Development of a one-run real-time PCR detection system for pathogens associated with porcine respiratory diseases

SUNAGA Fujiko, TSUCHIAKA Shinobu, KISHIMOTO Mai, AOKI Hiroshi, KAKINOKI Mari, KURE Katsumasa, OKUMURA Hanako, OKUMURA Maho, OKUMURA Atsushi, NAGAI Makoto, OMATSU Tsutomu, MIZUTANI Tetsuya

Journal of Veterinary Medical Science   82 ( 2 )   217 - 223   2020.02（ ISSN:0916-7250 ） （ eISSN:13477439 ）

A novel defective recombinant porcine enterovirus G virus carrying a porcine torovirus papain-like cysteine protease gene and a putative anti-apoptosis gene in place of viral structural protein genes. Reviewed

Imai R, Nagai M, Oba M, Sakaguchi S, Ujike M, Kimura R, Kida M, Masuda T, Kuroda M, Wen R, Li K, Katayama Y, Naoi Y, Tsuchiaka S, Omatsu T, Yamazato H, Makino S, Mizutani T

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases   75   103975   2019.11（ ISSN:1567-1348 ）

Dembo polymerase chain reaction technique for detection of bovine abortion, diarrhea, and respiratory disease complex infectious agents in potential vectors and reservoirs. Reviewed

Rahpaya SS, Tsuchiaka S, Kishimoto M, Oba M, Katayama Y, Nunomura Y, Kokawa S, Kimura T, Kobayashi A, Kirino Y, Okabayashi T, Nonaka N, Mekata H, Aoki H, Shiokawa M, Umetsu M, Morita T, Hasebe A, Otsu K, Asai T, Yamaguchi T, Makino S, Murata Y, Abi AJ, Omatsu T, Mizutani T

Journal of veterinary science   19 ( 3 )   350 - 357   2018.05（ ISSN:1229-845X ）

A new comprehensive method for detection of livestock-related pathogenic viruses using a target enrichment system. Reviewed

Oba M, Tsuchiaka S, Omatsu T, Katayama Y, Otomaru K, Hirata T, Aoki H, Murata Y, Makino S, Nagai M, Mizutani T

Biochemical and biophysical research communications   495 ( 2 )   1871 - 1877   2018.01（ ISSN:0006-291X ）

Genetic diversity and recombination of enterovirus G strains in Japanese pigs: High prevalence of strains carrying a papain-like cysteine protease sequence in the enterovirus G population. Reviewed

Tsuchiaka S, Naoi Y, Imai R, Masuda T, Ito M, Akagami M, Ouchi Y, Ishii K, Sakaguchi S, Omatsu T, Katayama Y, Oba M, Shirai J, Satani Y, Takashima Y, Taniguchi Y, Takasu M, Madarame H, Sunaga F, Aoki H, Makino S, Mizutani T, Nagai M

PloS one   13 ( 1 )   e0190819   2018

Characteristics of Staphylococcal Enterotoxin A Production and Growth of Staphylococcus aureus in Shaking and Stationary Cultures. Reviewed

Elahi S, Tsuchiaka S, Mizutani T, Fujikawa H

Biocontrol science   23 ( 4 )   207 - 214   2018（ ISSN:1342-4815 ）

Discovery of genome of an immunodeficiency-associated virus-like virus from pig feces in Japan Reviewed

Oba M.

Japanese Journal of Veterinary Research   66 ( 1 )   53 - 56   2018（ ISSN:00471917 ）

Genetic diversity and intergenogroup recombination events of sapoviruses detected from feces of pigs in Japan. Reviewed

Kuroda M, Masuda T, Ito M, Naoi Y, Doan YH, Haga K, Tsuchiaka S, Kishimoto M, Sano K, Omatsu T, Katayama Y, Oba M, Aoki H, Ichimaru T, Sunaga F, Mukono I, Yamasato H, Shirai J, Katayama K, Mizutani T, Oka T, Nagai M

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases   55   209 - 217   2017.11（ ISSN:1567-1348 ）

Discovery of fur seal feces-associated circular DNA virus in swine feces in Japan. Reviewed

Oba M, Katayama Y, Naoi Y, Tsuchiaka S, Omatsu T, Okumura A, Nagai M, Mizutani T

The Journal of veterinary medical science   79 ( 10 )   1664 - 1666   2017.10（ ISSN:0916-7250 ）

