Researcher Profiles - Matsubayashi Makoto

写真a

Matsubayashi Makoto

Organization

Graduate School of Veterinary Science Department of Veterinary Science Professor
School of Veterinary Science Department of Veterinary Science

Affiliation

Institute of Veterinary Science

Position

Graduate School of Veterinary Science Department of Veterinary Science

Professor 2022.04 - Now
School of Veterinary Science Department of Veterinary Science

Professor 2022.04 - Now

Degree

博士 (獣医学) （ Osaka Prefecture University ）

Research Areas

Life Science / Parasitology / 原虫学

Research subject summary

Apicomplexan 原虫の病態発現解析および弱毒化分子機構の解明
家畜や伴侶動物を対象とした寄生虫・原虫の分子疫学解析
消化管寄生原虫に対する新規薬剤の開発

Professional Memberships

日本獣医学会

1997.10 - Now Domestic
日独原虫病協会

2009.09 - Now Overseas
日本獣医師会

2000.10 - Now Domestic
日本獣医寄生虫学会

2009.09 - Now Domestic
鶏病研究会

2010.04 - Now Domestic
日本寄生虫学会

2000.10 - Now Domestic
日本食品衛生学会

2002.12 - 2010.03 Domestic
日本感染症学会

2006.12 - 2010.03 Domestic
日本家政学会

2002.11 - 2010.03 Domestic
日本動物園水族館協会

2006.04 - 2007.03 Domestic
日本寄生虫学会西日本支部会

2009.09 - 2010.03 Domestic

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Awards

令和５年全国農業共済協会家畜診療賞

髙橋和瑛, 松尾智英, 山岸潤也, 芝原友幸, 笹井和美, 松林誠

2023.12 一酪農家におけるクリプトスポリジウム症の清浄化への試み
日本獣医学会優秀発表賞

高野有人, 寺本勲, 畑生俊光, 城戸康年, 金子明, 笹井和美, 加藤宏光, 松林誠

2023.11 第166回日本獣医学会学術集会 Eimeria 属原虫のPCRのための鋳型DNA調整法の比較および超簡略化への試み
奨励賞.

高橋和瑛, 松尾智英, 山岸潤也, 芝原友幸, 笹井和美, 松林誠

2022.08 令和4年度獣医学術近畿地区学会・近畿地区連合獣医師大会 (日本産業動物獣医学会). 一酪農場におけるクリプトスポリジウム症の清浄化への試み.
クリタ水・環境科学研究優秀賞.

松林誠.

2021.08 公益財団法人クリタ水・環境科学振興財団. 水銀殺菌ランプに代わるUV-LEDの水中クリプトスポリジウム殺滅効果とin vivo 評価系の構築.
大阪府立大学学長懸賞.

松林誠.

2019.08 大阪府立大学.

Job Career (off-campus)

大阪公立大学大学院獣医学専攻専攻長/学科長

2023.04 - Now
インドネシア農業省国立獣医学研究所寄生虫学研究室共同研究員

2016.09 - Now

Papers

Distribution of Eimeria uekii and Eimeria raichoi in cage protection environments for the conservation of Japanese rock ptarmigans (Lagopus muta japonica) in the Japanese Alps

Makoto Matsubayashi, Atsushi Kobayashi, Minemitsu Kaneko, Moemi Kinoshita, Sayaka Tsuchida, Tomoyuki Shibahara, Masami Hasegawa, Hiroshi Nakamura, Kazumi Sasaia, Kazunari Ushida

Int J Parasitol Parasites Wildl 著書 2021

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Kind of work：Joint Work
Coproparasitological examinations and molecular determination of Eimeria species in Madura cattle reared on Madura Island, Indonesia Reviewed

Poedji Hastutiek, Nunuk Dyah Retno Lastuti, Lucia Tri Suwanti, Agus Sunarso, Endang Suprihati, Dyah Ayu Kurniawati, Makoto Matsubayashi

Parasitology International 2021

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Kind of work：Joint Work
Morphological and molecular identification of Eimeria tetartooimia oocysts from a Japanese green pheasant (Galliformes; Phasianidae; Phasianus versicolor) at a zoo in Japan Reviewed

Makoto Matsubayashi, Kazutoshi Takami, Moemi Kinoshita, Sayaka Tsuchida, Kazunari Ushida, Tomoyuki Shibahara, Kazumi Sasai

Parasitol Res 雑誌 2021

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Kind of work：Joint Work
Relationship between Eimeria tenella associated-early clinical signs and molecular changes in the intestinal barrier function

Pham HHS, Matsubayashi M, Tsuji N, Hatabu T

Research in Veterinary Science 雑誌 240 2021

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Kind of work：Joint Work
コクシジウムが関与した子牛の慢性壊死性腸炎. . Reviewed

杉江建之介, 小松徹也, 川本尚未, 橋本幸江, 松林誠, 芝原友幸.

家畜診療 2021

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Kind of work：Joint Work
Redescription, molecular characterisation and Wolbachia endosymbionts of Mansonella (Tupainema) dunni (Mullin & Orihel, 1972) (Spirurida: Onchocercidae) from the common treeshrew Tupaia glis Diard & Duvaucel (Mammalia: Scandentia) in Peninsular Malaysia

Mat Udin A.S.

Current Research in Parasitology and Vector-Borne Diseases 5 100154 2024.01

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DOI： 10.1016/j.crpvbd.2023.100154

PubMed
Improved molecular identification of Strongyloides myopotami in nutrias using fecal samples. Reviewed

Yuga MORI, Atsushi NAKA, Ryosuke KODA, Yuzuru ISHIZUKA, Atsushi HINENOYA, Tomoyuki SHIBAHARA, Kazumi SASAI, Makoto MATSUBAYASHI

The Journal of veterinary medical science 2024

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Authorship：Corresponding author Publishing type：Research paper (scientific journal)
Redescription, molecular characterisation and Wolbachia endosymbionts of Mansonella (Tupainema) dunni (Mullin & Orihel, 1972) (Spirurida: Onchocercidae) from the common treeshrew Tupaia glis Diard & Duvaucel (Mammalia: Scandentia) in Peninsular Malaysia. Reviewed

Current Research in Parasitology & Vector-Borne Diseases 2024

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Publishing type：Research paper (scientific journal)
Experimental evaluation of pathogenicity and acquired immunity of Eimeria species, E. uekii and E. raichoi, infecting Japanese rock ptarmigans in a subspecies of the birds

Matsubayashi M.

International Journal for Parasitology: Parasites and Wildlife 22 167 - 174 2023.12（ ISSN:22132244 ）

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DOI： 10.1016/j.ijppaw.2023.09.005

PubMed
Coprological Investigation of Gastrointestinal Parasites in Wild Birds Protected in Kochi Prefecture

INOUE Haruna, MORI Yuga, HATANAKA Noritoshi, SHIBAHARA Tomoyuki, SASAI Kazumi, MATSUBAYASHI Makoto

Japanese Journal of Zoo and Wildlife Medicine 28 ( 2 ) 103 - 106 2023.09（ ISSN:13426133 ）

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　Total 101 fecal samples were collected from the wild birds which were protected at Kochi Prefecture. These were examined by sugar floatation method. Consequently, 29.7％ of feces were parasitologically positive; 20.8% of Eimeria and/or Isospora type oocysts, and 8.9% of Capillarid or Ascarid spp. Transmitted stages of these parasites could survive in the environments for a while after being shedded in feces. These results suggested that in the past, these infected birds directly or indirectly contacted with feces contaminated with the parasites.

DOI： 10.5686/jjzwm.28.103
Experimental evaluation of pathogenicity and acquired immunity of Eimeria species, E. uekii and E. raichoi, infecting Japanese rock ptarmigans in a subspecies of the birds. Reviewed

Matsubayashi M, Kinoshita M, Tsuchida S, Kobayashi A, Tamura N, Shibahara T, Kido Y, Kaneko A, Sasai K, Ushida K

Int J Parasitol Parasites Wildl. 22 167 - 174. 2023.09

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Japanese rock ptarmigans (Lagopus muta japonica) are birds that inhabit only alpine regions of central Honshu Island, Japan, known as the Japanese Alps. The number of these birds has recently declined, and in situ and ex situ national conservation programs for Japanese rock ptarmigans have been initiated. The infections of Eimeria spp. as protozoan parasites of the phylum Apicomplexa, E. uekii and E. raichoi, were frequently reported in the birds. However, the virulence of these Eimeria parasites has not been determined. Here, we analyzed the pathogenicity of these Eimeria parasites using experimental infections of a subspecies model of Japanese rock ptarmigans, Svalbard rock ptarmigans (Lagopus mutus hyperboreus), and evaluated acquired protective immunity against challenge in birds tolerant of low-dose inoculation with Eimeria parasites. Following inoculation with two Eimeria parasites derived from Japanese rock ptarmigans (dose range of 4 × 104 to 4 × 102 for E. uekii and 1.7 × 104 to 4 × 101 for E. raichoi), oocysts were detected at 6-8 days post-inoculation (PI), and the maximum number of oocysts per gram of feces was observed 7-10 days PI and then gradually decreased. The mortality rate and reduction in weight gain of chicks increased following high-dose inoculation of oocysts with abnormal feces (soft and diarrhea). Developmental zoites were detected histopathologically in epithelial tissues and sometimes the lamina propria from the duodenum to the colon. Chicks that survived low-dose inoculation did not show clear clinical symptoms after challenge inoculation. Our results suggest that the pathological characteristics of Eimeria parasites infecting Japanese rock ptarmigans include abnormal feces and reduction in weight gain, resulting in mortality in cases of heavy infection due to high-dose inoculation. These findings provide helpful data for Japanese rock ptarmigan conservation efforts.

DOI： 10.1016/j.ijppaw.2023.09.005.

PubMed
Evaluation of the host specificity of Eimeria uekii and Eimeria raichoi for Japanese rock ptarmigans by oocyst transfer to taxonomically related birds

Matsubayashi M.

Parasitology Research 122 ( 8 ) 1795 - 1800 2023.08（ ISSN:09320113 ）

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DOI： 10.1007/s00436-023-07875-7

PubMed
一酪農家におけるクリプトスポリジウム症の清浄化への試み

高橋和瑛, 松尾智英, 山岸潤也, 芝原友幸, 笹井和美, 松林誠

家畜診療 70 ( 7 ) 407 - 416 2023.07（ ISSN:0287-0754 ）
RNA activation in ticks. Reviewed

Kofi Dadzie Kwofie, Emmanuel Pacia Hernandez, Anisuzzaman, Hayato Kawada, Yuki Koike, Sana Sasaki, Takahiro Inoue, Kei Jimbo, Fusako Mikami, Danielle Ladzekpo, Rika Umemiya-Shirafuji, Kayoko Yamaji, Tetsuya Tanaka, Makoto Matsubayashi, Md Abdul Alim, Samuel Kweku Dadzie, Shiroh Iwanaga, Naotoshi Tsuji, Takeshi Hatta

Scientific reports 13 ( 1 ) 9341 - 9341 2023.06

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

RNA activation (RNAa) is a burgeoning area of research in which double-stranded RNAs (dsRNAs) or small activating RNAs mediate the upregulation of specific genes by targeting the promoter sequence and/or AU-rich elements in the 3'- untranslated region (3'-UTR) of mRNA molecules. So far, studies on the phenomenon have been limited to mammals, plants, bacteria, Caenorhabditis elegans, and recently, Aedes aegypti. However, it is yet to be applied in other arthropods, including ticks, despite the ubiquitous presence of argonaute 2 protein, which is an indispensable requirement for the formation of RNA-induced transcriptional activation complex to enable a dsRNA-mediated gene activation. In this study, we demonstrated for the first time the possible presence of RNAa phenomenon in the tick vector, Haemaphysalis longicornis (Asian longhorned tick). We targeted the 3'-UTR of a novel endochitinase-like gene (HlemCHT) identified previously in H. longicornis eggs for dsRNA-mediated gene activation. Our results showed an increased gene expression in eggs of H. longicornis endochitinase-dsRNA-injected (dsHlemCHT) ticks on day-13 post-oviposition. Furthermore, we observed that eggs of dsHlemCHT ticks exhibited relatively early egg development and hatching, suggesting a dsRNA-mediated activation of the HlemCHT gene in the eggs. This is the first attempt to provide evidence of RNAa in ticks. Although further studies are required to elucidate the detailed mechanism by which RNAa occurs in ticks, the outcome of this study provides new opportunities for the use of RNAa as a gene overexpression tool in future studies on tick biology, to reduce the global burden of ticks and tick-borne diseases.

DOI： 10.1038/s41598-023-36523-4

PubMed
Occurrence, Histopathological Findings, and Molecular Identification of Pathogenic Eimeria Infections in Rabbits (Mammalia: Lagomorpha) in Japan. Reviewed

Shigeaki Takami, Tomoyuki Shibahara, Kazumi Sasai, Makoto Matsubayashi

Acta parasitologica 68 ( 2 ) 453 - 457 2023.04（ ISSN:12302821 ）

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Authorship：Corresponding author Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

PURPOSE: Eimeria spp. are commonly found among rabbits (Mammalia: Lagomorpha) worldwide. Among the 11 Eimeria species, several are highly virulent, including E. intestinalis and E. flavescens, which cause intestinal coccidiosis, and E. stiedae, which causes hepatic coccidiosis. Unlike other countries, the occurrence of Eimeria infections in rabbits in Japan remains unknown, except for one reported case of natural infection. METHODS: We surveyed Eimeria infections in clinically affected rabbits over the past approximately 10 years at Livestock Hygiene Centers in 42 prefectures. A total of 16 tissue samples (14 liver, 1 ileum, and 1 cecum) were collected from 15 rabbits in 6 prefectures. RESULTS: Characteristic histopathologic findings were observed, especially around the bile ducts, depending on the developmental stages of the parasites. Eimeria stiedae and E. flavescens were successfully identified by PCR and sequencing analyses in 5 liver samples and 1 cecum sample, respectively. CONCLUSION: Our results could enhance understanding of infection with Eimeria spp. in rabbits in Japan and contribute to pathological or molecular diagnoses.

DOI： 10.1007/s11686-023-00678-x

PubMed
A parasitological survey and the molecular detection of Entamoeba species in pigs of East Java, Indonesia

Chrismanto D.

Veterinary World 16 ( 3 ) 650 - 656 2023.03（ ISSN:09728988 ）

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DOI： 10.14202/vetworld.2023.650-656

PubMed
致死的下痢症の集団発生酪農場における複数種のクリプトスポリジウムの分子同定及びその感染動態と清浄化対策

河合愛美, 林みち子, 高橋和瑛, 芝原友幸, 笹井和美, 松林誠

日本獣医師会雑誌 76 ( 2 ) e18 - e27 2023.02（ ISSN:0446-6454 ）

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2020年に石川県の2農場で下痢症を呈した子牛が相次いで死亡した.種々の検査及び病理解析の結果,クリプトスポリジウムによる感染が原因である可能性が考えられた.18S rRNA及びActin遺伝子領域を標的としたPCR及びMultiplex-PCRを実施したところ,Cryptosporidium parvumの2つの遺伝子亜型による混合感染であることが分かった.これら2種の遺伝子亜型による混合感染は国内初めての報告である.同農場で飼育されていた他個体の調査では,感染は子牛に限られ,さらにC.bovis及びC.ryanaeが検出された.飼育環境の調査では子牛を飼育していた床の敷料のみから本原虫が検出され,汚染エリアを熱湯及び石灰乳の塗布により対策を実施したが,短期的には下痢を発症する頭数が減少し効果がみられたものの,清浄化には至らなかった.(著者抄録)
Reduction of oocyte shedding and cecal inflammation by 5-aminolevulinic acid daily supplementation in laying hens infected with Eimeria tenella. Reviewed

Toshimitsu Hatabu, Hung Hoang Son Pham, Wataru Aota, Shota Fujino, Rio Nishihara, Go Kawamura, Yuudai Sakogawa, Shin Taniguchi, Makoto Matsubayashi

Animal science journal 94 ( 1 ) e13806 2023.01（ ISSN:1344-3941 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

The present study aimed to evaluate the effects of 5-aminolevulinic acid (5-ALA) on Eimeria tenella infection in laying hens. Oocyst shedding and histopathology were evaluated. A reduced oocyst shedding was observed 5 and 7 days post-infection (dpi) in the 5-ALA-administered group, but the total number of oocysts during the first infection period was not different between control and 5-ALA-treated groups. After E. tenella attack infection, the period of oocyst shedding in the 5-ALA-administered group lasted less long than that in controls. During the attack infection period, the total number of fecal oocysts in the 5-ALA-treated group was significantly lower than that in the control group. However, the parasite burden score in hens receiving 5-ALA was higher than that in controls after E. tenella attack infection. The lesion scores at 5 and 30 dpi in the control group were significantly lower than those in the 5-ALA-administered group. Therefore, 5-ALA administration might be beneficial against E. tenella infection in laying hens.

