Researcher Profiles - KOBAYASHI Koichi

写真a

KOBAYASHI Koichi

Organization

Faculty of Liberal Arts, Sciences and Global Education Professor
Graduate School of Science Department of Biological Chemistry
Graduate School of Science Department of Biological Chemistry

Affiliation

Institute of Liberal Arts, Sciences and Global Education

Position

Faculty of Liberal Arts, Sciences and Global Education

Professor 2025.04 - Now
Faculty of Liberal Arts, Sciences and Global Education

Associate Professor 2022.04 - 2025.03
Graduate School of Science Department of Biological Chemistry

Professor 2025.04 - Now
Graduate School of Science Department of Biology

Associate Professor 2022.07 - Now

Degree

博士（理学）（ Tokyo Institute of Technology ）

Research Areas

Life Science / Plant molecular biology and physiology / Plant Science
Life Science / Plant molecular biology and physiology

Research Interests

chloroplast
lipid
plant
photosynthesis
chlorophyll

Research subject summary

植物の葉緑体の発達の仕組みとその制御機構

Research Career

Regulatory mechanism of chloroplast development

Chloroplast, Plastid, Plant, Arabidopsis thaliana, Photosynthesis Individual

2018 - 2023
Regulation of chlorophyll metabolism

Chlorophyll, Tetrapyrrole Individual

2018 - 2023
Role of membrane lipids in chloroplast

Phospholipid, Glycolipid, Thylakoid membrane Individual

2018 - 2023

Professional Memberships

日本光合成学会

2009.05 - Now Domestic
日本植物学会

2009.01 - Now Domestic
日本植物生理学会

2008.01 - Now Domestic
日本植物脂質研究会

2007.04 - Now Domestic

Committee Memberships (off-campus)

Editor Journal of Plant Research

2025.01 - 2028.01

Awards

文部科学大臣表彰若手科学者賞

2015.04 文部科学省

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Country：Japan
日本植物学会奨励賞

2014.09 日本植物学会

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Country：Japan

Papers

Responses of chlorophyll fluorescence to CO2 elimination as an indicator of Crassulacean acid metabolism photosynthesis Reviewed

Sae Bekki, Kenji Suetsugu, Koichi Kobayashi

Journal of Plant Research 138 323 - 336 2024.12

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Authorship：Last author,　Corresponding author Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

DOI： 10.1007/s10265-024-01608-2
Orchestration of Photosynthesis-Associated Gene Expression and Galactolipid Biosynthesis during Chloroplast Differentiation in Plants.

Sho Fujii, Hajime Wada, Koichi Kobayashi

Plant & cell physiology 65 ( 6 ) 1014 - 1028 2024.06

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Publishing type：Research paper (scientific journal) International / domestic magazine：Domestic journal

The chloroplast thylakoid membrane is composed of membrane lipids and photosynthetic protein complexes, and the orchestration of thylakoid lipid biosynthesis and photosynthesis-associated protein accumulation is considered important for thylakoid development. Galactolipids consist of ∼80% of the thylakoid lipids, and their biosynthesis is fundamental for chloroplast development. We previously reported that the suppression of galactolipid biosynthesis decreased the expression of photosynthesis-associated nuclear-encoded genes (PhAPGs) and photosynthesis-associated plastid-encoded genes (PhAPGs). However, the mechanism for coordinative regulation between galactolipid biosynthesis in plastids and the expression of PhANGs and PhAPGs remains largely unknown. To elucidate this mechanism, we investigated the gene expression patterns in galactolipid-deficient Arabidopsis seedlings during the de-etiolation process. We found that galactolipids are crucial for inducing both the transcript accumulation of PhANGs and PhAPGs and the accumulation of plastid-encoded photosynthesis-associated proteins in developing chloroplasts. Genetic analysis indicates the contribution of the GENOMES UNCOUPLED1 (GUN1)-mediated plastid-to-nucleus signaling pathway to PhANG regulation in response to galactolipid levels. Previous studies suggested that the accumulation of GUN1 reflects the state of protein homeostasis in plastids and alters the PhANG expression level. Thus, we propose a model that galactolipid biosynthesis determines the protein homeostasis in plastids in the initial phase of de-etiolation and optimizes GUN1-dependent signaling to regulate the PhANG expression. This mechanism might contribute to orchestrating the biosynthesis of lipids and proteins for the biogenesis of functional chloroplasts in plants.

DOI： 10.1093/pcp/pcae049

PubMed
Anionic lipids facilitate membrane development and protochlorophyllide biosynthesis in etioplasts.

Akiko Yoshihara, Keiko Kobayashi, Noriko Nagata, Sho Fujii, Hajime Wada, Koichi Kobayashi

Plant physiology 194 ( 3 ) 1692 - 1704 2024.02

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Dark-germinated angiosperm seedlings develop chloroplast precursors called etioplasts in cotyledon cells. Etioplasts develop lattice membrane structures called prolamellar bodies (PLBs), where the chlorophyll intermediate protochlorophyllide (Pchlide) forms a ternary complex with NADPH and light-dependent NADPH:Pchlide oxidoreductase (LPOR). The lipid bilayers of etioplast membranes are mainly composed of galactolipids, which play important roles in membrane-associated processes in etioplasts. Although etioplast membranes also contain 2 anionic lipids, phosphatidylglycerol (PG) and sulfoquinovosyldiacylglycerol (SQDG), their roles are unknown. To determine the roles of PG and SQDG in etioplast development, we characterized etiolated Arabidopsis (Arabidopsis thaliana) mutants deficient in PG and SQDG biosynthesis. A partial deficiency in PG biosynthesis loosened the lattice structure of PLBs and impaired the insertion of Mg2+ into protoporphyrin IX, leading to a substantial decrease in Pchlide content. Although a complete lack of SQDG biosynthesis did not notably affect PLB formation and Pchlide biosynthesis, lack of SQDG in addition to partial PG deficiency strongly impaired these processes. These results suggested that PG is required for PLB formation and Pchlide biosynthesis, whereas SQDG plays an auxiliary role in these processes. Notably, PG deficiency and lack of SQDG oppositely affected the dynamics of LPOR complexes after photoconversion, suggesting different involvements of PG and SQDG in LPOR complex organization. Our data demonstrate pleiotropic roles of anionic lipids in etioplast development.

DOI： 10.1093/plphys/kiad604

PubMed
Biosynthesis of phosphatidylglycerol in photosynthetic organisms.

Koichi Kobayashi, Haruhiko Jimbo, Yuki Nakamura, Hajime Wada

Progress in lipid research 93 101266 - 101266 2024.01

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Phosphatidylglycerol (PG) is a unique phospholipid class with its indispensable role in photosynthesis and growth in land plants, algae, and cyanobacteria. PG is the only major phospholipid in the thylakoid membrane of cyanobacteria and plant chloroplasts and a main lipid component in photosynthetic protein-cofactor complexes such as photosystem I and photosystem II. In plants and algae, PG is also essential as a substrate for the biosynthesis of cardiolipin, which is a unique lipid present only in mitochondrial membranes and crucial for the functions of mitochondria. PG biosynthesis pathways in plants include three membranous organelles, plastids, mitochondria, and the endoplasmic reticulum in a complex manner. While the molecular biology underlying the role of PG in photosynthetic functions is well established, many enzymes responsible for the PG biosynthesis are only recently cloned and functionally characterized in the model plant species including Arabidopsis thaliana and Chlamydomonas reinhardtii and cyanobacteria such as Synechocystis sp. PCC 6803. The characterization of those enzymes helps understand not only the metabolic flow for PG production but also the crosstalk of biosynthesis pathways between PG and other lipids. This review aims to summarize recent advances in the understanding of the PG biosynthesis pathway and functions of involved enzymes.

DOI： 10.1016/j.plipres.2023.101266

PubMed
Biosynthesis of phosphatidylglycerol in photosynthetic organisms. Invited Reviewed

Koichi Kobayashi, Haruhiko Jimbo, Yuki Nakamura, Hajime Wada

Progress in lipid research 93 101266 - 101266 2024.01

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Authorship：Lead author,　Corresponding author Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

DOI： 10.1016/j.plipres.2023
Evolutionary implications from lipids in membrane bilayers and photosynthetic complexes in cyanobacteria and chloroplasts Invited Reviewed

Koichi Kobayashi, Akiko Yoshihara, Hisako Kubota-Kawai

The Journal of Biochemistry 174 ( 5 ) 399 - 408 2023.07

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Authorship：Lead author,　Corresponding author Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

DOI： 10.1093/jb/mvad058
Aerial roots of the leafless epiphytic orchid Taeniophyllum are specialized for performing crassulacean acid metabolism photosynthesis.

