Updated on 2024/04/22

写真a

 
KOBAYASHI Koichi
 
Organization
Faculty of Liberal Arts, Sciences and Global Education Associate Professor
Graduate School of Science Department of Biological Chemistry
Title
Associate Professor
Affiliation
Institute of Liberal Arts, Sciences and Global Education

Position

  • Faculty of Liberal Arts, Sciences and Global Education 

    Associate Professor  2022.04 - Now

  • Graduate School of Science Department of Biology 

    Associate Professor  2022.07 - Now

Degree

  • 博士(理学) ( Tokyo Institute of Technology )

Research Areas

  • Life Science / Plant molecular biology and physiology  / Plant Science

  • Life Science / Plant molecular biology and physiology

Research Interests

  • chloroplast

  • lipid

  • plant

  • photosynthesis

  • chlorophyll

Research subject summary

  • 植物の葉緑体の発達の仕組みとその制御機構

Research Career

  • Regulatory mechanism of chloroplast development

    Chloroplast, Plastid, Plant, Arabidopsis thaliana, Photosynthesis  Individual

    2018 - 2023 

  • Regulation of chlorophyll metabolism

    Chlorophyll, Tetrapyrrole  Individual

    2018 - 2023 

  • Role of membrane lipids in chloroplast

    Phospholipid, Glycolipid, Thylakoid membrane  Individual

    2018 - 2023 

Professional Memberships

  • 日本光合成学会

    2009.05 - Now   Domestic

  • 日本植物学会

    2009.01 - Now   Domestic

  • 日本植物生理学会

    2008.01 - Now   Domestic

  • 日本植物脂質研究会

    2007.04 - Now   Domestic

Awards

  • 文部科学大臣表彰若手科学者賞

    2015.04   文部科学省  

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    Country:Japan

  • 日本植物学会奨励賞

    2014.09   日本植物学会  

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    Country:Japan

Papers

  • Anionic lipids facilitate membrane development and protochlorophyllide biosynthesis in etioplasts.

    Akiko Yoshihara, Keiko Kobayashi, Noriko Nagata, Sho Fujii, Hajime Wada, Koichi Kobayashi

    Plant physiology   194 ( 3 )   1692 - 1704   2024.02

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Dark-germinated angiosperm seedlings develop chloroplast precursors called etioplasts in cotyledon cells. Etioplasts develop lattice membrane structures called prolamellar bodies (PLBs), where the chlorophyll intermediate protochlorophyllide (Pchlide) forms a ternary complex with NADPH and light-dependent NADPH:Pchlide oxidoreductase (LPOR). The lipid bilayers of etioplast membranes are mainly composed of galactolipids, which play important roles in membrane-associated processes in etioplasts. Although etioplast membranes also contain 2 anionic lipids, phosphatidylglycerol (PG) and sulfoquinovosyldiacylglycerol (SQDG), their roles are unknown. To determine the roles of PG and SQDG in etioplast development, we characterized etiolated Arabidopsis (Arabidopsis thaliana) mutants deficient in PG and SQDG biosynthesis. A partial deficiency in PG biosynthesis loosened the lattice structure of PLBs and impaired the insertion of Mg2+ into protoporphyrin IX, leading to a substantial decrease in Pchlide content. Although a complete lack of SQDG biosynthesis did not notably affect PLB formation and Pchlide biosynthesis, lack of SQDG in addition to partial PG deficiency strongly impaired these processes. These results suggested that PG is required for PLB formation and Pchlide biosynthesis, whereas SQDG plays an auxiliary role in these processes. Notably, PG deficiency and lack of SQDG oppositely affected the dynamics of LPOR complexes after photoconversion, suggesting different involvements of PG and SQDG in LPOR complex organization. Our data demonstrate pleiotropic roles of anionic lipids in etioplast development.

    DOI: 10.1093/plphys/kiad604

    PubMed

  • Biosynthesis of phosphatidylglycerol in photosynthetic organisms. Invited Reviewed

    Koichi Kobayashi, Haruhiko Jimbo, Yuki Nakamura, Hajime Wada

    Progress in lipid research   93   101266 - 101266   2024.01

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    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    DOI: 10.1016/j.plipres.2023

  • Evolutionary implications from lipids in membrane bilayers and photosynthetic complexes in cyanobacteria and chloroplasts Invited Reviewed

    Koichi Kobayashi, Akiko Yoshihara, Hisako Kubota-Kawai

    The Journal of Biochemistry   174 ( 5 )   399 - 408   2023.07

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    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    DOI: 10.1093/jb/mvad058

  • Aerial roots of the leafless epiphytic orchid Taeniophyllum are specialized for performing crassulacean acid metabolism photosynthesis.

    Kenji Suetsugu, Ryohei Sugita, Akiko Yoshihara, Hidehito Okada, Kae Akita, Noriko Nagata, Keitaro Tanoi, Koichi Kobayashi

    The New phytologist   238 ( 3 )   932 - 937   2023.02

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    International / domestic magazine:International journal  

    DOI: 10.1111/nph.18812

    PubMed

  • Crucial importance of length of fatty-acyl chains bound to the sn-2 position of phosphatidylglycerol for growth and photosynthesis of Synechocystis sp. PCC 6803 Reviewed

    Kaichiro Endo, Masato Abe, Nobumasa Kawanishi, Haruhiko Jimbo, Koichi Kobayashi, Tomoko Suzuki, Noriko Nagata, Hideto Miyoshi, Hajime Wada

    Biochimica et Biophysica Acta   1867 ( 7 )   159158 - 159158   2022.07( ISSN:1388-1981

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    DOI: 10.1016/j.bbalip.2022.159158

  • Impacts of phosphatidylglycerol on plastid gene expression and light induction of nuclear photosynthetic genes. Reviewed International coauthorship

    Sho Fujii, Koichi Kobayashi, Ying-Chen Lin, Yu-Chi Liu, Yuki Nakamura, Hajime Wada

    Journal of experimental botany   73 ( 9 )   2952 - 2970   2022.05

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Phosphatidylglycerol (PG) is the only major phospholipid in the thylakoid membrane of chloroplasts. PG is essential for photosynthesis, and loss of PG in Arabidopsis thaliana results in severe defects of growth and chloroplast development, with decreased chlorophyll accumulation, impaired thylakoid formation, and down-regulation of photosynthesis-associated genes encoded in nuclear and plastid genomes. However, how the absence of PG affects gene expression and plant growth remains unclear. To elucidate this mechanism, we investigated transcriptional profiles of a PG-deficient Arabidopsis mutant pgp1-2 under various light conditions. Microarray analysis demonstrated that reactive oxygen species (ROS)-responsive genes were up-regulated in pgp1-2. However, ROS production was not enhanced in the mutant even under strong light, indicating limited impacts of photooxidative stress on the defects of pgp1-2. Illumination to dark-adapted pgp1-2 triggered down-regulation of photosynthesis-associated nuclear-encoded genes (PhANGs), while plastid-encoded genes were constantly suppressed. Overexpression of GOLDEN2-LIKE1 (GLK1), a transcription factor gene regulating chloroplast development, in pgp1-2 up-regulated PhANGs but not plastid-encoded genes along with chlorophyll accumulation. Our data suggest a broad impact of PG biosynthesis on nuclear-encoded genes partially via GLK1 and a specific involvement of this lipid in plastid gene expression and plant development.

    DOI: 10.1093/jxb/erac034

    PubMed

  • Lipids in photosynthetic protein complexes in the thylakoid membrane of plants, algae, and cyanobacteria. Invited Reviewed

    Akiko Yoshihara, Koichi Kobayashi

    Journal of experimental botany   73 ( 9 )   2735 - 2750   2022.05

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    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    In the thylakoid membrane of cyanobacteria and chloroplasts, many proteins involved in photosynthesis are associated with or integrated into the fluid bilayer matrix formed by four unique glycerolipid classes, monogalactosyldiacylglycerol, digalactosyldiacylglycerol, sulfoquinovosyldiacylglycerol, and phosphatidylglycerol. Biochemical and molecular genetic studies have revealed that these glycerolipids play essential roles not only in the formation of thylakoid lipid bilayers but also in the assembly and functions of photosynthetic complexes. Moreover, considerable advances in structural biology have identified a number of lipid molecules within the photosynthetic complexes such as PSI and PSII. These data have provided important insights into the association of lipids with protein subunits in photosynthetic complexes and the distribution of lipids in the thylakoid membrane. Here, we summarize recent high-resolution observations of lipid molecules in the structures of photosynthetic complexes from plants, algae, and cyanobacteria, and evaluate the distribution of lipids among photosynthetic protein complexes and thylakoid lipid bilayers. By integrating the structural information into the findings from biochemical and molecular genetic studies, we highlight the conserved and differentiated roles of lipids in the assembly and functions of photosynthetic complexes among plants, algae, and cyanobacteria.