DOI： 10.1111/asj.13806

PubMed
Evaluation of the host specificity of Eimeria uekii and Eimeria raichoi for Japanese rock ptarmigans by oocyst transfer to taxonomically related birds Reviewed

Makoto Matsubayashi, Sayaka Tsuchida, Atsushi Kobayashi, Tomoyuki Shibahara, Isao Teramoto, Yasutoshi Kido, Akira Kaneko, Hiroshi Nakamura, Masami Hasegawa, Kazumi Sasai, Kazunari Ushida

Parasitology Research 2023

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Authorship：Lead author,　Corresponding author Publishing type：Research paper (scientific journal)
Identifications and Distributions of Multiple Species and Genotypes of Cryptosporidium at Farms with Calf Deaths and Treatments against Parasites

KAWAI Megumi, HAYASHI Michiko, TAKAHASHI Kazuaki, SHIBAHARA Tomoyuki, SASAI Kazumi, MATSUBAYASHI Makoto

Journal of the Japan Veterinary Medical Association 76 ( 2 ) e18 - e27 2023（ ISSN:04466454 ）

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Five calves died at two farms in Ishikawa prefecture, Japan, in 2020. Pathological examinations revealed that these calves may have been infected with Cryptosporidium spp. PCR of 18S rRNA genes and a multiplex PCR of actin genes identified two genotypes of Cryptosporidium parvum, type 1 and type 2, which included 3 bp deletions and 2 bp substitutions, in all calves. This is the first report of mixed infections with multiple genotypes of Cryptosporidium. C. bovis and C. ryanae were also detected in other calves reared at the same farms. Environmental examinations of the farms by PCR revealed that Cryptosporidium spp. were present only in the rice husks that were used as bedding litter. The rate of animals with diarrhea was reduced by treating the areas in which the calves were reared with boiling water and milk of lime. However, the infections could not be completely eliminated.

DOI： 10.12935/jvma.76.e18
Molecular identification of Eimeria species in liver and feces of naturally infected rabbits in Japan. Reviewed

Kazue Katsui, Shigeaki Takami, Kousuke Ohashi, Hiromi Otsuka, Shigehiko Uni, Tomoyuki Shibahara, Kazumi Sasai, Makoto Matsubayashi

Parasitology research 121 ( 9 ) 2733 - 2738 2022.09（ ISSN:09320113 ）

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Authorship：Corresponding author Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Among the 11 species of Eimeria in rabbits, some of which are known to be pathogenic and cause enteritis, E. stiedae induces severe liver lesions resulting in elevated mortality. Unlike in other countries, the incidence and prevalence of the parasites in rabbits have not been reported in Japan. In the present study, we histopathologically analyzed hepatic coccidiosis in a rabbit and attempted several primers to genetically identify the parasites and investigated the prevalence of Eimeria species at the same farm. In the liver of the affected rabbit, we observed fibrosis and edema around multiple bile ducts and epithelial cell hyperplasia of the bile ducts. Large numbers of developing parasites of Eimeria spp., mainly oocysts, were present in the bile ducts. PCR and sequencing analyses with the published primers for Cyclospora and Eimeria spp. were used to successfully identify the parasites in the liver as E. stiedae. The oocysts of Eimeria spp. were detected in 13 out of 20 fecal samples collected from other rabbits at the farm, and five Eimeria spp. (E. perforans, E. flavescens, E. exigua, E. magna, and E. vejdovskyi) were genetically confirmed. Our results provide the first indication that Eimeria spp., including highly pathogenic species, are present in Japan and the primer set used herein can be a useful tool for the identification of rabbit Eimeria spp.

DOI： 10.1007/s00436-022-07580-x

PubMed
Synthesis and cytotoxic activities of 8- and 6-demethyleucalyptins(和訳中)

Asakawa Ryuki, Fuchiyama Kanta, Ishii Yunosuke, Hosaka Keisuke, Kobayashi Atsushi, Shimazaki Kei, Nagasawa Junki, Tsuchida Sayaka, Ushida Kazunari, Matsubayashi Makoto, Furuyama Yuuki, Ohgane Kenji, Kuramochi Kouji

Bioscience, Biotechnology, and Biochemistry 86 ( 9 ) 1200 - 1206 2022.09（ ISSN:0916-8451 ）

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ニホンライチョウが餌としているミネズオウ(Kalmia procumbens)からC-メチルフラボンを単離した。その構造は報告されたNMRデータとの比較により8-デメチルオイカリプチンと推定されたが、単離化合物が6-デメチルオイカリプチンである可能性を排除できなかった。そこで、二つの化合物を合成し比較した。単離化合物は8-デメチルオイカリプチンと明確に決定した。8-および6-デメチルオイカリプチンの細胞毒性を調べた。その結果、前者だけがHCT116とMRC-5細胞に対して細胞毒性を示した。
Synthesis and Cytotoxic Activities of 8- and 6-Demethyleucalyptins.

Ryuki Asakawa, Kanta Fuchiyama, Yunosuke Ishii, Keisuke Hosaka, Atsushi Kobayashi, Kei Shimazaki, Junki Nagasawa, Sayaka Tsuchida, Kazunari Ushida, Makoto Matsubayashi, Yuuki Furuyama, Kenji Ohgane, Kouji Kuramochi

Bioscience, biotechnology, and biochemistry 86 ( 9 ) 1200 - 1206 2022.07（ ISSN:09168451 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Secondary metabolites in plants influence the health of herbivores such as Japanese rock ptarmigans that feed on the leaves and fruits of alpine plants. Thus, it is important to understand the secondary metabolites of alpine plants and their biological activities for conserving Japanese rock ptarmigans. We isolated C-methylflavone from the leaves of Kalmia procumbens, on which Japanese rock ptarmigans feed. Although its structure was deduced to be 8-demethyleucalyptin by comparing its NMR data with the reported ones, the possibility that the isolated compound is 6-demethyleucalyptin cannot be ruled out. Thus, both isomers were synthesized. The isolated compound was unambiguously determined to be 8-demethyleucalyptin by comparing its NMR data with those of the synthetic ones. Cytotoxic evaluation of 8- and 6-demethyleucalyptins revealed that only the former showed cytotoxicity against HCT116 and MRC-5 cells. The present study provides not only easy access to 8- and 6-demethyleucalyptins, but also their biological information.

DOI： 10.1093/bbb/zbac105

PubMed
Ambivalent Roles of Oxidative Stress in Triangular Relationships among Arthropod Vectors, Pathogens and Hosts.

Emmanuel Pacia Hernandez, Anisuzzaman, Md Abdul Alim, Hayato Kawada, Kofi Dadzie Kwofie, Danielle Ladzekpo, Yuki Koike, Takahiro Inoue, Sana Sasaki, Fusako Mikami, Makoto Matsubayashi, Tetsuya Tanaka, Naotoshi Tsuji, Takeshi Hatta

Antioxidants (Basel, Switzerland) 11 ( 7 ) 2022.06（ ISSN:2076-3921 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Blood-feeding arthropods, particularly ticks and mosquitoes are considered the most important vectors of arthropod-borne diseases affecting humans and animals. While feeding on blood meals, arthropods are exposed to high levels of reactive oxygen species (ROS) since heme and other blood components can induce oxidative stress. Different ROS have important roles in interactions among the pathogens, vectors, and hosts. ROS influence various metabolic processes of the arthropods and some have detrimental effects. In this review, we investigate the various roles of ROS in these arthropods, including their innate immunity and the homeostasis of their microbiomes, that is, how ROS are utilized to maintain the balance between the natural microbiota and potential pathogens. We elucidate the mechanism of how ROS are utilized to fight off invading pathogens and how the arthropod-borne pathogens use the arthropods' antioxidant mechanism to defend against these ROS attacks and their possible impact on their vector potentials or their ability to acquire and transmit pathogens. In addition, we describe the possible roles of ROS in chemical insecticide/acaricide activity and/or in the development of resistance. Overall, this underscores the importance of the antioxidant system as a potential target for the control of arthropod and arthropod-borne pathogens.

DOI： 10.3390/antiox11071254

PubMed
Coproparasitological examinations and molecular determination of Eimeria species in Madura cattle reared on Madura Island, Indonesia

2022.02

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Authorship：Corresponding author Publishing type：Research paper (scientific journal)
インドネシアのMadura島で飼育されているMadura牛におけるEimeria種の便寄生虫検査および分子的決定(Coproparasitological examinations and molecular determination of Eimeria species in Madura cattle reared on Madura Island, Indonesia)

Poedji Hastutiek , Nunuk Dyah Retno Lastuti , Lucia Tri Suwanti , Agus Sunarso , Endang Suprihati , Dyah Ayu Kurniawati , Matsubayashi Makoto

Parasitology International 86 1 - 4 2022.02（ ISSN:1383-5769 ）
Evaluation of Cryptosporidium parvum oocyst inactivation following exposure to ultraviolet light-emitting diodes by in vitro excystation and dye staining assays Reviewed

Makoto Matsubayashi, Isao Teramoto, Itsuo Urakami, Jun Naohara, Kazumi Sasai, Yasutoshi Kido, Akira Kaneko

Parasitology International 88 102557 - 102557 2022

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Authorship：Lead author,　Corresponding author Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Cryptosporidium spp. are protozoan parasites that are transmitted via fecal-oral routes and can exhibit chemical resistance. Chlorine resistance makes it very difficult to eliminate parasites present in contaminated drinking water. While the efficacy of ultraviolet light-emitting diodes (UV-LEDs) against microorganisms has been reported, the efficacy of UV-LEDs against Cryptosporidium spp. has not been fully evaluated. Here, we assessed the efficacy of UV-LEDs with peak wavelengths of 268, 275, 284, and 289 nm against Cryptosporidium parvum at various exposure times, with a fixed exposure distance, using two in vitro methods. Consequently, the time required for 2 log10 inactivation through the excystation method by UV-LEDs of 268, 275, 284, and 289 nm was estimated as 115.5, 104.1, 37.4, and 30.7 min, respectively. The propidium iodide (PI) and 4',6-diamidino-2-phenylindole (DAPI) staining assays estimated the inactivation time as 311.3, 275.2, 60.6, and 39.1 min, respectively. Our results showed that UV-LED irradiation at longer wavelengths produced higher inactivation activity against C. parvum, which corroborates our previously reported in vivo assay results, although further study is needed to clarify the mechanism.

DOI： 10.1016/j.parint.2022.102557

PubMed
コクシジウムが関与した子牛の慢性壊死性腸炎

杉江建之介, 小松徹也, 川本尚未, 橋本幸江, 松林誠, 芝原友幸

家畜診療 68 ( 10 ) 591 - 597 2021.10（ ISSN:0287-0754 ）
Distribution of Eimeria uekii and Eimeria raichoi in cage protection environments for the conservation of Japanese rock ptarmigans (Lagopus muta japonica) in the Japanese Alps.

Makoto Matsubayashi, Atsushi Kobayashi, Minemitsu Kaneko, Moemi Kinoshita, Sayaka Tsuchida, Tomoyuki Shibahara, Masami Hasegawa, Hiroshi Nakamura, Kazumi Sasai, Kazunari Ushida

International journal for parasitology. Parasites and wildlife 15 225 - 230 2021.08

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Japanese rock ptarmigans, Lagopus muta japonica, are classified as an endangered species in Japan and are found only in the Japanese Alps. The number of birds has decreased in the last half century and cage protection projects have been undertaken as in situ conservation strategies (one of the projects for the recovery plan of Japanese rock ptarmigan) in the mountains. During the period with cage protections, some chicks died and two Eimeria spp., E. uekii and E. raichoi, were identified in the chicks. Here, we examined the soil within the cages and in the surrounding environment to assess potential sources of infection between July to August 2020. We found high numbers of oocysts in the cages, especially at the back sides where the ptarmigan family frequently congregated, but soils in other areas outside the cages were less contaminated or not contaminated at all. The time required for more than 50% of the oocysts to sporulate at 15, 20 and 25 °C for E. uekii was 20, 11, and 5 h, respectively, and 72, 48 and 18 h, respectively, for E. raichoi. Our results cast some doubt that coprophagia by chicks is the source of infection because chicks consumed fresh cecal feces (approximately within 1 h) as far as we know, and instead, the protected chicks might be directly or indirectly infected by oocysts in soils or the environment.

DOI： 10.1016/j.ijppaw.2021.05.004

PubMed
Morphological and molecular identification of Eimeria tetartooimia oocysts from a Japanese green pheasant (Galliformes; Phasianidae; Phasianus versicolor) at a zoo in Japan. Reviewed

Makoto Matsubayashi, Kazutoshi Takami, Moemi Kinoshita, Sayaka Tsuchida, Kazunari Ushida, Tomoyuki Shibahara, Kazumi Sasai

Parasitology research 120 ( 8 ) 2973 - 2979 2021.08

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Authorship：Lead author,　Corresponding author Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

We detected Eimeria oocysts from Japanese green pheasants (Phasianus versicolor) at a zoo in Osaka, Japan. The oocyst isolates were subspherical or ovoidal shaped and measured 17.2 (range 14.7-20.0) μm in length and 14.8 (13.3-16.7) μm in width with a length/width (L/W) ratio of 1.2 (1.0-1.4) and each had one polar granule. The oocysts lacked a residuum and micropyle. Sporocysts measured 9.8 (6.7-13.3) μm in length and 5.9 (4.7-7.3) μm in width, with a L/W ratio of 1.2 (1.1-1.4). Compared to previously published values, this strain shows morphological similarities with an isolate of E. teetartooimia from ring-necked pheasants from other countries. Phylogenetic analysis of the 18S rRNA and mitochondrial cytochrome c oxidase subunit I genes places the isolate in a clade related to chicken Eimeria spp., such as E. acervulina or E. brunetti. Although further analysis is needed, this information can be helpful for the diagnosis and determination of virulence of Eimeria spp. in pheasants.

DOI： 10.1007/s00436-021-07197-6

PubMed
鶏の免疫にかかわる細菌の研究進展. Reviewed

令和２(２０２１)年　杉山美樹，松林誠，永野哲司，落合絢子，岡村雅史，斉藤恵子，笛吹達史.

鶏病研究会報雑誌 56 ( 4 ) 2021.02

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Kind of work：Joint Work
キジ科に寄生するEimeria 属原虫の形態学的特徴とその比較

木下萌美、芝原友幸、笹井和美、松林誠.

鶏病研究会報雑誌 56 ( 4 ) 2021.02

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Kind of work：Joint Work
Phylogenetic characterization of Isospora jaracimrmani oocysts from a veiled chameleon (family Chamaeleonidae; Chamaeleo calyptratus) reared at a zoo in Ishikawa, Japan. Reviewed

Fitrine Ekawasti, Kazuya Kitagawa, Hiroshi Domae, April Hari Wardhana, Junki agasawa, Tomoyuki Shibahara, Masaharu Tokoro, Kazumi Sasai, Makoto Matsubayashi

J Vet Med Sci 2021

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Kind of work：Joint Work
Reduction of macrophages by carrageenan decreases oocyst output and modifies local immune reaction in chick cecum with Eimeria tenella. Reviewed

Dung Thi Ho, Hung Hoang Son Pham, Wataru Aota, Makoto Matsubayashi, Naotoshi Tsuji, Toshimitsu Hatabu

Research in veterinary science 139 59 - 66 2021

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

This study aimed to evaluate the disease severity and local immune responses in macrophage-depleted chicks with Eimeria tenella. Macrophages were reduced by intraperitoneal injection of a carrageenan solution at 12, 13, and 16 days old, whereas the control group received intraperitoneal phosphate-buffered saline. Both chick groups were orally inoculated with E. tenella sporulated oocysts at 14 days old. Feces were collected daily, which were then quantified for oocysts. The chicks were sacrificed on day 5, and the ceca were collected for histopathological observation. The gene expression levels were measured using real-time quantitative reverse transcription-polymerase chain reaction. Macrophage-depleted chicks have been observed to shed a significantly reduced number of fecal oocysts compared to the infected control group. The parasite burden score in cecum specimens of macrophage-depleted chicks was significantly lower than those of infected control on day 5 after infection. Furthermore, macrophage reduction yielded significantly lower cecum histopathological scores and CD4 expression than those of the infected control group. The expression of interleukin (IL)-18, IL-22, interferon-γ, and inducible nitric oxide synthase was also noted to be significantly upregulated in both infected control and macrophage-depleted chicks compared to uninfected chicks. IL-4, IL-13, IL-17, and perforin expressions were also higher with macrophage depletion than in both control groups. These results suggest that macrophages serve as an invasive gate or a transporting vehicle to the site of first merogony. Furthermore, mononuclear phagocytes may play an important role in local immune responses, thus contributing to parasite development during early E. tenella infection.

