57) by this kit. The sensitivity and specificity were 100%, respectively. The detection limit of various methods was determined using 5% (w/v) stool suspensions spiked with each bacterium. The detection limit of the GeneFields® EHEC/SS kit ranged from approximately 102-103 CFU/g. Additionally, the relative sensitivities and specificities of the GeneFields® EHEC/SS kit vs two commercially available real-time PCR kits were >85.0% and >90.0%, respectively. These results indicate that the GeneFields® EHEC/SS kit can be used for genetic screening of S. enterica, Shigella spp., and EHEC in human stool specimens with sensitivities and specificities similar to those of the commercially available real-time PCR kits.

    DOI： 10.1016/j.mimet.2019.04.016

    PubMed

  • Comparison of Established PCR Assays for Accurate Identification of Campylobacter jejuni and Campylobacter coli. Reviewed

    S M Lutful Kabir, Nityananda Chowdhury, Masahiro Asakura, Sachi Shiramaru, Ken Kikuchi, Atsushi Hinenoya, Sucharit Basu Neogi, Shinji Yamasaki

    Japanese journal of infectious diseases   72 ( 2 )   81 - 87   2019.03（ ISSN:1344-6304 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：Domestic journal  

    Proper surveillance of Campylobacter jejuni and Campylobacter coli, major pathogens associated with human gastroenteritis, is necessary to tackle the increasing disease burden. To detect these pathogenic species, a variety of PCR assays have been developed. This study examined the sensitivity and specificity of 12 PCR assays targeting 23S rRNA, ceuE, lpxA, hipO, mapA, ask, and cdt genes of C. jejuni and C. coli. The sensitivities of PCR assays were 85.2-100%, and 97-100%, and the specificities were 90.5-100%, and 94.3-100% for the tested C. jejuni (n = 61) and C. coli (n = 33) strains, respectively. Two PCR assays, targeting cdtC and hipO genes, were found to be 100% sensitive and/or specific for all C. jejuni strains, while 3 assays, targeting cdtB, cdtA, and ask genes, were 100% sensitive and/or specific for C. coli strains. However, PCR assays for hipO and ask genes are problematic to conduct simultaneously due to the differences in PCR conditions. Overall, multiplex PCR assays targeting cdtC and cdtB genes, encoding 2 subunits of the same toxin, were concluded to be the most reliable. The results of this study would aid in proper surveillance of C. jejuni and C. coli and adopting intervention strategies in the near future.

    DOI： 10.7883/yoken.JJID.2018.340

    PubMed

  • Phenotypic and molecular characterization of Escherichia albertii: Further surrogates to avoid potential laboratory misidentification. Reviewed

    Hinenoya A, Ichimura H, Awasthi SP, Yasuda N, Yatsuyanagi J, Yamasaki S

    Int J Med Microbiol 雑誌 ELSEVIER   309 ( 2 )   108 - 115   2019.03

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  • Comparison of established PCR assays for accurate identification of Campylobacter jejuni and Campylobacter coli. Reviewed

    Kabir SML, Chowdhury N, Asakura M, Shiramaru S, Kikuchi K, Hinenoya A, Neogi SB, Yamasaki S

    Jpn J Infect Dis 雑誌 国立感染症研究所   72 ( 2 )   81 - 87   2019.03

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  • Campylobacter jejuniとCampylobacter coliの正確な同定に用いられるPCRアッセイの比較(Comparison of Established PCR Assays for Accurate Identification of Campylobacter jejuni and Campylobacter coli)

    Kabir S.M. Lutful, Chowdhury Nityananda, Asakura Masahiro, Shiramaru Sachi, Kikuchi Ken, Hinenoya Atsushi, Neogi Sucharit Basu, Yamasaki Shinji

    Japanese Journal of Infectious Diseases   72 ( 2 )   81 - 87   2019.03（ ISSN:1344-6304 ）

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    Campylobacter jejuniとCampylobacter coliの同定に用いられる12種類のPCRアッセイ法の感度と特異度を比較検討した。日本国内で分離されたカンピロバクター菌のうち、C.jejuni 58株、C.coli 31株を分析対象とした。検討の結果、PCRアッセイによって菌検出の有用性に関して大きな差が認められた。C.jejuniの検出に際して、ceuE遺伝子ベースのアッセイでは感度は85%にとどまり、cdtC、hipO、IpxAの各遺伝子をベースとしたアッセイでは感度100%、cdtA、cdtB、mapA、23S rRNA遺伝子ベース解析では感度は92〜97%を示していた。一方、C.coliに対しては各PCRアッセイの検出率は良好であり、ceuEとcdtA遺伝子ベース解析で感度が97%を示したほか、各アッセイの感度は100%であった。cdtA、cdtBおよびcdtCをベースとする多重PCRアッセイでは、C.jejuniとC.coliの同時検出における感度は100%を示し、hipO、ask、ceuEを用いた多重アッセイでも同様の成績が得られたが、IpxAと23S rRNAを用いたアッセイでは偽陽性が認められ感度は低くなっていた。今回検討したPCRアッセイのうち、cdtCとcdtBの両遺伝子を標的とする多重PCRアッセイが最も信頼性が高い検出法であると考えられた。

