2024/03/25 更新

写真a

キシモト マイ
岸本 麻衣
Mai Kishimoto
担当
大学院獣医学研究科 獣医学専攻 助教
獣医学部 獣医学科
職名
助教
所属
獣医学研究院
所属キャンパス
りんくうキャンパス

担当・職階

  • 大学院獣医学研究科 獣医学専攻 

    助教  2023年04月 - 継続中

  • 獣医学部 獣医学科 

    助教  2023年04月 - 継続中

取得学位

  • 博士(獣医学) ( 北海道大学 )

  • 学士(獣医学) ( 東京農工大学 )

研究分野

  • ライフサイエンス / 獣医学  / 微生物学

  • ライフサイエンス / ウイルス学  / 人獣共通感染症

受賞歴

  • 第166回日本獣医学会学術集会優秀発表賞

    2023年11月   日本獣医学会   ザンビアのエジプトルーセットオオコウモリおよびマストミスから分離されたロタウイルス A の性状解析

  • 大塚賞

    2023年03月   北海道大学  

  • 第7回微生物学分科会若手奨励賞

    2016年09月   日本獣医学会  

職務経歴(学外)

  • 日本学術振興会特別研究員(DC1)

    2021年04月 - 2023年03月

  • 警察庁 科学警察研究所

    2017年04月 - 2019年09月

論文

  • Isolation and Characterization of Distinct Rotavirus A in Bat and Rodent Hosts. 査読 国際共著

    Mai Kishimoto, Masahiro Kajihara, Koshiro Tabata, Yukari Itakura, Shinsuke Toba, Seiya Ozono, Yuko Sato, Tadaki Suzuki, Naoto Ito, Katendi Changula, Yongjin Qiu, Akina Mori-Kajihara, Yoshiki Eto, Hayato Harima, Daniel Mwizabi, Bernard M Hang'ombe, William W Hall, Ayato Takada, Yasuko Orba, Hirofumi Sawa, Michihito Sasaki

    Journal of virology   97 ( 1 )   e0145522   2023年01月

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    担当区分:筆頭著者   掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    Rotavirus A (RVA) causes diarrheal disease in humans and various animals. Recent studies have identified bat and rodent RVAs with evidence of zoonotic transmission and genome reassortment. However, the virological properties of bat and rodent RVAs with currently identified genotypes still need to be better clarified. Here, we performed virus isolation-based screening for RVA in animal specimens and isolated RVAs (representative strains: 16-06 and MpR12) from Egyptian fruit bat and Natal multimammate mouse collected in Zambia. Whole-genome sequencing and phylogenetic analysis revealed that the genotypes of bat RVA 16-06 were identical to that of RVA BATp39 strain from the Kenyan fruit bat, which has not yet been characterized. Moreover, all segments of rodent RVA MpR12 were highly divergent and assigned to novel genotypes, but RVA MpR12 was phylogenetically closer to bat RVAs than to other rodent RVAs, indicating a unique evolutionary history. We further investigated the virological properties of the isolated RVAs. In brief, we found that 16-06 entered cells by binding to sialic acids on the cell surface, while MpR12 entered in a sialic acid-independent manner. Experimental inoculation of suckling mice with 16-06 and MpR12 revealed that these RVAs are causative agents of diarrhea. Moreover, 16-06 and MpR12 demonstrated an ability to infect and replicate in a 3D-reconstructed primary human intestinal epithelium with comparable efficiency to the human RVA. Taken together, our results detail the unique genetic and virological features of bat and rodent RVAs and demonstrate the need for further investigation of their zoonotic potential. IMPORTANCE Recent advances in nucleotide sequence detection methods have enabled the detection of RVA genomes from various animals. These studies have discovered multiple divergent RVAs and have resulted in proposals for the genetic classification of novel genotypes. However, most of these RVAs have been identified via dsRNA viral genomes and not from infectious viruses, and their virological properties, such as cell/host tropisms, transmissibility, and pathogenicity, are unclear and remain to be clarified. Here, we successfully isolated RVAs with novel genome constellations from three bats and one rodent in Zambia. In addition to whole-genome sequencing, the isolated RVAs were characterized by glycan-binding affinity, pathogenicity in mice, and infectivity to the human gut using a 3D culture of primary intestinal epithelium. Our study reveals the first virological properties of bat and rodent RVAs with high genetic diversity and unique evolutional history and provides basic knowledge to begin estimating the potential of zoonotic transmission.

    DOI: 10.1128/jvi.01455-22

    PubMed

  • Mastomys natalensis is a possible natural rodent reservoir for encephalomyocarditis virus. 査読 国際共著

    Mai Kishimoto, Bernard M Hang'ombe, William W Hall, Yasuko Orba, Hirofumi Sawa, Michihito Sasaki

    The Journal of general virology   102 ( 3 )   2021年03月

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    担当区分:筆頭著者   掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    Encephalomyocarditis virus (EMCV) infects a wide range of hosts and can cause encephalitis, myocarditis, reproductive disorders and diabetes mellitus in selected mammalian species. As for humans, EMCV infection seems to occur by the contact with animals and can cause febrile illnesses in some infected patients. Here we isolated EMCV strain ZM12/14 from a natal multimammate mouse (Mastomys natalensis: M. natalensis) in Zambia. Pairwise sequence similarity of the ZM12/14 P1 region consisting of antigenic capsid proteins showed the highest similarity of nucleotide (80.7 %) and amino acid (96.2%) sequence with EMCV serotype 1 (EMCV-1). Phylogenetic analysis revealed that ZM12/14 clustered into EMCV-1 at the P1 and P3 regions but segregated from known EMCV strains at the P2 region, suggesting a unique evolutionary history. Reverse transcription PCR (RT-PCR) screening and neutralizing antibody assays for EMCV were performed using collected tissues and serum from various rodents (n=179) captured in different areas in Zambia. We detected the EMCV genome in 19 M. natalensis (19/179=10.6 %) and neutralizing antibody for EMCV in 33 M. natalensis (33/179=18.4 %). However, we did not detect either the genome or neutralizing antibody in other rodent species. High neutralizing antibody litres (≧320) were observed in both RT-PCR-negative and -positive animals. Inoculation of ZM12/14 caused asymptomatic persistent infection in BALB/c mice with high antibody titres and high viral loads in some organs, consistent with the above epidemiological results. This study is the first report of the isolation of EMCV in Zambia, suggesting that M. natalensis may play a role as a natural reservoir of infection.

    DOI: 10.1099/jgv.0.001564

    PubMed

  • TMPRSS11D and TMPRSS13 Activate the SARS-CoV-2 Spike Protein. 査読 国際共著

    Mai Kishimoto, Kentaro Uemura, Takao Sanaki, Akihiko Sato, William W Hall, Hiroaki Kariwa, Yasuko Orba, Hirofumi Sawa, Michihito Sasaki

    Viruses   13 ( 3 )   2021年02月

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    担当区分:筆頭著者   掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) utilizes host proteases, including a plasma membrane-associated transmembrane protease, serine 2 (TMPRSS2) to cleave and activate the virus spike protein to facilitate cellular entry. Although TMPRSS2 is a well-characterized type II transmembrane serine protease (TTSP), the role of other TTSPs on the replication of SARS-CoV-2 remains to be elucidated. Here, we have screened 12 TTSPs using human angiotensin-converting enzyme 2-expressing HEK293T (293T-ACE2) cells and Vero E6 cells and demonstrated that exogenous expression of TMPRSS11D and TMPRSS13 enhanced cellular uptake and subsequent replication of SARS-CoV-2. In addition, SARS-CoV-1 and SARS-CoV-2 share the same TTSPs in the viral entry process. Our study demonstrates the impact of host TTSPs on infection of SARS-CoV-2, which may have implications for cell and tissue tropism, for pathogenicity, and potentially for vaccine development.