DOI： 10.1016/j.rvsc.2021.07.003

PubMed
Relationship between Eimeria tenella associated-early clinical signs and molecular changes in the intestinal barrier function. Reviewed

Hung Hoang Son Pham, Makoto Matsubayashi, Naotoshi Tsuji, Toshimitsu Hatabu

Veterinary immunology and immunopathology 240 110321 - 110321 2021

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

The major clinical signs of coccidiosis in chickens due to Eimeria parasite are diarrhea and bloody feces. Previous studies showed that the impairment of the intestinal epithelial barrier and the elevation of the intestinal permeability are causes of clinical signs associated with coccidia challenges. Nevertheless, the information about molecular changes of the epithelial barrier at the early stage of the infection with a specific Eimeria species has not been mentioned. Hence, this study aims to elucidate the temporal relationships between epithelial barrier conditions and clinical signs in chickens infected with Eimeria tenella over the time from the earliest stages of infection. White Leghorn chickens were inoculated with 1 × 104 oocysts of E. tenella. Thereafter the chickens were monitored for their daily clinical signs through observation, and between 5 dpi to 10 dpi, feces were collected for oocysts counting. Chickens were then administrated with fluorescein isothiocyanate-dextran (FITC-d) for gastrointestinal permeability test and tissues were collected each day for histopathological observation and total RNA extraction. Finally, the mRNA expression levels of the tight and adherens junction genes and cytokine genes were evaluated using the quantitative real-time polymerase chain reaction (qRT-PCR). In this study, clinical signs such as diarrhea and bloody feces were observed concurrently from 3 to 8 dpi. Histopathology changes such as severe inflammation, hemorrhage, and epithelial desquamation were identified in the cecum specimens. The FITC-d level in the E. tenella-infected group was significantly higher than in the control group. In the infected group, the expression of claudin-2 gene was also upregulated, whereas the expressions of claudin-3 and E-cadherin genes were decreased as compared to the control group. These results implied that clinical signs of avian coccidiosis were associated with the intestinal barrier disruption via changes in expression levels of claudins and E-cadherin at the intestine.

DOI： 10.1016/j.vetimm.2021.110321

PubMed
Phylogenetic characterization of Isospora jaracimrmani oocysts from a veiled chameleon (family Chamaeleonidae; Chamaeleo calyptratus) reared at a zoo in Ishikawa, Japan Reviewed

Fitrine EKAWASTI, Kazuya KITAGAWA, Hiroshi DOMAE, April Hari WARDHANA, Junki NAGASAWA, Tomoyuki SHIBAHARA, Masaharu TOKORO, Kazumi SASAI, Makoto MATSUBAYASHI

Journal of Veterinary Medical Science 2021（ ISSN:0916-7250 ）

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Publishing type：Research paper (scientific journal)

DOI： 10.1292/jvms.21-0152
Structural and Biochemical Features of Eimeria tenella Dihydroorotate Dehydrogenase, a Potential Drug Target.

Dan Sato, Endah Dwi Hartuti, Daniel Ken Inaoka, Takaya Sakura, Eri Amalia, Madoka Nagahama, Yukina Yoshioka, Naotoshi Tsuji, Tomoyoshi Nozaki, Kiyoshi Kita, Shigeharu Harada, Makoto Matsubayashi, Tomoo Shiba

Genes 11 ( 12 ) 2020.12

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Dihydroorotate dehydrogenase (DHODH) is a mitochondrial monotopic membrane protein that plays an essential role in the pyrimidine de novo biosynthesis and electron transport chain pathways. In Eimeria tenella, an intracellular apicomplexan parasite that causes the most severe form of chicken coccidiosis, the activity of pyrimidine salvage pathway at the intracellular stage is negligible and it relies on the pyrimidine de novo biosynthesis pathway. Therefore, the enzymes of the de novo pathway are considered potential drug target candidates for the design of compounds with activity against this parasite. Although, DHODHs from E. tenella (EtDHODH), Plasmodium falciparum (PfDHODH), and human (HsDHODH) show distinct sensitivities to classical DHODH inhibitors, in this paper, we identify ferulenol as a potent inhibitor of both EtDHODH and HsDHODH. Additionally, we report the crystal structures of EtDHODH and HsDHODH in the absence and presence of ferulenol. Comparison of these enzymes showed that despite similar overall structures, the EtDHODH has a long insertion in the N-terminal helix region that assumes a disordered configuration. In addition, the crystal structures revealed that the ferulenol binding pocket of EtDHODH is larger than that of HsDHODH. These differences can be explored to accelerate structure-based design of inhibitors specifically targeting EtDHODH.

DOI： 10.3390/genes11121468

PubMed
Structural and biochemical features of Eimeria tenella dihydroorotate dehydrogenase, a potential drug target

Dan Sato, Endah Dwi Hartuti, Daniel Ken Inaoka, Takaya Sakura, Eri Amalia, Madoka Nagahama, Yukina Yoshioka, Naotoshi Tsuji, Tomoyoshi Nozaki, Kiyoshi Kita, Shigeharu Harada, Makoto Matsubayashi and Tomoo Shiba

Genes (Basel) 11 ( 12 ) 2020.12

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Kind of work：Joint Work
Structural and Biochemical Features of Eimeria tenella Dihydroorotate Dehydrogenase, a Potential Drug Target.

Sato D, Hartuti ED, Inaoka DK, Sakura T, Amalia E, Nagahama M, Yoshioka Y, Tsuji N, Nozaki T, Kita K, Harada S, Matsubayashi M, Shiba T.

Genes (Basel) 11 ( 12 ) 2020.12

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Kind of work：Single Work
Occurrence and genetic identifications of porcine Entamoeba, E. suis and E. polecki, at Tangerang in West Java, Indonesia

April Hari Wardhana, Dyah Haryuningtyas Sawitri, Fitrine Ekawasti, Eny Martindah, Dias Apritadewi, Tomoyuki Shibahara, Masahiro Kusumoto, Masaharu Tokoro, Kazumi Sasai, Makoto Matsubayashi

Parasitology Research 雑誌 119 ( 9 ) 2983 - 2990 2020.09
Efficacy of ultraviolet light-emitting diodes (UV-LED) at four different peak wavelengths against Cryptosporidium parvum oocysts by inactivation assay using immunodeficient mice. Reviewed

Karin Takahashi, Makoto Matsubayashi, Yukio Ohashi, Jun Naohara, Itsuo Urakami, Kazumi Sasai, Yasutoshi Kido, Akira Kaneko, Isao Teramoto

Parasitology international 77 102108 - 102108 2020.08

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

As an alternative to using ultraviolet (UV) lamps, which are made with mercury that is toxic to the environment and human health, UV light-emitting diodes (UV-LEDs) are expected to be effective for inactivating microorganisms in water. Although UV-LEDs have been reported to be effective against bacteria and viruses, the effectiveness of UV-LEDs against Cryptosporidium parasites has not been fully evaluated. As we report here, we have developed an in vivo quantitative inactivation assay for C. parvum oocysts using immunodeficient mice. Using the assay, we evaluated the effectiveness of treatment by UV lamp (254 nm) at approximately 1000 μJ/cm2 (for 3 s at a distance of 95 mm) compared to inactivation by commercially available UV-LEDs (with peak wavelengths of 268, 275, 284, and 289 nm). The shed patterns of oocysts after treatment with 284- and 289-nm wavelength UV-LEDs were significantly delayed compared to that after treatment with a UV lamp. These findings provide the first suggestion that UV-LEDs are effective against these parasites, as assessed using commercially available 350-mA UV-LEDs under conditions of fixed exposure distance and time.

DOI： 10.1016/j.parint.2020.102108

PubMed
子豚の小腸及び大腸パラフィン包埋切片を用いたTritrichomonas foetusの遺伝子学的同定及び粘膜固有層への侵入の特徴

猜都勇介, 北原尚英, 三角和華子, 伊藤弘貴, 松林誠, 笹井和美, 芝原友幸

日本獣医師会雑誌 73 ( 8 ) 449 - 456 2020.08（ ISSN:0446-6454 ）

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鹿児島県内の一養豚農場で2019年2月に40日齢の離乳豚が水様性下痢を呈し、死亡豚が増加した。下痢を呈した2頭において、病理組織学的に回腸、盲腸及び結腸の陰窩腔にトリコモナスの濃厚寄生が観察された。そのうち1頭では、多数のトリコモナスの陰窩上皮及び粘膜固有層への侵入が認められた。小腸及び大腸病変部のパラフィン包埋切片を用いたPCR検査でTritrichomonas foetusが同定された。超微形態学的検査で、3本の前鞭毛と1本の後鞭毛が観察された。Caspase-3免疫染色及びTUNEL法で、陰窩上皮のアポトーシス陽性細胞の増加が観察された。細菌学的検査でEscherichia coliが分離された。病理組織学的検査と遺伝子学的検査の結果より、T.foetusの豚に対する侵襲性及び下痢への関与が示唆された。(著者抄録)
免疫不全マウスを用いた不活化アッセイによるCryptosporidium parvumオーシストに対する4種類のピーク波長での紫外線発光ダイオード(UV-LED)の有効性(Efficacy of ultraviolet light-emitting diodes(UV-LED) at four different peak wavelengths against Cryptosporidium parvum oocysts by inactivation assay using immunodeficient mice)

Takahashi Karin, Matsubayashi Makoto, Ohashi Yukio, Naohara Jun, Urakami Itsuo, Sasai Kazumi, Kido Yasutoshi, Kaneko Akira, Teramoto Isao

Parasitology International 77 1 - 6 2020.08（ ISSN:1383-5769 ）

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各種波長のUV-LEDによるCryptosporidium parvum不活化作用を、重症複合免疫不全症(SCID)マウスを用いて評価した。5週齢の雌SCIDマウスを7群に割り付けた(5～6頭/群)。A群には未処理オーシスト10^5、B群には未処理オーシスト10^3、C群にはUVランプ照射(波長253.7nm)オーシスト10^6、D～G群にはUV-LED(ピーク波長268、275、284、289nm)照射オーシスト10^6を腹腔内投与した。1日当たりのオーシスト排出量(OPD)を算出した。全ての処理群のOPD値は対照(未処理オーシスト)群と同様に、10^7のプラトーに達した。未処理オーシスト群の増殖曲線の標準偏差は比較的小さかったのに対して、UV/UV-LED処理オーシスト群では大きな分散を示した。OPDが10^4、10^5、10^6に達する推定日数については、波長284nmと289nmのUV-LEDで処理した場合の方がUVランプ処理の場合より有意に長かった。10^3対照群における40日後の死亡率は0%、10^5対照群で66.7%(4/6)、UVランプ群では40%(2/5)、UV-LED群では波長268nmが60%(3/5)、275nmが0%、284nmが20%(1/5)、289nmが0%であった。波長約280nmのUV-LED照射はオーシストからの脱嚢を阻害するものと推測された。
Parasitic development in intestines and oocyst shedding patterns for infection by Eimeria uekii and Eimeria raichoi in Japanese rock ptarmigans, Lagopus muta japonica, protected by cages in the Southern Japanese Alps. Reviewed

Makoto Matsubayashi, Moemi Kinoshita, Atsushi Kobayashi, Sayaka Tsuchida, Tomoyuki Shibahara, Masami Hasegawa, Hiroshi Nakamura, Kazumi Sasai, Kazunari Ushida

International journal for parasitology. Parasites and wildlife 12 19 - 24 2020.08

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

The population of Japanese rock ptarmigan (Lagopus muta japonica), an endangered species with a habitat above the timberline of the southern Japanese Alps, has declined. As one of the recent conservation strategies for this species, cage protection for broods (hens and chicks) has been introduced in their habitats. Two species of Eimeria have frequently been detected in these birds, but little is known about the parasitic circulation in the region, including among birds and in the environment. Here, we conducted histopathology examinations of dead chicks collected under cage protection in 2018, and examined the feces of the hens and chicks of three broods and environmental soils for parasites in 2019 in order to assess the potential sources of infection and pathogenicity. Developmental zoites were found in the epithelial mucosa and/or the submucosa from the duodenum to the colon of all dead chicks. Fecal examination revealed oocysts of E. uekii and/or E. raichoi in all hens and chicks. Oocysts of Eimeria spp. per gram of feces in chicks increased within 2 weeks after hatching and then gradually deceased. Following infection of the chicks, oocysts could accumulate within the cage areas, and oocyst density exceeded more than 1000 oocysts per gram of cage soils. Based on having sporulated morphologies, oocysts could be infective and therefore, be direct or indirect potential sources of infection. However, based on our findings that not all chicks were clinically affected by the infections, other factors such as microbial flora in the chicks established by coprophagy or from the habitat environment, including climate, might be associated with the pathogenicity of Eimeria spp., although further studies are needed to assess these correlations.

DOI： 10.1016/j.ijppaw.2020.04.002

PubMed
Occurrence and genetic identifications of porcine Entamoeba, E. suis and E. polecki, at Tangerang in West Java, Indonesia. Reviewed

April Hari Wardhana, Dyah Haryuningtyas Sawitri, Fitrine Ekawasti, Eny Martindah, Dias Apritadewi, Tomoyuki Shibahara, Masahiro Kusumoto, Masaharu Tokoro, Kazumi Sasai, Makoto Matsubayashi

Parasitology research 119 ( 9 ) 2983 - 2990 2020.07

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Entamoeba suis and E. polecki subtype (ST) 1 and ST3 recently have been inferred to be virulent in pigs. However, because relevant molecular epidemiological surveys have been limited, the prevalences of these species remain unknown and their pathogenicities are still controversial. We surveyed 196 fecal samples of pigs (118 of adults, 78 of piglets) at Tangerang in West Java, Indonesia, in 2017, employing PCR using porcine Entamoeba-specific primers. E. suis was the more frequently detected species, observed in 81.1% of samples, while E. polecki ST1 and ST3 were detected in 18.4% and 17.3% of samples, respectively; mixed infections (harboring 2-3 species or subtypes of Entamoeba) were confirmed in 29.3% of positive samples. Statistically significant differences in the positive rates were not seen between adult pigs and piglets, except for those of E. polecki ST3. The prevalences of Eimeria spp. and/or Cystoisospora suis (79.1%), strongyles (55.6%), and Strongyloides spp. (6.1%) were also observed morphologically in the samples. Further chronological or seasonal investigations of pigs and humans in these high-prevalence areas are needed to assess the virulence of the Entamoeba parasites, including the effects on pig productivity, and to evaluate the zoonotic impacts of these organisms.

DOI： 10.1007/s00436-020-06806-0

PubMed
Molecular identification of Trichuris suis worms and eggs in pig feces, infected intestines, and farm environments in Japan Reviewed

Muramatsu R, Sato R, Onuma N, Sasai K, Shibahara T, Matsubayashi M

Jarq-Japan Agricultural Research Quarterly 54 ( 3 ) 2020.07
Morphological and molecular identification of Eimeria spp. in breeding chicken farms of Japan. Reviewed

Makoto Matsubayashi, Tomoyuki Shibahara, Tomohide Matsuo, Toshimitsu Hatabu, Junya Yamagishi, Kazumi Sasai, Takashi Isobe

The Journal of veterinary medical science 82 ( 5 ) 516 - 519 2020.05

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Publishing type：Research paper (scientific journal) International / domestic magazine：Domestic journal

There have been no reports of the prevalence of Eimeria spp. in poultry breeding farms in Japan unlike those of broiler farms. From 2017 to 2018, we examined the prevalence of Eimeria spp. on breeding farms in Japan by oocyst morphology and PCR analyses. A total of 143 samples was collected from 37 breeding farms in 21 prefectures of Japan. We detected oocysts of seven species at 34 of 37 breeding farms by PCR, and we identified E. brunetti at 51.5% of farms found to be positive for Eimeria. The differences in the identification of Eimeria spp. between the morphology and PCR assay methods of oocysts were pronounced for E. maxima and E. necatrix. We confirmed that molecular tools were more suitable for accurately estimating prevalence of Eimeria spp., and these findings suggest that E. brunetti could be widespread in Japan.