Molecular characteristics and prevalence of small ruminant lentiviruses in goats in Japan. Reviewed

Kokawa S, Oba M, Hirata T, Tamaki S, Omura M, Tsuchiaka S, Nagai M, Omatsu T, Mizutani T

Archives of virology   162 ( 10 )   3007 - 3015   2017.10（ ISSN:0304-8608 ）

Complete genome analysis of porcine kobuviruses from the feces of pigs in Japan. Reviewed

Akagami M, Ito M, Niira K, Kuroda M, Masuda T, Haga K, Tsuchiaka S, Naoi Y, Kishimoto M, Sano K, Omatsu T, Aoki H, Katayama Y, Oba M, Oka T, Ichimaru T, Yamasato H, Ouchi Y, Shirai J, Katayama K, Mizutani T, Nagai M

Virus genes   53 ( 4 )   593 - 602   2017.08（ ISSN:0920-8569 ）

First isolation and characterization of pteropine orthoreoviruses in fruit bats in the Philippines. Reviewed

Taniguchi S, Maeda K, Horimoto T, Masangkay JS, Puentespina R Jr, Alvarez J, Eres E, Cosico E, Nagata N, Egawa K, Singh H, Fukuma A, Yoshikawa T, Tani H, Fukushi S, Tsuchiaka S, Omatsu T, Mizutani T, Une Y, Yoshikawa Y, Shimojima M, Saijo M, Kyuwa S

Archives of virology   162 ( 6 )   1529 - 1539   2017.06（ ISSN:0304-8608 ）

Whole genome analysis of porcine astroviruses detected in Japanese pigs reveals genetic diversity and possible intra-genotypic recombination. Reviewed

Ito M, Kuroda M, Masuda T, Akagami M, Haga K, Tsuchiaka S, Kishimoto M, Naoi Y, Sano K, Omatsu T, Katayama Y, Oba M, Aoki H, Ichimaru T, Mukono I, Ouchi Y, Yamasato H, Shirai J, Katayama K, Mizutani T, Nagai M

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases   50   38 - 48   2017.06（ ISSN:1567-1348 ）

Diversity in VP3, NSP3, and NSP4 of rotavirus B detected from Japanese cattle. Reviewed

Hayashi-Miyamoto M, Murakami T, Minami-Fukuda F, Tsuchiaka S, Kishimoto M, Sano K, Naoi Y, Asano K, Ichimaru T, Haga K, Omatsu T, Katayama Y, Oba M, Aoki H, Shirai J, Ishida M, Katayama K, Mizutani T, Nagai M

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases   49   97 - 103   2017.04（ ISSN:1567-1348 ）

Development of a one-run real-time PCR detection system for pathogens associated with bovine respiratory disease complex. Reviewed

Kishimoto M, Tsuchiaka S, Rahpaya SS, Hasebe A, Otsu K, Sugimura S, Kobayashi S, Komatsu N, Nagai M, Omatsu T, Naoi Y, Sano K, Okazaki-Terashima S, Oba M, Katayama Y, Sato R, Asai T, Mizutani T

The Journal of veterinary medical science   79 ( 3 )   517 - 523   2017.03（ ISSN:0916-7250 ）

Identification of a novel bovine enterovirus possessing highly divergent amino acid sequences in capsid protein. Reviewed

Tsuchiaka S, Rahpaya SS, Otomaru K, Aoki H, Kishimoto M, Naoi Y, Omatsu T, Sano K, Okazaki-Terashima S, Katayama Y, Oba M, Nagai M, Mizutani T

BMC microbiology   17 ( 1 )   18   2017.01

Identification of further diversity among posaviruses. Reviewed

Sano K, Naoi Y, Kishimoto M, Masuda T, Tanabe H, Ito M, Niira K, Haga K, Asano K, Tsuchiaka S, Omatsu T, Furuya T, Katayama Y, Oba M, Ouchi Y, Yamasato H, Ishida M, Shirai J, Katayama K, Mizutani T, Nagai M

Archives of virology   161 ( 12 )   3541 - 3548   2016.12（ ISSN:0304-8608 ）

Detection of Campylobacter jejuni in rectal swab samples from Rousettus amplexicaudatus in the Philippines. Reviewed

Hatta Y, Omatsu T, Tsuchiaka S, Katayama Y, Taniguchi S, Masangkay JS, Puentespina R Jr, Eres E, Cosico E, Une Y, Yoshikawa Y, Maeda K, Kyuwa S, Mizutani T

The Journal of veterinary medical science   78 ( 8 )   1347 - 50   2016.09（ ISSN:0916-7250 ）

Activation of c-Jun N-terminal kinase by Akabane virus is required for apoptosis Reviewed

Mitomo S.