    DOI: 10.3390/v13030384

    PubMed

  • Development of a one-run real-time PCR detection system for pathogens associated with bovine respiratory disease complex. 査読

    Mai Kishimoto, Shinobu Tsuchiaka, Sayed Samim Rahpaya, Ayako Hasebe, Keiko Otsu, Satoshi Sugimura, Suguru Kobayashi, Natsumi Komatsu, Makoto Nagai, Tsutomu Omatsu, Yuki Naoi, Kaori Sano, Sachiko Okazaki-Terashima, Mami Oba, Yukie Katayama, Reiichiro Sato, Tetsuo Asai, Tetsuya Mizutani

    The Journal of veterinary medical science   79 ( 3 )   517 - 523   2017年03月

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    担当区分:筆頭著者   掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    DOI: 10.1292/jvms.16-0489

  • Combination therapy with oral antiviral and anti-inflammatory drugs improves the efficacy of delayed treatment in a COVID-19 hamster model. 査読 国際共著

    Michihito Sasaki, Tatsuki Sugi, Shun Iida, Yuichiro Hirata, Shinji Kusakabe, Kei Konishi, Yukari Itakura, Koshiro Tabata, Mai Kishimoto, Hiroko Kobayashi, Takuma Ariizumi, Kittiya Intaruck, Haruaki Nobori, Shinsuke Toba, Akihiko Sato, Keita Matsuno, Junya Yamagishi, Tadaki Suzuki, William W Hall, Yasuko Orba, Hirofumi Sawa

    EBioMedicine   99   104950 - 104950   2023年12月

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    BACKGROUND: Pulmonary infection with SARS-CoV-2 stimulates host immune responses and can also result in the progression of dysregulated and critical inflammation. Throughout the pandemic, the management and treatment of COVID-19 has been continuously updated with a range of antiviral drugs and immunomodulators. Monotherapy with oral antivirals has proven to be effective in the treatment of COVID-19. However, treatment should be initiated in the early stages of infection to ensure beneficial therapeutic outcomes, and there is still room for further consideration on therapeutic strategies using antivirals. METHODS: We studied the therapeutic effects of monotherapy with the oral antiviral ensitrelvir or the anti-inflammatory corticosteroid methylprednisolone and combination therapy with ensitrelvir and methylprednisolone in a delayed dosing model of hamsters infected with SARS-CoV-2. FINDINGS: Combination therapy with ensitrelvir and methylprednisolone improved respiratory conditions and reduced the development of pneumonia in hamsters even when the treatment was started after 2 days post-infection. The combination therapy led to a differential histological and transcriptomic pattern in comparison to either of the monotherapies, with reduced lung damage and down-regulation of expression of genes involved in the inflammatory response. Furthermore, we found that the combination treatment is effective in case of infection with either the highly pathogenic delta or circulating omicron variants. INTERPRETATION: Our results demonstrate the advantage of combination therapy with antiviral and corticosteroid drugs in COVID-19 treatment from the perspective of lung pathology and host inflammatory responses. FUNDING: Funding bodies are described in the Acknowledgments section.

    DOI: 10.1016/j.ebiom.2023.104950

    PubMed

  • Development of flavivirus subviral particles with low cross-reactivity by mutations of a distinct antigenic domain. 査読 国際共著

    Koshiro Tabata, Yukari Itakura, Takuma Ariizumi, Manabu Igarashi, Hiroko Kobayashi, Kittiya Intaruck, Mai Kishimoto, Shintaro Kobayashi, William W Hall, Michihito Sasaki, Hirofumi Sawa, Yasuko Orba

    Applied microbiology and biotechnology   107 ( 24 )   7515 - 7529   2023年10月( ISSN:01757598

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    The most conserved fusion loop (FL) domain present in the flavivirus envelope protein has been reported as a dominant epitope for cross-reactive antibodies to mosquito-borne flaviviruses (MBFVs). As a result, establishing accurate serodiagnosis for MBFV infections has been difficult as anti-FL antibodies are induced by both natural infection and following vaccination. In this study, we modified the most conserved FL domain to overcome this cross-reactivity. We showed that the FL domain of lineage I insect-specific flavivirus (ISFV) has differences in antigenicity from those of MBFVs and lineage II ISFV and determined the key amino acid residues (G106, L107, or F108), which contribute to the antigenic difference. These mutations were subsequently introduced into subviral particles (SVPs) of dengue virus type 2 (DENV2), Zika virus (ZIKV), Japanese encephalitis virus (JEV), and West Nile virus (WNV). In indirect enzyme-linked immunosorbent assays (ELISAs), these SVP mutants when used as antigens reduced the binding of cross-reactive IgG and total Ig induced by infection of ZIKV, JEV, and WNV in mice and enabled the sensitive detection of virus-specific antibodies. Furthermore, immunization of ZIKV or JEV SVP mutants provoked the production of antibodies with lower cross-reactivity to heterologous MBFV antigens compared to immunization with the wild-type SVPs in mice. This study highlights the effectiveness of introducing mutations in the FL domain in MBFV SVPs with lineage I ISFV-derived amino acids to produce SVP antigens with low cross-reactivity and demonstrates an improvement in the accuracy of indirect ELISA-based serodiagnosis for MBFV infections. KEY POINTS: • The FL domain of Lineage I ISFV has a different antigenicity from that of MBFVs. • Mutated SVPs reduce the binding of cross-reactive antibodies in indirect ELISAs. • Inoculation of mutated SVPs induces antibodies with low cross-reactivity.

    DOI: 10.1007/s00253-023-12817-5

    PubMed

  • Comparative pathogenicity of SARS-CoV-2 Omicron subvariants including BA.1, BA.2, and BA.5 査読

    Tomokazu Tamura, Daichi Yamasoba, Yoshitaka Oda, Jumpei Ito, Tomoko Kamasaki, Naganori Nao, Rina Hashimoto, Yoichiro Fujioka, Rigel Suzuki, Lei Wang, Hayato Ito, Yukie Kashima, Izumi Kimura, Mai Kishimoto, Masumi Tsuda, Hirofumi Sawa, Kumiko Yoshimatsu, Yuki Yamamoto, Tetsuharu Nagamoto, Jun Kanamune, Yutaka Suzuki, Yusuke Ohba, Saori Suzuki, Marie Kato, Zannatul Ferdous, Hiromi Mouri, Kenji Shishido, Naoko Misawa, Keiya Uriu, Yusuke Kosugi, Shigeru Fujita, Mai Suganami, Mika Chiba, Ryo Yoshimura, So Nakagawa, Jiaqi Wu, Akifumi Takaori-Kondo, Kotaro Shirakawa, Kayoko Nagata, Yasuhiro Kazuma, Ryosuke Nomura, Yoshihito Horisawa, Yusuke Tashiro, Yugo Kawai, Takao Hashiguchi, Tateki Suzuki, Kanako Kimura, Jiei Sasaki, Yukari Nakajima, Ayaka Sakamoto, Naoko Yasuhara, Takashi Irie, Ryoko Kawabata, Terumasa Ikeda, Hesham Nasser, Ryo Shimizu, Monira Begum, Otowa Takahashi, Kimiko Ichihara, Takamasa Ueno, Chihiro Motozono, Mako Toyoda, Akatsuki Saito, Yuri L. Tanaka, Erika P. Butlertanaka, Maya Shofa, Kaori Tabata, Isao Yokota, Keita Matsuno, Kazuo Takayama, Shinya Tanaka, Kei Sato, Takasuke Fukuhara

    Communications Biology   6 ( 1 )   2023年07月

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    Abstract

    The unremitting emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants necessitates ongoing control measures. Given its rapid spread, the new Omicron subvariant BA.5 requires urgent characterization. Here, we comprehensively analyzed BA.5 with the other Omicron variants BA.1, BA.2, and ancestral B.1.1. Although in vitro growth kinetics of BA.5 was comparable among the Omicron subvariants, BA.5 was much more fusogenic than BA.1 and BA.2. Airway-on-a-chip analysis showed that, among Omicron subvariants, BA.5 had enhanced ability to disrupt the respiratory epithelial and endothelial barriers. Furthermore, in our hamster model, in vivo pathogenicity of BA.5 was slightly higher than that of the other Omicron variants and less than that of ancestral B.1.1. Notably, BA.5 gains efficient virus spread compared with BA.1 and BA.2, leading to prompt immune responses. Our findings suggest that BA.5 has low pathogenicity compared with the ancestral strain but enhanced virus spread /inflammation compared with earlier Omicron subvariants.