DOI： 10.1292/jvms.19-0661

PubMed
日本の繁殖養鶏場におけるEimeria属の形態学的及び分子学的同定(Morphological and molecular identification of Eimeria spp. in breeding chicken farms of Japan)

Matsubayashi Makoto, Shibahara Tomoyuki, Matsuo Tomohide, Hatabu Toshimitsu, Yamagishi Junya, Sasai Kazumi, Isobe Takashi

The Journal of Veterinary Medical Science 82 ( 5 ) 516 - 519 2020.05（ ISSN:0916-7250 ）
Swine multifocal ulcerative colitis and crypt abscesses associated with Entamoeba polecki subtype 3 and Salmonella Typhimurium. Reviewed

Hirotaka Ito, Kumiko Hosokawa, Midori Kawamura, Naomi Ito, Yusuke Abeto, Makoto Matsubayashi, Kazumi Sasai, Tomoyuki Shibahara

The Journal of veterinary medical science 82 ( 4 ) 463 - 466 2020.04

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Publishing type：Research paper (scientific journal) International / domestic magazine：Domestic journal

Piglets aged approximately 50 days exhibited diarrhea and wasting. Multiple white foci were detected in the colon of a dead piglet; histopathological findings revealed multifocal ulcers and crypt abscesses with Entamoeba trophozoites and gram-negative bacilli in the piglet. These pathogens were identified as Entamoeba polecki subtype 3 and Salmonella enterica serovar Typhimurium, respectively. Numerous E. polecki subtype 3 trophozoites were located on the edge of the ulcerative and necrotic lesions in the lamina propria. Crypt abscesses were associated with S. Typhimurium. These results suggest that E. polecki subtype 3 caused multifocal ulcerative colitis accompanied by crypt abscesses with S. Typhimurium in the piglet. This study is the first report of colitis with E. polecki subtype 3 and S. Typhimurium coinfection.

DOI： 10.1292/jvms.19-0682

PubMed
Molecular identification of Eimeria hestermani and Eimeria prionotemni from a red-necked wallaby (Macropodidae; Macropus rufogriseus) in Japan

Fitrine Ekawasti, Kazuya Kitagawa, Hiroshi Domae, April Hari Wardhana, Tomoyuki Shibahara, Shigehiko Uni, Masaharu Tokoro, Kazumi Sasai, Makoto Matsubayashi.

Parasitology Research 119 ( 4 ) 1271 - 1279 2020.04

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Kind of work：Joint Work
Parasitic development in intestines and oocyst shedding patterns for infection by Eimeria uekii and Eimeria raichoi in Japanese rock ptarmigans, Lagopus muta japonica, protected by cages in the Southern Japanese Alps

Makoto Matsubayashi, Moemi Kinoshita, Atsushi Kobayashi, Sayaka Tsuchida, Tomoyuki Shibahara, Masami Hasegawa, Hiroshi Nakamura, Kazumi Sasai, Kazunari Ushida.

International Journal for Parasitology: Parasites and Wildlife. 雑誌 12 19 - 24 2020.04

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Kind of work：Joint Work
Entamoeba poleckiサブタイプ3とSalmonella Typhimuriumに関連した豚の多病巣性潰瘍性大腸炎と陰窩膿瘍(Swine multifocal ulcerative colitis and crypt abscesses associated with Entamoeba polecki subtype 3 and Salmonella Typhimurium)

Ito Hirotaka, Hosokawa Kumiko, Kawamura Midori, Ito Naomi, Abeto Yusuke, Matsubayashi Makoto, Sasai Kazumi, Shibahara Tomoyuki

The Journal of Veterinary Medical Science 82 ( 4 ) 463 - 466 2020.04（ ISSN:0916-7250 ）
Molecular identification of Eimeria hestermani and Eimeria prionotemni from a red-necked wallaby (Macropodidae; Macropus rufogriseus) in Japan. Reviewed

Fitrine Ekawasti, Kazuya Kitagawa, Hiroshi Domae, April Hari Wardhana, Tomoyuki Shibahara, Shigehiko Uni, Masaharu Tokoro, Kazumi Sasai, Makoto Matsubayashi

Parasitology research 119 ( 4 ) 1271 - 1279 2020.04

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

To date, more than 50 Eimeria spp. have been isolated from marsupials of the family Macropodidae. Although 18 species of Eimeria have been previously detected from multiple animal species belonging to the genus Macropus of the family, limited genetic analyses of the parasites are available, and their pathogenicity remains unclear. Here, we report the isolation of Eimeria spp. from a zoo specimen of red-necked wallaby (Macropodidae; Macropus rufogriseus). Specifically, two distinct types of Eimeria oocysts were recovered, one from the feces before treatment with an anthelmintic and the second from the intestinal contents after death of the animal. The oocysts obtained from the two sources were morphologically identified as E. hestermani and E. prionotemni, respectively. We successfully determined partial gene sequences from the two isolates, including segments of the 18S rRNA genes, and for the first time have used phylogenetic analyses of these sequences to assign the species to distinct clades. In combination with further genetic data, these results are expected to help elucidate the pathogenicity and host ranges of Eimeria spp. within the respective family and genus.

DOI： 10.1007/s00436-020-06618-2

PubMed
Detections of gastrointestinal parasites, including Giardia intestinalis and Cryptosporidium spp., in cattle of Banten province, Indonesia. Reviewed

Dyah Haryuningtyas Sawitri, April Hari Wardhana, Eny Martindah, Fitrine Ekawasti, Dias Aprita Dewi, Bambang Ngaji Utomo, Tomoyuki Shibahara, Masahiro Kusumoto, Masaharu Tokoro, Kazumi Sasai, Makoto Matsubayashi.

Journal of Parasitic Diseases. 雑誌 44 ( 1 ) 174 - 179 2020.03

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Detections of gastrointestinal parasites, including Giardia intestinalis and Cryptosporidium spp., in cattle of Banten province, Indonesia. Reviewed

Dyah Haryuningtyas Sawitri, April Hari Wardhana, Eny Martindah, Fitrine Ekawasti, Dias Aprita Dewi, Bambang Ngaji Utomo, Tomoyuki Shibahara, Masahiro Kusumoto, Masaharu Tokoro, Kazumi Sasai, Makoto Matsubayashi

Journal of parasitic diseases : official organ of the Indian Society for Parasitology 44 ( 1 ) 174 - 179 2020.03

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International / domestic magazine：International journal

Gastrointestinal parasites can induce low productivity in livestock by causing acute or chronic enteritis. Veterinarians make great efforts to design rational and effective hygienic protocols for both the prevention and treatment of diarrhea. Although prevalences can vary depending on the examined areas or the ages of the hosts, and the methods used for detections, it is helpful to accumulate data across many areas to evaluate parasitic distribution. A coprological survey in cattle was conducted in Tangerang, Banten Province of Indonesia, in order to determine the prevalence of the parasites, including those of diarrhea-associated diseases. Furthermore, the risk of transmission of Giardia intestinalis and Cryptosporidium spp. to human was genetically analyzed. Gastrointestinal parasites were detected in 87 of 109 cattle samples, including 85 carrying Eimeria spp., 36 carrying Fasciola gigantica, 35 carrying Strongyloides spp., 33 carrying Paramphistomum spp., and 15 carrying Capillaria spp. Giardia intestinalis and Cryptosporidium spp., parasites with zoonotic potential, were detected in 9 and 1 cattle samples, respectively. Molecular analyses identified the G. intestinalis isolate as a member of Assemblage E, which has been recently detected in humans in another country. These results may be helpful in understanding the hygienic risk affecting the livestock productivity and zoonotic potential of cattle in Indonesia.

DOI： 10.1007/s12639-019-01179-3

PubMed
Description, molecular characteristics and Wolbachia endosymbionts of Onchocerca borneensis Uni, Mat Udin & Takaoka n. sp. (Nematoda: Filarioidea) from the Bornean bearded pig Sus barbatus Müller (Cetartiodactyla: Suidae) of Sarawak, Malaysia. Reviewed

Shigehiko Uni, Ahmad Syihan Mat Udin, Takeshi Agatsuma, Kerstin Junker, Weerachai Saijuntha, Naruemon Bunchom, Masako Fukuda, Coralie Martin, Emilie Lefoulon, Amandine Labat, Faisal Ali Anwarali Khan, Van Lun Low, Phaik Leng Cheah, Yvonne Ai-Lian Lim, Rosli Ramli, Daicus Martin Belabut, Nur Afiqah Zainuri, Makoto Matsubayashi, Hasmahzaiti Omar, Subha Bhassu, Shoji Uga, Rosli Hashim, Hiroyuki Takaoka, Mohd Sofian Azirun

Parasites & vectors 13 ( 1 ) 50 - 50 2020.02

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

BACKGROUND: The genus Onchocerca Diesing, 1841 includes species of medical importance, such as O. volvulus (Leuckart, 1893), which causes river blindness in the tropics. Recently, zoonotic onchocercosis has been reported in humans worldwide. In Japan, O. dewittei japonica Uni, Bain & Takaoka, 2001 from wild boars is a causative agent for this zoonosis. Many filarioid nematodes are infected with Wolbachia endosymbionts which exhibit various evolutionary relationships with their hosts. While investigating the filarial fauna of Borneo, we discovered an undescribed Onchocerca species in the bearded pig Sus barbatus Müller (Cetartiodactyla: Suidae). METHODS: We isolated Onchocerca specimens from bearded pigs and examined their morphology. For comparative material, we collected fresh specimens of O. d. dewittei Bain, Ramachandran, Petter & Mak, 1977 from banded pigs (S. scrofa vittatus Boie) in Peninsular Malaysia. Partial sequences of three different genes (two mitochondrial genes, cox1 and 12S rRNA, and one nuclear ITS region) of these filarioids were analysed. By multi-locus sequence analyses based on six genes (16S rDNA, ftsZ, dnaA, coxA, fbpA and gatB) of Wolbachia, we determined the supergroups in the specimens from bearded pigs and those of O. d. dewittei. RESULTS: Onchocerca borneensis Uni, Mat Udin & Takaoka n. sp. is described on the basis of morphological characteristics and its genetic divergence from congeners. Molecular characteristics of the new species revealed its close evolutionary relationship with O. d. dewittei. Calculated p-distance for the cox1 gene sequences between O. borneensis n. sp. and O. d. dewittei was 5.9%, while that between O. d. dewittei and O. d. japonica was 7.6%. No intraspecific genetic variation was found for the new species. Wolbachia strains identified in the new species and O. d. dewittei belonged to supergroup C and are closely related. CONCLUSIONS: Our molecular analyses of filarioids from Asian suids indicate that the new species is sister to O. d. dewittei. On the basis of its morphological and molecular characteristics, we propose to elevate O. d. japonica to species level as O. japonica Uni, Bain & Takaoka, 2001. Coevolutionary relationships exist between the Wolbachia strains and their filarial hosts in Borneo and Peninsular Malaysia.

DOI： 10.1186/s13071-020-3907-8

PubMed
Swine multifocal ulcerative colitis associated with Entamoeba polecki subtype 3 and Salmonella Typhimurium

Hirotaka ITO, Kumiko HOSOKAWA, Midori KAWAMURA, Naomi ITO, Yusuke ABETO, Makoto MATUBAYASHI, Kazumi SASAI, Tomoyuki SHIBAHARA.

J Vet Med Sci. 2020
Efficacy of ultraviolet light-emitting diodes (UV-LED) at four different peak wavelengths against Cryptosporidium parvum oocysts by inactivation assay using immunodeficient mice.

Karin Takahashi, Makoto Matsubayashi, Yukio Ohashi, Jun Naohara, Itsuo Urakami, Kazumi Sasai, Yasutoshi Kido, Akira Kaneko, Isao Teramoto.

Parasitology International. 雑誌 2020

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Morphological and molecular identification of Eimeria spp. in breeding chicken farms of Japan.

Makoto Matsubayashi, Tomoyuki Shibahara, Tomohide Matsuo, Toshimitsu Hatabu, Junya Yamagishi, Kazumi Sasai, Takashi Isobe.

J Vet Med Sci 著書 2020

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Kind of work：Joint Work
子豚の小腸及び大腸パラフィン包埋切片を用いたTritrichomonas foetusの遺伝子学的同定及び粘膜固有層への侵入の特徴 Reviewed

猜都勇介，北原尚英，三角和華子，伊藤弘貴，松林誠，笹井和美，芝原友幸

日本獣医師会雑誌雑誌 2020

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Kind of work：Joint Work
Description, molecular characteristics and Wolbachia endosymbionts of Onchocerca borneensis Uni, Mat Udin & Takaoka n. sp. (Nematoda: Filarioidea) from the Bornean bearded pig Sus barbatus Müller (Cetartiodactyla: Suidae) of Sarawak, Malaysia. Reviewed

Uni S, Mat Udin AS, Agatsuma T, Junker K, Saijuntha W, Bunchom N, Fukuda M, Martin C, Lefoulon E, Labat A, Khan FAA, Low VL, Cheah PL, Lim YA, Ramli R, Belabut DM, Zainuri NA, Matsubayashi M, Omar H, Bhassu S, Uga S, Hashim R, Takaoka H, Azirun MS.

Parasit Vectors. 2020

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Kind of work：Joint Work
Molecular identification of Trichuris suis worms and eggs in pig feces, infected intestines, and farm environments in Japan.

Ryunosuke Muramatsu, Ryo Sato, Narihisa Onuma, Kazumi Sasai, Tomoyuki Shibahara, Makoto Matsubayashi

Japan Agricultural Research Quarterly 雑誌 2020

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Kind of work：Joint Work
Transitions in morphological forms and rapid development of the asexual schizonts of Eimeria tenella through serial passaging in chicks

Makoto Matsubayashi, Hirotaka Yamaguchi, Takeshi Hatta, Fumiya Kawahara, Toshimitsu Hatabu, Hiroshi Iseki, Junya Yamagishi, Takashi Isobe, Isao Teramoto, Akira Kaneko, Kiyoshi Kita, Naotoshi Tsuji, Kazumi Sasai

Infection, Genetics and Evolution 雑誌 75 2019.11

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Kind of work：Joint Work
Transitions in morphological forms and rapid development of the asexual schizonts of Eimeria tenella through serial passaging in chicks. Reviewed

Makoto Matsubayashi, Hiroki Yamaguchi, Takeshi Hatta, Fumiya Kawahara, Toshimitsu Hatabu, Hiroshi Iseki, Junya Yamagishi, Takashi Isobe, Isao Teramoto, Akira Kaneko, Kiyoshi Kita, Naotoshi Tsuji, Kazumi Sasai

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 75 103993 - 103993 2019.11

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Attenuated strains of avian Eimeria parasites, generated by the selection of precocious lines through serial passaging in chicks, have been used widely as live vaccines. Detailed morphological transitions including their life cycle depending on the passages remain poorly understood. Here, we showed early development and acceleration of transitions in morphological forms of the asexual schizonts of E. tenella that had been attenuated for virulence by serial passaging. Our results may be helpful in understanding parasitism, facilitating further molecular analyses such as comparative genomic or transcriptomic tests.

DOI： 10.1016/j.meegid.2019.103993

PubMed
Molecular characterization of highly pathogenic Eimeria species among beef cattle on Java Island, Indonesia. Reviewed

Fitrine Ekawasti, Wisnu Nurcahyo, April Hari Wardhana, Tomoyuki Shibahara, Masaharu Tokoro, Kazumi Sasai, Makoto Matsubayashi.

