Updated on 2025/04/08

写真a

 
Hinenoya Atsushi
 
Organization
Graduate School of Veterinary Science Department of Veterinary Science Associate Professor
School of Veterinary Science Department of Veterinary Science
Title
Associate Professor
Affiliation
Institute of Veterinary Science

Position

  • Graduate School of Veterinary Science Department of Veterinary Science 

    Associate Professor  2022.04 - Now

  • School of Veterinary Science Department of Veterinary Science 

    Associate Professor  2022.04 - Now

Degree

  • Doctor (Veterinary Sciences) （ Others ） (   Osaka Prefecture University )

Research Areas

  • Life Science / Bacteriology  / Bacteriology

  • Life Science / Bacteriology

Research subject summary

  • Escherichia albertiiの病原性および分子疫学研究

  • 志賀毒素産生性大腸菌の病原性に関する研究

  • 大腸菌が産生する細胞膨化致死毒素の病原性に関する研究

Research Career

  • Approach to virulence mechanism of Escherichia albertii

    Individual

    2015.04 - Now 

  • Molecular epidemiology of Escherichia albertii

    International Joint Research

    2014.04 - Now 

  • Analysis of pathogenecity of Shiga toxin-producing Escherichia coli

    Shiga toxin, STX, Escherichia coli, diarrhea  Individual

  • Molecular epidemiology of Cytolethal distending toxin-producing Escherichia coli

    Cytolethal distending toxin, CDT, Escherichia coli, diarrhea  Individual

Professional Memberships

  • Japan Veterinary Medical Association

    2020.10 - Now

  • 日本食品微生物学会

    2015.01 - Now   Domestic

  • The Japanese Association for Infectious Diseases

    2011 - 2019   Domestic

  • 腸管出血性大腸菌感染症研究会

  • TOXIN SYMPOSIUM

  • AMERICAN SOCIETY FOR MICROBIOLOGY

  • THE JAPANESE ASSOCIATION FOR INFECTIOUS DISEASES

  • THE JAPANESE SOCIETY OF VETERINARY SCIENCE

  • JAPANESE SOCIETY FOR BACTERIOLOGY

  • JAPANESE SOCIETY OF FOOD MICROBIOLOGY

  • Enterohaemorrhagic Escherichia coli symposium

      Domestic

  • Japanese Society of Veterinary Science

      Domestic

  • Toxin Synposium

      Domestic

  • American Society for Microbiology

      Overseas

  • Japanese Society for Microbiology

      Domestic

▼display all

Committee Memberships (off-campus)

  • councilor   The Japanese Society for Food Microbiology  

    2023.01 - Now 

Awards

  • 黒屋奨学賞

    2021.03   Japanese Society for Bacteriology   Research on an emerging zoonotic pathogen Escherichia albertii

  • 研究奨励賞

    2016.11   腸管出血性大腸菌感染症研究会  

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    Country：Japan

  • 研究奨励賞

    2011.07   腸管出血性大腸菌感染症研究会  

    　More details

    Country：Japan

Job Career (off-campus)

  • Osaka Metropolitan University   Graduate School of Veterinary Science

    2022.04 - Now

  • University of Tennessee   College of Animal Science   Visiting Scholar

    2017.10 - 2019.02

  • Osaka Prefecture University   Department of Veterinary Science, Graduate School of Life and Environmental Sciences

    2017.04 - 2022.03

Papers

  • Occurrence and cross contamination of Escherichia albertii in retail chicken outlets in Bangladesh.

    Jayedul Hassan, Kishor Sosmith Utsho, Susmita Karmakar, Md Wohab Ali, Sharda Prasad Awasthi, Chiharu Uyama, Noritoshi Hatanaka, Shinji Yamasaki, Atsushi Hinenoya

    International journal of food microbiology   431   111081 - 111081   2025.03（ ISSN:01681605 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Escherichia albertii is an emerging zoonotic pathogen linked to human gastrointestinal illnesses, with poultry meats being considered as a key source of human infections. However, there is little information regarding the distribution and characteristics of this bacterium in Bangladesh. This study investigated the occurrence, antimicrobial resistance, and virulence of E. albertii in chicken meats from retail outlets in Bangladesh. We collected samples from 61 dressed chickens across 17 retail shops from 4 upazilas, along with swabs from cloaca, processing utensils, and worker hands. Detection of E. albertii by species-specific PCR revealed substantial occurrence of E. albertii in retail chicken meat (63.9 %), cloaca (71.4 %), human hand (45.5 %), bleeding cone (13.3 %) and blade (10 %). Almost all the E. albertii isolates (94.4 %) exhibited resistance to at least one of the tested antimicrobials, among which 50 % were multidrug resistant, including resistance to clinically relevant antimicrobials such as tetracycline, ampicillin, gentamicin, kanamycin, nalidixic acid and ciprofloxacin. Whole genome sequencing analysis identified the presence of corresponding antimicrobial resistance genes and critical virulence genes (eae, Eacdt). Notably, although wgSNP-based phylogenetic analysis showed the genomic diversity of the isolates, some of the isolates from the same shop displayed clonal relationships among meats, cloacal swabs, and human hand swabs, indicating contamination during processing. These findings highlight the public health risk posed by E. albertii in retail poultry, underlining the poultry's role as a potential vector for zoonotic transmission and the need for improved biosecurity and antimicrobial management practices in poultry production.

    DOI： 10.1016/j.ijfoodmicro.2025.111081

    PubMed

  • Prevalence of potentially pathogenic and antimicrobial-resistant Escherichia coli in raw milk and dairy products in Egypt

    Asmaa M. Elbastawesy, Sharda Prasad Awasthi, Noritoshi Hatanaka, Atsushi Hinenoya, Atsushi Iguchi, Rabee A. Ombarak, Azza M.M. Deeb, Shinji Yamasaki

    International Dairy Journal   162   2025.03（ ISSN:0958-6946 ）

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    Publishing type：Research paper (scientific journal)  

    Milk and dairy products are popular in Egyptian diets, but their contamination with Escherichia coli, poses health risks. This study investigated the prevalence of potentially pathogenic and antimicrobial-resistant E. coli in raw milk and dairy products from Kafrelsheikh and Algarbia Governorates, Egypt. Two hundred ten samples including raw buffalo milk, goat milk, Domiati cheese, Domiati cheese with pepper, rayeb, and yogurt were analyzed. The prevalence of E. coli was 26.2%, with the highest occurrence in buffalo milk (68.0%) and the lowest in rayeb (7.5%). Based on ERIC-PCR, eighty-four non-clonal E. coli strains were selected and further characterized. Among tested virulence genes, adhesion genes such as lpfAO113 and ehaA, were the most prevalent. Toxin-encoding genes such as astA, cdt, cnf, and hlyA were also detected. The cytotoxic and hemolytic activity of cdt, cnf, and hylA carrying E. coli were confirmed on CHO cells and sheep blood agar, respectively. Twenty-three (27.4%) strains showed resistance to one or more antimicrobials, and 10 (11.9%) strains exhibited multidrug resistance (MDR). Among 12 antimicrobials tested resistance against ampicillin, streptomycin and tetracycline was the highest. Phylogenetic analysis and O-genotyping indicated clinically significant strains such as Og103, Og157 and OgGp9. Notably, two OgGp9 strains were OgGp9:Hg18 and phylogenetic group D, like those associated with a large diarrheal outbreak caused by milk consumption in Japan, in 2021. Interestingly, these two strains harbored a complete type 3 secretion system 2 locus (ETT2) and one of these strains was MDR. These findings indicate that these dairy products were contaminated with potentially pathogenic and multidrug-resistant E. coli. This is the first report to analyze E. coli contamination in Domiati cheese with pepper and detect OgGp9:Hg18 outbreak-associated strains with ETT2 and MDR in Egypt.

    DOI： 10.1016/j.idairyj.2024.106145

  • Wild raccoons (Procyon lotor) as a potential reservoir of cytolethal distending toxin-producing Providencia strains in Japan.

    Okechukwu John Obi, Atsushi Hinenoya, Sharda Prasad Awasthi, Noritoshi Hatanaka, Shah M Faruque, Shinji Yamasaki

    Microbiology spectrum   e0261624   2025.02

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    In view of increasing reports of infections due to virulent Providencia species including cytolethal distending toxin (cdt) gene-positive strains, it is important to identify the reservoirs and transmission routes of such pathogenic strains. Raccoons considered to be a source of zoonotic pathogens were monitored for the presence of Providencia species in Japan and analyzed for cdt genes. Of 384 wild raccoon rectal swabs analyzed, 60% were positive for Providencia species, of which 20% carried cdt-genes. Among seven Providencia species isolated (P. alcalifaciens, P. rustigianii, P. rettgeri, P. stuartii, P. heimbachae, P. vermicola, and P. huaxiensis), cdt genes were distributed in P. alcalifcaiens (63%), P. rustigianii (16%), and novel in P. rettgeri (21%). Complete cdt gene clusters were identified in P. alcalifaciens and P. rustigianii strains, whereas P. rettgeri had intact cdtB but truncated cdtA and cdtC genes. Phylogenetic analyses showed divergent pulsotypes among the cdt gene-positive Providencia strains. Cytotoxicity assay revealed that P. alcalifaciens and P. rustigianii produced CDT more toxic to eukaryotic cells compared to human clinical strains, which were neutralized by anti-PaCdtB serum. As expected, the P. rettgeri strains with truncated cdt genes had no biological activity. Molecular analysis revealed that all the cdt genes were located on plasmids as determined by S1-nuclease pulsed-field gel electrophoresis (S1-PFGE) and Southern hybridization assay. Intriguingly, the cdtB gene in P. rustigianii strains was detected on dual plasmids. Notably, all the cdt gene-positive Providencia strains were found to carry plasmid-mediated T3SS-related genes. These results suggest that wild raccoons are possible reservoir of virulent Providencia strains in Japan.IMPORTANCEProvidencia species considered normal flora are occasionally associated with gastroenteritis in healthy humans. Cytolethal distending toxin (CDT), a bacterial virulence factor found in various Gram-negative bacteria and associated with gastroenteritis and extra-intestinal infection has also been reported in at least two Providencia species (P. alcalifaciens and P. rustigianii). Determination of the transmission routes of such virulent Providencia is crucial for the implementation of evidence-based control programs. In this study, we identified raccoons as the probable reservoir of the cdt gene-positive Providencia strains in Japan. Interestingly, CDTs produced by raccoon-derived Providencia strains exerted more toxic effects on the eukaryotic cells compared to the clinical Providencia strains. In addition, the identification of a novel cdt gene cluster in another species P. rettgeri isolated from raccoons suggests that Providencia may be categorized as an emerging zoonotic pathogen.

    DOI： 10.1128/spectrum.02616-24

    PubMed

  • Development of a novel modified selective medium cefixime-tellurite-phosphate-xylose-rhamnose MacConkey agar for isolation of Escherichia albertii and its evaluation with food samples.

    Keiji Takehira, Goutham Belagula Manjunath, Noritoshi Hatanaka, Sharda Prasad Awasthi, Bingting Xu, Akira Nagita, Rupak K Bhadra, Atsushi Hinenoya, Shinji Yamasaki

    International journal of food microbiology   430   111057 - 111057   2025.02（ ISSN:01681605 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Since cefixime and tellurite are known to inhibit most bacteria belonging to Enterobacterales, we found that addition of tellurite inhibited E. albertii growth in Luria Bertani broth but not in tryptic soy broth (TSB), and addition of phosphate and soy peptone enhanced E. albertii growth in TSB in presence of tellurite. Subsequently, to find the positive factor present in TSB, E. albertii growth was examined in tryptone, soy peptone, glucose, or phosphate deficient tryptic soy agar plates. Phosphate, soy peptone, and/or glucose deficiency indeed decreased E. albertii growth. However, none of the substances are specifically present in xylose-rhamnose-melibiose (XRM)-MacConkey agar and thus, not affecting E. albertii growth. Altogether, a novel E. albertii selective differential medium, XRM-MacConkey medium containing cefixime (C), tellurite (T), phosphate (P), and soy peptone (S) (named CT-PS-XR-MacConkey medium), which differentiate E. albertii (colorless) from E. coli (red) by colony color, has been developed. The CT-PS-XR-MacConkey agar was evaluated with 156 bacterial strains including 65 E. albertii. While all E. albertii strains grew as colorless colonies, 54 strains of 9 different genera belonging to 19 different species were unable to grow on this medium. However, rest of these bacterial strains grew either as colorless or as red colonies. Furthermore, spiking experiments using chicken meat as food samples showed that the CT-PS-XR-MacConkey medium is highly selective for E. albertii than XRM-MacConkey agar. Altogether, the results suggest that the CT-PS-XR-MacConkey agar is indeed a useful selective medium for isolation of E. albertii from food samples.