Kenji Suetsugu, Ryohei Sugita, Akiko Yoshihara, Hidehito Okada, Kae Akita, Noriko Nagata, Keitaro Tanoi, Koichi Kobayashi

The New phytologist 238 ( 3 ) 932 - 937 2023.02

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International / domestic magazine：International journal

DOI： 10.1111/nph.18812

PubMed
Crucial importance of length of fatty-acyl chains bound to the sn-2 position of phosphatidylglycerol for growth and photosynthesis of Synechocystis sp. PCC 6803 Reviewed

Kaichiro Endo, Masato Abe, Nobumasa Kawanishi, Haruhiko Jimbo, Koichi Kobayashi, Tomoko Suzuki, Noriko Nagata, Hideto Miyoshi, Hajime Wada

Biochimica et Biophysica Acta 1867 ( 7 ) 159158 - 159158 2022.07（ ISSN:1388-1981 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

DOI： 10.1016/j.bbalip.2022.159158
Lipids in photosynthetic protein complexes in the thylakoid membrane of plants, algae, and cyanobacteria. Invited Reviewed

Akiko Yoshihara, Koichi Kobayashi

Journal of experimental botany 73 ( 9 ) 2735 - 2750 2022.05

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Authorship：Last author,　Corresponding author Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

In the thylakoid membrane of cyanobacteria and chloroplasts, many proteins involved in photosynthesis are associated with or integrated into the fluid bilayer matrix formed by four unique glycerolipid classes, monogalactosyldiacylglycerol, digalactosyldiacylglycerol, sulfoquinovosyldiacylglycerol, and phosphatidylglycerol. Biochemical and molecular genetic studies have revealed that these glycerolipids play essential roles not only in the formation of thylakoid lipid bilayers but also in the assembly and functions of photosynthetic complexes. Moreover, considerable advances in structural biology have identified a number of lipid molecules within the photosynthetic complexes such as PSI and PSII. These data have provided important insights into the association of lipids with protein subunits in photosynthetic complexes and the distribution of lipids in the thylakoid membrane. Here, we summarize recent high-resolution observations of lipid molecules in the structures of photosynthetic complexes from plants, algae, and cyanobacteria, and evaluate the distribution of lipids among photosynthetic protein complexes and thylakoid lipid bilayers. By integrating the structural information into the findings from biochemical and molecular genetic studies, we highlight the conserved and differentiated roles of lipids in the assembly and functions of photosynthetic complexes among plants, algae, and cyanobacteria.

DOI： 10.1093/jxb/erac017

PubMed
Impacts of phosphatidylglycerol on plastid gene expression and light induction of nuclear photosynthetic genes. Reviewed International coauthorship

Sho Fujii, Koichi Kobayashi, Ying-Chen Lin, Yu-Chi Liu, Yuki Nakamura, Hajime Wada

Journal of experimental botany 73 ( 9 ) 2952 - 2970 2022.05

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Authorship：Corresponding author Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Phosphatidylglycerol (PG) is the only major phospholipid in the thylakoid membrane of chloroplasts. PG is essential for photosynthesis, and loss of PG in Arabidopsis thaliana results in severe defects of growth and chloroplast development, with decreased chlorophyll accumulation, impaired thylakoid formation, and down-regulation of photosynthesis-associated genes encoded in nuclear and plastid genomes. However, how the absence of PG affects gene expression and plant growth remains unclear. To elucidate this mechanism, we investigated transcriptional profiles of a PG-deficient Arabidopsis mutant pgp1-2 under various light conditions. Microarray analysis demonstrated that reactive oxygen species (ROS)-responsive genes were up-regulated in pgp1-2. However, ROS production was not enhanced in the mutant even under strong light, indicating limited impacts of photooxidative stress on the defects of pgp1-2. Illumination to dark-adapted pgp1-2 triggered down-regulation of photosynthesis-associated nuclear-encoded genes (PhANGs), while plastid-encoded genes were constantly suppressed. Overexpression of GOLDEN2-LIKE1 (GLK1), a transcription factor gene regulating chloroplast development, in pgp1-2 up-regulated PhANGs but not plastid-encoded genes along with chlorophyll accumulation. Our data suggest a broad impact of PG biosynthesis on nuclear-encoded genes partially via GLK1 and a specific involvement of this lipid in plastid gene expression and plant development.

DOI： 10.1093/jxb/erac034

PubMed
Functional divergence of a pair of Arabidopsis phospho-base methyltransferases, PMT1 and PMT3, conferred by distinct N-terminal sequences Reviewed International coauthorship

Yu-Chi Liu, Yue-Rong Tan, Chin-Wen Chang, Van C Nguyen, Kazue Kanehara, Koichi Kobayashi, Yuki Nakamura

The Plant journal 110 ( 4 ) 1198 - 1212 2022.05

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

In seed plants, phospho-base N-methyltransferase (PMT) catalyzes a key step in the biosynthesis pathway of phosphatidylcholine (PC), the most abundant phospholipid class. Arabidopsis thaliana possesses three copies of PMT, with PMT1 and PMT3 play a primary role because the pmt1 pmt3 double mutant shows considerably reduced PC content with a pale seedling phenotype. Although the function of PMT1 and PMT3 may be redundant because neither of the parental single mutants showed a similar mutant phenotype, major developmental defects and possible functional divergence of these PMTs underlying the pale pmt1 pmt3 seedling phenotype are unknown. Here, we show the major developmental defect of the pale seedlings in xylem of the hypocotyl with partial impairments in chloroplast development and photosynthetic activity in leaves. Although PMT1 and PMT3 are localized at the endoplasmic reticulum, their tissue-specific expression pattern was distinct in hypocotyls and roots. Intriguingly, the function of PMT3 but not PMT1 requires its characteristic N-terminal sequence in addition to the promoter because truncation of the N-terminal sequence of PMT3 or substitution with PMT1 driven by the PMT3 promoter failed to rescue the pale pmt1 pmt3 seedling phenotype. Thus, PMT3 function requires the N-terminal sequence in addition to its promoter, whereas the PMT1 function is defined by the promoter.

DOI： 10.1111/tpj.15741

PubMed
Photosynthesis and cell growth trigger degradation of phycobilisomes during nitrogen limitation Reviewed

Akiko Yoshihara, Koichi Kobayashi

Plant and Cell Physiology 63 ( 2 ) 189 - 199 2021.10

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Authorship：Last author,　Corresponding author Publishing type：Research paper (scientific journal) Kind of work：Joint Work International / domestic magazine：International journal

Under nitrogen (N)-limited conditions, the non-N2-fixing cyanobacterium Synechocystis sp. PCC 6803 (Synechocystis 6803) actively grows during early stages of starvation by performing photosynthesis but gradually stops the growth and eventually enters dormancy to withstand long-term N limitation. During N limitation, Synechocystis 6803 cells degrade the large light-harvesting antenna complex phycobilisomes (PBSs) presumably to avoid excess light absorption and to reallocate available N to essential functions for growth and survival. These two requirements may be driving forces for PBS degradation during N limitation, but how photosynthesis and cell growth affect PBS degradation remains unclear. To address this question, we examined involvements of photosynthesis and cell growth in PBS degradation during N limitation. Treatment of photosynthesis inhibitors and shading suppressed PBS degradation and caused non-bleaching of cells during N limitation. Limitations of photosynthesis after initial gene responses to N limitation suppressed PBS degradation, implying that photosynthesis affects PBS degradation in a post-translational manner. In addition, limitations of cell growth by inhibition of peptidoglycan and fatty acid biosynthesis, low growth temperature and phosphorous starvation suppressed PBS degradation during N limitation. Because decreased photosynthetic activity led to decreased cell growth, and vice versa, photosynthesis and cell growth would inseparably intertwine each other and affect PBS degradation during N limitation in a complex manner. Our data shed light on the coordination mechanisms among photosynthesis, cell growth and PBS degradation during N limitation.

DOI： 10.1093/pcp/pcab159

PubMed
Plastid anionic lipids are essential for the development of both photosynthetic and non-photosynthetic organs in Arabidopsis thaliana Reviewed

Akiko Yoshihara, Noriko Nagata, Hajime Wada, Koichi Kobayashi

International Journal of Molecular Sciences 22 ( 9 ) 4860 - 4860 2021.05（ eISSN:1422-0067 ）

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Publishing type：Research paper (scientific journal) Kind of work：Joint Work International / domestic magazine：International journal

The lipid bilayer matrix of the thylakoid membrane of cyanobacteria and chloroplasts of plants and algae is mainly composed of uncharged galactolipids, but also contains anionic lipids sulfoquinovosyldiacylglycerol (SQDG) and phosphatidylglycerol (PG) as major constituents. The necessity of PG for photosynthesis is evident in all photosynthetic organisms examined to date, whereas the requirement of SQDG varies with species. In plants, although PG and SQDG are also found in non-photosynthetic plastids, their importance for the growth and functions of non-photosynthetic organs remains unclear. In addition, plants synthesize another anionic lipid glucuronosyldiacylglycerol (GlcADG) during phosphorus starvation, but its role in plant cells is not elucidated yet. To understand the functional relationships among PG, SQDG, and GlcADG, we characterized several Arabidopsis thaliana mutants defective in biosynthesis of these lipids. The mutants completely lacking both PG and SQDG biosynthesis in plastids showed developmental defects of roots, hypocotyls, and embryos in addition to leaves, which suggests that these lipids are pleiotropically required for the development of both photosynthetic and non-photosynthetic organs. Furthermore, our analysis revealed that SQDG, but not GlcADG, is essential for complementing the role of PG, particularly in photosynthesis under PG-deficient conditions such as phosphorus starvation.

DOI： 10.3390/ijms22094860
A START domain-containing protein is involved in the incorporation of ER-derived fatty acids into chloroplast glycolipids in Marchantia polymorpha Reviewed

Takashi Hirashima, Haruhiko Jimbo, Koichi Kobayashi, Hajime Wada

Biochemical and Biophysical Research Communications 534 ( 1 ) 436 - 441 2021.01

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Publishing type：Research paper (scientific journal) Kind of work：Joint Work International / domestic magazine：International journal

The appropriate regulation of thylakoid lipid synthesis is essential for the function of chloroplasts. In plant cells, membrane lipids synthesized in the ER are utilized as a precursor for the synthesis of chloroplast glycolipids. This pathway is thought to be mediated by the transport of glycerolipids synthesized in the ER into chloroplasts. However, we have little knowledge about the proteins involved in the lipid transfer between these organelles in plant cells. Here we show a protein, STAR2, containing the START (Steroidogenic acute regulatory protein-related lipid transfer) domain known to function as a lipid transporter, is involved in the incorporation of ER-derived fatty acids into chloroplast glycolipids in Marchantia polymorpha. We found that STAR2 localizes on the chloroplast envelope membrane as a punctuate structure and is required for the increase of C20 fatty acids, which are synthesized in the ER, in chloroplast glycolipids in response to phosphate deprivation. Our results indicate that STAR2 of M. polymorpha is likely to be involved in the lipid transfer from ER to chloroplast, presumably as a lipid transporter.