Parasitology International 雑誌 2019.10

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Kind of work：Joint Work
Molecular characterization of highly pathogenic Eimeria species among beef cattle on Java Island, Indonesia. Reviewed

Fitrine Ekawasti, Wisnu Nurcahyo, April Hari Wardhana, Tomoyuki Shibahara, Masaharu Tokoro, Kazumi Sasai, Makoto Matsubayashi

Parasitology international 72 101927 - 101927 2019.10

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Gastrointestinal parasites including Eimeria spp. are known to affect domestic animal productivity causing watery or lethal bloody diarrhea. However, there are few reports on the detailed distribution of bovine Eimeria spp. in cattle, particularly in developing tropical and sub-tropical areas. Using a total of 289 fecal samples collected from beef cattle on Java Island, one of the five main islands of Indonesia, fecal examinations by the Whitlock and sugar flotation methods and molecular surveys were conducted to reveal the prevalence of 6 Eimeria spp. As a result of morphological screening using Whitlock methods and sugar flotation, Eimeria spp. prevalences of 9.4% and 52.3% were confirmed, respectively. The prevalence was higher in younger cattle [under 1 year (63.9%), 1-2 years (75.0%) and more than in 2 year old cattle (42.3%)]. The prevalences of identified species were as follows: 10.4% for E. bovis, 2.8% for E. ellipsoidalis, 2.1% for E. alabamensis, 1.4% for E. zuernii, 1.1% for E. auburnensis, and 0.4% for E. cylindrica. Moreover, prevalences of 12.8% for Strongyloides papillosus, 7.3% for Trichuris globulosa, and 0.3% for Capillaria bovis were detected. Although the average number of oocysts per gram of feces was <100 among the positive samples, and cases of heavy infection were limited, the endemicity of these pathogenic Eimeria species among farms in Indonesia should be noted.

DOI： 10.1016/j.parint.2019.101927

PubMed
インドネシア、ジャワ島の肉牛で認められた高病原性Eimeria種の分子的特徴(Molecular characterization of highly pathogenic Eimeria species among beef cattle on Java Island, Indonesia)

Ekawasti Fitrine, Nurcahyo Wisnu, Wardhana April Hari, Shibahara Tomoyuki, Tokoro Masaharu, Sasai Kazumi, Matsubayashi Makoto

Parasitology International 72 1 - 7 2019.10（ ISSN:1383-5769 ）

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ジャワ島のEimeria種とその他胃腸内寄生虫を調査し、その保有率を報告した。ジャワ島の農場の肉牛から糞便289検体を収集し、Whitlock法とショ糖液浮遊法によって調べた。陽性検体からショ糖液浮遊法によってEimeriaオーサイトを精製し、ゲノムDNAを抽出してPCRによってEimeria種の分子同定を行った。Eimeria種の保有率はWhitlock法では9.4%、ショ糖液浮遊法では52.3%であった。保有率は1歳未満が63.9%、1～2歳が75.0%、2歳以上が42.3%で、若年肉牛の方が保有率は高かった。同定した種の保有率はE.bovisが10.4%、E.ellipsoidalisが2.8%、E.alabamensisが2.1%、E.zuerniiが1.4%、E.auburnensisが1.1%、E.cylindricaが0.4%であった。Strongyloides papillosusの保有率が12.8%、Trichuris globulosaが7.3%、Capillaria bovisが0.3%であった。陽性検体中糞便1gあたりのオーサイト数は100未満で、重感染例は限られていたが、インドネシアの農場では病原性Eimeria種が流行していることが示された。
Susceptibility to various coccidiostats in the murine coccidian parasite Eimeria krijgsmanni

Kota Inoue, Masashi Tsujio, Makoto Matsubayashi, Ryota Inoue, Hitoshi Hatai, Masako Andoh, Keisuke Abe, Toshihiro Matsui, Tomohide Matsuo

Acta Parasitol. 2019.09

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Susceptibility to Various Coccidiostats in the Murine Coccidian Parasite Eimeria krijgsmanni. Reviewed

Kota Inoue, Masashi Tsujio, Makoto Matsubayashi, Ryota Inoue, Hitoshi Hatai, Masako Andoh, Keisuke Abe, Toshihiro Matsui, Tomohide Matsuo

Acta parasitologica 64 ( 2 ) 418 - 422 2019.06

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International / domestic magazine：International journal

INTRODUCTION: Murine Eimeria spp. have been used as effective experimental models of disease instead of large mammalian hosts such as cattle. We here examine drug susceptibility of the uncharacterized murine intestinal protozoan parasite, Eimeria krijgsmanni. MATERIALS AND METHODS: The effectiveness of different treatments against infection of E. krijgsmanni was examined for suppression of oocyst shedding: ST mixture ST mixture, pyrimethamine, Ektecin and toltrazuril. RESULTS: ST mixture and pyrimethamine did not suppress oocyst shedding effectively. Although therapeutic efficacy of Ektecin was demonstrated, the dose required was larger than that for cattle and chickens. Oocyst shedding was only completely suppressed completely by continuous administration of toltrazuril. Furthermore, it was confirmed through morphological examination that early developmental stage zoites appeared in host epithelial cells during and following treatment by toltrazuril, and toltrazuril could not eliminate residual zoites in epithelial cells. CONCLUSION: E. krijgsmanni may be relatively resistant to these anti-coccidian agents and might therefore have different characteristics that differ from other coccidia with regard to drug susceptibility.

DOI： 10.2478/s11686-019-00052-w

PubMed
Three monophyletic clusters in Retortamonas species isolated from vertebrates Reviewed

Hendarto J, Mizuno T, Hidayati APN, Rozi IE, Asih PBS, Syafruddin D, Yoshikawa H, Matsubayashi M, Tokoro M.

Parasitol Int. 69 93 - 98 2019.04

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Three monophyletic clusters in Retortamonas species isolated from vertebrates. Reviewed

Joko Hendarto, Tetsushi Mizuno, Anggi P N Hidayati, Ismail E Rozi, Puji B S Asih, Din Syafruddin, Hisao Yoshikawa, Makoto Matsubayashi, Masaharu Tokoro

Parasitology international 69 93 - 98 2019.04

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Retortamonas spp. has been reported as an intestinal parasite among various host organisms, including humans; however, its intra-genus molecular diversity has not yet been elucidated. Haplotypes of the 18S small subunit ribosomal RNA locus (1836-1899 bp) of Retortamonas spp. from humans (n = 8), pigs (n = 6), dogs (n = 1), goats (n = 16), water buffalos (n = 23), cattle (n = 7), rats (n = 3), and chickens (n = 5) were analyzed with references isolated from non-human mammals, amphibians, and insects. Phylogenetic and network analyses revealed a statistically supported three cluster formation among the vertebrate-isolated haplotypes, while insect-isolated haplotypes were independently clustered with Chilomastix. In the clade of vertebrate isolates, assemblage A (amphibian genotype), which included the amphibian references, was addressed as an out-group of the other clusters. Assemblage B (mammalian and chicken genotype) included most haplotypes from various mammals including humans with the haplotypes isolated from a chicken. Human isolates were all classified into this assemblage, thus assemblage B might correspond to R. intestinalis. Assemblage C (bovine genotype), which included specific haplotypes from water buffalos and cattle, was addressed as a sister lineage of assemblage B. Among the diversified haplotypes of assemblage B, a specific haplotype, which was identified from multiple host mammals (humans, dogs, pigs, cattle, water buffalos, elks, goats, and rats), indicates the potential zoonotic transmission of the Retortamonas among them. The genotyping classification of retortamonads could contribute to a better understanding of its molecular epidemiology, especially among humans and related host organisms.

DOI： 10.1016/j.parint.2018.12.004

PubMed
脊椎動物から単離されたRetortamonas種における3つの単系統クラスター(Three monophyletic clusters in Retortamonas species isolated from vertebrates)

Hendarto Joko, Mizuno Tetsushi, Hidayati Anggi P.N., Rozi Ismail E., Asih Puji B.S., Syafruddin Din, Yoshikawa Hisao, Matsubayashi Makoto, Tokoro Masaharu

Parasitology International 69 93 - 98 2019.04（ ISSN:1383-5769 ）

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2013～2015年にインドネシアのSumba島で採集したヒト(n=8)、ブタ(n=6)、イヌ(n=1)、ヤギ(n=16)、スイギュウ(n=23)、ウシ(n=7)、ラット(n=3)、ニワトリ(n=5)由来の糞便試料からRetortamonas種を単離し、18S rRNA遺伝子座(1836-1899bp)のハプロタイプを哺乳類、両生類、昆虫の既知データと比較分析した。系統発生解析およびネットワーク解析により、脊椎動物から単離されたハプロタイプに3つのクラスターが存在することが示され、昆虫から単離されたハプロタイプは独立したChilomastix spp.としてクラスター化された。脊椎動物のハプロタイプは両生類遺伝子型のA群、哺乳類・ニワトリ遺伝子型のB群、スイギュウ・ウシ遺伝子型のC群であり、B群はニワトリとヒトを含む様々な哺乳類由来の殆どのハプロタイプを含んでいた。ヒトの分離株は全てB群に分類されたのでB群はR.intestinalisに相当すると推測された。B群は多様な哺乳類でみられる特殊なハプロタイプであり、潜在的に人獣共通感染性のRetortamonasが示唆された。
Prevalence of micro-organisms associated with feline gingivostomatitis Reviewed

Nakanishi, Masaru Furuya, Takehisa Soma, Yoshiki Hayashiuchi, Ryusaku Yoshiuchi, Makoto Matsubayashi, Hiroyuki Tani, Kazumi Sasai

Journal of Feline Medicine and Surgery 21 ( 2 ) 103 - 108 2019.02

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Kind of work：Joint Work
国内初の遺伝子解析による豚鞭虫 (Trichuris suis) の同定

村松龍ノ助, 小沼成尚, 芝原友幸, 松林誠

秋田県獣医師会雑誌雑誌 118 14 - 17 2019.02

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Prevalence of microorganisms associated with feline gingivostomatitis. Reviewed

Hitoshi Nakanishi, Masaru Furuya, Takehisa Soma, Yoshiki Hayashiuchi, Ryusaku Yoshiuchi, Makoto Matsubayashi, Hiroyuki Tani, Kazumi Sasai

Journal of feline medicine and surgery 21 ( 2 ) 103 - 108 2019.02

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

OBJECTIVES: Feline gingivostomatitis (FGS) is a painful chronic inflammatory disease of the oral cavity. The purpose of this study was to examine the frequency of detection of certain common feline bacteria and viruses to determine any potential associations with FGS. METHODS: A multicentre case-control study design was conducted. In total, 72 control cats and 32 cats with FGS were included in the study. Oral swabs were cultured for bacterial identification and a PCR assay was carried out to examine the infection of feline calicivirus (FCV), feline herpesvirus-1 (FHV-1), Chlamydia felis, Mycoplasma felis and Bordetella bronchiseptica. RESULTS: There was a significant difference in age distribution between the control and the FGS group. Based on a PCR assay, the positive rate of FCV was significantly higher in FGS cats than control animals. For other infectious pathogens, including FHV-1, C felis and M felis, there was no significant difference. Bacterial culture of oral swabs revealed that Pasteurella multocida was most frequently detected, but the detection rate was significantly lower in FGS cats. In FGS cats, the incidence of Enterococcus faecalis and anaerobic bacteria were more frequently isolated than in control cats. CONCLUSIONS AND RELEVANCE: This study indicates that the positive rate of FCV was significantly higher in cats with FGS, and the microflora of the oral cavity of cats with FGS might be disrupted, although additional studies are required to compare the oral microbiome in cats of a variety of ages.

DOI： 10.1177/1098612X18761274

PubMed
Novel Characteristics of Mitochondrial Electron Transport Chain from Eimeria tenella. Reviewed

Makoto Matsubayashi, Daniel Ken Inaoka, Keisuke Komatsuya, Takeshi Hatta, Fumiya Kawahara, Kimitoshi Sakamoto, Kenji Hikosaka, Junya Yamagishi, Kazumi Sasai, Tomoo Shiba, Shigeharu Harada, Naotoshi Tsuji, Kiyoshi Kita

Genes 10 ( 1 ) 2019.01

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Eimeria tenella is an intracellular apicomplexan parasite, which infects cecal epithelial cells from chickens and causes hemorrhagic diarrhea and eventual death. We have previously reported the comparative RNA sequence analysis of the E. tenella sporozoite stage between virulent and precocious strains and showed that the expression of several genes involved in mitochondrial electron transport chain (ETC), such as type II NADH dehydrogenase (NDH-2), complex II (succinate:quinone oxidoreductase), malate:quinone oxidoreductase (MQO), and glycerol-3-phosphate dehydrogenase (G3PDH), were upregulated in virulent strain. To study E. tenella mitochondrial ETC in detail, we developed a reproducible method for preparation of mitochondria-rich fraction from sporozoites, which maintained high specific activities of dehydrogenases, such as NDH-2 followed by G3PDH, MQO, complex II, and dihydroorotate dehydrogenase (DHODH). Of particular importance, we showed that E. tenella sporozoite mitochondria possess an intrinsic ability to perform fumarate respiration (via complex II) in addition to the classical oxygen respiration (via complexes III and IV). Further analysis by high-resolution clear native electrophoresis, activity staining, and nano-liquid chromatography tandem-mass spectrometry (nano-LC-MS/MS) provided evidence of a mitochondrial complex II-III-IV supercomplex. Our analysis suggests that complex II from E. tenella has biochemical features distinct to known orthologues and is a potential target for the development of new anticoccidian drugs.

DOI： 10.3390/genes10010029

PubMed
Possible pathogenicity of commensal Entamoeba hartmanni revealed by molecular screening of healthy school children in Indonesia Reviewed

Takahiro Matsumura, Joko Hendarto, Tetsushi Mizuno, Din Syafruddin, Hisao Yoshikawa, Makoto Matsubayashi, Taro Nishimura, Masaharu Tokoro

Tropical Medicine and Health 2019.01
Novel characteristics of mitochondrial electron transport chain from Eimeria tenella

Makoto Matsubayashi, Daniel Ken Inaoka, Keisuke Komatsuya, Takeshi Hatta, Fumiya Kawahara, Kimitoshi Sakamoto, Kenji Hikosaka, Junya, Yamagishi, Kazumi Sasai, Tomoo Shiba, Shigaharu Harada, Naotoshi Tsuji, Kiyoshi Kita.

Genes 雑誌 10 ( 1 ) 2019.01

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Possible pathogenicity of commensal Entamoeba hartmanni revealed by molecular screening of healthy school children in Indonesia. Reviewed

Takahiro Matsumura, Joko Hendarto, Tetsushi Mizuno, Din Syafruddin, Hisao Yoshikawa, Makoto Matsubayashi, Taro Nishimura, Masaharu Tokoro

Tropical medicine and health 47 7 - 7 2019

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Publishing type：Research paper (scientific journal) International / domestic magazine：Domestic journal

Background: Although parasites are still endemic in developing areas, residents in those regions seem not to be affected by the presence of intestinal protozoans. This study aimed to investigate whether pathogenic and commensal protozoans are the causal agents of diarrhea via a school-based cross-sectional survey conducted in Indonesia, in September 2016. Results: Molecular screening for intestinal protozoans in collected 144 stool samples from healthy students (age range 7-15 years) was carried out. The prevalence of protozoan parasites was as follows: Giardia intestinalis (56.3%), Entamoeba histolytica (0%), E. dispar (6.9%), E. moshkovskii (0%), E. hartmanni (31.3%), and E. coli (44.4%). Observational evaluation of stool conditions using the Bristol stool chart confirmed the loose stool rate (33.3-90.9%) in each age group. Logistic regression analysis of protozoan infection or colonization for loose stool (mild to severe diarrhea) as an outcome revealed no significant findings in examined protozoans including pathogenic G. intestinalis infection [adjusted odds ratio (AOR) 0.78, 95% confidence interval (CI) 0.36-1.67], except in E. hartmanni colonization (AOR 2.81, 95% CI 1.1-3.7, P = 0.026). Conclusions: The molecular survey of intestinal protozoans targeting healthy population with their stool form evaluation could address the pathogenicity of those parasites appropriately. In comparatively higher-age children at least 7 years of age or greater in the endemic area, G. intestinalis could regard commensal, while E. hartmanni seems to possess a certain pathogenicity as a causal agent of mild diarrhea.

