Updated on 2024/04/26

写真a

 
TSUKAMOTO MASAYA
 
Organization
Graduate School of Veterinary Science Department of Veterinary Science Lecturer
School of Veterinary Science Department of Veterinary Science
Title
Lecturer
Affiliation
Institute of Veterinary Science

Position

  • Graduate School of Veterinary Science Department of Veterinary Science 

    Lecturer  2024.04 - Now

  • School of Veterinary Science Department of Veterinary Science 

    Lecturer  2024.04 - Now

Degree

  • 博士(獣医学) ( Osaka Prefecture University )

Research Areas

  • Life Science / Embryonic medicine and pediatrics

  • Life Science / Developmental biology

  • Life Science / Veterinary medical science  / Veterinary regenerative medicine

Research Interests

  • iPS細胞

  • Embryonic stem cells

  • Veterinary regenerative medicine

  • 小腸オルガノイド

  • Regenerative medicine

Professional Memberships

  • The Japanese Society of Veterinary Medicine

  • The Japanese Society for Regenerative Medicine

  • International Society for Stem Cell Research

Awards

  • 学会賞

    塚本雅也

    2019.02   日本獣医再生医療学会   獣医再生医療へ応用可能なイヌiPS細胞作製方法の確立

  • 学長顕彰

    2019   大阪府立大学  

Job Career (off-campus)

  • National Center for Child Health and Development

    2023.10 - 2024.03

  • 独立行政法人日本学術振興会 特別研究員PD

    2022.04 - 2024.03

  • 国立成育医療研究センター   再生医療センター   共同研究員

    2022.04 - 2023.10

  • 独立行政法人日本学術振興会 特別研究員DC1

    2018.04 - 2022.03

Papers

  • Canine induced pluripotent stem cells can be successfully maintained in weekend-free culture systems

    KIMURA Kazuto, NAGAKURA Hiroya, TSUKAMOTO Masaya, YOSHIDA Takumi, SUGISAKI Hiroko, SHISHIDA Kohei, TACHI Yuta, SHIMASAKI Shoko, SUGIURA Kikuya, HATOYA Shingo

    Journal of Veterinary Medical Science   86 ( 3 )   247 - 257   2024( ISSN:09167250

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:Domestic journal  

    <p>Canine induced pluripotent stem cells (ciPSCs) can provide useful insights into novel therapies in both veterinary and medical fields. However, limited accessibility to the present culture medium and requirement of considerable time, effort, and cost for routine ciPSC maintenance restrict advancement in ciPSC research. In addition, it is unknown whether ciPSC culture conditions influence differentiation propensity. We investigated the availability of the common human pluripotent stem cells (hPSCs) culture systems for ciPSC maintenance and the differentiation propensities of the ciPSCs maintained in these culture systems. StemFlex and mTeSR Plus supported PSC-like colony formation and pluripotency markers expression in ciPSCs even after five passages. Additionally, ciPSCs were maintained under weekend-free culture conditions with a stable growth rate, pluripotency marker expression, and differentiation abilities using vitronectin (VTN-N) and Geltrex. Following maintenance of spontaneously differentiated ciPSCs under various conditions by embryoid body formation, there were few differences in the differentiation propensities of ciPSCs among the tested culture conditions. Thus, ciPSCs were successfully cultured under weekend-free conditions for ciPSC maintenance using StemFlex or mTeSR Plus with VTN-N or Geltrex. The present study offers simpler and more effort-, time-, and cost-saving options for ciPSC culture systems, which may lead to further development in research using ciPSCs.</p>

    DOI: 10.1292/jvms.23-0422

    PubMed

  • Generation of canine induced pluripotent stem cells under feeder-free conditions using Sendai virus vector encoding six canine reprogramming factors Reviewed

    Masaya Tsukamoto, Kazuto Kimura, Takumi Yoshida, Miyuu Tanaka, Mitsuru Kuwamura, Taro Ayabe, Genki Ishihara, Kei Watanabe, Mika Okada, Minoru Iijima, Mahito Nakanishi, Hidenori Akutsu, Kikuya Sugiura, Shingo Hatoya

    Stem Cell Reports   19 ( 1 )   141 - 157   2023.12( ISSN:2213-6711

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.stemcr.2023.11.010

    PubMed

  • Canine induced pluripotent stem cells efficiently differentiate into definitive endoderm in 3D cell culture conditions using high-dose activin A Reviewed

