Updated on 2023/07/05

写真a

 
SUEYOSHI Kenji
 
Organization
Graduate School of Engineering Division of Science and Engineering for Materials, Chemistry and Biology Associate Professor
School of Engineering Department of Applied Chemistry
Title
Associate Professor
Affiliation
Institute of Engineering
Affiliation campus
Nakamozu Campus

Position

  • Graduate School of Engineering Division of Science and Engineering for Materials, Chemistry and Biology 

    Associate Professor  2022.04 - Now

  • School of Engineering Department of Applied Chemistry 

    Associate Professor  2022.04 - Now

Degree

  • Doctor (Engineering) ( Kyoto University )

Research Areas

  • Nanotechnology/Materials / Analytical chemistry  / Electrophoretic Analysis

  • Life Science / Pharmaceutical analytical chemistry and physicochemistry

Research Interests

  • Microscale Electrophoresis

  • Microscale Bioassay

  • Selection and Analysis of Aptamers

Research subject summary

  • ミクロスケール電気泳動の高性能化・高感度化

  • ミクロスケール電気泳動技術を駆使した新規バイオアッセイ法の開発

  • ミクロスケール電気泳動に基づく核酸アプタマー選抜

Research Career

  • Development of Novel Bioassays based on Microscale Electrophoresis

    Microscale Electrophoresis, Microscale Bioassay, Online sample preconcentration, Enzyme-Activity Assay, Immunoassay  Individual

    2013.01 - Now 

  • Aptamer Selection based on Microscale Electrophoresis

    capillary electrophoresis, microchip electrophoresis, aptamer selection  Individual

    2016.04 - Now 

  • ミクロスケール電気泳動技術を駆使した細胞外微粒子解析

    細胞外小胞,エクソソーム,粒子径分級 

    2019 - Now 

Professional Memberships

  • 日本分析化学会

    2003.04 - Now

  • 日本化学会

    2005.04 - Now   Domestic

  • 化学とマイクロ・ナノシステム学会

    2003.04 - Now   Domestic

  • クロマトグラフィー科学会

    2005.04 - Now   Domestic

Committee Memberships (off-campus)

  • 理事   クロマトグラフィー科学会  

    2022.04 - Now 

  • 評議員   クロマトグラフィー科学会  

    2013.04 - 2022.03 

Awards

  • CREST 細胞外微粒子 Young Innovator Award

    末吉健志

    2023.02   国立研究開発法人 科学技術振興機構 CREST 細胞外微粒子領域   アプタマー修飾ハイドロゲルビーズを用いた選択的なEV迅速捕捉・濃縮・回収・機能解析手法の開発

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    CREST細胞外微粒子領域における新規研究内容として、アプタマー修飾ハイドロゲルビーズを用いた電気泳動技術に基づく細胞外微粒子の選択的・迅速捕捉・濃縮・回収・機能解析手法を新規提案した。

  • 若手優秀賞

    2015.06   化学とマイクロ・ナノシステム学会  

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    Country:Japan

  • 奨励賞

    2014.09   日本分析化学会  

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    Country:Japan

  • 奨励賞

    2011.10   クロマトグラフィー科学会  

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    Country:Japan

Job Career (off-campus)

  • JST CREST研究員

    2023.04 - Now

  • JSTさきがけ研究員

    2019.10 - 2023.03

  • 京都大学大学院工学研究科 助教

    2009.04 - 2012.12

  • 日本学術振興会特別研究員(PD)

    2008.04 - 2009.03

  • 日本学術振興会特別研究員(DC2)

    2007.04 - 2008.03

Education

  • Kyoto University   Graduate School, Division of Engineering   Material Chemistry   The second semester of doctoral program   Graduated/Completed

    2005.04 - 2008.03

  • Kyoto University   Graduate School, Division of Engineering   Material Chemistry   The first semester of doctoral program   Graduated/Completed

    2003.04 - 2005.03

  • Kyoto University   Faculty of Engineering   Industrial Chemistry   Bachelor's Course   Graduated/Completed

    1998.04 - 2003.03

Papers

  • Investigation of Probe DNA Immobilization Method for Label-free DNA Biosensing Using Polymer-based Photonic Crystal

    Ryosuke Nishitsuji, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Sensors and Materials   35 ( 3 )   1035 - 1035   2023.03( ISSN:0914-4935

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.18494/sam4329

  • Single Cycle Selection of CD63-targeting Aptamers Using a Microscale Electrophoretic Filtration Device

    TOBITA Arisa, TAKAO Junku, ENDO Tatsuro, HISAMOTO Hideaki, SUEYOSHI Kenji

    BUNSEKI KAGAKU   72 ( 3 )   111 - 116   2023.03( ISSN:05251931

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    <p>Aptamers, which show properties of specific binding to their target molecules, are expected to become an alternative to antibodies for use as molecular recognition probes instead of antibodies. However, long and laborious selection procedures have hindered their application. To overcome the drawbacks, rapid and simple selection methods using a microscale electrophoretic filtration device have been developed. In the first study, the aptamers for immunoglobulin E were successfully selected by three cycle selection based on the developed method. To realize more rapid and simple selection, aptamer selection by single cycle procedure was demonstrated. It was confirmed that CD63 as a model target was successfully trapped by the capillary device partially plugged with a polyacrylamide hydrogel. A DNA library containing various sequences were then electrokinetically introduced into the device after trapping CD63, a large volume of DNAs bound with the trapped CD63. After washing and elution schemes, DNAs located in the eluents were enhanced by PCR, and then their sequences were analyzed by next generation sequencer. As a result, one of the obtained aptamer candidates shows similar motifs to the previously reported aptamer. Isothermal titration calorimetry verified the binding ability and specificity of the obtained candidate to CD63. Consequently, it was clarified that the developed method and device is applicable to the rapid and simple selection of aptamers using the single cycle procedure.</p>

    DOI: 10.2116/bunsekikagaku.72.111

  • Combining TiO<inf>2</inf> photonic crystals and reagents enhances the sensitivity of endotoxin detection

    Nishitsuji R.

    Chemical Papers   77 ( 1 )   611 - 615   2023.01( ISSN:03666352

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  • Nanoemulsion-based silver ion-selective optode based on colorimetrically silver ion-responsive ionic liquid-based dye

    Oka S.

    Analytical Sciences   2023( ISSN:09106340

  • Aptamer Selection Based on Microscale Electrophoretic Filtration Using a Hydrogel-Plugged Capillary Device.

    Junku Takao, Reina Nagai, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi

    Molecules (Basel, Switzerland)   27 ( 18 )   2022.09

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    This study reports a novel aptamer selection method based on microscale electrophoretic filtration. Aptamers are versatile materials that recognize specific targets and are attractive for their applications in biosensors, diagnosis, and therapy. However, their practical applications remain scarce due to issues with conventional selection methods, such as complicated operations, low-efficiency separation, and expensive apparatus. To overcome these drawbacks, a selection method based on microscale electrophoretic filtration using a capillary partially filled with hydrogel was developed. The electrophoretic filtration of model target proteins (immunoglobulin E (IgE)) using hydrogel, the electrokinetic injection of DNAs to interact with the trapped proteins, the elimination of DNAs with weak interactions, and the selective acquisition of aptamer candidates with strong interactions were successfully demonstrated, revealing the validity of the proposed concept. Two aptamer candidates for IgE were obtained after three selection cycles, and their affinity for the target was confirmed to be less than 1 nM based on their dissociation constant (KD) values. Therefore, the proposed method allows for the selection of aptamers with simple operations, highly effective separation based on electrophoresis and filtration, and a relatively cheap apparatus with disposable devices.

    DOI: 10.3390/molecules27185818

    PubMed

  • Fabrication of Gold Nanostructures on Quartz Crystal Microbalance Surface Using Nanoimprint Lithography for Sensing Applications.

    Ryosuke Nishitsuji, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Micromachines   13 ( 9 )   2022.08( ISSN:2072-666X

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    A quartz crystal microbalance (QCM) is a sensor that uses the piezoelectric properties of quartz crystals sandwiched between conductive electrodes. Localized surface plasmon resonance (LSPR) is an analytical technique that uses the collective vibration of free electrons on metal surfaces. These measurements are known as analysis techniques that use metal surfaces and have been applied as biosensors because they allow for the label-free monitoring of biomolecular binding reactions. These measurements can be used in combination to analyze the reactions that occur on metal surfaces because different types of information can be obtained from them. However, as different devices are used for these measurements, the results often contain device-to-device errors and are not accurately evaluated. In this study, we directly fabricated gold nanostructures on the surface of a QCM to create a device that can simultaneously measure the mass and refractive index information of the analyte. In addition, the device could be easily fabricated because nanoimprint lithography was used to fabricate gold nanostructures. As a proof of concept, the nanoparticle adsorption on gold nanostructures was evaluated, and it was observed that mass and refractive index information were successfully obtained without device-to-device errors.

    DOI: 10.3390/mi13091430

    PubMed

  • Gold Nanocone Array with Extensive Electromagnetic Fields for Highly Reproducible Surface-Enhanced Raman Scattering Measurements.

    Satoko Fujiwara, Daiki Kawasaki, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Micromachines   13 ( 8 )   2022.07( ISSN:2072-666X

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Surface-enhanced Raman scattering (SERS) is a technique used to distinguish the constitution of disease-related biomarkers in liquid biopsies, such as exosomes and circulating tumor cells, without any recognition elements. Previous studies using metal nanoparticle aggregates and angular nanostructures have achieved the detection of various biomarkers owing to strong hot spots and electromagnetic (EM) fields by localized surface plasmon resonance (LSPR). Although these SERS platforms enable significant enhancement of Raman signals, they still have some problems with the fabrication reproducibility of platforms in obtaining reproducible SERS signals. Therefore, highly reproducible fabrication of SERS platforms is required. Here, we propose the application of a polymer-based gold (Au) nanocone array (Au NCA), which extensively generates an enhanced EM field near the Au NCA surface by LSPR. This approach was experimentally demonstrated using a 785 nm laser, typically used for SERS measurements, and showed excellent substrate-to-substrate reproducibility (relative standard deviation (RSD) &lt; 6%) using an extremely simple fabrication procedure and very low laser energy. These results proved that a Au NCA can be used as a highly reproducible SERS measurement to distinguish the constitution of biomarkers.

    DOI: 10.3390/mi13081182

    PubMed

  • Quantum dots / TiO2 hybrid photonic crystal: Fabrication and application for highly sensitive and visible region-responsive biosensor

    Shotaro Ueda, Daiki Kawasaki, Ryosuke Nishitsuji, Hirotaka Yamada, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    MICROELECTRONIC ENGINEERING   263   2022.07( ISSN:0167-9317

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    Publishing type:Research paper (scientific journal)  

    In this work, by combining a photonic crystal (PhC) and highly luminescent material, we developed an extremely sensitive PhC sensor that allows detection by single wavelength fluorescence intensity change in the visible wavelength region. We fabricated a titanium dioxide (TiO2) PhC containing quantum dots (QDs) inside the PhC structure and confirmed that the fluorescence intensity of the QDs was enhanced. Fluorescence enhancement occurs because of the total reflection and light confinement of the QD fluorescence inside the TiO2 PhC structure. The response to changes in the surrounding refractive index and protein adsorption was confirmed. Furthermore, for biosensing applications, troponin T (TnT), an acute myocardial marker, was determined using an antigen -antibody reaction. It was found that a TnT level of 100 fg/mL, which is considerably less than the cutoff value of 100 pg/mL, was successfully detected. The lower limit of detection (LOD) was found to be 58.2 fg/mL, which is 1/40 of that of the conventional enzyme-linked immunosorbent assay (ELISA) method (LOD: 2 pg/mL). It was demonstrated that the present TiO2 PhC-containing QDs can be used as a sensor device for the highly sensitive detection and determination of disease biomarkers.

    DOI: 10.1016/j.mee.2022.111842

  • Development of transient trapping micellar electrokinetic chromatography coupled with mass spectrometry for steroids analysis

    Yanawut Manmana, Chenchen Liu, Hiroshi Koino, Kenji Sueyoshi, Fumihiko Kitagawa, Takuya Kubo, Koji Otsuka

    Chirality   34 ( 10 )   1328 - 1337   2022.06( ISSN:0899-0042

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/chir.23489

    Other URL: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/chir.23489

  • 【高感度解析に寄与する分離分析技術】デジタル分子ふるい電気泳動用キャピラリーデバイスの開発

    末吉 健志, 松田 景太, 遠藤 達郎, 久本 秀明

    分析化学   71 ( 6 )   325 - 331   2022.06( ISSN:0525-1931

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    デジタル電気泳動法は、各種機能性ヒドロゲルに対する試料分子の通過・非通過を利用した分離・濃縮法として近年注目されているが、ヒドロゲル充填デバイス作製の煩雑さが大きな課題であった。そこで、本研究では、簡易デバイスを用いたデジタル電気泳動技術の開発と、機能性ヒドロゲルによるタンパク質の分離・濃縮評価のため、異種ポリアクリルアミドゲル連続充填キャピラリーデバイスを作製し、デジタル分子ふるい電気泳動の基礎評価を行った。その結果、プレポリマー溶液中アクリルアミド総濃度(%T)と通過・非通過タンパク質の分子量との相関が明らかとなった。また、濃縮効率評価の結果、最大で約180倍の高感度化が確認された。さらに、5、15、25%Tポリアクリルアミドゲル連続充填デバイスを用いたデジタル分子ふるい電気泳動によって、分子量が異なるタンパク質の分離と濃縮が同時に達成された。以上の結果から、作製したデバイスを用いた簡便・高分離能・高感度なデジタル電気泳動が実証された。(著者抄録)

  • Wavelength-tunable dual-band edge plasmon mode based on gold edge-hole plasmonic nanostructure

    Hirotaka Yamada, Daiki Kawasaki, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    RESULTS IN PHYSICS   37   2022.06( ISSN:2211-3797

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    Publishing type:Research paper (scientific journal)  

    Metal nanostructures have attracted attention because of their unique optical characteristics derived from localized surface plasmon resonance. In this study, gold nanoholes with edge structures were designed and characterized to possess two plasmon bands at different wavelengths. These bands were assigned to the plasmon modes that generated a concentrated electric field at the tip of the individual edge structures. Furthermore, wavelengths of these bands were independently tuned from the visible to near-infrared wavelengths with shape and size of the gold nanoholes and edge structures as variables. To demonstrate wavelength tuning, a gold nanostructure with edge plasmon bands at wavelengths of approximately 785 and 1064 nm (typical laser wavelengths for surface-enhanced Raman scattering and optical tweezers, respectively) was successfully obtained via optical simulation. The results indicate that the structure designed in this study provides a guideline for the design of optical devices with efficient multiple optical functions.

    DOI: 10.1016/j.rinp.2022.105541

  • Evaluation of the interactions between oligonucleotides and small molecules by partial filling-nonequilibrium affinity capillary electrophoresis.

    Eriko Mitsuno, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry   38 ( 6 )   851 - 859   2022.06

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Recently, high-throughput analysis with minimal reagent consumption has been desired to assess interactions between drug candidates and disease-related oligonucleotides. To realize an ideal assay for drug screening, a rapid assay based on affinity capillary electrophoresis was generated to reduce the consumption of samples/reagents by a partial-filling technique under nonequilibrium conditions. In the proposed method, the first sample, oligonucleotide as a ligand, and second sample zones were injected into a capillary with spacers of background solution between the samples and oligonucleotide zones. After applying voltages, only the second sample zone passed through the partially filled oligonucleotide zone, resulting in variations in the peak parameters owing to this interaction. The electropherograms obtained were analyzed using equilibrium, reaction kinetics, and moment theories. In the interaction analyses between small molecules and DNA aptamers, only the small molecules binding to the aptamer showed significant changes in their peak heights and shapes. The estimated kinetic parameters were in good agreement with the reported values, indicating the applicability of the proposed method for drug screening. When interactions between drug candidates and disease-related RNAs were analyzed, one of the candidates showed remarkable variation in the peak parameters upon the addition of potassium ions. Consequently, the proposed method could be one of the ideal assays for drug screening.