    DOI: 10.1093/jxb/erac017

    PubMed

  • Functional divergence of a pair of Arabidopsis phospho-base methyltransferases, PMT1 and PMT3, conferred by distinct N-terminal sequences Reviewed International coauthorship

    Yu-Chi Liu, Yue-Rong Tan, Chin-Wen Chang, Van C Nguyen, Kazue Kanehara, Koichi Kobayashi, Yuki Nakamura

    The Plant journal   110 ( 4 )   1198 - 1212   2022.05

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    In seed plants, phospho-base N-methyltransferase (PMT) catalyzes a key step in the biosynthesis pathway of phosphatidylcholine (PC), the most abundant phospholipid class. Arabidopsis thaliana possesses three copies of PMT, with PMT1 and PMT3 play a primary role because the pmt1 pmt3 double mutant shows considerably reduced PC content with a pale seedling phenotype. Although the function of PMT1 and PMT3 may be redundant because neither of the parental single mutants showed a similar mutant phenotype, major developmental defects and possible functional divergence of these PMTs underlying the pale pmt1 pmt3 seedling phenotype are unknown. Here, we show the major developmental defect of the pale seedlings in xylem of the hypocotyl with partial impairments in chloroplast development and photosynthetic activity in leaves. Although PMT1 and PMT3 are localized at the endoplasmic reticulum, their tissue-specific expression pattern was distinct in hypocotyls and roots. Intriguingly, the function of PMT3 but not PMT1 requires its characteristic N-terminal sequence in addition to the promoter because truncation of the N-terminal sequence of PMT3 or substitution with PMT1 driven by the PMT3 promoter failed to rescue the pale pmt1 pmt3 seedling phenotype. Thus, PMT3 function requires the N-terminal sequence in addition to its promoter, whereas the PMT1 function is defined by the promoter.

    DOI: 10.1111/tpj.15741

    PubMed

  • Photosynthesis and cell growth trigger degradation of phycobilisomes during nitrogen limitation Reviewed

    Akiko Yoshihara, Koichi Kobayashi

    Plant and Cell Physiology   63 ( 2 )   189 - 199   2021.10

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    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)   Kind of work:Joint Work   International / domestic magazine:International journal  

    Under nitrogen (N)-limited conditions, the non-N2-fixing cyanobacterium Synechocystis sp. PCC 6803 (Synechocystis 6803) actively grows during early stages of starvation by performing photosynthesis but gradually stops the growth and eventually enters dormancy to withstand long-term N limitation. During N limitation, Synechocystis 6803 cells degrade the large light-harvesting antenna complex phycobilisomes (PBSs) presumably to avoid excess light absorption and to reallocate available N to essential functions for growth and survival. These two requirements may be driving forces for PBS degradation during N limitation, but how photosynthesis and cell growth affect PBS degradation remains unclear. To address this question, we examined involvements of photosynthesis and cell growth in PBS degradation during N limitation. Treatment of photosynthesis inhibitors and shading suppressed PBS degradation and caused non-bleaching of cells during N limitation. Limitations of photosynthesis after initial gene responses to N limitation suppressed PBS degradation, implying that photosynthesis affects PBS degradation in a post-translational manner. In addition, limitations of cell growth by inhibition of peptidoglycan and fatty acid biosynthesis, low growth temperature and phosphorous starvation suppressed PBS degradation during N limitation. Because decreased photosynthetic activity led to decreased cell growth, and vice versa, photosynthesis and cell growth would inseparably intertwine each other and affect PBS degradation during N limitation in a complex manner. Our data shed light on the coordination mechanisms among photosynthesis, cell growth and PBS degradation during N limitation.

    DOI: 10.1093/pcp/pcab159

    PubMed

  • Plastid anionic lipids are essential for the development of both photosynthetic and non-photosynthetic organs in Arabidopsis thaliana Reviewed

    Akiko Yoshihara, Noriko Nagata, Hajime Wada, Koichi Kobayashi

    International Journal of Molecular Sciences   22 ( 9 )   4860 - 4860   2021.05

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work   International / domestic magazine:International journal  

    The lipid bilayer matrix of the thylakoid membrane of cyanobacteria and chloroplasts of plants and algae is mainly composed of uncharged galactolipids, but also contains anionic lipids sulfoquinovosyldiacylglycerol (SQDG) and phosphatidylglycerol (PG) as major constituents. The necessity of PG for photosynthesis is evident in all photosynthetic organisms examined to date, whereas the requirement of SQDG varies with species. In plants, although PG and SQDG are also found in non-photosynthetic plastids, their importance for the growth and functions of non-photosynthetic organs remains unclear. In addition, plants synthesize another anionic lipid glucuronosyldiacylglycerol (GlcADG) during phosphorus starvation, but its role in plant cells is not elucidated yet. To understand the functional relationships among PG, SQDG, and GlcADG, we characterized several Arabidopsis thaliana mutants defective in biosynthesis of these lipids. The mutants completely lacking both PG and SQDG biosynthesis in plastids showed developmental defects of roots, hypocotyls, and embryos in addition to leaves, which suggests that these lipids are pleiotropically required for the development of both photosynthetic and non-photosynthetic organs. Furthermore, our analysis revealed that SQDG, but not GlcADG, is essential for complementing the role of PG, particularly in photosynthesis under PG-deficient conditions such as phosphorus starvation.

    DOI: 10.3390/ijms22094860

  • A START domain-containing protein is involved in the incorporation of ER-derived fatty acids into chloroplast glycolipids in Marchantia polymorpha Reviewed

    Takashi Hirashima, Haruhiko Jimbo, Koichi Kobayashi, Hajime Wada

    Biochemical and Biophysical Research Communications   534 ( 1 )   436 - 441   2021.01

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work   International / domestic magazine:International journal  

    The appropriate regulation of thylakoid lipid synthesis is essential for the function of chloroplasts. In plant cells, membrane lipids synthesized in the ER are utilized as a precursor for the synthesis of chloroplast glycolipids. This pathway is thought to be mediated by the transport of glycerolipids synthesized in the ER into chloroplasts. However, we have little knowledge about the proteins involved in the lipid transfer between these organelles in plant cells. Here we show a protein, STAR2, containing the START (Steroidogenic acute regulatory protein-related lipid transfer) domain known to function as a lipid transporter, is involved in the incorporation of ER-derived fatty acids into chloroplast glycolipids in Marchantia polymorpha. We found that STAR2 localizes on the chloroplast envelope membrane as a punctuate structure and is required for the increase of C20 fatty acids, which are synthesized in the ER, in chloroplast glycolipids in response to phosphate deprivation. Our results indicate that STAR2 of M. polymorpha is likely to be involved in the lipid transfer from ER to chloroplast, presumably as a lipid transporter.

    DOI: 10.1016/j.bbrc.2020.11.063

    PubMed

  • The leafless orchid cymbidium macrorhizon performs photosynthesis in pericarp during fruiting season Reviewed

    Koichi Kobayashi, Kenji Suetsugu, Hajime Wada

    Plant and Cell Physiology   62 ( 3 )   472 - 481   2021.01

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    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)   Kind of work:Joint Work   International / domestic magazine:International journal  

    Photosynthesis with highly photoreactive chlorophyll (Chl) provides energy for plant growth but with simultaneous risk of photooxidative damage and photoprotection costs. Although the leafless orchid Cymbidium macrorhizon mostly depends on mycorrhizal fungi for carbon, it accumulates Chl particularly during fruiting and may not be fully mycoheterotrophic. In fact, stable isotopic analysis suggested that the fruiting C. macrorhizon specimens obtain a significant proportion of its carbon demands through photosynthesis. However, actual photosynthetic characteristics of this leafless orchid are unknown. To reveal the functionality of photosynthetic electron transport in C. macrorhizon, we compared its photosynthetic properties with those of its relative mixotrophic orchid C. goeringii and the model plant Arabidopsis thaliana. Compared with C. goeringii and A. thaliana, maximum photochemical efficiency of PSII was substantially low in C. macrorhizon. Chl fluorescence induction kinetics revealed that the electron transport capacity of PSII was limited in C. macrorhizon. Chl fluorescence analysis at 77 K suggested partial energetic disconnection of the light-harvesting antenna from the PSII reaction center in C. macrorhizon. Despite its low PSII photochemical efficiency, C. macrorhizon showed photosynthetic electron-transport activity both in the field and under laboratory conditions. C. macrorhizon developed strong non-photochemical quenching in response to increased light intensity as did C. goeringii, suggesting the functionality of photoprotective systems in this orchid. Moreover, C. macrorhizon fruit developed stomata on the pericarp and showed net O2-evolving activity. Our data demonstrate that C. macrorhizon can perform photosynthetic electron transport in the pericarp, although its contribution to net carbon acquisition may be limited.