  • Phenotypic and molecular characterization of Escherichia albertii: Further surrogates to avoid potential laboratory misidentification. Reviewed

    Hinenoya A, Ichimura H, Awasthi SP, Yasuda N, Yatsuyanagi J, Yamasaki S

    International journal of medical microbiology : IJMM   309 ( 2 )   108 - 115   2019.03（ ISSN:1438-4221 ）

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  • Development of a bivalent food poisoning vaccine: augmented antigenicity of the C-terminus of Clostridium perfringens enterotoxin by fusion with the B subunit of Escherichia coli Shiga toxin 2. Reviewed

    Hosomi K, Hinenoya A, Suzuki H, Nagatake T, Nishino T, Tojima Y, Hirata SI, Matsunaga A, Kondoh M, Yamasaki S, Kunisawa J

    International immunology   31 ( 2 )   91 - 100   2019.03（ ISSN:0953-8178 ） （ eISSN:1460-2377 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/intimm/dxy071

    PubMed

  • Development of a bivalent food poisoning vaccine: augmented antigenicity of the C-terminus of Clostridium perfringens enterotoxin by fusion with the B subunit of Escherichia coli Shiga toxin 2. Reviewed

    Hosomi K, Hinenoya A, Suzuki H, Nagatake T, Nishino T, Tojima Y, Hirata SI, Matsunaga A, Kondoh M, Yamasaki S, Kunisawa J

    Int Immunol 雑誌 Oxford Academic   31 ( 2 )   91 - 100   2019.02

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  • Development of a multiplex PCR assay for the detection of major virulence genes in Vibrio cholerae including non-O1 and non-O139 serogroups. Reviewed

    Awasthi SP, Chowdhury N, Neogi SB, Hinenoya A, Hatanaka N, Chowdhury G, Ramamurthy T, Yamasaki S

    J Microbiol Methods 雑誌 ELSEVIER   157   54 - 58   2019.02

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  • Development of a multiplex PCR assay for the detection of major virulence genes in Vibrio cholerae including non-O1 and non-O139 serogroups.

    Awasthi SP, Chowdhury N, Neogi SB, Hinenoya A, Hatanaka N, Chowdhury G, Ramamurthy T, Yamasaki S

    Journal of microbiological methods   157   54 - 58   2019.02（ ISSN:0167-7012 ）

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  • Characterization of Vibrio cholerae isolates from two distinct Kenyan cholera outbreaks in 2007 and 2009 Reviewed

    S. P. Awasthi, S. M. Saidi, N. Chowdhury, M. Asakura, P. H. Hoang, A. Hinenoya, D. Motooka, S. Nakamura, Y. Iijima, S. Yamasaki

    2019.02

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    Publishing type：Research paper (international conference proceedings)  

  • 2価食中毒ワクチンの開発　志賀毒素2型産生大腸菌Bサブユニットとの融合によりウエルシュ菌エンテロトキシンC末端の抗原性が増強される(Development of a bivalent food poisoning vaccine: augmented antigenicity of the C-terminus of Clostridium perfringens enterotoxin by fusion with the B subunit of Escherichia coli Shiga toxin 2)

    Hosomi Koji, Hinenoya Atsushi, Suzuki Hidehiko, Nagatake Takahiro, Nishino Tomomi, Tojima Yoko, Hirata So-ichiro, Matsunaga Ayu, Kondoh Masuo, Yamasaki Shinji, Kunisawa Jun

    International Immunology   31 ( 2 )   91 - 100   2019.02（ ISSN:0953-8178 ）

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    今回、ウエルシュ菌や志賀毒素(Stx)産生大腸菌(STEC)感染により誘発される重篤な全身症状や、致死性ウエルシュ菌エンテロトキシン(CPE)およびStxに対し感染防御効果を示す2価ワクチンを開発した。なお、開発したワクチンは、志賀毒素2型のBサブユニット(Stx2B)とCPEのC末端(C-CPE)を融合(Stx2B-C-CPE)させて作製し、C-CPEの抗原性を増強させたことを特徴とした。本ワクチンをStx2B-C-CPEで免疫したマウスに接種させ、有効性を評価した結果、中和試験により、高レベルのC-CPE特異的IgGおよび相当量のStx2B特異的IgGが誘導され、これらの抗体応答が最低48週間継続したことから、本ワクチンが長期間の防御免疫を誘導する可能性が示唆された。さらに、Stx2B-C-CPEを用いた免疫応答では、Stx2B刺激によりStx2B特異的T細胞が誘導され、IgMからIgGへ、Stx2B-およびC-CPE-特異的B細胞において、免疫グロブリン分子のクラススイッチが促進されるメカニズムも確認された。以上の所見から、ウエルシュ菌およびSTEC感染に対する本ワクチンの有効性が立証された。

