Updated on 2024/06/20

写真a

 
Yasugi Mayo
 
Organization
Graduate School of Veterinary Science Department of Veterinary Science Associate Professor
School of Veterinary Science Department of Veterinary Science
Title
Associate Professor
Affiliation
Institute of Veterinary Science

Position

  • Graduate School of Veterinary Science Department of Veterinary Science 

    Associate Professor  2022.04 - Now

  • School of Veterinary Science Department of Veterinary Science 

    Associate Professor  2022.04 - Now

Degree

  • 博士（医学） （ Others ）

Research Areas

  • Life Science / Hygiene and public health (laboratory)

Research Interests

  • 人獣共通感染症

  • 細菌

  • ウイルス

  • ワンヘルス

Research subject summary

  • 人獣共通感染症の基礎研究

Research Career

  • 人獣共通ウイルス感染症の診断・予防法開発

    2019.01 - Now 

  • 薬剤耐性感染症のサーベイランス

    2018.01 - Now 

Professional Memberships

  • 日本感染症学会

      Domestic

  • 日本獣医師会

  • 日本細菌学会

  • 日本ウイルス学会

  • 日本獣医学会

Committee Memberships (off-campus)

  • 評議委員   日本獣医学会  

    2023.09 - 2025.09 

  • 獣医事審議会専門委員   獣医事審議会  

    2022.06 - 2025.06 

  • 運営委員   大阪府狂犬病等感染症対策委員会  

    2022.04 - Now 

  • 運営委員   近畿連合地区獣医学会運営委員会  

    2015.04 - Now 

Awards

  • 獣医学術近畿地区学会優秀研究者褒賞

    2019.10   近畿地区連合獣医師会  

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    Country：Japan

  • 大阪府獣医師会会長賞

    2019.05   公益社団法人大阪府獣医師会  

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    Country：Japan

  • 大阪府獣医師会賞

    2000.03   社団法人大阪府獣医師会  

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    Country：Japan

Papers

  • Characteristics of epitope dominance pattern and cross-variant neutralisation in 16 SARS-CoV-2 mRNA vaccine sera.

    Mayo Yasugi, Yu Nakagama, Natsuko Kaku, Yuko Nitahara, Noritoshi Hatanaka, Shinji Yamasaki, Yasutoshi Kido

    Vaccine   41 ( 42 )   6248 - 6254   2023.09（ ISSN:0264410X ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    SARS-CoV-2 serological studies suggest that individual serum antibody repertoires can affect neutralisation breadth. Herein, we asked whether a BNT162b2 vaccine-induced epitope dominance pattern (i.e., predominant viral structural domain targeted by serum antibodies for virus neutralisation) affects cross-variant neutralisation. When a neutralisation assay against the ancestral strain was carried out using 16 vaccine sera preabsorbed with a recombinant receptor-binding domain (RBD) or an N-terminal domain (NTD) protein, three and 13 sera, respectively, showed lower neutralisation under NTD and RBD protein-preabsorbed conditions than under the other protein-preabsorbed conditions. This suggests that the NTD was responsible for virus neutralisation in three sera, whereas the other 13 sera elicited RBD-dominant neutralisation. The results also suggest the presence of infectivity-enhancing antibodies in four out of the 13 RBD-dominant sera. A neutralisation assay using SARS-CoV-2 variants revealed that NTD-dominant sera showed significantly reduced neutralising activity against the B.1.617.2 variant, whereas RBD-dominant sera retained neutralising activity even in the presence of infectivity-enhancing antibodies. Taken together, these results suggest the followings: (i) epitope dominance patterns are divided into at least two types: NTD-dominant and RBD-dominant; (ii) NTD-dominant sera have less potential to neutralise the B.1.617.2 variant than RBD-dominant sera; and (iii) infectivity-enhancing antibodies play a limited role in cross-variant neutralisation against the five variants tested.

    DOI： 10.1016/j.vaccine.2023.08.076

    PubMed

  • Nsp14 of SARS-CoV-2 inhibits mRNA processing and nuclear export by targeting the nuclear cap-binding complex.

    Katahira J, Ohmae T, Yasugi M, Sasaki R, Itoh Y, Kohda T, Hieda M, Yokota Hirai M, Okamoto T, Miyamoto Y

    Nucleic acids research   51 ( 14 )   7602 - 7618   2023.06（ ISSN:0305-1048 ）

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  • Antiviral activity of curcumin and its analogs selected by an artificial intelligence-supported activity prediction system in SARS-CoV-2-infected VeroE6 cells

    Teshima K.