    DOI: 10.1093/pcp/pcab006

    PubMed

    Repository URL: http://hdl.handle.net/10466/00017656

  • The Endoplasmic Reticulum Pathway for Membrane Lipid Synthesis Has a Significant Contribution toward Shoot Removal-Induced Root Chloroplast Development in Arabidopsis Reviewed

    Tomoki Obata, Koichi Kobayashi, Ryosuke Tadakuma, Taiki Akasaka, Koh Iba, Juntaro Negi

    Plant and Cell Physiology   62 ( 3 )   494 - 501   2021.01

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work   International / domestic magazine:International journal  

    Chloroplast lipids are synthesized via two distinct pathways: the plastidic pathway and endoplasmic reticulum (ER) pathway. We previously reported that the contribution of the two pathways toward chloroplast development is different between mesophyll cells and guard cells in Arabidopsis leaf tissues, and that the ER pathway plays a major role in guard cell chloroplast development. However, little is known about the contribution of the two pathways toward chloroplast development in other tissue cells, and in this study, we focused on the root cells. Chloroplast development is normally repressed in roots but can be induced when the roots are detached from the shoots (root greening). We found that similar to guard cells, root cells exhibit a higher proportion of glycolipid from the ER pathway. Root greening was repressed in the gles1 mutant, which has a defect in ER-to-plastid lipid transportation via the ER pathway, while normal root greening was observed in the ats1 mutant, whose plastidic pathway is blocked. Lipid analysis revealed that the gles1 mutation caused drastic decrease in the ER-derived glycolipids in roots. Furthermore, the gles1 detached roots showed smaller chloroplasts containing little starch than WT. These results suggest that the ER pathway has a significant contribution toward chloroplast development in the root cells.

    DOI: 10.1093/pcp/pcab009

    PubMed

  • An Artificial Conversion of Roots into Organs with Shoot Stem Characteristics by Inducing Two Transcription Factors.

    Shigeru Hanano, Hajime Tomatsu, Ai Ohnishi, Koichi Kobayashi, Yuki Kondo, Shigeyuki Betsuyaku, Eiji Takita, Yoshiyuki Ogata, Keishi Ozawa, Kunihiro Suda, Tsutomu Hosouchi, Takahiro Nagase, Hideyuki Suzuki, Nozomu Sakurai, Hiroshi Masumoto, Hiroo Fukuda, Daisuke Shibata

    iScience   23 ( 7 )   101332 - 101332   2020.07

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Somatic plant cells can regenerate shoots and/or roots or adventitious embryonic calluses, which may induce organ formation under certain conditions. Such regenerations occur via dedifferentiation of somatic cells, induction of organs, and their subsequent outgrowth. Despite recent advances in understanding of plant regeneration, many details of shoot induction remain unclear. Here, we artificially induced shoot stem-like green organs (SSOs) in Arabidopsis thaliana roots via simultaneous induction of two transcription factors (TFs), ARABIDOPSIS THALIANA HOMEOBOX PROTEIN 25 (ATHB25, At5g65410) and the B3 family transcription factor REPRODUCTIVE MERISTEM 7 (REM7, At3g18960). The SSOs exhibited negative gravitropism and differentiated vascular bundle phenotypes. The ATHB25/REM7 induced the expression of genes controlling shoot stem characteristics by ectopic expression in roots. Intriguingly, the restoration of root growth was seen in the consecutive and adjacent parts of the SSOs under gene induction conditions. Our findings thus provide insights into the development and regeneration of plant shoot stems.

    DOI: 10.1016/j.isci.2020.101332

    PubMed

  • Relationship Between Glycerolipids and Photosynthetic Components During Recovery of Thylakoid Membranes From Nitrogen Starvation-Induced Attenuation in Synechocystis sp. PCC 6803.

    Koichi Kobayashi, Yuka Osawa, Akiko Yoshihara, Mie Shimojima, Koichiro Awai

    Frontiers in plant science   11   432 - 432   2020

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Thylakoid membranes, the site of photochemical and electron transport reactions of oxygenic photosynthesis, are composed of a myriad of proteins, cofactors including pigments, and glycerolipids. In the non-diazotrophic cyanobacterium Synechocystis sp. PCC 6803, the size and function of thylakoid membranes are reduced under nitrogen (N) starvation but are quickly recovered after N addition to the starved cells. To understand how the functionality of thylakoid membranes is adjusted in response to N status in Synechocystis sp. PCC 6803, we examined changes in thylakoid components and the photosynthetic activity during the N starvation and recovery processes. In N-starved cells, phycobilisome content, photosystem II protein levels and the photosynthetic activity substantially decreased as compared with those in N-sufficient cells. Although the content of chlorophyll (Chl) a, total protein and total glycerolipid also decreased under the N-starved condition based on OD730 reflecting cell density, when based on culture volume, the Chl a and total protein content remained almost constant and total glycerolipid content even increased during N starvation, suggesting that cellular levels of these components decrease under the N-starved condition mainly through dilution due to cell growth. With N addition, the photosynthetic activity quickly recovered, followed by full restoration of photosynthetic pigment and protein levels. The content of phosphatidylglycerol (PG), an essential lipid constituent of both photosystems, increased faster than that of Chl a, whereas the content of glycolipids, the main constituents of the thylakoid lipid bilayer, gradually recovered after N addition. The data indicate differential regulation of PG and glycolipids during the construction of the photosynthetic machinery and regeneration of thylakoid membranes. Of note, addition of PG to the growth medium slightly accelerated the Chl a accumulation in wild-type cells during the recovery process. Because PG is required for the biosynthesis of Chl a and the formation of functional photosystem complexes, rapid PG biosynthesis in response to N acquisition may be required for the rapid formation of the photosynthetic machinery during thylakoid regeneration.

    DOI: 10.3389/fpls.2020.00432

    PubMed

  • Role of Galactolipids in Plastid Differentiation Before and After Light Exposure. Reviewed

    Fujii S, Wada H, Kobayashi K

    Plants (Basel, Switzerland)   8 ( 10 )   2019.09

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Galactolipids, monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG), are the predominant lipid classes in the thylakoid membrane of chloroplasts. These lipids are also major constituents of internal membrane structures called prolamellar bodies (PLBs) and prothylakoids (PTs) in etioplasts, which develop in the cotyledon cells of dark-grown angiosperms. Analysis of Arabidopsis mutants defective in the major galactolipid biosynthesis pathway revealed that MGDG and DGDG are similarly and, in part, differently required for membrane-associated processes such as the organization of PLBs and PTs and the formation of pigment-protein complexes in etioplasts. After light exposure, PLBs and PTs in etioplasts are transformed into the thylakoid membrane, resulting in chloroplast biogenesis. During the etioplast-to-chloroplast differentiation, galactolipids facilitate thylakoid membrane biogenesis from PLBs and PTs and play crucial roles in chlorophyll biosynthesis and accumulation of light-harvesting proteins. These recent findings shed light on the roles of galactolipids as key facilitators of several membrane-associated processes during the development of the internal membrane systems in plant plastids.

    DOI: 10.3390/plants8100357

    PubMed

  • Galactolipids are essential for internal membrane transformation during etioplast-to-chloroplast differentiation. Reviewed

    Fujii S, Nagata N, Masuda T, Wada H, Kobayashi K

    Plant & cell physiology   60 ( 6 )   1224 - 1238   2019.03( ISSN:0032-0781

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:Domestic journal  

    Etioplasts developed in angiosperm cotyledon cells in darkness rapidly differentiate into chloroplasts with illumination. This process involves dynamic transformation of internal membrane structures from the prolamellar bodies (PLBs) and prothylakoids (PTs) in etioplasts to thylakoid membranes in chloroplasts. Although two galactolipids, monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG), are predominant lipid constituents of membranes in both etioplasts and chloroplasts, their roles in the structural and functional transformation of internal membranes during etioplast-to-chloroplast differentiation are unknown. We previously reported that a 36% loss of MGDG by an artificial microRNA targeting major MGDG synthase (amiR-MGD1) only slightly affected PLB structures but strongly impaired PT formation and protochlorophyllide biosynthesis. Meanwhile, strong DGDG deficiency in a DGDG synthase mutant (dgd1) disordered the PLB lattice structure in addition to impaired PT development and protochlorophyllide biosynthesis. In this study, thylakoid biogenesis after PLB disassembly with illumination was strongly perturbed by amiR-MGD1. The amiR-MGD1 expression impaired the accumulation of Chl and the major light-harvesting complex II protein (LHCB1), which may inhibit rapid transformation from disassembled PLBs to the thylakoid membrane. As did amiR-MGD1 expression, dgd1 mutation impaired the accumulation of Chl and LHCB1 during etioplast-to-chloroplast differentiation. Furthermore, unlike in amiR-MGD1 seedlings, in dgd1 seedlings, disassembly of PLBs after illumination was retarded. Because DGDG but not MGDG prefers to form the bilayer lipid phase in membranes, the MGDG-to-DGDG ratio may strongly affect the transformation of PLBs to the thylakoid membrane during etioplast-to-chloroplast differentiation.