DOI： 10.1186/s41182-018-0132-7

PubMed
インドネシアの健康な学齢児を対象とした分子スクリーニングで明らかになった共生生物Entamoeba hartmanniの潜在的な病原性(Possible pathogenicity of commensal Entamoeba hartmanni revealed by molecular screening of healthy school children in Indonesia)

Matsumura Takahiro, Hendarto Joko, Mizuno Tetsushi, Syafruddin Din, Yoshikawa Hisao, Matsubayashi Makoto, Nishimura Taro, Tokoro Masaharu

Tropical Medicine and Health 47 1 of 5 - 5 of 5 2019（ ISSN:1348-8945 ）

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Giardia intestinalisとEntamoeba種の病原性を評価した。学校ベースで横断的検体採取を行った。健康な学齢児144名(男児74名、年齢7～15歳)から便を採取し、ブリストル便形状スケールにしたがって7段階のスコアに分類した後、便秘便(スコア1～2)、正常便(3～4)、軟便(5～7)に区分した。便からDNAを抽出し、G.intestinalis、Entamoeba種の18S rRNAを標的としたPCRを行った。各原虫の陽性率は、G.intestinalis(56.3%)、E.histolytica(0%)、E.dispar(6.9%)、E.moshkovskii(0%)、E.hartmanni(31.3%)、E.coli(44.4%)であった。各原虫陽性便の軟便率はG.intestinalis(66.7%)、E.dispar(80.0%)、E.hartmanni(68.9%)、E.coli(64.1%)であった。ロジスティック回帰分析により、軟便とE.hartmanni定着の間にのみ相関が認められた(AOR 2.81,95%CI:1.1～3.7,p=0.026)。流行地の7歳以上の小児においてはG.intestinalisは共生原虫とみなされると推測された。E.hartmanniはスコア5の便から検出されたことから、病原性は軽度なものに限られると考えられた。
Retrospective and histopathological studies of Entamoeba spp. and other pathogens associated with diarrhea and wasting in pigs in Aichi Prefecture, Japan Reviewed

Tetsuya Komatsu, Makoto Matsubayashi, Naoko Murakoshi, Kazumi Sasai, Tomoyuki Shibahara

Japan Agricultural Research Quarterly 53 ( 1 ) 59 - 67 2019（ ISSN:0021-3551 ）

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© 2019, Japan International Research Center for Agricultural Sciences. Postweaning diarrhea and wasting are a major concern in pig farms' management. Although hemolytic enterotoxigenic Escherichia coli, porcine epidemic diarrhea virus, porcine circovirus type 2, and Salmonella spp. are the most frequent etiological agents of these diseases, Entamoeba suis and E. polecki were recently reported to be associated with diarrhea in pigs. Since the infection rate of Entamoeba in pigs and its relationship with other pathogens are unknown, we examined 206 pigs exhibiting diarrhea and/or wasting in Aichi Prefecture, Japan to determine the prevalence of porcine Entamoeba spp. E. suis- and E. polecki-like trophozoites were detected by histopathology in 53 pigs, mainly in the lumen of the large intestine. Ulcerative colitis with infiltrating trophozoites was observed in 16 pigs, and most of these trophozoites were identified as E. polecki subtype 3 by PCR and sequence analysis. Tissue-invasive Entamoeba spp. were prevalent in pigs exhibiting diarrhea and wasting, and most samples were also positive for either Salmonella spp. or Lawsonia intracellularis by immunohistochemistry. These results suggested that Entamoeba was widespread in farms in Aichi Prefecture, and in most cases, enteritis was caused by coinfection by Entamoeba with Salmonella spp. or L. intracellularis, which causes wasting by exacerbating the original mucosal lesions.

DOI： 10.6090/jarq.53.59
First surveillance and molecular identification of the Cryptosporidium skunk genotype and Cryptosporidium parvum in wild raccoons (Procyon lotor) in Osaka, Japan

Hattori K, Donomoto T, Manchanayake T, Shibahara T, Sasai K, Matsubayashi M.

Parasitol Res 雑誌 2018.11

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Molecular identification of two Eimeria species, E. uekii and E. raichoi as type B, in wild Japanese rock ptarmigans, Lagopus muta japonica.

Makoto Matsubayashi, Sayaka Tsuchida, Atsushi Kobayashi, Tomoyuki Shibahara, Hiroshi Nakamura, Koichi Murata, Kazunari Ushida.

International Journal for Parasitology: Parasites and Wildlife 雑誌 2018.06

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Positive phototropism is accelerated in Biomphalaria glabrata snails by infection with Schistosoma mansoni Reviewed

Hiroki Maeda, Takeshi Hatta, Daigo Tsubokawa, Fusako Mikami, Toshiyuki Nishimaki, Takeshi Nakamura, Anisuzzaman, Makoto Matsubayashi, Motoyuki Ogawa, Clarissa Prazeres da Costa, Naotoshi Tsuji.

Parasitology International 雑誌 2018.06

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First detection and molecular identification of Entamoeba bovis from Japanese cattle. Parasitology Research, In press.

Makoto Matsubayashi, Yuu Matsuura, Satoko Nukata, Yuusuke Daizi, Tomoyuki Shibahara, Isao Teramoto, Tomohide Matsuo, Shigehiko Uni, Takeshi Hatta, Akira Kaneko, Naotoshi Tsuji, Kazumi Sasai

Parasitology Research 117 ( 1 ) 339 - 342 2018.01

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Retrospective and histopathological studies of Entamoeba spp. and other pathogens associated with diarrhea and wasting in pigs in Aichi Prefecture, Japan Reviewed

Tetsuya Komatsu, Makoto Matsubayashi, Naoko Murakoshi, Kazumi Sasai, Tomoyuki Shibahara.

Japan Agricultural Research Quarterly 2018
大分県におけるEimeria subsphericaによる牛の核内コクシジウム症.

河上友，松林誠，山田美那子，人見徹，笹井和美，芝原友幸.

日本獣医師会雑誌 2018

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鳥取県で確認されたEimeria subsphericaによる牛の核内コクシジウム症. Reviewed

岡田綾子、山里比呂志、今井直人、門田耕一、芝原友幸、松林　誠

牛臨床寄生虫研究会誌雑誌 2018

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Genetic and histopathological identification of Cystoisospora suis in a post-weaned piglet with watery diarrhea Reviewed

Kanamori K, Manchanayake T, Matsubayashi T, Imai N, Kobayashi Y, Sasai K, Shibahara T.

Japan Agricultural Research Quarterly 雑誌 52 ( 1 ) 55 - 61 2018
Surveillance of Eimeria species in wild Japanese rock ptarmigans, Lagopus muta japonica, and insight into parasitic seasonal life cycle at timberline regions of the Japanese Alps

Makoto Matsubayashi, Sayaka Tsuchida, Kazunari Ushida, Koichi Murata

International Journal for Parasitology: Parasites and Wildlife 2018

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Pathogenic characteristics of a novel intranuclear coccidia in Japanese black calves and its genetic identification as Eimeria subspherica Reviewed

Terunori Koreeda, Tomo Kawakami, Ayako Okada, Yoshimasa Hirashima, Naoto Imai, Kazumi Sasai, Shogo Tanaka, Makoto Matsubayashi, Tomoyuki Shibahara

Parasitology Research 雑誌 2017

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Genetic and histopathological identification of Cystoisospora suis in a post-weaned piglet with watery diarrhea

Kanamori K, Manchanayake T, Matsubayashi T, Imai N, Kobayashi Y, Sasai K, Shibahara T

Jarq-Japan Agricultural Research Quarterly 2017

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Zoonotic infection with Onchocerca dewittei japonica in an 11-year-old boy in Kansai Region, Western Honshu, Japan

Uni S, Fukuda M, Ogawa K, Lim YA, Agatsuma T, Bunchom N, Saijuntha W, Otsuka Y, Bhassu S, Udin ASM, Zainuri NA, Omar H, Nakatani J, Matsubayashi M, Maruyama H, Ramli R, Azirun MS, Takaoka H.

Parasitol. Int. 雑誌 2017

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ホルスタイン種成牛の消化管にみられたEimeria bovis の検出と形態学的観察

原陽子、東智子、山田学、松林誠

家畜診療雑誌 2017

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Serological surveillance for antibodies against six canine viruses in wild raccoons in Japan Reviewed

Emiko Aoki, Takehisa Soma, Mayumi Yokoyama, Makoto Matsubayashi, Kazumi Sasai

Journal of Wildlife Diseases 雑誌 2017

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5カ月齢の黒毛和種雌牛にみられた肝コクシジウム症 Reviewed

瀧澤光華、樋口明宏、松林誠、笹井和美、金平克史、芝原友幸

家畜診療雑誌 2017

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Development of molecular diagnostic protocols for detecting three types of Entamoeba from diarrheal and asymptomatic pigs and environmental moist soils. Reviewed

Yoshimasa Hirashima, Tilusha Manchanayake, Takahisa Yano, Syoei Kitahara, Terunori Koreeda, Syunsuke Kamimura, Kazumi Sasai, Makoto Matsubayashi, Tomoyuki Shibahara

Parasitology Research 2017

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Initial development of Babesia ovata in the tick midgut Reviewed

Maeda H, Hatta T, Alim MA, Tsubokawa D, Mikami F, Kusakisako K, Matsubayashi M, Umemiya-Shirafuji R, Tsuji N, Tanaka T.

Vet Parasitol. 雑誌 2017

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Morphological and molecular characteristics of Malayfilaria sofiani Uni, Mat Udin & Takaoka n. g., n. sp. (Nematoda: Filarioidea) from the common treeshrew Tupaia glis Diard & Duvaucel (Mammalia: Scandentia) in Peninsular Malaysia. Reviewed

Shigehiko Uni, Ahmad Syihan Mat Udin, Takeshi Agatsuma, Weerachai Saijuntha, Rosli Ramli, Yvonne Lim Ai Lian, S. Sivanandam, Emilie Lefoulon, Coralie Martin, Daicus M. Belabut, Saharul Kasim, Muhammad Rasul Abdullah Halim, Hasmahzaiti Omar, Subha Bhassu, Masako Fukuda, Makoto Matsubayashi, Masashi Harada, Chen Chee Dhang, Rosli Hashi, Hiroyuki Takaoka, Mohd Sofian Azirun.

Parasit Vectors 雑誌 2017

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Report of fatal mixed infection with Cryptosporidium parvum and Giardia intestinalis in neonatal calves Reviewed

Yuu Matsuura, Makoto Matsubayashi, Satoko Nukata, Tomoyuki Shibahara, Osamu Ayukawa, Yasuko Kondo, Tomohide Matsuo, Shigehiko Uni, Masaru Furuya, Hiroyuki Tani, Naotoshi Tsuji, Kazumi Sasai

Acta Parasitologica 2017

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Transcriptional profiles of virulent and precocious strains of Eimeria tenella at sporozoite stage; novel biological insight into attenuated asexual development Reviewed

Makoto Matsubayashi, Fumiya Kawahara, Takeshi Hatta, Junya Yamagishi, Takeharu Miyoshi, Anisuzzaman, Kazumi Sasai, Takashi Isobe, Kiyoshi Kita, Naotoshi Tsuji

Infection, Genetics and Evolution 雑誌 2016.02

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Course of induced infection by Eimeria krijgsmannni in immunocompetent and immunodeficient mice Reviewed

Yuina Ono, Makoto Matsubayashi, Hiroaki Kawaguchi, Masashi Tsujio, Masanobu Mizuno, Tetsuya Tanaka, Tatsunori Masatani, Toshihiro Matsui, Tomohide Matsuo

Acta Parasitol. 雑誌 115 ( 1 ) 211 - 5 2016.01

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A serological survey of antobodied against Leptospira spp. from wild raccoons (Procyon lotor) in Osaka, Japan Reviewed

Saeki Jun, Nakanishi Hitoshi, Masubuchi Katsuo, Matsubayashi Makoto, Furuya Masaru, Tani Hiroyuki, Sasai Kazumi

Asian Journal of Animal and Veterinary Advances 雑誌 2016.01

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Establishment of a novel tick-Babesia experimental infection model Reviewed

Maeda H, Hatta T, Alim MA, Tsubokawa D, Mikami F, Matsubayashi M, Miyoshi T, Umemiya-Shirafuji R, Kawazu SI, Igarashi I, Mochizuki M, Tsuji N, Tanaka T

Scientific Reports 雑誌 2016

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Detection of kobuvirus RNA in Japanese domestic dogs Reviewed

Takehisa Soma, Makoto Matsubayashi, Kazumi Sasai

Journal of Veterinary Medical Science 雑誌 2016

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Large-svale one-year survey of Trichinella spp. infection in wild boar (Sus scrofa) in Japan Reviewed

T. Hatta, K. Imamura, T. Yamamoto, M. Matsubayashi, N. Tsuji, T. Tsutsui

Japanese Journal of Infectious Diseases 雑誌 2016

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Identification of Eimeria acervulina conoid antigen using chicken monoclonal antibody Reviewed

Makoto Matsubayashi, Chisa Minoura, Shintaro Kimura, Hiroyuki Tani, Masaru Furuya, Hyun S. Lillehoj, Haruo Matsuda, Shigeo Takenaka, Takeshi Hatta, Naotoshi Tsuji, Kazumi Sasai

Parasitology Research 雑誌 2016

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豚のEntamoeba属原虫感染による潰瘍性大腸炎 Reviewed

矢口裕司，松林誠，板橋知子，藤井勇紀，小貫登輝夫，笹井和美，小林勝，芝原友幸

日本獣医師会雑誌雑誌 2016

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First report of molecular identification of Cystoisospora suis in a piglet with lethal diarrhea in Japan. Reviewed

Makoto Matsubayashi, Hideko Takayama, Masahiro Kusumoto, Misato Murata, Yuka Uchiyama, Masaya Kaji, Kazumi Sasai, Ryosaku Yamaguchi, Tomoyuki Shibahara

Acta Parasitologica 雑誌 61 ( 2 ) 2016

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First report of mixed Entamoeba polecki (ST 1) and E. suis infection in piglets shedding abnormalfeces by histopathological and molecular surveys Reviewed

Makoto Matsubayashi, Yuri Sasagawa, Tsunehiko Aita, Masaharu Tokoro,Makoto Haritani, Tomoyuki Shibahara

Acta Parasitologica 雑誌 2016

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DB-AT: a 2015 update to the Full-parasites database brings a multitude of new transcriptomic data for apicomplexan parasites Reviewed

Jąkalski M, Wakaguri H, Kischka TG, Nishikawa Y, Kawazu SI, Matsubayashi M, Kawahara F, Tsuji N, Cao S, Sunaga F, Xuan X, Okubo K, Igarashi I, Tuda J, Mongan AE, Eshita Y, Maeda R, Makałowski W, Suzuki Y, Yamagishi J.

Nucleic Acids Research 雑誌 43 ( Database issue ) D631 - 6 2015.01

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豚の消化器疾患におけるEntamoeba poleckiとEntamoeba suisの病原性 Reviewed

金森健太、貞弘真行、松林　誠、所　正治、阿部　仁一郎、播谷　亮、芝原友幸

日本豚病研究会報雑誌 2015

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離乳後問題となる疾病 Reviewed

芝原友幸、宮﨑綾子、生澤充隆、山口遼作、松林　誠.

日本豚病研究会報雑誌 66 22 - 25 2015

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ペルオキシダーゼによる退色反応の過酸化水素に対する耐性 Reviewed

森崎真奈美, 松林誠, 森田みゆき　　

繊維学会誌雑誌 2015

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Infective larvae of Cercopithifilaria spp. (Nematoda: Onchocercidae) from hard ticks (Ixodidae) recovered from the Japanese serow (Bovidae) Reviewed

Uni S., Bain O., Fujita H., Matsubayashi M., Fukuda M., Takaoka H.

Parasitology International 雑誌 2015

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パラフィン包埋切片を用いた種特異的PCRによる子牛の出血性直腸炎におけるコクシジウムの遺伝子学的同定 Reviewed

是枝輝紀，野尻麻里子，堂下さつき，松林　誠，芝原友幸，岡野良一

日本獣医師会雑誌雑誌 68 297 - 300 2015

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豚のアメーバ症；出血性大腸炎の豚から同定されたEntamoeba suis Reviewed

鈴田史子、松林　誠、寺山好美、下條憲吾、油井　武、播谷　亮、芝原友幸

日本豚病研究会報. 雑誌 65 44 - 50 2015

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First molecular identification of Entamoeba polecki in a piglet in Japan and implications for aggravation of ileitis by coinfection with Lawsonia intracellularis Reviewed

Matsubayashi M, Kanamori K, Sadahiro M, Tokoro M, Abe N, Haritani M, Shibahara T

Parasitology Research 雑誌 2015

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Evaluation of Eimeria krijgsmanni as a murine model for testing the efficacy of anti-parasitic agents. Reviewed

Takeo T, Tanaka T, Matsubayashi M, Tsujio M, Umemiya-Shirafuji R,Tsuji N, Fujisaki K, Matsui T, Matsuo T

Acta Parasitologica 雑誌 60 ( 2 ) 2015

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The Detection of toxigenic Corynebacterium ulcerans from cats with nasal inflammation symptom in Japan Reviewed

Saeki J, Katsukawa C, Matsubayashi M, Nakanishi N., Furuya M, Tani H, Sasai K.