DOI： 10.1016/j.bbrc.2020.11.063

PubMed
The leafless orchid cymbidium macrorhizon performs photosynthesis in pericarp during fruiting season Reviewed OA

Koichi Kobayashi, Kenji Suetsugu, Hajime Wada

Plant and Cell Physiology 62 ( 3 ) 472 - 481 2021.01

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Authorship：Lead author,　Corresponding author Publishing type：Research paper (scientific journal) Kind of work：Joint Work International / domestic magazine：International journal

Photosynthesis with highly photoreactive chlorophyll (Chl) provides energy for plant growth but with simultaneous risk of photooxidative damage and photoprotection costs. Although the leafless orchid Cymbidium macrorhizon mostly depends on mycorrhizal fungi for carbon, it accumulates Chl particularly during fruiting and may not be fully mycoheterotrophic. In fact, stable isotopic analysis suggested that the fruiting C. macrorhizon specimens obtain a significant proportion of its carbon demands through photosynthesis. However, actual photosynthetic characteristics of this leafless orchid are unknown. To reveal the functionality of photosynthetic electron transport in C. macrorhizon, we compared its photosynthetic properties with those of its relative mixotrophic orchid C. goeringii and the model plant Arabidopsis thaliana. Compared with C. goeringii and A. thaliana, maximum photochemical efficiency of PSII was substantially low in C. macrorhizon. Chl fluorescence induction kinetics revealed that the electron transport capacity of PSII was limited in C. macrorhizon. Chl fluorescence analysis at 77 K suggested partial energetic disconnection of the light-harvesting antenna from the PSII reaction center in C. macrorhizon. Despite its low PSII photochemical efficiency, C. macrorhizon showed photosynthetic electron-transport activity both in the field and under laboratory conditions. C. macrorhizon developed strong non-photochemical quenching in response to increased light intensity as did C. goeringii, suggesting the functionality of photoprotective systems in this orchid. Moreover, C. macrorhizon fruit developed stomata on the pericarp and showed net O2-evolving activity. Our data demonstrate that C. macrorhizon can perform photosynthetic electron transport in the pericarp, although its contribution to net carbon acquisition may be limited.

DOI： 10.1093/pcp/pcab006

PubMed

Repository URL： http://hdl.handle.net/10466/00017656
The Endoplasmic Reticulum Pathway for Membrane Lipid Synthesis Has a Significant Contribution toward Shoot Removal-Induced Root Chloroplast Development in Arabidopsis Reviewed

Tomoki Obata, Koichi Kobayashi, Ryosuke Tadakuma, Taiki Akasaka, Koh Iba, Juntaro Negi

Plant and Cell Physiology 62 ( 3 ) 494 - 501 2021.01

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Publishing type：Research paper (scientific journal) Kind of work：Joint Work International / domestic magazine：International journal

Chloroplast lipids are synthesized via two distinct pathways: the plastidic pathway and endoplasmic reticulum (ER) pathway. We previously reported that the contribution of the two pathways toward chloroplast development is different between mesophyll cells and guard cells in Arabidopsis leaf tissues, and that the ER pathway plays a major role in guard cell chloroplast development. However, little is known about the contribution of the two pathways toward chloroplast development in other tissue cells, and in this study, we focused on the root cells. Chloroplast development is normally repressed in roots but can be induced when the roots are detached from the shoots (root greening). We found that similar to guard cells, root cells exhibit a higher proportion of glycolipid from the ER pathway. Root greening was repressed in the gles1 mutant, which has a defect in ER-to-plastid lipid transportation via the ER pathway, while normal root greening was observed in the ats1 mutant, whose plastidic pathway is blocked. Lipid analysis revealed that the gles1 mutation caused drastic decrease in the ER-derived glycolipids in roots. Furthermore, the gles1 detached roots showed smaller chloroplasts containing little starch than WT. These results suggest that the ER pathway has a significant contribution toward chloroplast development in the root cells.

DOI： 10.1093/pcp/pcab009

PubMed
An Artificial Conversion of Roots into Organs with Shoot Stem Characteristics by Inducing Two Transcription Factors.

Shigeru Hanano, Hajime Tomatsu, Ai Ohnishi, Koichi Kobayashi, Yuki Kondo, Shigeyuki Betsuyaku, Eiji Takita, Yoshiyuki Ogata, Keishi Ozawa, Kunihiro Suda, Tsutomu Hosouchi, Takahiro Nagase, Hideyuki Suzuki, Nozomu Sakurai, Hiroshi Masumoto, Hiroo Fukuda, Daisuke Shibata

iScience 23 ( 7 ) 101332 - 101332 2020.07

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Somatic plant cells can regenerate shoots and/or roots or adventitious embryonic calluses, which may induce organ formation under certain conditions. Such regenerations occur via dedifferentiation of somatic cells, induction of organs, and their subsequent outgrowth. Despite recent advances in understanding of plant regeneration, many details of shoot induction remain unclear. Here, we artificially induced shoot stem-like green organs (SSOs) in Arabidopsis thaliana roots via simultaneous induction of two transcription factors (TFs), ARABIDOPSIS THALIANA HOMEOBOX PROTEIN 25 (ATHB25, At5g65410) and the B3 family transcription factor REPRODUCTIVE MERISTEM 7 (REM7, At3g18960). The SSOs exhibited negative gravitropism and differentiated vascular bundle phenotypes. The ATHB25/REM7 induced the expression of genes controlling shoot stem characteristics by ectopic expression in roots. Intriguingly, the restoration of root growth was seen in the consecutive and adjacent parts of the SSOs under gene induction conditions. Our findings thus provide insights into the development and regeneration of plant shoot stems.

DOI： 10.1016/j.isci.2020.101332

PubMed
Relationship Between Glycerolipids and Photosynthetic Components During Recovery of Thylakoid Membranes From Nitrogen Starvation-Induced Attenuation in Synechocystis sp. PCC 6803.

Koichi Kobayashi, Yuka Osawa, Akiko Yoshihara, Mie Shimojima, Koichiro Awai

Frontiers in plant science 11 432 - 432 2020

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Thylakoid membranes, the site of photochemical and electron transport reactions of oxygenic photosynthesis, are composed of a myriad of proteins, cofactors including pigments, and glycerolipids. In the non-diazotrophic cyanobacterium Synechocystis sp. PCC 6803, the size and function of thylakoid membranes are reduced under nitrogen (N) starvation but are quickly recovered after N addition to the starved cells. To understand how the functionality of thylakoid membranes is adjusted in response to N status in Synechocystis sp. PCC 6803, we examined changes in thylakoid components and the photosynthetic activity during the N starvation and recovery processes. In N-starved cells, phycobilisome content, photosystem II protein levels and the photosynthetic activity substantially decreased as compared with those in N-sufficient cells. Although the content of chlorophyll (Chl) a, total protein and total glycerolipid also decreased under the N-starved condition based on OD730 reflecting cell density, when based on culture volume, the Chl a and total protein content remained almost constant and total glycerolipid content even increased during N starvation, suggesting that cellular levels of these components decrease under the N-starved condition mainly through dilution due to cell growth. With N addition, the photosynthetic activity quickly recovered, followed by full restoration of photosynthetic pigment and protein levels. The content of phosphatidylglycerol (PG), an essential lipid constituent of both photosystems, increased faster than that of Chl a, whereas the content of glycolipids, the main constituents of the thylakoid lipid bilayer, gradually recovered after N addition. The data indicate differential regulation of PG and glycolipids during the construction of the photosynthetic machinery and regeneration of thylakoid membranes. Of note, addition of PG to the growth medium slightly accelerated the Chl a accumulation in wild-type cells during the recovery process. Because PG is required for the biosynthesis of Chl a and the formation of functional photosystem complexes, rapid PG biosynthesis in response to N acquisition may be required for the rapid formation of the photosynthetic machinery during thylakoid regeneration.

DOI： 10.3389/fpls.2020.00432

PubMed
Role of Galactolipids in Plastid Differentiation Before and After Light Exposure. Reviewed

Fujii S, Wada H, Kobayashi K

Plants (Basel, Switzerland) 8 ( 10 ) 2019.09

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Galactolipids, monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG), are the predominant lipid classes in the thylakoid membrane of chloroplasts. These lipids are also major constituents of internal membrane structures called prolamellar bodies (PLBs) and prothylakoids (PTs) in etioplasts, which develop in the cotyledon cells of dark-grown angiosperms. Analysis of Arabidopsis mutants defective in the major galactolipid biosynthesis pathway revealed that MGDG and DGDG are similarly and, in part, differently required for membrane-associated processes such as the organization of PLBs and PTs and the formation of pigment-protein complexes in etioplasts. After light exposure, PLBs and PTs in etioplasts are transformed into the thylakoid membrane, resulting in chloroplast biogenesis. During the etioplast-to-chloroplast differentiation, galactolipids facilitate thylakoid membrane biogenesis from PLBs and PTs and play crucial roles in chlorophyll biosynthesis and accumulation of light-harvesting proteins. These recent findings shed light on the roles of galactolipids as key facilitators of several membrane-associated processes during the development of the internal membrane systems in plant plastids.