  • Novel cholera toxin variant and ToxT regulon in environmental Vibrio mimicus strains: potential resources for the evolution of Vibrio cholerae hybrid strains. Reviewed

    Neogi SB, Chowdhury N, Awasthi SP, Asakura M, Okuno K, Mahmud ZH, Islam MS, Hinenoya A, Nair GB, Yamasaki S

    Appl Environ Microbiol 雑誌 American Society for Microbiology   85 ( 3 )   pii: e01977 - 18   2019.01

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  • Novel cholera toxin variant and ToxT regulon in environmental <i>Vibrio mimicus</i> strains: potential resources for the evolution of <i>Vibrio cholerae</i> hybrid strains. Reviewed

    Neogi SB, Chowdhury N, Awasthi SP, Asakura M, Okuno K, Mahmud ZH, Islam MS, Hinenoya A, Nair GB, Yamasaki S

    Applied and environmental microbiology   85 ( 3 )   2019.01（ ISSN:0099-2240 ）

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  • Extended-spectrum beta-lactamase-producing Escherichia coli harbouring sul and mcr-1 genes isolates from fish gut contents in the Mekong Delta, Vietnam Reviewed

    T. T.T. Hoa, T. Nakayama, H. M. Huyen, K. Harada, A. Hinenoya, N. T. Phuong, Y. Yamamoto

    Letters in Applied Microbiology   2019（ ISSN:0266-8254 ） （ eISSN:1472-765X ）

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    Publishing type：Research paper (scientific journal)  

    © 2019 The Society for Applied Microbiology This study investigated the existence of sulfonamides and colistin resistance genes among extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli recovered from fish gut in Vietnam and evaluated the susceptibility patterns of the ESBL-producing E. coli to relevant antimicrobials. A total of 88 ESBL-producing E. coli isolates were analysed for the presence of the ESBLs, sul (1, 2, 3) and mcr (1–3) genes by PCR. Antimicrobial resistance phenotypes of isolates were determined by disc diffusion. Results showed that: (i) A high prevalence of 94·3% of sulfonamide resistance was observed in 88 isolates. Moreover, the existence of 2·3% of ESBL-producing E. coli harbouring mcr-1 gene were detected; (ii) The phylogenetic types A and B1 were most frequent, and the blaCTX-M group1 and blaTEM genes encoding ESBL were detected in 47·7% of the isolates; (iii) ESBL-producing E. coli harbouring mcr-1 gene exhibited resistance to 11 antibiotics. The existence of mcr-1 and sul1,2,3 genes and the extremely high level of multiple drug resistance in all ESBL-producing E. coli isolates obtained from sampled fish in Vietnam is a major concern. Therefore, it is imperative to monitor ESBL-producing E. coli in the river waters of Vietnam.

    DOI： 10.1111/lam.13222

    PubMed

  • Development of a multiplex PCR targeting eae, stx and cdt genes in genus Escherichia and detection of a novel cdtB gene in Providencia rustigianii. Reviewed

    Hassan J, Awasthi SP, Hatanaka N, Okuno K, Hoang PH, Nagita A, Hinenoya A, Yamasaki S

    Pathogens and Disease 雑誌 Oxford Academic   76 ( 9 )   2018.12

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  • MCR-1 confers cross-resistance to Bacitracin, a widely used in-feed antibiotic. Reviewed

    Xu F, Zeng X, Hinenoya A, Lin J

    mSphere 雑誌 American Society for Microbiology   3 ( 5 )   pii: e00411 - 18   2018.09

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  • Phylogenetic characterization of Salmonella enterica serovar Typhimurium and its monophasic variant isolated from food animals in Japan revealed replacement of major epidemic clones in the last four decades. Reviewed

    Arai N, Sekizuka T, Tamamura Y, Tanaka K, Barco L, Izumiya H, Kusumoto M, Hinenoya A, Yamasaki S, Iwata T, Watanabe A, Kuroda M, Uchida I, Akiba M

    J Clin Microbiol 雑誌 American Society for Microbiology   56 ( 5 )   pii: e01758 - 17   2018.04

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  • Prevalence and characterization of extended-spectrum β-lactamases-producing Escherichia coli in domestic and imported chicken meats in Japan. Reviewed

    Nahar A, Awasthi SP, Hatanaka N, Okuno K, Hoang PH, Hassan J, Hinenoya A, Yamasaki S