Epidemiology and Infection. 雑誌 2015

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Localization of heat shock protein 110 in canine mammary gland tumor Reviewed

Okada S, Furuya M, Takenaka S, Fukui A, Matsubayashi M, Tani H, Sasai K

Veterinary Immunology and Immunopathology 雑誌 2015

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Genetic identification of Entamoeba polecki subtype 3 from pigs in Japan and characterisation of its pathogenic role in ulcerative colitis Reviewed

Matsubayashi M, Murakoshi N, Komatsu T, Tokoro M, Haritani M, Shibahara T

Infection, Genetics and Evolution 雑誌 2015

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Genetic analysis of Enterobius vermicularis isolated from a chimpanzee with lethal hemorrhagic colitis and pathology of the associated lesions Reviewed

Yaguchi Y, Sachi Okabayashi S, Abe N, Masatou H, Iida S, Teramoto I, Matsubayashi M, Shibahara T.

Parasitology Research 雑誌 113 ( 11 ) 4105 - 9 2014.11

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Longistatin secreted with tick saliva blocks RAGE Reviewed

Anisuzzaman, Hatta T, Miyoshi T, Matsubayashi M, Islam MK, Alim MA, Anas MA, Hasan MM, Matsumoto Y, Yamamoto Y, Yamamoto H, Fujisaki K, Tsuji N.

Journal of Clinical Investigation 雑誌 124 ( 10 ) 4429 - 44. 2014.10

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Ultrastructural characteristics and molecular identification of Entamoeba suis isolated from pigs with hemorrhagic colitis: implications for pathogenicity. Reviewed

Matsubayashi M, Suzuta F, Terayama Y, Shimojo K, Yui T, Haritani M, Shibahara T

Parasitology Research 雑誌 113 ( 8 ) 3023 - 8 2014.08

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Host specificity and in vivo infectivities of the mouse coccidian parasites Eimeria krijgsmanni Reviewed

Hashimoto K, Tanaka T, Matsubayashi M, Endo K, Umemiya-Shirafuji R, Matsui T, Matsuo T

Acta Parasitologica 雑誌 59 ( 2 ) 337 - 42. 2014.06

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Localization of eimeripain, an Eimeria tenella cathepsin B-like cysteine protease, during asexual and sexual intracellular development in chicken ceca Reviewed

Matsubayashi M, Hatta T, Miyoshi T, Anisuzzaman, Sasai K, Yamaji K, Shimura K, Isobe T, Kita K, Tsuji N

Journal of Veterinary Medical Science 雑誌 76 ( 4 ) 531 - 7. 2014.05

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Molecular and phylogenetic characterizations of an Eimeria krijgsmanni Yakimoff & Gouseff, 1938 (Apicomplexa: Eimeriidae) mouse intestinal protozoan parasite by partial 18S ribosomal RNA gene sequence analysis. Reviewed

Takeo T, Tanaka T, Matsubayashi M, Maeda H, Kusakisako K, Matsui T, Mochizuki M, Matsuo T

Parasitology International 雑誌 63 ( 4 ) 627 - 630. 2014.04

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Survey on gastrointestinal parasites and detection of Cryptosporidium spp. on cattle in West Java, Indonesia Reviewed

Ananta S M, Suharno, Hidayat A, Matsubayashi M

Asian Pacific Journal of Tropical Medicine 雑誌 7 ( 3 ) 197 - 201. 2014.03

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Age-related detection and molecular characterization of Cryptosporidium suis and Cryptosporidium scrofarum in pre-and post-weaned piglets and adult pigs in Japan. Reviewed

Yui T, Nakajima T, Yamamoto N, Kon M, Abe N, Matsubayashi M, Shibahara T

Parasitology Research 雑誌 113 ( 1 ) 359 - 65. 2014.01

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マイクロアレイを用いたフタトゲチマダニにおける殺ダニ剤応答遺伝子の探索 Reviewed

八田岳士、松林誠、辻尚利.

動物衛生研究所研究報告雑誌 120 69 - 72 2014

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鶏コクシジウムの新規薬剤開発を目指した盲腸内発育期の環境適応代謝経路に関する研究 Reviewed

松林誠、八田岳士、辻尚利

動物衛生研究所研究報告雑誌 120 61 - 64 2014

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Elongation Factor-1α is a Novel Protein Associated with Host Cell Invasion and a Potential Protective Antigen of Cryptosporidium parvum. Reviewed

Matsubayashi M, Teramoto-Kimata I, Uni S, Lillehoj H S, Matsuda H, Furuya M, Tani H, Sasai K

Journal of Biological Chemistry. 雑誌 288 ( 47 ) 34111 - 20 2013.11

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High-throughput RNA sequencing profiles and transcriptional evidence of aerobic respiratory enzymes in sporulating oocysts and sporozoites of Eimeria tenella. Infection, Genetics and Evolution Reviewed

Matsubayashi M, Hatta T, Miyoshi T, Anisuzzaman, Sasai K, Shimura K, Isobe T, Kita K, Tsuji N

Infection, Genetics and Evolution 雑誌 18 269 - 76. 2013.08

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HlCPL-A, a cathepsinL-like cysteine protease from the ixodid tick Haemaphysalis longicornis, modulated midgut proteolytic enzymes and their inhibitors during blood meal digestion Reviewed

Yamaji K, Miyoshi T, Hatta T, Matsubayashi M, Abdul Alim M, Anisuzzaman, Kushibiki S, Fujisaki K, Tsuji N.

Infection, Genetics and Evolution 雑誌 16 206 - 11. 2013.06

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Infectivity of Cryptosporidium andersoni Kawatabi type relative to the small number of oocysts in immunodeficient and immunocompetent neonatal and adult mice. Reviewed

Nagano-Koyashiki S, Matsubayashi M, Kimata I, Furuya M, Tani H, Sasai K.

Parasitology International 雑誌 62 ( 2 ) 109 - 111. 2013.04

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Quantitative PCR-based parasite burden estimation of Babesia gibsoni in the vector tick, Haemaphysalis longicornis (Acari: Ixodidae), fed on an experimentally infected dog Reviewed

Hatta T, Matsubayashi M, Miyoshi T, Islam M K, Alim M A, Anisuzzaman,Yamaji K, Fujisaki K, Tsuji N

Journal of Veterinary Medical Science 雑誌 75 ( 1 ) 1 - 6. 2013.01

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Infective larvae of Cercopithifilaria spp. (Nematoda: Onchocercidae) from hard ticks (Ixodidae) recovered from the Japanese serow (Bovidae) Reviewed

Uni S, Bain O, Fujita H, Matsubayashi M, Fukuda M, Takaoka H

Parasite 雑誌 20 ( 1 ) 1 - 7 2013.01

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Semi-artificial mouse　skin membrane feeding technique for adult tick, Haemaphysalis longicornis. Reviewed

Hatta T, Miyoshi T, Matsubayashi M, Islam MK, Alim MA, Anisuzzaman, Yamaji K, Fujisaki K, Tsuji N.

Parasites & Vectors 雑誌 5 ( 263 ) doi: 10.1186/1756 - 3305 2012.12

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A hemocyte-derived Kunitz-BPTI-type chymotrypsin inhibitor, HlChI, from the ixodid tick Haemaphysalis longicornis, plays regulatory functions in tick blood-feeding processes. Reviewed

Alim MA, Islam MK, Anisuzzaman, Miyoshi T, Hatta T, Yamaji K, Matsubayashi M, Fujisaki K, Tsuji N

Insect Biochemistry and Molecular Biology 雑誌 42 ( 12 ) 925 - 34. 2012.12

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Synchronous development of Eimeria tenella in chicken ceca and utility of laser microdissection for purification of single stage schizont RNA. Reviewed

Matsubayashi M, Hatta T, Miyoshi T, Anisuzzaman, Alim M A, Yamaji, K. Shimura K. Isobe T, Tsuji N

Parasitology 雑誌 139 ( 12 ) 1553 - 61 2012.10

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Phylogenetic identification of Cystoisospora spp. from dogs, cats, and raccoon dogs in Japan Reviewed

Matsubayashi M, Carreno RA, Tani H, Yoshiuchi R, Kanai T, Kimata I, Uni S, Furuya M, Sasai K

Veterinary Parasitology 雑誌 176 ( 2-3 ) 270 - 4 2011.03

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Effect of low pH on the morphology and viability of Cryptosporidium andersoni sporozoites and histopathology in the stomach of infected mice Reviewed

Matsubayashi M, Ando H, Kimata I, Takase H, Nakagawa H, Furuya M, Tani T, Sasai K

International of Journal for Parasitology 雑誌 41 ( 3-4 ) 287 - 92 2011.03

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Survey and molecular characterization of Cryptosporidium and Giardia spp. in owned companion animals, dogs and cats, in Japan Reviewed

Yoshiuchi R, Matsubayashi M, Kimata I, Furuya M, Tani H, Sasai K

Veterinary Parasitology 雑誌 174 ( 3-4 ) 313 - 6. 2010.12

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Kind of work：Joint Work
Morphological changes and viability of Cryptosporidium parvum sporozoites after excystation in cell-free culture media Reviewed

Matsubayashi M, Ando H, Kimata I, Nakagawa H, Furuya M, Tani H, Sasai K

Parasitology 雑誌 137 ( 13 ) 1861 - 6. 2010.11

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Kind of work：Joint Work
Coprological survey of parasitic infections in pigs and cattle in slaughterhouse in Osaka, Japan. Reviewed

Matsubayashi M, Kita T, Narushima T, Kimata I, Tani H, Sasai K, Baba E

Journal of Veterinary Medical Science. 雑誌 71 ( 8 ) 1079 - 83 2009.08

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Kind of work：Joint Work
Genetical survey of novel type of Cryptosporidium andersoni in cattle in Japan. Reviewed

Matsubayashi M, Nagano S, Kita T, Narushima T, Kimata I., Iseki M, Hajiri T, Tani H, Sasai K, Baba E

Veterinary Parasitology. 雑誌 158 ( 1-2 ) 44 - 50 2008.12

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Kind of work：Joint Work
Characterization of monoclonal antibodies that recognize the Eimeria tenella microneme protein MIC2 Reviewed

Sasai K, Fetterer R H, Lillehoj H, Matusra S, Constantinoiu C C, Matsubayashi M, Tani H, Baba E

Journal of Parasitology 雑誌 94 ( 6 ) 1432 - 4 2008.11

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Kind of work：Joint Work
Electron microscopic observation of cytoskeletal frame structures and detection of tubulin on the apical region of Cryptosporidium parvum sporozoites Reviewed

Matsubayashi M, Takase H, Kimata I, Nakagawa H, Tani H, Sasai K, Baba E

Parasitology 雑誌 135 ( Pt 3 ) 295 - 301 2008.03

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Kind of work：Joint Work
Detection of a mixed infection of a novel Cryptosporidium andersoni and its subgenotype in Japanese cattle Reviewed

Nagano S., Matsubayashi M., Kita T., Narushima T., Kimata I., Iseki M., Hajiri T., Tani H., Sasai K., Baba E

Veterinary Parasitology. 雑誌 149 ( 3-4 ) 213 - 218. 2007.11

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Kind of work：Joint Work
Monoclonal antibodies for the diagnosis of canine mastocytoma. Reviewed

Sasai K, Katoh M, Fujita D, Yamashita M, Constantinoiu C C, Matsubayashi M, Tani H, Baba E

Hybridoma (Larchmt). 雑誌 26 ( 3 ) 162 - 7 2007.06

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Kind of work：Joint Work
短期大学女子学生の安静時代謝量と運動状況について Reviewed

松林　誠, 西端咲子, 中西輝雄

大阪夕陽丘学園短期大学紀要雑誌 51 13 - 20 2007

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Kind of work：Joint Work
国内の動物園で飼育されていたシシオザルにおけるイソスポラ属原虫の検出 Reviewed

松林　誠、高見一利、木俣　勲、谷　浩行、笹井和美、馬場栄一郎

動物園水族館雑誌. 雑誌 48 ( 1 ) 9 - 13 2006.12

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Kind of work：Joint Work
Subgenotype analysis of Cryptosporidium parvum isolates from humans and animals in Japan using the 60-kDa glycoprotein gene sequences. Reviewed

Abe N, Matsubayashi M, Kimata I, Iseki M

Parasitology Research 雑誌 99 ( 3 ) 303 - 5 2006.08

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Kind of work：Joint Work
女子短大生の過去10年間の身体状況について Reviewed

松林　誠,　朴　商夏,　中西輝雄

大阪夕陽丘学園短期大学紀要雑誌 50 1 - 6 2006

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Kind of work：Joint Work
Molecular characterization of crane Coccidia, Eimeria gruis and E. reichenowi, found in feces of migratory cranes Reviewed

Matsubayashi M, Takami K, Abe N, Kimata I, Tani T, Sasai K., Baba E

Parasitology Research 雑誌 97 ( 1 ) 80 - 3 2005.08

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Kind of work：Joint Work
Survey of Cryptosporidium spp. and Giardia spp. infections in various animals at a zoo in Japan. Reviewed

Matsubayashi M, Takami K, Kimata I, Nakanishi T, Tani H, Sasai K, Baba E

Journal of Zoo and Wildlife Medicine 雑誌 36 ( 2 ) 331 - 5 2005.06

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Kind of work：Joint Work
Identification of Giardia intestinalis isolates from a human and cattle in Japan Reviewed

Matsubayashi M, Kimata I, Abe N

Journal of Veterinary Medical Science 雑誌 67 ( 3 ) 337 - 40. 2005.04

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Kind of work：Joint Work
Infectivity of a novel type of Cryptosporidium andersoni to laboratory mice Reviewed

Matsubayashi M, Kimata I, Iseki M, Hajiri T, Tani T, Sasai K, Baba E

Veterinary Parasitology 雑誌 129 ( 1-2 ) 165 - 8 2005.04

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Kind of work：Joint Work
Cross-reactivities with Cryptosporidium spp. by chicken monoclonal antibodies that recognize avian Eimeria spp. Reviewed

Matsubayashi M, Kimata I, Iseki M, Lillehoj H S, Matsuda H, Nakanishi T, Tani H, Sasai K, Baba E

Veterinary Parasitology 雑誌 128 ( 1-2 ) 47 - 57 2005.03

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Kind of work：Joint Work
The detection of a novel type of Cryptosporidium andersoni oocyst in cattle in Japan Reviewed

Matsubayashi M, Kimata I, Abe N, Tani H, Sasai K

Parasitology Research. 雑誌 93 ( 6 ) 504 - 506 2004.08

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Kind of work：Joint Work
飼い犬におけるGiardia とCryptosporidium の混合感染 Reviewed

松林　誠，阿部仁一郎，木俣　勲，竹澤泰子，谷浩行，笹井和美，馬場栄一郎

日本獣医師会雑誌雑誌 57 317 - 320 2004.05

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Kind of work：Joint Work
Characterization of stage-specific and cross-reactive antigens from Eimeria acervulina by chicken monoclonal antibodies Reviewed

Constantinoiu C. C., Lillehoj H.S., Matsubayashi M., Tani H., Matsuda H., Sasai K., Baba E.