DOI： 10.3390/plants8100357

PubMed
Galactolipids are essential for internal membrane transformation during etioplast-to-chloroplast differentiation. Reviewed

Fujii S, Nagata N, Masuda T, Wada H, Kobayashi K

Plant & cell physiology 60 ( 6 ) 1224 - 1238 2019.03（ ISSN:0032-0781 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：Domestic journal

Etioplasts developed in angiosperm cotyledon cells in darkness rapidly differentiate into chloroplasts with illumination. This process involves dynamic transformation of internal membrane structures from the prolamellar bodies (PLBs) and prothylakoids (PTs) in etioplasts to thylakoid membranes in chloroplasts. Although two galactolipids, monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG), are predominant lipid constituents of membranes in both etioplasts and chloroplasts, their roles in the structural and functional transformation of internal membranes during etioplast-to-chloroplast differentiation are unknown. We previously reported that a 36% loss of MGDG by an artificial microRNA targeting major MGDG synthase (amiR-MGD1) only slightly affected PLB structures but strongly impaired PT formation and protochlorophyllide biosynthesis. Meanwhile, strong DGDG deficiency in a DGDG synthase mutant (dgd1) disordered the PLB lattice structure in addition to impaired PT development and protochlorophyllide biosynthesis. In this study, thylakoid biogenesis after PLB disassembly with illumination was strongly perturbed by amiR-MGD1. The amiR-MGD1 expression impaired the accumulation of Chl and the major light-harvesting complex II protein (LHCB1), which may inhibit rapid transformation from disassembled PLBs to the thylakoid membrane. As did amiR-MGD1 expression, dgd1 mutation impaired the accumulation of Chl and LHCB1 during etioplast-to-chloroplast differentiation. Furthermore, unlike in amiR-MGD1 seedlings, in dgd1 seedlings, disassembly of PLBs after illumination was retarded. Because DGDG but not MGDG prefers to form the bilayer lipid phase in membranes, the MGDG-to-DGDG ratio may strongly affect the transformation of PLBs to the thylakoid membrane during etioplast-to-chloroplast differentiation.

DOI： 10.1093/pcp/pcz041

PubMed
Site-directed mutagenesis of two amino acid residues in cytochrome b 559 α subunit that interact with a phosphatidylglycerol molecule (PG772) induces quinone-dependent inhibition of photosystem II activity Reviewed

Kaichiro Endo, Koichi Kobayashi, Hsing-Ting Wang, Hsiu-An Chu, Jian-Ren Shen, Hajime Wada

Photosynthesis research 雑誌 139 267 - 279 2019.01

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Kind of work：Joint Work
Blue Light Regulates Phosphate Deficiency-Dependent Primary Root Growth Inhibition in Arabidopsis. Reviewed

Chuan-Ming Yeh, Koichi Kobayashi, Sho Fujii, Hidehiro Fukaki, Nobutaka Mitsuda, Masaru Ohme-Takagi

Frontiers in plant science 10 1803 - 1803 2019

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Plants have evolved mechanisms to improve utilization efficiency or acquisition of inorganic phosphate (Pi) in response to Pi deficiency, such as altering root architecture, secreting acid phosphatases, and activating the expression of genes related to Pi uptake and recycling. Although many genes responsive to Pi starvation have been identified, transcription factors that affect tolerance to Pi deficiency have not been well characterized. We show here that the ectopic expression of B-BOX32 (BBX32) and the mutation of ELONGATED HYPOCOTYL 5 (HY5), whose transcriptional activity is negatively regulated by BBX32, resulted in the tolerance to Pi deficiency in Arabidopsis. The primary root lengths of 35S:BBX32 and hy5 plants were only slightly inhibited under Pi deficient condition and the fresh weights were significantly higher than those of wild type. The Pi deficiency-tolerant root phenotype of hy5 was similarly observed when grown on the medium without Pi. In addition, a double mutant, hy5 slr1, without lateral roots, also showed a long primary root phenotype under phosphate deficiency, indicating that the root phenotype of hy5 does not result from an increase of external Pi uptake. Moreover, we found that blue light may regulate Pi deficiency-dependent primary root growth inhibition through activating peroxidase gene expression, suggesting the Pi-deficiency tolerant root phenotype of hy5 may be due to blockage of blue light responses. Altogether, this study points out light quality may play an important role in the regulation of Pi deficiency responses. It may contribute to regulate plant growth under Pi deficiency through proper illumination.

DOI： 10.3389/fpls.2019.01803

PubMed
A Novel Conversion of Roots into Organs with Shoot Stem Characteristics by Inducing Two Transcription Factors

Shigeru Hanano, Hajime Tomatsu, Ai Ohnishi, Koichi Kobayashi, Yuki Kondo, Shigeyuki Betsuyaku, Eiji Takita, Yoshiyuki Ogata, Keishi Ozawa, Kunihiro Suda, Tsutomu Hosouchi, Takahiro Nagase, Hideyuki Suzuki, Nozomu Sakurai, Hiroo Fukuda, Daisuke Shibata

SSRN Electronic Journal 2019（ eISSN:1556-5068 ）

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Publishing type：Research paper (scientific journal)

DOI： 10.2139/ssrn.3387683
Role of galactolipids in etioplast development and etioplast-to-chloroplast differentiation Reviewed

28 139 - 147 2018.12

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Kind of work：Joint Work
High myristic acid content in the cyanobacterium Cyanothece sp. PCC 8801 results from substrate specificity of lysophosphatidic acid Reviewed

Masakazu Saito, Kaichiro Endo, Koichi Kobayashi, Mai Watanabe, Masahiko Ikeuchi, Akio Murakami, Norio Murata, Hajime Wada

Biochimica et Biophysica Acta 雑誌 1863 939 - 947 2018.09

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Thylakoid membrane lipid sulfoquinovosyl-diacylglycerol (SQDG) is required for full functioning of photosystem II in Thermosynechococcus elongatus Reviewed

Yoshiki Nakajima, Yasufumi Umena, Ryo Nagao, Kaichiro Endo, Koichi Kobayashi, Fusamichi Akita, Michihiro Suga, Hajime Wada, Takumi Noguchi, Jian-Ren Shen

Journal of Biological Chemistry 雑誌 293 14786 - 14797 2018.09

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Digalactosyldiacylglycerol is essential for organization of the membrane structure in etioplasts Reviewed

Sho Fujii, Koichi Kobayashi, Noriko Nagata, Tatsuru Masuda, Hajime Wada

Plant physiology 雑誌 177 1487 - 1497 2018.08

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Phosphatidylglycerophosphate phosphatase is required for root growth in Arabidopsis Reviewed

Ying-Chen Lin, Koichi Kobayashi, Hajime Wada, Yuki Nakamura

Biochimica et Biophysica Acta 雑誌 1863 563 - 575 2018.06

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Improved photosynthesis in Arabidopsis roots by activation of GATA transcription factors Reviewed

Ai Ohnishi, Hajime Wada, Koichi Kobayashi

Photosynthetica 雑誌 56 433 - 444 2018.03

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Specific Distribution of Phosphatidylglycerol to Photosystem Complexes in the Thylakoid Membrane Reviewed

Koichi Kobayashi, Kaichiro Endo, Hajime Wada

Frontiers in plant science 雑誌 8 2017.11

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Monogalactosyldiacylglycerol facilitates synthesis of photoactive protochlorophyllide in etioplasts Reviewed

Sho Fujii, Koichi Kobayashi, Noriko Nagata, Tatsuru Masuda, Hajime Wada

Plant physiology 雑誌 174 2183 - 2198 2017.08

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Simultaneous but spatially different regulation of non-photosynthetic callus formation and photosynthetic root development after shoot removal Reviewed

Koichi Kobayashi, Akira Iwase

Plant signaling & behavior 雑誌 12 2340 - 2355 2017.06

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Shoot removal induces chloroplast development in roots via cytokinin signaling Reviewed

Koichi Kobayashi, Ai Ohnishi, Daichi Sasaki, Sho Fujii, Akira Iwase, Keiko Sugimoto, Tatsuru Masuda, Hajime Wada

Plant physiology 雑誌 173 2340 - 2355 2017.04

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Transcriptional regulation of tetrapyrrole biosynthesis in Arabidopsis thaliana Reviewed

Koichi Kobayashi, Tatsuru Masuda

Frontiers in plant science 雑誌 7 2016.12

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Kind of work：Joint Work
Arabidopsis phosphatidylglycerophosphate phosphatase 1 involved in phosphatidylglycerol biosynthesis and photosynthetic function Reviewed

Ying‐Chen Lin, Koichi Kobayashi, Chun‐Hsien Hung, Hajime Wada, Yuki Nakamura

The Plant Journal 雑誌 88 1022 - 1037 2016.12

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Kind of work：Joint Work
CyanoBase: a large-scale update on its 20th anniversary Reviewed

Takatomo Fujisawa, Rei Narikawa, Shin-ichi Maeda, Satoru Watanabe, Yu Kanesaki, Koichi Kobayashi, Jiro Nomata, Mitsumasa Hanaoka, Mai Watanabe, Shigeki Ehira, Eiji Suzuki, Koichiro Awai, Yasukazu Nakamura

Nucleic acids research 雑誌 45 D551 - D554 2016.11

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Kind of work：Joint Work
Sulfoquinovosyldiacylglycerol has an essential role in Thermosynechococcus elongatus BP-1 under phosphate-deficient conditions Reviewed

Kaichiro Endo, Koichi Kobayashi, Hajime Wada

Plant and Cell Physiology 雑誌 57 2461 - 2471 2016.09

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Kind of work：Joint Work
Allocation of heme is differentially regulated by ferrochelatase isoforms in Arabidopsis cells Reviewed

Nino A Espinas, Koichi Kobayashi, Yasushi Sato, Nobuyoshi Mochizuki, Kaori Takahashi, Ryouichi Tanaka, Tatsuru Masuda