    DOI: 10.1038/s42003-023-05081-w

    その他URL: https://www.nature.com/articles/s42003-023-05081-w

  • Impact of imprinted immunity induced by mRNA vaccination in an experimental animal model. 査読

    Shigeru Fujita, Keiya Uriu, Lin Pan, Naganori Nao, Koshiro Tabata, Mai Kishimoto, Yukari Itakura, Hirofumi Sawa, Izumi Kida, Tomokazu Tamura, Takasuke Fukuhara, Jumpei Ito, Keita Matsuno, Kei Sato

    The Journal of infectious diseases   2023年06月

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    The emergence of SARS-CoV-2 Omicron variants has led to concerns that ancestral SARS-CoV-2-based vaccines may not be effective against newly emerging Omicron subvariants. The concept of "imprinted immunity" suggests that individuals vaccinated with ancestral virus-based vaccines may not develop effective immunity against newly emerging Omicron subvariants, such as BQ.1.1 and XBB.1. Here, we investigated this possibility using hamsters. While natural infection induced effective antiviral immunity, breakthrough infections in hamsters with BQ.1.1 and XBB.1 Omicron subvariants after receiving the 3-dose mRNA-LNP vaccine resulted in only faintly induced humoral immunity, supporting the possibility of imprinted immunity.

    DOI: 10.1093/infdis/jiad230

    PubMed

  • Morphogenesis of Bullet-Shaped Rabies Virus Particles Regulated by TSG101. 査読 国際共著

    Yukari Itakura, Koshiro Tabata, Takeshi Saito, Kittiya Intaruck, Nijiho Kawaguchi, Mai Kishimoto, Shiho Torii, Shintaro Kobayashi, Naoto Ito, Michiko Harada, Satoshi Inoue, Ken Maeda, Ayato Takada, William W Hall, Yasuko Orba, Hirofumi Sawa, Michihito Sasaki

    Journal of virology   97 ( 5 )   e0043823   2023年04月

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    Viral protein assembly and virion budding are tightly regulated to enable the proper formation of progeny virions. At this late stage in the virus life cycle, some enveloped viruses take advantage of the host endosomal sorting complex required for transport (ESCRT) machinery, which contributes to the physiological functions of membrane modulation and abscission. Bullet-shaped viral particles are unique morphological characteristics of rhabdoviruses; however, the involvement of host factors in rhabdovirus infection and, specifically, the molecular mechanisms underlying virion formation are not fully understood. In the present study, we used a small interfering RNA (siRNA) screening approach and found that the ESCRT-I component TSG101 contributes to the propagation of rabies virus (RABV). We demonstrated that the matrix protein (M) of RABV interacts with TSG101 via the late domain containing the PY and YL motifs, which are conserved in various viral proteins. Loss of the YL motif in the RABV M or the downregulation of host TSG101 expression resulted in the intracellular aggregation of viral proteins and abnormal virus particle formation, indicating a defect in the RABV assembly and budding processes. These results indicate that the interaction of the RABV M and TSG101 is pivotal for not only the efficient budding of progeny RABV from infected cells but also for the bullet-shaped virion morphology. IMPORTANCE Enveloped viruses bud from cells with the host lipid bilayer. Generally, the membrane modulation and abscission are mediated by host ESCRT complexes. Some enveloped viruses utilize their late (L-) domain to interact with ESCRTs, which promotes viral budding. Rhabdoviruses form characteristic bullet-shaped enveloped virions, but the underlying molecular mechanisms involved remain elusive. Here, we showed that TSG101, one of the ESCRT components, supports rabies virus (RABV) budding and proliferation. TSG101 interacted with RABV matrix protein via the L-domain, and the absence of this interaction resulted in intracellular virion accumulation and distortion of the morphology of progeny virions. Our study reveals that virion formation of RABV is highly regulated by TSG101 and the virus matrix protein.

    DOI: 10.1128/jvi.00438-23

    PubMed

  • Monitoring fusion kinetics of viral and target cell membranes in living cells using a SARS-CoV-2 spike-protein-mediated membrane fusion assay 査読

    Hesham Nasser, Ryo Shimizu, Jumpei Ito, Akatsuki Saito, Kei Sato, Terumasa Ikeda, Keita Matsuno, Naganori Nao, Hirofumi Sawa, Mai Kishimoto, Shinya Tanaka, Masumi Tsuda, Lei Wang, Yoshikata Oda, Marie Kato, Zannatul Ferdous, Hiromi Mouri, Kenji Shishido, Takasuke Fukuhara, Tomokazu Tamura, Rigel Suzuki, Hayato Ito, Daichi Yamasoba, Izumi Kimura, Naoko Misawa, Keiya Uriu, Yusuke Kosugi, Shigeru Fujita, Mai Suganami, Mika Chiba, Ryo Yoshimura, So Nakagawa, Jiaqi Wu, Akifumi Takaori-Kondo, Kotaro Shirakawa, Kayoko Nagata, Yasuhiro Kazuma, Ryosuke Nomura, Yoshihito Horisawa, Yusuke Tashiro, Yugo Kawai, Takashi Irie, Ryoko Kawabata, MST Monira Begum, Otowa Takahashi, Kimiko Ichihara, Takamasa Ueno, Chihiro Motozono, Mako Toyoda, Yuri L. Tanaka, Erika P. Butlertanaka, Maya Shofa, Kazuo Takayama, Rina Hashimoto, Sayaka Deguchi, Takao Hashiguchi, Tateki Suzuki, Kanako Kimura, Jiei Sasaki, Yukari Nakajima, Kaori Tabata

    STAR Protocols   3 ( 4 )   101773 - 101773   2022年12月( ISSN:2666-1667

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    DOI: 10.1016/j.xpro.2022.101773

  • S-217622, a SARS-CoV-2 main protease inhibitor, decreases viral load and ameliorates COVID-19 severity in hamsters. 査読 国際共著

    Michihito Sasaki, Koshiro Tabata, Mai Kishimoto, Yukari Itakura, Hiroko Kobayashi, Takuma Ariizumi, Kentaro Uemura, Shinsuke Toba, Shinji Kusakabe, Yuki Maruyama, Shun Iida, Noriko Nakajima, Tadaki Suzuki, Shinpei Yoshida, Haruaki Nobori, Takao Sanaki, Teruhisa Kato, Takao Shishido, William W Hall, Yasuko Orba, Akihiko Sato, Hirofumi Sawa

