2023/01/30 更新

写真a

ツシマ アヤコ
津島 綾子
TSUSHIMA AYAKO
担当
大学院農学研究科 応用生物科学専攻 助教
農学部 応用生物科学科
職名
助教
所属
農学研究院

担当・職階

  • 大学院農学研究科 応用生物科学専攻 

    助教  2022年04月 - 継続中

  • 農学部 応用生物科学科 

    助教  2022年04月 - 継続中

取得学位

  • 博士(理学) ( 東京大学 )

受賞歴

  • 平成28年度日本植物病理学会大会 学生優秀発表賞

    2016年05月  

  • 平成27年度植物感染生理談話会 優秀発表賞

    2015年08月  

職務経歴(学外)

  • 大阪公立大学

    2022年04月 - 継続中

  • ジョンイネスセンター   博士研究員

    2020年01月 - 2022年03月

  • 理化学研究所   特別研究員

    2019年07月 - 2019年12月

学歴

  • 東京大学   理学系研究科   生物科学専攻   博士課程   卒業・修了

    2016年04月 - 2019年05月

論文

  • Genomic resources of Colletotrichum fungi: development and application

    Ayako Tsushima, Ken Shirasu

    Journal of General Plant Pathology   2022年10月( ISSN:1345-2630

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1007/s10327-022-0

  • Wheat stem rust recorded for the first time in decades in Ireland

    Ayako Tsushima, Clare M. Lewis, Kerstin Flath, Stephen Kildea, Diane G.O. Saunders

    Plant Pathology   2022年01月( ISSN:0032-0862

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/ppa.13532

  • The Conserved Colletotrichum spp. Effector Candidate CEC3 Induces Nuclear Expansion and Cell Death in Plants

    Ayako Tsushima, Mari Narusaka, Pamela Gan, Naoyoshi Kumakura, Ryoko Hiroyama, Naoki Kato, Shunji Takahashi, Yoshitaka Takano, Yoshihiro Narusaka, Ken Shirasu

    Frontiers in Microbiology   12   682155 - 682155   2021年09月( ISSN:1664-302X

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    <jats:p>Plant pathogens secrete proteins, known as effectors, that promote infection by manipulating host cells. Members of the phytopathogenic fungal genus <jats:italic>Colletotrichum</jats:italic> collectively have a broad host range and generally adopt a hemibiotrophic lifestyle that includes an initial biotrophic phase and a later necrotrophic phase. We hypothesized that <jats:italic>Colletotrichum</jats:italic> fungi use a set of conserved effectors during infection to support the two phases of their hemibiotrophic lifestyle. This study aimed to examine this hypothesis by identifying and characterizing conserved effectors among <jats:italic>Colletotrichum</jats:italic> fungi. Comparative genomic analyses using genomes of ascomycete fungi with different lifestyles identified seven effector candidates that are conserved across the genus <jats:italic>Colletotrichum</jats:italic>. Transient expression assays showed that one of these putative conserved effectors, CEC3, induces nuclear expansion and cell death in <jats:italic>Nicotiana benthamiana</jats:italic>, suggesting that CEC3 is involved in promoting host cell death during infection. Nuclear expansion and cell death induction were commonly observed in CEC3 homologs from four different <jats:italic>Colletotrichum</jats:italic> species that vary in host specificity. Thus, CEC3 proteins could represent a novel class of core effectors with functional conservation in the genus C<jats:italic>olletotrichum</jats:italic>.</jats:p>

    DOI: 10.3389/fmicb.2021.682155

    PubMed

  • A pair of effectors encoded on a conditionally dispensable chromosome of Fusarium oxysporum suppress host-specific immunity

    Yu Ayukawa, Shuta Asai, Pamela Gan, Ayako Tsushima, Yasunori Ichihashi, Arisa Shibata, Ken Komatsu, Petra M. Houterman, Martijn Rep, Ken Shirasu, Tsutomu Arie