    DOI: 10.1007/s44211-022-00101-x

    PubMed

  • Au nanorods-TiO2 photonic crystal plasmonic-photonic hybrid sensor for label-free detection and identification of DNA molecules with single nucleotide polymorphisms

    Daiki Kawasaki, Hirotaka Yamada, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    SENSORS AND ACTUATORS B-CHEMICAL   361   2022.06

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    Publishing type:Research paper (scientific journal)  

    Highly sensitive optical sensors for label-free detection of DNA must have high usability and wide applicability for achieving advances in the medical and biological science fields. However, current sensors do not simultaneously satisfy these criteria. Here, we developed a simple, cost-effective, and sensitive plasmonic-photonic hybrid sensor using gold nanorods as nanoantennae and a photonic crystal slab (PCS) as a microcavity. In this report, we performed simple microscopic detection and identification of DNA molecules with Alzheimer's disease-associated single nucleotide polymorphisms, which is an important indicator for early diagnosis. The PCS was fabricated using nanoimprint lithography; the gold nanorods were controllably coupled with the PCS. The device sensitivity was experimentally assessed by controlling the plasmonic-photonic coupling resonance, which was enabled by tuning the PCS-coupled AuNR amounts. The sensitivity was also investigated by theoretical modeling of the hybrid sensor, based on the temporal coupled mode theory (TCMT). The TCMT-based theoretical model well explained the experimental results. In a demonstration of ultrasensitive detection of DNA, the identification of single nucleotide mismatched DNA molecules (similar to 1 pM) and detection of several DNA molecules (similar to 1000), with a limit of detection of 5.9 aM, were successfully achieved. This sensor may thus have wide applications for DNA analysis.

    DOI: 10.1016/j.snb.2022.131747

  • Chloride ion-selective dye liquid nanoemulsion: improved sensor performance due to intermolecular interactions between dye and ionophore.

    Kaho Maki, Ryoutarou Oishi, Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    The Analyst   147 ( 8 )   1529 - 1533   2022.04

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Ionophore-based dye liquid nanoemulsion sensors exhibiting rapid response, high selectivity, and high sensitivity to chloride were developed. Since nanoemulsions contain extremely high concentrations of dyes and have large surface areas, rapid and highly sensitive measurements were possible. 1H-NMR measurements revealed intermolecular interactions between the dye and the ionophore, which contributed to the suppression of the background signal.

    DOI: 10.1039/d2an00115b

    PubMed

  • Imprinted Photonic Crystal-Film-Based Smartphone-Compatible Label-Free Optical Sensor for SARS-CoV-2 Testing.

    Daiki Kawasaki, Hirotaka Yamada, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Biosensors   12 ( 4 )   2022.03

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    The coronavirus disease (COVID-19) caused by SARS-CoV-2 has caused a global pandemic. To manage and control the spread of the infection, it is crucial to develop and implement technologies for the early identification of infected individuals and rapid informatization in communities. For the realization of such a technology, a widely available and highly usable sensor for sensitive and specific assay of the virus plays a fundamental role. In this study, we developed an optical sensor based on an imprinted photonic crystal film (IPCF) for quick, simple, and cost-effective detection of SARS-CoV-2 spike protein in artificial saliva. Our IPCF sensor enabled label-free and highly sensitive detection with a smartphone-equipped optical setup. The IPCF surface was functionalized with an anti-SARS-CoV-2 spike protein antibody for immunoassay. We evaluated the specificity and sensitivity of the IPCF sensor for quantitative detection of the spike protein in artificial saliva using simple reflectometry with a spectrometer-equipped optical setup. Specific and quantitative detection of the spike protein was successfully achieved, with a low detection limit of 429 fg/mL. In the demonstration of reflectometric detection with a smartphone-equipped setup, the sensitivity was comparable with that with a spectrometer-equipped setup. The test result is returned immediately and can be saved to cloud storage. In addition, it costs less than USD 1 for one IPCF to be used for diagnosis. Thus, the developed IPCF has the potential to realize a widely available and highly usable sensor.

    DOI: 10.3390/bios12040200

    PubMed

  • Fabrication of Metal-Insulator-Metal Nanostructures Composed of Au-MgF2-Au and Its Potential in Responding to Two Different Factors in Sample Solutions Using Individual Plasmon Modes.

    Hirotaka Yamada, Daiki Kawasaki, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Micromachines   13 ( 2 )   2022.02

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    In this paper, metal-insulator-metal (MIM) nanostructures, which were designed to exhibit two absorption peaks within 500-1100 nm wavelength range, were fabricated using magnesium difluoride (MgF2) as the insulator layer. Since the MIM nanostructures have two plasmon modes corresponding to the absorption peaks, they independently responded to the changes in two phases: the surrounding medium and the inside insulator layer, the structure is expected to obtain multiple information from sample solution: refractive index (RI) and molecular interaction between solution components and the insulator layer. The fabricated MIM nanostructure had a diameter of 139.6 ± 2.8 nm and a slope of 70°, and exhibited absorption peaks derived from individual plasmon modes at the 719 and 907 nm wavelengths. The evaluation of the response to surrounding solution component of the MIM nanostructures revealed a linear response of one plasmon mode toward the RI of the surrounding medium and a large blue shift of the other plasmon mode under conditions where glycerol was present at high concentration. From optical simulation and the evaluation of the MgF2 fabricated by deposition, the blue shift was expected to be due to the swelling of MgF2 interacting with the hydroxyl groups abundantly included in the glycerol molecules. The results indicated the individual responses of two plasmon modes in MIM nanostructures toward medium components, and brought the prospect for the simultaneous measurement of multiple elements using two or more plasmon modes.

    DOI: 10.3390/mi13020257

    PubMed

  • Development of Capillary Devices for Digital Molecular Sieving Electrophoresis

    Kenji Sueyoshi, Keita Matsuda, Tatsuro Endo, Hideaki Hisamoto

    BUNSEKI KAGAKU   71 ( 6 )   325 - 331   2022( ISSN:0525-1931

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    Publishing type:Research paper (scientific journal)  

    Recently, digital electrophoresis has attracted attention as a separation and preconcentration method based on the passage or non-passage of sample molecules through various functional hydrogels. However, the complex fabrication of the device filled with various hydrogels has been a major issue. In this study, an easy-to-fabricate capillary device for digital electrophoresis is proposed and developed by the successive introduction of prepolymer solutions. Digital molecular sieving electrophoresis using polyacrylamide hydrogels with differcni densities was employed for a fundamental characterization of the developed device. As a result, the correlation between the total concentration of acrylamide species in the prepolymer solution (%T) and the molecular weights of the passing or non-passing proteins was clarified. The evaluation of the concentration efficiency showed that the sensitivity increased up to about 180 times. In addition, digital molecular sieve electrophoresis using the device successively plugged with 5, 15, and 25 %T polyacrylamide hydrogels achieved simultaneous separation and preconcentration of proteins with different molecular weights. These results demonstrate high-resolution with high-sensitivity digital electrophoresis using the simply fabricated device.

  • Modulating Optical Characteristics of Nanoimprinted Plasmonic Device by Re-Shaping Process of Polymer Mold.

    Hirotaka Yamada, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Micromachines   12 ( 11 )   2021.10

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Metal nanostructures exhibit specific optical characteristics owing to their localized surface plasmon resonance (LSPR) and have been studied for applications in various optical devices. The LSPR property strongly depends on the size and shape of metal nanostructures; thus, plasmonic devices must be designed and fabricated according to their uses. Nanoimprint lithography (NIL) is an effective process for repeatedly fabricating metal nanostructures with controlled sizes and shapes and require optical properties. NIL is a powerful method for mass-producible, low-cost, and large-area fabrication. However, the process lacks flexibility in adjusting the size and shape according to the desirable optical characteristics because the size and shape of metal nanostructures are determined by a single corresponding mold. Here, we conducted a re-shaping process through the air-plasma etching of a polymer's secondary mold (two-dimensional nanopillar array made of cyclo-olefin polymer (COP)) to modulate the sizes and shapes of nanopillars; then, we controlled the spectral characteristics of the imprinted plasmonic devices. The relationship between the structural change of the mold, which was based on etching time, and the optical characteristics of the corresponding plasmonic device was evaluated through experiments and simulations. According to evaluation results, the diameter of the nanopillar was controlled from 248 to 139 nm due to the etching time and formation of a pit structure. Consequently, the spectral properties changed, and responsivity to the surrounding dielectric environment was improved. Therefore, plasmonic devices based on the re-shaped COP mold exhibited a high responsivity to a refractive index of 906 nm/RIU at a wavelength of 625 nm.

    DOI: 10.3390/mi12111323

    PubMed

  • インクジェットプリンティングによる、マイクロチャネルアレイデバイス固定化酵素と蛍光基質を用いた1ステップトリプシンインヒビターアッセイ(Single-step Trypsin Inhibitor Assay on a Microchannel Array Device Immobilizing Enzymes and Fluorescent Substrates by Inkjet Printing)

    Kawai Yuko, Ideggami Kotaro, Sueyoshi Kenji, Endo Tatsuro, Hisamoto Hideaki

    Analytical Sciences   37 ( 10 )   1473 - 1476   2021.10( ISSN:0910-6340

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    本報ではインクジェットプリンティングを用いて、反応試薬を固定化したバイオアッセイマイクロデバイスによる、1ステップトリプシンインヒビターアッセイデバイスについて報告した。なお、本デバイスは、ポリジルメチルシロキサン(PDMS)製マイクロチャネルアレイで構成されており、インクジェットプリンターにより、マイクロチャネル底面に試薬をナノリットルサイズの液滴として、簡便且つ正確に位置選択的に固定化することが可能である。また、本デバイスにより、トリプシンと蛍光基質が互いに混合することなく、同じマイクロチャネル内の異なる位置にそれぞれ固定化された。さらに、トリプシン固定化のため、スポッティング剤に0.8mM HCl溶液に加えると、トリプシンの自己消化により、蛍光強度低下が抑制されることも明らかにされ、本デバイスを用いた、1ステップトリプシンインヒビタースクリーニングでは、ロイペプチンを含む3種類のトリプシンインヒビターにおいて、トリプシン阻害作用も確認された。本デバイスは10日間以上も安定して保存が可能で、位置ずれによるばらつきの問題も改善されたことからも、大量生産が期待され、本デバイスが創薬分野で、種々の酵素阻害剤アッセイに有用性が高いことが示唆された。

  • Enzyme-responsive fluorescent nanoemulsion based on lipophilic dye liquid.

    Ryoutarou Oishi, Kaho Maki, Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    The Analyst   146 ( 13 )   4121 - 4124   2021.06

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    An enzyme-responsive fluorescent nanoemulsion (NE) based on lipophilic dye liquid (LDL) was developed for alkaline phosphatase (ALP). The response mechanism of the NE involved enzymatic reactions and simultaneous extraction of anions. The LDL-based NE exhibited 3.8 times higher sensitivity than plasticizer-based conventional NE. Detection limit and response range were 2.7 (U L-1) and 5-50 (U L-1), respectively. The response time was reduced to less than half that of the LDL-based membrane.

    DOI: 10.1039/d1an00447f

    PubMed

  • Fractionation of Single-stranded DNAs with/without Stable Preorganized Structures Using Capillary Sieving Electrophoresis for Aptamer Selection.

    Masahide Wada, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry   37 ( 6 )   799 - 802   2021.06

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    Aptamers, single-stranded DNAs/RNAs with a strong and specific interaction towards a target molecule, have wide applications in the fields of medicine and biosensors. In conventional aptamer selection methods, it is difficult to obtain "preorganized" and/or "induced-fit" type of aptamers selectively. In this study, separation and fractionation of single-stranded DNAs with/without stable preorganized structures were carried out using capillary sieving electrophoresis. The fractionated DNAs showed different mobilities and thermodynamic stabilities of their secondary structures; this outcome is deemed to be necessary for the synthesis of novel aptasensors with a desirable sensing mechanism.

    DOI: 10.2116/analsci.21C003

    PubMed

  • アプタマー選択のためのキャピラリー篩電気泳動法を用いた、安定的な事前に組織化した構造の有無による一本鎖DNAの分画(Fractionation of Single-stranded DNAs with/without Stable Preorganized Structures Using Capillary Sieving Electrophoresis for Aptamer Selection)

    Wada Masahide, Endo Tatsuro, Hisamoto Hideaki, Sueyoshi Kenji

    Analytical Sciences   37 ( 6 )   799 - 802   2021.06( ISSN:0910-6340

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    キャピラリー篩電気泳動法を用いて、安定的な事前に組織化された構造を持つ一本鎖DNAと持たない一本鎖DNAの分離・分画した。その結果、分画したDNAはその二次構造とは異なった移動度と熱力学的安定性を示した。これらの結果は、望ましい感知機序を持つ新規アプタセンサーの合成に必要と考えられた。

  • Fractionation of Single-stranded DNAs with/without Stable Preorganized Structures Using Capillary Sieving Electrophoresis for Aptamer Selection

    Masahide Wada, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi

    ANALYTICAL SCIENCES   37 ( 6 )   799 - 802   2021.06( ISSN:0910-6340

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    Publishing type:Research paper (scientific journal)  

    Aptamers, single-stranded DNAs/RNAs with a strong and specific interaction towards a target molecule, have wide applications in the fields of medicine and biosensors. In conventional aptamer selection methods, it is difficult to obtain "preorganized" and/or "induced-fit" type of aptamers selectively. In this study, separation and fractionation of single-stranded DNAs with/without stable preorganized structures were carried out using capillary sieving electrophoresis. The fractionated DNAs showed different mobilities and thermodynamic stabilities of their secondary structures; this outcome is deemed to be necessary for the synthesis of novel aptasensors with a desirable sensing mechanism.

    DOI: 10.2116/analsci.21C003

  • Single-Step Trypsin Inhibitor Assay on a Microchannel Array Device Immobilizing Enzymes and Fluorescent Substrates by Inkjet Printing.

    Yuko Kawai, Kotaro Idegami, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry   37 ( 10 )   1473 - 1476   2021.04

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:Domestic journal  

    In this paper, we report a single-step trypsin inhibitor assay on a microchannel array device immobilizing enzymes and substrates by inkjet printing. The microdevice is composed of a poly(dimethylsiloxane) (PDMS) microchannel array that immobilizes trypsin and fluorescent substrates as reactive reagents at the two bottom corners of a microchannel. Inkjet printers allow simple, accurate, and position-selective immobilization of reagents as nanoliter spots. Therefore, plural reactive reagents, such as enzymes and substrates, can be separately immobilized at different positions in the same microchannel without mixing, and thus allowing for single-step operation by simply introducing a sample solution through capillary action. Furthermore, reproducible fabrication and mass production of the device could be expected. In this study, the efficiency of an acidic solution as a spotting agent for protease immobilization to prevent decrease in the fluorescence intensity was confirmed. Additionally, single-step trypsin inhibitor screening was performed using three inhibitors. Finally, we investigated the storage stability of the device and confirmed that it remained stable for at least 10 days.

    DOI: 10.2116/analsci.21N011

    PubMed

  • Direct measurement of initial rate of enzyme reaction by electrokinetic filtration using a hydrogel-plugged capillary device.

    Junku Takao, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry   37 ( 10 )   1439 - 1446   2021.04

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:Domestic journal  

    A novel electrokinetic filtration device using a plugged hydrogel was developed to directly measure the initial rate of enzyme reactions. In the proposed method, the enzyme reaction proceeded only for a short time when the substrate was passed through a thin layer of enzyme trapped by the hydrogel without any lag times for mixing and detection. In experimental conditions, alkaline phosphatase (enzyme) was filtrated at a cathodic-side interface of the plugged hydrogel by molecular sieving effect, providing the thin enzyme zone whose thickness was approximately 100 μm. When 4-methylumberiferyl phosphate (substrate) was electrokinetically introduced into the device after trapping the enzyme, 4-methylumberiferone (product) was generated by the enzyme reaction for only 1.26 s as the substrate passed through the trapped enzyme zone. As a result, the initial rate of the enzyme reaction could be directly calculated to 31.0 μM/s by simply dividing the concentration of the product by the tunable reaction time. Compared to the initial rate obtained by mixing the enzyme and substrate solutions, the value of the maximum velocity of the enzyme reaction was 30-fold larger than that in the mixing method due to the preconcentration of the enzyme by trapping. The Michaelis-Menten constant in the proposed method was 2.7-fold larger than that in the mixing method, suggesting the variation of changes in the equilibrium of complex formation under the experimental conditions.

    DOI: 10.2116/analsci.21P067

    PubMed

  • Lipophilic Fluorescent Dye Liquids: Förster Resonance Energy Transfer-Based Fluorescence Amplification for Ion Selective Optical Sensors Based on a Solvent Polymeric Membrane.

    Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Analytical chemistry   93 ( 9 )   4143 - 4148   2021.03

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Optical sensors based on solvent polymeric membranes have the potential to measure analytes present in an aqueous solution through the development of a tailored method for a specific target. However, limits in the concentrations of the component dyes have prevented improvements in sensitivity. We propose a Förster resonance energy transfer (FRET)-based fluorescence amplification system for ion-selective optical sensors using a highly fluorescent liquid material composed of a lipophilic phosphonium cation and a pyrene modifying sulfonate anion ([P66614][HP-SO3]), as both the plasticizer and donor, in addition to a combination of the lipophilic phosphonium cation and the fluorescein dodecyl ester anion ([P66614][12-FL]) as the fluorescent sensing dye acceptor. For ion extraction-based sensing, the donor and acceptor were retained in the plasticized PVC membrane with negligible leaching upon exposure to acidic and basic aqueous solutions. Systematic investigation of the donor and acceptor ratios clarified the effect of the amplification factor and the sensitivity of the sensor. At an acceptor doping level of 0.5 mol % (vs donor), an approximately 22-fold higher sensitivity was obtained compared to that of a conventional PVC membrane optical sensor. During ion measurement based on the coextraction of protons and anions, selectivity following the Hofmeister order was observed, which was controlled by the addition of ionophores. The proposed FRET system based on a lipophilic fluorescent liquid material has the potential to significantly improve the sensitivities of optical sensors using solvent polymeric membranes with high selectivities for various target analytes.

    DOI: 10.1021/acs.analchem.0c05007

    PubMed

  • 【化学センシングの方法論・デバイス開発の新展開】インクジェットプリンティングによる試薬固定化法を利用する1ステップバイオアッセイマイクロデバイスの開発

    河相 優子, 白井 亮洋, 角田 正也, 井手上 公太郎, 末吉 健志, 遠藤 達郎, 久本 秀明

    分析化学   70 ( 3 )   125 - 131   2021.03( ISSN:0525-1931

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    本研究では、インクジェットプリンティングを用いて一つのポリジメチルシロキサン(Poly(dimethylsiloxane)、PDMS)製マイクロ流路内に2種類の反応試薬を固定化した1ステップ均一系競合型バイオアッセイマイクロデバイスを開発した。インクジェットプリンターは試薬をナノリットルサイズの液滴として正確な量を位置選択的に吐出するため、互いに反応する2種類の試薬を混ざることなく微少空間内に独立して固定化できる。そのため、先行研究でデバイスを作製する際に課題となった、相互に反応する試薬を固定化した二つのマイクロ流路を精密に組み合わせる操作が不要となる。本研究ではその一例として、ビオチン固定化酸化グラフェン(BG)と蛍光標識ストレプトアビジン(F-SA)を1本のマイクロ流路内に固定してビオチンアッセイマイクロデバイスを試作し、基礎検討として試薬の固定化位置や試薬及び添加剤種の評価を行った。その結果、液滴を流路壁面寄りに吐出すると再現性よく流路底面の両サイドに試薬固定できること、添加剤としてトレハロースが適すること及び、試料導入操作のみでの1ステップ検出で、検出下限は0.68ng mL-1となり、先行研究と比較して約1/600倍改善することを明らかにした。(著者抄録)

  • Lipophilic Fluorescent Dye Liquids: Förster Resonance Energy Transfer-Based Fluorescence Amplification for Ion Selective Optical Sensors Based on a Solvent Polymeric Membrane Reviewed

    Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Analytical Chemistry 雑誌   93 ( 9 )   2021.02

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    Kind of work:Joint Work  

  • Highly sensitive optical ion sensor with ionic liquid-based colorimetric membrane/photonic crystal hybrid structure.

    Daiki Kawasaki, Ryoutarou Oishi, Nao Kobayashi, Tatsumi Mizuta, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Scientific reports   10 ( 1 )   16739 - 16739   2020.10

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    An ionic liquid-based thin (~ 1 µm) colorimetric membrane (CM) is a key nano-tool for optical ion sensing, and a two-dimensional photonic crystal slab (PCS) is an important nano-platform for ultimate light control. For highly sensitive optical ion sensing, this report proposes a hybrid of these two optical nano-elements, namely, a CM/PCS hybrid. This structure was successfully fabricated by a simple and rapid process using nanoimprinting and spin-coating, which enabled control of the CM thickness. Optical characterization of the hybrid structure was conducted by optical measurement and simulation of the reflection spectrum, indicating that the light confined in the holes of the PCS was drastically absorbed by the CM when the spectrum overlapped with the absorption spectrum of the CM. This optical property obtained by the hybridization of CM and PCS enabled drastic improvement in the absorption sensitivity in Ca ion sensing, by ca. 78 times compared to that without PCS. Experimental and simulated investigation of the relation between the CM thickness and absorption sensitivity enhancement suggested that the controlled light in the PCS enhanced the absorption cross-section of the dye molecules within the CM based on the enhanced local density of states. This highly sensitive optical ion sensor is expected to be applied for micro-scale bio-analysis like cell-dynamics based on reflectometric Ca ion detection.

    DOI: 10.1038/s41598-020-73858-8

    PubMed

  • A lipophilic ionic liquid-based dye for anion optodes: importance of dye lipophilicity and application to heparin measurement.

    Tatsumi Mizuta, Shu Takai, Toshiki Nishihata, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    The Analyst   145 ( 16 )   5430 - 5437   2020.08

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Herein, a fully lipophilic ionic liquid (IL) comprising a lipophilic fluorescein anion and a trihexyltetradecylphosphonium cation was synthesized and used as the plasticizer for a plasticized poly(vinyl chloride) (PVC) membrane optode. Systematic investigation of the alkyl chain length of the fluorescein anion proved the significance of lipophilicity for obtaining the reversible absorbance measurements. A PVC membrane fabricated with the synthesized lipophilic IL was observed to comprise an unusually high dye concentration (915 mmol kg-1) and exhibited good sensitivity as well as response time in its sensor performance. The sensitivity of the presented PVC membrane was 26-fold higher than that of a conventional optode membrane with the same membrane thickness and the same lipophilic dye of typical dye content (1 wt%). The response time was observed to be >120-fold faster by using a significantly thinner PVC membrane (approx. 140 nm). Heparin is known to be a polyanionic anticoagulant, and the presented PVC membrane exhibited an extremely fast response (20-150 seconds) to the heparin in diluted serum within the required concentration region. Thus, the lipophilic IL-based dye could significantly improve the sensor performance in conventional optodes, especially for an analyte showing slow diffusion, such as macromolecular heparin.

    DOI: 10.1039/d0an00335b

    PubMed

  • Highly sensitive optical ion sensor with ionic liquid-based colorimetric membrane/photonic crystal hybrid structure Reviewed

    Daiki Kawasaki, Ryoutarou Oishi, Nao Kobayashi, Tatsumi Mizuta, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Scientific Reports 雑誌   10   2020.08

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  • Size Sorting of Exosomes by Tuning the Thicknesses of the Electric Double Layers on a Micro-Nanofluidic Device.

    Satoko Fujiwara, Kyojiro Morikawa, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi

    Micromachines   11 ( 5 )   2020.04

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Exosomes, a type of extracellular vesicle with a diameter of 30-150 nm, perform key biological functions such as intercellular communication. Recently, size sorting of exosomes has received increasing attention in order to clarify the correlation between their size and components. However, such sorting remains extremely difficult. Here, we propose to sort their size by controlling their electrokinetic migration in nanochannels in a micro-nanofluidic device, which is achieved by tuning the thickness of the electric double layers in the nanochannels. This approach was demonstrated experimentally for exosomes smaller than 250 nm. Using different running buffer concentrations (1 × 10-3, 1 × 10-4, and 1 × 10-5 M), most of the exosomes larger than 140, 110, and 80 nm were successfully cut off at the downstream of the nanochannels, respectively. Therefore, it is clarified that the proposed method is applicable for the size sorting of exosomes.

    DOI: 10.3390/mi11050458

    PubMed

  • Enzyme-responsive Fluorescent Ionic Liquid Reviewed

    Ryoutarou OISHI, Tatsumi MIZUTA, Kenji SUEYOSHI, Tatsuro ENDO, Hideaki HISAMOTO

    Analytical Sciences 雑誌   36 ( 2 )   2020.02

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  • Enzyme-responsive Fluorescent Ionic Liquid Reviewed

    Oishi, Ryoutarou, Mizuta, Tatsumi, Sueyoshi, Kenji, Endo, Tatsuro, Hisamoto, Hideaki

    ANALYTICAL SCIENCES   36 ( 2 )   143 - 145   2020.02( ISSN:0910-6340

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:Domestic journal  

    Herein, we describe the development of a novel material, "enzyme-responsive fluorescent ionic liquid", which enabled a highly sensitive detection of alkaline phosphatase (ALP). We prepared a plasticized poly(vinyl chloride) (PVC) membrane using this new material as a plasticizer and quantified ALP in aqueous solutions. Preliminary results suggested that the PVC membrane responded to ALP at an interface between the membrane and the sample solution with anion extraction to maintain electroneutrality in the membrane phase. The developed PVC membrane showed an approximately six-times higher sensitivity than the conventional membrane, thereby demonstrating highly sensitive ALP detection. These results suggested the potential applicability of the proposed membrane for highly sensitive protein detection by using ALP-labeled antibodies.

    DOI: 10.2116/analsci.19C013

    PubMed

  • Size sorting of exosomes by tuning the thicknesses of the electric double layers on a micro-nanofluidic device Reviewed

    S. Fujiwara, K. Morikawa, T. Endo, H. Hisamoto, K. Sueyoshi

    Micromachines   11 ( 5 )   2020

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    Kind of work:Joint Work  

  • A lipophilic ionic liquid-based dye for anion optodes: importance of dye lipophilicity and application to heparin measurement Reviewed

    Tatsumi Mizuta, Shu Takai, Toshiki Nishihata, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Analyst 雑誌   145   2020

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    Kind of work:Joint Work  

  • Inkjet Printing-Based Immobilization Method for a Single-Step and Homogeneous Competitive Immunoassay in Microchannel Arrays Reviewed

    Yuko Kawai, Akihiro Shirai, Masaya Kakuta, Koutarou Idegami, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Frontiers in Chemistry 雑誌   8   2020

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  • Rapid, simple, sensitive, and multiple enzyme activity assay using a reagent-release capillary-assembled microdevice with double sweeping

    Kenji Sueyoshi, Ryota Sanuki, Tatsuro Endo, Hideaki Hisamoto

    21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017   1291 - 1292   2020

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    Publishing type:Research paper (international conference proceedings)  

    © 17CBMS-0001. In this study, we developed a novel microfluidic platform combining reagent-release capillaries and hydrogels (RRCs and RRGs) for multiple enzyme activity assay. Developed RRC-assembled device provided a rapid, simple and multiple enzyme activity assay by only introducing a sample solution by capillary action. The enzyme reaction products were then concentrated on the basis of double sweeping using both the cationic and anionic micelles from the RRGs by applying voltage. As a result, a rapid, simple, sensitive, and simultaneous assay of caspase-3, trypsin, and alkaline phosphatase was successfully realized by the developed device.

  • Inkjet Printing-Based Immobilization Method for a Single-Step and Homogeneous Competitive Immunoassay in Microchannel Arrays.

    Yuko Kawai, Akihiro Shirai, Masaya Kakuta, Kotaro Idegami, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Frontiers in chemistry   8   612132 - 612132   2020

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    In this study, we report an inkjet printing-based method for the immobilization of different reactive analytical reagents on a single microchannel for a single-step and homogeneous solution-based competitive immunoassay. The immunoassay microdevice is composed of a poly(dimethylsiloxane) microchannel that is patterned using inkjet printing by two types of reactive reagents as dissolvable spots, namely, antibody-immobilized graphene oxide and a fluorescently labeled antigen. Since nanoliter-sized droplets of the reagents could be accurately and position-selectively spotted on the microchannel, different reactive reagents were simultaneously immobilized onto the same microchannel, which was difficult to achieve in previously reported capillary-based single-step bioassay devices. In the present study, the positions of the reagent spots and amount of reagent matrix were investigated to demonstrate the stable and reproducible immobilization and a uniform dissolution. Finally, a preliminary application to a single-step immunoassay of C-reactive protein was demonstrated as a proof of concept.

    DOI: 10.3389/fchem.2020.612132

    PubMed

  • Imprinted photonic crystal nanocavity for p-spectrometer

    Kenichi Maeno, Shoma Aki, Kazuo Satoh, Shuichi Murakami, Yusuke Sando, Yusuke Kanaoka, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017   561 - 562   2020

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    Publishing type:Research paper (international conference proceedings)  

    © 17CBMS-0001. In this study, novel concept of micrometer-scale spectrometer (P-spectrometer) composed of imprinted photonic crystal nanocavities (PCNs) was proposed. The P-spectrometer has sufficient size to integrate into microfluidic devices and possible to detect light absorption high sensitively. Here we fabricated a PCN as unit cell of P-spectrometer using nanoimprint lithography, and experimentally confirmed for the first time that the fabricated PCN worked as a detection module for 560 nm wavelength.

  • Design of a PDMS microchannel array-based fast and single-step immunoassay principle using hydrophilic polymer-coated graphene oxide possessing multiple functions

    Akihiro Shirai, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017   1297 - 1298   2020

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    Publishing type:Research paper (international conference proceedings)  

    © 17CBMS-0001. This paper reports the preparation of the polyethylene glycol-coated graphene oxide (PEG-GO) possessing highly dispersive, fluorescence quenching and size separation functions, and its application to “single-step” immunoassay. The present immunoassay microdevice was fabricated by combining PDMS microchannel arrays separately immobilizing PEG-GO and fluorescently-labelled antibody as soluble coatings. When a sample solution was introduced into the microdevice by capillary action, all the reagents spontaneously mixed, reacted, and free fluorescently-labelled antibody was penetrated into the PEG structure and effectively quenched. Thus the antigen was successfully detected by single-step operation from the change of fluorescence intensity.

  • An ionic liquid composed of purely functional sensing molecules: a colorimetrically calcium responsive ionic liquid Reviewed

    Niwa, Yusuke, Mizuta, Tatsumi, Sueyoshi, Kenji, Endo, Tatsuro, Hisamoto, Hideaki

    ANALYST   144 ( 23 )   6858 - 6861   2019.12( ISSN:0003-2654

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    A highly lipophilic ionic liquid (IL) consisting of stoichiometrically equal amounts of two purely functional chemical sensing molecules, an anionic calcium ionophore and a cationic dye, was synthesized for the first time, and used as a component for a poly (vinyl chloride)-based or neat liquid membrane optical sensor. This is the first report of an IL consisting of purely functional chemical sensing molecules, which is completely different from the previously reported ionic liquids typically consisting of imidazolium or other lipophilic cations. Since the present IL contained an extremely high concentration of dyes, the IL-based sensor showed dramatically enhanced sensitivity (13 times higher compared to that of a conventional sensor), and fully reversible and selective response to Ca2+. Preliminary investigation on the unique response characteristics of the present liquid membrane IL-based sensor was performed with the responses to Ca2+ and Na+.

    DOI: 10.1039/c9an01769k

    PubMed

  • TiN-contained polymer-metal core-shell structured nanocone array: Engineering of sensor performance by controlling plasmonic properties Reviewed

    Kawasaki, Daiki, Maeno, Kenichi, Yamada, Hirotaka, Sueyoshi, Kenji, Hisamoto, Hideaki, Endo, Tatsuro

    SENSORS AND ACTUATORS B-CHEMICAL   299   2019.11

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    Publishing type:Research paper (scientific journal)  

    Metal nanostructures have great potential for optical label-free biosensors based on localized surface plasmon resonance (LSPR). The sensitivity of a metal nanostructure-based label-free biosensor (i.e., plasmonic sensor) depends on its plasmonic properties, which suffer a decrease in sensitivity by energy losses in the metal material. Here, we demonstrate an approach to improve the plasmonic properties of metal nanostructures by controlling the carrier density in the base polymer material using titanium nitride (TiN). It is expected that the light energy absorbed by TiN is converted into excitons, and it will assist LSPs in the metal nanostructure; thus, the losses of the metal material are compensated by the excitons excited in TiN. We designed a TiN-contained polymer-metal/core-shell structured nanocone array (NCA), comprising TiN nanoparticles (NPs) in a polymer core and metal shell (Au or Ag), and realized improvement of the refractive index (RI) sensitivity of a label-free biosensor by optimizing the TiN-contained polymer composition. As a result, the TiN-contained polymer-metal NCA, with a TiN NP concentration of 10 wt% in the polymer core, had a 1.5-fold higher RI sensitivity than that of the same NCA without TiN NPs. The results of the resistance measurement of the metal surface with the TiN NP-contained polymer (10 wt%) under light exposure suggest the conversion of exposed light into LSPs of metal via TiN. It is suggested that plasmonic properties and sensor performances can be improved by the presented approach. Moreover, in DNA hybridization detection, an extremely low limit of detection of 117.5 fM was achieved.