    Natural Product Research   1 - 6   2023（ ISSN:14786419 ）

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  • Genetic and phenotypic analyses of mcr-harboring extended-spectrum β-lactamase-producing Escherichia coli isolates from companion dogs and cats in Japan

    Mayo Yasugi, Shingo Hatoya, Daisuke Motooka, Daisuke Kondo, Hideo Akiyoshi, Masayuki Horie, Shota Nakamura, Terumasa Shimada

    Veterinary Microbiology   280   109695 - 109695   2023（ ISSN:0378-1135 ） （ eISSN:1873-2542 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    The emergence of mcr plasmid-mediated colistin-resistant extended-spectrum β-lactamase (ESBL)-producing Enterobacterales among companion dogs and cats poses a risk of the animals acting as reservoirs for cross-species transmission. However, current knowledge of mcr-harboring ESBL-producing Enterobacterales in companion dogs and cats is still limited; thus, the genetic and phenotypic characteristics of the bacterial isolates and plasmids, in companion dogs and cats, remain to be elucidated. Here, we identified mcr gene-harboring ESBL-producing Escherichia coli isolates during whole-genome sequencing of ESBL-producing E. coli isolates from a dog and a cat in Osaka, Japan. Colistin-resistant MY732 isolate from a dog carried two plasmids: mcr-1.1-harboring IncI2 plasmid and blaCTX-M-14-harboring IncFIB plasmid. Conjugation assays revealed that both plasmids can be co-transferred even though the IncFIB plasmid lacked a conjugal transfer gene cassette. The other isolate MY504 from a cat harbored two bla genes and mcr-9 on the identical IncHI2 plasmid. This isolate was not resistant to colistin, which is likely to be due to deletion of the regulatory two-component QseBC system associated with the mcr-9 expression. To the best of our knowledge, this is the first report of a colistin-resistant ESBL-producing E. coli isolate harboring mcr-1 from a companion dog in Japan. Given that the mcr gene-harboring IncI2 and IncHI2 plasmids in this study shared high homology with plasmids from human or animal-derived Enterobacterales, companion dogs and cats may act as important reservoirs for cross-species transmission of the mcr gene in the community, in Japan.

    DOI： 10.1016/j.vetmic.2023.109695

    PubMed

  • Antibody avidity maturation, following recovery from infection or the booster vaccination, grants breadth in SARS-CoV-2 neutralizing capacity. Reviewed

    Yu Nakagama, Katherine Candray, Natsuko Kaku, Yuko Komase, Maria-Virginia Rodriguez-Funes, Rhina Dominguez, Tomoya Tsuchida, Hiroyuki Kunishima, Etsuko Nagai, Eisuke Adachi, Dieudonné Mumba Ngoyi, Mari Yamasue, Kosaku Komiya, Kazufumi Hiramatsu, Naoto Uemura, Yuki Sugiura, Mayo Yasugi, Yuka Yamagishi, Hiroshige Mikamo, Satoshi Shiraishi, Takehiro Izumo, Sachie Nakagama, Chihiro Watanabe, Yuko Nitahara, Evariste Tshibangu-Kabamba, Hiroshi Kakeya, Yasutoshi Kido

    The Journal of infectious diseases   227 ( 6 )   780 - 787   2022（ ISSN:0022-1899 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    BACKGROUND: Cross-neutralizing capacity of antibodies against SARS-CoV-2 variants is important in mitigating (re-)exposures. Role of antibody maturation, the process whereby selection of higher affinity antibodies augments host immunity, to determine SARS-CoV-2 neutralizing capacity was investigated. METHODS: Sera from SARS-CoV-2 convalescents at 2-, 6-, or 10-months post-recovery, and BNT162b2 vaccine recipients at 3- or 25-weeks post-vaccination, were analyzed. Anti-spike IgG avidity was measured on urea-treated ELISAs. Neutralizing capacity was assessed by surrogate neutralization assays. Fold change between variant and wild-type neutralization inferred the breadth of neutralizing capacity. RESULTS: Compared with early-convalescence, avidity indices of late-convalescent sera were significantly higher (median 37.7 (interquartile range 28.4-45.1) vs. 64.9 (57.5-71.5), p < 0.0001). Urea-resistant, high-avidity IgG best predicted neutralizing capacity (Spearman's r = 0.49 vs. 0.67 (wild-type); 0.18-0.52 vs. 0.48-0.83 (variants)). Higher-avidity convalescent sera better cross-neutralized SARS-CoV-2 variants (p < 0.001 (Alpha); p < 0.01 (Delta and Omicron)). Vaccinees only experienced meaningful avidity maturation following the booster dose, exhibiting rather limited cross-neutralizing capacity at week-25. CONCLUSIONS: Avidity maturation was progressive beyond acute recovery from infection, or became apparent after the booster vaccine dose, granting broader anti-SARS-CoV-2 neutralizing capacity. Understanding the maturation kinetics of the two building blocks of anti-SARS-CoV-2 humoral immunity is crucial.

    DOI： 10.1093/infdis/jiac492

    PubMed

  • Tea crude extracts effectively inactivate severe acute respiratory syndrome coronavirus 2

    Ishimoto, K., Hatanaka, N., Otani, S., Maeda, S., Xu, B., Yasugi, M., Moore, J.E., Suzuki, M., Nakagawa, S., Yamasaki, S.