Frontiers in plant science 雑誌 7 2016.08

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Kind of work：Joint Work
Role of membrane glycerolipids in photosynthesis, thylakoid biogenesis and chloroplast development Reviewed

Koichi Kobayashi

Journal of plant research 雑誌 129 565 - 580 2016.07

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Kind of work：Single Work
Multiple impacts of loss of plastidic phosphatidylglycerol biosynthesis on photosynthesis during seedling growth of Arabidopsis Reviewed

Koichi Kobayashi, Kaichiro Endo, Hajime Wada

Frontiers in plant science 雑誌 7 2016.03

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Kind of work：Joint Work
Functional specificity of cardiolipin synthase revealed by the identification of a cardiolipin synthase CrCLS1 in Chlamydomonas reinhardtii Reviewed

Chun-Hsien Hung, Koichi Kobayashi, Hajime Wada, Yuki Nakamura

Frontiers in microbiology 雑誌 6 2016.01

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Kind of work：Joint Work
Site-directed mutagenesis of amino acid residues of D1 protein interacting with phosphatidylglycerol affects the function of plastoquinone QB in photosystem II Reviewed

Kaichiro Endo, Naoki Mizusawa, Jian-Ren Shen, Masato Yamada, Tatsuya Tomo, Hirohisa Komatsu, Masami Kobayashi, Koichi Kobayashi, Hajime Wada

Photosynthesis research 雑誌 126 385 - 397 2015.12

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Biosynthesis of Thylakoid Membrane Lipids in Plants and Their Roles in Chloroplast Biogenesis Reviewed

25 126 - 137 2015.08

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Kind of work：Single Work
Lipids in Photosynthetic Protein Complexes Reviewed

25 116 - 125 2015.08

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Kind of work：Joint Work
Characterization of Chlamydomonas reinhardtii phosphatidylglycerophosphate synthase in Synechocystis sp. PCC 6803 Reviewed

Chun-Hsien Hung, Kaichiro Endo, Koichi Kobayashi, Yuki Nakamura, Hajime Wada

Frontiers in microbiology 雑誌 6 2015.08

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Kind of work：Joint Work
Isolation and characterization of a phosphatidylglycerophosphate phosphatase1, PGPP1, in Chlamydomonas reinhardtii Reviewed

Chun-Hsien Hung, Koichi Kobayashi, Hajime Wada, Yuki Nakamura

Plant Physiology and Biochemistry 雑誌 92 56 - 61 2015.07

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Kind of work：Joint Work
Specific role of phosphatidylglycerol and functional overlaps with other thylakoid lipids in Arabidopsis chloroplast biogenesis Reviewed

Koichi Kobayashi, Sho Fujii, Mayuko Sato, Kiminori Toyooka, Hajime Wada

Plant cell reports 雑誌 2015.04

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Kind of work：Joint Work
Expression Analysis of Transcription Factors Involved in Chloroplast Differentiation Reviewed

Dwi Andi Listiawan, Ryo Tanoue, Koichi Kobayashi, Tatsuru Masuda

Procedia Chemistry 雑誌 14 146 - 151 2015.01

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Kind of work：Joint Work
Inducible knockdown of MONOGALACTOSYLDIACYLGLYCEROL SYNTHASE1 reveals roles of galactolipids in organelle differentiation in Arabidopsis cotyledons Reviewed

Sho Fujii, Koichi Kobayashi, Yuki Nakamura, Hajime Wada

Plant physiology 雑誌 166 1436 - 1449 2014.11

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Kind of work：Joint Work
Molecular phylogeny and intricate evolutionary history of the three isofunctional enzymes involved in the oxidation of protoporphyrinogen IX Reviewed

Koichi Kobayashi, Tatsuru Masuda, Naoyuki Tajima, Hajime Wada, Naoki Sato

Genome biology and evolution 雑誌 6 2141 - 2155 2014.08

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Kind of work：Joint Work
Monogalactosyldiacylglycerol synthesis in the outer envelope membrane of chloroplasts is required for enhanced growth under sucrose supplementation Reviewed

Masato Murakawa, Mie Shimojima, Yuichi Shimomura, Koichi Kobayashi, Koichiro Awai, Hiroyuki Ohta

Frontiers in Plant Science 雑誌 5 2014.06

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Kind of work：Joint Work
Transcriptional regulation of thylakoid galactolipid biosynthesis coordinated with chlorophyll biosynthesis during the development of chloroplasts in Arabidopsis Reviewed

Koichi Kobayashi, Sho Fujii, Daichi Sasaki, Shinsuke Baba, Hiroyuki Ohta, Tatsuru Masuda, Hajime Wada

Frontiers in Plant Science 雑誌 5 2014.06

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Kind of work：Joint Work
Klebsormidium flaccidum genome reveals primary factors for plant terrestrial adaptation Reviewed

Koichi Hori, Fumito Maruyama, Takatomo Fujisawa, Tomoaki Togashi, Nozomi Yamamoto, Mitsunori Seo, Syusei Sato, Takuji Yamada, Hiroshi Mori, Naoyuki Tajima, Takashi Moriyama, Masahiko Ikeuchi, Mai Watanabe, Hajime Wada, Koichi Kobayashi, Masakazu Saito, Tatsuru Masuda, Yuko Sasaki-Sekimoto, Kiyoshi Mashiguchi, Koichiro Awai, Mie Shimojima, Shinji Masuda, Masako Iwai, Takashi Nobusawa, Takafumi Narise, Satoshi Kondo, Hikaru Saito, Ryoichi Sato, Masato Murakawa, Yuta Ihara, Yui Oshima-Yamada, Kinuka Ohtaka, Masanori Satoh, Kohei Sonobe, Midori Ishii, Ryosuke Ohtani, Miyu Kanamori-Sato, Rina Honoki, Daichi Miyazaki, Hitoshi Mochizuki, Jumpei Umetsu, Kouichi Higashi, Daisuke Shibata, Yuji Kamiya, Naoki Sato, Yasukazu Nakamura, Satoshi Tabata, Shigeru Ida, Ken Kurokawa, Hiroyuki Ohta

Nature communications 雑誌 5 2014.05

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Kind of work：Joint Work
Photosynthesis of root chloroplasts developed in Arabidopsis lines overexpressing GOLDEN2-LIKE transcription factors

Koichi Kobayashi, Daichi Sasaki, Ko Noguchi, Daiki Fujinuma, Hirohisa Komatsu, Masami Kobayashi, Mayuko Sato, Kiminori Toyooka, Keiko Sugimoto, Krishna K Niyogi, Hajime Wada, Tatsuru Masuda

Plant and cell physiology 54 1365 - 1377 2013.06
Role of galactolipid biosynthesis in coordinated development of photosynthetic complexes and thylakoid membranes during chloroplast biogenesis in Arabidopsis Reviewed

Koichi Kobayashi, Takafumi Narise, Kintake Sonoike, Haruki Hashimoto, Naoki Sato, Maki Kondo, Mikio Nishimura, Mayuko Sato, Kiminori Toyooka, Keiko Sugimoto, Hajime Wada, Tatsuru Masuda, Hiroyuki Ohta

The Plant Journal 雑誌 73 250 - 261 2013.01

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Kind of work：Joint Work
Regulation of Chlorophyll Biosynthesis in Higher Plants Reviewed

22 125 - 138 2012.08

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Kind of work：Single Work
Role of the G-box element in regulation of chlorophyll biosynthesis in arabidopsis roots Reviewed

Koichi Kobayashi, Takeshi Obayashi, Tatsuru Masuda

Plant Signaling & Behavior 雑誌 7 11 - 10 2012.08

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Kind of work：Joint Work
Evaluation of Unbound Free Heme in Plant Cells by Differential Acetone Extraction Reviewed

Nino A Espinas, Koichi Kobayashi, Shigekazu Takahashi, Nobuyoshi Mochizuki, Tatsuru Masuda

Plant and Cell Physiology 雑誌 53 1344 - 1354 2012.07

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Kind of work：Joint Work
Regulation of root greening by light and auxin/cytokinin signaling in Arabidopsis Reviewed

Koichi Kobayashi, Shinsuke Baba, Takeshi Obayashi, Mayuko Sato, Kiminori Toyooka, Mika Keränen, Eva-Mari Aro, Hidehiro Fukaki, Hiroyuki Ohta, Keiko Sugimoto, Tatsuru Masuda

The Plant Cell 雑誌 24 1081 - 1095 2012.03

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Kind of work：Joint Work
Deficiency in riboflavin biosynthesis affects tetrapyrrole biosynthesis in etiolated Arabidopsis tissue Reviewed

Boris Hedtke, Ali Alawady, Alfonso Albacete, Koichi Kobayashi, Michael Melzer, Thomas Roitsch, Tatsuru Masuda, Bernhard Grimm

Plant molecular biology 雑誌 78 77 - 93 2012.01

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Kind of work：Joint Work
Tetrapyrrole metabolism in Arabidopsis thaliana Reviewed

Ryouichi Tanaka, Koichi Kobayashi, Tatsuru Masuda

The Arabidopsis book 著書 9 2011.07

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Kind of work：Joint Work
Involvement of auxin signaling mediated by IAA14 and ARF7/19 in membrane lipid remodeling during phosphate starvation Reviewed

Takafumi Narise, Koichi Kobayashi, Shinsuke Baba, Mie Shimojima, Shinji Masuda, Hidehiro Fukaki, Hiroyuki Ohta

Plant molecular biology 雑誌 72 533 - 544 2010.03

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Kind of work：Joint Work
Galactolipid Synthesis and Its Roles in Higher Plants Reviewed