    DOI: 10.1016/j.snb.2019.126932

  • On-line sample preconcentration by polarity switching in floating electrode-integrated microchannel. Reviewed

    Kitagawa F, Tanigawa-Joh K, Terashita S, Fujiki R, Nukatsuka I, Sueyoshi K, Otsuka K

    Electrophoresis   40 ( 18-19 )   2478 - 2483   2019.09( ISSN:0173-0835

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    DOI: 10.1002/elps.201800501

    PubMed

  • Core-Shell-Structured Gold Nanocone Array for Label-Free DNA Sensing Reviewed

    Kawasaki, Daiki, Yamada, Hirotaka, Maeno, Kenichi, Sueyoshi, Kenji, Hisamoto, Hideaki, Endo, Tatsuro

    ACS APPLIED NANO MATERIALS   2 ( 8 )   4983 - 4990   2019.08( ISSN:2574-0970

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    Publishing type:Research paper (scientific journal)  

    The refractive index sensitivity of a localized surface plasmon resonance (LSPR) sensor is correlated to an enhanced local electromagnetic (EM) field originating from noble metal nanostructures. Here, we demonstrated that extensive EM field enhancement by a gold (Au) nanocone array (AuNCA) allowed highly sensitive and label-free detection of biomolecules in the visible wavelength spectrum. The AuNCA consisted of a polymer core and an Au shell, which was fabricated by using simple and inexpensive nanoimprint lithography. Under LSPR excitation, AuNCA absorbs visible light of a specific wavelength and extensively enhances the EM field near its surface. It was shown that AuNCA had high refractive index sensitivity (417.5 nm/RIU) because of the large distribution of the enhanced EM field, covering a large surface of the NCA. Moreover, in DNA hybridization detection, a very low limit of detection of 161 fM was achieved, and 1-base mismatch DNA was successfully discriminated by using AuNCA.

    DOI: 10.1021/acsanm.9b00930

  • Graphene/polyethylene glycol hybrids for single-step immunoassay microdevice Reviewed

    Shirai, Akihiro, Sueyoshi, Kenji, Endo, Tatsuro, Hisamoto, Hideaki

    FLATCHEM   13   34 - 39   2019.01( ISSN:2452-2627

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    Publishing type:Research paper (scientific journal)  

    In this study, we developed a novel particle-type material comprised of graphene and polyethylene glycol (PEG) to solve the slow response problem of the previously reported microdevice for single-step immunoassay. Characterization of these graphene/PEG hybrids (GPH) was carried out using a transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, and fluorescence analysis. We found that PEG attached to graphene surfaces behave like molecular sieves to separate free fluorescently-labeled antibodies from antigen-bound antibody immunocomplexes. The free antibody is PEG-permeable and directly adsorbs onto the graphene surface, where fluorescence is quenched by fluorescence resonance energy transfer. On the contrary, immunocomplexes are PEG-impermeable; thus, as the concentration of the antigen increases, the concentration of PEG-impermeable immunocomplexes also increases; consequently, increasing the fluorescence intensity of the solution. After characterizing GPH, single-step immunoassay microdevices were prepared by separately immobilizing the GPH and fluorescently-labeled antibodies on two independent microchannel arrays and combining them. Finally, we tested the proposed immunoassay microdevices with various known concentrations of C-reactive protein (CRP), an inflammatory marker protein, and successfully measured CRP concentration within an approximate time of 2 min by monitoring the fluorescence intensity change.

    DOI: 10.1016/j.flatc.2018.12.003

  • Graphene/polyethylene glycol hybrids for single-step immunoassay microdevice Reviewed

    A. Shirai, K. Sueyoshi, T. Endo, H. Hisamoto

    FlatChem 雑誌   2019

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  • TiN-contained polymer-metal core-shell structured nanocone array: Engineering of sensor performance by controlling plasmonic properties Reviewed

    Daiki Kawasaki, Kenichi Maeno, Hirotaka Yamada, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Sensors and Actuators B: Chemical 雑誌   2019

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    Kind of work:Joint Work  

  • Core–Shell-Structured Gold Nanocone Array for Label-Free DNA Sensing Reviewed

    Daiki Kawasaki, Hirotaka Yamada, Kenichi Maeno, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    ACS Applied Nano Materials 雑誌   2019

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  • Au "Edged Hole Array" for Sensor Application

    Hirotaka Yamada, Daiki Kawasaki, Chigusa Inoue, Kenichi Maeno, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Journal of Photopolymer Science and Technology   32 ( 1 )   2019

  • Development of a Rapid and Highly Sensitive Plasticized PVC Membrane Optode Utilizing an Ionic Liquid Material Composed of Bromothymol Blue

    Tatsumi MIZUTA, Kenji SUEYOSHI, Tatsuro ENDO, Hideaki HISAMOTO

    BUNSEKI KAGAKU   68 ( 12 )   2019

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  • An ionic liquid composed of purely functional sensing molecules: a colorimetrically calcium responsive ionic liquid

    Yusuke Niwa, Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Analyst   144   2019

  • Development of a Rapid and Highly Sensitive Plasticized PVC Membrane Optode Utilizing an Ionic Liquid Material Composed of Bromothymol Blue

    MIZUTA Tatsumi, SUEYOSHI Kenji, ENDO Tatsuro, HISAMOTO Hideaki

    BUNSEKI KAGAKU   68 ( 12 )   945 - 951   2019( ISSN:0525-1931

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    <p>In this work, ionic liquid materials composed of pH indicator dyes were synthesized and used as a plasticizer for a plasticized PVC membrane-based ion-selective optode. We chose several triphenylmethane dyes and synthesized ionic liquids with those by an ion-exchange procedure, and found that an ionic liquid composed of bromothymol blue (BTB) realized the highest ion-exchange ratio of approximately 100 % (highest concentration of dye). The absorbance response of the PVC membrane using a BTB-based ionic liquid was decreased by ca. 37 % using the repetitive introduction of acid and base solutions, and reached to exhibit a stable and reproducible response. The concentration of dye was 20∼70-times higher than that of the conventional ones. In addition, the PVC membrane was applied for anion sensing based on the coextraction of protons and anions in aqueous solutions, and it was found that the ion selectivity followed the Hofmeister series. In the future, the present ionic liquid material is expected to be applied for highly-selective and sensitive ion-selective optodes by the modification of functional groups such as ion-recognition or reaction with the target molecule.</p>

    DOI: 10.2116/bunsekikagaku.68.945

    CiNii Article

  • Au "Edged Hole Array" for Sensor Application Reviewed

    Yamada, Hirotaka, Kawasaki, Daiki, Inoue, Chigusa, Maeno, Kenichi, Sueyoshi, Kenji, Hisamoto, Hideaki, Endo, Tatsuro

    JOURNAL OF PHOTOPOLYMER SCIENCE AND TECHNOLOGY   32 ( 1 )   101 - 105   2019( ISSN:0914-9244

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    Publishing type:Research paper (scientific journal)  

    This paper described the design and fabrication of precursor for Au hole structure with four edges (edged hole array: EHA) for sensor application. In this paper, effect of the gap length of opposite edges g and hole side length l was investigated systematically by simulation. As the result, optimal design of EHA that shows strong light absorption (> 90%) and electric field (1.79x10(5)-fold increase against the incident light) at lambda = 785 nm was revealed. Finally, the precursor resist pattern for EHA was fabricated using electron beam lithography and cold development. As the result, the precursor structure that was expected to generate strong electric field at lambda = 785 nm was successfully fabricated.

    DOI: 10.2494/photopolymer.32.101

  • A multi-dimensional fractionation microdevice based on digital electrophoresis using convex-concave cartridges Reviewed

    Y. Uwagawa, T. Endo, H. Hisamoto, K. Sueyoshi

    The Proceedings of the MicroTAS 2018 Conference 雑誌   2018

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  • Functionalized graphene nanoparticle toword rapidly measurable and easy-to-use immunoassay microdevice Reviewed

    A. Shirai, K. Sueyoshi, T. Endo, H. Hisamoto

    The Proceedings of the MicroTAS 2018 Conference 雑誌   2018

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    Kind of work:Joint Work  

  • Position-selective immobilization of sensor membranes on convex-shaped PDMS chip for naked evy-based multiplexed detection Reviewed

    T. Mizuta, K. Maeno, K. Sueyoshi, T. Endo, H. Hisamoto

    The Proceedings of the MicroTAS 2018 Conference 雑誌   2018

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  • Calcium-selective “dyed plasticizer” on PDMS microchip: Rapid and highl sensitive naked eye-based quantitation of calcium ion Reviewed

    Y. Niwa, T. Mizuta, K. Sueyoshi, T. Endo, H. Hisamoto

    The Proceedings of the MicroTAS 2018 Conference 雑誌   2018

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    Kind of work:Joint Work  

  • "Dyed plasticizer": Application to rapid and highly sensitive heparin detection based on PDMS microchannel array devices Reviewed

    T. Nishihata, T. Mizuta, K. Sueyoshi, T. Endo, H. Hisamoto

    The Proceedings of the MicroTAS 2018 Conference 雑誌   2018

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  • Development of a polymer/TiO2 hybrid two-dimensional photonic crystal for highly sensitive fluorescence-based ion sensing applications Reviewed

    S. Aki, K. Maeno, K. Sueyoshi, H. Hisamoto, T. Endo

    Sensors and Actuators B: Chemical 雑誌   2018

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  • Design and Fabrication of a Visible-Light-Compatible, Polymer-Based Photonic Crystal Resonator and Waveguide for Sensing Applications Reviewed

    J. Sun, K. Maeno, S. Aki, K. Sueyoshi, H. Hisamoto, T. Endo

    Micromachines 雑誌   2018

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    Kind of work:Joint Work  

  • Regioselective Immobilization of a PVC Membrane Composed of an Ionic Liquid-based Dye on Convex-shaped PDMS Surface for Multiplexed Microanalytical Devices Reviewed

    T. Mizuta, K. Maeno, K. Sueyoshi, T. Endo, H. Hisamoto

    Analytical Sciences 雑誌   2018

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    Kind of work:Joint Work  

  • Ionic liquid-based dye: A “Dyed plasticizer” for rapid and highly sensitive anion optodes based on a plasticized PVC membrane Reviewed

    T. Mizuta, K. Sueyoshi, T. Endo, H. Hisamoto

    Sensors and Actuators B: Chemical 雑誌   2018

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    Kind of work:Joint Work  

  • Design of a PDMS microchannel array-based fast and singlestep immunoassay principle using hydrophilic polymer-coated graphene oxide possessing multiple functions Reviewed

    Akihiro Shirai, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    The Proceedings of the MicroTAS 2017 Conference 雑誌   2017

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  • Rapid, simple, sensitive, and multiple enzyme activity assay using a reagent-release capillary-assembled microdevice with double sweeping Reviewed

    Kenji Sueyoshi, Ryota Sanuki, Tatsuro Endo, Hideaki Hisamoto

    The Proceedings of the MicroTAS 2017 Conference 雑誌   2017

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  • Imprinted photonic crystal nanocavity for μ-spectrometer Reviewed

    Kenichi Maeno, Shoma Aki, Kazuo Satoh, Shuichi Murakami, Yusuke Sando, Yusuke Kanaoka, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    The Proceedings of the MicroTAS 2017 Conference 雑誌   2017

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  • A “dyed plasticizer” for rapid and highly sensitive sensing based on a plasticized PVC membrane for capillary array-based microanalytical devices Reviewed

    Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    The Proceedings of the MicroTAS 2017 Conference 雑誌   2017

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  • A Simple and Easy-to-Use Capillary Isoelectric Focusing Technique Using Reagent-Release Hydrogels Reviewed

    Yuji Fujii, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Chromatography 雑誌   2017

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  • Combination of large-volume sample stacking with an electroosmotic flow pump with field-amplified sample injection on cross-channel chips Reviewed

    Fumihiko Kitagawa, Tatsuya Ishiguro, Misaki Tateyama, Isoshi Nukatsuka, Kenji Sueyoshi, Takayuki Kawai, Koji Otsuka

    Electrohoresis 雑誌   2017

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  • Development of Element Technology for 1 STEP Biomarker Protein Analysis Device Using Silver Nanoparticl-Contained Hydrogel and Reagent-Immobilized Cartridge Reviewed

    Kenzo Yamamoto, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Electron. Commun. Jpn, 雑誌   2017

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    Kind of work:Joint Work  

  • Origin of the Optical Response of a Dye-doped Plasticized Poly(vinyl chloride)-based Photonic Crystal Ion Sensor Reviewed

    Shoma Aki, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Analytical Sciences 雑誌   2017

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  • Fast and Single-step Fluorescence-based Competitive Bioassay Microdevice Combined PDMS Microchannel Arrays Separately Immobilizing Graphene Oxide–Analyte Conjugates and Fluorescently-labelled Receptor Proteins Reviewed

    Akihiro Shirai, Kaho Nakashima, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Analytical Sciences 雑誌   2017

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  • Development of Cartridge-Based Wash-Free Single-Step Plasmonic Enzyme-Linked Immunosorbent Assay Using Poly(vinylpyrrolidinone)-Coated Silver Nanoparticles as a Chromogenic Substrate. Reviewed

    Tatsuro Endo, Kenzo Yamamoto, Kenji Sueyoshi, Hideaki Hisamoto

    Sensors and Materials 雑誌   2017

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  • Double Sweeping: Highly Effective Sample Preconcentration Using Cationic and Anionic Micelles and Its Application to a Multiple Enzyme Activity Assay Reviewed

    Ryota Sanuki, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Analytical Chemistry 雑誌   2017

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    Kind of work:Joint Work  

  • Development of a single-step immunoassay microdevice based on a graphene oxide-containing hydrogel possessing fluorescence quenching and size separation functions Reviewed

    Akihiro Shirai, Kaho Nakashima, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Analyst 雑誌   2017

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  • On-line coupling of sample preconcentration by LVSEP with gel electrophoretic separation on T-channel chips Reviewed

    Fumihiko Kitagawa, Saeko Kinami, Yuuki Takegawa, Isoshi Nukatsuka, Kenji Sueyoshi, Takayuki Kawai, Koji Otsuka

    Electrophoresis 雑誌   2017

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  • Single-step immunoassay microdevice based on graphene oxide-containing hydrogel possessing fluorescence quenching and size separation functions Reviewed

    Akihiro Shirai, Kaho Nakashima, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    The Proceedings of the MicroTAS 2016 Conference 雑誌   2016

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  • Development of a designable digital electrophoresis device by connecting capillary cartridges filled with a different functionalized hydrogel Reviewed

    Yuta Aoki, Tadamasa Kanaoka, Keita Matsuda, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    The Proceedings of the MicroTAS 2016 Conference 雑誌   2016

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    Kind of work:Joint Work  

  • Fabrication of gold-deposited plasmonic crystal based on nanoimprint lithography for label-free biosensing application Reviewed

    Kiichi Nishiguchi, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Jpn. J. Appl. Phys. 雑誌   2016

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    Kind of work:Joint Work  

  • Development of optical biosensor based on photonic crystal made of TiO2 using liquid phase deposition Reviewed

    Keigo Aono, Shoma Aki, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Jpn. J. Appl. Phys. 雑誌   2016

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    Kind of work:Joint Work  

  • Fast and single-step immunoassay based on fluorescence quenching within a square glass capillary immobilizing graphene oxide–antibody conjugate and fluorescently labelled antibody Reviewed

    Akihiro Shirai, Terence Henares, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Analyst 雑誌   2016

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    Kind of work:Joint Work  

  • Polymer-based Photonic Crystal Cavity Sensor for Optical Detection in the Visible Wavelength Region Reviewed