    Letters in Applied Microbiology   74 ( 1 )   2022（ ISSN:1472-765X ） （ eISSN:1472-765X ）

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    Publishing type：Research paper (scientific journal)  

    It is well known that black and green tea extracts, particularly polyphenols, have antimicrobial activity against various pathogenic microbes including viruses. However, there is limited data on the antiviral activity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which emerged rapidly in China in late 2019 and which has been responsible for coronavirus disease 2019 (COVID-19) pandemic globally. In this study, 20 compounds and three extracts were obtained from black and green tea and found that three tea extracts showed significant antiviral activity against SARS-CoV-2, whereby the viral titre decreased about 5 logs TCID50 per ml by 1·375 mg ml−1 black tea extract and two-fold diluted tea bag infusion obtained from black tea when incubated at 25°C for 10 s. However, when concentrations of black and green tea extracts were equally adjusted to 344 µg ml−1, green tea extracts showed more antiviral activity against SARS-CoV-2. This simple and highly respected beverage may be a cheap and widely acceptable means to reduce SARS-CoV-2 viral burden in the mouth and upper gastrointestinal and respiratory tracts in developed as well as developing countries.

    DOI： 10.1111/lam.13591

  • ShellCoat, a Calcinated Calcium Solution, Effectively Inactivates SARS-CoV-2.

    Noritoshi Hatanaka, Bingting Xu, Yasuharu Yamashita, Hiroo Kawakami, Mayo Yasugi, Shinji Yamasaki

    Biocontrol science   27 ( 1 )   53 - 56   2022（ ISSN:1884-0205 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：Domestic journal  

    SARS-CoV-2, an acute respiratory syndrome-causing virus, suddenly emerged at the end of 2019 in China, and rapidly spread all over the world. In this study, we examined whether a calcinated calcium solution (ShellCoat) , which has been approved as a food additive in Japan can inactivate SARS-CoV-2. Furthermore, antiviral activity of ShellCoat against SARS-CoV-2 was also evaluated in the presence of organic matter, namely, fetal bovine serum (FBS) . When concentrated SARS-CoV-2 were treated with ShellCoat for 10 sec in presence or absence of FBS as organic matters, the viral titer was decreased more than 4 logs 50% tissue culture infective dose per mL (TCID50/mL) but use of ShellCoat for 20 sec or more under similar experimental conditions the viral titer was below the detection limit (≦2.1 logs TCID50/mL) . These results clearly indicate that the ShellCoat is a powerful antiviral agent against SARS-CoV-2 even in the presence of organic matters.

    DOI： 10.4265/bio.27.53

    PubMed

  • Mechanisms underlying inactivation of SARS-CoV-2 by nano-sized electrostatic atomized water particles

    Yasugi, M., Komura, Y., Ishigami, Y.

    Journal of Nanoparticle Research   24 ( 5 )   99   2022（ ISSN:1572-896X ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s11051-022-05485-5

    PubMed

  • Isolation and characterization of Staphylococcus argenteus strains from retail foods and slaughterhouses in Japan.

    Yuki Wakabayashi, Kohei Takemoto, Satomi Iwasaki, Tomoya Yajima, Akiko Kido, Akiko Yamauchi, Kyoko Kuroiwa, Yuko Kumai, Shizue Yoshihara, Hayato Tokumoto, Kentaro Kawatsu, Mayo Yasugi, Masami Miyake

    International journal of food microbiology   363   109503   2022（ ISSN:1879-3460 ）

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    International / domestic magazine：International journal  

    Staphylococcus argenteus has been recently established as a novel species of Staphylococcus aureus complex. It is known to cause various human diseases, such as skin and soft-tissue infections, sepsis, and staphylococcal food poisoning, although the source of infection has not been clearly described. In food poisoning cases, the source of bacterial contamination in food is unknown. This study examined the prevalence of S. argenteus among retail fresh food and poultry slaughterhouses in Japan. Among 642 food samples examined, successful isolation of S. argenteus was achieved in 21 of 151 (13.9%) chicken samples. No isolations from pork, beef, fish, or vegetables in retail markets were confirmed. Multiple-locus sequence typing revealed that the 21 isolates were classified into four sequence types (ST) that were divided into 14 subtypes using spa-typing. All food isolates were susceptible to methicillin and did not show positivity for the Panton-Valentine leukocidin gene. When bacteria were isolated from two poultry slaughterhouses in the same region, 14 S. argenteus strains were successfully isolated from only one slaughterhouse. Thirteen of 14 strains were isolated from a poultry carcass and slaughterhouse environments during a certain sampling period and were all classified as ST5961 with identical spa-type. Also, the number of single nucleotide variants (SNVs) detected on the core genomes of the same 13 strains were between 0 and 17, suggesting a long-term inhabitation of an S. argenteus strain inside the facility. Furthermore, one isolate from chicken meat was also genetically linked with the same lineage of slaughterhouse isolates, with ≤15 SNVs being detected. Additionally, one slaughterhouse isolate from chiller water and three chicken isolates were classified into the same cluster by phylogenetic analysis, although the number of pairwise SNVs ranged from 62 to 128. To the best of the authors' knowledge, this is the first study that demonstrated S. argenteus in a food processing facility and the possible bacterial contamination on food during food processing.