    J Vet Med Sci 雑誌 Japanese Society of Veterinary Science   80 ( 3 )   510 - 517   2018.03

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  • Isolation and molecular characterization of extended-spectrum β-lactamase (ESBL) producing Escherichia coli from industrial food animals in Mekong Delta, Vietnam. Reviewed

    Hinenoya A, Tran Thi Thu S, Nguyen NT, Nguyen HC, Le Nguyen DD, Hoang PH, Awasthi SP, Hassan J, Sumimura Y, Yamamoto Y, Yamasaki S

    Jpn J Vet Res 雑誌 Faculty of Veterinary Medicine, Hokkaido University   66 ( 1 )   1 - 12   2018.02

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  • Prevalence and molecular characterization of antimicrobial resistance in Escherichia coli isolated from raw milk and raw milk cheese in Egypt. Reviewed

    Ombarak RA, Hinenoya A, Elbagory AM, Yamasaki S

    J Food Prot 雑誌 International Association for Food Protection   81 ( 2 )   226 - 232   2018.02

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  • Association of cytolethal distending toxin-II gene-positive Escherichia coli with Escherichia albertii, an emerging zoonotic pathogen. Reviewed

    Hinenoya A, Yasuda N, Mukaizawa N, Sheikh S, Niwa Y, Awasthi SP, Asakura M, Tsukamoto T, Nagita A, Albert MJ, Yamasaki S

    Int J Med Microbiol 雑誌 ELSEVIER   307 ( 8 )   564 - 571   2017.12

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  • High prevalence of Campylobacter ureolyticus in stool specimens of children with diarrhea in Japan. Reviewed

    Hatanaka N, Shimizu A, Somroop S, Li Y, Asakura M, Nagita A, Awasthi SP, Hinenoya A, Yamasaki S

    Jpn J Infect Dis 雑誌 国立感染症研究所   70 ( 4 )   455 - 457   2017.07

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  • Isolation and characterization of an Escherichia albertii producing three different toxins from a child with diarrhea. Reviewed

    Hinenoya A, Yasuda N, Hibino T, Shima A, Nagita A, Tsukamoto T, Yamasaki S

    Jpn J Infect Dis 雑誌 国立感染症研究所   70 ( 3 )   252 - 257   2017.05

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  • Water metagenomic analysis reveals low bacterial diversity and the presence of antimicrobial residues and resistance genes in a river containing wastewater from backyard aquacultures in the Mekong Delta, Vietnam. Reviewed

    Nakayama T, Tuyet Hoa TT, Harada K, Warisaya M, Asayama M, Hinenoya A, Lee JW, Phu TM, Ueda S, Sumimura Y, Hirata K, Phuong NT, Yamamoto Y.

    Environ Pollut 雑誌 ELSEVIER   222   294 - 306   2017.03

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  • Antimicrobial resistance profiles and molecular characterization of Escherichia coli strains isolated from healthy adults in Ho Chi Minh City, Vietnam. Reviewed

    Hoang PH, Awasthi SP, DO Nguyen P, Nguyen NL, Nguyen DT, LE NH, VAN Dang C, Hinenoya A, Yamasaki S

    J Vet Med Sci 雑誌 Japanese Society of Veterinary Science   79 ( 3 )   479 - 485   2017.03

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  • Campylobacter upsaliensis isolated from dog produces high titer of cytolethal distending toxin. Reviewed

    Somroop S, Hatanaka N, Awasthi SP, Okuno K, Asakura M, Hinenoya A, Yamasaki S

    J Vet Med Sci 雑誌 Japanese Society of Veterinary Science   79 ( 3 )   683 - 691   2017.03

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  • A PCR-RFLP assay to detect and type cytolethal distending toxin (cdt) genes in Campylobacter hyointestinalis. Reviewed

    Hatanaka N, Kamei K, Somroop S, Awasthi SP, Asakura M, Misawa N, Hinenoya A, Yamasaki S

    J Vet Med Sci 雑誌 Japanese Society of Veterinary Science   79 ( 2 )   336 - 342   2017.02

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  • Anethole inhibits growth of recently emerged multidrug resistant toxigenic Vibrio cholerae O1 El Tor variant strains in vitro Reviewed

    M.S. Zahid, S.P. Awasthi, A. Hinenoya, S. Yamasaki

    J Vet Med Sci 雑誌 Japanese Society of Veterinary Science   77 ( 5 )   535 - 540   2015.06

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  • Prevalence of Providencia strains among patients and retail meats in Thailand Reviewed

    A. Shima, A. Hinenoya, W. Samosornsuk, S. Samosornsuk, S. Yamasaki

    Jpn J Infect Dis 雑誌 国立感染症研究所   2015

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  • Chlorine dioxide is a superior disinfectant against multi-drug resistant Staphylococcus aureus, Pseudomonas aeruginosa and Acinetobacter baumannii Reviewed