    DOI： 10.1016/j.ijfoodmicro.2025.111057

    PubMed

  • Morphological identification and phylogenetic analysis of Eimeria coypi and Eimeria fluviatilis (Apicomplexa: Eimeriidae) isolated from nutrias (Myocastor coypus [Rodentia]) in Japan. Reviewed

    Sora Ouchi, Ryosuke Koda, Yuzuru Ishizuka, Shigetoyo Ikemoto, Mutsuko Sakata, Susumu Iwaide, Tomoyuki Shibahara, Atsushi Hinenoya, Shigehiko Uni, Kazumi Sasai, Makoto Matsubayashi

    Systematic parasitology   102 ( 1 )   18 - 18   2025.01（ ISSN:01655752 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    The nutria (Myocastor coypus) is a semiaquatic rodent that originally inhabited South America. However, the animals have spread to different continents as alien species, and their numbers are quickly increasing, especially in North America, Europe, and Eastern Asia including Japan. Although nutrias have been suggested to serve as reservoirs for pathogens, including parasites, there have been few reports on this subject. In the present study, we surveyed the gastrointestinal parasites in nutrias living in Japan to better understand their prevalence in nutrias. We collected 72 samples of intestinal contents or feces from nutrias in Osaka and Okayama Prefectures. We found that 49 (68.1 %) samples were positive for Eimeria parasites, and two types of oocysts were identified: ellipsoidal (Type A) and subspherical (Type B) oocysts. In addition, Strongyloides myopotami was detected in 44 samples, and Capillaria spp. and Fasciola spp. were detected in one and three samples, respectively. Based on the morphologies of the detected Eimeria oocysts, Types A and B were identified to be E. coypi and E. fluviatilis, respectively. Phylogenetic analyses after PCR and sequencing targeting the cytochrome c oxidase subunit I gene placed the sequences of E. fluviatilis (Type B) as a cluster between the sequences of Eimeria derived from rodents. The sequences of the three subgenotypes of E. coypi (Type A) were included in the cluster containing the sequences of Eimeria spp. from rodents of multiple species, which is referred to as the Apionodes supercluster, and is separate from other clades. It has been suggested that Eimeria spp. from rodents in this cluster can quickly adapt to infect different hosts. Although further analyses are needed to construct more detailed phylogenetic trees, our results revealed the genetical positions of Eimeria spp. in nutrias. In addition, our results may be helpful when considering host specificity as well as host switching by the pathogen.

    DOI： 10.1007/s11230-025-10216-0

    PubMed

  • Evaluation of a novel modified selective medium cefixime-tellurite-phosphate-xylose-rhamnose MacConkey agar for the isolation of <i>Escherichia albertii</i> from diarrheal stool specimens

    TAKEHIRA Keiji, AWASTHI Sharda Prasad, HATANAKA Noritoshi, NAGITA Akira, HINENOYA Atsushi, YAMASAKI Shinji

    Journal of Veterinary Medical Science   87 ( 3 )   308 - 314   2025（ ISSN:09167250 ） （ eISSN:13477439 ）

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    <p>It is challenging to isolate <i>Escherichia albertii</i> from clinical specimens. Therefore, a medium that can selectively grow <i>E. albertii</i> and differentiate it from <i>E. coli</i> is earnestly desired. Here, we describe the evaluation of a recently developed selective differential medium, called cefixime-tellurite-phosphate-xylose-rhamnose-MacConkey (CT-PS-XR-MacConkey) medium, which enables the specific growth of <i>E. albertii</i> and differentiation of <i>E. albertii</i> (colorless) from <i>E. coli</i> (red) based on colony color and thus, facilitating the efficient isolation of <i>E. albertii</i> from diarrheal stool. When three <i>E. albertii</i> negative diarrheal stools were inoculated onto CT-PS-XR-MacConkey and xylose-rhamnose-melibiose (XRM) containing MacConkey agars, no colorless colonies were observed on both the media. However, when <i>E. albertii</i> was spiked into these three diarrheal stools, the ratio of colorless colonies to red colonies was higher on CT-PS-XR-MacConkey agar compared to XRM-MacConkey agar in all three samples. Notably, out of 105 <i>Eacdt</i>-gene PCR negative diarrheal stools 56 yielded colorless colonies on MacConkey agar while out of these 56 diarrheal stools, nine yielded colorless colonies on XRM-MacConkey but no colorless colonies were observed on CT-PS-XR-MacConkey agar. Furthermore, evaluation of these two media with five <i>E. albertii</i> positive-stool specimens revealed that the number of red colonies were constantly less, whereas that of colorless colonies were constantly more on CT-PS-XR-MacConkey agar, thus aiding in efficient isolation. Altogether, these results suggest that the CT-PS-XR-MacConkey agar could be a useful selective differential medium for isolation of <i>E. albertii</i> from diarrheal stool specimens.</p>

    DOI： 10.1292/jvms.24-0500

    PubMed

  • A plasmid-mediated type III secretion system associated with invasiveness and diarrheagenicity of Providencia rustigianii.

    Jayedul Hassan, Atsushi Hinenoya, Noritoshi Hatanaka, Sharda Prasad Awasthi, Goutham Belagula Manjunath, Nahid Rahman, Jyoji Yamate, Shota Nakamura, Daisuke Motooka, Akira Nagita, Shah M Faruque, Shinji Yamasaki

    mBio   15 ( 10 )   e0229724   2024.09（ ISSN:21612129 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    We have recently described a clinical isolate of Providencia rustigianii strain JH-1 carrying the genes for cytolethal distending toxin (CDT) in a conjugative plasmid. A cdtB mutant of strain JH-1, which lost CDT activity, was still found to retain invasiveness and diarrheagenicity. The strain was subjected to phenotypic and genetic analyses including whole genome sequencing (WGS) to explore the genetic determinants of the observed invasiveness and diarrheagenic properties. Analysis and annotation of WGS data revealed the presence of two distinct type III secretion systems (T3SS) in strain JH-1, one of which was located on the chromosome designated as cT3SS (3,992,833 bp) and the other on a mega-plasmid designated as pT3SS (168,819 bp). Comparative genomic analysis revealed that cT3SS is generally conserved in Providencia spp. but pT3SS was limited to a subset of Providencia spp., carrying cdt genes. Strain JH-1 was found to invade HeLa cells and induce fluid accumulation with characteristic pathological lesions in rabbit ileal loops. Remarkably, these phenomena were associated with the pT3SS but not cT3SS. The plasmid could be transferred by conjugation from strain JH-1 to other strains of P. rustigianii, Providencia rettgeri, and Escherichia coli with concomitant transfer of these virulence properties. This is the first report of a functional and mobile T3SS in P. rustigianii and its association with invasiveness and diarrheagenicity of this bacterium. These data suggest that P. rustigianii and other CDT-producing Providencia strains might carry T3SS and exert their diarrheagenic effect by exploiting the T3SS nano-machinery.IMPORTANCEThe precise mechanism of virulence of Providencia rustigianii is unclear, although some strains produce cytolethal distending toxin as a putative virulence factor. We have detected the presence of a type III secretion system (T3SS) for the first time on a plasmid in a P. rustigianii strain. Plasmid-mediated T3SS seems to be directly involved in virulence of P. rustigianii and may serve as a means of horizontal transfer of T3SS genes. Our results may have implication in understanding the mechanism of emergence of new pathogenic strains of P. rustigianii.

    DOI： 10.1128/mbio.02297-24

    PubMed

  • QcrC is a potential target for antibody therapy and vaccination to control Campylobacter jejuni infection by suppressing its energy metabolism

    Koji Hosomi, Noritoshi Hatanaka, Atsushi Hinenoya, Jun Adachi, Yoko Tojima, Mari Furuta, Keita Uchiyama, Makiko Morita, Takahiro Nagatake, Azusa Saika, Soichiro Kawai, Ken Yoshii, Saki Kondo, Shinji Yamasaki, Jun Kunisawa

    Frontiers in Microbiology   15   1415893   2024.07（ ISSN:1664-302X ） （ eISSN:1664-302X ）

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    Publishing type：Research paper (scientific journal)  

    Introduction

    Campylobacter spp. are a public health concern, yet there is still no effective vaccine or medicine available.

    Methods

    Here, we developed a Campylobacter jejuni-specific antibody and found that it targeted a menaquinol cytochrome c reductase complex QcrC.

    Results

    The antibody was specifically reactive to multiple C. jejuni strains including clinical isolates from patients with acute enteritis and was found to inhibit the energy metabolism and growth of C. jejuni. Different culture conditions produced different expression levels of QcrC in C. jejuni, and these levels were closely related not only to the energy metabolism of C. jejuni but also its pathogenicity. Furthermore, immunization of mice with recombinant QcrC induced protective immunity against C. jejuni infection.

    Discussion

    Taken together, our present findings highlight a possible antibody- or vaccination-based strategy to prevent or control Campylobacter infection by targeting the QcrC-mediated metabolic pathway.

    DOI： 10.3389/fmicb.2024.1415893

    PubMed

  • Detection of prolong excretion of Escherichia albertii in stool specimens of a 7-year-old child by a newly developed Eacdt gene-based quantitative real-time PCR method and molecular characterization of the isolates.

    Sharda Prasad Awasthi, Akira Nagita, Noritoshi Hatanaka, Jayedul Hassan, Bingting Xu, Atsushi Hinenoya, Shinji Yamasaki

    Heliyon   10 ( 9 )   e30042   2024.05（ ISSN:24058440 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Escherichia albertii is an emerging zoonotic foodborne pathogen. The clinical significance of this bacterium has increasingly been recognized worldwide. However, diagnostic method has not yet been established and its clinical manifestations are not fully understood. Here, we show that an Eacdt gene-based quantitative real-time PCR (qRT-PCR) developed in this study is 100% specific and sensitive when tested with 39 E. albertii and 36 non-E. albertii strains, respectively. Detection limit of the real-time PCR was 10 colony forming unit (CFU) and 1 pg of genomic DNA per PCR tube. When E. albertii was spiked with 4 × 100-106 CFU per mL to stool of healthy person, detection limit was 4.0 × 103 and 4.0 CFU per mL before and after enrichment culture, respectively. Moreover, the qRT-PCR was able to detect E. albertii in five children out of 246 (2%) but none from 142 adults suffering from gastroenteritis. All five E. albertii strains isolated carried eae and paa genes, however, only one strain harbored stx2f genes. Long-term shedding of stx2f gene-positive E. albertii in a child stool could be detected because of the qRT-PCR developed in this study which might have been missed if only conventional PCR and culture methods were employed. Furthermore, E. albertii isolated from siblings with diarrhea showed clonality by PFGE analysis. Taken together, these data suggest that the Eacdt gene-based qRT-PCR developed for the detection of E. albertii is useful and will assist in determining the real burden and clinical manifestation of E. albertii infections.

    DOI： 10.1016/j.heliyon.2024.e30042

    PubMed

  • Improved molecular identification of Strongyloides myopotami in nutrias using fecal samples.

    Yuga Mori, Atsushi Naka, Ryosuke Koda, Yuzuru Ishizuka, Atsushi Hinenoya, Tomoyuki Shibahara, Kazumi Sasai, Makoto Matsubayashi

    The Journal of veterinary medical science   86 ( 3 )   2024.01

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：Domestic journal  

    Strongyloides myopotami is an intestinal nematode parasite of nutrias. Identification of S. myopotami is conducted based on the morphological characteristics of adult worms or cultured larvae. To widely and effectively understand the infection in nutrias, it would be preferable to develop the molecular identification using a few grams of the feces. Here, we attempted to identify S. myopotami using DNA extracted from eggs obtained from fecal samples. Among previously reported primer pairs targeting the 18S rRNA gene of Strongyloides spp., most could not be successful.We newly designed primers that successfully amplified the partial sequences in S. myopotami, resulting in being sequenced. Our simple protocol can be useful in nationwide surveys for clarifying the risk of human infection.

    DOI： 10.1292/jvms.23-0198

    PubMed

  • <i>Campylobacter fetus</i> isolates from both human patients and healthy cattle carry three distinct cytolethal distending toxin (<i>cdt</i>) gene clusters

    WEN Wen, HATANAKA Noritoshi, SOMROOP Srinuan, AWASTHI Sharda Prasad, HINENOYA Atsushi, YAMASAKI Shinji

    Journal of Veterinary Medical Science   86 ( 12 )   1311 - 1318   2024（ ISSN:09167250 ） （ eISSN:13477439 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：Domestic journal  

    <p><i>Campylobacter fetus</i> is a zoonotic pathogen. Although the precise virulence mechanisms have not yet been fully elucidated, cytolethal distending toxin (CDT) is considered as one of the well-characterized virulence factors in <i>Campylobacter</i>. <i>In silico</i> analysis of the genome of <i>C. fetus</i> type strain ATCC27374<sup>T</sup> indicates that there are three <i>cdt</i> gene clusters, <i>Cfcdt-I</i>, <i>Cfcdt-II</i> and <i>Cfcdt-III</i>. However, it is not clear whether these clusters are ubiquitously present in <i>C. fetus</i> and their association with diseases in humans and animals. In this study, we have analyzed the distribution and nucleotide sequences of these <i>cdt</i> gene clusters in 137 <i>C. fetus</i> strains isolated from human patients and healthy cattle. MLST and PFGE were also applied to determine clonal relationship between <i>C. fetus</i> strains isolated from patients and cattle. We found all <i>C. fetus</i> strains carry three <i>Cfcdt</i> gene clusters by colony hybridization assay and the strains belonged to 38 different pulsotypes. Whole genome sequencing of 38 <i>C. fetus</i> strains was carried out to determine the entire <i>cdt</i> gene cluster sequences and their sequence type (ST). Among 38 strains, six STs were identified, and each <i>cdt</i> gene cluster showed high similarity (>99%). Interestingly, some of these <i>Cfcdt</i> genes are more similar to the <i>cdt</i> genes of other <i>Campylobacter</i> species than other <i>Cfcdt</i> gene types. Altogether, the results suggest that three <i>Cfcdt</i> gene clusters are highly conserved in <i>C. fetus</i> and the strains belonging to ST-6 may be more pathogenic to human.</p>

    DOI： 10.1292/jvms.24-0336

    PubMed

  • Cefixime-tellurite-deoxycholate tryptic soy broth (CTD-TSB), a selective enrichment medium, for enhancing isolation of Escherichia albertii from wild raccoon fecal samples.