    DOI： 10.1016/j.ijfoodmicro.2021.109503

    PubMed

  • Identification of SARS-CoV-2 main protease inhibitors from FDA-approved drugs by artificial intelligence-supported activity prediction system

    Komatsu, H., Tanaka, T., Ye, Z., Ikeda, K., Matsuzaki, T., Yasugi, M., Hosoda, M.

    Journal of Biomolecular Structure and Dynamics   2022（ ISSN:1538-0254 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1080/07391102.2021.2024260

  • Comparative evaluation of chlorous acid and sodium hypochlorite activity against SARS-CoV-2.

    Noritoshi Hatanaka, Sharda Prasad Awasthi, Bingting Xu, Hisataka Goda, Hiroyuki Kawata, Isanori Horiuchi, Mayo Yasugi, Shinji Yamasaki

    Access microbiology   4 ( 5 )   acmi000354   2022

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    International / domestic magazine：International journal  

    A novel coronavirus, named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), suddenly emerged in China in 2019, spread globally and caused the present COVID-19 pandemic. Therefore, to mitigate SARS-CoV-2 infection effective measures are essential. Chlorous acid (HClO2) has been shown to be an effective antimicrobial agent. However, at present there is no experimental evidence showing that HClO2 can inactivate SARS-CoV-2. Therefore, in this study, we examined the potential of HClO2 to inactivate SARS-CoV-2 in presence or absence of organic matter and the results were compared with that of sodium hypochlorite (NaClO), another potent antimicrobial agent. When concentrated SARS-CoV-2 was incubated with 10 ppm HClO2 for 10 s, viral titre was decreased by 5 log of 50% tissue culture infective dose per mL (TCID50 ml-1). However, the same concentration of NaClO could not inactivate SARS-CoV-2 as effectively as HClO2 did even after incubation for 3 min. Furthermore, 10 ppm HClO2 also inactivated more than 4.0 log of TCID50 within 10 s in the presence of 5 % fetal bovine serum used as mixed organic matters. Our results obtained with HClO2 are more effective against SARS-CoV-2 as compared to NaClO that can be used for disinfectant against SARS-CoV-2 .

    DOI： 10.1099/acmi.0.000354

    PubMed

  • 低濃度の有機物存在下におけるオゾン水の新型コロナウイルス(SARS-CoV-2)に対する不活化効果

    安木 真世, 黒田 真未, 井 千尋

    室内環境   24 ( 1 )   13 - 18   2021.04（ ISSN:1882-0395 ）

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    世界的流行が続く新型コロナウイルス感染症の原因ウイルスSARS-CoV-2の不活化技術として,アルコールや界面活性剤に加え,オゾン水の有効性が報告された。有機物の存在はオゾンの分解を速めて有効オゾン濃度の減衰を招くことが知られており,ウイルスが存在し得る様々な環境を想定してオゾン水の有効性を評価すること,更には様々な環境に応じてオゾン水の使用条件を設定することが重要な課題である。本研究ではこれら課題のための基盤実験として,有機物の影響を抑えた(ウイルス培養液の溶媒をリン酸緩衝液に置換した)条件におけるSARS-CoV-2に対するオゾン水の不活化効果を評価した。その結果,(1)タンパク質濃度3.3μg/mlのウイルス溶液では,0.1mg/l濃度のオゾン水の30秒間曝露で生残ウイルス量が1000分の1に,(2)タンパク質濃度33μg/mlのウイルス溶液では,0.5mg/l濃度のオゾン水の3分間曝露で生残ウイルス量が10分の1に減少し,低濃度のオゾン水がSARS-CoV-2を有意に不活化することが明らかとなった。(著者抄録)

  • Discrepant antigen-specific antibody responses causing SARS-CoV-2 persistence in a patient receiving B-cell-targeted therapy with rituximab Reviewed

    Takakuwa T, Nakagama Y, Yasugi M, Maeda T, Matsuno K, Kiritoshi A, Deguchi R, Hagawa N, Shibata W, Oshima K, Yamamoto K, Uchida K, Noda T, Yamada K, Nishimura T, Yamamoto H, Kido Y, Hino M, Kakeya H, Mizobata Y

    60   2021

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    Kind of work：Joint Work  

  • High-resolution linear epitope mapping of the receptor binding domain of SARS-CoV-2 spike protein in COVID-19 mRNA vaccine recipients Reviewed

    Nitahara Y, Nakagama Y, Kaku N, Candray K, Michimuko Y, Tshibangu-Kabamba E, Kaneko A, Yamamoto H, Mizobata Y, Kakeya H, Yasugi M, Kido Y

    9 ( 3 )   e00965 - 21   2021

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    Kind of work：Joint Work  

  • Hypochlorous acid solution is a potent antiviral agent against SARS-CoV-2 Reviewed

    Hatanaka N, Yasugi M, Sato T, Mukamoto M, Yamasaki S

    2021

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    Kind of work：Joint Work  

  • Chlorine dioxide is a more potent antiviral agent against SARS-CoV-2 than sodium hypochlorite

    Hatanaka, N., Xu, B., Yasugi, M., Morino, H., Tagishi, H., Miura, T., Shibata, T., Yamasaki, S.