    DOI: 10.1093/pcp/pcz041

    PubMed

  • Site-directed mutagenesis of two amino acid residues in cytochrome b 559 α subunit that interact with a phosphatidylglycerol molecule (PG772) induces quinone-dependent inhibition of photosystem II activity Reviewed

    Kaichiro Endo, Koichi Kobayashi, Hsing-Ting Wang, Hsiu-An Chu, Jian-Ren Shen, Hajime Wada

    Photosynthesis research 雑誌   139   267 - 279   2019.01

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    Kind of work:Joint Work  

  • Blue Light Regulates Phosphate Deficiency-Dependent Primary Root Growth Inhibition in Arabidopsis. Reviewed

    Chuan-Ming Yeh, Koichi Kobayashi, Sho Fujii, Hidehiro Fukaki, Nobutaka Mitsuda, Masaru Ohme-Takagi

    Frontiers in plant science   10   1803 - 1803   2019

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Plants have evolved mechanisms to improve utilization efficiency or acquisition of inorganic phosphate (Pi) in response to Pi deficiency, such as altering root architecture, secreting acid phosphatases, and activating the expression of genes related to Pi uptake and recycling. Although many genes responsive to Pi starvation have been identified, transcription factors that affect tolerance to Pi deficiency have not been well characterized. We show here that the ectopic expression of B-BOX32 (BBX32) and the mutation of ELONGATED HYPOCOTYL 5 (HY5), whose transcriptional activity is negatively regulated by BBX32, resulted in the tolerance to Pi deficiency in Arabidopsis. The primary root lengths of 35S:BBX32 and hy5 plants were only slightly inhibited under Pi deficient condition and the fresh weights were significantly higher than those of wild type. The Pi deficiency-tolerant root phenotype of hy5 was similarly observed when grown on the medium without Pi. In addition, a double mutant, hy5 slr1, without lateral roots, also showed a long primary root phenotype under phosphate deficiency, indicating that the root phenotype of hy5 does not result from an increase of external Pi uptake. Moreover, we found that blue light may regulate Pi deficiency-dependent primary root growth inhibition through activating peroxidase gene expression, suggesting the Pi-deficiency tolerant root phenotype of hy5 may be due to blockage of blue light responses. Altogether, this study points out light quality may play an important role in the regulation of Pi deficiency responses. It may contribute to regulate plant growth under Pi deficiency through proper illumination.

    DOI: 10.3389/fpls.2019.01803

    PubMed

  • A Novel Conversion of Roots into Organs with Shoot Stem Characteristics by Inducing Two Transcription Factors

    Shigeru Hanano, Hajime Tomatsu, Ai Ohnishi, Koichi Kobayashi, Yuki Kondo, Shigeyuki Betsuyaku, Eiji Takita, Yoshiyuki Ogata, Keishi Ozawa, Kunihiro Suda, Tsutomu Hosouchi, Takahiro Nagase, Hideyuki Suzuki, Nozomu Sakurai, Hiroo Fukuda, Daisuke Shibata

    SSRN Electronic Journal   2019

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.2139/ssrn.3387683

  • Role of galactolipids in etioplast development and etioplast-to-chloroplast differentiation Reviewed

    28   139 - 147   2018.12

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    Kind of work:Joint Work  

  • High myristic acid content in the cyanobacterium Cyanothece sp. PCC 8801 results from substrate specificity of lysophosphatidic acid Reviewed

    Masakazu Saito, Kaichiro Endo, Koichi Kobayashi, Mai Watanabe, Masahiko Ikeuchi, Akio Murakami, Norio Murata, Hajime Wada

    Biochimica et Biophysica Acta 雑誌   1863   939 - 947   2018.09

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    Kind of work:Joint Work  

  • Thylakoid membrane lipid sulfoquinovosyl-diacylglycerol (SQDG) is required for full functioning of photosystem II in Thermosynechococcus elongatus Reviewed

    Yoshiki Nakajima, Yasufumi Umena, Ryo Nagao, Kaichiro Endo, Koichi Kobayashi, Fusamichi Akita, Michihiro Suga, Hajime Wada, Takumi Noguchi, Jian-Ren Shen

    Journal of Biological Chemistry 雑誌   293   14786 - 14797   2018.09

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    Kind of work:Joint Work  

  • Digalactosyldiacylglycerol is essential for organization of the membrane structure in etioplasts Reviewed

    Sho Fujii, Koichi Kobayashi, Noriko Nagata, Tatsuru Masuda, Hajime Wada

    Plant physiology 雑誌   177   1487 - 1497   2018.08

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    Kind of work:Joint Work  

  • Phosphatidylglycerophosphate phosphatase is required for root growth in Arabidopsis Reviewed

    Ying-Chen Lin, Koichi Kobayashi, Hajime Wada, Yuki Nakamura

    Biochimica et Biophysica Acta 雑誌   1863   563 - 575   2018.06

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    Kind of work:Joint Work  

  • Improved photosynthesis in Arabidopsis roots by activation of GATA transcription factors Reviewed

    Ai Ohnishi, Hajime Wada, Koichi Kobayashi

    Photosynthetica 雑誌   56   433 - 444   2018.03

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    Kind of work:Joint Work  

  • Specific Distribution of Phosphatidylglycerol to Photosystem Complexes in the Thylakoid Membrane Reviewed

    Koichi Kobayashi, Kaichiro Endo, Hajime Wada

    Frontiers in plant science 雑誌   8   2017.11

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    Kind of work:Joint Work  

  • Monogalactosyldiacylglycerol facilitates synthesis of photoactive protochlorophyllide in etioplasts Reviewed

    Sho Fujii, Koichi Kobayashi, Noriko Nagata, Tatsuru Masuda, Hajime Wada

    Plant physiology 雑誌   174   2183 - 2198   2017.08

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    Kind of work:Joint Work  

  • Simultaneous but spatially different regulation of non-photosynthetic callus formation and photosynthetic root development after shoot removal Reviewed

    Koichi Kobayashi, Akira Iwase

    Plant signaling & behavior 雑誌   12   2340 - 2355   2017.06

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    Kind of work:Joint Work  

  • Shoot removal induces chloroplast development in roots via cytokinin signaling Reviewed

    Koichi Kobayashi, Ai Ohnishi, Daichi Sasaki, Sho Fujii, Akira Iwase, Keiko Sugimoto, Tatsuru Masuda, Hajime Wada

    Plant physiology 雑誌   173   2340 - 2355   2017.04

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    Kind of work:Joint Work  

  • Transcriptional regulation of tetrapyrrole biosynthesis in Arabidopsis thaliana Reviewed

    Koichi Kobayashi, Tatsuru Masuda

    Frontiers in plant science 雑誌   7   2016.12

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    Kind of work:Joint Work  

  • Arabidopsis phosphatidylglycerophosphate phosphatase 1 involved in phosphatidylglycerol biosynthesis and photosynthetic function Reviewed

    Ying‐Chen Lin, Koichi Kobayashi, Chun‐Hsien Hung, Hajime Wada, Yuki Nakamura

    The Plant Journal 雑誌   88   1022 - 1037   2016.12

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    Kind of work:Joint Work  

  • CyanoBase: a large-scale update on its 20th anniversary Reviewed

    Takatomo Fujisawa, Rei Narikawa, Shin-ichi Maeda, Satoru Watanabe, Yu Kanesaki, Koichi Kobayashi, Jiro Nomata, Mitsumasa Hanaoka, Mai Watanabe, Shigeki Ehira, Eiji Suzuki, Koichiro Awai, Yasukazu Nakamura