    Bingting Xu, Noritoshi Hatanaka, Sharda Prasad Awasthi, Keiji Tekehira, Atsushi Hinenoya, Shinji Yamasaki

    Journal of applied microbiology   134 ( 7 )   2023.07（ ISSN:1364-5072 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    AIM: The aim of this study was to develop a selective enrichment broth for efficient isolation of Escherichia albertii. METHODS AND RESULTS: A total of 412 raccoon rectal swabs suspended in PBS (phosphate-buffered saline) were tested by a real-time PCR to quantify the number of E. albertii followed by its isolation. The number of E. albertii in the PBS suspension strongly affected the isolation rate (1.2%-89%), which notably dropped (≤33%) when the number was <4 log10 CFU ml-1. However, enrichment of PBS suspension containing raccoon feces in tryptic soy broth containing cefixime, tellurite, and deoxycholate (CTD-TSB), the selective medium developed in this study, remarkably improved the isolation efficiency (up to 48%) of E. albertii. CONCLUSIONS: CTD-TSB is a useful enrichment culture medium for E. albertii and contributes to increase its isolation rate.

    DOI： 10.1093/jambio/lxad123

    PubMed

  • Presence of Functionally Active Cytolethal Distending Toxin Genes on a Conjugative Plasmid in a Clinical Isolate of Providencia rustigianii.

    Hassan J, Awasthi SP, Hatanaka N, Hoang PH, Nagita A, Hinenoya A, Faruque SM, Yamasaki S

    Infection and immunity   e0012122   2023.05（ ISSN:0019-9567 ）

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  • Critical role of the RpoE stress response pathway in polymyxin resistance of Escherichia coli.

    Zeng X, Hinenoya A, Guan Z, Xu F, Lin J

    The Journal of antimicrobial chemotherapy   78 ( 3 )   732 - 746   2023.01（ ISSN:0305-7453 ）

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  • Endotoxin-Free Stx2B-C-CPE Vaccine and Its Optimized Adjuvant Regimen for Preventing Food Poisoning.

    Hosomi K, Shimoyama A, Hinenoya A, Hatanaka N, Noguchi T, Ebina H, Tojima Y, Furuta M, Kondoh M, Kiyono H, Yamasaki S, Fukase K, Kunisawa J

    Frontiers in bioscience (Landmark edition)   28 ( 1 )   15   2023.01（ ISSN:27686701 ）

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  • Seasonality of detection rate of <i>Escherichia albertii </i>in wild raccoons (<i>Procyon lotor</i>) in Osaka, Japan

    XU Bingting, HATANAKA Noritoshi, AWASTHI Sharda Prasad, HINENOYA Atsushi, YAMASAKI Shinji

    Journal of Veterinary Medical Science   advpub ( 0 )   2023（ ISSN:09167250 ） （ eISSN:13477439 ）

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    <p><i>Escherichia albertii </i>has increasingly been recognized as an important emerging zoonotic enteropathogen. Raccoon is shown to be one of the most vital reservoirs of this pathogen. <i>E. albertii </i>has been detected in 993 (62%) out of 1,606 wild raccoons in Osaka, Japan from 2017 to 2020 by <i>Eacdt</i>-PCR. The detection rate of <i>E. albertii </i>was increased from May to December (winter) and gradually decreased from January to April (spring). Furthermore, we could isolate <i>E. albertii</i> from 30% (196/664) of <i>Eacdt</i>-PCR positive samples and the monthly isolation rate seems to correlate with its detection rate. These data indicate that there is a seasonality regarding the prevalence of <i>E. albertii </i>in wild raccoon being higher in winter and lower in spring. </p>

    DOI： 10.1292/jvms.23-0372

    PubMed

  • 岡山県および県境に生息する野生動物および保護動物から分離したEscherichia albertiiの同定と性状解析(Isolation and characterization of Escherichia albertii from wild and safeguarded animals in Okayama Prefecture and its prefectural borders, Japan)

    Naka Atsushi, Hinenoya Atsushi, Awasthi Sharda Prasad, Yamasaki Shinji

    The Journal of Veterinary Medical Science   84 ( 9 )   1299 - 1306   2022.09（ ISSN:0916-7250 ）

  • Longitudinal surveillance and comparative characterization of Escherichia albertii in wild raccoons in the United States. Reviewed

    Atsushi Hinenoya, Huiwen Wang, Erin M Patrick, Ximin Zeng, Liu Cao, Xing-Ping Li, Rebecca L Lindsey, Barbara Gillespie, Qiang He, Shinji Yamasaki, Jun Lin

    Microbiological research   262   127109 - 127109   2022.07（ ISSN:0944-5013 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Escherichia albertii is an emerging enteric bacterial pathogen causing watery diarrhea, abdominal distension, vomiting and fever in humans. E. albertii has caused many foodborne outbreaks in Japan and was also reported in other countries worldwide. However, the important animal reservoirs of this pathogen are still largely unknown, impeding us to combat this emerging pathogen. Recently, we reported that wild raccoons (Procyon lotor) and broiler chickens are significant reservoirs of E. albertii in Japan and the U.S., respectively. Here, we performed a longitudinal surveillance to monitor prevalence of E. albertii in wild raccoons in the U.S. and conducted comprehensive comparative analyses of the E. albertii of different origins. A total of 289 fecal swab samples were collected from wild raccoons in Tennessee and Kentucky in the U.S. (2018-2020). Approximately 26% (74/289) of the raccoons examined were PCR-positive for E. albertii and eventually 22 E. albertii isolates were obtained. PFGE analysis showed the U.S. raccoon E. albertii were phylogenetically distant even though the corresponding raccoons were captured from a small area. Unlike the high prevalence of multidrug resistance (83%) observed in previous chicken E. albertii survey, antibiotic resistance was rarely observed in all the U.S. raccoon and 22 Japan raccoon strains with only one Japan strain displaying multidrug resistance (2%). Whole genome sequencing of 54 diverse E. albertii strains and subsequent comparative genomics analysis revealed unique clusters that displayed close evolutionary relationships and similar virulence gene profiles among the strains of different origins in terms of geographical locations (e.g., U.S. and Japan) and hosts (raccoon, chicken, swine, and human). Challenge experiment demonstrated raccoon E. albertii strains could successfully colonize in the chicken intestine at 3 and 8 days postinfection. A pilot environmental survey further showed all the four tested water samples from Tennessee river were E. albertii-positive; two different E. albertii strains, isolated from a single water sample, showed close relationships to those of human origin. Together, the findings from this study provide new insights into the ecology, evolution, and pathobiology of E. albertii, and underscore the need to control the emerging E. albertii in a complex ecosystem using One Health approach.

    DOI： 10.1016/j.micres.2022.127109

    PubMed

  • Corrigendum to "Prevalence of mobile colistin resistance (mcr) genes in extended-spectrum β-lactamase-producing Escherichia coli isolated from retail raw foods in Nha Trang, Vietnam" [Int. J. Food Microbiol. 346 (2021) 109164].

    Le PQ, Awasthi SP, Hatanaka N, Hinenoya A, Hassan J, Ombarak RA, Iguchi A, Tran NTT, Dao KVT, Vien MQ, Le HX, Do HT, Yamamoto Y, Yamasaki S

    International journal of food microbiology   370   109540   2022.06（ ISSN:0168-1605 ）

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  • Detection, Isolation, and Molecular Characterization of Escherichia albertii from Wild Birds in West Japan. Reviewed

    Atsushi Hinenoya, Sharda Prasad Awasthi, Noritomo Yasuda, Keigo Nagano, Jayedul Hassan, Keiji Takehira, Noritoshi Hatanaka, Shun Saito, Takashi Watabe, Miki Yoshizawa, Haruna Inoue, Shinji Yamasaki

    Japanese journal of infectious diseases   75 ( 2 )   156 - 163   2022.03（ ISSN:1344-6304 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：Domestic journal  

    Escherichia albertii is an emerging zoonotic foodborne pathogen. Several outbreaks of E. albertii have occurred, particularly in Japan. Although birds have been considered as one of the most important reservoirs of this bacterium, information regarding its prevalence in birds is still scarce. We performed a survey of E. albertii in wild birds in Japan and examined the characteristics of these isolates. E. albertii-specific genes were detected in five cloacal swabs from 156 birds by PCR. Four E. albertii strains were isolated from a swallow with two different E. albertii strains and two pigeons in a flock using XRM-MacConkey agar. These isolates were assigned to biogroup 3, showed no resistance to any tested antimicrobials, and were classified into two EAO-genotypes (EAOg2 and EAOg33) and were untypable. Similar to clinical E. albertii strains, these isolates carried virulence genes, including eae (n = 4), paa (n = 4), Eccdt-I (n = 2), and stx2f (n = 1), as well as Eacdt. Furthermore, stx2f genes in a strain were located on an inducible bacteriophage, which can confer the ability to produce Stx2f in E. coli. In conclusion, Japanese wild birds carried E. albertii at levels similar to the reported prevalence in birds. These isolates may have the potential to cause gastroenteritis in humans.

    DOI： 10.7883/yoken.JJID.2021.355

    PubMed

  • 西日本の野鳥からのEscherichia albertiiの検出、分離、分子的特性評価(Detection, Isolation, and Molecular Characterization of Escherichia albertii from Wild Birds in West Japan)

    Hinenoya Atsushi, Awasthi Sharda Prasad, Yasuda Noritomo, Nagano Keigo, Hassan Jayedul, Takehira Keiji, Hatanaka Noritoshi, Saito Shun, Watabe Takashi, Yoshizawa Miki, Inoue Haruna, Yamasaki Shinji

    Japanese Journal of Infectious Diseases   75 ( 2 )   156 - 163   2022.03（ ISSN:1344-6304 ）

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    大阪市と高知市の野鳥を対象にEscherichia albertii保有状況を調べた。ハトのクロアカスワブ検体129件と13種類の野鳥のクロアカスワブ検体27件を使用した。増菌培養後、Eacdt遺伝子を標的としたPCRでスクリーニングし、陽性検体をXRM-マッコンキー寒天培地またはマッコンキー寒天培地で培養した。その結果、ツバメ2羽(7.4%)、ハト3羽(2.3%)がPCR陽性となり、全体の検出率は3.2%(5/156)であった。培養検査ではツバメ1羽とハト2羽からE.albertiiが分離された。PFGE解析により、ハト由来株は同一クローン株、ツバメ由来株から2つのクローン株が確認された。分離4株は生物グループ3に割り当てられ、供試16種抗菌薬に感受性を示した。O抗原遺伝子型はツバメ由来2株が型別不能とEAOg2、ハト由来2株はEAOg33であった。分離4株はEacdtの他にeaeとpaaを保有していた。さらにツバメ由来1株はstx2f、ハト由来2株はEccdt-I遺伝子を保有していた。4株のインチミンサブタイプは多様であったが、LEE病原性アイランドの統合部位はphe Uのみであった。マイトマイシンC存在下、プラークアッセイによる溶原化ファージ誘発の結果、stx2fは誘発性ファージ上に位置し、ファージはレシピエント大腸菌にStx2f産生能を付与した。ドナー株のベロ毒素力価が増強された。

  • Piperine, an active ingredient of white pepper, suppresses the growth of multidrug‐resistant toxigenic Vibrio cholerae and other pathogenic bacteria Reviewed

    G.B. Manjunath, S.P. Awasthi, M.S.H. Zahid, N. Hatanaka, A. Hinenoya, E. Iwaoka, S. Aoki, T. Ramamurthy, S. Yamasaki

    Letters in Applied Microbiology   2022.02（ ISSN:0266-8254 ） （ eISSN:1472-765X ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/lam.13646

    Other URL： https://onlinelibrary.wiley.com/doi/full-xml/10.1111/lam.13646

  • Detection, isolation and molecular characterization of Escherichia albertii in wild birds in West Japan.