    K. Maeno, S. Aki, K. Sueyoshi, H. Hisamoto, T. Endo

    Analytical Sciences 雑誌   2016

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    Kind of work:Joint Work  

  • Highly Sensitive and Multiple Enzyme Activity Assay Using Reagent-release Capillary– Isoelectric Focusing with Rhodamine 110-based Substrates Reviewed

    K. Sueyoshi, Y. Nogawa, K. Sugawara, T. Endo, H. Hisamoto

    Analytical Sciences 雑誌   2016

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  • A single-step enzyme immunoassay capillary sensor composed of functional multilayer coatings for diagnosis marker proteins Reviewed

    Shun-ichi Funano, Masato Sugahara, Terence Henares, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Analyst 雑誌   2015

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  • Highly Sensitive Enzyme Activity Assay Microdevice Based on Isoelectric Focusing Using Bifunctional Fluorescent Substrates and Reagent-release Hydrogels Reviewed

    K. Sugawara, K. Sueyoshi, T. Endo, H. Hisamoto

    The Proceedings of the MicroTAS 2015 Conference 雑誌   2015

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  • Double Sweeping Using Reagent-Release Hydrogels for A Highly Sensitive Electrophoretic Bioassay Microdevice Reviewed

    R. Sanuki, K. Sueyoshi, T. Endo, H. Hisamoto

    The Proceedings of the MicroTAS 2015 Conference 雑誌   2015

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  • Rapid and Highly Sensitive Immunoassay Device Based on Electrophoretic Filtration Using A Hydrogel Immobilizing Fluorescent Substrates Reviewed

    S. Miyamoto, K. Sueyoshi, T. Endo, H. Hisamoto

    The Proceedings of the MicroTAS 2015 Conference 雑誌   2015

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  • Rapid and Sensitive Enzyme Activity Assay Microdevice by Electrophoretic Filtration and A Hydrogel Immobilizing Fluorescent Substrates Reviewed

    T. Nishiwaki, S. Miyamoto, K. Sueyoshi, T. Endo, H. Hisamoto

    The Proceedings of the MicroTAS 2015 Conference 雑誌   2015

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  • Mass-producible Capillary-assembled Microchip (CAS-CHIP) for Multiple Sensing with Single-step Operation Toward Point of Care Testing Reviewed

    A. Shirai, T. G. Henares, K. Sueyoshi, T. Endo, H. Hisamoto

    The Proceedings of the MicroTAS 2015 Conference 雑誌   2015

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  • A Simple and Rapid Immunoassay Based on Microchip Electrophoresis Using a Reagent- Release Cartridge Reviewed

    K. Sueyoshi, Y. Miyahara, T. Endo, H. Hisamoto

    Chromatography 雑誌   2015

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  • Simple and Rapid Immobilization of Coating Polymers on Poly(dimethyl siloxane)-glass Hybrid Microchips by a Vacuum-drying Method Reviewed

    F. Kitagawa, S. Nakagawara, I. Nukatsuka, Y. Hori, K. Sueyoshi, K. Otsuka

    Analytical Sciences 雑誌   2015

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    Kind of work:Joint Work  

  • Development of element technology for 1 STEP biomarker protein analysis device using silver nanoparticle contained hydrogel and reagent immobilized cartridge Reviewed

    K. Yamamoto, K. Sueyoshi, H. Hisamoto, T. Endo

    IEEJ Transactions on Electronics, Information and Systems 雑誌   2015

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    Kind of work:Joint Work  

  • Development of Microchip Electrophoresis -Integrated Nanoimprinted Photonic Crystal Reviewed

    T. Endo, K. Yamamoto, K. Sueyoshi, H. Hisamoto

    Sensors and Materials 雑誌   2015

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  • Fabrication and packaging of a mass- producible capillary-assembled microchip for simple and multiplexed bioassay Reviewed

    T. Henares, A. Shirai, K. Sueyoshi, T. Endo, H. Hisamoto

    Sensors and Actuators B 雑誌   2015

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    Kind of work:Joint Work  

  • Simple and Effective Label-Free Capillary Electrophoretic Analysis of Sugars by Complexation Using Quinoline Boronic Acids Reviewed

    T. Kubo, K. Kanemori, R. Kusumoto, T. Kawai, K. Sueyoshi, T. Naito, K. Otsuka

    Analytical Chemistry 雑誌   2015

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    Kind of work:Joint Work  

  • Polymer/TiO2 hybrid two-dimensional photonic crystal for enhanced fluorescence detection Reviewed

    Shoma Aki, Tatsuro Endo, Kenji Sueyoshi, Hideaki Hisamoto

    The Proceedings of the MicroTAS 2014 Conference 雑誌   2014

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  • Preparation of hydrogel immobilizing fluorescent substrate on a flexible polymer sheet amd its application to mass-producible and single-step multi-sensing device Reviewed

    Seiji Odaka, Kaede Jigawa, Shun-ichi Funano, Terence Henares, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    The Proceedings of the MicroTAS 2014 Conference 雑誌   2014

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  • Two-dimensional digital electrophoresis of proteins using mosaic hydrogel Reviewed

    Tadamasa Kanaoka, Keita Matsuda, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    The Proceedings of the MicroTAS 2014 Conference 雑誌   2014

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  • Ultrafast and single-step immunoassay using fanctional graphene oxide for capillary-assembled microchip Reviewed

    Akihiro Shirai, Terence Henares, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    The Proceedings of the MicroTAS 2014 Conference 雑誌   2014

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  • Polymer based photonic crystal cavity for highly sensitive optical detection Reviewed

    Kenichi Maeno, Shoma Aki, Tatsuro Endo, Kenji Sueyoshi, Hideaki Hisamoto

    The Proceedings of the MicroTAS 2014 Conference 雑誌   2014

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  • Evaluation of the stacked functional hydrogels toword microfluidic western blotting based on multi-dimensional digital electrophoresis using capillary-assembled microchip Reviewed

    Keita Matsuda, Tadamasa Kanaoka, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    The Proceedings of the MicroTAS 2014 Conference 雑誌   2014

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  • Hydrogel based 2D-photonic crystal including acrylic acid for biosencing application Reviewed

    Yoko Matsumoto, Takashi Araki, Tatsuro Endo, Kenji Sueyoshi, Hideaki Hisamoto

    The Proceedings of the MicroTAS 2014 Conference 雑誌   2014

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  • Rapid and highly sensitive electrophoretic immunoassay device based on the on-line concentration of enzyme-labeled antibody using hydrogel immobilizing fluorescent substrate Reviewed

    Shogo Miyamoto, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    The Proceedings of the MicroTAS 2014 Conference 雑誌   2014

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  • Hydrophilic Interaction Electrokinetic Chromatography Using Bio-based Nanofillers Reviewed

    Kawai, T.; Watanabe, M.; Uetani, K.; Fukushima, Y.; Sueyoshi, K.; Kubo, T.; Kitagawa, F.; Yano, H.; Otsuka, K.

    Electrophoresis 雑誌   2014

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  • Quantitative Ligand Immobilization Using Alginate Hydrogel Formed in a Capillary: Application for On-line Affinity Concentration Reviewed

    Fukushima, Y.; Naito, T.; Sueyoshi, K.; Kubo, T.; Kitagawa, F.; Otsuka, K.

    Analytical Chemistry 雑誌   2014

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  • Sensitivity Enhancement by Sweeping via Borate Complexation in Capillary Electrophoretic Analysis of Glycoproteins Reviewed

    Kitagawa, F.; Nojiri, M.; Sueyoshi, K.; Otsuka, K.

    Chromatography 雑誌   2014

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  • Plasticized poly(vinyl chloride)-based photonic crystal for ion sensing Reviewed

    Shoma Aki, Tatsuro Endo, Kenji Sueyoshi, Hideaki Hisamoto

    Analytical Chemistry 雑誌   2014

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  • Development of novel protease assay device using a nanoimprinted two dimensional photonic crystal Reviewed

    Wakana Hashimoto, Tatsuro Endo, Kenji Sueyoshi, Hideaki Hisamoto

    Chem. Lett. 雑誌   2014

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  • Efficient immobilization of the enzyme and substrate for a single-step caspase-3 inhibitor assay using a combinable PDMS capillary sensor array Reviewed

    Tadashi Ishimoto, Kaede Jigawa, Terence Henares, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    RSC Advances 雑誌   2014

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  • Development of a C60-fullerene bonded open-tubular capillary using a photo/thermal active agent for liquid chromatographic separations by π–π interactions Reviewed

    Takuya Kubo, Yoshiki Murakami, Yuichi Tominaga, Toyohiro Naito, Kenji Sueyoshi, Mingdi Yan, Koji Otsuka

    Journal of Chromatography A 雑誌   2014

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  • Effective determination of a pharmaceutical, sulpiride, in river water by online SPE-LC–MS using a molecularly imprinted polymer as a preconcentration medium Reviewed

    Takuya Kubo, Kenta Kuroda, Yuichi Tominaga, Toyohiro Naito, Kenji Sueyoshi, Ken Hosoya, Koji Otsuka

    Journal of Pharmaceutical and Biomedical Analysis 雑誌   2014

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  • Plasticized PVC-based photonic crystal for ion sensing application Reviewed

    Shoma Aki, Tatsuro Endo, Kenji Sueyoshi, Hideaki Hisamoto

    The Proceedings of the MicroTAS 2013 Conference 雑誌   2013

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  • Fabrication of hydrogel-based tow-dimensional photonic crystal for optical sensor appilcation Reviewed

    Takashi Araki, Tatsuro Endo, Kenji Sueyoshi, Hideaki Hisamoto

    The Proceedings of the MicroTAS 2013 Conference 雑誌   2013

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  • Development of novel label-free enzyme activity assay using nanoimprinted ohtoniccrystal for urokinase activity measurement Reviewed

    Wakana Hashimoto, Tatsuro Endo, Kenji Sueyoshi, Hideaki Hisamoto

    The Proceedings of the MicroTAS 2013 Conference 雑誌   2013

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  • Single-step Caspase-3 Inhibitor Assay by Using Combinable PDMS Capillary (CPC) Sensor Array Reviewed

    Tadashi Ishimoto, Kaede Jigawa, Terence Henares, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    The Proceedings of the MicroTAS 2013 Conference 雑誌   2013

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  • Single-step Enzyme Immunoassay Using Lipophilic Fluorescent Substrate for Capillary-assembled Microchip Reviewed

    Masato Sugahara, Shun-ichi Funano, Terence Henares, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    The Proceedings of the MicroTAS 2013 Conference 雑誌   2013

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  • Flatbed Scanner-based Detection for Capillary-assembled Microchip Reviewed

    Shohei Kubo, Terence Henares, Shun-ichi Funano, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    The Proceedings of the MicroTAS 2013 Conference 雑誌   2013

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  • Development of Electrospray Ionization Interface-Integrated Microchip for Mass Spectrometric Detection in Electrophoresis Reviewed

    Mami Oketani, Takayuki Kawai, Toyohiro Naito, Kenji Sueyoshi, Takuya Kubo, Fumihiko Kitagawa, Koji Otsuka

    The Proceedings of the MicroTAS 2013 Conference 雑誌   2013

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  • Simple and Rapid Immunoassay Using Micro Isoelectric Focusing Device and Reagent Release Hydrogels Reviewed

    Yuji Fujii, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    The Proceedings of the MicroTAS 2013 Conference 雑誌   2013

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  • Simple and Highly-Sensitive Enzyme Activity Assay Microdevice Based on the Combination of Reagent Release Hydrogel and Capillary Array Reviewed

    Naoki Agura, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    The Proceedings of the MicroTAS 2013 Conference 雑誌   2013

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  • Development of Microfluidic Blotting Devices Using Alginate Hydrogel Reviewed

    Yudai Fukushima, Toyohiro Naito, Kenji Sueyoshi, Takuya Kubo, Koji Otsuka

    The Proceedings of the MicroTAS 2013 Conference 雑誌   2013

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  • Capillary-based Enzyme-linked Immunosorbent Assay for Highly Sensitive Detection of Thrombin-cleaved Osteopontin in Plasma Reviewed

    Shun-ichi Funano, Terence Henares, Mie Kurata, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Analytical Biochemistry 雑誌   2013

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  • Novel Fluorescent Probe for Highly-Sensitive Bioassay using Sequential Enzyme-Linked Immunosorbent Assay-Capillary Isoelectric Focusing (ELISA-cIEF) Reviewed

    Terence Henares, Yuta Uenoyama, Yuto Nogawa, Ken Ikegami, Daniel Citterio, Koji Suzuki, Shun-ichi Funano, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Analyst 雑誌   2013

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  • Design of Single-Step Immunoassay Principle Based on the Combination of Enzyme-Labeled Antibody Release Coating and Hydrogel Copolymerized with Fluorescent Enzyme Substrate in a Microfluidic Capillary Device Reviewed

    Hideki Wakayama, Terence Henares, Kaede Jigawa, Shun-ichi Funano, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Lab on a Chip 雑誌   2013

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  • Synthesis of Poly(ethylene glycol)-Based Hydrogels and Their Swelling/Shrinking Response to Molecular Recognition Reviewed

    Yuichi Tominaga, Takuya Kubo, Kenji Sueyoshi, Ken Hosoya, Koji Otsuka

    Journal of Polymer Science Part A: Polymer Chemistry 雑誌   2013

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  • Toward 10 000-fold sensitivity improvement of oligosaccharides in capillary electrophoresis using large-volume sample stacking with an electroosmotic flow pump combined with field-amplified sample injection Reviewed

    Takayuki Kawai, Masumi Ueda, Yudai Fukushima, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka

    Electrophoresis 雑誌   2013

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  • Effect of a Low-conductivity Zone on Field-amplified Sample Stacking in Microchip Micellar Electrokinetic Chromatography Reviewed

    Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka

    Analytical Sciences 雑誌   2013

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  • Open-tubular Electrochromatographic Chiral Separation of Amino Acids Using an Organic Nanocrystals Immobilized Capillary Reviewed

    Fumihiko Kitagawa, Hiroshi Sudaki, Kenji Sueyoshi, Koji Otsuka

    Analytical Sciences 雑誌   2013

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  • Zone electrophoresis of proteins in poly(dimethyl-siloxane) (PDMS) microchip coated with physically adsorbed amphiphilic phospholipid polymer Reviewed

    Kyosuke Nii, Kenji Sueyoshi, Koji Otsuka, Madoka Takai

    Microfluidics and Nanofluidics 雑誌   2013

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  • Inner surface modification of poly(dimethylsiloxane) microchannel with chitin for electrophoretic analysis of proteins Reviewed

    Kenji Sueyoshi, Yusuke Hori, Koji Otsuka

    Microfluidics and Nanofluidics 雑誌   2013

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  • PDMS microchip electrophoresis with high separation efficiency by simple and quick modification of phospholipid polymer Reviewed

    Kyosuke Nii, Kenji Sueyoshi, Koji Otsuka, Madoka Takai

    The Proceedings of the MicroTAS 2012 Conference 雑誌   2012

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  • Development of microfluidic blotting devices using alginate hydrogel Reviewed

    Masahiro Ikawa, Yudai Fukushima, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka

    The Proceedings of the MicroTAS 2012 Conference 雑誌   2012

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    Kind of work:Joint Work  

  • Sensitive enantioseparation by transient trapping-cyclodextrin electrokinetic chromatography Reviewed

    Sueyoshi, K.; Koino, H.; Kitagawa, F.; Otsuka, K.

    Journal of Chromatography A 雑誌   2012

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    Kind of work:Joint Work  

  • Electrophoretic analysis of cations using large-volume sample stacking with an electroosmotic flow pump using capillaries coated with neutral and cationic polymers Reviewed

    Kawai, T.; Ito, J.; Sueyoshi, K.; Kitagawa, F.; Otsuka, K.

    Journal of Chromatography A 雑誌   2012

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    Kind of work:Joint Work  

  • Highly sensitive chiral analysis in capillary electrophoresis with large-volume sample stacking with an electroosmotic flow pump Reviewed

    Kawai, T.; Koino, H.; Sueyoshi, K.; Kitagawa, F.; Otsuka, K.

    Journal of Chromatography A 雑誌   2012

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    Kind of work:Joint Work  

  • Highly sensitive oligosaccharide analysis in capillary electrophoresis using large-volume sample stacking with an electroosmotic flow pump Reviewed

    Kawai, T.; Watanabe, M.; Sueyoshi, K.; Kitagawa, F.; Otsuka, K.