19 52 - 58 2009.08

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Kind of work：Single Work
Activation of galactolipid biosynthesis in development of pistils and pollen tubes Reviewed

Yuki Nakamura, Koichi Kobayashi, Hiroyuki Ohta

Plant Physiology and Biochemistry 雑誌 47 535 - 539 2009.06

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Kind of work：Joint Work
Type A and type B monogalactosyldiacylglycerol synthases are spatially and functionally separated in the plastids of higher plants

Koichi Kobayashi, Yuki Nakamura, Hiroyuki Ohta

Plant Physiology and Biochemistry 雑誌 47 518 - 525 2009.06

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Kind of work：Joint Work
Galactolipid synthesis and its role in membrane biogenesis in higher plants Reviewed

21 29 - 39 2009.04

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Kind of work：Joint Work
Type‐B monogalactosyldiacylglycerol synthases are involved in phosphate starvation‐induced lipid remodeling, and are crucial for low‐phosphate adaptation Reviewed

Koichi Kobayashi, Koichiro Awai, Masanobu Nakamura, Akira Nagatani, Tatsuru Masuda, Hiroyuki Ohta

The Plant Journal 雑誌 57 322 - 331 2009.01

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Kind of work：Joint Work
Functional analysis of Arabidopsis thaliana isoforms of the Mg-chelatase CHLI subunit Reviewed

Koichi Kobayashi, Nobuyoshi Mochizuki, Naho Yoshimura, Ken Motohashi, Toru Hisabori, Tatsuru Masuda

Photochemical & Photobiological Sciences 雑誌 7 1188 - 1195 2008.06

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Kind of work：Joint Work
葉緑体チラコイド膜を構成するグリセロ糖脂質の生合成と機能

下嶋美恵，小林康一，太田啓之

化学と生物雑誌 46 330 - 338 2008.05

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Kind of work：Joint Work
Galactolipid synthesis in chloroplast inner envelope is essential for proper thylakoid biogenesis, photosynthesis, and embryogenesis Reviewed

Koichi Kobayashi, Maki Kondo, Hiroaki Fukuda, Mikio Nishimura, Hiroyuki Ohta

Proceedings of the National Academy of Sciences of USA 雑誌 2007.10

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Kind of work：Joint Work
Membrane lipid alteration during phosphate starvation is regulated by phosphate signaling and auxin/cytokinin cross‐talk Reviewed

Koichi Kobayashi, Tatsuru Masuda, Ken‐ichiro Takamiya, Hiroyuki Ohta

The Plant Journal 雑誌 47 238 - 248 2006.07

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Kind of work：Joint Work
12-oxo-phytodienoic acid triggers expression of a distinct set of genes and plays a role in wound-induced gene expression in Arabidopsis Reviewed

Nozomi Taki, Yuko Sasaki-Sekimoto, Takeshi Obayashi, Akihiro Kikuta, Koichi Kobayashi, Takayuki Ainai, Kaori Yagi, Nozomu Sakurai, Hideyuki Suzuki, Tatsuru Masuda, Ken-ichiro Takamiya, Daisuke Shibata, Yuichi Kobayashi, Hiroyuki Ohta

Plant physiology 雑誌 139 1268 - 1283 2005.11

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Kind of work：Joint Work
リン酸欠乏時における膜脂質合成系の代謝変動と植物ホルモンによる制御

小林康一, 太田啓之

日本農芸化学会誌雑誌 78 977 - 980 2004.10

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Kind of work：Joint Work
Arabidopsis type B monogalactosyldiacylglycerol synthase genes are expressed during pollen tube growth and induced by phosphate starvation Reviewed

Koichi Kobayashi, Koichiro Awai, Ken-ichiro Takamiya, Hiroyuki Ohta

Plant physiology 雑誌 134 640 - 648 2004.02

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Kind of work：Joint Work

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Books and Other Publications

光合成

高橋　裕一郎，園池　公毅，古本　強　他（ Role： Joint author）

朝倉書店 2021.12 （ ISBN:ISBN978-4-254-17176- ）

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Responsible for pages：16-19
Transcriptional control for the chlorophyll metabolism

Koichi Kobayashi, Tatsuru Masuda（ Role： Joint author）

Academic Press 2019.04 （ ISBN:978-0-12-819982-4 ）

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Responsible for pages：133-161
Genetic analysis of chloroplast biogenesis, and function and mutant collections

Sho Fujii, Hajime Wada, Koichi Kobayashi（ Role： Joint author）

Humana Press 2018.10

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Responsible for pages：341-365
Roles of lipids in photosynthesis. In Lipids in Plant and Algae Development.

Koichi Kobayashi, Kaichiro Endo, Hajime Wada（ Role： Joint author）

Springer International Publishing 2016.04

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Responsible for pages：21-49
Role of lipids in chloroplast biogenesis. In Lipids in Plant and Algae Development.

Koichi Kobayashi, Hajime Wada（ Role： Joint author）

Springer International Publishing 2016.04

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Responsible for pages：103-125
Regulation of chlorophyll metabolism in plants. In Handbook of Photosynthesis. Third Edition.

Koichi Kobayashi, Tatsuru Masuda（ Role： Joint author）

CRC Press 2016.03

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Responsible for pages：173-192
Spatial and temporal regulation of chloroplast development in Arabidopsis roots. In Photosynthesis Rsearch for Food, Fuel and the Future

Koichi Kobayashi, Tatsuru Masuda（ Role： Joint author）

Springer International Publishing 2013.02

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Responsible for pages：389-393
Tetrapyrrole biosynthesis in plant systems. In Handbook of Porphyrin Science.

Koichi Kobayashi, Tatsuru Masuda（ Role： Joint author）

World Scientific Publishing 2012.08

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Responsible for pages：141-211
Biosynthesis and function of monogalactosyldiacylglycerol, the signature lipid of chloroplasts. In The Chloroplast: Basics and Application.

Yuki Nakamura, Mie Shimojima, Hiroyuki Ohta, Koichi Kobayashi（ Role： Joint author）

Springer 2010.07

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Responsible for pages：185-202
Possible requirement of galactolipids for embryogenesis. In Photosynthesis. Energy from the Sun.

Koichi Kobayashi, Hiroyuki Ohta（ Role： Joint author）

Springer 2008.11

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Responsible for pages：783-786
Histochemical analysis of three MGD genes using promoter-GUS fusion system in Arabidopsis. In Advanced Research on Plant Lipids.

Koichi Kobayashi, Koichiro Awai, Hiroshi Shimada, Tatsuru Masuda, Kenichiro Takamiya, Hiroyuki Ohta（ Role： Joint author）

Kluwer Academic Publishers 2003.02

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Responsible for pages：199-202

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MISC

植物特有の膜脂質が駆動する葉緑体形成 Invited Reviewed International journal

吉原晶子，小林康一

生物物理 64 ( 6 ) 313 - 316 2024.12

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Authorship：Last author,　Corresponding author International / domestic magazine：Domestic journal

DOI： 10.2142/biophys.64.313
エチオプラスト内膜系における酸性脂質の役割—Roles of anionic lipids in etioplast membranes

吉原晶子, 小林啓子, 永田典子, 藤井祥, 和田元, 小林康一

光合成研究 = News letter / The Japanese Society of Photosynthesis Research 34 ( 1 ) 31 - 37 2024.04（ ISSN:1884-2852 ）

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Other URL： https://ndlsearch.ndl.go.jp/books/R000000004-I033550237
高校生物で"葉緑体の外包膜の起源"をどう教えるか : 複数の説が並立する現状と教科書での扱いについて考える

小林康一, 佐藤直樹

遺伝 : 生物の科学 77 ( 1 ) 10 - 17 2023.01（ ISSN:0387-0022 ）
脂質の化学構造が紐解く生理機能の多様性-3 植物の脂質合成の起源と進化色素体が可能にした植物独自の脂質合成経路 Invited Reviewed International journal

小林康一, 川本純

化学と生物 61 ( 8 ) 394 - 401 2023

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Authorship：Lead author,　Corresponding author Publishing type：Article, review, commentary, editorial, etc. (scientific journal) International / domestic magazine：Domestic journal
脂質の化学構造が紐解く生理機能の多様性(2)生体膜脂質のダイナミクスと膜輸送担体 : 膜構造とタンパク質の機能関連

木村泰久, 小林康一

化学と生物 : 日本農芸化学会会誌 : 生命・食・環境 / 日本農芸化学会編 60 ( 9 ) 474 - 480 2022.09（ ISSN:0453-073X ）
Evolutionary history of mycoheterotrophic plants

末次健司, 小林康一

光合成研究 32 ( 1 ) 2022（ ISSN:1884-2852 ）

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J-GLOBAL
Evolution and diversity of chloroplasts from the viewpoint of lipid metabolism Reviewed

BSJ-Review 12 23 - 37 2021.03

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Evolution and diversity of chloroplasts from the viewpoint of lipid metabolism

小林康一, 吉原晶子

植物科学の最前線(Web) 12 2021（ ISSN:2432-9819 ）

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J-GLOBAL
Role of galactolipids in etioplast development and etioplast-to-chloroplast differentiation Invited Reviewed International journal

28 ( 3 ) 139 - 147 2018.12

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Authorship：Last author,　Corresponding author Publishing type：Article, review, commentary, editorial, etc. (scientific journal) International / domestic magazine：Domestic journal

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Presentations

CAM植物においてCO2除去がクロロフィル蛍光に与える影響の解析 Domestic conference

小林康一，戸次咲恵，野上規子，末次健司

第66回日本植物生理学会年会 2025.03

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Presentation type：Oral presentation (general)
酸性脂質PG, SQDGは効率的な葉緑体形成に必要である Domestic conference