    Communications Biology   4 ( 1 )   707 - 707   2021年06月( ISSN:2399-3642

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    <jats:title>Abstract</jats:title><jats:p>Many plant pathogenic fungi contain conditionally dispensable (CD) chromosomes that are associated with virulence, but not growth in vitro. Virulence-associated CD chromosomes carry genes encoding effectors and/or host-specific toxin biosynthesis enzymes that may contribute to determining host specificity. <jats:italic>Fusarium oxysporum</jats:italic> causes devastating diseases of more than 100 plant species. Among a large number of host-specific forms, <jats:italic>F. oxysporum</jats:italic> f. sp. <jats:italic>conglutinans</jats:italic> (<jats:italic>Focn</jats:italic>) can infect Brassicaceae plants including Arabidopsis (<jats:italic>Arabidopsis thaliana</jats:italic>) and cabbage. Here we show that <jats:italic>Focn</jats:italic> has multiple CD chromosomes. We identified specific CD chromosomes that are required for virulence on Arabidopsis, cabbage, or both, and describe a pair of effectors encoded on one of the CD chromosomes that is required for suppression of Arabidopsis-specific phytoalexin-based immunity. The effector pair is highly conserved in <jats:italic>F. oxysporum</jats:italic> isolates capable of infecting Arabidopsis, but not of other plants. This study provides insight into how host specificity of <jats:italic>F. oxysporum</jats:italic> may be determined by a pair of effector genes on a transmissible CD chromosome.</jats:p>

    DOI: 10.1038/s42003-021-02245-4

    PubMed

  • Telomeres and a repeat‐rich chromosome encode effector gene clusters in plant pathogenicColletotrichumfungi

    Pamela Gan, Ryoko Hiroyama, Ayako Tsushima, Sachiko Masuda, Arisa Shibata, Akiko Ueno, Naoyoshi Kumakura, Mari Narusaka, Trinh Xuan Hoat, Yoshihiro Narusaka, Yoshitaka Takano, Ken Shirasu

    Environmental Microbiology   2021年04月( ISSN:1462-2912

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/1462-2920.15490

    その他URL: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.15490

  • Colletotrichum shisoi sp. nov., an anthracnose pathogen of Perilla frutescens in Japan: molecular phylogenetic, morphological and genomic evidence

    P. Gan, A. Tsushima, R. Hiroyama, M. Narusaka, Y. Takano, Y. Narusaka, M. Kawaradani, U. Damm, K. Shirasu

    Scientific Reports   9 ( 1 )   2019年12月( ISSN:2045-2322

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    掲載種別:研究論文(学術雑誌)  

    Abstract

    Species of the fungal genus Colletotrichum are among the most devastating pathogens of agricultural crops in the world. Based on DNA sequence data (ITS, GAPDH, CHS-1, ACT, TUB2) and morphology, we revealed Colletotrichum isolates infecting the oil crop Perilla frutescens, commonly known as shiso, to represent a previously unknown species of the C. destructivum species complex and described it as C. shisoi. We found that C. shisoi appears to be able to adopt a hemibiotrophic lifestyle, characterised by the formation of biotrophic hyphae followed by severe necrotic lesions on P. frutescens, but is less virulent on Arabidopsis, compared to its close relative C. higginsianum which also belongs to the C. destructivum species complex. The genome of C. shisoi was sequenced, annotated and its predicted proteome compared with four other Colletotrichum species. The predicted proteomes of C. shisoi and C. higginsianum, share many candidate effectors, which are small, secreted proteins that may contribute to infection. Interestingly, C. destructivum species complex-specific secreted proteins showed evidence of increased diversifying selection which may be related to their host specificities.