    Journal of Hospital Infection   118   20 - 26   2021（ ISSN:1532-2939 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jhin.2021.09.006

  • 低濃度エタノールと界面活性剤の併用による新型コロナウイルスに対する不活化効果

    國武 広一郎, 村上 拡, 佃 一訓, 小崎 俊司, 安木 真世

    日本食品微生物学会雑誌   38 ( 2 )   93 - 97   2021（ ISSN:1340-8267 ）

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    食にかかわる環境におけるSARS-CoV-2に対する最適な消毒剤として、SARS-CoV-2に対し有効な消毒剤であるエタノールに着目し、高濃度で使用するためコスト高になる、パーティションとして使われるアクリルなど一部のプラスチックには使用できないなどのエタノールのデメリットを、エタノールを低濃度にすることで解消し、SARS-CoV-2に対して不活化効果が認められている界面活性剤を配合することで、ウイルスや細菌に対する不活化効果と洗浄効果を兼ね備えた硬質表面用細菌洗浄剤を開発した。その結果、10%エタノールと3種の界面活性剤の組み合わせがSARS-CoV-2ウイルスへの不活化効果を有することが明らかになり、外食産業などにおけるアクリル板などのパーティションをはじめ、フェイスガードや店内設備(テーブル、イス、ドアノブ、タッチパネルなど)の清拭など、幅広い使用が可能であると考えられた。

  • Whole-genome analyses of extended-spectrum or AmpC β-lactamase-producing Escherichia coli isolates from companion dogs in Japan.

    Mayo Yasugi, Shingo Hatoya, Daisuke Motooka, Yuki Matsumoto, Shunsuke Shimamura, Hiroyuki Tani, Masaru Furuya, Keiichiro Mie, Masami Miyake, Shota Nakamura, Terumasa Shimada

    PloS one   16 ( 2 )   e0246482   2021

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    The emergence and global spread of extended-spectrum or AmpC β-lactamase (ESBL/AmpC)-producing Enterobacteriaceae in companion animals have led to the hypothesis that companion animals might be reservoirs for cross-species transmission because of their close contact with humans. However, current knowledge in this field is limited; therefore, the role of companion animals in cross-species transmission remains to be elucidated. Herein, we studied ESBL/AmpC-producing Enterobacteriaceae, Escherichia coli in particular, isolated from extraintestinal sites and feces of companion dogs. Whole-genome sequencing analysis revealed that (i) extraintestinal E. coli isolates were most closely related to those isolated from feces from the same dog, (ii) chromosomal sequences in the ST131/C1-M27 clade isolated from companion dogs were highly similar to those in the ST131/C1-M27 clade of human origin, (iii) certain plasmids, such as IncFII/pMLST F1:A2:B20/blaCTX-M-27, IncI1/pMLST16/blaCTX-M-15, or IncI1/blaCMY-2 from dog-derived E. coli isolates, shared high homology with those from several human-derived Enterobacteriaceae, (iv) chromosomal blaCTX-M-14 was identified in the ST38 isolate from a companion dog, and (v) eight out of 14 tested ESBL/AmpC-producing E. coli isolates (i.e., ST131, ST68, ST405, and ST998) belonged to the human extraintestinal pathogenic E. coli (ExPEC) group. All of the bla-coding plasmids that were sequenced genome-wide were capable of horizontal transfer. These results suggest that companion dogs can spread ESBL/AmpC-producing ExPEC via their feces. Furthermore, at least some ESBL/AmpC-producing ExPECs and bla-coding plasmids can be transmitted between humans and companion dogs. Thus, companion dogs can act as an important reservoir for ESBL/AmpC-producing E. coli in the community.

    DOI： 10.1371/journal.pone.0246482

    PubMed

  • 帯電微粒子水の新型コロナウイルス(SARS-CoV-2)に対する不活化効果

    安木 真世, 小村 泰浩, 石上 陽平

    室内環境   23 ( 3 )   241 - 246   2020.12（ ISSN:1882-0395 ）

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    2019年に発生した新型コロナウイルス感染症は世界的大流行を引き起こした。本ウイルスに対するワクチンの実用化が待たれる中,感染予防対策として環境中のウイルスの不活化技術や除去技術に注目が集まっている。静電霧化装置から発生するナノサイズの帯電微粒子水は様々な細菌を不活化することが報告されている。本研究では新型コロナウイルスに対する帯電微粒子水の効果を評価した。帯電微粒子水に曝露された新型コロナウイルスは時間経過とともに有意に不活化され,3時間後の生残ウイルスは対照群と比較して1000分の1であった。以上の結果から,帯電微粒子水は新型コロナウイルスの不活化に有効であることが明らかとなった。(著者抄録)

  • Clinical findings and treatment of disseminated Mycobacterium avium subspecies hominissuis infection in a domestic cat Reviewed

    Kanegi R, Yasugi M, Nabetani T, Tanaka T, Wada Y, Hirai K, Sugiura K, Hatoya S

    81 ( 12 )   2019

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    Kind of work：Joint Work  

  • An enhanced green fluorescence protein (EGFP)-based reporter assay for quantitative detection of sporulation in Clostridium perfringens SM101 Reviewed

    Wakabayashi Y, Nariya H, Yasugi M, Kuwahara T, Sarker MR, Miyake M

    2019

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    Kind of work：Joint Work  

  • Phosphorothioation of foreign DNA influences the transformation efficiency in Clostridium perfringens NCTC8239 Reviewed

    Yasugi, M., Motooka, D., Nakamura, S., Miyake, M.