吉原晶子，上床理沙，小林啓子，永田典子，小林康一

第66回日本植物生理学会年会 2025.03

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Presentation type：Poster presentation
エチオプラストにおけるホスファチジルグリセロール完全欠損の影響 Domestic conference

川向真翔，吉原晶子，小林啓子，永田典子，藤井祥，小林康一

第66回日本植物生理学会年会 2025.03

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Presentation type：Oral presentation (general)
沖縄の水路に生育するシフォナス緑藻チョウチンミドロ(Dichotomosiphon tuberosus)の光合成活性と色素分布 Domestic conference

関荘一郎，小林康一，藤井律子

第66回日本植物生理学会年会 2025.03

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Presentation type：Oral presentation (general)
Role of thylakoid lipids in the regulation of photosynthesis Invited International conference

Koichi Kobayashi

Gordon Research Conference, Plant Lipids: Structure, Metabolism and Function 2025.01

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Presentation type：Oral presentation (invited, special)

Venue：Pomona, California, United States
Essential roles of phosphatidylglycerol in chloroplast biogenesis Invited International conference

Akiko Yoshihara, Manato Kawamukai, Risa Uwatoko, Keiko Kobayashi, Noriko Nagata, Koichi Kobayashi

2nd Asia-Oceania International Congress on Photosynthesis 2024.09

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Presentation type：Oral presentation (invited, special)

Venue：Kobe, Japan
Thylakoid lipid biosynthesis as a trigger of photosynthesis-associated gene expression and chloroplast biogenesis Invited International conference

Sho Fujii, Akiko Yoshihara, and Koichi Kobayashi

International Symposium on Photosynthesis Research in Honor of Eva-Mari Aro 2024.09

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Presentation type：Oral presentation (invited, special)

Venue：Turku, Finland
Membrane lipids play crucial roles in chlorophyll biosynthesis during chloroplast biogenesis Invited International conference

Akiko Yoshihara, Sho Fujii, Koichi Kobayashi

18th International Congress on Photobiology 2024.08

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Presentation type：Oral presentation (invited, special)

Venue：Perth, Australia
Thylakoid biogenesis and photosynthetic activation driven by plastid lipid biosynthesis Invited International conference

Koichi Kobayashi

26th International Symposium on Plant Lipids 2024.07

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Presentation type：Oral presentation (invited, special)
エチオプラストの発達や機能にホスファチジルグリセロールは必須なのか？

川向真翔、吉原晶子、小林啓子、永田典子、小林康一

第65回日本植物生理学会年会 2024.03

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Presentation type：Poster presentation
シロイヌナズナの冠水時に起こるCO2欠乏に対する光合成の光保護機構

戸次咲惠、大西亜依、和田元、小林康一

第65回日本植物生理学会年会 2024.03

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Presentation type：Oral presentation (general)
葉緑体膜脂質であるDGDGとSQDGの機能的相補関係

倉谷美帆、吉原晶子、小林啓子、永田典子、小林康一

第65回日本植物生理学会年会 2024.03

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Presentation type：Oral presentation (general)
Interplay of lipid classes in thylakoid membrane biogenesis and photosynthetic functioning Invited International conference

Koichi Kobayashi

IRN France-Japan Frontiers in Plant Biology (Kyoto) 2023.10

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Presentation type：Oral presentation (invited, special)
Plastid phospholipid PG and sulfolipid SQDG are required for etioplast development and de-etiolation of Arabidopsis thaliana International conference

Akiko Yoshihara, Keiko Kobayashi, Noriko Nagata, Sho Fujii, Hjime Wada, Koichi Kobayashi

IRN France-Japan Frontiers in Plant Biology (Kyoto) 2023.10

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Presentation type：Poster presentation
Protection of the photosynthetic machinery in response to CO2 limitation in submerged Arabidopsis thaliana seedlings International conference

Sae Bekki, Ai Ohnishi, Hajime Wada and Koichi Kobayashi

IRN France-Japan Frontiers in Plant Biology (Kyoto) 2023.10

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Presentation type：Poster presentation
The sulfolipid SQDG compensates loss of DGDG in photosynthetic membranes International conference

Miho Kuratani, Akiko Yoshihara and Koichi Kobayashi

IRN France-Japan Frontiers in Plant Biology (Kyoto) 2023.10

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Presentation type：Poster presentation
Galactolipid biosynthesis involves in GUN1-mediated regulation of photosynthesis-associated genes

Sho Fujii, Hajime Wada and Koichi Kobayashi

The 15th International Conference on Tetrapyrrole Photoreceptors in Photosynthetic Organism 2023.09

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Presentation type：Poster presentation
Roles of plastid phospholipid PG and sulfolipid SQDG in etioplast development and de-etiolation of Arabidopsis thaliana

Akiko Yoshihara, Keiko Kobayashi, Noriko Nagata, Sho Fujii, Hjime Wada, Koichi Kobayashi

The 15th International Conference on Tetrapyrrole Photoreceptors in Photosynthetic Organism 2023.09

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Presentation type：Poster presentation
色素体の働き方改革～光合成は葉の専売特許ではない～

小林康一

第87回日本植物学会年会 2023.09

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Presentation type：Oral presentation (general)
葉緑体形成における酸性リン脂質PGと酸性糖脂質SQDGの関係

吉原晶子、小林啓子、永田典子、小林康一

第87回日本植物学会年会 2023.09

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Presentation type：Oral presentation (general)
色素体における膜脂質合成と遺伝子発現の協調的制御

藤井祥，野戸康生，黒滝悠志，和田元，小林康一

第35回日本植物脂質シンポジウム 2023.09

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Presentation type：Poster presentation
色素体酸性脂質PG, SQDGはエチオプラスト-葉緑体分化時に重要な役割を担う

吉原晶子、小林啓子、永田典子、小林康一

第35回日本植物脂質シンポジウム 2023.09

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Presentation type：Oral presentation (general)
チラコイド膜の形成過程においてホスファチジルグリセロールが果たす役割

吉原晶子、藤井祥、上床理紗、小林啓子、永田典子、和田元、小林康一

第35回日本植物脂質シンポジウム 2023.09

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Presentation type：Poster presentation
エチオプラスト-葉緑体分化における色素体酸性脂質PG, SQDGの役割

吉原晶子、小林啓子、永田典子、小林康一

第23回日本光生物学協会年会 2023.08

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Presentation type：Poster presentation
葉緑体形成における色素体リン脂質PGの役割の解明

吉原晶子、小林啓子、永田典子、小林康一

第13回日本光合成学会年会 2023.06

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Presentation type：Poster presentation
光合成複合体に含まれる脂質からひも解く、シアノバクテリアと植物との共通性と多様性 Invited Domestic conference

小林康一

2022年度ラン藻ゲノム交流会 2022.07

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Presentation type：Public lecture, seminar, tutorial, course, or other speech

Venue：東京/オンライン
光合成と細胞成長がシアノバクテリアSynechocystis sp. PCC 6803の窒素欠乏時のフィコビリソーム分解に与える影響 Domestic conference

吉原晶子，小林康一

第63回日本植物生理学会年会 2022.03

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Presentation type：Poster presentation

Venue：つくば（オンライン）
シロイヌナズナにおけるエチオプラスト発達時の色素体酸性脂質の役割 Domestic conference

吉原晶子、永田典子、和田元、小林康一

第63回日本植物生理学会年会 2022.03

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Presentation type：Oral presentation (general)

Venue：つくば（オンライン）
植物の発達における色素体酸性脂質の相補的関係 Domestic conference

吉原晶子、永田典子、和田元、小林康一

第33回植物脂質シンポジウム 2021.09

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Presentation type：Poster presentation
Are large budgets essential for modern biological studies? Invited Domestic conference

22nd Young Researchers Seminar of Japanese Society of Photosynthesis Research 2021.05

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Presentation type：Oral presentation (invited, special)
シアノバクテリアSynechocystis sp. PCC 6803において窒素欠乏下の光合成と細胞増殖はフィコビリソーム分解に関与する Domestic conference

吉原晶子，小林康一

第11回日本光合成学会年会・シンポジウム 2021.05 日本光合成学会

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Presentation type：Poster presentation
窒素欠乏時におけるシアノバクテリアSynechocystis sp. PCC 6803の細胞増殖とフィコビリソーム分解の関係 Domestic conference

吉原晶子、小林康一

第62回日本植物生理学会年会 2021.03

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Presentation type：Poster presentation
色素体特有の酸性脂質は植物の非光合成器官の成長にも必須である Domestic conference

吉原晶子、和田元、小林康一

第62回日本植物生理学会年会 2021.03

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Presentation type：Poster presentation
STARTドメインをもつタンパク質がゼニゴケにおいて小胞体に由来する脂肪酸の葉緑体糖脂質への取り込みに関与している Domestic conference

平嶋孝志、神保晴彦、小林康一、和田元

第62回日本植物生理学会年会 2021.03

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Presentation type：Poster presentation
地上部切除により誘導される根細胞での葉緑体の発達には小胞体からの脂質供給が欠かせない Domestic conference

小畑智暉、小林康一、多田隈遼亮、赤坂泰輝、射場厚、祢宜淳太郎

第62回日本植物生理学会年会 2021.03

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Presentation type：Poster presentation
ゼニゴケの葉緑体膜脂質代謝に関与するオルガネラ間脂質輸送タンパク質の解析 Domestic conference

平嶋孝志、神保晴彦、小林康一、和田元

日本植物学会第84回大会 2020.09

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Presentation type：Poster presentation
シロイヌナズナの芽生えの発達において色素体酸性脂質の果たす役割 Domestic conference

吉原晶子、和田元、小林康一

日本植物学会第84回大会 2020.09

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Presentation type：Poster presentation
脂質代謝が印す葉緑体の進化と多様化 Domestic conference