    A. Hinenoya, S.P. Awasthi, N. Yasuda, A. Shima, H. Morino, T. Koizumi, T. Fukuda, T. Miura, T. Shibata, S. Yamasaki

    Jpn J Infect Dis 雑誌 国立感染症研究所   2015

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  • Cytolethal distending toxin gene-based multiplex PCR assay for Campylobacter jejuni, C. coli, C. fetus, C. upsaliensis, C. hyointestinalis and C. lari Reviewed

    K. Kamei, H. Kawabata, M. Asakura, W. Samosornsuk, A. Hinenoya, S. Nakagawa, S. Yamasaki

    Jpn J Infect Dis 雑誌 国立感染症研究所   2015

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  • Development of a PCR-restriction fragment length polymorphism assay for detection and subtyping of cholix toxin variant genes of Vibiro cholerae Reviewed

    S.P. Awasthi, M. Asakura, S.B. Neogi, A. Hinenoya, T. Ramamurthy, S. Yamasaki

    J. Med. Microgiol. Society for General Microbiology   63 ( 5 )   667 - 673   2014.05

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  • Development of a PCR-restriction fragment length polymorphism assay for detection and subtyping of cholix toxin variant genes of Vibrio cholerae Reviewed

    S.P. Awasthi, M. Asakura, S.B. Neogi, A. Hinenoya, T. Ramamurthy, S. Yamasaki

    J Med Microbiol 雑誌 Society for General Microbiology   63 ( 5 )   667 - 673   2014.05

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  • A PCR-RFLP assay for the detection and differentiation of Campylobacter jejuni, C. coli, C. fetus, C. hyointestinalis, C. lari, C. helveticus and C. upsaliensis Reviewed

    K. Kamei, M. Asakura, S. Somroop, N. Hatanaka, A. Hinenoya, A. Nagita, N. Misawa, M. Matsuda, S. Nakagawa, S. Yamasaki

    J. Med. Microgiol. Society for General Microbiology   63 ( 5 )   659 - 666   2014.05

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  • Molecular characterization of cytolethal distending toxin gene-positive Escherichia coli from healthy cattle and swine in Nara, Japan Reviewed

    A. Hinenoya, K. Shima, M. Asakura, K. Nishimura, T. Tsukamoto, T. Ooka, T. Hayashi, T. Ramamurthy, S.M. Faruque, S. Yamasaki

    BMC Microbiol. BioMed Central   doi: 10.1186/1471-2180-14-97.   2014.04

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  • Molecular characterization of cytolethal distending toxin gene-positive Escherichia coli from healthy cattle and swine in Nara, Japan Reviewed

    A. Hinenoya, K. Shima, M. Asakura, K. Nishimura, T. Tsukamoto, T. Ooka, T. Hayashi, T. Ramamurthy, S.M. Faruque, S. Yamasaki

    BMC Microbiol 雑誌 BioMed Central Ltd.   14   2014.04

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  • Prevalence of Vibrio cholerae O1 El Tor variant in a cholera-epidemic zone of Kenya Reviewed

    S.M. Saidi, N. Chowdhury, S.P. Awasthi, M. Asakura, A. Hinenoya, Y. Iijima, S. Yamasaki

    J. Med. Microgiol. Society for General Microbiology   63 ( 3 )   415 - 420   2014.03

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  • Novel cholix toxin variants, ADP-ribosylating in Vibrio cholerae non-O1/non-O139 strains, and their pathogenicity Reviewed

    S.P. Awasthi, M. Asakura, N. Chowdhury, S.B. Neogi, A. Hinenoya, H.M. Golbar, J. Yamate, E. Arakawa, T. Tada, T. Ramamurthy, S. Yamasaki

    Infect. Immun. American Society for Microbiology   81 ( 2 )   531 - 541   2013.02

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  • Molecular characterizations of cytolethal distending toxin produced by Providencia alcalifaciens strains isolated from patients with diarrhea Reviewed

    A. Shima, A. Hinenoya, M. Asakura, N. Sugimoto, T. Tsukamoto, H. Ito, A. Nagita, S.M. Faruque and S. Yamasaki

    Infect. Immun. American Society for Microbiology   80 ( 4 )   1323 - 1332   2012.04

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  • Prevalence of Providencia strains among children with diarrhea in Japan Reviewed

    A. Shima, A. Hinenoya, M. Asakura, A. Nagita, S. Yamasaki

    Jpn. J. Infect. Dis. National Institute of Infectious Diseases   65 ( 6 )   545 - 547   2012

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  • Evaluation of a PCR-restriction fragment length polymorphism (PCR-RFLP) assay for molecular epidemiological study of Shiga toxin-producing Escherichia coli Reviewed