    Nucleic acids research 雑誌   45   D551 - D554   2016.11

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    Kind of work:Joint Work  

  • Sulfoquinovosyldiacylglycerol has an essential role in Thermosynechococcus elongatus BP-1 under phosphate-deficient conditions Reviewed

    Kaichiro Endo, Koichi Kobayashi, Hajime Wada

    Plant and Cell Physiology 雑誌   57   2461 - 2471   2016.09

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    Kind of work:Joint Work  

  • Allocation of heme is differentially regulated by ferrochelatase isoforms in Arabidopsis cells Reviewed

    Nino A Espinas, Koichi Kobayashi, Yasushi Sato, Nobuyoshi Mochizuki, Kaori Takahashi, Ryouichi Tanaka, Tatsuru Masuda

    Frontiers in plant science 雑誌   7   2016.08

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    Kind of work:Joint Work  

  • Role of membrane glycerolipids in photosynthesis, thylakoid biogenesis and chloroplast development Reviewed

    Koichi Kobayashi

    Journal of plant research 雑誌   129   565 - 580   2016.07

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    Kind of work:Single Work  

  • Multiple impacts of loss of plastidic phosphatidylglycerol biosynthesis on photosynthesis during seedling growth of Arabidopsis Reviewed

    Koichi Kobayashi, Kaichiro Endo, Hajime Wada

    Frontiers in plant science 雑誌   7   2016.03

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    Kind of work:Joint Work  

  • Functional specificity of cardiolipin synthase revealed by the identification of a cardiolipin synthase CrCLS1 in Chlamydomonas reinhardtii Reviewed

    Chun-Hsien Hung, Koichi Kobayashi, Hajime Wada, Yuki Nakamura

    Frontiers in microbiology 雑誌   6   2016.01

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    Kind of work:Joint Work  

  • Site-directed mutagenesis of amino acid residues of D1 protein interacting with phosphatidylglycerol affects the function of plastoquinone QB in photosystem II Reviewed

    Kaichiro Endo, Naoki Mizusawa, Jian-Ren Shen, Masato Yamada, Tatsuya Tomo, Hirohisa Komatsu, Masami Kobayashi, Koichi Kobayashi, Hajime Wada

    Photosynthesis research 雑誌   126   385 - 397   2015.12

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    Kind of work:Joint Work  

  • Biosynthesis of Thylakoid Membrane Lipids in Plants and Their Roles in Chloroplast Biogenesis Reviewed

    25   126 - 137   2015.08

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    Kind of work:Single Work  

  • Characterization of Chlamydomonas reinhardtii phosphatidylglycerophosphate synthase in Synechocystis sp. PCC 6803 Reviewed

    Chun-Hsien Hung, Kaichiro Endo, Koichi Kobayashi, Yuki Nakamura, Hajime Wada

    Frontiers in microbiology 雑誌   6   2015.08

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    Kind of work:Joint Work  

  • Lipids in Photosynthetic Protein Complexes Reviewed

    25   116 - 125   2015.08

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    Kind of work:Joint Work  

  • Isolation and characterization of a phosphatidylglycerophosphate phosphatase1, PGPP1, in Chlamydomonas reinhardtii Reviewed

    Chun-Hsien Hung, Koichi Kobayashi, Hajime Wada, Yuki Nakamura

    Plant Physiology and Biochemistry 雑誌   92   56 - 61   2015.07

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    Kind of work:Joint Work  

  • Specific role of phosphatidylglycerol and functional overlaps with other thylakoid lipids in Arabidopsis chloroplast biogenesis Reviewed

    Koichi Kobayashi, Sho Fujii, Mayuko Sato, Kiminori Toyooka, Hajime Wada

    Plant cell reports 雑誌   2015.04

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    Kind of work:Joint Work  

  • Expression Analysis of Transcription Factors Involved in Chloroplast Differentiation Reviewed

    Dwi Andi Listiawan, Ryo Tanoue, Koichi Kobayashi, Tatsuru Masuda

    Procedia Chemistry 雑誌   14   146 - 151   2015.01

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    Kind of work:Joint Work  

  • Inducible knockdown of MONOGALACTOSYLDIACYLGLYCEROL SYNTHASE1 reveals roles of galactolipids in organelle differentiation in Arabidopsis cotyledons Reviewed

    Sho Fujii, Koichi Kobayashi, Yuki Nakamura, Hajime Wada

    Plant physiology 雑誌   166   1436 - 1449   2014.11

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    Kind of work:Joint Work  

  • Molecular phylogeny and intricate evolutionary history of the three isofunctional enzymes involved in the oxidation of protoporphyrinogen IX Reviewed

    Koichi Kobayashi, Tatsuru Masuda, Naoyuki Tajima, Hajime Wada, Naoki Sato

    Genome biology and evolution 雑誌   6   2141 - 2155   2014.08

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    Kind of work:Joint Work  

  • Monogalactosyldiacylglycerol synthesis in the outer envelope membrane of chloroplasts is required for enhanced growth under sucrose supplementation Reviewed

    Masato Murakawa, Mie Shimojima, Yuichi Shimomura, Koichi Kobayashi, Koichiro Awai, Hiroyuki Ohta

    Frontiers in Plant Science 雑誌   5   2014.06

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    Kind of work:Joint Work  

  • Transcriptional regulation of thylakoid galactolipid biosynthesis coordinated with chlorophyll biosynthesis during the development of chloroplasts in Arabidopsis Reviewed

    Koichi Kobayashi, Sho Fujii, Daichi Sasaki, Shinsuke Baba, Hiroyuki Ohta, Tatsuru Masuda, Hajime Wada

    Frontiers in Plant Science 雑誌   5   2014.06

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    Kind of work:Joint Work  

  • Klebsormidium flaccidum genome reveals primary factors for plant terrestrial adaptation Reviewed

    Koichi Hori, Fumito Maruyama, Takatomo Fujisawa, Tomoaki Togashi, Nozomi Yamamoto, Mitsunori Seo, Syusei Sato, Takuji Yamada, Hiroshi Mori, Naoyuki Tajima, Takashi Moriyama, Masahiko Ikeuchi, Mai Watanabe, Hajime Wada, Koichi Kobayashi, Masakazu Saito, Tatsuru Masuda, Yuko Sasaki-Sekimoto, Kiyoshi Mashiguchi, Koichiro Awai, Mie Shimojima, Shinji Masuda, Masako Iwai, Takashi Nobusawa, Takafumi Narise, Satoshi Kondo, Hikaru Saito, Ryoichi Sato, Masato Murakawa, Yuta Ihara, Yui Oshima-Yamada, Kinuka Ohtaka, Masanori Satoh, Kohei Sonobe, Midori Ishii, Ryosuke Ohtani, Miyu Kanamori-Sato, Rina Honoki, Daichi Miyazaki, Hitoshi Mochizuki, Jumpei Umetsu, Kouichi Higashi, Daisuke Shibata, Yuji Kamiya, Naoki Sato, Yasukazu Nakamura, Satoshi Tabata, Shigeru Ida, Ken Kurokawa, Hiroyuki Ohta

    Nature communications 雑誌   5   2014.05

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    Kind of work:Joint Work  

  • Photosynthesis of root chloroplasts developed in Arabidopsis lines overexpressing GOLDEN2-LIKE transcription factors

    Koichi Kobayashi, Daichi Sasaki, Ko Noguchi, Daiki Fujinuma, Hirohisa Komatsu, Masami Kobayashi, Mayuko Sato, Kiminori Toyooka, Keiko Sugimoto, Krishna K Niyogi, Hajime Wada, Tatsuru Masuda

    Plant and cell physiology   54   1365 - 1377   2013.06

  • Role of galactolipid biosynthesis in coordinated development of photosynthetic complexes and thylakoid membranes during chloroplast biogenesis in Arabidopsis Reviewed

    Koichi Kobayashi, Takafumi Narise, Kintake Sonoike, Haruki Hashimoto, Naoki Sato, Maki Kondo, Mikio Nishimura, Mayuko Sato, Kiminori Toyooka, Keiko Sugimoto, Hajime Wada, Tatsuru Masuda, Hiroyuki Ohta

    The Plant Journal 雑誌   73   250 - 261   2013.01

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    Kind of work:Joint Work  

  • Regulation of Chlorophyll Biosynthesis in Higher Plants Reviewed

    22   125 - 138   2012.08

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    Kind of work:Single Work  

  • Role of the G-box element in regulation of chlorophyll biosynthesis in arabidopsis roots Reviewed