    Hinenoya A, Awasthi SP, Yasuda N, Nagano K, Hassan J, Takehira K, Hatanaka N, Saito S, Watabe T, Yoshizawa M, Inoue H, Yamasaki S

    Japanese Journal of Infectious Diseases   in press   2022

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    Kind of work：Joint Work  

  • Isolation and characterization of <i>Escherichia albertii</i> from wild and safeguarded animals in Okayama Prefecture and its prefectural borders, Japan Reviewed

    NAKA Atsushi, HINENOYA Atsushi, AWASTHI Sharda Prasad, YAMASAKI Shinji

    Journal of Veterinary Medical Science   84 ( 9 )   1299 - 1306   2022（ ISSN:09167250 ） （ eISSN:13477439 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：Domestic journal  

    <p><i>Escherichia albertii</i> has recently been recognized as a zoonotic enteropathogen associated with food poisoning. The reservoirs and transmission routes of this bacterium to humans are still unclear. In this study, we performed a survey of <i>E. albertii</i> in fecal specimens of wild and safeguarded animals in Okayama Prefecture and its prefectural borders, Japan to understand its reservoir in the environment. Forty-two <i>E. albertii</i> were isolated from 10 and 31 droppings of 59 crows and 125 starlings, respectively. Fifty-two <i>E. albertii</i> were isolated from 906 mammal droppings, and out of 52 isolates, origin of 33, 6 and 1 isolates were from martens, foxes, and rabbit, respectively, however, origin of 12 isolates remained unknown. Three <i>E. albertii</i> were isolated from two and one feces of 159 dogs and 76 cats, respectively. Pulsed-filed gel electrophoresis analysis grouped 97 <i>E. albertii</i> strains into 66 pulsotypes including 36 and 30 pulsotypes of isolates from mammals and birds, respectively. <i>E. albertii</i> strains isolated in this study were genetically diverse. Although clonal relationship was not observed between mammal and bird isolates, there were intra- and inter-species relationship in mammalian isolates. All <i>E. albertii</i> strains were positive for <i>eae</i> and <i>Eacdt</i> virulence genes. Furthermore, 20 and 7 strains also carried <i>Eccdt-I</i> and <i>stx2f</i> genes, respectively. Taken together, the results indicate that genetically diverse and potentially virulent <i>E. albertii</i> are distributed among various wild and safeguarded animals in Okayama Prefecture, and the animals could also be reservoirs of <i>E. albertii</i>.</p>

    DOI： 10.1292/jvms.22-0213

    PubMed

  • Identification of a recently dominant sublineage in Salmonella 4,[5],12:i:-sequence type 34 isolated from food animals in Japan.

    Arai N, Sekizuka T, Tamamura-Andoh Y, Barco L, Hinenoya A, Yamasaki S, Iwata T, Watanabe-Yanai A, Kuroda M, Akiba M, Kusumoto M

    Frontier in Microbiology   12   2021.07

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    Kind of work：Joint Work  

  • Prevalence, serovar and antimicrobial resistance of non-typhoidal Salmonella in vegetable, fruit and water samples in Ho Chi Minh City, Vietnam.

    Nguyen DTA, Awasthi SP, Hoang PH, Nguyen PD, Hassan J, Hatanaka N, Hinenoya A, Dang CV, Faruque SM, Yamasaki S

    Foodborne Pathog Dis   18 ( 5 )   354 - 363   2021.05

  • Isolation and characterization of Escherichia albertii in poultry at the pre-harvest level. Reviewed

    Atsushi Hinenoya, Xing-Ping Li, Ximin Zeng, Orhan Sahin, Rodney A Moxley, Catherine M Logue, Barbara Gillespie, Shinji Yamasaki, Jun Lin

    Zoonoses and public health   68 ( 3 )   213 - 225   2021.05（ ISSN:1863-1959 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Escherichia albertii, often misidentified as Escherichia coli, has become an emerging foodborne human enteric pathogen. However, the prevalence and major animal reservoirs of this significant pathogen are still not clear. Here, we performed comprehensive microbiological, molecular, comparative genomics and animal studies to understand the status and features of E. albertii in the US domestic and food animals. Although no E. albertii was identified in a total of 1,022 diverse E. coli strains isolated from pets and food animals in a retrospective screening, in a pilot study, E. albertii was successfully isolated from a broiler farm (6 out of 20 chickens). The chicken E. albertii isolates showed clonal relationship as indicated by both pulsed-field gel electrophoresis (PFGE) and whole-genome sequence analysis. The isolated chicken E. albertii displayed multidrug resistance; all the resistance determinants including the extended-spectrum beta-lactamase gene, carried by plasmids, could be conjugatively transferred to E. coli, which was further confirmed by S1-PFGE and Southern hybridization. Whole-genome sequence-based phylogenetic analysis showed the chicken E. albertii strains were phylogenetically close to those of human origins. Challenge experiment demonstrated that the E. albertii strains isolated from human and wild bird could successfully colonize in the chicken intestine. Together, this study, for the first time, reported the isolation of E. albertii in poultry at the pre-hrvest level. The findings from multi-tier characterization of the chicken E. albertii strains indicated the importance of chickens as a reservoir for E. albertii. A large scale of E. albertii survey in poultry production at the pre-harvest level is highly warranted in the future.

    DOI： 10.1111/zph.12812

    PubMed

  • Prevalence, O-genotype and Shiga toxin (Stx) 2 subtype of Stx-producing Escherichia coli strains isolated from Argentinean beef cattle. Reviewed

    Kentaro Okuno, Sharda Prasad Awasthi, Germán A Kopprio, Atsushi Iguchi, Noritoshi Hatanaka, Atsushi Hinenoya, Rubén José Lara, Shinji Yamasaki

    The Journal of veterinary medical science   83 ( 4 )   630 - 636   2021.04（ ISSN:0916-7250 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：Domestic journal  

    The aims of this study were to investigate prevalence, O-genotype, and virulence gene profile including Shiga toxin (Stx) 2 gene-subtype of Stx-producing Escherichia coli (STEC) in beef cattle from the Bahía Blanca in Argentina. Rectal swabs were collected from 283 beef cattle in 2012. stx genes were detected in 90 (32%) out of the 283 rectal swabs by stx gene-specific PCR assay. The positive cases were 13 with stx1, 58 with stx2, and 19 with both stx1 and stx2. Among 90 stx gene-positive samples, 45 STEC strains were isolated, which included 3 stx1, 34 stx2, and eight stx1 and stx2 genes positive isolates. O-genotyping grouped 45 STEC strains into 19 different O-genotypes such as Og8, Og145, Og171, Og185 (4 from each), Og22, Og153, Og157 (3 from each) and others. Various stx2 gene-subtypes were identified in 42 STEC strains: 13 positive cases for stx2a, 11 for stx2c, 3 for stx2g, 10 for stx2a and stx2d, 4 for stx2a and stx2c, and 1 for stx2b, stx2c and stx2g. efaI gene, generally prevalent in clinical strains, was detected in relatively high in the STEC strains. These data suggest that stx2a and stx2c were distributed not only in O145 and O157 but also in minor O-genotypes of STEC in Argentina.

    DOI： 10.1292/jvms.21-0002

    PubMed

  • Quantification of cholix exotoxin, an ADP-ribosylating factor in Vibrio cholerae strains by developed sandwich bead-ELISA. Reviewed

    Awasthi SP, Chowdhury N, Hatanaka N, Hinenoya A, Ramamurthy T, Asakura M, Yamasaki S

    J Med Microbiol   70 ( 4 )   2021.04

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    Kind of work：Joint Work  

  • アルゼンチンの肉用牛より単離された志賀毒素(Stx)産生大腸菌株の保菌率、O-遺伝子型及びStx2サブタイプ(Prevalence, O-genotype and Shiga toxin(Stx) 2 subtype of Stx-producing Escherichia coli strains isolated from Argentinean beef cattle)

    Okuno Kentaro, Awasthi Sharda Prasad, Kopprio German A., Iguchi Atsushi, Hatanaka Noritoshi, Hinenoya Atsushi, Lara Ruben Jose, Yamasaki Shinji

    The Journal of Veterinary Medical Science   83 ( 4 )   630 - 636   2021.04（ ISSN:0916-7250 ）

  • Critical Role of 3'-Downstream Region of pmrB in Polymyxin Resistance in Escherichia coli BL21(DE3). Reviewed

    Fuzhou Xu, Atsushi Hinenoya, Ximin Zeng, Xing-Ping Li, Ziqiang Guan, Jun Lin

    Microorganisms   9 ( 3 )   2021.03

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Polymyxins, such as colistin and polymyxin B, are the drugs used as a last resort to treat multidrug-resistant Gram-negative bacterial infections in humans. Increasing colistin resistance has posed a serious threat to human health, warranting in-depth mechanistic research. In this study, using a functional cloning approach, we examined the molecular basis of colistin resistance in Escherichia coli BL21(DE3). Five transformants with inserts ranging from 3.8 to 10.7 kb displayed significantly increased colistin resistance, three of which containing pmrB locus and two containing pmrD locus. Stepwise subcloning indicated that both the pmrB with a single G361A mutation and at least a 103 bp downstream region of pmrB are essential for conferring colistin resistance. Analysis of the mRNA level and stability showed that the length of the downstream region drastically affected the pmrB mRNA level but not its half-life. Lipid A analysis, by mass spectrometry, revealed that the constructs containing pmrB with a longer downstream region (103 or 126 bp) have charge-altering l-4-aminoarabinose (Ara4N) and phosphoethanolamine (pEtN) modifications in lipid A, which were not observed in both vector control and the construct containing pmrB with an 86 bp downstream region. Together, the findings from this study indicate that the 3'-downstream region of pmrB is critical for the PmrB-mediated lipid A modifications and colistin resistance in E. coli BL21(DE3), suggesting a novel regulatory mechanism of PmrB-mediated colistin resistance in E. coli.

    DOI： 10.3390/microorganisms9030655

    PubMed

  • Prevalence of mobile colistin resistance (mcr) genes in extended-spectrum β-lactamase-producing Escherichia coli isolated from retail raw foods in Nha Trang, Vietnam. Reviewed

    Phong Quoc Le, Sharda Prasad Awasthi, Noritoshi Hatanaka, Atsushi Hinenoya, Jayedul Hassan, Rabee Alhossiny Ombarak, Atsushi Iguchi, Nga Thuy Thi Tran, Khanh Van Thi Dao, Mai Quang Vien, Huy Xuan Le, Hung Thai Do, Yoshimasa Yamamoto, Shinji Yamasaki

    International journal of food microbiology   346   109164 - 109164   2021.03（ ISSN:0168-1605 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    The aim of the study was to assess the presence of genes in ESBL-producing E. coli (ESBL-Ec) isolated from retail raw food in Nha Trang, Vietnam. A total of 452 food samples comprising chicken (n = 116), pork (n = 112), fish (n = 112) and shrimp (n = 112) collected between 2015 and 2017 were examined for the prevalence of ESBL-Ec. ESBL-Ec were detected in 46.0% (208/452) of retail food samples, particularly in 66.4% (77/116), 55.4% (62/112), 42.0% (47/112) 19.6% (22/112) of chicken, pork, fish and shrimp, respectively. Sixty-five out of the 208 (31.3%) ESBL-Ec isolates were positive for mcr genes including mcr-1, mcr-3 and both mcr-1 and mcr-3 genes in 56/208 (26.9%), 1/208 (0.5%) and 8/208 (3.9%) isolates, respectively. Particularly, there was higher prevalence of mcr-1 in ESBL-Ec isolates from chicken (53.2%, 41/77) in comparison to shrimp (22.7%, 5/22), pork (11.3%, 7/62) and fish (6.4%, 3/47). mcr-3 gene was detected in co-existence with mcr-1 in ESBL-Ec isolates from shrimp (9.1%, 2/22), pork (8.1%, 5/62) and fish (2.1%, 1/47) but not chicken. The 65 mcr-positive ESBL-Ec (mcr-ESBL-Ec) were colistin-resistant with the MICs of 4-8 μg/mL. All mcr-3 gene-positive isolates belonged to group A, whereas phylogenetic group distribution of isolates harboring only mcr-1 was B1 (44.6%), A (28.6%) and D (26.8%). PFGE analysis showed diverse genotypes, although some isolates demonstrated nearly clonal relationships. S1-PFGE and Southern hybridization illustrated that the mcr-1 and mcr-3 genes were located either on chromosomes or on plasmids. However, the types of mcr genes were harbored on different plasmids with varied sizes of 30-390 kb. Besides, the ESBL genes of CTX-M-1 or CTX-M-9 were also detected to be located on plasmids. Noteworthy, co-location of CTX-M-1 with mcr-1 or mcr-3 genes on the same plasmid was identified. The conjugation experiment indicated that the mcr-1 or mcr-3 was horizontally transferable. All mcr-ESBL-Ec isolates were multidrug resistance (resistance to ≥3 antimicrobial classes). Moreover, β-Lactamase-encoding genes of the CTX-M-1 (78.5%), CTX-M-9 (21.5%), TEM (61.5%) groups were found in mcr-ESBL-Ec. The astA gene was detected in 27 (41.5%) mcr-ESBL-Ec isolates demonstrating their potential virulence. In conclusion, mcr-1 and mcr-3 genes existed individually or concurrently in ESBL-Ec isolates recovered from retail raw food in Nha Trang city, which might further complicate the antimicrobial-resistant situation in Vietnam, and is a possible health risk for human.

    DOI： 10.1016/j.ijfoodmicro.2021.109164

    PubMed

  • Critical role of 3'-downstream region of pmrB in polymyxin resistance in Escherichia coli BL21(DE3). Reviewed

    Fuzhou X, Hinenoya A, Zeng X, Li X-P, Guan Z, Lin J

    Microorganisms   9 ( 3 )   2021.03

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    Kind of work：Joint Work  

  • Prevalence of mobile colistin resistance (mcr) genes in extended-spectrum β-lactamase-producing Escherichia coli isolated from retail raw foods in Nha Trang, Vietnam.