    N. Sugimoto, K. Shima, A. Hinenoya, M. Asakura, A. Matsuhisa, H. Watanabe and S. Yamasaki

    J. Vet. Med. Sci. Japanese Society of Veterinary Science   73 ( 7 )   859 - 867   2011.07

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  • Inhibition of virulence potential of Vibrio cholerae by natural compounds

    S. Yamasaki, M. Asakura, S. B. Neogi, A. Hinenoya, E. Iwaoka and S. Aoki

    Indian J. Med. Res. Indian Journal of Medical Research   133   232 - 239   2011.02

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  • Identification of Vibrio campbellii isolated from diseased farm-shrimps from south India and establishment of its pathogenic potential in an Artemia model. Reviewed

    S. Haldar, S. Chatterjee, N. Sugimoto, S. Das, N. Chowdhury, A. Hinenoya, M. Asakura and S. Yamasaki

    Microbiology Society for General Microbiology   157 ( 1 )   179 - 188   2011.01

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  • Evaluation of a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the identification of Campylobacter strains isolated from diarrheal patients in Japan Reviewed

    S. M. Kabir, K. Kikuchi, M. Asakura, S. Shiramaru, N. Tsuruoka, A. Goto, A. Hinenoya and S. Yamasaki

    Jpn. J. Infect. Dis. National Institute of Infectious Diseases   64 ( 1 )   19 - 27   2011.01

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  • Candida albicans, Cryptococcus neoformans or Aspergillus fumigatus induces an antifungal activity in mouse serum, which is different from transferrin Reviewed

    K. Okazaki, M. Asakura, N. Sugimoto, A. Hinenoya and S. Yamasaki

    J. Vet. Med. Sci. Japanese Society of Veterinary Science   71 ( 11 )   1459 - 1464   2010.11

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  • Identification of Vibrio harveyi as a causative bacterium for tail rot disease of sea bream Sparus aurata from research hatchery in Malta. Reviewed

    S. Haldar, A. Maharajan, S. Chatterjee, S.A. Hunter, N. Chowdhury, A. Hinenoya, M. Asakura and S. Yamasaki

    Microbiol. Res. ELSEVIER   165 ( 8 )   639 - 648   2010.10

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  • Molecular epidemiology of Vibrio cholerae and Campylobacter isolated in Asian countries.

    S. Yamasaki , M.Asakura , S. Shiramaru, S.B. Neogi, A. Hinenoya, W. Samosornsuk, L. Shi and T. Ramamurthy

    Current Topics of Infectious Diseases in Japan and Asia Springer   1   25 - 43   2010.10

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  • A highly sensitive and specific multiplex PCR assay for simultaneous detection of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus. Reviewed

    S.B. Neogi, N. Chowdhury, M. Asakura, A. Hinenoya, S. Haldar, S.M. Saidi, K. Kogure, R.J. Lara and S. Yamasaki

    Lett. Appl. Microbiol. Wiley-Blackwell   51 ( 3 )   293 - 300   2010.09

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  • Development of simple and rapid PCR-fingerprinting methods for Vibrio cholerae on the basis of genetic diversity of the superintegron. Reviewed

    N. Chowdhury, M. Asakura, S.B. Neogi, A. Hinenoya, S. Haldar, T. Ramamurthy, B.L. Sarkar, S.M. Faruque and S. Yamasaki

    J. Appl. Microbiol. Wiley-Blackwell   109 ( 1 )   304 - 312   2010.07

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  • Distribution of virulence genes related to adhesions and toxins in Shiga toxin-producing Escherichia coli strains isolated from healthy cattle and diarrheal patients in Japan Reviewed

    Y. Wu, A. Hinenoya, T. Taguchi, A. Nagita, K. Shima, T. Tsukamoto, N. Sugimoto, M. Asakura and S. Yamasaki

    J. Vet. Med. Sci. Japanese Society of Veterinary Science   72 ( 5 )   589 - 597   2010.05

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  • Capsaicin, a potential inhibitor of cholera toxin production in Vibrio cholerae. Reviewed

    S. Chatterjee, M. Asakura, N. Chowdhury, S.B. Neogi, N. Sugimoto, S. Haldar, S.P. Awasthi, A. Hinenoya, S. Aoki and S. Yamasaki

    FEMS Microbiol. Lett.   306 ( 1 )   54 - 60   2010.05

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  • Development of a haemolysin gene-based multiplex PCR for simultaneous detection of Vibrio campbellii, Vibrio harveyi and Vibrio parahamolyticus Reviewed