    Koichi Kobayashi, Takeshi Obayashi, Tatsuru Masuda

    Plant Signaling & Behavior 雑誌   7   11 - 10   2012.08

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    Kind of work:Joint Work  

  • Evaluation of Unbound Free Heme in Plant Cells by Differential Acetone Extraction Reviewed

    Nino A Espinas, Koichi Kobayashi, Shigekazu Takahashi, Nobuyoshi Mochizuki, Tatsuru Masuda

    Plant and Cell Physiology 雑誌   53   1344 - 1354   2012.07

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    Kind of work:Joint Work  

  • Regulation of root greening by light and auxin/cytokinin signaling in Arabidopsis Reviewed

    Koichi Kobayashi, Shinsuke Baba, Takeshi Obayashi, Mayuko Sato, Kiminori Toyooka, Mika Keränen, Eva-Mari Aro, Hidehiro Fukaki, Hiroyuki Ohta, Keiko Sugimoto, Tatsuru Masuda

    The Plant Cell 雑誌   24   1081 - 1095   2012.03

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    Kind of work:Joint Work  

  • Deficiency in riboflavin biosynthesis affects tetrapyrrole biosynthesis in etiolated Arabidopsis tissue Reviewed

    Boris Hedtke, Ali Alawady, Alfonso Albacete, Koichi Kobayashi, Michael Melzer, Thomas Roitsch, Tatsuru Masuda, Bernhard Grimm

    Plant molecular biology 雑誌   78   77 - 93   2012.01

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    Kind of work:Joint Work  

  • Tetrapyrrole metabolism in Arabidopsis thaliana Reviewed

    Ryouichi Tanaka, Koichi Kobayashi, Tatsuru Masuda

    The Arabidopsis book 著書   9   2011.07

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    Kind of work:Joint Work  

  • Involvement of auxin signaling mediated by IAA14 and ARF7/19 in membrane lipid remodeling during phosphate starvation Reviewed

    Takafumi Narise, Koichi Kobayashi, Shinsuke Baba, Mie Shimojima, Shinji Masuda, Hidehiro Fukaki, Hiroyuki Ohta

    Plant molecular biology 雑誌   72   533 - 544   2010.03

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    Kind of work:Joint Work  

  • Galactolipid Synthesis and Its Roles in Higher Plants Reviewed

    19   52 - 58   2009.08

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  • Activation of galactolipid biosynthesis in development of pistils and pollen tubes Reviewed

    Yuki Nakamura, Koichi Kobayashi, Hiroyuki Ohta

    Plant Physiology and Biochemistry 雑誌   47   535 - 539   2009.06

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    Kind of work:Joint Work  

  • Type A and type B monogalactosyldiacylglycerol synthases are spatially and functionally separated in the plastids of higher plants

    Koichi Kobayashi, Yuki Nakamura, Hiroyuki Ohta

    Plant Physiology and Biochemistry 雑誌   47   518 - 525   2009.06

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    Kind of work:Joint Work  

  • Galactolipid synthesis and its role in membrane biogenesis in higher plants Reviewed

    21   29 - 39   2009.04

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    Kind of work:Joint Work  

  • Type‐B monogalactosyldiacylglycerol synthases are involved in phosphate starvation‐induced lipid remodeling, and are crucial for low‐phosphate adaptation Reviewed

    Koichi Kobayashi, Koichiro Awai, Masanobu Nakamura, Akira Nagatani, Tatsuru Masuda, Hiroyuki Ohta

    The Plant Journal 雑誌   57   322 - 331   2009.01

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    Kind of work:Joint Work  

  • Functional analysis of Arabidopsis thaliana isoforms of the Mg-chelatase CHLI subunit Reviewed

    Koichi Kobayashi, Nobuyoshi Mochizuki, Naho Yoshimura, Ken Motohashi, Toru Hisabori, Tatsuru Masuda

    Photochemical & Photobiological Sciences 雑誌   7   1188 - 1195   2008.06

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    Kind of work:Joint Work  

  • 葉緑体チラコイド膜を構成するグリセロ糖脂質の生合成と機能

    下嶋美恵, 小林康一, 太田啓之

    化学と生物 雑誌   46   330 - 338   2008.05

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    Kind of work:Joint Work  

  • Galactolipid synthesis in chloroplast inner envelope is essential for proper thylakoid biogenesis, photosynthesis, and embryogenesis Reviewed

    Koichi Kobayashi, Maki Kondo, Hiroaki Fukuda, Mikio Nishimura, Hiroyuki Ohta

    Proceedings of the National Academy of Sciences of USA 雑誌   2007.10

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    Kind of work:Joint Work  

  • Membrane lipid alteration during phosphate starvation is regulated by phosphate signaling and auxin/cytokinin cross‐talk Reviewed

    Koichi Kobayashi, Tatsuru Masuda, Ken‐ichiro Takamiya, Hiroyuki Ohta

    The Plant Journal 雑誌   47   238 - 248   2006.07

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    Kind of work:Joint Work  

  • 12-oxo-phytodienoic acid triggers expression of a distinct set of genes and plays a role in wound-induced gene expression in Arabidopsis Reviewed

    Nozomi Taki, Yuko Sasaki-Sekimoto, Takeshi Obayashi, Akihiro Kikuta, Koichi Kobayashi, Takayuki Ainai, Kaori Yagi, Nozomu Sakurai, Hideyuki Suzuki, Tatsuru Masuda, Ken-ichiro Takamiya, Daisuke Shibata, Yuichi Kobayashi, Hiroyuki Ohta

    Plant physiology 雑誌   139   1268 - 1283   2005.11

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  • リン酸欠乏時における膜脂質合成系の代謝変動と植物ホルモンによる制御

    小林 康一, 太田 啓之

    日本農芸化学会誌 雑誌   78   977 - 980   2004.10

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    Kind of work:Joint Work  

  • Arabidopsis type B monogalactosyldiacylglycerol synthase genes are expressed during pollen tube growth and induced by phosphate starvation Reviewed

    Koichi Kobayashi, Koichiro Awai, Ken-ichiro Takamiya, Hiroyuki Ohta

    Plant physiology 雑誌   134   640 - 648   2004.02

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    Kind of work:Joint Work  

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Books and Other Publications

  • 光合成

    高橋 裕一郎,園池 公毅,古本 強 他( Role: Joint author)

    朝倉書店  2021.12  ( ISBN:ISBN978-4-254-17176-

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    Responsible for pages:16-19  

  • Transcriptional control for the chlorophyll metabolism

    Koichi Kobayashi, Tatsuru Masuda( Role: Joint author)

    Academic Press  2019.04  ( ISBN:978-0-12-819982-4

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    Responsible for pages:133-161  

  • Genetic analysis of chloroplast biogenesis, and function and mutant collections

    Sho Fujii, Hajime Wada, Koichi Kobayashi( Role: Joint author)

    Humana Press  2018.10 

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    Responsible for pages:341-365  

  • Roles of lipids in photosynthesis. In Lipids in Plant and Algae Development.

    Koichi Kobayashi, Kaichiro Endo, Hajime Wada( Role: Joint author)

    Springer International Publishing  2016.04 

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    Responsible for pages:21-49  

  • Role of lipids in chloroplast biogenesis. In Lipids in Plant and Algae Development.

    Koichi Kobayashi, Hajime Wada( Role: Joint author)

    Springer International Publishing  2016.04 

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    Responsible for pages:103-125  

  • Regulation of chlorophyll metabolism in plants. In Handbook of Photosynthesis. Third Edition.

    Koichi Kobayashi, Tatsuru Masuda( Role: Joint author)

    CRC Press  2016.03 

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    Responsible for pages:173-192  

  • Spatial and temporal regulation of chloroplast development in Arabidopsis roots. In Photosynthesis Rsearch for Food, Fuel and the Future

    Koichi Kobayashi, Tatsuru Masuda( Role: Joint author)

    Springer International Publishing  2013.02 

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    Responsible for pages:389-393  

  • Tetrapyrrole biosynthesis in plant systems. In Handbook of Porphyrin Science.

    Koichi Kobayashi, Tatsuru Masuda( Role: Joint author)

    World Scientific Publishing  2012.08 

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    Responsible for pages:141-211  

  • Biosynthesis and function of monogalactosyldiacylglycerol, the signature lipid of chloroplasts. In The Chloroplast: Basics and Application.

    Yuki Nakamura, Mie Shimojima, Hiroyuki Ohta, Koichi Kobayashi( Role: Joint author)

    Springer  2010.07 

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    Responsible for pages:185-202  

  • Possible requirement of galactolipids for embryogenesis. In Photosynthesis. Energy from the Sun.