    Anaerobe   61   102085   2019（ ISSN:1075-9964 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.anaerobe.2019.102085

    PubMed

  • Effect of sublethal heat treatment on the later stage of germination-to-outgrowth of Clostridium perfringens spores Reviewed

    Sakanoue H, Yasugi M, Miyake M

    Microbiology and Immunology 雑誌   62   2018.06

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    Kind of work：Joint Work  

  • Spores of anaerobic bacteria: characteristics ad behaviors during restoration from damaged status Reviewed

    三宅眞実、幸田知子、安木真世、坂野上英世、平田祥太郎

    日本食品科学工学会誌 雑誌   65   2018.03

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    Kind of work：Joint Work  

  • Adherence of Clostridium perfringens spores to human intestinal epithelial Caco-2 cells Reviewed

    Sakanoue H, Nakano T, Sano K, Yasugi M, Monma C, Miyake M

    FEMS microbiology letters 雑誌   365   2018.03

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    Kind of work：Joint Work  

  • Nitrate salts suppress sporulation and production of enterotoxin in Clostridium perfringens strain NCTC8239 Reviewed

    Yasugi M, Otsuka K, Miyake M

    Microbiology and Immunology 雑誌   2016.10

    　More details

    Kind of work：Joint Work  

  • Recombinant production and characterization of human anti-influenza virus monoclonal antibodies identified from hybridomas fused with human lymphocytes Reviewed

    Misaki R, Fukura N, Kajiura H, Yasugi M, Kubota-Koketsu R, Sasaki T, Momota M, Ono K, Ohashi T, Ikuta K, Fujiyama K

    Biologicals 雑誌   44   2016.09

    　More details

    Kind of work：Joint Work  

  • Transcriptional profile during deoxycholate-induced sporulation in a Clostridium perfringens isolate causing foodborne illness. Reviewed

    Yasugi M, Okuzaki D, Kuwana R, Takmatsu H, Fujita M, Sarker MR, Miyake M

    Applied and Environmental Microbiology 雑誌   82   2016.03

    　More details

    Kind of work：Joint Work  

  • Influenza A virus-induced expression of a GalNAc transferase, GALNT3, via microRNAs is required for enhanced viral replication Reviewed

    Nakamura S, Horie M, Daidoji T, Honda T, Yasugi M, Kono A, Komori T, Okuzaki D, Narimatsu H, Nakaya T, Tomonaga K

    Journal of Virology 雑誌   90   2015.12

    　More details

    Kind of work：Joint Work  

  • Avian influenza virus infection of immortalized human respiratory epithelial cells depends upon a delicate balance between hemagglutinin acid stability and endosomal pH Reviewed

    Tomo Daidoji, Yohei Watanabe, Madiha S. Ibrahim, Mayo Yasugi, Hisataka Maruyama, Taisuke Masuda, Fumihito Arai, Tomoyuki Ohba, Ayae Honda, Kazuyoshi Ikuta, Takaaki Nakaya

    Journal of biological chemistry 雑誌   290 ( 17 )   2015.04

    　More details

    Kind of work：Joint Work  

  • In vitro cytotoxicity induced by Clostridium perfringens isolate carrying a chromosomal cpe gene is exclusively dependent on sporulation and enterotoxin production Reviewed

    Mayo Yasugi, Yuki Sugahara, Hidenobu Hoshi, Kaori Kondo, Prabhat K Talukdar, Mahfuzur R Sarker, Shigeki Yamamoto, Yoichi Kamata, Masami Miyake

    Microbial pathogenesis 雑誌   85   2015.04

    　More details

    Kind of work：Joint Work  

  • A human monoclonal antibody derived from a vaccinated volunteer recognizes heterosubtypically a novel epitope on the hemagglutinin globular head of H1 and H9 influenza A viruses Reviewed

    Boonsathorn N, Panthong S, Koksunan S, Chittaganpitch M, Phuygun S, Waicharoen S, Prachasupap A, Sasaki T, Kubota-Koketsu R, Yasugi M, Ono K, Arai Y, Kurosu T, Sawanpanyalert P, Ikuta K, Watanabe Y

    Biochemical and Biophysical Research Communications 雑誌   452   2014.09

    　More details

    Kind of work：Joint Work  

  • Human monoclonal antibodies derived from a patient infected with 2009 pandemic influenza A virus broadly cross-neutralize group 1 influenza viruses Reviewed

    Y. Pang, T. Sasaki, R. Kubota-Koketsu, Y. Inoue, M. Yasugi, A. Yamashita, R. Ramadhany, Y. Arai, A. Du, N. Boonsathorn, M.S. Ibrahim, T. Daidoji, T. Nakaya, K. Ono, Y. Okuno, K. Ikuta, Y. Watanabe