Research in Veterinary Science   107   147 - 151   2016.08（ ISSN:00345288 ）

Pathological Features and Pathogenesis of the Endomyocardial Form of Restrictive Cardiomyopathy in Cats Reviewed

Kimura Y.

Journal of Comparative Pathology   155 ( 2-3 )   190 - 198   2016.08（ ISSN:00219975 ）

Characterization and phylogenetic analysis of a novel picornavirus from swine feces in Japan. Reviewed

Naoi Y, Kishimoto M, Masuda T, Ito M, Tsuchiaka S, Sano K, Yamasato H, Omatsu T, Aoki H, Furuya T, Katayama Y, Oba M, Okada T, Shirai J, Mizutani T, Nagai M

Archives of virology   161 ( 6 )   1685 - 90   2016.06（ ISSN:0304-8608 ）

Detection of Japanese eel endothelial cells-infecting virus in Anguilla japonica elvers. Reviewed

Okazaki S, Yasumoto S, Koyama S, Tsuchiaka S, Naoi Y, Omatsu T, Ono S, Mizutani T

The Journal of veterinary medical science   78 ( 4 )   705 - 7   2016.05（ ISSN:0916-7250 ）

Whole genome analysis of Japanese bovine toroviruses reveals natural recombination between porcine and bovine toroviruses. Reviewed

Ito M, Tsuchiaka S, Naoi Y, Otomaru K, Sato M, Masuda T, Haga K, Oka T, Yamasato H, Omatsu T, Sugimura S, Aoki H, Furuya T, Katayama Y, Oba M, Shirai J, Katayama K, Mizutani T, Nagai M

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases   38   90 - 95   2016.03（ ISSN:1567-1348 ）

Development of a novel detection system for microbes from bovine diarrhea by real-time PCR. Reviewed

Tsuchiaka S, Masuda T, Sugimura S, Kobayashi S, Komatsu N, Nagai M, Omatsu T, Furuya T, Oba M, Katayama Y, Kanda S, Yokoyama T, Mizutani T

The Journal of veterinary medical science   78 ( 3 )   383 - 9   2016.03（ ISSN:0916-7250 ）

Development of one-step real-time reverse transcriptase-PCR-based assays for the rapid and simultaneous detection of four viruses causing porcine diarrhea. Reviewed

Masuda T, Tsuchiaka S, Ashiba T, Yamasato H, Fukunari K, Omatsu T, Furuya T, Shirai J, Mizutani T, Nagai M

The Japanese journal of veterinary research   64 ( 1 )   5 - 14   2016.02（ ISSN:0047-1917 ）

Detection of novel kobu-like viruses in Japanese black cattle in Japan. Reviewed

Otomaru K, Naoi Y, Haga K, Omatsu T, Uto T, Koizumi M, Masuda T, Yamasato H, Takai H, Aoki H, Tsuchiaka S, Sano K, Okazaki S, Katayama Y, Oba M, Furuya T, Shirai J, Katayama K, Mizutani T, Nagai M

The Journal of veterinary medical science   78 ( 2 )   321 - 4   2016.02（ ISSN:0916-7250 ）

Detection of Japanese Eel Endothelial Cells-infecting Virus (JEECV) in mature Japanese Eel anguilla japonica caught from their spawning area Reviewed

Okazaki-Terashima S.

Fish Pathology   51 ( 2 )   64 - 66   2016（ ISSN:0388788X ）

Identification and complete genome analysis of a novel bovine picornavirus in Japan. Reviewed

Nagai M, Omatsu T, Aoki H, Kaku Y, Belsham GJ, Haga K, Naoi Y, Sano K, Umetsu M, Shiokawa M, Tsuchiaka S, Furuya T, Okazaki S, Katayama Y, Oba M, Shirai J, Katayama K, Mizutani T

Virus research   210   205 - 12   2015.12（ ISSN:0168-1702 ）

Full genome analysis of bovine astrovirus from fecal samples of cattle in Japan: identification of possible interspecies transmission of bovine astrovirus. Reviewed