    Le PQ, Awasthi SP, Hatanaka N, Hinenoya A, Hassan J, Ombarak RA, Iguchi A, Tran NTT, Dao KVT, Vien MQ, Le HX, Do HT, Yamamoto Y, Yamasaki S

    Int J Food Microbiol   346   2021.03

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    Kind of work：Joint Work  

  • Isolation and characterization of Escherichia albertii in poultry at the pre-harvest level. Reviewed

    Hinenoya A, Li X-P, Zeng X, Sahin O, Moxley RA, Logue CM, Gillespie B, Yamasaki S, Lin J

    Zoonoses and Public Health   2021.02

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    Kind of work：Joint Work  

  • Accurate identification of Escherichia albertii by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Reviewed

    Hatanaka N, Awasthi SP, Hinenoya A, Ueda O, Yamasaki S

    Journal of Microbiological Methods   173   2020.06

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    Kind of work：Joint Work  

  • Prevalence of Escherichia albertii in raccoons (Procyon lotor) in Japan Reviewed

    Hinenoya A, Nagano K, Awasthi SP, Hatanaka N, Yamasaki S

    Emerging and Infectious Diseases   26 ( 6 )   1304 - 1307   2020.06

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    Kind of work：Joint Work  

  • Prevalence of Escherichia albertii in Raccoons (Procyon lotor), Japan. Reviewed

    Atsushi Hinenoya, Keigo Nagano, Sharda P Awasthi, Noritoshi Hatanaka, Shinji Yamasaki

    Emerging infectious diseases   26 ( 6 )   1304 - 1307   2020.06（ ISSN:1080-6040 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Natural reservoirs of Escherichia albertii remain unclear. In this study, we detected E. albertii by PCR in 248 (57.7%) of 430 raccoons from Osaka, Japan, and isolated 143 E. albertii strains from the 62 PCR-positive samples. These data indicate that raccoons could be a natural reservoir of E. albertii in Japan.

    DOI： 10.3201/eid2606.191436

    PubMed

  • Chlorous acid is a more potent antibacterial agent than sodium hypochlorite against Campylobacter. Reviewed

    Hatanaka N, Awasthi SP, Goda H, Kawata H, Uchino Y, Kubo T, Aoki S, Hinenoya A, Yamasaki S

    Food Control   111   2020.05

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    Kind of work：Joint Work  

  • Development of XRM-MacConkey agar selective medium for the isolation of Escherichia albertii. Reviewed

    Hinenoya A, Nagano K, Okuno K, Nagita A, Hatanaka N, Awasthi SP, Yamasaki S

    Diagnostic Microbiology and Infectious Disease   97 ( 1 )   2020.05

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    Kind of work：Joint Work  

  • Development of XRM-MacConkey agar selective medium for the isolation of Escherichia albertii. Reviewed

    Atsushi Hinenoya, Keigo Nagano, Kentaro Okuno, Akira Nagita, Noritoshi Hatanaka, Sharda Prasad Awasthi, Shinji Yamasaki

    Diagnostic microbiology and infectious disease   97 ( 1 )   115006 - 115006   2020.05（ ISSN:0732-8893 ）

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    International / domestic magazine：International journal  

    Escherichia albertii has increasingly been recognized as an emerging pathogen. However, lack of selective medium for E. albertii is the bottleneck for clinical and epidemiological investigations. In this study, a selective medium for E. albertii named XRM-MacConkey agar, which is modified MacConkey agar supplemented with xylose (X), rhamnose (R), and melibiose (M) instead of lactose, was developed and evaluated. All 49 E. albertii and 6 different species out of 23 grew as colorless colonies, whereas 17 remaining species grew as red colonies. Detection limit of E. albertii by this medium was 105 CFU/g stool when examined with spiked healthy human stool. Furthermore, colorless colonies on XRM-MacConkey agar obtained from 7 E. albertii-positive diarrheal stools were consistently E. albertii. In contrast, 57%, 18%, and 36% colorless colonies on MacConkey, DHL, and mEA agars, respectively, were non-E. albertii. We concluded that XRM-MacConkey agar could specifically be used for isolation of E. albertii.

    DOI： 10.1016/j.diagmicrobio.2020.115006

    PubMed

  • Chlorous acid is a more potent antibacterial agent than sodium hypochlorite against Campylobacter Reviewed

    Noritoshi Hatanaka, Sharda Prasad Awasthi, Hisataka Goda, Hiroyuki Kawata, Yuzuru Uchino, Takahiro Kubo, Shigeru Aoki, Atsushi Hinenoya, Shinji Yamasaki

    Food Control   111   2020.05（ ISSN:0956-7135 ）

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    Publishing type：Research paper (scientific journal)  

    © 2019 Elsevier Ltd Foodborne disease caused by campylobacters is one of the major global problems for food safety. Infection source of Campylobacter to human is mainly through contaminated meat particularly chicken. Contamination of meat with Campylobacter usually occurs during processing at slaughterhouse and to prevent such contaminations, sodium hypochlorite is commonly used. However, it is well known that bactericidal activity of sodium hypochlorite becomes weak under organic matter rich conditions. In this study, we compared the strength of bactericidal activity of chlorous acid and sodium hypochlorite against Campylobacter jejuni and Campylobacter coli strains under organic matter rich conditions. Bactericidal activity against 5 representative C. jejuni and C. coli strains in chicken juice (an organic matter rich condition) showed that minimum concentration of chlorous acid required for complete killing of C. jejuni and C. coli cells is 200–400 ppm while that of sodium hypochlorite is 2,000 to 4,000 ppm. Similar results were obtained by using Bolton broth. Furthermore, it was observed that 400 ppm of chlorous acid but not 400 ppm of sodium hypochlorite is highly effective in killing of 25 different Campylobacter strains (12 C. jejuni and 13 C. coli strains) under the same conditions. To determine whether 400 ppm of chlorous acid treatment had killed bacterial cells or induced them into viable but non-culturable (VBNC) state, live and dead cell assay using DAPI and propidium iodide fluorescent dyes was done. Such assay clearly indicated that Campylobacter cells were indeed killed and not induced to VBNC state. Moreover, SDS-PAGE analysis of whole-cell lysates of campylobacters indicated distinct effects in protein profiles of chlorous acid but not sodium hypochlorite treated cells. The results strongly suggest that chlorous acid could efficiently kill C. jejuni and C. coli cells with much lower concentration than sodium hypochlorite and the bactericidal mechanisms of chlorous acid may be due to damages of bacterial proteins. Thus, chlorous acid could be a better disinfectant in chicken slaughtering and processing to kill campylobacters and prevent contamination.

    DOI： 10.1016/j.foodcont.2019.107046

  • Accurate identification of Escherichia albertii by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Reviewed

    Noritoshi Hatanaka, Sharda Prasad Awasthi, Atsushi Hinenoya, Osamu Ueda, Shinji Yamasaki

    Journal of microbiological methods   173   105916 - 105916   2020.04（ ISSN:0167-7012 ）

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    International / domestic magazine：International journal  

    A specific identification protocol for Escherichia albertii by using a MALDI-TOF/MS method was developed. For this purpose, a novel database was established which can differentiate E. albertii from E. coli by combining the mass spectra obtained from 58 E. albertii and 36 E. coli strains.

    DOI： 10.1016/j.mimet.2020.105916

    PubMed

  • Development of a specific cytolethal distending toxin (cdt) gene (Eacdt)-based PCR assay for the detection of Escherichia albertii. Reviewed

    Hinenoya A, Ichimura H, Yasuda N, Harada S, Yamada K, Suzuki M, Iijima Y, Nagita A, Albert MJ, Hatanaka N, Awasthi SP, Yamasaki S

    Diagn Microbiol Infect Dis 雑誌 ELSEVIER   95 ( 2 )   119 - 124   2019.10

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    Kind of work：Single Work  

  • Serotypes, pathogenic potential and antimicrobial resistance of Escherichia coli isolated from subclinical bovine mastitis milk samples in Egypt. Reviewed

    Ombarak RA, Zayda MG, Awasthi SP, Hinenoya A, Yamasaki S

    Jpn J Infect Dis 雑誌 国立感染症研究所   72 ( 5 )   337 - 339   2019.09

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    Kind of work：Joint Work  

  • Extended-spectrum beta-lactamase-producing Escherichia coli harboring sul and mcr-1 genes isolates from fish gut contents in the Mekong delta, Vietnam. Reviewed

    Tran HTT, Nakayama T*, Huyen HM, Harada K, Hinenoya A, Phuong NT, Yamamoto Y.

    Letters in Applied Microbiology   71   78 - 85   2019.09

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    Kind of work：Joint Work  

  • Serotypes, Pathogenic Potential, and Antimicrobial Resistance of Escherichia coli Isolated from Subclinical Bovine Mastitis Milk Samples in Egypt. Reviewed

    Ombarak RA, Zayda MG, Awasthi SP, Hinenoya A, Yamasaki S

    Japanese journal of infectious diseases   72 ( 5 )   337 - 339   2019.09（ ISSN:1344-6304 ）

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  • Salmonella genomic island 3 is an integrative and conjugative element and contributes to copper and arsenic tolerance of Salmonella enterica. Reviewed

    Arai N, Sekizuka T, Tamamura Y, Kusumoto M, Hinenoya A, Yamasaki S, Iwata T, Watanabe-Yanai A, Kuroda M, Akiba M

    Appl. Environ Microbiol 雑誌 American Society for Microbiology   63 ( 9 )   pii: e00429 - 19   2019.08

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    Kind of work：Joint Work  

  • Evaluation of the GeneFields® EHEC/SS PCR dipstick DNA chromatography kit for the detection of enteric bacterial pathogens in stool specimens of healthy humans. Reviewed

    Maruoka H, Hinenoya A, Yasuda N, Takeda A, Inoue S, Sumi T, Koitabashi K, Yasue H, Kogou K, Yamasaki S

    J Microbiol Methods 雑誌 ELSEVIER   161   111 - 117   2019.06

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    Kind of work：Joint Work  

  • Comparison of Established PCR Assays for Accurate Identification of Campylobacter jejuni and Campylobacter coli. Reviewed

    S M Lutful Kabir, Nityananda Chowdhury, Masahiro Asakura, Sachi Shiramaru, Ken Kikuchi, Atsushi Hinenoya, Sucharit Basu Neogi, Shinji Yamasaki

    Japanese journal of infectious diseases   72 ( 2 )   81 - 87   2019.03（ ISSN:1344-6304 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：Domestic journal  

    Proper surveillance of Campylobacter jejuni and Campylobacter coli, major pathogens associated with human gastroenteritis, is necessary to tackle the increasing disease burden. To detect these pathogenic species, a variety of PCR assays have been developed. This study examined the sensitivity and specificity of 12 PCR assays targeting 23S rRNA, ceuE, lpxA, hipO, mapA, ask, and cdt genes of C. jejuni and C. coli. The sensitivities of PCR assays were 85.2-100%, and 97-100%, and the specificities were 90.5-100%, and 94.3-100% for the tested C. jejuni (n = 61) and C. coli (n = 33) strains, respectively. Two PCR assays, targeting cdtC and hipO genes, were found to be 100% sensitive and/or specific for all C. jejuni strains, while 3 assays, targeting cdtB, cdtA, and ask genes, were 100% sensitive and/or specific for C. coli strains. However, PCR assays for hipO and ask genes are problematic to conduct simultaneously due to the differences in PCR conditions. Overall, multiplex PCR assays targeting cdtC and cdtB genes, encoding 2 subunits of the same toxin, were concluded to be the most reliable. The results of this study would aid in proper surveillance of C. jejuni and C. coli and adopting intervention strategies in the near future.

    DOI： 10.7883/yoken.JJID.2018.340

    PubMed

  • Phenotypic and molecular characterization of Escherichia albertii: Further surrogates to avoid potential laboratory misidentification. Reviewed

    Hinenoya A, Ichimura H, Awasthi SP, Yasuda N, Yatsuyanagi J, Yamasaki S

    Int J Med Microbiol 雑誌 ELSEVIER   309 ( 2 )   108 - 115   2019.03

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    Kind of work：Single Work  

  • Comparison of established PCR assays for accurate identification of Campylobacter jejuni and Campylobacter coli. Reviewed

    Kabir SML, Chowdhury N, Asakura M, Shiramaru S, Kikuchi K, Hinenoya A, Neogi SB, Yamasaki S

    Jpn J Infect Dis 雑誌 国立感染症研究所   72 ( 2 )   81 - 87   2019.03

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    Kind of work：Joint Work  

  • Phenotypic and molecular characterization of Escherichia albertii: Further surrogates to avoid potential laboratory misidentification. Reviewed

    Hinenoya A, Ichimura H, Awasthi SP, Yasuda N, Yatsuyanagi J, Yamasaki S

    International journal of medical microbiology : IJMM   309 ( 2 )   108 - 115   2019.03（ ISSN:1438-4221 ）

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  • Development of a bivalent food poisoning vaccine: augmented antigenicity of the C-terminus of Clostridium perfringens enterotoxin by fusion with the B subunit of Escherichia coli Shiga toxin 2. Reviewed

    Hosomi K, Hinenoya A, Suzuki H, Nagatake T, Nishino T, Tojima Y, Hirata SI, Matsunaga A, Kondoh M, Yamasaki S, Kunisawa J

    International immunology   31 ( 2 )   91 - 100   2019.03（ ISSN:0953-8178 ） （ eISSN:1460-2377 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/intimm/dxy071

    PubMed

  • Campylobacter jejuniとCampylobacter coliの正確な同定に用いられるPCRアッセイの比較(Comparison of Established PCR Assays for Accurate Identification of Campylobacter jejuni and Campylobacter coli)