    Biochemical and Biophysical Research Communications 雑誌   450   2014.05

    　More details

    Kind of work：Joint Work  

  • Emerging Antigenic Variants at the Antigenic Site Sb in Pandemic A(H1N1)2009 Influenza Virus in Japan Detected by a Human Monoclonal Antibody Reviewed

    Mayo Yasugi, Ritsuko Kubota-Koketsu, Akifumi Yamashita, Norihito Kawashita, Anariwa Du, Ryo Misaki, Motoki Kuhara, Paphatsawan Boonsathorn, Kazuhito Fujiyama, Yoshinobu Okuno, Takaaki Nakaya, Kazuyoshi Ikuta

    PLoS ONE   8   2013.10

  • Human monoclonal Antibodies Broadly Neutralizing against Influenza B Virus

    Mayo Yasugi, Ritsuko Kubota-Koketsu, Akifumi Yamashita, Norihito Kawashita, Anariwa Du, Tadahiro Sasaki, Mitsuhiro Nishimura, Ryo Misaki, Motoki Kuhara, Naphatsawan Boonsathorn, Kazuhito Fujiyama, Yoshinobu Okuno, Takaaki Nakaya, Kazuyoshi Ikuta

    PLoS Pathogens   9 ( 2 )   2013.02

  • Tropism of pandemic 2009 H1N1 influenza A virus

    Ririn Ramadhany, Mayo Yasugi, Shota Nakamura, Tomo Daidoji, Yohei Watanabe, Kazuo Takahashi, Kazuyoshi Ikuta, Takaaki Nakaya

    Frontiers in Microbiology   3   2012.04

  • Frequency of D222G and Q223R Hemagglutinin Mutants of Pandemic (H1N1) 2009 Influenza Virus in Japan between 2009 and 2010

    Mayo Yasugi, Shota Nakamura, Tomo Daidoji, Norihito Kawashita, Ririn Ramadhany, Cheng-Song Yang, Teruo Yasunaga, Tetsuya Iida, Toshihiro Horii, Kazuyoshi Ikuta, Kazuo Takahashi, Takaaki Nakaya

    PLoS ONE   7 ( 2 )   2012.02

  • Highly Pathogenic H5N1 Avian Influenza Virus Induces Extracellular Ca2+ Influx, Leading to Apoptosis in Avian Cells

    Mayo Ueda, Tomo Daidoji, Anariwa Du, Cheng-Song Yang, Madiha S. Ibrahim, Kazuyoshi Ikuta, Takaaki Nakaya

    Journal of Virology   84 ( 6 )   3068 - 3078   2010.03

  • Direct metagenomic detection of Viral Pathogens in Nasal and Fecal Specimens Using an Unbiased High-Throughput Sequencing Approach

    Shota Nakamura, Cheng-Song Yang, Naomi Sakon, Mayo Ueda, Takahiro Tougan, Akifumi Yamashita, Naohisa Goto, Kazuo Takahashi, Teruo Yasunaga, Kazuyoshi Ikuta, 他11名

    PLoS ONE   4 ( 1 )   2009.01

  • Detection of circulating Asian H5N1 viruses by a newly established monoclonal antibody

    Anariwa Du, Tomo Daidoji, Takaaki Koma, Madiha S. Ibrahim, Shota Nakamura, U. Chandimal de Silva, Mayo Ueda, Cheng-Song Yang, Teruo Yasunaga, Kazuyoshi Ikuta, Takaaki Nakaya

    Biochemical and Biophysical Research Communications   378   197 - 202   2009.01

  • H5N1 Avian Influenza Virus Induces Apoptotic Cell Death in Mammalian Airway Epithelial Cells

    Tomo Daidoji, Takaaki Koma, Anariwa Du, Cheng-Song Yang, Mayo Ueda, Kazuyoshi Ikuta, Takaaki Nakaya

    Journal of Virology   82 ( 22 )   11294 - 11307   2008.11

  • Maturation efficiency of viral glycoproteins in the ER impacts the production of influenza A virus

    Mayo Ueda, Masanobu Yamate, Anariwa Du, Tomo Daidoji, Yoshinobu Okuno, Kazuyoshi Ikuta, Takaaki Nakaya

    Virus Research   136   91 - 97   2008.09

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Books and Other Publications

  • 獣医公衆衛生学I

    　（ Role： Joint author）

    文永堂  2014 

Presentations

Outline of collaborative research (seeds)

  • ワクチン

  • 薬剤耐性菌

  • 人獣共通感染症

Grant-in-Aid for Scientific Research

  • 芽胞形成・毒素産生環境の制御に焦点をあてたウェルシュ菌食中毒予防に関する基礎研究

    Grant-in-Aid for Scientific Research(C)  2026

  • ゲノム情報から読み解く薬剤耐性感染症におけるキャリアとしての伴侶動物の重要性

    Grant-in-Aid for Scientific Research(C)  2026

  • 芽胞形成・毒素産生環境の制御に焦点をあてたウェルシュ菌食中毒予防に関する基礎研究

    Grant-in-Aid for Scientific Research(C)  2025

  • Immune response against monkeypox infection in post-vaccination immunization with smallpox vaccine