    Science translational medicine   15 ( 679 )   eabq4064   2022年11月

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    In parallel with vaccination, oral antiviral agents are highly anticipated to act as countermeasures for the treatment of the coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Oral antiviral medication demands not only high antiviral activity, but also target specificity, favorable oral bioavailability, and high metabolic stability. Although a large number of compounds have been identified as potential inhibitors of SARS-CoV-2 infection in vitro, few have proven to be effective in vivo. Here, we show that oral administration of S-217622 (ensitrelvir), an inhibitor of SARS-CoV-2 main protease (Mpro, also known as 3C-like protease), decreases viral load and ameliorates disease severity in SARS-CoV-2-infected hamsters. S-217622 inhibited viral proliferation at low nanomolar to sub-micromolar concentrations in cells. Oral administration of S-217622 demonstrated favorable pharmacokinetic properties and accelerated recovery from acute SARS-CoV-2 infection in hamster recipients. Moreover, S-217622 exerted antiviral activity against SARS-CoV-2 variants of concern (VOCs), including the highly pathogenic Delta variant and the recently emerged Omicron BA.5 and BA.2.75 variants. Overall, our study provides evidence that S-217622, an antiviral agent that is under evaluation in a phase 3 clinical trial (clinical trial registration no. jRCT2031210350), possesses remarkable antiviral potency and efficacy against SARS-CoV-2 and is a prospective oral therapeutic option for COVID-19.

    DOI: 10.1126/scitranslmed.abq4064

    PubMed

  • Virological characteristics of the SARS-CoV-2 Omicron BA.2 subvariants including BA.4 and BA.5 査読

    Izumi Kimura, Daichi Yamasoba, Tomokazu Tamura, Naganori Nao, Tateki Suzuki, Yoshitaka Oda, Shuya Mitoma, Jumpei Ito, Hesham Nasser, Jiri Zahradnik, Keiya Uriu, Shigeru Fujita, Yusuke Kosugi, Lei Wang, Masumi Tsuda, Mai Kishimoto, Hayato Ito, Rigel Suzuki, Ryo Shimizu, M.S.T. Monira Begum, Kumiko Yoshimatsu, Kanako Terakado Kimura, Jiei Sasaki, Kaori Sasaki-Tabata, Yuki Yamamoto, Tetsuharu Nagamoto, Jun Kanamune, Kouji Kobiyama, Hiroyuki Asakura, Mami Nagashima, Kenji Sadamasu, Kazuhisa Yoshimura, Kotaro Shirakawa, Akifumi Takaori-Kondo, Jin Kuramochi, Gideon Schreiber, Ken J. Ishii, Takao Hashiguchi, Terumasa Ikeda, Akatsuki Saito, Takasuke Fukuhara, Shinya Tanaka, Keita Matsuno, Kei Sato

    Cell   185 ( 21 )   3992 - 4007   2022年09月( ISSN:0092-8674

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    DOI: 10.1016/j.cell.2022.09.018

  • Isolation and characterization of an orthoreovirus from Indonesian fruit bats. 査読 国際共著

    Kittiya Intaruck, Yukari Itakura, Mai Kishimoto, Herman M Chambaro, Agus Setiyono, Ekowati Handharyani, Kentaro Uemura, Hayato Harima, Satoshi Taniguchi, Masayuki Saijo, Takashi Kimura, Yasuko Orba, Hirofumi Sawa, Michihito Sasaki

    Virology   575   10 - 19   2022年08月

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    Nelson Bay orthoreovirus (NBV) is an emerging bat-borne virus and causes respiratory tract infections in humans sporadically. Over the last two decades, several strains genetically related to NBV were isolated from humans and various bat species, predominantly in Southeast Asia (SEA), suggesting a high prevalence of the NBV species in this region. In this study, an orthoreovirus (ORV) belonging to the NBV species was isolated from Indonesian fruit bats' feces, tentatively named Paguyaman orthoreovirus (PgORV). Serological studies revealed that 81.2% (108/133) of Indonesian fruit bats sera had neutralizing antibodies against PgORV. Whole-genome sequencing and phylogenetic analysis of PgORV suggested the occurrence of past reassortments with other NBV strains isolated in SEA, indicating the dispersal and circulation of NBV species among bats in this region. Intranasal PgORV inoculation of laboratory mice caused severe pneumonia. Our study characterized PgORV's unique genetic background and highlighted the potential risk of PgORV-related diseases in Indonesia.

    DOI: 10.1016/j.virol.2022.08.003

    PubMed

  • A comprehensive list of the Bunyavirales replication promoters reveals a unique promoter structure in Nairoviridae differing from other virus families 査読

    Yutaro Neriya, Shohei Kojima, Arata Sakiyama, Mai Kishimoto, Takao Iketani, Tadashi Watanabe, Yuichi Abe, Hiroshi Shimoda, Keisuke Nakagawa, Takaaki Koma, Yusuke Matsumoto

    Scientific Reports   12 ( 1 )   13560   2022年08月

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    Abstract

    Members of the order Bunyavirales infect a wide variety of host species, including plants, animals and humans, and pose a threat to public health. Major families in this order have tri-segmented negative-sense RNA genomes, the 5′ and 3′ ends of which form complementary strands that serve as a replication promoter. Elucidation of the mechanisms by which viral polymerases recognize the promoter to initiate RNA synthesis is important for understanding viral replication and pathogenesis, and developing antivirals. A list of replication promoter configuration patterns may provide details on the differences in the replication mechanisms among bunyaviruses. By using public sequence data of all known bunyavirus species, we constructed a comprehensive list of the replication promoters comprising 40 nucleotides in both the 5′ and 3′ ends of the genome that form a specific complementary strand. Among tri-segmented bunyaviruses, members of the family Nairoviridae, including the highly pathogenic Crimean-Congo hemorrhagic fever virus, have evolved a GC-rich promoter structure differing from that of other families. The unique promoter structure might be related to the large genome size of the family Nairoviridae among bunyaviruses, and the large genome architecture might confer pathogenic advantages. The promoter list provided in this report is useful for predicting the virus family-specific replication mechanisms of bunyaviruses.

    DOI: 10.1038/s41598-022-17758-z

    その他URL: https://www.nature.com/articles/s41598-022-17758-z

  • Serological characterization of lineage II insect-specific flaviviruses compared with pathogenic mosquito-borne flaviviruses. 査読 国際共著

    Koshiro Tabata, Yukari Itakura, Shinsuke Toba, Kentaro Uemura, Mai Kishimoto, Michihito Sasaki, Jessica J Harrison, Akihiko Sato, William W Hall, Roy A Hall, Hirofumi Sawa, Yasuko Orba

    Biochemical and biophysical research communications   616   115 - 121   2022年08月

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    The genus Flavivirus includes pathogenic tick- and mosquito-borne flaviviruses as well as non-pathogenic insect-specific flaviviruses (ISFVs). Phylogenetic analysis based on whole amino acid sequences has indicated that lineage II ISFVs have similarities to pathogenic flaviviruses. In this study, we used reactive analysis with immune serum against Psorophora flavivirus (PSFV) as a lineage IIa ISFV, and Barkeji virus (BJV) as a lineage IIb ISFV, to evaluate the antigenic similarity among lineage IIa and IIb ISFVs, and pathogenic mosquito-borne flaviviruses (MBFVs). Binding and antibody-dependent enhancement assays showed that anti-PSFV sera had broad cross-reactivity with MBFV antigens, while anti-BJV sera had low cross-reactivity. Both of the lineage II ISFV antisera were rarely observed to neutralize MBFVs. These results suggest that lineage IIa ISFV PSFV has more antigenic similarity to MBFVs than lineage IIb ISFV BJV.