    Masaya Tsukamoto, Kazuto Kimura, Takumi Yoshida, Kikuya Sugiura, Shingo Hatoya

    Regenerative Therapy   21   502 - 510   2022.12( ISSN:2352-3204

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.reth.2022.10.002

    PubMed

  • Effects of the preservation medium and storage duration of domestic cat ovaries on the maturational and developmental competence of oocytes in vitro

    68 ( 2 )   160 - 164   2022.04( ISSN:0916-8818

  • Canine induced pluripotent stem cell maintenance under feeder‐free and chemically‐defined conditions Reviewed

    Kazuto Kimura, Masaya tsukamoto, Takumi Yoshida, Miyuu Tanaka, Mitsuru Kuwamura, Manami Ohtaka, Ken Nishimura, Mahito Nakanishi, Kikuya Sugiura, Shingo Hatoya

    Molecular Reproduction and Development   88 ( 6 )   395 - 404   2021.05( ISSN:1040-452X

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/mrd.23478

  • Efficient Reprogramming of Canine Peripheral Blood Mononuclear Cells into Induced Pluripotent Stem Cells

    Kazuto Kimura, Masaya tsukamoto, Miyuu Tanaka, Mitsuru Kuwamura, Manami Ohtaka, Ken Nishimura, Mahito Nakanishi, Kikuya Sugiura, Shingo Hatoya

    Stem Cells and Development   30 ( 2 )   79 - 90   2021.01( ISSN:1547-3287

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/scd.2020.0084

  • Generation of Footprint‐Free Canine Induced Pluripotent Stem Cells from Peripheral Blood Mononuclear Cells Using Sendai Virus Vector Reviewed

    Masaya tsukamoto, Kazuto Kimura, Miyuu Tanaka, Mitsuru Kuwamura, Manami Ohtaka, Mahito Nakanishi, Kikuya Sugiura, Shingo Hatoya

    Molecular Reproduction and Development   87 ( 6 )   663 - 665   2020.05( ISSN:1040-452X

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/mrd.23349

  • Manipulation of the tumor microenvironment by cytokine gene transfection enhances dendritic cell‐based immunotherapy Reviewed

    Daluthgamage Patsy Himali Wijesekera, Eiji Yuba, Nadeeka Harshini De Silva, Shun‐ichi Watanabe, Masaya tsukamoto, Chihiro Ichida, Takeshi Izawa, Kazuyuki Itoh, Ryoji Kanegi, Shingo Hatoya, Jyoji Yamate, Toshio Inaba, Kikuya Sugiura

    FASEB BioAdvances   2 ( 1 )   5 - 17   2020.01( ISSN:2573-9832

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1096/fba.2019-00052

  • Development of feline embryos produced by Piezo-actuated intracytoplasmic sperm injection of elongated spermatids

    Tsujimoto Yasunori, Fujiki Kana, Alam Md Emtiaj, Tsukamoto Masaya, Azuma Rika, Kanegi Ryoji, Anzai Masayuki, Inaba Toshio, Sugiura Kikuya, Hatoya Shingo

    The Journal of Reproduction and Development   65 ( 3 )   245 - 250   2019.06( ISSN:0916-8818

  • Generation of Footprint-Free Canine Induced Pluripotent Stem Cells Using Auto-Erasable Sendai Virus Vector Reviewed

    Masaya tsukamoto, Toshiya Nishimura, Kyohei Yodoe, Ryoji Kanegi, Yasunori Tsujimoto, Md Emtiaj Alam, Mizuki Kuramochi, Mitsuru Kuwamura, Manami Ohtaka, Ken Nishimura, Mahito Nakanishi, Toshio Inaba, Kikuya Sugiura, Shingo Hatoya

    Stem Cells and Development   27 ( 22 )   1577 - 1586   2018.11

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    International / domestic magazine:International journal  

    DOI: https://doi.org/10.1089/scd.2018.0084

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Books and Other Publications

  • 動物実験代替法とNew approach methodologiesの開発・利用動向

    塚本雅也, 阿久津英憲( Role: Contributor ,  高機能化腸オルガノイドの動物実験代替法への応用性)