    Kabir S.M. Lutful, Chowdhury Nityananda, Asakura Masahiro, Shiramaru Sachi, Kikuchi Ken, Hinenoya Atsushi, Neogi Sucharit Basu, Yamasaki Shinji

    Japanese Journal of Infectious Diseases   72 ( 2 )   81 - 87   2019.03（ ISSN:1344-6304 ）

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    Campylobacter jejuniとCampylobacter coliの同定に用いられる12種類のPCRアッセイ法の感度と特異度を比較検討した。日本国内で分離されたカンピロバクター菌のうち、C.jejuni 58株、C.coli 31株を分析対象とした。検討の結果、PCRアッセイによって菌検出の有用性に関して大きな差が認められた。C.jejuniの検出に際して、ceuE遺伝子ベースのアッセイでは感度は85%にとどまり、cdtC、hipO、IpxAの各遺伝子をベースとしたアッセイでは感度100%、cdtA、cdtB、mapA、23S rRNA遺伝子ベース解析では感度は92〜97%を示していた。一方、C.coliに対しては各PCRアッセイの検出率は良好であり、ceuEとcdtA遺伝子ベース解析で感度が97%を示したほか、各アッセイの感度は100%であった。cdtA、cdtBおよびcdtCをベースとする多重PCRアッセイでは、C.jejuniとC.coliの同時検出における感度は100%を示し、hipO、ask、ceuEを用いた多重アッセイでも同様の成績が得られたが、IpxAと23S rRNAを用いたアッセイでは偽陽性が認められ感度は低くなっていた。今回検討したPCRアッセイのうち、cdtCとcdtBの両遺伝子を標的とする多重PCRアッセイが最も信頼性が高い検出法であると考えられた。

  • Development of a bivalent food poisoning vaccine: augmented antigenicity of the C-terminus of Clostridium perfringens enterotoxin by fusion with the B subunit of Escherichia coli Shiga toxin 2. Reviewed

    Hosomi K, Hinenoya A, Suzuki H, Nagatake T, Nishino T, Tojima Y, Hirata SI, Matsunaga A, Kondoh M, Yamasaki S, Kunisawa J

    Int Immunol 雑誌 Oxford Academic   31 ( 2 )   91 - 100   2019.02

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    Kind of work：Joint Work  

  • Development of a multiplex PCR assay for the detection of major virulence genes in Vibrio cholerae including non-O1 and non-O139 serogroups. Reviewed

    Awasthi SP, Chowdhury N, Neogi SB, Hinenoya A, Hatanaka N, Chowdhury G, Ramamurthy T, Yamasaki S

    J Microbiol Methods 雑誌 ELSEVIER   157   54 - 58   2019.02

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    Kind of work：Joint Work  

  • Development of a multiplex PCR assay for the detection of major virulence genes in Vibrio cholerae including non-O1 and non-O139 serogroups.

    Awasthi SP, Chowdhury N, Neogi SB, Hinenoya A, Hatanaka N, Chowdhury G, Ramamurthy T, Yamasaki S

    Journal of microbiological methods   157   54 - 58   2019.02（ ISSN:0167-7012 ）

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  • Characterization of Vibrio cholerae isolates from two distinct Kenyan cholera outbreaks in 2007 and 2009 Reviewed

    S. P. Awasthi, S. M. Saidi, N. Chowdhury, M. Asakura, P. H. Hoang, A. Hinenoya, D. Motooka, S. Nakamura, Y. Iijima, S. Yamasaki

    2019.02

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    Publishing type：Research paper (international conference proceedings)  

  • 2価食中毒ワクチンの開発　志賀毒素2型産生大腸菌Bサブユニットとの融合によりウエルシュ菌エンテロトキシンC末端の抗原性が増強される(Development of a bivalent food poisoning vaccine: augmented antigenicity of the C-terminus of Clostridium perfringens enterotoxin by fusion with the B subunit of Escherichia coli Shiga toxin 2)

    Hosomi Koji, Hinenoya Atsushi, Suzuki Hidehiko, Nagatake Takahiro, Nishino Tomomi, Tojima Yoko, Hirata So-ichiro, Matsunaga Ayu, Kondoh Masuo, Yamasaki Shinji, Kunisawa Jun

    International Immunology   31 ( 2 )   91 - 100   2019.02（ ISSN:0953-8178 ）

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    今回、ウエルシュ菌や志賀毒素(Stx)産生大腸菌(STEC)感染により誘発される重篤な全身症状や、致死性ウエルシュ菌エンテロトキシン(CPE)およびStxに対し感染防御効果を示す2価ワクチンを開発した。なお、開発したワクチンは、志賀毒素2型のBサブユニット(Stx2B)とCPEのC末端(C-CPE)を融合(Stx2B-C-CPE)させて作製し、C-CPEの抗原性を増強させたことを特徴とした。本ワクチンをStx2B-C-CPEで免疫したマウスに接種させ、有効性を評価した結果、中和試験により、高レベルのC-CPE特異的IgGおよび相当量のStx2B特異的IgGが誘導され、これらの抗体応答が最低48週間継続したことから、本ワクチンが長期間の防御免疫を誘導する可能性が示唆された。さらに、Stx2B-C-CPEを用いた免疫応答では、Stx2B刺激によりStx2B特異的T細胞が誘導され、IgMからIgGへ、Stx2B-およびC-CPE-特異的B細胞において、免疫グロブリン分子のクラススイッチが促進されるメカニズムも確認された。以上の所見から、ウエルシュ菌およびSTEC感染に対する本ワクチンの有効性が立証された。

  • Novel cholera toxin variant and ToxT regulon in environmental Vibrio mimicus strains: potential resources for the evolution of Vibrio cholerae hybrid strains. Reviewed

    Neogi SB, Chowdhury N, Awasthi SP, Asakura M, Okuno K, Mahmud ZH, Islam MS, Hinenoya A, Nair GB, Yamasaki S

    Appl Environ Microbiol 雑誌 American Society for Microbiology   85 ( 3 )   pii: e01977 - 18   2019.01

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    Kind of work：Joint Work  

  • Extended-spectrum beta-lactamase-producing Escherichia coli harbouring sul and mcr-1 genes isolates from fish gut contents in the Mekong Delta, Vietnam Reviewed

    T. T.T. Hoa, T. Nakayama, H. M. Huyen, K. Harada, A. Hinenoya, N. T. Phuong, Y. Yamamoto

    Letters in Applied Microbiology   2019（ ISSN:0266-8254 ） （ eISSN:1472-765X ）

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    Publishing type：Research paper (scientific journal)  

    © 2019 The Society for Applied Microbiology This study investigated the existence of sulfonamides and colistin resistance genes among extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli recovered from fish gut in Vietnam and evaluated the susceptibility patterns of the ESBL-producing E. coli to relevant antimicrobials. A total of 88 ESBL-producing E. coli isolates were analysed for the presence of the ESBLs, sul (1, 2, 3) and mcr (1–3) genes by PCR. Antimicrobial resistance phenotypes of isolates were determined by disc diffusion. Results showed that: (i) A high prevalence of 94·3% of sulfonamide resistance was observed in 88 isolates. Moreover, the existence of 2·3% of ESBL-producing E. coli harbouring mcr-1 gene were detected; (ii) The phylogenetic types A and B1 were most frequent, and the blaCTX-M group1 and blaTEM genes encoding ESBL were detected in 47·7% of the isolates; (iii) ESBL-producing E. coli harbouring mcr-1 gene exhibited resistance to 11 antibiotics. The existence of mcr-1 and sul1,2,3 genes and the extremely high level of multiple drug resistance in all ESBL-producing E. coli isolates obtained from sampled fish in Vietnam is a major concern. Therefore, it is imperative to monitor ESBL-producing E. coli in the river waters of Vietnam.

    DOI： 10.1111/lam.13222

    PubMed

  • Development of a multiplex PCR targeting eae, stx and cdt genes in genus Escherichia and detection of a novel cdtB gene in Providencia rustigianii. Reviewed

    Hassan J, Awasthi SP, Hatanaka N, Okuno K, Hoang PH, Nagita A, Hinenoya A, Yamasaki S

    Pathogens and Disease 雑誌 Oxford Academic   76 ( 9 )   2018.12

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  • MCR-1 confers cross-resistance to Bacitracin, a widely used in-feed antibiotic. Reviewed

    Xu F, Zeng X, Hinenoya A, Lin J

    mSphere 雑誌 American Society for Microbiology   3 ( 5 )   pii: e00411 - 18   2018.09

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    Kind of work：Joint Work  

  • Phylogenetic characterization of Salmonella enterica serovar Typhimurium and its monophasic variant isolated from food animals in Japan revealed replacement of major epidemic clones in the last four decades. Reviewed

    Arai N, Sekizuka T, Tamamura Y, Tanaka K, Barco L, Izumiya H, Kusumoto M, Hinenoya A, Yamasaki S, Iwata T, Watanabe A, Kuroda M, Uchida I, Akiba M

    J Clin Microbiol 雑誌 American Society for Microbiology   56 ( 5 )   pii: e01758 - 17   2018.04

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  • Prevalence and characterization of extended-spectrum β-lactamases-producing Escherichia coli in domestic and imported chicken meats in Japan. Reviewed

    Nahar A, Awasthi SP, Hatanaka N, Okuno K, Hoang PH, Hassan J, Hinenoya A, Yamasaki S

    J Vet Med Sci 雑誌 Japanese Society of Veterinary Science   80 ( 3 )   510 - 517   2018.03

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    Kind of work：Joint Work  

  • Isolation and molecular characterization of extended-spectrum β-lactamase (ESBL) producing Escherichia coli from industrial food animals in Mekong Delta, Vietnam. Reviewed

    Hinenoya A, Tran Thi Thu S, Nguyen NT, Nguyen HC, Le Nguyen DD, Hoang PH, Awasthi SP, Hassan J, Sumimura Y, Yamamoto Y, Yamasaki S

    Jpn J Vet Res 雑誌 Faculty of Veterinary Medicine, Hokkaido University   66 ( 1 )   1 - 12   2018.02

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    Kind of work：Single Work  

  • Prevalence and molecular characterization of antimicrobial resistance in Escherichia coli isolated from raw milk and raw milk cheese in Egypt. Reviewed

    Ombarak RA, Hinenoya A, Elbagory AM, Yamasaki S

    J Food Prot 雑誌 International Association for Food Protection   81 ( 2 )   226 - 232   2018.02

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    Kind of work：Joint Work  

  • Association of cytolethal distending toxin-II gene-positive Escherichia coli with Escherichia albertii, an emerging zoonotic pathogen. Reviewed

    Hinenoya A, Yasuda N, Mukaizawa N, Sheikh S, Niwa Y, Awasthi SP, Asakura M, Tsukamoto T, Nagita A, Albert MJ, Yamasaki S

    Int J Med Microbiol 雑誌 ELSEVIER   307 ( 8 )   564 - 571   2017.12

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    Kind of work：Single Work  

  • High prevalence of Campylobacter ureolyticus in stool specimens of children with diarrhea in Japan. Reviewed

    Hatanaka N, Shimizu A, Somroop S, Li Y, Asakura M, Nagita A, Awasthi SP, Hinenoya A, Yamasaki S

    Jpn J Infect Dis 雑誌 国立感染症研究所   70 ( 4 )   455 - 457   2017.07

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  • Isolation and characterization of an Escherichia albertii producing three different toxins from a child with diarrhea. Reviewed

    Hinenoya A, Yasuda N, Hibino T, Shima A, Nagita A, Tsukamoto T, Yamasaki S

    Jpn J Infect Dis 雑誌 国立感染症研究所   70 ( 3 )   252 - 257   2017.05

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  • Campylobacter upsaliensis isolated from dog produces high titer of cytolethal distending toxin. Reviewed

    Somroop S, Hatanaka N, Awasthi SP, Okuno K, Asakura M, Hinenoya A, Yamasaki S

    J Vet Med Sci 雑誌 Japanese Society of Veterinary Science   79 ( 3 )   683 - 691   2017.03

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    Kind of work：Joint Work  

  • Water metagenomic analysis reveals low bacterial diversity and the presence of antimicrobial residues and resistance genes in a river containing wastewater from backyard aquacultures in the Mekong Delta, Vietnam. Reviewed

    Nakayama T, Tuyet Hoa TT, Harada K, Warisaya M, Asayama M, Hinenoya A, Lee JW, Phu TM, Ueda S, Sumimura Y, Hirata K, Phuong NT, Yamamoto Y.