    Koichi Kobayashi, Hiroyuki Ohta( Role: Joint author)

    Springer  2008.11 

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    Responsible for pages:783-786  

  • Histochemical analysis of three MGD genes using promoter-GUS fusion system in Arabidopsis. In Advanced Research on Plant Lipids.

    Koichi Kobayashi, Koichiro Awai, Hiroshi Shimada, Tatsuru Masuda, Kenichiro Takamiya, Hiroyuki Ohta( Role: Joint author)

    Kluwer Academic Publishers  2003.02 

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    Responsible for pages:199-202  

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MISC

  • 高校生物で"葉緑体の外包膜の起源"をどう教えるか : 複数の説が並立する現状と教科書での扱いについて考える

    小林 康一, 佐藤 直樹

    遺伝 : 生物の科学   77 ( 1 )   10 - 17   2023.01( ISSN:0387-0022

  • 脂質の化学構造が紐解く生理機能の多様性-3 植物の脂質合成の起源と進化 色素体が可能にした植物独自の脂質合成経路 Invited Reviewed International journal

    小林康一, 川本純

    化学と生物   61 ( 8 )   394 - 401   2023

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    Authorship:Lead author, Corresponding author   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   International / domestic magazine:Domestic journal  

  • 脂質の化学構造が紐解く生理機能の多様性(2)生体膜脂質のダイナミクスと膜輸送担体 : 膜構造とタンパク質の機能関連

    木村 泰久, 小林 康一

    化学と生物 : 日本農芸化学会会誌 : 生命・食・環境 / 日本農芸化学会 編   60 ( 9 )   474 - 480   2022.09( ISSN:0453-073X

  • Evolutionary history of mycoheterotrophic plants

    末次健司, 小林康一

    光合成研究   32 ( 1 )   2022( ISSN:1884-2852

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  • Evolution and diversity of chloroplasts from the viewpoint of lipid metabolism Reviewed

    BSJ-Review   12   23 - 37   2021.03

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  • Evolution and diversity of chloroplasts from the viewpoint of lipid metabolism

    小林康一, 吉原晶子

    植物科学の最前線(Web)   12   2021( ISSN:2432-9819

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  • Role of galactolipids in etioplast development and etioplast-to-chloroplast differentiation Invited Reviewed International journal

    28 ( 3 )   139 - 147   2018.12

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    Authorship:Last author, Corresponding author   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   International / domestic magazine:Domestic journal  

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Presentations

  • エチオプラストの発達や機能にホスファチジルグリセロールは必須なのか?

    川向真翔、吉原晶子、小林啓子、永田典子、小林康一

    第65回日本植物生理学会年会  2024.03 

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    Presentation type:Poster presentation  

  • シロイヌナズナの冠水時に起こるCO2欠乏に対する光合成の光保護機構

    戸次咲惠、大西亜依、和田元、小林康一

    第65回日本植物生理学会年会  2024.03 

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    Presentation type:Oral presentation (general)  

  • 葉緑体膜脂質であるDGDGとSQDGの機能的相補関係

    倉谷美帆、吉原晶子、小林啓子、永田典子、小林康一

    第65回日本植物生理学会年会  2024.03 

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    Presentation type:Oral presentation (general)  

  • Interplay of lipid classes in thylakoid membrane biogenesis and photosynthetic functioning Invited International conference

    Koichi Kobayashi

    IRN France-Japan Frontiers in Plant Biology (Kyoto)  2023.10 

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    Presentation type:Oral presentation (invited, special)  

  • Plastid phospholipid PG and sulfolipid SQDG are required for etioplast development and de-etiolation of Arabidopsis thaliana International conference

    Akiko Yoshihara, Keiko Kobayashi, Noriko Nagata, Sho Fujii, Hjime Wada, Koichi Kobayashi

    IRN France-Japan Frontiers in Plant Biology (Kyoto)  2023.10 

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    Presentation type:Poster presentation  

  • Protection of the photosynthetic machinery in response to CO2 limitation in submerged Arabidopsis thaliana seedlings International conference

    Sae Bekki, Ai Ohnishi, Hajime Wada and Koichi Kobayashi

    IRN France-Japan Frontiers in Plant Biology (Kyoto)  2023.10 

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    Presentation type:Poster presentation  

  • The sulfolipid SQDG compensates loss of DGDG in photosynthetic membranes International conference

    Miho Kuratani, Akiko Yoshihara and Koichi Kobayashi

    IRN France-Japan Frontiers in Plant Biology (Kyoto)  2023.10 

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    Presentation type:Poster presentation  

  • Galactolipid biosynthesis involves in GUN1-mediated regulation of photosynthesis-associated genes

    Sho Fujii, Hajime Wada and Koichi Kobayashi

    The 15th International Conference on Tetrapyrrole Photoreceptors in Photosynthetic Organism  2023.09 

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    Presentation type:Poster presentation  

  • Roles of plastid phospholipid PG and sulfolipid SQDG in etioplast development and de-etiolation of Arabidopsis thaliana

    Akiko Yoshihara, Keiko Kobayashi, Noriko Nagata, Sho Fujii, Hjime Wada, Koichi Kobayashi

    The 15th International Conference on Tetrapyrrole Photoreceptors in Photosynthetic Organism  2023.09 

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    Presentation type:Poster presentation  

  • 葉緑体形成における酸性リン脂質PGと酸性糖脂質SQDGの関係

    吉原晶子、小林啓子、永田典子、小林康一

    第87回日本植物学会年会  2023.09 

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    Presentation type:Oral presentation (general)  

  • 色素体の働き方改革~光合成は葉の専売特許ではない~

    小林康一

    第87回日本植物学会年会  2023.09 

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    Presentation type:Oral presentation (general)  

  • 色素体酸性脂質PG, SQDGはエチオプラスト-葉緑体分化時に重要な役割を担う

    吉原晶子、小林啓子、永田典子、小林康一

    第35回日本植物脂質シンポジウム  2023.09 

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    Presentation type:Oral presentation (general)  

  • チラコイド膜の形成過程においてホスファチジルグリセロールが果たす役割

    吉原晶子、藤井祥、上床理紗、小林啓子、永田典子、和田元、小林康一

    第35回日本植物脂質シンポジウム  2023.09 

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    Presentation type:Poster presentation  

  • 色素体における膜脂質合成と遺伝子発現の協調的制御

    藤井祥,野戸康生,黒滝悠志,和田元,小林康一

    第35回日本植物脂質シンポジウム  2023.09 

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    Presentation type:Poster presentation  

  • エチオプラスト-葉緑体分化における色素体酸性脂質PG, SQDGの役割

    吉原晶子、小林啓子、永田典子、小林康一

    第23回日本光生物学協会年会  2023.08 

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  • 葉緑体形成における色素体リン脂質PGの役割の解明

    吉原晶子、小林啓子、永田典子、小林康一

    第13回日本光合成学会年会  2023.06 

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  • 光合成複合体に含まれる脂質からひも解く、シアノバクテリアと植物との共通性と多様性 Invited Domestic conference

    小林康一

    2022年度ラン藻ゲノム交流会  2022.07 

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    Presentation type:Public lecture, seminar, tutorial, course, or other speech  

    Venue:東京/オンライン  

  • 光合成と細胞成長がシアノバクテリアSynechocystis sp. PCC 6803の窒素欠乏時のフィコビリソーム分解に与える影響 Domestic conference

    吉原晶子,小林康一

    第63回日本植物生理学会年会  2022.03 

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    Venue:つくば(オンライン)  

  • シロイヌナズナにおけるエチオプラスト発達時の色素体酸性脂質の役割 Domestic conference

    吉原晶子、永田典子、和田元、小林康一

    第63回日本植物生理学会年会  2022.03 

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    Presentation type:Oral presentation (general)  

    Venue:つくば(オンライン)  

  • 植物の発達における色素体酸性脂質の相補的関係 Domestic conference

    吉原晶子、永田典子、和田元、小林康一

    第33回植物脂質シンポジウム  2021.09 

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  • Are large budgets essential for modern biological studies? Invited Domestic conference

    22nd Young Researchers Seminar of Japanese Society of Photosynthesis Research  2021.05 

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    Presentation type:Oral presentation (invited, special)  

  • シアノバクテリアSynechocystis sp. PCC 6803において窒素欠乏下の光合成と細胞増殖はフィコビリソーム分解に関与する Domestic conference

    吉原晶子,小林康一

    第11回日本光合成学会年会・シンポジウム  2021.05  日本光合成学会

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  • 窒素欠乏時におけるシアノバクテリアSynechocystis sp. PCC 6803の細胞増殖とフィコビリソーム分解の関係 Domestic conference