    S. Haldar, S.B. Neogi, K. Kogure, S. Chatterjee, N. Chowdhury, A. Hinenoya, M. Asakura and S. Yamasaki

    Lett. Appl. Microbiol. Wiley-Blackwell   50 ( 2 )   146 - 152   2010.02

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  • Prevalence and characteristics of cytolethal distending toxin (Cdt)-producing Escherichia coli from children with diarrhea in Japan Reviewed

    A. Hinenoya, A. Naigita, K. Ninomiya, M. Okuda, K. Shima, M. Asakura, K. Nishimura, K. Seto, T. Tsukamoto, T. Ramamurthy and S. Yamasaki

    Microbiol. Immunol. Wiley-Blackwell   53 ( 4 )   206 - 215   2009.04

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  • Rapid culture-free identification and molecular typing of Shiga toxin-producing Escherichia coli by PCR-RFLP Reviewed

    K. Shima, N. Kawamura, A. Hinenoya, N. Sugimoto, Y. Wu, M. Asakura, K. Nishimura, G.B. Nair and S. Yamasaki

    Microbiol. Immunol. Wiley-Blackwell   52 ( 6 )   310 - 313   2008.06

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  • Development of a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the detection and identification of Campylobacter jejuni, Campylobacter coli and Campylobacter fetus Reviewed

    M. Asakura, W. Samosornsuk, A. Hinenoya, N. Misawa, K. Nishimura, A. Matsuhisa and S. Yamasaki

    FEMS Immunol. Med. Microbiol. Federation of European Microbiological Societies   52 ( 2 )   260 - 266   2008.03

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    Kind of work：Joint Work  

  • An inducible lambdoid prophage encoding cytolethal distending toxin (Cdt-I) and a type III effector protein in enteropathogenic Escherichia coli Reviewed

    M. Asakura, A. Hinenoya, M. S. Alam, K. Shima, S. H. Zahid, L. Shi, N. Sugimoto, A. N. Ghosh, T. Ramamurthy, S. M. Faruque, G. B. Nair and S. Yamasaki

    Proc. Natl. Acad. Sci. USA National Academy of Sciences   104 ( 36 )   14483 - 14488   2007.09

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    Kind of work：Joint Work  

  • Cytolethal distending toxin (Cdt)-producing Escherichia coli isolated from a child with bloody diarrhea in Japan Reviewed

    A. Hinenoya, A. Nagita, M. Asakura, T. Tsukamoto, T. Ramamurthy, G. B. Nair, Y. Takeda and S. Yamasaki.

    Microbiol. Immunol. Wiley-Blackwell   51 ( 4 )   435 - 438   2007.04

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    Kind of work：Single Work  

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Books and Other Publications

  • ベトナムの養鶏場における薬剤耐性菌の現状

    日根野谷淳

    日本防菌防黴学会  2018.12 

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    Responsible for pages：551-560  

  • 消化管感染症の発症メカニズム（下痢原性大腸菌）

    山崎伸二, 日根野谷淳（ Role： Joint author）

    化学療法の領域  2016.09 

  • カンピロバクター感染症

    山崎伸二、日根野谷淳（ Role： Joint author）

    医学書院  2014.07 

  • Inhibition of virulence potential of Vibrio cholerae by natural compounds

    S Yamasaki, M. Asakura, S.B. Neogi, A Hinenoya, E Iwaoka, S Aoki（ Role： Joint author）

    Indian J Med Res  2011.02 

  • Molecular epidemiology of Vibrio cholerae and campylobacters isolated in Asian countries

    S Yamasaki, M Asakura, S Shiramaru, A Hinenoya, W Samosornsuk, L Shi, T Ramamurthy（ Role： Sole author）

    Springer  2010.02 

MISC

  • 新興人獣共通感染症細菌Escherichia albertiiに関する研究

    日根野谷 淳

    日本細菌学雑誌   76 ( 4 )   175 - 185   2021.11（ ISSN:0021-4930 ）

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    Escherichia albertiiは2003年に新たに命名された新興人獣共通腸管感染症細菌である。近年国内では,患者数100名を超える規模の集団食中毒事例が複数報告されている。本菌は腸管病原性大腸菌と同様,付着因子インチミンをコードするeae遺伝子を保有するが,一部の菌株が腸管出血性大腸菌(EHEC)の主要病原因子である志賀毒素2(stx2a,stx2f)遺伝子を保有することも明らかになったことから,注意を要する細菌であると言える。しかしながら,本菌の基本性状についての情報は乏しく,検査法も確立されていなかったため,本菌感染症の発生状況,感染源,感染経路など未だ不明な点が多い。著者は,継続的に行ってきた細胞膨化致死毒素産生大腸菌の分子疫学研究の中での偶然の発見がきっかけで本菌の研究を始めることとなった。これまでの研究では,本菌の検出,分離,同定を高精度に行える方法を構築し,この方法を用いて本菌の自然宿主の同定を目的とした保菌動物の調査を行ってきた。本稿では,これらの成果について紹介したい。(著者抄録)