    Environ Pollut 雑誌 ELSEVIER   222   294 - 306   2017.03

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  • Antimicrobial resistance profiles and molecular characterization of Escherichia coli strains isolated from healthy adults in Ho Chi Minh City, Vietnam. Reviewed

    Hoang PH, Awasthi SP, DO Nguyen P, Nguyen NL, Nguyen DT, LE NH, VAN Dang C, Hinenoya A, Yamasaki S

    J Vet Med Sci 雑誌 Japanese Society of Veterinary Science   79 ( 3 )   479 - 485   2017.03

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  • A PCR-RFLP assay to detect and type cytolethal distending toxin (cdt) genes in Campylobacter hyointestinalis. Reviewed

    Hatanaka N, Kamei K, Somroop S, Awasthi SP, Asakura M, Misawa N, Hinenoya A, Yamasaki S

    J Vet Med Sci 雑誌 Japanese Society of Veterinary Science   79 ( 2 )   336 - 342   2017.02

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  • Anethole inhibits growth of recently emerged multidrug resistant toxigenic Vibrio cholerae O1 El Tor variant strains in vitro Reviewed

    M.S. Zahid, S.P. Awasthi, A. Hinenoya, S. Yamasaki

    J Vet Med Sci 雑誌 Japanese Society of Veterinary Science   77 ( 5 )   535 - 540   2015.06

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  • Prevalence of Providencia strains among patients and retail meats in Thailand Reviewed

    A. Shima, A. Hinenoya, W. Samosornsuk, S. Samosornsuk, S. Yamasaki

    Jpn J Infect Dis 雑誌 国立感染症研究所   2015

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  • Chlorine dioxide is a superior disinfectant against multi-drug resistant Staphylococcus aureus, Pseudomonas aeruginosa and Acinetobacter baumannii Reviewed

    A. Hinenoya, S.P. Awasthi, N. Yasuda, A. Shima, H. Morino, T. Koizumi, T. Fukuda, T. Miura, T. Shibata, S. Yamasaki

    Jpn J Infect Dis 雑誌 国立感染症研究所   2015

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  • Cytolethal distending toxin gene-based multiplex PCR assay for Campylobacter jejuni, C. coli, C. fetus, C. upsaliensis, C. hyointestinalis and C. lari Reviewed

    K. Kamei, H. Kawabata, M. Asakura, W. Samosornsuk, A. Hinenoya, S. Nakagawa, S. Yamasaki

    Jpn J Infect Dis 雑誌 国立感染症研究所   2015

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  • Development of a PCR-restriction fragment length polymorphism assay for detection and subtyping of cholix toxin variant genes of Vibiro cholerae Reviewed

    S.P. Awasthi, M. Asakura, S.B. Neogi, A. Hinenoya, T. Ramamurthy, S. Yamasaki

    J. Med. Microgiol. Society for General Microbiology   63 ( 5 )   667 - 673   2014.05

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  • A PCR-RFLP assay for the detection and differentiation of Campylobacter jejuni, C. coli, C. fetus, C. hyointestinalis, C. lari, C. helveticus and C. upsaliensis Reviewed

    K. Kamei, M. Asakura, S. Somroop, N. Hatanaka, A. Hinenoya, A. Nagita, N. Misawa, M. Matsuda, S. Nakagawa, S. Yamasaki

    J. Med. Microgiol. Society for General Microbiology   63 ( 5 )   659 - 666   2014.05

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  • Development of a PCR-restriction fragment length polymorphism assay for detection and subtyping of cholix toxin variant genes of Vibrio cholerae Reviewed

    S.P. Awasthi, M. Asakura, S.B. Neogi, A. Hinenoya, T. Ramamurthy, S. Yamasaki

    J Med Microbiol 雑誌 Society for General Microbiology   63 ( 5 )   667 - 673   2014.05

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    Kind of work：Joint Work  

  • Molecular characterization of cytolethal distending toxin gene-positive Escherichia coli from healthy cattle and swine in Nara, Japan Reviewed

    A. Hinenoya, K. Shima, M. Asakura, K. Nishimura, T. Tsukamoto, T. Ooka, T. Hayashi, T. Ramamurthy, S.M. Faruque, S. Yamasaki

    BMC Microbiol 雑誌 BioMed Central Ltd.   14   2014.04

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  • Prevalence of Vibrio cholerae O1 El Tor variant in a cholera-epidemic zone of Kenya Reviewed

    S.M. Saidi, N. Chowdhury, S.P. Awasthi, M. Asakura, A. Hinenoya, Y. Iijima, S. Yamasaki

    J. Med. Microgiol. Society for General Microbiology   63 ( 3 )   415 - 420   2014.03

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    Kind of work：Joint Work  

  • Novel cholix toxin variants, ADP-ribosylating in Vibrio cholerae non-O1/non-O139 strains, and their pathogenicity Reviewed

    S.P. Awasthi, M. Asakura, N. Chowdhury, S.B. Neogi, A. Hinenoya, H.M. Golbar, J. Yamate, E. Arakawa, T. Tada, T. Ramamurthy, S. Yamasaki

    Infect. Immun. American Society for Microbiology   81 ( 2 )   531 - 541   2013.02

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  • Molecular characterizations of cytolethal distending toxin produced by Providencia alcalifaciens strains isolated from patients with diarrhea Reviewed

    A. Shima, A. Hinenoya, M. Asakura, N. Sugimoto, T. Tsukamoto, H. Ito, A. Nagita, S.M. Faruque and S. Yamasaki

    Infect. Immun. American Society for Microbiology   80 ( 4 )   1323 - 1332   2012.04

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    Kind of work：Joint Work  

  • Prevalence of Providencia strains among children with diarrhea in Japan Reviewed

    A. Shima, A. Hinenoya, M. Asakura, A. Nagita, S. Yamasaki

    Jpn. J. Infect. Dis. National Institute of Infectious Diseases   65 ( 6 )   545 - 547   2012

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    Kind of work：Joint Work  

  • Evaluation of a PCR-restriction fragment length polymorphism (PCR-RFLP) assay for molecular epidemiological study of Shiga toxin-producing Escherichia coli Reviewed

    N. Sugimoto, K. Shima, A. Hinenoya, M. Asakura, A. Matsuhisa, H. Watanabe and S. Yamasaki

    J. Vet. Med. Sci. Japanese Society of Veterinary Science   73 ( 7 )   859 - 867   2011.07

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    Kind of work：Joint Work  

  • Inhibition of virulence potential of Vibrio cholerae by natural compounds

    S. Yamasaki, M. Asakura, S. B. Neogi, A. Hinenoya, E. Iwaoka and S. Aoki

    Indian J. Med. Res. Indian Journal of Medical Research   133   232 - 239   2011.02

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    Kind of work：Joint Work  

  • Evaluation of a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the identification of Campylobacter strains isolated from diarrheal patients in Japan Reviewed

    S. M. Kabir, K. Kikuchi, M. Asakura, S. Shiramaru, N. Tsuruoka, A. Goto, A. Hinenoya and S. Yamasaki

    Jpn. J. Infect. Dis. National Institute of Infectious Diseases   64 ( 1 )   19 - 27   2011.01

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    Kind of work：Joint Work  

  • Identification of Vibrio campbellii isolated from diseased farm-shrimps from south India and establishment of its pathogenic potential in an Artemia model. Reviewed

    S. Haldar, S. Chatterjee, N. Sugimoto, S. Das, N. Chowdhury, A. Hinenoya, M. Asakura and S. Yamasaki

    Microbiology Society for General Microbiology   157 ( 1 )   179 - 188   2011.01

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    Kind of work：Joint Work  

  • Candida albicans, Cryptococcus neoformans or Aspergillus fumigatus induces an antifungal activity in mouse serum, which is different from transferrin Reviewed

    K. Okazaki, M. Asakura, N. Sugimoto, A. Hinenoya and S. Yamasaki

    J. Vet. Med. Sci. Japanese Society of Veterinary Science   71 ( 11 )   1459 - 1464   2010.11

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    Kind of work：Joint Work  

  • Molecular epidemiology of Vibrio cholerae and Campylobacter isolated in Asian countries.

    S. Yamasaki , M.Asakura , S. Shiramaru, S.B. Neogi, A. Hinenoya, W. Samosornsuk, L. Shi and T. Ramamurthy

    Current Topics of Infectious Diseases in Japan and Asia Springer   1   25 - 43   2010.10

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    Kind of work：Joint Work  

  • Identification of Vibrio harveyi as a causative bacterium for tail rot disease of sea bream Sparus aurata from research hatchery in Malta. Reviewed

    S. Haldar, A. Maharajan, S. Chatterjee, S.A. Hunter, N. Chowdhury, A. Hinenoya, M. Asakura and S. Yamasaki

    Microbiol. Res. ELSEVIER   165 ( 8 )   639 - 648   2010.10

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    Kind of work：Joint Work  

  • A highly sensitive and specific multiplex PCR assay for simultaneous detection of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus. Reviewed

    S.B. Neogi, N. Chowdhury, M. Asakura, A. Hinenoya, S. Haldar, S.M. Saidi, K. Kogure, R.J. Lara and S. Yamasaki

    Lett. Appl. Microbiol. Wiley-Blackwell   51 ( 3 )   293 - 300   2010.09

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    Kind of work：Joint Work  

  • Development of simple and rapid PCR-fingerprinting methods for Vibrio cholerae on the basis of genetic diversity of the superintegron. Reviewed

    N. Chowdhury, M. Asakura, S.B. Neogi, A. Hinenoya, S. Haldar, T. Ramamurthy, B.L. Sarkar, S.M. Faruque and S. Yamasaki

    J. Appl. Microbiol. Wiley-Blackwell   109 ( 1 )   304 - 312   2010.07

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    Kind of work：Joint Work  

  • Distribution of virulence genes related to adhesions and toxins in Shiga toxin-producing Escherichia coli strains isolated from healthy cattle and diarrheal patients in Japan Reviewed

    Y. Wu, A. Hinenoya, T. Taguchi, A. Nagita, K. Shima, T. Tsukamoto, N. Sugimoto, M. Asakura and S. Yamasaki

    J. Vet. Med. Sci. Japanese Society of Veterinary Science   72 ( 5 )   589 - 597   2010.05

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    Kind of work：Joint Work  

  • Capsaicin, a potential inhibitor of cholera toxin production in Vibrio cholerae. Reviewed

    S. Chatterjee, M. Asakura, N. Chowdhury, S.B. Neogi, N. Sugimoto, S. Haldar, S.P. Awasthi, A. Hinenoya, S. Aoki and S. Yamasaki

    FEMS Microbiol. Lett.   306 ( 1 )   54 - 60   2010.05

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    Kind of work：Joint Work  

  • Development of a haemolysin gene-based multiplex PCR for simultaneous detection of Vibrio campbellii, Vibrio harveyi and Vibrio parahamolyticus Reviewed

    S. Haldar, S.B. Neogi, K. Kogure, S. Chatterjee, N. Chowdhury, A. Hinenoya, M. Asakura and S. Yamasaki

    Lett. Appl. Microbiol. Wiley-Blackwell   50 ( 2 )   146 - 152   2010.02

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    Kind of work：Joint Work  

  • Prevalence and characteristics of cytolethal distending toxin (Cdt)-producing Escherichia coli from children with diarrhea in Japan Reviewed

    A. Hinenoya, A. Naigita, K. Ninomiya, M. Okuda, K. Shima, M. Asakura, K. Nishimura, K. Seto, T. Tsukamoto, T. Ramamurthy and S. Yamasaki

    Microbiol. Immunol. Wiley-Blackwell   53 ( 4 )   206 - 215   2009.04

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    Kind of work：Single Work  

  • Rapid culture-free identification and molecular typing of Shiga toxin-producing Escherichia coli by PCR-RFLP Reviewed

    K. Shima, N. Kawamura, A. Hinenoya, N. Sugimoto, Y. Wu, M. Asakura, K. Nishimura, G.B. Nair and S. Yamasaki

    Microbiol. Immunol. Wiley-Blackwell   52 ( 6 )   310 - 313   2008.06

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    Kind of work：Joint Work  

  • Development of a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the detection and identification of Campylobacter jejuni, Campylobacter coli and Campylobacter fetus Reviewed

    M. Asakura, W. Samosornsuk, A. Hinenoya, N. Misawa, K. Nishimura, A. Matsuhisa and S. Yamasaki

    FEMS Immunol. Med. Microbiol. Federation of European Microbiological Societies   52 ( 2 )   260 - 266   2008.03

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    Kind of work：Joint Work  

  • An inducible lambdoid prophage encoding cytolethal distending toxin (Cdt-I) and a type III effector protein in enteropathogenic Escherichia coli Reviewed

    M. Asakura, A. Hinenoya, M. S. Alam, K. Shima, S. H. Zahid, L. Shi, N. Sugimoto, A. N. Ghosh, T. Ramamurthy, S. M. Faruque, G. B. Nair and S. Yamasaki

    Proc. Natl. Acad. Sci. USA National Academy of Sciences   104 ( 36 )   14483 - 14488   2007.09

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    Kind of work：Joint Work  

  • Cytolethal distending toxin (Cdt)-producing Escherichia coli isolated from a child with bloody diarrhea in Japan Reviewed

    A. Hinenoya, A. Nagita, M. Asakura, T. Tsukamoto, T. Ramamurthy, G. B. Nair, Y. Takeda and S. Yamasaki.

    Microbiol. Immunol. Wiley-Blackwell   51 ( 4 )   435 - 438   2007.04

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    Kind of work：Single Work  

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Books and Other Publications

  • ベトナムの養鶏場における薬剤耐性菌の現状

    日根野谷淳

    日本防菌防黴学会  2018.12 

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    Responsible for pages：551-560  

  • 消化管感染症の発症メカニズム（下痢原性大腸菌）

    山崎伸二, 日根野谷淳（ Role： Joint author）

    化学療法の領域  2016.09 

  • カンピロバクター感染症

    山崎伸二、日根野谷淳（ Role： Joint author）

    医学書院  2014.07 

  • Inhibition of virulence potential of Vibrio cholerae by natural compounds

    S Yamasaki, M. Asakura, S.B. Neogi, A Hinenoya, E Iwaoka, S Aoki（ Role： Joint author）