    DOI: 10.1016/j.bbrc.2022.05.080

    PubMed

  • Virological characteristics of the SARS-CoV-2 Omicron BA.2 spike. 査読

    Daichi Yamasoba, Izumi Kimura, Hesham Nasser, Yuhei Morioka, Naganori Nao, Jumpei Ito, Keiya Uriu, Masumi Tsuda, Jiri Zahradnik, Kotaro Shirakawa, Rigel Suzuki, Mai Kishimoto, Yusuke Kosugi, Kouji Kobiyama, Teppei Hara, Mako Toyoda, Yuri L Tanaka, Erika P Butlertanaka, Ryo Shimizu, Hayato Ito, Lei Wang, Yoshitaka Oda, Yasuko Orba, Michihito Sasaki, Kayoko Nagata, Kumiko Yoshimatsu, Hiroyuki Asakura, Mami Nagashima, Kenji Sadamasu, Kazuhisa Yoshimura, Jin Kuramochi, Motoaki Seki, Ryoji Fujiki, Atsushi Kaneda, Tadanaga Shimada, Taka-Aki Nakada, Seiichiro Sakao, Takuji Suzuki, Takamasa Ueno, Akifumi Takaori-Kondo, Ken J Ishii, Gideon Schreiber, Hirofumi Sawa, Akatsuki Saito, Takashi Irie, Shinya Tanaka, Keita Matsuno, Takasuke Fukuhara, Terumasa Ikeda, Kei Sato

    Cell   185 ( 12 )   2103 - 2115   2022年05月

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    Soon after the emergence and global spread of the SARS-CoV-2 Omicron lineage BA.1, another Omicron lineage, BA.2, began outcompeting BA.1. The results of statistical analysis showed that the effective reproduction number of BA.2 is 1.4-fold higher than that of BA.1. Neutralization experiments revealed that immunity induced by COVID vaccines widely administered to human populations is not effective against BA.2, similar to BA.1, and that the antigenicity of BA.2 is notably different from that of BA.1. Cell culture experiments showed that the BA.2 spike confers higher replication efficacy in human nasal epithelial cells and is more efficient in mediating syncytia formation than the BA.1 spike. Furthermore, infection experiments using hamsters indicated that the BA.2 spike-bearing virus is more pathogenic than the BA.1 spike-bearing virus. Altogether, the results of our multiscale investigations suggest that the risk of BA.2 to global health is potentially higher than that of BA.1.

    DOI: 10.1016/j.cell.2022.04.035

    PubMed

  • Attenuated fusogenicity and pathogenicity of SARS-CoV-2 Omicron variant 査読

    Rigel Suzuki, Daichi Yamasoba, Izumi Kimura, Lei Wang, Mai Kishimoto, Jumpei Ito, Yuhei Morioka, Naganori Nao, Hesham Nasser, Keiya Uriu, Yusuke Kosugi, Masumi Tsuda, Yasuko Orba, Michihito Sasaki, Ryo Shimizu, Ryoko Kawabata, Kumiko Yoshimatsu, Hiroyuki Asakura, Mami Nagashima, Kenji Sadamasu, Kazuhisa Yoshimura, Mai Suganami, Akiko Oide, Mika Chiba, Hayato Ito, Tomokazu Tamura, Kana Tsushima, Haruko Kubo, Zannatul Ferdous, Hiromi Mouri, Miki Iida, Keiko Kasahara, Koshiro Tabata, Mariko Ishizuka, Asako Shigeno, Kenzo Tokunaga, Seiya Ozono, Isao Yoshida, So Nakagawa, Jiaqi Wu, Miyoko Takahashi, Atsushi Kaneda, Motoaki Seki, Ryoji Fujiki, Bahityar Rahmutulla Nawai, Yutaka Suzuki, Yukie Kashima, Kazumi Abe, Kiyomi Imamura, Kotaro Shirakawa, Akifumi Takaori-Kondo, Yasuhiro Kazuma, Ryosuke Nomura, Yoshihito Horisawa, Kayoko Nagata, Yugo Kawai, Yohei Yanagida, Yusuke Tashiro, Otowa Takahashi, Kazuko Kitazato, Haruyo Hasebe, Chihiro Motozono, Mako Toyoda, Toong Seng Tan, Isaac Ngare, Takamasa Ueno, Akatsuki Saito, Erika P. Butlertanaka, Yuri L. Tanaka, Nanami Morizako, Hirofumi Sawa, Terumasa Ikeda, Takashi Irie, Keita Matsuno, Shinya Tanaka, Takasuke Fukuhara, Kei Sato

    Nature   603 ( 7902 )   700 - 705   2022年02月( ISSN:0028-0836

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    担当区分:筆頭著者   掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    <title>Abstract</title>The emergence of the Omicron variant of SARS-CoV-2 is an urgent global health concern<sup>1</sup>. In this study, our statistical modelling suggests that Omicron has spread more rapidly than the Delta variant in several countries including South Africa. Cell culture experiments showed Omicron to be less fusogenic than Delta and than an ancestral strain of SARS-CoV-2. Although the spike (S) protein of Delta is efficiently cleaved into two subunits, which facilitates cell–cell fusion<sup>2,3</sup>, the Omicron S protein was less efficiently cleaved compared to the S proteins of Delta and ancestral SARS-CoV-2. Furthermore, in a hamster model, Omicron showed decreased lung infectivity and was less pathogenic compared to Delta and ancestral SARS-CoV-2. Our multiscale investigations reveal the virological characteristics of Omicron, including rapid growth in the human population, lower fusogenicity and attenuated pathogenicity.

    DOI: 10.1038/s41586-022-04462-1

    その他URL: https://www.nature.com/articles/s41586-022-04462-1

  • Air-liquid interphase culture confers SARS-CoV-2 susceptibility to A549 alveolar epithelial cells. 査読 国際共著

    Michihito Sasaki, Mai Kishimoto, Yukari Itakura, Koshiro Tabata, Kittiya Intaruck, Kentaro Uemura, Shinsuke Toba, Takao Sanaki, Akihiko Sato, William W Hall, Yasuko Orba, Hirofumi Sawa

    Biochemical and biophysical research communications   577   146 - 151   2021年11月

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    The human lung cell A549 is susceptible to infection with a number of respiratory viruses. However, A549 cells are resistant to Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) infection in conventional submerged culture, and this would appear to be due to low expression levels of the SARS-CoV-2 entry receptor: angiotensin-converting enzyme-2 (ACE2). Here, we examined SARS-CoV-2 susceptibility to A549 cells after adaptation to air-liquid interface (ALI) culture. A549 cells in ALI culture yielded a layer of mucus on their apical surface, exhibited decreased expression levels of the proliferation marker KI-67 and intriguingly became susceptible to SARS-CoV-2 infection. We found that A549 cells increased the endogenous expression levels of ACE2 and TMPRSS2 following adaptation to ALI culture conditions. Camostat, a TMPRSS2 inhibitor, reduced SARS-CoV-2 infection in ALI-cultured A549 cells. These findings indicate that ALI culture switches the phenotype of A549 cells from resistance to susceptibility to SARS-CoV-2 infection through upregulation of ACE2 and TMPRSS2.