  • [Molecular epidemiology of Escherichia albertii, emerging zoonotic enteropathogen]. International journal

    Atsushi Hinenoya

    Nihon saikingaku zasshi. Japanese journal of bacteriology   76 ( 4 )   175 - 185   2021（ ISSN:0021-4930 ）

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    Escherichia albertii is an emerging zoonotic enteric pathogen, closely related to E. coli. Several foodborne outbreaks caused by E. albertii accounting for >100 patients have recently occurred in Japan. This bacterium carries eae gene, similar to enteropathogenic E. coli. Some of them harbor Shiga toxin 2 (stx2a, stx2f) genes, primary virulence factor of enterohemorrhagic E. coli (EHEC), suggesting that the Stx2 producers could cause severe diseases such as HUS in humans. However, due to lack of the knowledges about its bacteriological characteristics and of the diagnostic methods, E. albertii-related infections might have been underestimated, and the infection sources and routes have not yet been understood. We had continuously performed molecular epidemiological studies targeting for cytolethal distending toxin-producing E. coli, and unexpectedly found that cdt-II gene-positive isolates were not E. coli but E. albertii. This finding led us to initiate research more focusing on E. albertii. We have constructed simple, efficient and reliable methods for the detection, isolation and identification of this bacterium by developing an E. albertii-specific PCR assay targeting Eacdt genes and E. albertii-selective isolation medium named XRM-MacConkey agar. We have also identified raccoons as a potential natural reservoir of E. albertii through wildlife survey using these methods. Here, I describe what I have studied with my colleagues.

    DOI： 10.3412/jsb.76.175

    PubMed

Presentations

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Industrial Property Rights

  • 選択分離用培地

    山崎伸二、日根野谷淳

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    property_type：Patent 

  • 消毒された肝臓の製造方法

    山崎伸二、日根野谷淳、森河内巌、山口葵、櫻本行利、西田和正、朝倉昌博

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    property_type：Patent 

    Patent/Registration no：6087915 

Grant-in-Aid for Scientific Research

  • 新興人獣共通感染症細菌Escherichia albertiiの環境内動態における水環境の重要性評価

    Grant-in-Aid for Scientific Research(C)  2026

  • 新興食中毒細菌Escherichia albertiiにおける新規分散付着因子の同定と性状解析

    Grant-in-Aid for Scientific Research(C)  2026

  • 新興人獣共通感染症細菌Escherichia albertiiの環境内動態における水環境の重要性評価

    Grant-in-Aid for Scientific Research(C)  2025

  • 新興食中毒細菌Escherichia albertiiにおける新規分散付着因子の同定と性状解析

    Grant-in-Aid for Scientific Research(C)  2025

  • 新興人獣共通感染症細菌Escherichia albertiiの環境内動態における水環境の重要性評価

    Grant-in-Aid for Scientific Research(C)  2024

  • 新興食中毒細菌Escherichia albertiiにおける新規分散付着因子の同定と性状解析

    Grant-in-Aid for Scientific Research(C)  2024

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Charge of on-campus class subject

  • 食品衛生検査学実習

    2024   Weekly class   Undergraduate

  • 獣医伝染病学B

    2024   Weekly class   Undergraduate

  • 獣医伝染病学A

    2024   Weekly class   Undergraduate

  • 人獣共通感染症学

    2024   Weekly class   Undergraduate

  • 獣医細菌学

    2024   Intensive lecture   Undergraduate

  • 獣医細菌学

    2024   Weekly class   Undergraduate

  • 小動物基礎臨床実習

    2024   Intensive lecture   Undergraduate

  • 獣医微生物・免疫学実習

    2024   Weekly class   Undergraduate

  • 獣医伝染病学B

    2024   Weekly class   Undergraduate

  • 獣医感染制御学

    2024   Weekly class   Undergraduate

  • 獣医科学英語演習

    2024   Intensive lecture   Undergraduate

  • Special Lecture: Infectious Diseases Control C

    2021    

  • Exercise in International Food Circulation

    2021   Practical Training  

  • Practice in Food Hygiene

    2021   Practical Training  

  • Practice in Veterinary Infectious Disease Control

    2021   Practical Training  

  • Practice in Analysis on Food Sanitation and Hygiene

    2021   Practical Training  

  • Topics in Life, Environment, and Advanced Sciences

    2021    

  • Infectious Diseases of Animals B

    2021    

  • Practice in Veterinary Microbiology and Immunology

    2021   Practical Training  

  • Veterinary Bacteriology

    2021    

  • Basic clinical practices

    2021   Practical Training  

  • Veterinary Infectious Disease Control

    2021    

  • Veterinary International Prevention of Epidemics

    2021    

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