Journal of Veterinary Medical Science 雑誌 66 ( 4 ) 403 - 408 2004.04

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Kind of work：Joint Work
First record of Cryptosporidium infection in a raccoon dog (Nyctereutes Procyonoides Viverrinus) Reviewed

Matsubayashi M, Abe N, Takami K, Kimata I, Iseki M., Nakanishi T, Tani H, Sasai K, Baba E

Veterinary Parasitology 雑誌 120 ( 3 ) 171 - 175 2004.03

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Kind of work：Joint Work
食品衛生における水系原虫感染症について Reviewed

松林　誠，中西輝雄，笹井和美

大阪女子学園短期大学紀要雑誌 48 1 - 8 2004

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Kind of work：Joint Work
Analysis of cross-reactivity of five new chicken monoclonal antibodies which recognize the apical complex of Eimeria using confocal laser immunofluorescence assay Reviewed

Constantinoiu C C, Lillehoj H S, Matsubayashi M, Hosoda Y, Tani H, Matsuda H, Sasai K, Baba E

Veterinary Parasitology 雑誌 118 ( 1-2 ) 29 - 35 2003.12

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Kind of work：Joint Work
Characteristic of a Hepatozoonosis in Lungs of Japanese Black Bears (Ursus thibetanus japonicus) Reviewed

Uni S, Matsubayashi M, Ikeda E, Suzuki Y

Journal of Veterinary Medical Science 雑誌 65 ( 3 ) 385 - 388 2003.03

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Kind of work：Joint Work
First Description of Isospora spp. from the Banded Mongoose, Mungos mungo, (Herpstidae) in Japan Reviewed

Matsubayashi M, Takami K, Nakanishi T, Sasai K

Bulletin of Osaka Joshigakuen Junior College 雑誌 45 43 - 48 2001

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Kind of work：Joint Work
CRIPTOSPORIDIOSIS -Review and Current Topics in Authors’ Research- Reviewed

Matsubayashi M, Nakanishi T, Sasai K

Bulletin of Osaka Joshigakuen Junior College 雑誌 44 43 - 50 2000

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Books and Other Publications

新しい子牛の科学 -胎生期から初産分娩まで-

家畜感染症学会編 (松林誠分担執筆)　

緑書房 2021.03
家禽疾病学 (獣医学教育モデル・コア・カリキュラム(2019年度版))準拠

分担執筆；松林　誠

鶏病研究会 2021.03
家禽疾病学 (獣医学教育モデル・コア・カリキュラム(2019年度版))準拠

分担執筆, 松林誠（ Role： Contributor）

鶏病研究会 2021.03 （ ISBN:9784990812003 ）

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Total pages：292p

CiNii Books
新しい子牛の科学 -胎生期から初産分娩まで-

家畜感染症学会編 (松林誠分担執筆)（ Role： Joint author , 3-1. 寄生虫感染症）

緑書房 2021.03
神の鳥ライチョウの生態と保全 -日本の宝を未来につなぐ-

楠田哲士, 秋葉由紀, 浅川満彦, 牛田一成, 松林誠他 (担当:分担執筆)（ Role： Joint author）

緑書房 2020.12
神の鳥ライチョウの生態と保全-日本の宝を未来につなぐ-

楠田哲士, 秋葉由紀, 浅川満彦, 牛田一成, 松林誠他（ Role： Contributor）

緑書房 2020.12
寄生虫のはなし

永宗喜三郎, 常盤俊大, 島野智之, 松林　誠 (担当:分担執筆)

朝倉書店 2020.10
寄生虫のはなし-この素晴らしき、虫だらけの世界-

永宗喜三郎, 常盤俊大, 島野智之編, 松林誠他（ Role： Contributor）

朝倉書店 2020.10
寄生虫病学 (獣医学教育モデル・コア・カリキュラム)

筏井宏美, 池和憲, 松尾智英, 松林誠他

緑書房 2020.09
寄生虫病学 (獣医学教育モデル・コア・カリキュラム)

筏井宏美, 池和憲, 松尾智英, 松林誠他編集

緑書房 2020.09
動物病院スタッフのための犬と猫の感染症ガイド : 適切でわかりやすいインフォームのために

小沼, 守, 前田, 健, 佐藤, 宏, 松林誠（ Role： Contributor）

緑書房 2019.11 （ ISBN:9784895313681 ）

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Total pages：166p

CiNii Books
動物寄生虫病学

藤崎幸蔵、板垣匡、松林誠他 (分担)（ Role： Joint author）

朝倉書店 2019.04
飼い主のための犬と猫の感染症診療

前田健、佐藤宏(編)、松林誠他（ Role： Joint author）

緑書房 2019
動物の感染症〈第四版〉

明石博臣、大橋和彦、松林誠他（ Role： Joint author）

近大出版 2019
動物寄生虫病学

板垣, 匡, 藤崎, 幸蔵, 筏井, 宏実, 井上, 昇, 奥, 祐三郎, 工藤, 上, 佐藤, 宏, 菅沼, 啓輔, 杉山, 広, 平, 健介, 田仲, 哲也, 野中, 成晃, 福本, 真一郎, 松林, 誠, 松本, 淳, 横山, 直明（ Role： Contributor）

朝倉書店 2019 （ ISBN:9784254460377 ）

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Total pages：v, 359p

CiNii Books
動物の感染症 (第四版)

明石, 博臣, 内田, 郁夫, 大橋, 和彦, 後藤, 義孝, 須永, 藤子, 高井, 伸二, 宝達, 勉, 松林誠（ Role： Contributor）

近代出版 2019 （ ISBN:9784874022504 ）

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Total pages：xvi, 32, 301p

CiNii Books
動物寄生虫病学

板垣匡, 藤崎幸蔵, 筏井宏実, 井上昇, 奥祐三郎 , 工藤上, 佐藤宏 , 菅沼啓輔, 杉山広, 平健介, 田仲哲也, 野中成晃 , 福本真一郎, 松林誠, 松本淳, 横山直明

朝倉書店 2019 （ ISBN:9784254460377 ）

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CiNii Books
動物病院スタッフのための犬と猫の感染症ガイド.

前田健、佐藤宏(編)、松林誠他（ Role： Joint author）

緑書房 2018
鶏病検査マニュアル

松林　誠 (分担執筆) （ Role： Joint author）

鶏病研究会 2016.11
獣医寄生虫学実習ハンドブック(第3版)

大西義博、松林誠.

三恵社 2015
「Eimeria」 (細菌・真菌・ウィルス便覧)

松林誠（ Role： Joint author）

技術情報協会 2014.03

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Responsible for pages：372-375
本邦で検出されCryptosporidium andersoni novel type について. Recent Advances in Medical Science: Parasites and their Human and Animal Hosts （医科学の進歩：寄生虫とその宿主たるヒトと動物）

松林　誠、古屋敷(長野)早希、木俣　勲、木太俊雅、古家　優、谷　浩行、笹井和美（ Role： Joint author）

第65回日本寄生虫学会西日本支部大会事務局 2010.10

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Responsible for pages：57-60

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MISC

牛の核内コクシジウム症

是枝輝紀, 猜都勇介, 松林誠, 芝原友幸

日本獣医師会雑誌 76 ( 2 ) e37 - e44 2023.02（ ISSN:0446-6454 ）
一酪農場におけるクリプトスポリジウム症の清浄化への試み Reviewed

高橋和瑛, 松尾智英, 山岸潤也, 芝原友幸, 笹井和美, 松林誠

家畜診療 715 47 - 48 2023.01
岡山県およびその近郊に生息する外来種を含む野生動物の寄生虫保有調査 Reviewed

森悠芽, 仲敦史, 池本茂豊, 阪田睦子, 林花奈子, 花島里奈, 太田啓介, 幸田良介, 石塚譲, 日根野谷淳, 芝原友幸, 笹井和美, 松林誠

岡山県自然保護センター研究報告 2023

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Publishing type：Rapid communication, short report, research note, etc. (bulletin of university, research institution)
高知県内で保護された野生鳥類の糞便検体による消化管寄生虫保有調査. Reviewed

井上春奈, 森悠芽, 畑中律敏, 芝原友幸, 笹井和美, 松林誠

日本野生動物医学会誌 2023
致死的下痢症の集団発生酪農場における複数種のクリプトスポリジウムの分子同定及びその感染動態と清浄化対策 Reviewed

河合愛実, 林みち子, 髙橋和瑛, 芝原友幸, 笹井和美, 松林誠

日本獣医師会雑誌 76 ( 2 ) 2023
鶏卵および鶏肉を原因とする食中毒とその対策

佐々木貴正, 岩田剛敏, 岡村雅史, 金山俊作, 花谷有樹子, 松林誠, 矢口和彦, 横山卓矢

鶏病研究会報 58 ( 3 ) 135 - 145 2022.11
脂肪肝出血症候群の現状と対策 Reviewed

須藤庸子,岩田剛敏,松林誠,花谷有樹子,木戸口勝彰,富田啓介

鶏病研究会報 58 ( 2 ) 65 - 70 2022.08

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【正確な検査結果のための現場におけるサンプリング】糞便検査のサンプリング　寄生虫編

松林誠

臨床獣医 40 ( 4 ) 21 - 25 2022.04（ ISSN:0912-1501 ）
正確な検査結果のための現場におけるサンプリング -糞便検査のサンプリング：寄生虫編-

松林誠

臨床獣医 40 ( 4 ) 21 - 25 2022.04
一酪農場におけるクリプトスポリジウム症清浄化への試み

高橋和瑛, 高橋和瑛, 松尾智英, 山岸潤也, 芝原友幸, 芝原友幸, 笹井和美, 松林誠

日本産業動物獣医学会(近畿)・日本小動物獣医学会(近畿)・日本獣医公衆衛生学会(近畿) 2022 2022

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J-GLOBAL
キジ科に寄生するEimeria 属原虫の形態学的特徴とその比較

木下萌美, 芝原友幸, 笹井和美, 松林誠

鶏病研究会報 56 ( 4 ) 174 - 178 2021.02

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鶏の免疫にかかわる最近の研究進展. Reviewed

杉山美樹, 松林誠, 永野哲司, 落合絢子, 岡村雅史, 斉藤恵子, 笛吹達史

鶏病研究会報 56 ( 4 ) 139 - 152 2021.02

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コクシジウムが関与した子牛の慢性壊死性腸炎 Reviewed

杉江建之介, 小松徹也, 川本尚未, 橋本幸江, 松林誠, 芝原友幸

家畜診療 2021
フタトゲチマダニ伸長因子1α(EF1α)遺伝子の上流領域解析(Promoter analysis of elongation factor 1-alpha(EF1α) derived from Haemaphysalis longicornis tick)

エマニュエル・ヘルナンデス, コフィ・コフィ, 坪川大悟, 三上房子, 松林誠, 辻尚利, 田仲哲也, 八田岳士

衛生動物 71 51 - 52 2020.03
Genetic identification of Tritrichomonas foetus using paraffin embedded sections of small and large intestines, and characterization of mucosal invasion in piglets

Yusuke ABETO, Naohide KITAHARA, Wakako MISUMI, Hirotaka ITO, Makoto MATSUBAYASHI, Kazumi SASAI, Tomoyuki SHIBAHARA

日本獣医師会雑誌 = Journal of the Japan Veterinary Medical Association 73 2020
わが国における鶏のロイコチトゾーン症と鶏マラリアの現状

松林誠, 井関博, 伊藤裕和, 岩田剛敏, 森腰俊亨, 中村賢司

鶏病研究会報 55 ( 3 ) 91 - 99 2019

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Authorship：Lead author Publishing type：Article, review, commentary, editorial, etc. (scientific journal)
国内初の遺伝子解析による豚鞭虫(Trichuris suis)の同定 (平成30年度獣医学術東北地区学会) -- (日本産業動物獣医学会(東北地区))

村松龍ノ助, 小沼成尚, 芝原友幸, 松林誠

秋田県獣医師会雑誌 = The journal of the Akitaken Veterinary Medical Association ( 118 ) 14 - 17 2019

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Presentations

日本国内のヌートリアから検出されたEimeria属原虫の形態観察および分子系統解析

大内宙, 仲敦史, 幸田良介, 石塚譲, 日根野谷淳, 芝原友幸, 笹井和美, 松林誠

日本野生動物医学会誌 2023.12 日本野生動物医学会
ニホンライチョウに寄生するEimeria属原虫の長期的感染動態

政兼菜実, 松林誠, 原藤芽衣, 山上達彦, 田村直也, 土田さやか, 小林篤, 芝原友幸, 中村浩志, 笹井和美, 牛田一成

日本野生動物医学会誌 2023.12 日本野生動物医学会
一酪農場におけるクリプトスポリジウム症の清浄化への試み

高橋和瑛, 松尾智英, 山岸潤也, 芝原友幸, 笹井和美, 松林誠

家畜診療 2023.01 (公社)全国農業共済協会
ウサギのEimeria stiedaeによる肝コクシジウム症

勝井一恵, 松林誠, 芝原友幸

日本獣医学会学術集会講演要旨集 2022.09 (公社)日本獣医学会
牛の新しい核内コクシジウムEimeria subsphericaの病原性と遺伝子学的同定法

是枝輝紀, 猜都勇介, 松林誠, 芝原友幸

日本獣医学会学術集会講演要旨集 2022.09 (公社)日本獣医学会
家禽や野生鳥類のアイメリア原虫とその病原性

松林誠, 芝原友幸

日本獣医学会学術集会講演要旨集 2022.09 (公社)日本獣医学会
国内の種鶏場におけるEimeria属原虫の浸潤状況調査

松林誠, 芝原友幸, 松尾智英, 畑生俊光, 山岸潤也, 笹井和美, 磯部尚

日本獣医学会学術集会講演要旨集 2021.09 (公社)日本獣医学会
Haemaphysalis longicornisに由来するelongation factor I-α(EFIα)のプロモータ分析(Promoter analysis of elongation factor I-alpha(EFI α) derived from Haemaphysalis longicornis)

Hernandez Emmanuel Pacia, 川田逸人, 山本大介, 加藤大智, 松林誠, 田仲哲也, 辻尚利, 八田岳士

日本寄生虫学会・日本臨床寄生虫学会合同大会プログラム・抄録集 2021.04 日本寄生虫学会・日本臨床寄生虫学会
臨床症例から形態的には漠然と診断されたDiphyllobothrim tapewormの検体の確証的な遺伝学的検査を用いた検証(Validation of morphologically ambiguously diagnosed Diphyllobothrium tapeworm samples in clinical cases using confirmatory genetic identification)

Ladzekpo Danielle, Ohari Yuma, Kwofie Kofi Dadzie, Alim Md Abdul, Kawada Hayato, Dadzie Samuel, Matsubayashi Makoto, Iwanaga Shiroh, Tsuji Naotoshi, Hatta Takeshi

日本寄生虫学会・日本臨床寄生虫学会合同大会プログラム・抄録集 2021.04 日本寄生虫学会・日本臨床寄生虫学会
日本における鶏アイメリアからの原虫共生ウイルスの網羅的検出と同定

村越ふみ, 田中悠太郎, 千葉悠斗, 浦山俊一, 萩原大祐, 松林誠, 中屋隆明

日本寄生虫学会・日本臨床寄生虫学会合同大会プログラム・抄録集 2021.04 日本寄生虫学会・日本臨床寄生虫学会
古典的なトランスクリプトーム分析に基づいた単為生殖性マダニのHaemaphysalis longicornisの胚形成に関する洞察(Insight into the embryogenesis of the parthenogenetic ixodid tick, Haemaphysalis longicornis based on a classic transcriptomic study)

Kwofie Kofi Dadzie, Hernandez Emmanuel Pacia, Kawada Hayato, Dadzie Samuel, Ladzekpo Danielle, Matsubayashi Makoto, Yamamoto Daisuke S., Kato Hirotomo, Iwanaga Shiroh, Tsuji Naotoshi, Hatta Takeshi

日本寄生虫学会・日本臨床寄生虫学会合同大会プログラム・抄録集 2021.04 日本寄生虫学会・日本臨床寄生虫学会
フタトゲチマダニの胚発生における古典的トランスクリプトーム解析(Classic transcriptomic study on embryonated eggs of the parthenogenetic ixodid tick, Haemaphysalis longicornis)

コフィ・コフィ , エマニュエル・ヘルナンデス , サミュエル・ダジ , ダニエル・ラゼクポ , 坪川大悟, 三上房子, 松林誠, 岩永史朗, 辻尚利, 八田岳士

衛生動物 2020.03 日本衛生動物学会

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Charge of on-campus class subject

獣医国際防疫学

2021
Special Lecture: Bioenvironmental Sciences C

2021
Veterinary Parasitology

2021
Veterinary Immunology

2021
Infectious Diseases of Animals C

2021
Basic clinical practices

2021 Practical Training
Global Circulation of Food and Hazards in Environments

2021
Practice in Veterinary Parasitology

2021 Practical Training
Veterinary International Prevention of Epidemics

2021
Special Lecture: Bioenvironmental Sciences C

2021
Special Study: Veterinary Environmental Sciences H

2021 Practical Training
Special Study: Veterinary Environmental Sciences G

2021 Practical Training
Special Study: Veterinary Environmental Sciences F

2021 Practical Training
Special Study: Veterinary Environmental Sciences E

2021 Practical Training
Special Study: Veterinary Environmental Sciences D

2021 Practical Training
Special Seminar: Veterinary Environmental Sciences C

2021
Special Seminar: Veterinary Environmental Sciences B

2021
Special Seminar: Veterinary Environmental Sciences A

2021
Veterinary Parasitology

2021
Veterinary Immunology

2021
Infectious Diseases of Animals C

2021
Practice in Veterinary Parasitology

2021 Practical Training
食品循環科学

2021

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Job title

Job title within the department

School of Veterinary Science Department of Veterinary Science

学科長 2023.04
Job title within the department

Graduate School of Veterinary Science Department of Veterinary Science

専攻長 2023.04 - 2024.03