    Journal of Chromatography A 雑誌   2012

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    Kind of work:Joint Work  

  • Recent progress of on-line combination of preconcentration device with microchip electrophoresis Reviewed

    Kenji Sueyoshi

    Chromatography 雑誌   2012

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    Kind of work:Joint Work  

  • Over 50,000-fold sample preconcentration efficiency in microchip elctrophoresis using a simple channel Reviewed

    Takayuki Kawai, Masumi Ueda, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka

    The Proceedings of the MicroTAS 2011 Conference 雑誌   2011

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    Kind of work:Joint Work  

  • Hydrophobic labeling of amino acids: Transient trapping−capillary / microchip electrophoresis Reviewed

    Sueyoshi, K.; Hashiba, K.; Kawai, T.; Kitagawa, F.; Otsuka, K.

    Electrophoresis 雑誌   2011

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    Kind of work:Joint Work  

  • One-step preparation of amino-PEG modified poly(methyl methacrylate) microchips for electrophoretic separation of biomolecules Reviewed

    Fumihiko Kitagawa, Kei Kubota, Kenji Sueyoshi, Koji Otsuka

    J. Pharm. Biomed. Anal. 雑誌   2010

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    Kind of work:Joint Work  

  • Microchip electrophoresis of oligosaccharides using large-volume sample stacking with an electroosmotic flow pump in a single channel Reviewed

    Takayuki Kawai, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka

    Analytical Chemistry 雑誌   2010

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    Kind of work:Joint Work  

  • On-line sample preconcentration and separation technique based on transient trapping in microchip micellar electrokinetic chromatography Reviewed

    Sueyoshi, Kenji; Kitagawa, Fumihiko, Otsuka, Koji

    Analytical Chemistry 雑誌   2008

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    Kind of work:Joint Work  

  • オンライン試料濃縮法及び部分的注入法を用いる高性能マイクロチップ電気泳動分析 Reviewed

    末吉健志, 北川文彦, 大塚浩二

    分析化学 雑誌   2008

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    Kind of work:Joint Work  

  • Application of partial filling technique to electrophoretic analysis on microchip with T-cross channel configuration Reviewed

    Sueyoshi, Kenji; Nagai, Hidenori; Wakida, Shin-ichi; Nishii, Junji; Kitagawa, Fumihiko; Otsuka, Koji

    Meas. Sci. Technol 雑誌   2006

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    Kind of work:Joint Work  

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Books and Other Publications

  • 機器分析ハンドブック 2 高分子・分離分析編

    床波 志保,前田 耕治,安川 智之,飯田 琢也,末吉 健志 他( Role: Joint author)

    化学同人  2020.10  ( ISBN:978-4-7598-2022-5

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    Responsible for pages:66-79  

MISC

  • Development of Capillary Devices for Digital Molecular Sieving Electrophoresis

    末吉健志, 末吉健志, 松田景太, 遠藤達郎, 久本秀明

    分析化学   71 ( 6 )   2022( ISSN:0525-1931

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  • Fabrication of Quantum Dots / TiO<sub>2</sub> Hybrid Photonic Crystal for Optical Biosensor Application in Visible Region

    植田渉太郎, 川崎大輝, 西辻凌輔, 山田大空, 末吉健志, 末吉健志, 久本秀明, 遠藤達郎

    応用物理学会春季学術講演会講演予稿集(CD-ROM)   69th   2022( ISSN:2436-7613

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  • Label-free detection of cell metabolites using photonic crystals

    WANG Yankang, 中島悠佑, 植田渉太郎, 末吉健志, 末吉健志, 久本秀明, 遠藤達郎

    応用物理学会春季学術講演会講演予稿集(CD-ROM)   69th   2022( ISSN:2436-7613

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  • Development of label-free PCR by using plasmonic crystal

    古川ふうた, 山田大空, 川崎大輝, 西辻凌輔, 遠藤達郎, 末吉健志, 末吉健志, 久本秀明

    応用物理学会春季学術講演会講演予稿集(CD-ROM)   69th   2022( ISSN:2436-7613

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  • Development of a Single-step Bioassay Microdevice Using a Reagent Immobilization Method Based on Inkjet Printing

    河相優子, 白井亮洋, 角田正也, 井手上公太郎, 末吉健志, 末吉健志, 遠藤達郎, 久本秀明

    分析化学   70 ( 3 )   2021( ISSN:0525-1931

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  • FRET機構を利用した超高感度カチオンセンシング用蛍光色素液体薄膜の開発

    西海豪祐, 水田巽, 末吉健志, 遠藤達郎, 久本秀明

    分析化学討論会講演要旨集(Web)   81st   2021

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  • フォトニック結晶を用いたDNAメチル化の光検出

    中島悠佑, 小林奈緒, 植田渉太郎, 川崎大輝, 末吉健志, 末吉健志, 久本秀明, 遠藤達郎

    化学とマイクロ・ナノシステム学会研究会講演要旨集   43rd (CD-ROM)   2021

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  • 多段階部分的注入アフィニティキャピラリー電気泳動に基づく核酸-低分子化合物間相互作用解析

    光野恵理子, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    分析化学討論会講演要旨集(Web)   81st   2021

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  • 低分子標的アプタマー選抜のためのキャピラリー分子ふるい電気泳動

    和田将英, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    分析化学討論会講演要旨集(Web)   81st   2021

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  • ミクロスケール電気泳動フィルタリングによる高効率アプタマー選抜法の開発

    上野楓, 高尾隼空, 飛田安梨沙, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    分析化学討論会講演要旨集(Web)   81st   2021

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  • キャピラリー分子ふるい電気泳動を利用した低分子標的アプタマー選抜法

    和田将英, 遠藤達郎, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    分析化学討論会講演要旨集(Web)   80th   2020

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  • キャピラリー分子ふるい電気泳動に基づく低分子標的アプタマー選抜法

    和田将英, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    日本分析化学会年会講演要旨集(Web)   69th   2020

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  • キャピラリー分子ふるい電気泳動による低分子標的核酸アプタマー選抜法

    和田将英, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    キャピラリー電気泳動シンポジウム講演要旨集   40th (CD-ROM)   2020

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  • A “dyed plasticizer” for rapid and highly sensitive sensing based on a plasticized PVC membrane for capillary array-based microanalytical devices

    T. Mizuta, K. Sueyoshi, T. Endo, H. Hisamoto

    21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017   521 - 522   2020

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    © 17CBMS-0001. In this work, we synthesized a new material, “dyed plasticizer”, [P66614]2[NP] which consists of two trihexyltetradecylphospohonium cations and a naphtholphthalein anion, and prepared the plasticized PVC membrane. We systematically investigated the reversibility of response, sensitivity and response time, and demonstrated rapid and highly-sensitive anion sensing based on the coextraction of anions and protons into the bulk of the plasticized PVC membrane. We prepared a microchannel array device combined with plasticized PVC membrane, and Cl- sensing using flat-bed scanner was successfully demonstrated.

  • プラズモニック結晶を用いた非染色PCR産物検出デバイスの開発

    古川ふう大, 井上千種, 川崎大輝, 山田大空, 末吉健志, 末吉健志, 久本秀明, 遠藤達郎

    日本分析化学会年会講演要旨集(Web)   69th   2020

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  • 高精度な細胞外微粒子分級用マイクロ・ナノ流体デバイスの開発

    藤原聡子, 金子紗也, 森川響二朗, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    日本分析化学会年会講演要旨集(Web)   69th   2020

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  • 高感度イオンセンシングを志向した色素液体ナノエマルションの創製

    牧佳穂, 大石綾太郎, 水田巽, 末吉健志, 遠藤達郎, 久本秀明

    日本分析化学会年会講演要旨集(Web)   69th   2020

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  • Evaluation of surface treatment conditions for reduction of non-specific adsorption on polymer-based photonic crystal

    中野萌, 山田大空, 川崎大輝, 末吉健志, 久本秀明, 遠藤達郎

    日本化学会春季年会講演予稿集(CD-ROM)   100th   2020

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  • 電気泳動フィルタリングによる標的分子捕捉とDNA結合・解離を利用した新規アプタマー選抜法の開発

    高尾隼空, 飛田安梨沙, 上野楓, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    キャピラリー電気泳動シンポジウム講演要旨集   40th (CD-ROM)   2020

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  • 蛍光性色素液体を用いた超高感度FRET検出型PVC膜オプトードの開発

    水田巽, 末吉健志, 遠藤達郎, 久本秀明

    分析化学討論会講演要旨集(Web)   80th   2020

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  • 蛍光性色素液体を用いたFRET検出型PVC膜オプトードの開発と超高感度イオンセンシングへの応用

    水田巽, 末吉健志, 遠藤達郎, 久本秀明

    日本分析化学会年会講演要旨集(Web)   69th   2020

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  • 色素液体ナノエマルションの創製と高感度イオンセンシングの基礎検討

    牧佳穂, 大石綾太郎, 水田巽, 末吉健志, 遠藤達郎, 久本秀明

    分析化学討論会講演要旨集(Web)   80th   2020

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  • 液状イオノフォアと固体色素に基づく高感度アンモニウムイオンセンシング液体開発の基礎検討

    岡嵩人, 水田巽, 末吉健志, 末吉健志, 遠藤達郎, 遠藤達郎, 久本秀明

    分析化学討論会講演要旨集(Web)   80th   2020

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  • 核酸アプタマー高効率選抜のためのミクロスケール電気泳動フィルタリングデバイス

    末吉健志, 末吉健志, 永井伶奈, 遠藤達郎, 久本秀明

    日本分析化学会年会講演要旨集(Web)   69th   2020

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  • 多段階部分的注入キャピラリー電気泳動法による核酸-低分子化合物間相互作用解析

    光野恵理子, 遠藤達郎, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    分析化学討論会講演要旨集(Web)   80th   2020

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  • 多段階部分的注入アフィニティーキャピラリー電気泳動法による疾患関連核酸-低分子化合物間相互作用解析

    光野恵理子, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    キャピラリー電気泳動シンポジウム講演要旨集   40th (CD-ROM)   2020

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  • 多段階部分的注入アフィニティーキャピラリー電気泳動法による物質間相互作用解析

    光野恵理子, 久本秀明, 遠藤達郎, 末吉健志, 末吉健志

    日本分析化学会年会講演要旨集(Web)   69th   2020

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  • Fabrication and characterization of YAG:Ce<sup>3+</sup> phosphor powder contained polymer-based photonic crystal for optical sensor application

    植田渉太郎, 小林奈緒, 川崎大輝, 山田大空, 末吉健志, 末吉健志, 久本秀明, 遠藤達郎, 遠藤達郎

    応用物理学会春季学術講演会講演予稿集(CD-ROM)   67th   2020

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  • ミクロスケール電気泳動フィルタリングに基づく核酸アプタマー高効率選抜デバイスの開発

    飛田安梨沙, 高尾隼空, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    化学とマイクロ・ナノシステム学会研究会講演要旨集   42nd (Web)   2020

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  • ミクロスケール電気泳動フィルタリングによるアプタマー選抜法の開発

    飛田安梨沙, 上野楓, 高尾隼空, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    キャピラリー電気泳動シンポジウム講演要旨集   40th (CD-ROM)   2020

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  • ナノ流路内電気二重層厚さ制御に基づくエクソソーム分級

    藤原聡子, 森川響二朗, 遠藤達郎, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    キャピラリー電気泳動シンポジウム講演要旨集   39th   2019

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  • キャピラリー分子ふるい電気泳動による低分子認識アプタマーの高効率選抜法

    和田将英, 遠藤達郎, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    キャピラリー電気泳動シンポジウム講演要旨集   39th   2019

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  • Inkjet Printingを用いた試薬固定に基づく1stepビオチン測定マイクロデバイスの開発

    河相優子, 角田正也, 末吉健志, 遠藤達郎, 久本秀明

    化学とマイクロ・ナノシステム学会研究会講演要旨集   39th   2019

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  • Inkjet Printingを用いた1step競合型イムノアッセイマイクロデバイスの開発

    河相優子, 角田正也, 末吉健志, 遠藤達郎, 久本秀明

    日本分析化学会年会講演要旨集(Web)   68th   2019

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  • 部分的注入アフィニティキャピラリー電気泳動に基づく核酸標的薬剤スクリーニング

    末吉健志, 末吉健志, 光野恵理子, 西野智哉, 遠藤達郎, 遠藤達郎, 久本秀明

    キャピラリー電気泳動シンポジウム講演要旨集   39th   2019

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  • 電気泳動フィルタリングによる酵素濃縮・生成物分離を利用した酵素反応初速度測定

    高尾隼空, 遠藤達郎, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    キャピラリー電気泳動シンポジウム講演要旨集   39th   2019

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Presentations

  • Microscale Electrophoretic Separation for Aptamer Selection Invited International conference

    Kenji Sueyoshi

    RSC Tokyo International Conference 2022  2022.12  The Royal Society of Chemistry, JAPAN Analytical Instruments Manufacturers’ Association

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    Presentation type:Oral presentation (invited, special)  

    Venue:Kawasaki Life Science and Environment Research Center (LiSE)  

  • さきがけ「生体における微粒子の機能と制御」第5回成果報告会〜体内外微粒子の挙動から見えてくる世界〜 エクソソームの由来判別・生体内動態解析のための粒子径分級およびアプタマータグ選抜・解析法の開発

    末吉 健志

    日本生化学会大会プログラム・講演要旨集  2022.11  (公社)日本生化学会

  • ミクロスケール電気泳動フィルタリングデバイスを用いた高効率アプタマー選抜 Domestic conference

    高尾隼空,上野楓,飛田安梨沙,遠藤達郎,久本秀明,末吉健志

    化学とマイクロ・ナノシステム学会 第46回研究会  2022.11  化学とマイクロ・ナノシステム学会

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    Presentation type:Poster presentation  

    Venue:アスティとくしま  

  • さきがけ「生体における微粒子の機能と制御」第5回成果報告会~体内外微粒子の挙動から見えてくる世界~ エクソソームの由来判別・生体内動態解析のための粒子径分級およびアプタマータグ選抜・解析法の開発

    末吉 健志

    日本生化学会大会プログラム・講演要旨集  2022.11  (公社)日本生化学会

  • 構造変化を利用した低分子標的アプタマーのCE選抜 Domestic conference

    和田将英,遠藤達郎,久本秀明,末吉健志

    第42回キャピラリー電気泳動シンポジウム  2022.10 

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    Presentation type:Oral presentation (general)  

  • ミクロスケール電気泳動フィルタリングデバイスを用いたアプタマー選抜法 Domestic conference

    末吉健志,高尾隼空,飛田安梨沙,上野楓,遠藤達郎,久本秀明

    第42回キャピラリー電気泳動シンポジウム  2022.10  日本分析化学会 電気泳動分析研究懇談会

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    Presentation type:Poster presentation  

    Venue:鶴岡市先端研究産業支援センター  

  • ミクロスケール電気泳動フィルタリングによる異種標的分子混合試料に対するアプタマー同時選抜 Domestic conference

    上野楓,遠藤達郎,久本秀明,末吉健志

    第42回キャピラリー電気泳動シンポジウム  2022.10  日本分析化学会 電気泳動分析研究懇談会

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    Presentation type:Poster presentation  

    Venue:鶴岡市先端研究産業支援センター  

  • Aptamer selection using a microscale electrophoretic filtration device Invited International conference

    Kenji Sueyoshi

    CE in the Biotechnology & Pharmaceutical Industries: Symposium on the Practical Applications for the Analysis of Proteins, Nucleotides & Small Molecules (CE Pharm 2022)  2022.09  California Separation Science Society

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    Presentation type:Oral presentation (invited, special)  

    Venue:Hilton Portland Downtown in Portland, OR USA  

  • ミクロスケール電気泳動フィルタリングに基づく簡便なアプタマー選抜 Domestic conference

    高尾隼空,遠藤達郎,久本秀明,末吉健志

    日本分析化学会第71年会  2022.09  日本分析化学会

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    Presentation type:Oral presentation (general)  

    Venue:岡山大学  

  • キャピラリー分子ふるい電気泳動法による低分子標的・構造誘起型アプタマー選抜 Domestic conference

    和田将英,遠藤達郎,久本秀明,末吉健志

    日本分析化学会第71年会  2022.09 

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    Presentation type:Oral presentation (general)  