Nagai M, Omatsu T, Aoki H, Otomaru K, Uto T, Koizumi M, Minami-Fukuda F, Takai H, Murakami T, Masuda T, Yamasato H, Shiokawa M, Tsuchiaka S, Naoi Y, Sano K, Okazaki S, Katayama Y, Oba M, Furuya T, Shirai J, Mizutani T

Archives of virology   160 ( 10 )   2491 - 501   2015.10（ ISSN:0304-8608 ）

Detection of Japanese eel endothelial cells-infecting virus (JEECV) in the Japanese eel Anguilla japonica (Temminck & Schlegel), living in natural habitats Reviewed

Okazaki S.

Journal of Fish Diseases   38 ( 9 )   849 - 852   2015.09（ ISSN:01407775 ）

H2 genotypes of G4P[6], G5P[7], and G9[23] porcine rotaviruses show super-short RNA electropherotypes. Reviewed

Nagai M, Shimada S, Fujii Y, Moriyama H, Oba M, Katayama Y, Tsuchiaka S, Okazaki S, Omatsu T, Furuya T, Koyama S, Shirai J, Katayama K, Mizutani T

Veterinary microbiology   176 ( 3-4 )   250 - 6   2015.04（ ISSN:0378-1135 ）

Identification of novel bovine group A rotavirus G15P[14] strain from epizootic diarrhea of adult cows by de novo sequencing using a next-generation sequencer. Reviewed

Masuda T, Nagai M, Yamasato H, Tsuchiaka S, Okazaki S, Katayama Y, Oba M, Nishiura N, Sassa Y, Omatsu T, Furuya T, Koyama S, Shirai J, Taniguchi K, Fujii Y, Todaka R, Katayama K, Mizutani T

Veterinary microbiology   171 ( 1-2 )   66 - 73   2014.06（ ISSN:0378-1135 ）

High-molecular-weight polyethylene glycol enhances hypothermic storage of feline kidney cells. Reviewed

Katayama M, Tsuchiaka S, Motegi T, Miyazaki M, Yamashita T, Shimamura S, Okamura Y, Uzuka Y

The Journal of veterinary medical science   76 ( 6 )   923 - 6   2014.06（ ISSN:0916-7250 ）

Detection of enterovirus genome sequence from diarrheal feces of goat. Reviewed

Omatsu T, Tsuchiaka S, Hirata T, Shiroma Y, Okazaki S, Katayama Y, Oba M, Nishiura N, Sassa Y, Furuya T, Nagai M, Ochiai H, Tamaki S, Mizutani T

Virus genes   48 ( 3 )   550 - 2   2014.06（ ISSN:0920-8569 ）

Detection of bovine group a rotavirus using rapid antigen detection kits, rt-PCR and next-generation DNA sequencing. Reviewed

Minami-Fukuda F, Nagai M, Takai H, Murakami T, Ozawa T, Tsuchiaka S, Okazaki S, Katayama Y, Oba M, Nishiura N, Sassa Y, Omatsu T, Furuya T, Koyama S, Shirai J, Tsunemitsu H, Fujii Y, Katayama K, Mizutani T

The Journal of veterinary medical science   75 ( 12 )   1651 - 5   2013.12（ ISSN:0916-7250 ）

獣医繁殖学実習

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生産動物臨床学

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臨床基礎実習

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大動物臨床学特論

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大動物臨床A

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大動物診療実習

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臨床基礎実習

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畜産学実習

2024   Intensive lecture   Undergraduate

畜産学実習

2024   Intensive lecture   Undergraduate

生産動物臨床実習

2024   Intensive lecture   Undergraduate

臨床基礎実習

2024   Weekly class   Undergraduate

獣医繁殖学実習

2024   Weekly class   Undergraduate

生産動物臨床学

2024   Weekly class   Undergraduate

産業動物生産獣医療

2024   Weekly class   Undergraduate

獣医繁殖学実習

獣医体験演習

大動物診療実習

大動物臨床学特論

大動物臨床A

伴侶動物診療学特論

臨床基礎実習

生産動物臨床実習

産業動物生産獣医療

臨床基礎実習

産業動物生産獣医療

大動物臨床A

大動物臨床学特論

大動物診療実習

獣医繁殖学実習

生産動物臨床学

生産動物臨床実習

畜産学実習

畜産学実習

臨床基礎実習