    シーエムシー出版  2023.09  ( ISBN:9784781317472

     More details

    Total pages:x, 296p  

    CiNii Books

Presentations

  • 輸血への応用に向けた赤芽球由来イヌiPS細胞の作製と赤血球への分化誘導

    木村和人, 塚本雅也, 田中美有, 桑村充, 綾部太郎, 石原玄基, 中西真人, 杉浦喜久弥, 鳩谷晋吾

    第19回 日本獣医内科学アカデミー学術大会  2023.02 

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    Presentation type:Oral presentation (general)  

  • イヌiPS細胞の安定的かつ効率的なフィーダーフリー培養条件の検討

    長倉京哉, 塚本雅也, 木村和人, 杉浦喜久弥, 鳩谷晋吾

    第165回日本獣医学会学術集会  2022.09 

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    Presentation type:Oral presentation (general)  

  • 末梢血単核球を用いた効率的なイヌiPS細胞の樹立および大量培養

    第164回日本獣医学会学術集会  2021.09 

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    Presentation type:Oral presentation (general)  

  • イヌiPS細胞から間葉系幹細胞の誘導の試み

    塚本 雅也, 川端 千晶, 木村 和人, 吉田 拓海, 杉浦 喜久弥, 鳩谷 晋吾

    第164回日本獣医学会学術集会  2021.09 

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    Presentation type:Oral presentation (general)  

  • Differentiation of canine induced pluripotent stem cells into definitive endoderm.

    Masaya Tsukamoto, Kazuto Kimura, Kikuya Sugiura, Shingo Hatoya

    International Society for Stem Cell Research 2021 VIRTUAL The Global Stem Cell Event  2021.06 

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    Presentation type:Poster presentation  

  • Chemically-defined and Feeder-free Maintenance of Canine Induced Pluripotent Stem Cells

    Kazuto Kimura, Masaya Tsukamoto, Manami Ohtaka, Ken Nishimura, Mahito Nakanishi, Kikuya Sugiura, Shingo Hatoya

    International Society for Stem Cell Research 2021 VIRTUAL The Global Stem Cell Event  2021.06 

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    Presentation type:Poster presentation  

  • Reprogramming of canine embryonic fibroblasts to Primed and Naïve like pluripotent states.

    Tsukamoto Masaya, Kimura Kazuto, Kanegi Ryoji, Ohtaka Manami, Nishimura Ken, Nakanishi Mahito, Inaba Toshio, Sugiura Kikuya, Hatoya Shingo

    International Society for Stem Cell Research 2020 VIRTUAL The Global Stem Cell Event  2020.06 

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    Presentation type:Poster presentation  

  • Efficient Generation of Canine Induced Pluripotent Stem Cells from Peripheral Blood Mononuclear Cells Using a Small Molecule Cocktail

    Kazuto Kimura, Masaya Tsukamoto, Manami Ohtaka, Ken Nishimura, Mahito Nakanishi, Kikuya Sugiura, Shingo Hatoya

    International Society for Stem Cell Research 2020 VIRTUAL The Global Stem Cell Event  2020.06 

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    Presentation type:Poster presentation  

  • Approaches to the Generation of Feline Induced Pluripotent Stem Cells Using Sendai Virus Vectors

    Shingo Hatoya, Shota Inoue, Kazuto Kimura, Masaya Tsukamoto, Manami Ohtaka, Ken Nishimura, Mahito Nakanishi, Kikuya Sugiura

    International Society for Stem Cell Research 2020 VIRTUAL The Global Stem Cell Event  2020.06 

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    Presentation type:Poster presentation  

  • 末梢血単核球を利用したイヌiPS細胞作製の試み

    塚本雅也, 木村和人, 大高真奈美, 西村健, 中西真人, 杉浦喜久弥, 鳩谷晋吾

    第19回日本再生医療学会  2020.05 

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    Presentation type:Poster presentation  

  • 末梢血単核球からのイヌiPS細胞作製に向けた培養条件の検討

    木村和人, 塚本雅也, 大高真奈美, 西村健, 中西真人, 杉浦喜久弥, 鳩谷晋吾

    第19回日本再生医療学会  2020.05 

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    Presentation type:Poster presentation  

  • 獣医再生医療へ応用可能なイヌiPS細胞作製方法の確立

    塚本雅也

    第14回日本獣医再生医療学会  2019.02 

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    Presentation type:Oral presentation (general)  