    Venue:岡山大学  

  • 電気泳動フィルタリングデバイスを用いたエクソソームアプタマー選抜・解析 Domestic conference

    末吉健志,飛田安梨沙,上野楓,高尾隼空,遠藤達郎,久本秀明

    日本分析化学会第71年会  2022.09  日本分析化学会

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    Venue:岡山大学  

  • キャピラリー分子ふるい電気泳動による低分子標的構造誘起型アプタマー選抜 Domestic conference

    和田将英,遠藤達郎,久本秀明,末吉健志

    第29回クロマトグラフィーシンポジウム  2022.06  クロマトグラフィー科学会

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    Venue:石垣市民会館  

  • 部分的注入-非平衡アフィニティキャピラリー電気泳動に基づく核酸-低分子間相互作用解析 Domestic conference

    末吉健志,光野恵理子,遠藤達郎,久本秀明

    第29回クロマトグラフィーシンポジウム  2022.06  クロマトグラフィー科学会

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    Venue:石垣市民会館  

  • ミクロスケール電気泳動フィルタリングデバイスを用いた1ラウンドアプタマー選抜 Domestic conference

    末吉健志,高尾隼空,飛田安梨沙,上野楓,遠藤達郎,久本秀明

    化学とマイクロ・ナノシステム学会 第45回研究会  2022.05  化学とマイクロ・ナノシステム学会

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    Venue:中央大学  

  • マイクロ・ナノ流路内電気二重層厚さ調節に基づくナノ粒子分級用デバイスの開発 Domestic conference

    阿部友哉,藤原聡子,森川響二朗,遠藤達郎,久本秀明,末吉健志

    化学とマイクロ・ナノシステム学会 第45回研究会  2022.05  化学とマイクロ・ナノシステム学会

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    Venue:中央大学  

  • 核酸アプタマー選抜におけるキャピラリー分子ふるい電気泳動に基づく構造特性分離 Domestic conference

    末吉健志,和田将英,遠藤達郎,久本秀明

    日本分析化学会 第82回分析化学討論会  2022.05  日本分析化学会

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    Venue:茨城大学  

  • ミクロスケール電気泳動フィルタリングによるエクソソームアプタマー選抜 Domestic conference

    飛田安梨沙,上野楓,高尾隼空,遠藤達郎,久本秀明,末吉健志

    第41回キャピラリー電気泳動シンポジウム  2021.12  日本分析化学会 電気泳動分析研究懇談会

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  • 部分的注入アフィニティキャピラリー電気泳動による四重鎖核酸-低分子間相互作用解析 Domestic conference

    光野恵理子,遠藤達郎,久本秀明,末吉健志

    第41回キャピラリー電気泳動シンポジウム  2021.12  日本分析化学会 電気泳動分析研究懇談会

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  • キャピラリー分子ふるい電気泳動によるランダム配列DNAの構造安定性に基づく分離 Domestic conference

    和田将英,遠藤達郎,久本秀明,末吉健志

    第41回キャピラリー電気泳動シンポジウム  2021.12  日本分析化学会 電気泳動分析研究懇談会

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  • Aptamer Selection Based on Microscale Electrophoretic Filtration Invited International conference

    Kenji Sueyoshi

    Pacifichem 2021  2021.12  日本化学会 他

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  • Development of highly sensitive and selective plasticized PVC membrane optical sensor based on silver-responsive ionic liquid based-dye International conference

    Shuto Oka, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Royal Society of Chemistry Tokyo International Conference 2021  2021.12 

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  • Highly-sensitive cation sensing optodes based on FRET-enhanced fluorescence using fluorescent dyed plasticizer

    Kosuke Nishiumi, Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Royal Society of Chemistry Tokyo International Conference 2021  2021.12 

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  • Quantum Dots / TiO2 Hybrid Photonic Crystal: Fabrication and Characterization for Optical Biosensor Application in Visible Region International conference

    Shotaro Ueda, Daiki Kawasaki, Hirotaka Yamada, Ryosuke Nishitsuji, Kenji Sueyoshi, Hideaki Hisamoto, and Tatsuro Endo

    Royal Society of Chemistry Tokyo International Conference 2021  2021.12 

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  • ポリマー製フォトニック結晶シートを用いたスマートフォンによるSERS-CoV-2スパイクタンパク質の高感度検出 Domestic conference

    川崎大輝,山田大空,末吉健志,久本秀明,遠藤達郎

    第44回研究会  2021.11  化学とマイクロ・ナノシステム学会

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  • イオン選択性可塑化PVC複合型プラズモニックイオンセンサの開発 Domestic conference

    遠藤達郎,井上千種,川崎大輝,山田大空,末吉健志,久本秀明

    第38回「センサ・マイクロマシンと応用システム」シンポジウム  2021.11 

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  • ミクロスケール電気泳動フィルタリングデバイスを用いた核酸アプタマー選抜 Domestic conference

    高尾隼空,飛田安梨沙,上野楓,遠藤達郎,久本秀明,末吉健志

    第44回研究会  2021.11  化学とマイクロ・ナノシステム学会

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  • 高感度イオン検出を志向した色素液体ナノエマルションの開発とマイクロ分析デバイスへの応用 Domestic conference

    牧佳穂,末吉健志,遠藤達郎,久本秀明

    第44回研究会  2021.11  化学とマイクロ・ナノシステム学会

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  • ヒドロゲル部分充填キャピラリーデバイスを用いた高効率アプタマー電気泳動選抜法の開発 Domestic conference

    末吉健志,高尾隼空,飛田安梨沙,上野楓,遠藤達郎,久本秀明

    第32回クロマトグラフィー科学会議  2021.11  クロマトグラフィー科学会

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  • キャピラリー分子ふるい電気泳動に基づく低分子標的構造誘起型アプタマー選抜法 Domestic conference

    和田将英,遠藤達郎,久本秀明,末吉健志

    第32回クロマトグラフィー科学会議  2021.11  クロマトグラフィー科学会

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  • ミクロスケール電気泳動フィルタリングに基づくアプタマー選抜法の基礎評価・高効率化 Domestic conference

    上野楓,高尾隼空,飛田安梨沙,遠藤達郎,久本秀明,末吉健志

    第11回CSJ化学フェスタ2021  2021.10  日本化学会

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  • IONIC LIQUID-BASED DYE (IL-DYE) NANOEMULSION (NE) AS A HIGH-SENSITIVITY ION SENSING COMPONENT OF MICRO ANALYTICAL DEVICES International conference

    Kaho Maki, Kenji Sueyoshi, Tatsuro Endo, and Hideaki Hisamoto

    The 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences  2021.10 

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  • 銀イオン応答性色素液体の開発と高感度・高選択的オプティカルセンサーへの応用 Domestic conference

    岡嵩人,末吉健志,遠藤達郎,久本秀明

    第70年会  2021.09  日本分析化学会

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  • FRET型蛍光色素液体ナノエマルションの応答機構の基礎検討 Domestic conference

    小泉有右,水田巽,末吉健志,遠藤達郎,久本秀明

    第70年会  2021.09  日本分析化学会

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  • キャピラリー分子ふるい電気泳動に基づく低分子標的構造誘起型アプタマー選抜法 Domestic conference

    末吉健志,和田将英,遠藤達郎,久本秀明

    第70年会  2021.09  日本分析化学会

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  • 固定化不要な標的分子捕捉および高効率なDNA結合を利用したアプタマーの電気泳動選抜法 Domestic conference

    高尾隼空,飛田安梨沙,上野楓,久本秀明,遠藤達郎,末吉健志

    第70年会  2021.09  日本分析化学会

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  • 特発性肺ヘモジデローシスの一例

    比嘉 大地, 末吉 健志, 高良 博明

    断層映像研究会雑誌  2021.09  断層映像研究会

  • 低分子化合物認識素子としての新規アプタマー溶液系選抜・活用戦略 Domestic conference

    末吉健志

    イノベーション・ジャパン2021~大学見本市Online  2021.08  科学技術振興機構

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  • ミクロスケール電気泳動フィルタリングによるアプタマー選抜法の開発 Domestic conference

    飛田安梨沙,上野楓,高尾隼空,遠藤達郎,久本秀明,末吉健志

    第6回年会  2021.06  日本核酸医薬学会

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  • アプタマー選抜のための安定構造形成型・非形成型DNAライブラリーの調製 Domestic conference

    和田将英,遠藤達郎,久本秀明,末吉健志

    第6回年会  2021.06  日本核酸医薬学会

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  • キャピラリー分子ふるい電気泳動に基づく低分子標的アプタマー選抜のための構造形成型/非形成型核酸ライブラリの構築 Domestic conference

    末吉健志,和田将英,遠藤達郎,久本秀明

    第28回クロマトグラフィーシンポジウム  2021.06  クロマトグラフィー科学会

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  • 核酸アプタマー選抜・解析に基づく生体由来夾雑系試料の評価法開発 Invited Domestic conference

    末吉健志

    第60回日本生体医工学会大会・第36回日本生体磁気学会大会  2021.06  日本生体医工学会・日本生体磁気学会

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  • フォトニック結晶を用いたDNAメチル化の光検出 Domestic conference

    中島悠佑,小林奈緒,植田渉太郎,川崎大輝,末吉健志,久本秀明,遠藤達郎

    化学とマイクロ・ナノシステム学会 第43回研究会  2021.05  化学とマイクロ・ナノシステム学会

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  • ミクロスケール電気泳動フィルタリングによる高効率アプタマー選抜法の開発 Domestic conference

    上野楓,高尾隼空,飛田安梨沙,遠藤達郎,久本秀明,末吉健志

    第81回分析化学討論会  2021.05  日本分析化学会

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  • FRET機構を利用した超高感度カチオンセンシング用蛍光色素液体薄膜の開発 Domestic conference

    西海豪祐,水田巽,末吉健志,遠藤達郎,久本秀明

    第81回分析化学討論会  2021.05  日本分析化学会

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  • 多段階部分的注入アフィニティキャピラリー電気泳動に基づく核酸-低分子化合物間相互作用解析 Domestic conference

    光野恵理子,遠藤達郎,久本秀明,末吉健志

    第81回分析化学討論会  2021.05  日本分析化学会

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  • 低分子標的アプタマー選抜のためのキャピラリー分子ふるい電気泳動 Domestic conference

    和田将英,遠藤達郎,久本秀明,末吉健志

    第81回分析化学討論会  2021.05  日本分析化学会

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  • An enzyme-responsive fluorescent ionic liquid-based nanosensor toward highly sensitive biosensing International conference

    R. Oishi, T. Mizuta, K. Sueyoshi, T. Endo, H. Hisamoto

    RSC Tokyo International Conference 2020  2020.12 

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  • Size sorting of exosomes by tuning the thicknesses of the electric double layers in nanochannels International conference

    S. Fujiwara, K. Morikawa, T. Endo, H. Hisamoto, K. Sueyoshi

    RSC Tokyo International Conference 2020  2020.12 

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  • Preparation of ionic liquid-based dye (IL-Dye) nanoemulsion (NE) for high-sensitivity ion sensing International conference

    K. Maki, R. Oishi, T. Mizuta, K. Sueyoshi, T. Endo, H. Hisamoto

    RSC Tokyo International Conference 2020  2020.12 

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  • キャピラリー分子ふるい電気泳動による低分子標的核酸アプタマー選抜法 Domestic conference

    和田将英,遠藤達郎,久本秀明,末吉健志

    40周年記念キャピラリー電気泳動シンポジウム  2020.11 

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  • 電気泳動フィルタリングによる標的分子捕捉と DNA 結合・解離を利用した新規アプタマー選抜法の開発 Domestic conference

    高尾隼空,飛田安梨沙,上野楓,遠藤達郎,久本秀明,末吉健志

    40周年記念キャピラリー電気泳動シンポジウム  2020.11 

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  • ミクロスケール電気泳動フィルタリングによるアプタマー選抜法の開発 Domestic conference

    飛田安梨沙,上野楓,高尾隼空,遠藤達郎,久本秀明,末吉健志

    40周年記念キャピラリー電気泳動シンポジウム  2020.11 

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  • 多段階部分的注入アフィニティーキャピラリー電気泳動法による疾患関連核酸-低分子化合物間相互作用解析 Domestic conference

    光野恵理子, 遠藤達郎, 久本秀明, 末吉健志

    40周年記念キャピラリー電気泳動シンポジウム  2020.11 

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  • キャピラリー電気泳動をこれから始める方へ Domestic conference

    末吉健志

    40周年記念キャピラリー電気泳動シンポジウム  2020.11 

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  • ミクロスケール電気泳動フィルタリングに基づく核酸アプタマー高効率選抜デバイスの開発 Domestic conference

    飛田安梨沙,高尾隼空,遠藤達郎,久本秀明,末吉健志

    化学とマイクロ・ナノシステム学会 第42回研究会  2020.10 

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  • 光学バイオセンサ応用を志向したYAG:Ce3+粒子含有フォトニック結晶の作製と光学特性評価 Domestic conference

    植田渉太郎,小林奈緒,川崎大輝,山田大空,末吉健志,久本秀明,遠藤達郎

    「センサ・マイクロマシンと応用システム」シンポジウム  2020.10 

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  • ミクロスケール電気泳動フィルタリングに基づくエクソソームアプタマー選抜の基礎検討 Domestic conference

    飛田安梨沙,永井伶奈,高尾隼空,遠藤達郎,久本秀明,末吉健志

    第10回CSJ化学フェスタ2020  2020.10 

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  • 液状イオノフォアに基づく高感度・高選択的アンモニウムイオンセンシングを志向した可塑化PVC膜センサーの基礎検討 Domestic conference

    岡嵩人,水田巽,末吉健志,遠藤達郎,久本秀明

    第10回CSJ化学フェスタ2020  2020.10 

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  • キャピラリー分子ふるい電気泳動に基づく低分子標的アプタマー選抜法 Domestic conference

    和田将英,遠藤達郎,久本秀明,末吉健志

    日本分析化学会 第69年会  2020.09 

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  • 多段階部分的注入アフィニティーキャピラリー電気泳動法による物質間相互作用解析 Domestic conference

    光野恵理子,遠藤達郎,久本秀明,末吉健志

    日本分析化学会 第69年会  2020.09 

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  • 蛍光性色素液体を用いたFRET検出型PVC膜オプトードの開発と超高感度イオンセンシングへの応用 Domestic conference

    水田巽,末吉健志,遠藤達郎,久本秀明

    日本分析化学会 第69年会  2020.09 

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  • 高感度イオンセンシングを志向した色素液体ナノエマルションの創製 Domestic conference

    牧佳穂,大石綾太郎,水田巽,末吉健志,遠藤達郎,久本秀明

    日本分析化学会 第69年会  2020.09 

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  • プラズモニック結晶を用いた非染色PCR産物検出デバイスの開発 Domestic conference

    古川渢大,井上千種,川崎大輝,山田大空,末吉健志,久本秀明,遠藤達郎

    日本分析化学会 第69年会  2020.09 

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  • 高精度な細胞外微粒子分級用マイクロ・ナノ流体デバイスの開発 Domestic conference

    藤原聡子,金子紗也,森川響二朗,遠藤達郎,久本秀明,末吉健志

    日本分析化学会 第69年会  2020.09 

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  • 核酸アプタマー高効率選抜のためのミクロスケール電気泳動フィルタリングデバイス Domestic conference

    末吉健志,永井伶奈,遠藤達郎,久本秀明

    日本分析化学会 第69年会  2020.09 

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  • 高精度な細胞外微粒子分級用マイクロ・ナノ流体デバイスの開発