    DOI: 10.1016/j.bbrc.2021.09.015

    PubMed

  • SARS-CoV-2 Bearing a Mutation at the S1/S2 Cleavage Site Exhibits Attenuated Virulence and Confers Protective Immunity. 査読 国際共著

    Michihito Sasaki, Shinsuke Toba, Yukari Itakura, Herman M Chambaro, Mai Kishimoto, Koshiro Tabata, Kittiya Intaruck, Kentaro Uemura, Takao Sanaki, Akihiko Sato, William W Hall, Yasuko Orba, Hirofumi Sawa

    mBio   12 ( 4 )   e0141521   2021年08月

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) possesses a discriminative polybasic cleavage motif in its spike protein that is recognized by the host furin protease. Proteolytic cleavage activates the spike protein, thereby affecting both the cellular entry pathway and cell tropism of SARS-CoV-2. Here, we investigated the impact of the furin cleavage site on viral growth and pathogenesis using a hamster animal model infected with SARS-CoV-2 variants bearing mutations at the furin cleavage site (S gene mutants). In the airway tissues of hamsters, the S gene mutants exhibited low growth properties. In contrast to parental pathogenic SARS-CoV-2, hamsters infected with the S gene mutants showed no body weight loss and only a mild inflammatory response, thereby indicating the attenuated variant nature of S gene mutants. This transient infection was sufficient for inducing protective neutralizing antibodies that cross-react with different SARS-CoV-2 lineages. Consequently, hamsters inoculated with S gene mutants showed resistance to subsequent infection with both the parental strain and the currently emerging SARS-CoV-2 variants belonging to lineages B.1.1.7 and P.1. Taken together, our findings revealed that the loss of the furin cleavage site causes attenuation in the airway tissues of hamsters and highlighted the potential benefits of S gene mutants as potential immunogens. IMPORTANCE SARS-CoV-2 uses its spike protein to enter target cells. The spike protein is cleaved by a host protease, and this event facilitates viral entry and broadens cell tropism. In this study, we employed SARS-CoV-2 mutants lacking the S protein cleavage site and characterized their growth and pathogenicity using hamsters, a laboratory animal model for SARS-CoV-2 infection. These mutants exerted low pathogenicity but induced sufficient levels of neutralizing antibodies in hamsters, which protected hamsters from rechallenge with pathogenic clinical SARS-CoV-2 strains. These virus mutants may be used as protective immunogens against SARS-CoV-2 infection.

    DOI: 10.1128/mBio.01415-21

    PubMed

  • Host serine proteases TMPRSS2 and TMPRSS11D mediate proteolytic activation and trypsin-independent infection in group A rotaviruses. 査読 国際共著

    Michihito Sasaki, Yukari Itakura, Mai Kishimoto, Koshiro Tabata, Kentaro Uemura, Naoto Ito, Makoto Sugiyama, Christida E Wastika, Yasuko Orba, Hirofumi Sawa

    Journal of virology   95 ( 11 )   2021年03月

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    Group A rotaviruses (RVAs) are representative enteric virus species and major causes of diarrhea in humans and animals. The RVA virion is a triple-layered particle, and the outermost layer consists of the glycoprotein VP7 and spike protein VP4. To increase the infectivity of RVA, VP4 is proteolytically cleaved into VP5* and VP8* subunits by trypsin; and these subunits form a rigid spike structure on the virion surface. In this study, we investigated the growth of RVAs in cells transduced with type II transmembrane serine proteases (TTSPs), which cleave fusion proteins and promote infection by respiratory viruses, such as influenza viruses, paramyxoviruses, and coronaviruses. We identified TMPRSS2 and TMPRSS11D as host TTSPs that mediate trypsin-independent and multi-cycle infection by human and animal RVA strains. In vitro cleavage assays revealed that recombinant TMPRSS11D cleaved RVA VP4. We also found that TMPRSS2 and TMPRSS11D promote the infectious entry of immature RVA virions, but they could not activate nascent progeny virions in the late phase of infection. This observation differed from the TTSP-mediated activation process of paramyxoviruses, revealing the existence of virus species-specific activation processes in TTSPs. Our study provides new insights into the interaction between RVAs and host factors, and TTSP-transduced cells offer potential advantages for RVA research and development.ImportanceProteolytic cleavage of the viral VP4 protein is essential for virion maturation and infectivity in group A rotaviruses (RVAs). In cell culture, RVAs are propagated in culture medium supplemented with the exogenous protease trypsin, which cleaves VP4 and induces the maturation of progeny RVA virions. In this study, we demonstrated that the host proteases TMPRSS2 and TMPRSS11D mediate the trypsin-independent infection and growth of RVA. Our data revealed that the proteolytic activation of RVAs by TMPRSS2 and TMPRSS11D occurs at the viral entry step. Because TMPRSS2 and TMPRSS11D gene expression induced similar or higher levels of RVA growth as trypsin-supplemented culture, this approach offers potential advantages for RVA research and development.

    DOI: 10.1128/JVI.00398-21

    PubMed

  • Development of a one-run real-time PCR detection system for pathogens associated with porcine respiratory diseases. 査読

    Fujiko Sunaga, Shinobu Tsuchiaka, Mai Kishimoto, Hiroshi Aoki, Mari Kakinoki, Katsumasa Kure, Hanako Okumura, Maho Okumura, Atsushi Okumura, Makoto Nagai, Tsutomu Omatsu, Tetsuya Mizutani

    The Journal of veterinary medical science   82 ( 2 )   217 - 223   2020年02月

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    The etiology of Porcine respiratory disease complex is complicated by infections with multiple pathogens, and multiple infections increase the difficulty in identifying the causal pathogen. In this present study, we developed a detection system of microbes from porcine respiratory by using TaqMan real-time PCR (referred to as Dempo-PCR) to screen a broad range of pathogens associated with porcine respiratory diseases in a single run. We selected 17 porcine respiratory pathogens (Actinobacillus pleuropneumoniae, Boldetella bronchiseptica, Haemophilus parasuis, Pasteurella multocida, Pasteurella multocida toxin, Streptococcus suis, Mycoplasma hyopneumoniae, Mycoplasma hyorhinis, Mycoplasma hyosynovie, porcine circovirus 2, pseudorabies virus, porcine cytomegalovirus, swine influenza A virus, porcine reproductive and respiratory virus US strain, EU strain, porcine respiratory coronavirus and porcine hemagglutinating encephalomyelitis virus) as detection targets and designed novel specific primer-probe sets for seven of them. In sensitivity test by using standard curves from synthesized DNA, all primer-probe sets showed high sensitivity. However, porcine reproductive and respiratory virus is known to have a high frequency of genetic mutations, and the primer and probe sequences will need to be checked at a considerable frequency when performing Dempo-PCR from field samples. A total of 30 lung samples from swine showing respiratory symptoms on six farms were tested by the Dempo-PCR to validate the assay's clinical performance. As the results, 12 pathogens (5 virus and 7 bacteria) were detected and porcine reproductive and respiratory virus US strain, Mycoplasma hyorhinis, Haemophilus parasuis, and porcine cytomegalovirus were detected at high frequency. These results suggest that Dempo-PCR assay can be applied as a screening system with wide detection targets.

    DOI: 10.1292/jvms.19-0063

    PubMed

  • Rapid detection of pathogenic virus genome sequence from throat and nasal swab samples using an exhaustive gene amplification method. 査読

    Junji Hosokawa-Muto, Hiroki Sakai, Yukiko Sassa, Yoshihito Fujinami, Mai Kishimoto, Hiroaki Nakahara

    Forensic science, medicine, and pathology   15 ( 3 )   399 - 403   2019年09月

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    Rapid identification of pathogenic agents is important in response to the emergence of biocrime and bioterrorism, to facilitate appropriate confinement and treatment. As the rapid determination system of viral genome sequences (RDV method) using exhaustive gene amplification is useful for rapid identification, we examined whether this method could be applied to forensic samples. To detect pathogenic virus in a cat with suspected viral infections, fluid swab samples were applied to the RDV method. The following steps were performed: viral propagation, extraction of the viral genome, amplification of the first library, fragmentation of the library, amplification of the second library using non-specific primer sets, and direct sequencing of the amplicon. To confirm the viruses detected by this method, we performed conventional PCR using virus-specific primers. We detected pathogenic virus genome sequences from the swab samples and confirmed infection with these viruses. In addition, we directly detected a viral genome sequence from the nasal swab sample without the viral propagation step. The RDV method is infrequently used in forensic analysis. This method is practicable with equipment existing in a normal laboratory and is useful for rapid detection and identification of pathogenic viruses in forensic samples. This method would also be applicable to the detection of bacteria and fungi.