    吉原晶子、小林康一

    第62回日本植物生理学会年会  2021.03 

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  • 色素体特有の酸性脂質は植物の非光合成器官の成長にも必須である Domestic conference

    吉原晶子、和田元、小林康一

    第62回日本植物生理学会年会  2021.03 

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  • STARTドメインをもつタンパク質がゼニゴケにおいて小胞体に由来する脂肪酸の葉緑体糖脂質への取り込みに関与している Domestic conference

    平嶋孝志、神保晴彦、小林康一、和田元

    第62回日本植物生理学会年会  2021.03 

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  • 地上部切除により誘導される根細胞での葉緑体の発達には小胞体からの脂質供給が欠かせない Domestic conference

    小畑智暉、小林康一、多田隈遼亮、赤坂泰輝、射場厚、祢宜淳太郎

    第62回日本植物生理学会年会  2021.03 

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  • ゼニゴケの葉緑体膜脂質代謝に関与するオルガネラ間脂質輸送タンパク質の解析 Domestic conference

    平嶋孝志、神保晴彦、小林康一、和田元

    日本植物学会第84回大会  2020.09 

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  • シロイヌナズナの芽生えの発達において色素体酸性脂質の果たす役割 Domestic conference

    吉原晶子、和田元、小林康一

    日本植物学会第84回大会  2020.09 

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  • 脂質代謝が印す葉緑体の進化と多様化 Domestic conference

    小林康一

    日本植物学会第84回大会  2020.09 

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    Presentation type:Oral presentation (invited, special)  

  • 葉緑体包膜脂質輸送体を欠損したシロイヌナズナ変異体gles1を用いた根細胞における葉緑体形成誘導の解析 Domestic conference

    小畑智暉、多田隈亮介、小林康一、射場厚、祢宜淳太郎

    第61回日本植物生理学会年会  2020.03 

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  • 黄化芽生えのエチオプラスト発達時における酸性膜脂質の役割 Domestic conference

    吉原晶子、和田元、小林康一

    第61回日本植物生理学会年会  2020.03 

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  • すべては脂質から始まる?葉緑体の初期発生 Domestic conference

    小林康一

    第61回日本植物生理学会年会  2020.03 

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    Presentation type:Oral presentation (invited, special)  

  • Roles of galactolipids in plastid development during skotomorphogenesis to photomorphogenesis International conference

    Akiko Yoshihara, Kaichiro Endo, Masato Abe, Hajime Wada, Koichi Kobayashi

    8th Asian Symposium on Plant Lipid  2019.11 

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  • Membrane lipids facilitate plastid differentiation before and after light exposure Invited International conference

    Koichi Kobayashi, Sho Fujii, Noriko Nagata, Tatsuru Masuda, Hajime Wada

    8th Asian Symposium on Plant Lipid  2019.11 

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  • 葉緑体分化過程におけるガラクト脂質合成と光合成関連遺伝子の発現との 関係 Domestic conference

    藤井祥、永田典子、増田建、和田元、小林康一

    第32回植物脂質シンポジウム  2019.09 

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  • STARTドメインをもつ脂質輸送タンパク質のゼニゴケにおける機能解析 Domestic conference

    平嶋孝志、神保晴彦、小林康一、和田元

    第32回植物脂質シンポジウム  2019.09 

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  • チラコイド膜構築過程における脂質合成 Domestic conference

    大沢祐香、小林康一、下嶋美恵、粟井光一郎

    第32回植物脂質シンポジウム  2019.09 

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  • ガラクト脂質が駆動するエチオプラストから葉緑体へのダイナミックな分化 Domestic conference

    藤井祥、永田典子、増田建、和田元、小林康一

    第31回植物脂質シンポジウム  2018 

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  • Distinct and overlapped roles of MGDG and DGDG in etioplasts of dark-grown Arabidopsis International conference

    Fujii Sho, Nagata Noriko, Masuda Tatsuru, Wada Hajime, Kobayashi Koichi

    23rd International Symposium on Plant Lipids  2018 

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  • Roles of galactolipids in plastid development during skotomorphogenesis to photomorphogenesis

    Fujii Sho, Nagata Noriko, Masuda Tatsuru, Wada Hajime, Kobayashi Koichi

    Japan-Finland Seminar 2018  2018 

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  • 子葉緑化時のクロロフィル合成と光合成遺伝子発現にガラクト脂質合成が与える影響 Domestic conference

    藤井祥、永田典子、増田建、和田元、小林康一

    日本植物学会第82回大会  2018 

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  • Regulatory mechanism of chloroplast development in Arabidopsis roots by a GATA transcription factor GNL Domestic conference

    大西亜依、小林康一、和田元

    第59回日本植物生理学会年会  2017 

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  • Distinct and overlapped roles of MGDG and DGDG in etioplasts of dark-grown Arabidopsis Invited International conference

    Sho Fujii, Noriko Nagata, Tatsuru Masuda, Hajime Wada and Koichi Kobayashi

    7th Asian Symposium on Plant Lipid  2017 

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  • 根が光合成を行うとき、その制御は葉とどのように違うのか? Domestic conference

    大西 亜依,和田 元,小林 康一

    日本植物学会第81回大会  2017 

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  • The leafless mycoheterotrophic orchid Cymbidium macrorhizon performs photosynthesis during fruiting season. Domestic conference

    Koichi Kobayashi, Kenji Suetsugu, Hajime Wada

    第58回植物生理学会年会  2016 

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  • Specific roles and functional overlaps of thylakoid anionic lipids International conference

    Koichi Kobayashi, Kaichiro Endo, Sho Fujii, and Hajime Wada

    22nd International Symposium on Plant Lipids  2016 

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  • Regulation of chloroplast differentiation in Arabidopsis roots in response to shoot removal International conference

    Koichi Kobayashi, Ai Ohnishi, Daichi Sasaki, Sho Fujii, Akira Iwase, Keiko Sugimoto, Tatsuru Masuda, and Hajime Wada

    CSHA Symposium, Latest Advances in Plant Development & Environmental Response  2016 

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  • 植物細胞における PGの合成経路とその機能 Domestic conference

    小林康一、遠藤嘉一郎、藤井祥、Ying-Chen Lin、Chun-Hsien Hung、 中村友輝、和田元

    第29回植物脂質シンポジウム  2016 

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  • Specific roles and functional overlaps of thylakoid anionic lipids in Arabidopsis Domestic conference

    Koichi Kobayashi, Kaichiro Endo, Hajime Wada

    第57回日本植物生理学会年会  2015 

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  • シロイヌナズナの根でみる光と植物ホルモンによる光合成の制御 Domestic conference

    小林康一、大西亜依、藤井祥、和田元

    日本植物学会第79回大会  2015 

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  • 光合成生物におけるPGとSQDGの機能 Domestic conference

    小林康一、遠藤嘉一郎、和田元

    第28回植物脂質シンポジウム  2015 

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  • Regulation of chloroplast differentiation in Arabidopsis roots Invited International conference

    K Kobayashi, T Masuda, H Wada

    Japanese-Finnish Seminar 2014  2014 

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  • シロイヌナズナの葉緑体バイオジェネシスにおける酸性膜脂質の役割 Domestic conference

    小林康一、藤井祥、堀遥香、和田元

    日本植物学会第78回大会  2014 

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  • シロイヌナズナを用いた葉緑体発達制御機構の解明 Invited Domestic conference

    小林康一

    日本植物学会第78回大会  2014 

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  • 葉緑体機能におけるPGと糖脂質の関係 Domestic conference

    小林康一、藤井祥、遠藤嘉一郎、堀遥香、和田元

    第27回植物脂質シンポジウム  2014 

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  • Coordination of galactolipid synthesis with formation of photosynthetic complexes and organelle differentiation International conference

    K Kobayashi, S Fujii, Y Nakamura, T Masuda, H Ohta, H Wada

    21st International Symposium on Plant Lipids  2014 

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Outline of collaborative research (seeds)

  • 葉緑体の分化制御機構の解明

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    植物がどのような環境やシグナルによって葉緑体機能を発現し光合成をするのかを研究している。

Charge of on-campus class subject

  • Fundamental Experiments of Biology

    2021   Practical Training  

  • Fundamental Experiments of Biology

    2021   Practical Training  

  • Biology B

    2021    

  • Biology A

    2021    

  • Plant physiology

    2021    

  • Biology A

    2021    

  • Fundamental Experiments of Biology

    2021   Practical Training  

  • Fundamental Experiments of Biology

    2021   Practical Training  

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