小林康一

日本植物学会第84回大会 2020.09

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Presentation type：Oral presentation (invited, special)
葉緑体包膜脂質輸送体を欠損したシロイヌナズナ変異体gles1を用いた根細胞における葉緑体形成誘導の解析 Domestic conference

小畑智暉、多田隈亮介、小林康一、射場厚、祢宜淳太郎

第61回日本植物生理学会年会 2020.03

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Presentation type：Poster presentation
黄化芽生えのエチオプラスト発達時における酸性膜脂質の役割 Domestic conference

吉原晶子、和田元、小林康一

第61回日本植物生理学会年会 2020.03

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Presentation type：Poster presentation
すべては脂質から始まる？葉緑体の初期発生 Domestic conference

小林康一

第61回日本植物生理学会年会 2020.03

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Presentation type：Oral presentation (invited, special)
Roles of galactolipids in plastid development during skotomorphogenesis to photomorphogenesis International conference

Akiko Yoshihara, Kaichiro Endo, Masato Abe, Hajime Wada, Koichi Kobayashi

8th Asian Symposium on Plant Lipid 2019.11

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Presentation type：Poster presentation
Membrane lipids facilitate plastid differentiation before and after light exposure Invited International conference

Koichi Kobayashi, Sho Fujii, Noriko Nagata, Tatsuru Masuda, Hajime Wada

8th Asian Symposium on Plant Lipid 2019.11

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Presentation type：Poster presentation
葉緑体分化過程におけるガラクト脂質合成と光合成関連遺伝子の発現との関係 Domestic conference

藤井祥、永田典子、増田建、和田元、小林康一

第32回植物脂質シンポジウム 2019.09

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Presentation type：Poster presentation
STARTドメインをもつ脂質輸送タンパク質のゼニゴケにおける機能解析 Domestic conference

平嶋孝志、神保晴彦、小林康一、和田元

第32回植物脂質シンポジウム 2019.09

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Presentation type：Poster presentation
チラコイド膜構築過程における脂質合成 Domestic conference

大沢祐香、小林康一、下嶋美恵、粟井光一郎

第32回植物脂質シンポジウム 2019.09

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Presentation type：Poster presentation
Distinct and overlapped roles of MGDG and DGDG in etioplasts of dark-grown Arabidopsis International conference

Fujii Sho, Nagata Noriko, Masuda Tatsuru, Wada Hajime, Kobayashi Koichi

23rd International Symposium on Plant Lipids 2018

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Presentation type：Poster presentation
Roles of galactolipids in plastid development during skotomorphogenesis to photomorphogenesis

Fujii Sho, Nagata Noriko, Masuda Tatsuru, Wada Hajime, Kobayashi Koichi

Japan-Finland Seminar 2018 2018

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Presentation type：Poster presentation
子葉緑化時のクロロフィル合成と光合成遺伝子発現にガラクト脂質合成が与える影響 Domestic conference

藤井祥、永田典子、増田建、和田元、小林康一

日本植物学会第82回大会 2018

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Presentation type：Poster presentation
ガラクト脂質が駆動するエチオプラストから葉緑体へのダイナミックな分化 Domestic conference

藤井祥、永田典子、増田建、和田元、小林康一

第31回植物脂質シンポジウム 2018

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Presentation type：Poster presentation
Distinct and overlapped roles of MGDG and DGDG in etioplasts of dark-grown Arabidopsis Invited International conference

Sho Fujii, Noriko Nagata, Tatsuru Masuda, Hajime Wada and Koichi Kobayashi

7th Asian Symposium on Plant Lipid 2017

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Presentation type：Poster presentation
根が光合成を行うとき、その制御は葉とどのように違うのか？ Domestic conference

大西　亜依，和田　元，小林　康一

日本植物学会第81回大会 2017

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Presentation type：Poster presentation
Regulatory mechanism of chloroplast development in Arabidopsis roots by a GATA transcription factor GNL Domestic conference

大西亜依、小林康一、和田元

第59回日本植物生理学会年会 2017

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Presentation type：Poster presentation
Specific roles and functional overlaps of thylakoid anionic lipids International conference

Koichi Kobayashi, Kaichiro Endo, Sho Fujii, and Hajime Wada

22nd International Symposium on Plant Lipids 2016

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Presentation type：Poster presentation
Regulation of chloroplast differentiation in Arabidopsis roots in response to shoot removal International conference

Koichi Kobayashi, Ai Ohnishi, Daichi Sasaki, Sho Fujii, Akira Iwase, Keiko Sugimoto, Tatsuru Masuda, and Hajime Wada

CSHA Symposium, Latest Advances in Plant Development & Environmental Response 2016

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Presentation type：Poster presentation
植物細胞における PGの合成経路とその機能 Domestic conference

小林康一、遠藤嘉一郎、藤井祥、Ying-Chen Lin、Chun-Hsien Hung、中村友輝、和田元

第29回植物脂質シンポジウム 2016

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Presentation type：Poster presentation
The leafless mycoheterotrophic orchid Cymbidium macrorhizon performs photosynthesis during fruiting season. Domestic conference

Koichi Kobayashi, Kenji Suetsugu, Hajime Wada

第58回植物生理学会年会 2016

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Presentation type：Poster presentation
シロイヌナズナの根でみる光と植物ホルモンによる光合成の制御 Domestic conference

小林康一、大西亜依、藤井祥、和田元

日本植物学会第79回大会 2015

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Presentation type：Poster presentation
光合成生物におけるPGとSQDGの機能 Domestic conference

小林康一、遠藤嘉一郎、和田元

第28回植物脂質シンポジウム 2015

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Presentation type：Poster presentation
Specific roles and functional overlaps of thylakoid anionic lipids in Arabidopsis Domestic conference

Koichi Kobayashi, Kaichiro Endo, Hajime Wada

第57回日本植物生理学会年会 2015

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Presentation type：Poster presentation
Regulation of chloroplast differentiation in Arabidopsis roots Invited International conference

K Kobayashi, T Masuda, H Wada

Japanese-Finnish Seminar 2014 2014

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Presentation type：Poster presentation
シロイヌナズナの葉緑体バイオジェネシスにおける酸性膜脂質の役割 Domestic conference

小林康一、藤井祥、堀遥香、和田元

日本植物学会第78回大会 2014

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Presentation type：Poster presentation
シロイヌナズナを用いた葉緑体発達制御機構の解明 Invited Domestic conference

小林康一

日本植物学会第78回大会 2014

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Presentation type：Oral presentation (invited, special)
葉緑体機能におけるPGと糖脂質の関係 Domestic conference

小林康一、藤井祥、遠藤嘉一郎、堀遥香、和田元

第27回植物脂質シンポジウム 2014

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Presentation type：Poster presentation
Coordination of galactolipid synthesis with formation of photosynthetic complexes and organelle differentiation International conference

K Kobayashi, S Fujii, Y Nakamura, T Masuda, H Ohta, H Wada

21st International Symposium on Plant Lipids 2014

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Presentation type：Poster presentation

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Outline of collaborative research (seeds)

葉緑体の分化制御機構の解明

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植物がどのような環境やシグナルによって葉緑体機能を発現し光合成をするのかを研究している。

Grant-in-Aid for Scientific Research

プラスチド相転換ダイナミクス

学術変革領域研究(B) 2024
脂質駆動によるチラコイド膜形成過程と葉緑体分化機構の解明

学術変革領域研究(B) 2024

Charge of on-campus class subject

生物学特別研究2A

2024 Intensive lecture Graduate school
生物学特別研究1A

2024 Intensive lecture Graduate school
生体機能生物学演習2A

2024 Intensive lecture Graduate school
生体機能生物学演習1A

2024 Intensive lecture Graduate school
生物学特別研究5A

2024 Intensive lecture Graduate school
生物学特別研究4A

2024 Intensive lecture Graduate school
生物学特別研究3A

2024 Intensive lecture Graduate school
生物学実験A

2024 Weekly class Graduate school
生物学実験A

2024 Weekly class Graduate school
生物学実験A

2024 Weekly class Graduate school
生物学1

2024 Weekly class Graduate school
生物学特別研究2B

2024 Intensive lecture Graduate school
生物学特別研究1B

2024 Intensive lecture Graduate school
海外特別研究2

2024 Intensive lecture Graduate school
海外特別研究1

2024 Intensive lecture Graduate school
生体機能生物学演習2B

2024 Intensive lecture Graduate school
生体機能生物学演習1B

2024 Intensive lecture Graduate school
生体機能生物学特論F

2024 Weekly class Graduate school
生体機能生物学特論F

2024 Weekly class Graduate school
植物の科学

2024 Weekly class Graduate school
生物学特別研究5B

2024 Intensive lecture Graduate school
生物学特別研究4B

2024 Intensive lecture Graduate school
生物学特別研究3B

2024 Intensive lecture Graduate school
海外特別研究5

2024 Intensive lecture Graduate school
海外特別研究4

2024 Intensive lecture Graduate school
海外特別研究3

2024 Intensive lecture Graduate school
生体機能生物学特別演習

2024 Intensive lecture Graduate school
生体機能生物学ゼミナール

2024 Intensive lecture Graduate school
生物学実験B

2024 Weekly class Graduate school
生物学実験A

2024 Weekly class Graduate school
生物学2

2024 Weekly class Graduate school
Fundamental Experiments of Biology

2021 Practical Training
Fundamental Experiments of Biology

2021 Practical Training
Biology B

2021
Biology A

2021
Plant physiology

2021
Biology A

2021
Fundamental Experiments of Biology

2021 Practical Training
Fundamental Experiments of Biology

2021 Practical Training

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