    DOI: 10.1007/s12024-019-00128-z

    PubMed

  • Dembo polymerase chain reaction technique for detection of bovine abortion, diarrhea, and respiratory disease complex infectious agents in potential vectors and reservoirs. 査読 国際共著

    Sayed Samim Rahpaya, Shinobu Tsuchiaka, Mai Kishimoto, Mami Oba, Yukie Katayama, Yuka Nunomura, Saki Kokawa, Takashi Kimura, Atsushi Kobayashi, Yumi Kirino, Tamaki Okabayashi, Nariaki Nonaka, Hirohisa Mekata, Hiroshi Aoki, Mai Shiokawa, Moeko Umetsu, Tatsushi Morita, Ayako Hasebe, Keiko Otsu, Tetsuo Asai, Tomohiro Yamaguchi, Shinji Makino, Yoshiteru Murata, Ahmad Jan Abi, Tsutomu Omatsu, Tetsuya Mizutani

    Journal of veterinary science   19 ( 3 )   350 - 357   2018年05月

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    DOI: 10.4142/jvs.2018.19.3.350

  • Genetic diversity and intergenogroup recombination events of sapoviruses detected from feces of pigs in Japan. 査読

    Moegi Kuroda, Tsuneyuki Masuda, Mika Ito, Yuki Naoi, Yen Hai Doan, Kei Haga, Shinobu Tsuchiaka, Mai Kishimoto, Kaori Sano, Tsutomu Omatsu, Yukie Katayama, Mami Oba, Hiroshi Aoki, Toru Ichimaru, Fujiko Sunaga, Itsuro Mukono, Hiroshi Yamasato, Junsuke Shirai, Kazuhiko Katayama, Tetsuya Mizutani, Tomoichiro Oka, Makoto Nagai

    Infection, genetics and evolution   55   209 - 217   2017年11月

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    DOI: 10.1016/j.meegid.2017.09.013

  • Complete genome analysis of porcine kobuviruses from the feces of pigs in Japan. 査読

    Masataka Akagami, Mika Ito, Kazutaka Niira, Moegi Kuroda, Tsuneyuki Masuda, Kei Haga, Shinobu Tsuchiaka, Yuki Naoi, Mai Kishimoto, Kaori Sano, Tsutomu Omatsu, Hiroshi Aoki, Yukie Katayama, Mami Oba, Tomoichiro Oka, Toru Ichimaru, Hiroshi Yamasato, Yoshinao Ouchi, Junsuke Shirai, Kazuhiko Katayama, Tetsuya Mizutani, Makoto Nagai

    Virus genes   53 ( 4 )   593 - 602   2017年08月

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    DOI: 10.1007/s11262-017-1464-9

  • Whole genome analysis of porcine astroviruses detected in Japanese pigs reveals genetic diversity and possible intra-genotypic recombination. 査読

    Mika Ito, Moegi Kuroda, Tsuneyuki Masuda, Masataka Akagami, Kei Haga, Shinobu Tsuchiaka, Mai Kishimoto, Yuki Naoi, Kaori Sano, Tsutomu Omatsu, Yukie Katayama, Mami Oba, Hiroshi Aoki, Toru Ichimaru, Itsuro Mukono, Yoshinao Ouchi, Hiroshi Yamasato, Junsuke Shirai, Kazuhiko Katayama, Tetsuya Mizutani, Makoto Nagai

    Infection, genetics and evolution   50   38 - 48   2017年06月

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    DOI: 10.1016/j.meegid.2017.02.008

  • Diversity in VP3, NSP3, and NSP4 of rotavirus B detected from Japanese cattle. 査読

    Michiko Hayashi-Miyamoto, Toshiaki Murakami, Fujiko Minami-Fukuda, Shinobu Tsuchiaka, Mai Kishimoto, Kaori Sano, Yuki Naoi, Keigo Asano, Toru Ichimaru, Kei Haga, Tsutomu Omatsu, Yukie Katayama, Mami Oba, Hiroshi Aoki, Junsuke Shirai, Motohiko Ishida, Kazuhiko Katayama, Tetsuya Mizutani, Makoto Nagai

    Infection, genetics and evolution   49   97 - 103   2017年04月

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    DOI: 10.1016/j.meegid.2017.01.003

  • Identification of a novel bovine enterovirus possessing highly divergent amino acid sequences in capsid protein. 査読

    Shinobu Tsuchiaka, Sayed Samim Rahpaya, Konosuke Otomaru, Hiroshi Aoki, Mai Kishimoto, Yuki Naoi, Tsutomu Omatsu, Kaori Sano, Sachiko Okazaki-Terashima, Yukie Katayama, Mami Oba, Makoto Nagai, Tetsuya Mizutani

    BMC microbiology   17 ( 1 )   18 - 18   2017年01月

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    DOI: 10.1186/s12866-016-0923-0

  • Identification of further diversity among posaviruses. 査読

    Kaori Sano, Yuki Naoi, Mai Kishimoto, Tsuneyuki Masuda, Hitomi Tanabe, Mika Ito, Kazutaka Niira, Kei Haga, Keigo Asano, Shinobu Tsuchiaka, Tsutomu Omatsu, Tetsuya Furuya, Yukie Katayama, Mami Oba, Yoshinao Ouchi, Hiroshi Yamasato, Motohiko Ishida, Junsuke Shirai, Kazuhiko Katayama, Tetsuya Mizutani, Makoto Nagai

    Archives of virology   161 ( 12 )   3541 - 3548   2016年12月

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    担当区分:筆頭著者   掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    DOI: 10.1007/s00705-016-3048-8

  • Whole genome sequences of Japanese porcine species C rotaviruses reveal a high diversity of genotypes of individual genes and will contribute to a comprehensive, generally accepted classification system. 査読

    Kazutaka Niira, Mika Ito, Tsuneyuki Masuda, Toshiya Saitou, Tadatsugu Abe, Satoshi Komoto, Mitsuo Sato, Hiroshi Yamasato, Mai Kishimoto, Yuki Naoi, Kaori Sano, Shinobu Tuchiaka, Takashi Okada, Tsutomu Omatsu, Tetsuya Furuya, Hiroshi Aoki, Yukie Katayama, Mami Oba, Junsuke Shirai, Koki Taniguchi, Tetsuya Mizutani, Makoto Nagai

    Infection, genetics and evolution   44   106 - 113   2016年10月

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    DOI: 10.1016/j.meegid.2016.06.041

  • Isolation of a sp. nov. Ljungan virus from wild birds in Japan. 査読

    Hiromichi Mitake, Yuji Fujii, Makoto Nagai, Naoto Ito, Kota Okadera, Kazuma Okada, Kento Nakagawa, Mai Kishimoto, Tetsuya Mizutani, Katsunori Okazaki, Yoshihiro Sakoda, Ayato Takada, Makoto Sugiyama

    The Journal of general virology   97 ( 8 )   1818 - 1822   2016年08月

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    DOI: 10.1099/jgv.0.000508

  • Characterization and phylogenetic analysis of a novel picornavirus from swine feces in Japan. 査読