    Grant-in-Aid for Scientific Research(B)  2025

  • ゲノム情報から読み解く薬剤耐性感染症におけるキャリアとしての伴侶動物の重要性

    Grant-in-Aid for Scientific Research(C)  2025

  • 芽胞形成・毒素産生環境の制御に焦点をあてたウェルシュ菌食中毒予防に関する基礎研究

    Grant-in-Aid for Scientific Research(C)  2024

  • Immune response against monkeypox infection in post-vaccination immunization with smallpox vaccine

    Grant-in-Aid for Scientific Research(B)  2024

  • ゲノム情報から読み解く薬剤耐性感染症におけるキャリアとしての伴侶動物の重要性

    Grant-in-Aid for Scientific Research(C)  2024

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Charge of on-campus class subject

  • 獣医伝染病学B

    2024   Weekly class   Undergraduate

  • 獣医伝染病学A

    2024   Weekly class   Undergraduate

  • 人獣共通感染症学

    2024   Weekly class   Undergraduate

  • 獣医公衆衛生学

    2024   Weekly class   Undergraduate

  • 小動物基礎臨床実習

    2024   Intensive lecture   Undergraduate

  • 初年次ゼミナール

    2024   Weekly class   Graduate school

  • 感染症制御学特論

    2024   Intensive lecture   Graduate school

  • 獣医環境科学実習

    2024   Intensive lecture   Undergraduate

  • 獣医環境科学

    2024   Weekly class   Undergraduate

  • 獣医科学英語演習

    2024   Intensive lecture   Undergraduate

  • 獣医公衆衛生学実習

    2024   Weekly class   Undergraduate

  • 獣医伝染病学B

    2024   Weekly class   Undergraduate

  • 人獣共通感染症学

    2024   Weekly class   Undergraduate

  • 獣医公衆衛生学

    2023   Weekly class   Undergraduate

  • 小動物基礎臨床実習

    2023   Intensive lecture   Undergraduate

  • 獣医科学演習

    2023   Intensive lecture   Graduate school

  • 感染症制御学特論

    2023   Intensive lecture   Graduate school

  • 臨床基礎実習

    2023   Weekly class   Undergraduate

  • 食品衛生学実習

    2023   Intensive lecture   Undergraduate

  • 獣医環境科学実習

    2023   Intensive lecture   Undergraduate

  • 獣医環境科学

    2023   Weekly class   Undergraduate

  • 獣医公衆衛生学実習

    2023   Weekly class   Undergraduate

  • 獣医伝染病学B

    2023   Weekly class   Undergraduate

  • 人獣共通感染症学

    2023   Weekly class   Undergraduate

  • 獣医公衆衛生学

    2023   Weekly class   Undergraduate

  • 生物学実験

    2023   Intensive lecture   Graduate school

  • 獣医科学特別研究1

    2023   Intensive lecture   Graduate school

  • 獣医科学特別演習1

    2023   Intensive lecture   Graduate school

  • 獣医科学ミニレビュー

    2023   Intensive lecture   Graduate school

  • 獣医科学演習

    2023   Intensive lecture   Graduate school

  • 国際・国内インターンシップ

    2023   Intensive lecture   Graduate school

  • 獣医体験演習

    2023   Weekly class   Undergraduate

  • 生体環境制御学特別講義B

    2023   Intensive lecture   Graduate school

  • 小動物基礎臨床実習

    2022   Intensive lecture   Undergraduate

  • 獣医環境科学実習

    2022   Intensive lecture   Undergraduate

  • 獣医環境科学

    2022   Weekly class   Undergraduate

  • 獣医公衆衛生学実習

    2022   Weekly class   Undergraduate

  • 獣医伝染病学B

    2022   Weekly class   Undergraduate

  • 人獣共通感染症学

    2022   Weekly class   Undergraduate

  • 獣医科学特別研究1

    2022   Intensive lecture   Graduate school

  • 獣医科学特別演習1

    2022   Intensive lecture   Graduate school

  • 獣医公衆衛生学

    2022   Weekly class   Undergraduate

  • 食品衛生学実習

    2022   Intensive lecture   Undergraduate

  • 臨床基礎実習

    2022   Weekly class   Undergraduate

  • 獣医体験演習

    2022   Weekly class   Undergraduate

  • 生物学実験

    2022   Intensive lecture   Undergraduate

  • Veterinary Public Health

    2021    

  • Special Lecture: Bioenvironmental Sciences B

    2021    

  • Practice in Food Hygiene

    2021   Practical Training  

  • Practice in Veterinary Environmental Science

    2021   Practical Training  

  • Fundamental Experiments of Biology

    2021   Practical Training  

  • Practice in Veterinary Public Health

    2021   Practical Training  

  • Infectious Diseases of Animals B

    2021    

  • Veterinary Environmental Science

    2021    

  • Biology B

    2021    

  • Basic clinical practices

    2021   Practical Training  

  • Zoonotic Diseases

    2021    

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