    DOI: 10.1038/s41598-019-50076-5

    その他URL: http://www.nature.com/articles/s41598-019-50076-5

  • Genome Sequence Resources for Four Phytopathogenic Fungi from the <i>Colletotrichum orbiculare</i> Species Complex

    P. Gan, A. Tsushima, M. Narusaka, Y. Narusaka, Y. Takano, Y. Kubo, K. Shirasu

    Molecular Plant-Microbe Interactions®   32 ( 9 )   1088 - 1090   2019年09月( ISSN:0894-0282

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    掲載種別:研究論文(学術雑誌)  

    Colletotrichum orbiculare species complex fungi are hemibiotrophic plant pathogens that cause anthracnose of field crops and weeds. Members of this group have genomes that are remarkably expanded relative to other Colletotrichum fungi and compartmentalized into AT-rich, gene-poor and GC-rich, gene-rich regions. Here, we present an updated version of the C. orbiculare genome, as well as draft genomes of three other members from the C. orbiculare species complex: the alfalfa pathogen C. trifolii, the prickly mallow pathogen C. sidae, and the burweed pathogen C. spinosum. The data reported here will be important for comparative genomics analyses to identify factors that play a role in the evolution and maintenance of the expanded, compartmentalized genomes of these fungi, which may contribute to their pathogenicity.

    DOI: 10.1094/mpmi-12-18-0352-a

  • Genomic Plasticity Mediated by Transposable Elements in the Plant Pathogenic Fungus Colletotrichum higginsianum

    Ayako Tsushima, Pamela Gan, Naoyoshi Kumakura, Mari Narusaka, Yoshitaka Takano, Yoshihiro Narusaka, Ken Shirasu

    Genome Biology and Evolution   11 ( 5 )   1487 - 1500   2019年05月( ISSN:1759-6653

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/gbe/evz087

    その他URL: http://academic.oup.com/gbe/article-pdf/11/5/1487/28705793/evz087.pdf

  • Method for Assessing Virulence of Colletotrichum higginsianum on Arabidopsis thaliana Leaves Using Automated Lesion Area Detection and Measurement

    Ayako Tsushima, Pamela Gan, Ken Shirasu

    BIO-PROTOCOL   9 ( 22 )   2019年( ISSN:2331-8325

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.21769/bioprotoc.3434

  • Draft Genome Assembly of <i>Colletotrichum chlorophyti</i> , a Pathogen of Herbaceous Plants

    P. Gan, M. Narusaka, A. Tsushima, Y. Narusaka, Y. Takano, K. Shirasu

    Genome Announcements   5 ( 10 )   2017年03月

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    掲載種別:研究論文(学術雑誌)  

    ABSTRACT

    Colletotrichum chlorophyti is a fungal pathogen that infects various herbaceous plants, including crops such as legumes, tomato, and soybean. Here, we present the genome of C. chlorophyti NTL11, isolated from tomato. Analysis of this genome will allow a clearer understanding of the molecular mechanisms underlying fungal host range and pathogenicity.

    DOI: 10.1128/genomea.01733-16

  • Genus-Wide Comparative Genome Analyses of<i>Colletotrichum</i>Species Reveal Specific Gene Family Losses and Gains during Adaptation to Specific Infection Lifestyles

    Pamela Gan, Mari Narusaka, Naoyoshi Kumakura, Ayako Tsushima, Yoshitaka Takano, Yoshihiro Narusaka, Ken Shirasu

    Genome Biology and Evolution   8 ( 5 )   1467 - 1481   2016年05月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/gbe/evw089

▼全件表示

講演・口頭発表等

  • アイルランドで発生したコムギ黒さび病菌のトランスクリプトームデータを用いた系統解析

    津島綾子, Clare M. Lewis, Kerstin Flath, Stephen Kildea, Diane G.O. Saunders

    令和4年度日本植物病理学会大会  2022年03月 

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    会議種別:口頭発表(一般)  

  • 植物病原真菌のゲノム情報の取得と活用 招待

    津島綾子

    第5回 植物病理を紡ぐ会  2022年03月 

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    会議種別:公開講演,セミナー,チュートリアル,講習,講義等  

  • 野外トランスクリプトームデータを利用したコムギ黒さび病の診断と発生調査 招待

    津島綾子, Clare M. Lewis, Kerstin Flath, Stephen Kildea, Diane G.O. Saunders

    第63回日本植物生理学会年会  2022年03月 

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    会議種別:口頭発表(招待・特別)