    Yuki Naoi, Mai Kishimoto, Tsuneyuki Masuda, Mika Ito, Shinobu Tsuchiaka, Kaori Sano, Hiroshi Yamasato, Tsutomu Omatsu, Hiroshi Aoki, Tetsuya Furuya, Yukie Katayama, Mami Oba, Takashi Okada, Junsuke Shirai, Tetsuya Mizutani, Makoto Nagai

    Archives of virology   161 ( 6 )   1685 - 1690   2016年06月

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    担当区分:筆頭著者   掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    DOI: 10.1007/s00705-016-2834-7

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    岸本麻衣, 岸本麻衣, 梶原将大, 高田礼人, 大園誠也, 鈴木忠樹, 伊藤直人, 大場靖子, 澤洋文, 澤洋文, 佐々木道仁

    日本獣医学会学術集会講演要旨集   166th   2023年( ISSN:1347-8621

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  • SARS-CoV-2感染ハムスターを用いた経口抗ウイルス薬と抗炎症薬の併用治療効果の解析

    佐々木道仁, 杉達紀, 飯田俊, 平田雄一郎, 日下部伸治, 日下部伸治, 小西慧, 小西慧, 板倉友香里, 板倉友香里, 田畑耕史郎, 田畑耕史郎, 岸本麻衣, 岸本麻衣, 小林広子, 有泉拓馬, KITTIYA Intaruck, 登治謙, 鳥羽晋輔, 鳥羽晋輔, 佐藤彰彦, 佐藤彰彦, 佐藤彰彦, 松野啓太, 松野啓太, 山岸潤也, 山岸潤也, 鈴木忠樹, 大場靖子, 大場靖子, 澤洋文, 澤洋文, 澤洋文

    日本ウイルス学会学術集会プログラム・予稿集(Web)   70th   2023年

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  • SARS-CoV-2の感染を促進する宿主II型膜貫通型セリンプロテアーゼの同定

    岸本麻衣, 上村健太朗, 上村健太朗, 佐名木孝央, 佐名木孝央, 佐藤彰彦, 佐藤彰彦, 大場靖子, 澤洋文, 佐々木道仁

    日本分子生物学会年会プログラム・要旨集(Web)   44th   2021年

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  • Vero細胞での増殖中に出現する新型コロナウイルス変異株の解析

    佐々木道仁, 上村健太朗, 上村健太朗, 鳥羽晋輔, 鳥羽晋輔, 佐藤彰彦, 佐藤彰彦, 板倉友香里, INTARUCK Kittiya, 岸本麻衣, 田畑耕史郎, CHAMBARO Herman, 大場靖子, 大場靖子, 澤洋文, 澤洋文

    日本分子生物学会年会プログラム・要旨集(Web)   44th   2021年

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講演・口頭発表等

  • 多様な哺乳類および鳥類デルタウイルスから紐解くサテライト/ヘルパーウイルスと宿主動物の共進化機構 国内会議

    岸本麻衣、川崎純菜、北尾晃一、堀江真行

    第46回日本分子生物学会年会  2023年12月  日本分子生物学会

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    会議種別:ポスター発表  

    開催地:神戸  

  • 多様な哺乳類および鳥類デルタウイルスからサテライト/ヘルパーウイルスと宿主動物の共進化機構を紐解く 国内会議

    岸本麻衣、川崎純菜、北尾晃一、堀江真行

    NGS EXPO 2023  2023年11月  NGS EXPO実行委員会

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    会議種別:ポスター発表  

    開催地:大阪  

  • SARS-CoV-2非構造タンパク質のアミノ酸配列が関与するCOVID-19重症化メカニズム 国内会議

    岸本麻衣、鳥羽晋輔、上村健太朗、丸山優樹、日下部伸治、佐藤彰彦、大場靖子、澤洋文、佐々木道仁

    第70回日本ウイルス学会学術集会  2023年09月  日本ウイルス学会

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    会議種別:口頭発表(一般)  

    開催地:仙台  

  • ザンビアのエジプトルーセットオオコウモリおよびマストミスから分離されたロタウイルスAの性状解析 国内会議

    岸本麻衣、梶原将大、高田礼人、大園誠也、鈴木忠樹 、伊藤直人、大場靖子、澤洋文、佐々木道仁

    第166回日本獣医学会学術集会  2023年09月  日本獣医学会

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    会議種別:口頭発表(一般)  

    開催地:オンライン  

  • SARS-CoV-2非構造タンパク質によって規定されるCOVID-19重症化の分子機構 国内会議

    岸本麻衣、鳥羽晋輔、上村健太朗、丸山優樹、日下部伸治、佐藤彰彦、大場靖子、澤洋文、佐々木道仁

    第45回日本分子生物学会年会  2022年11月  日本分子生物学会

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    会議種別:ポスター発表  

    開催地:幕張  

  • ウイルス分離先行型スクリーニングにより分離された野生動物由来ロタウイルスAの性状解析 国内会議

    岸本麻衣、梶原将大、高田礼人、伊藤直人、大場靖子、澤洋文、佐々木道仁

    第69回日本ウイルス学会学術集会  2022年11月  日本ウイルス学会

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    会議種別:口頭発表(一般)  

    開催地:長崎  

  • SARS-CoV-2の感染を促進する宿主II型膜貫通型セリンプロテアーゼの同定 国内会議

    岸本麻衣、上村健太朗、佐名木孝央、佐藤彰彦、大場靖子、澤洋文、佐々木道仁

    第44回日本分子生物学会年会  2021年12月  日本分子生物学会

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    会議種別:ポスター発表  

    開催地:横浜  

  • SARS-CoV-2と宿主II型膜貫通型セリンプロテアーゼ(TTSP)の相互作用 国内会議

    岸本麻衣、上村健太朗、佐名木孝央、佐藤彰彦、大場靖子、澤洋文、佐々木道仁

    第68回日本ウイルス学会学術集会  2021年11月  日本ウイルス学会

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    会議種別:ポスター発表  

    開催地:神戸  

  • ザンビアに生息する野生げっ歯類動物における脳心筋炎ウイルス(EMCV)の疫学調査 国際共著 国内会議

    岸本麻衣、Bernard M. Hang’ombe、大場靖子、澤洋文、佐々木道仁

    第164回日本獣医学会学術集会  2021年09月  日本獣医学会

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    会議種別:口頭発表(一般)  

    開催地:オンライン  

  • Isolation and characterization of encephalomyocarditis virus (EMCV) from Mastomys natalensis, a potential reservoir rodent in Zambia 国際共著 国際会議

    Mai Kishimoto

    The 8th SaSSOH, 2020  2020年09月  SaSSOH commitee, Hokkaido University

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    会議種別:口頭発表(一般)  

    開催地:Sapporo, Japan  

  • 次世代シーケンサーによる死後経過した感染臓器試料中のウイルス遺伝子の検出

    武藤 淳二[細川], 岸本 麻衣, 藤浪 良仁, 中原 弘明

    日本法医学雑誌  2019年05月  (NPO)日本法医学会

▼全件表示

科研費

  • プロテアーゼ恒常発現系を利用した高効率ウイルス分離法の開発

    特別研究員奨励費  2023年04月

  • プロテアーゼ恒常発現系を利用した高効率ウイルス分離法の開発

    特別研究員奨励費  2021年04月

担当授業科目

  • 獣医微生物・免疫学実習

    2023年度   週間授業   大学

  • 獣医微生物・免疫学実習

    2023年度   週間授業   大学

学外での担当授業科目

  • 国際感染症防疫学

    2023年07月
    機関名:東京農工大学

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    科目区分:大学院専門科目  国名:日本国