    Indian J Med Res  2011.02 

  • Molecular epidemiology of Vibrio cholerae and campylobacters isolated in Asian countries

    S Yamasaki, M Asakura, S Shiramaru, A Hinenoya, W Samosornsuk, L Shi, T Ramamurthy（ Role： Sole author）

    Springer  2010.02 

MISC

  • 新興人獣共通感染症細菌Escherichia albertiiに関する研究

    日根野谷 淳

    日本細菌学雑誌   76 ( 4 )   175 - 185   2021.11（ ISSN:0021-4930 ）

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    Escherichia albertiiは2003年に新たに命名された新興人獣共通腸管感染症細菌である。近年国内では,患者数100名を超える規模の集団食中毒事例が複数報告されている。本菌は腸管病原性大腸菌と同様,付着因子インチミンをコードするeae遺伝子を保有するが,一部の菌株が腸管出血性大腸菌(EHEC)の主要病原因子である志賀毒素2(stx2a,stx2f)遺伝子を保有することも明らかになったことから,注意を要する細菌であると言える。しかしながら,本菌の基本性状についての情報は乏しく,検査法も確立されていなかったため,本菌感染症の発生状況,感染源,感染経路など未だ不明な点が多い。著者は,継続的に行ってきた細胞膨化致死毒素産生大腸菌の分子疫学研究の中での偶然の発見がきっかけで本菌の研究を始めることとなった。これまでの研究では,本菌の検出,分離,同定を高精度に行える方法を構築し,この方法を用いて本菌の自然宿主の同定を目的とした保菌動物の調査を行ってきた。本稿では,これらの成果について紹介したい。(著者抄録)

  • [Molecular epidemiology of Escherichia albertii, emerging zoonotic enteropathogen]. International journal

    Atsushi Hinenoya

    Nihon saikingaku zasshi. Japanese journal of bacteriology   76 ( 4 )   175 - 185   2021（ ISSN:0021-4930 ）

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    Escherichia albertii is an emerging zoonotic enteric pathogen, closely related to E. coli. Several foodborne outbreaks caused by E. albertii accounting for >100 patients have recently occurred in Japan. This bacterium carries eae gene, similar to enteropathogenic E. coli. Some of them harbor Shiga toxin 2 (stx2a, stx2f) genes, primary virulence factor of enterohemorrhagic E. coli (EHEC), suggesting that the Stx2 producers could cause severe diseases such as HUS in humans. However, due to lack of the knowledges about its bacteriological characteristics and of the diagnostic methods, E. albertii-related infections might have been underestimated, and the infection sources and routes have not yet been understood. We had continuously performed molecular epidemiological studies targeting for cytolethal distending toxin-producing E. coli, and unexpectedly found that cdt-II gene-positive isolates were not E. coli but E. albertii. This finding led us to initiate research more focusing on E. albertii. We have constructed simple, efficient and reliable methods for the detection, isolation and identification of this bacterium by developing an E. albertii-specific PCR assay targeting Eacdt genes and E. albertii-selective isolation medium named XRM-MacConkey agar. We have also identified raccoons as a potential natural reservoir of E. albertii through wildlife survey using these methods. Here, I describe what I have studied with my colleagues.

    DOI： 10.3412/jsb.76.175

    PubMed

Presentations

  • 野生アライグマにおけるEscherichia albertiiの宿主内多様性(Within-host diversity of Escherichia albertii in wild raccoons)

    日根野谷 淳, 山崎 萌子, 徐 炳テイ, Awasthi Sharda, 畑中 律敏, 山崎 伸二

    日本細菌学雑誌  2023.02  日本細菌学会

  • Escherichia albertiiが原因と考えられる小児下痢症の2事例

    山崎 伸二, Awasthi Sharda Prasad, 竹平 京司, Okechukwu Obi John, 畑中 律敏, 日根野谷 淳, 名木田 章

    日本小児栄養消化器肝臓学会雑誌  2022.04  (一社)日本小児栄養消化器肝臓学会

  • 新興人獣共通感染症細菌Escherichia albertiiの検査法開発およびその応用 Domestic conference

    日根野谷淳

    第94回日本細菌学会総会  2021.03  日本細菌学会

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    Presentation type：Oral presentation (invited, special)  

  • Survey of Escherichia albertii in wild birds in Japan. Domestic conference

    A. Hinenoya, S.P. Awasthi, N. Yasuda, K. Nagano, J. Hassan, K. Takehira, N. Hatanaka, H. Inoue, S. Yamasaki

    第94回日本細菌学会総会  2021.03  日本細菌学会

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    Presentation type：Poster presentation  

  • 日本の野鳥を対象としたEscherichia albertiiの保菌調査(Survey of Escherichia albertii in wild birds in Japan)

    日根野谷 淳, Awasthi Sharda Prasad, 安田 憲朋, 長野 恵吾, Hassan Jayedul, 竹平 京司, 畑中 律敏, 井上 春奈, 山崎 伸二

    日本細菌学雑誌  2021.02  日本細菌学会

  • 新興腸管感染症起因菌Escherichia albertiiの検査法と新たに見えてきたこと Domestic conference

    日根野谷淳

    第93回日本細菌学会総会  2020.02  日本細菌学会

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    Presentation type：Poster presentation  

  • 分類・疫学・感染症　我が国における単相変異型Salmonella Typhimuriumの小進化

    新井 暢夫, 関塚 剛史, 玉村 雪乃, 楠本 正博, 日根野谷 淳, 山崎 伸二, 岩田 剛敏, 渡部 綾子, 黒田 誠, 秋庭 正人

    日本細菌学雑誌  2020.01  日本細菌学会

  • 話題の感染症　新興腸管感染症起因菌Escherichia albertiiの検査法と新たに見えてきたこと

    日根野谷 淳

    日本細菌学雑誌  2020.01  日本細菌学会

  • Development of detection, isolation and identification methods for Escherichia albertii. International conference

    A. Hinenoya, H. Ichimura, K. Nagano, S.P. Awasthi, N. Yasuda, K. Okuno, N. Hatanaka, S. Harada, K. Yamada, M. Suzuki, Y. Iijima, M.J. Albert, J. Yatsuyanagi, A. Nagita, S. Yamasaki.

    54th Annual Joint Panel Meeting on Cholera and Other Bacterial Enteric Infections.  2019.12 

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    Presentation type：Poster presentation  

  • 新興人獣共通感染症細菌Escherichia albertiiの自然宿主同定の試み Domestic conference

    日根野谷淳、長野恵吾、S.P. Awasthi、畑中律敏、山崎伸二

    第23回腸管出血性大腸菌感染症研究会  2019.11  腸管出血性大腸菌感染症研究会

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    Presentation type：Poster presentation  

  • 有機物存在下における亜塩素酸水と次亜塩素酸NaのCampylobacterに対する殺菌効果の比較

    畑中 律敏, Awasthi Sharda Prasad, 合田 学剛, 川田 宏之, 内野 譲, 久保 貴大, 日根野谷 淳, 山崎 伸二

    日本カンピロバクター研究会総会抄録集  2019.09  日本カンピロバクター研究会

  • Development and evaluation of a selective medium for isolation of Escherichia albertii. International conference

    A. Hinenoya, K. Nagano, S.P. Awasthi, N. Hatanaka, S. Yamasaki

    ASM microbe 2019  2019.06 

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    Presentation type：Poster presentation  

  • Escherichia albertiiを分離するための選択培地であるXRM-MacConkey寒天の開発(Development of XRM-MacConkey agar, a selective medium for isolation of Escherichia albertii)

    日根野谷 淳, 長野 恵吾, Awasthi Sharda, 畑中 律敏, 山崎 伸二

    日本細菌学雑誌  2019.03  日本細菌学会

  • Salmonella genomic island 3は宿主の重金属抵抗性を増強するintegrative and conjugative elementである

    新井 暢夫, 関塚 剛史, 玉村 雪乃, 楠本 正博, 日根野谷 淳, 山崎 伸二, 岩田 剛敏, 渡部 綾子, 黒田 誠, 秋庭 正人

    日本細菌学雑誌  2019.03  日本細菌学会

  • Development of XRM-MacConkey agar, a selective medium for isolation of Escherichia albertii. Domestic conference

    2019.02 

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    Presentation type：Poster presentation  

  • Retrospective surveillance of Escherichia albertii in the E. coli isolates from pet animals and poultry in the United States. International conference

    A. Hinenoya, X. Zeng, B. Gillespie, O. Sahin, C. Logue, S. Yamasaki, J. Lin

    ASM microbe 2018  2018.06 

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    Presentation type：Poster presentation  

  • Stx2f-producing Escherichia albertii infection causes mice death. International conference

    A. Hinenoya, Y. Shimomura, N. Yasuda, S.P. Awasthi, J. Yatsuyanagi, S. Yamasaki

    ASM microbe 2017  2017.06 

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    Presentation type：Poster presentation  

  • Evaluation of Stx2f-producing Escherichia albertii virulence in mice model. Domestic conference

    2017.01 

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    Presentation type：Poster presentation  

  • Stx2f産生Escherichia albertiiのマウスに対する病原性評価 Domestic conference

    日根野谷淳、下村義雄、安田憲朋、八柳潤、山崎伸二

    第20回腸管出血性大腸菌感染症研究会  2016.11  腸管出血性大腸菌感染症研究会

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    Presentation type：Poster presentation  

  • Phenotypic analysis of CDT-I phages in Escherichia coli. Domestic conference

    A. Hinenoya, M. Asakura, S. Yamasaki

    88th general meeting of Japanese Society for Microbiology  2015.03 

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    Presentation type：Poster presentation  

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Industrial Property Rights

  • 選択分離用培地

    山崎伸二、日根野谷淳

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    property_type：Patent 

  • 消毒された肝臓の製造方法

    山崎伸二、日根野谷淳、森河内巌、山口葵、櫻本行利、西田和正、朝倉昌博

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    property_type：Patent 

    Patent/Registration no：6087915 

Grant-in-Aid for Scientific Research

  • 新興人獣共通感染症細菌Escherichia albertiiの環境内動態における水環境の重要性評価

    Grant-in-Aid for Scientific Research(C)  2026

  • 新興食中毒細菌Escherichia albertiiにおける新規分散付着因子の同定と性状解析

    Grant-in-Aid for Scientific Research(C)  2026

  • 新興人獣共通感染症細菌Escherichia albertiiの環境内動態における水環境の重要性評価

    Grant-in-Aid for Scientific Research(C)  2025

  • 新興食中毒細菌Escherichia albertiiにおける新規分散付着因子の同定と性状解析

    Grant-in-Aid for Scientific Research(C)  2025

  • 新興人獣共通感染症細菌Escherichia albertiiの環境内動態における水環境の重要性評価

    Grant-in-Aid for Scientific Research(C)  2024

  • 新興食中毒細菌Escherichia albertiiにおける新規分散付着因子の同定と性状解析

    Grant-in-Aid for Scientific Research(C)  2024

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Charge of on-campus class subject

  • 食品衛生検査学実習

    2024   Weekly class   Undergraduate

  • 獣医伝染病学B

    2024   Weekly class   Undergraduate

  • 獣医伝染病学A

    2024   Weekly class   Undergraduate

  • 人獣共通感染症学

    2024   Weekly class   Undergraduate

  • 獣医細菌学

    2024   Intensive lecture   Undergraduate

  • 獣医細菌学

    2024   Weekly class   Undergraduate

  • 小動物基礎臨床実習

    2024   Intensive lecture   Undergraduate

  • 獣医感染制御学

    2024   Weekly class   Undergraduate

  • 獣医科学英語演習

    2024   Intensive lecture   Undergraduate

  • 獣医微生物・免疫学実習

    2024   Weekly class   Undergraduate

  • 獣医伝染病学B

    2024   Weekly class   Undergraduate

  • 獣医微生物・免疫学実習

    2024   Weekly class   Undergraduate

  • 獣医体験演習

    2024   Weekly class   Undergraduate

  • 臨床基礎実習

    2024   Weekly class   Undergraduate

  • 分子細菌学特論

    2024   Intensive lecture   Graduate school

  • 感染症制御学特別講義C

    2024   Intensive lecture   Graduate school

  • 臨床基礎実習

    2024   Weekly class   Undergraduate

  • 獣医感染制御学実習

    2024   Intensive lecture   Undergraduate

  • 食品衛生検査学実習

    2024   Weekly class   Undergraduate

  • 獣医国際防疫学

    2024   Weekly class   Undergraduate

  • 国際食料流通演習

    2024   Intensive lecture   Undergraduate

  • 国際食料流通論

    2024   Intensive lecture   Undergraduate

  • Special Lecture: Infectious Diseases Control C

    2021    

  • Exercise in International Food Circulation

    2021   Practical Training  

  • Practice in Food Hygiene

    2021   Practical Training  

  • Practice in Veterinary Infectious Disease Control

    2021   Practical Training  

  • Practice in Analysis on Food Sanitation and Hygiene

    2021   Practical Training  

  • Topics in Life, Environment, and Advanced Sciences

    2021    

  • Infectious Diseases of Animals B

    2021    

  • Practice in Veterinary Microbiology and Immunology

    2021   Practical Training  

  • Veterinary Bacteriology

    2021    

  • Basic clinical practices

    2021   Practical Training  

  • Veterinary Infectious Disease Control

    2021    

  • Veterinary International Prevention of Epidemics

    2021    

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