    藤原 聡子, 金子 紗也, 森川 響二朗, 遠藤 達郎, 久本 秀明, 末吉 健志

    日本分析化学会講演要旨集  2020.09  (公社)日本分析化学会

  • 多段階部分的注入アフィニティーキャピラリー電気泳動法による物質間相互作用解析

    光野 恵理子, 久本 秀明, 遠藤 達郎, 末吉 健志

    日本分析化学会講演要旨集  2020.09  (公社)日本分析化学会

  • プラズモニック結晶を用いた非染色PCR産物検出デバイスの開発

    古川 ふう大, 井上 千種, 川崎 大輝, 山田 大空, 末吉 健志, 久本 秀明, 遠藤 達郎

    日本分析化学会講演要旨集  2020.09  (公社)日本分析化学会

  • プラズモニック結晶を用いた非染色PCR産物検出デバイスの開発

    古川 ふう大, 井上 千種, 川崎 大輝, 山田 大空, 末吉 健志, 久本 秀明, 遠藤 達郎

    日本分析化学会講演要旨集  2020.09  (公社)日本分析化学会

  • 高精度な細胞外微粒子分級用マイクロ・ナノ流体デバイスの開発

    藤原 聡子, 金子 紗也, 森川 響二朗, 遠藤 達郎, 久本 秀明, 末吉 健志

    日本分析化学会講演要旨集  2020.09  (公社)日本分析化学会

  • 多段階部分的注入アフィニティーキャピラリー電気泳動法による物質間相互作用解析

    光野 恵理子, 久本 秀明, 遠藤 達郎, 末吉 健志

    日本分析化学会講演要旨集  2020.09  (公社)日本分析化学会

  • キャピラリー分子ふるい電気泳動を利用した低分子標的アプタマー選抜法 Domestic conference

    和田将英,遠藤達郎,久本秀明,末吉健志

    日本分析化学会 第80回分析化学討論会  2020.05 

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  • 多段階部分的注入キャピラリー電気泳動法による核酸-低分子化合物間相互作用解析 Domestic conference

    光野恵理子,遠藤達郎,久本秀明,末吉健志

    日本分析化学会 第80回分析化学討論会  2020.05 

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  • 蛍光性色素液体を用いた超高感度FRET検出型PVC膜オプトードの開発 Domestic conference

    水田巽,末吉健志,遠藤達郎,久本秀明

    日本分析化学会 第80回分析化学討論会  2020.05 

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  • 色素液体ナノエマルションの創製と高感度イオンセンシングの基礎検討 Domestic conference

    牧佳穂,大石綾太郎,水田巽,末吉健志,遠藤達郎,久本秀明

    日本分析化学会 第80回分析化学討論会  2020.05 

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  • マイクロ・ナノ流路デバイス内電気二重層制御に基づくエクソソームの粒子径分級 Domestic conference

    末吉健志

    日本分析化学会 第80回分析化学討論会  2020.05 

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  • 液状イオノフォアと固体色素に基づく高感度アンモニウムイオンセンシング液体開発の基礎検討 Domestic conference

    岡嵩人,水田巽,末吉健志,遠藤達郎,久本秀明

    日本分析化学会 第80回分析化学討論会  2020.05 

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  • Aptamer Selection based on Microscale Electrophoresis International conference

    K. Sueyoshi

    The Pittsburgh Conference & Exposition 2020 (PITTCON 2020)  2020.03 

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  • 部分的注入アフィニティキャピラリー電気泳動に基づく核酸標的薬剤スクリーニング法の開発 Domestic conference

    末吉健志,光野恵理子,西野智哉,遠藤達郎,久本秀明

    第30回クロマトグラフィー科学会議  2019.12 

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  • Capillary sieving electrophoresis for selection of aptamers targeting small molecules International conference

    M. Wada, T. Endo, H. Hisamoto, K. Sueyoshi

    49th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC2019 Kyoto)  2019.12 

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  • Nanoparticle Classification Using A “Tunable Nanogate” Device International conference

    K. Sueyoshi, S. Fujiwara, T. Endo, H. Hisamoto

    49th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC2019 Kyoto)  2019.12 

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  • パネルディスカッション ~CHEMINASにおけるクリエイティビティとこれから~ Domestic conference

    田澤英克,末吉健志,太田禎生,福山真央,川田治良,小松雄士,南木創

    化学とマイクロ・ナノシステム学会 第40回研究会  2019.11 

  • キャピラリー分子ふるい電気泳動による低分子認識アプタマーの高効率選抜法の開発 Domestic conference

    和田将英,遠藤達郎,久本秀明,末吉健志

    第39回キャピラリー電気泳動シンポジウム  2019.11 

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  • ナノ流路内電気二重層厚さ制御に基づくエクソソーム分級 Domestic conference

    藤原聡子,森川響二郎,遠藤達郎,久本秀明,末吉健志

    第39回キャピラリー電気泳動シンポジウム  2019.11 

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  • 電気泳動フィルタリングによる酵素濃縮・生成物分離を利用した酵素反応初速度測定 Domestic conference

    高尾隼空,遠藤達郎,久本秀明,末吉健志

    第39回キャピラリー電気泳動シンポジウム  2019.11 

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  • 部分注入アフィニティキャピラリー電気泳動に基づく核酸標的薬剤スクリーニング Domestic conference

    末吉健志,光野恵理子,西野智哉,遠藤達郎,久本秀明

    第39回キャピラリー電気泳動シンポジウム  2019.11 

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  • 沖縄型神経原性筋萎縮症における脊髄萎縮 特にALSとの比較において

    藤崎 なつみ, 諏訪園 秀吾, 末吉 健志, 中地 亮, 城戸 美和子, 藤原 善寿, 渡嘉敷 崇, 妹尾 洋, 立田 直久, 赤嶺 博行

    臨床神経学  2019.11  (一社)日本神経学会

  • "CALCIUM-RESPONSIVE IONIC LIQUID" FOR NAKED EYE-BASED MULTIPLEXED ION DETECTION ON A PDMS MICROCHANNEL ARRAY DEVICE International conference

    T. Mizuta, Y. Niwa, K. Sueyoshi, T. Endo, H. Hisamoto

    The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2019)  2019.10 

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  • INKJET-PRINTED SINGLE-STEP COMPETITIVE IMMUNOASSAY MICRODEVICE FOR THE DETECTION OF CRP International conference

    Y. Kawai, M. Kakuta, K. Sueyoshi, T. Endo, H. Hisamoto

    The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2019)  2019.10 

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  • AN "ENZYME-RESPONSIVE IONIC LIQUID" TOWARD CAPILLARY ARRAY-BASED IMMUNOASSAY MICRODEVICES International conference

    R. Oishi, T. Mizuta, K. Sueyoshi, T. Endo, H. Hisamoto

    The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2019)  2019.10 

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  • "TUNABLE NANOGATE" DEVICE FOR SIZE-SORTING OF NANOPARTICLES International conference

    S. Fujiwara, T. Endo, H. Hisamoto, K. Sueyoshi

    The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2019)  2019.10 

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  • MICROFLUIDIC DEVICE FOR DIRECT MEASUREMENT OF INITIAL RATE OF ENZYME REACTION BY ELECTROPHORETIC FILTRATION International conference

    J. Takao, T. Endo, H. Hisamoto, K. Sueyoshi

    The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2019)  2019.10 

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  • 潜行性に経過し、大脳皮質基底核症候群様の症状を呈したCADASILの一例

    遠藤 一博, 渡嘉敷 崇, 末吉 健志

    Dementia Japan  2019.10  (一社)日本認知症学会

  • 広域プラズモン増強電場発生を示すMetal-Insulator-Metalドットアレイの開発 Domestic conference

    山田大空,川﨑大輝,井上千種,前野権一,末吉健志,久本秀明,遠藤達郎

    第80回応用物理学会秋季学術講演会  2019.09 

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  • キャピラリー分子ふるい電気泳動に基づく低分子標的アプタマー選抜法の開発 Domestic conference

    和田将英,遠藤達郎,久本秀明,末吉健志

    日本分析化学会第68年会  2019.09 

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  • モールド形状制御によるナノインプリント製プラズモニック結晶のセンサ性能制御 Domestic conference

    山田大空,志水友哉,小林奈緒,末吉健志,久本秀明,遠藤達郎

    日本分析化学会第68年会  2019.09 

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  • 蛍光性色素液体に基づく可塑化PVC膜センサーの開発:色素構造と蛍光特性の基礎検討 Domestic conference

    水田巽,末吉健志,遠藤達郎,久本秀明

    日本分析化学会第68年会  2019.09 

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  • 電気泳動フィルタリングに基づく酵素反応初速度直接測定法の開発 Domestic conference

    高尾隼空,遠藤達郎,久本秀明,末吉健志

    日本分析化学会第68年会  2019.09 

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  • Inkjet Printingを用いた1 step競合型イムノアッセイマイクロデバイスの開発 Domestic conference

    河相優子,角田正也,末吉健志,遠藤達郎,久本秀明

    日本分析化学会第68年会  2019.09 

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  • 簡便・迅速・高感度な1stepELISAへの応用を指向した酵素応答性疎水性色素液体の開発 Domestic conference

    大石綾太郎,水田巽,末吉健志,遠藤達郎,久本秀明

    日本分析化学会第68年会  2019.09 

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  • 電気泳動フィルタリングに基づく酵素反応初速度直接測定法の開発

    高尾 隼空, 遠藤 達郎, 久本 秀明, 末吉 健志

    日本分析化学会講演要旨集  2019.08  (公社)日本分析化学会

  • 簡便・迅速・高感度な1stepELISAへの応用を指向した酵素応答性疎水性色素液体の開発

    大石 綾太郎, 水田 巽, 末吉 健志, 遠藤 達郎, 久本 秀明

    日本分析化学会講演要旨集  2019.08  (公社)日本分析化学会

  • キャピラリー分子ふるい電気泳動に基づく低分子標的アプタマー選抜法の開発

    和田 将英, 遠藤 達郎, 久本 秀明, 末吉 健志

    日本分析化学会講演要旨集  2019.08  (公社)日本分析化学会

  • Inkjet Printingを用いた1 step競合型イムノアッセイマイクロデバイスの開発

    河相 優子, 角田 正也, 末吉 健志, 遠藤 達郎, 久本 秀明

    日本分析化学会講演要旨集  2019.08  (公社)日本分析化学会

  • 電気泳動フィルタリングに基づく酵素反応初速度直接測定法の開発

    高尾 隼空, 遠藤 達郎, 久本 秀明, 末吉 健志

    日本分析化学会講演要旨集  2019.08  (公社)日本分析化学会

  • 簡便・迅速・高感度な1stepELISAへの応用を指向した酵素応答性疎水性色素液体の開発

    大石 綾太郎, 水田 巽, 末吉 健志, 遠藤 達郎, 久本 秀明

    日本分析化学会講演要旨集  2019.08  (公社)日本分析化学会

  • キャピラリー分子ふるい電気泳動に基づく低分子標的アプタマー選抜法の開発

    和田 将英, 遠藤 達郎, 久本 秀明, 末吉 健志

    日本分析化学会講演要旨集  2019.08  (公社)日本分析化学会

  • Inkjet Printingを用いた1 step競合型イムノアッセイマイクロデバイスの開発

    河相 優子, 角田 正也, 末吉 健志, 遠藤 達郎, 久本 秀明

    日本分析化学会講演要旨集  2019.08  (公社)日本分析化学会

  • キャピラリー分子ふるい電気泳動を利用した低分子認識アプタマーの高効率選抜法の開発 Domestic conference

    和田将英,遠藤達郎,久本秀明,末吉健志

    日本核酸医薬学会 第5回年会  2019.07 

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  • 腰椎椎間板ヘルニアのタイプの診断におけるMRIと椎間板造影CTの比較

    渋谷 まり子, 平良 豊, 比嘉 康敏, 末吉 健志

    日本ペインクリニック学会誌  2019.06  (一社)日本ペインクリニック学会

  • 高選択的イオン応答性色素液体の開発とマルチイオンセンシングデバイスへの応用 Domestic conference

    水田巽,丹羽祐介,末吉健志,遠藤達郎,久本秀明

    化学とマイクロ・ナノシステム学会 第39回研究会  2019.05 

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  • ナノ空間内電気二重層厚さ制御に基づくナノ粒子分級デバイスの基礎検討 Domestic conference

    藤原聡子,遠藤達郎,久本秀明,末吉健志

    化学とマイクロ・ナノシステム学会 第39回研究会  2019.05 

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  • 高感度蛍光増強型バイオセンシングを志向したTiO2 / ハイドロゲルハイブリッドフォトニック結晶の作製 Domestic conference

    小林奈緒,志水友哉,川﨑大輝,山田大空,久本秀明,末吉健志,遠藤達郎

    化学とマイクロ・ナノシステム学会 第39回研究会  2019.05 

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  • Inkjet Printingを用いた試薬固定に基づく1 stepビオチン測定マイクロデバイスの開発 Domestic conference

    河相優子,角田正也,末吉健志,遠藤達郎,久本秀明

    化学とマイクロ・ナノシステム学会 第39回研究会  2019.05 

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  • 低分子認識アプタマーのキャピラリー分子ふるい電気泳動に基づく高効率選抜法の開発 Domestic conference

    和田将英,西野智哉,遠藤達郎,久本秀明,末吉健志

    日本分析化学会 第79回分析化学討論会  2019.05 

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  • ミクロスケール電気泳動に基づく濃縮・混合・分離を利用した酵素反応初速度測定法の開発 Domestic conference

    高尾隼空,遠藤達郎,久本秀明,末吉健志

    日本分析化学会 第79回分析化学討論会  2019.05 

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  • キャピラリー電気泳動を用いた低分子-核酸相互作用ハイスループットスクリーニング Domestic conference

    末吉健志,西野智哉,遠藤達郎,久本秀明

    日本分析化学会 第79回分析化学討論会  2019.05 

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Collaborative research (seeds) keywords

  • キャピラリー電気泳動

  • マイクロチップ電気泳動

  • マイクロ化学分析チップ

Outline of collaborative research (seeds)

  • Microscale Bioassays Based on Electrophoresis

  • Microchip Electrophoresis

  • Capillary Electrophoresis

Charge of on-campus class subject

  • Advanced Applied Chemistry III

    2021    

  • Laboratory Exercises for Applied Chemistry II

    2021   Practical Training  

  • Laboratory Exercises for Applied Chemistry I

    2021   Practical Training  

  • Exercises in Physical Chemistry IA

    2021    

  • Introduction to Physical Chemistry

    2021    

  • Laboratory Exercises for Applied Chemistry V

    2021   Practical Training  

  • Laboratory Exercises for Applied Chemistry III

    2021   Practical Training  

  • Instrumental Methods of Analysis

    2021    

  • Exercises in Physical Chemistry IIA

    2021    

  • Laboratory Exercises for Applied Chemistry IV

    2021   Practical Training  

  • Advanced Analytical Chemistry in Technology

    2021    

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Faculty development activities

  • 2021

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    応用化学実験I 実験テキスト 応用化学課程 全教員

  • 2021

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    応用化学実験II 実験テキスト 応用化学課程 全教員

  • 2021

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    応用化学実験III 実験テキスト 応用化学課程 全教員

  • 2021

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    応用化学実験IV・V 実験テキスト 応用化学課程 全教員

  • 2021

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    Exercises in Physical Chemistry IIA 堀内 悠、末吉 健志、亀川 孝、椎木 弘、樋口 栄次、作田 敦

  • 2020

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    物理化学演習IA オンラインテキスト 遠藤達郎、樋口栄次、知久昌信、亀川 孝、床波志保、 末吉健志

  • 2020

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    Exercises in Physical Chemistry IIA 竹内雅人、末吉健志、亀川 孝、椎木 弘、樋口栄次、作田 敦

  • 2020

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    応用化学実験IV・V 実験テキスト 応用化学課程 全教員

  • 2020

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    応用化学実験III 実験テキスト 応用化学課程 全教員

  • 2020

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    応用化学実験II 実験テキスト 応用化学課程 全教員

  • 2020

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    応用化学実験I 実験テキスト 応用化学課程 全教員

  • 2019

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    応用化学実験I 実験テキスト 応用化学課程 全教員

  • 2019

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    Exercises in Physical Chemistry IIA 竹内雅人,亀川孝,末吉健志,堀内悠

  • 2019

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    応用化学実験IV・V 実験テキスト 応用化学課程 全教員

  • 2019

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    応用化学実験III 実験テキスト 応用化学課程 全教員

  • 2019

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    応用化学実験II 実験テキスト 応用化学課程 全教員

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Academic Activities

  • Analyst

    2009 - Now

  • Analytical Sciences

    2009 - Now

  • Journal of Chromatography A

    2009 - Now

  • Journal of Pharmaceutical and Biomedical Analysis

    2009 - Now

  • CHROMATOGRAPHY

    2009 - Now

  • Sensors and Actuators: B. Chemical

    2009 - Now

  • Lab on a Chip

    2009 - Now

  • Journal of Separation Science

    2009 - Now

  • Electrophoresis

    2009 - Now

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Other

  • 研究職歴

    2018.01 - Now

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    大阪府立大学 大学院工学研究科 准教授

  • 研究職歴

    2013.01 - Now

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    大阪府立大学大学院工学研究科 テニュアトラック助教