  • 治療や病態解明へ応用可能なイヌiPS細胞の作製 Domestic conference

    塚本雅也, 西村俊哉, 金城綾二, 倉持瑞樹, 桑村充, 大高真奈美, 西村健, 中西真人, 稲葉俊夫, 杉浦喜久弥, 鳩谷晋吾

    第161回日本獣医学会学術集会  2018.09 

  • Induction of Transgene Free Canine Induced Pluripotent Stem Cells by Using Sendai Virus Vectors. International conference

    Tsukamoto Masaya, Kanegi Ryoji, Nishimura Toshiya, Ohtaka, Manami, Nishimura Ken, Nakanishi Mahito, Inaba Toshio, Sugiura Kikuya, Hatoya Shingo

    5th Tissue Engineering and Regenerative Medicine International Society World Congress  2018.09 

  • 臨床応用へ向けたイヌiPS細胞の効率的な作製 Domestic conference

    塚本雅也, 金城綾二, 大高真奈美, 西村健, 中西真人, 稲葉俊夫, 杉浦喜久弥, 鳩谷晋吾

    第13回日本獣医再生医療学会  2018.02 

  • Primed型およびNaive型イヌiPS細胞の効率的な作製 Domestic conference

    塚本雅也, 鳩谷晋吾, 金城綾二, 大高真奈美, 西村健, 中西真人, 稲葉俊夫, 杉浦喜久弥

    第160回日本獣医学会学術集会  2017.09 

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Grant-in-Aid for Scientific Research

  • ミニ腸による新規ex vivo壊死性腸炎モデル開発と病態分子機序の研究

    Grant-in-Aid for Early-Career Scientists  2023.04

  • M細胞を標的とするヒトミニ腸モデル構築と小腸粘膜免疫応答の分子機構に関する研究

    Grant-in-Aid for JSPS Fellows  2023.03

  • イヌiPS細胞を用いたin vitro炎症性腸疾患モデルの病態解明・創薬への応用

    Grant-in-Aid for JSPS Fellows  2019.04

Charge of on-campus class subject

  • 獣医内科学実習

    2024   Practical Training   Undergraduate

  • 獣医臨床病理学

    2024   Weekly class   Undergraduate

  • 獣医血液病学

    2024   Weekly class   Undergraduate

  • 臨床基礎実習

    2024   Practical Training   Undergraduate

Media Coverage

  • イヌの尿由来細胞からのiPS細胞安定作製に成功 Internet

    文永堂出版 JVM NEWS  2023.12

  • 大阪公立大など、イヌの尿中細胞からフィーダーフリーでiPS細胞の作製に成功 Internet

    日経バイオテク web  2023.12

  • 世界初! イヌの尿由来細胞からのiPS細胞安定作製に成功

    大阪公立大学  2023.12

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    SDGs:

  • 犬の尿からiPS細胞作製 高効率で作製 Internet

    Yahoo!ニュース  2023.12

  • フィーダーフリー実現 大阪公立大学など イヌiPS細胞作製で Newspaper, magazine

    化学工業日報  3面  2023.12

  • イヌの尿からiPS細胞作製 手法確立、研究進展に期待―大阪公立大など Internet

    時事通信 web  2023.12

  • イヌの尿からiPS細胞効率作製 将来の治療応用に期待 Internet

    共同通信 web  2023.12

  • イヌの尿からiPS細胞を高効率で作製 大阪公立大など世界初の手法 獣医療での再生医療の促進に期待 Internet

    読売テレビニュース web  2023.12

  • イヌの尿からiPS細胞 作製手法確立、進展に期待 Newspaper, magazine

    公明新聞  3面  2023.12

  • イヌの尿からiPS細胞 Newspaper, magazine

    読売新聞  夕刊8面  2023.12

  • イヌのiPS細胞作製 大阪公立大 尿由来細胞など利用 Newspaper, magazine

    日刊工業新聞  29面  2023.12

  • イヌiPS細胞効率作製 大阪公立大など、尿を使用 Newspaper, magazine

    日経新聞  夕刊11面  2023.12

  • New stem cell therapy in dogs -- a breakthrough in veterinary medicine Internet

    American Association for the Advancement of Science  2021.02

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