Updated on 2025/04/18

写真a

 
SHIRAGAKI KUMPEI
 
Organization
Graduate School of Agriculture Department of Agricultural Biology Assistant Professor
School of Agriculture Department of Agricultural Biology
Title
Assistant Professor
Affiliation
Institute of Agriculture

Position

  • Graduate School of Agriculture Department of Agricultural Biology 

    Assistant Professor  2025.04 - Now

  • School of Agriculture Department of Agricultural Biology 

    Assistant Professor  2025.04 - Now

Degree

  • 博士(応用生命科学) ( Osaka Prefecture University )

Research Areas

  • Environmental Science/Agriculture Science / Science in plant genetics and breeding

Research Interests

  • 植物育種

  • 生殖隔離

  • 系統解析

  • 資源循環

Papers

  • Abnormalities in juvenile-to-adult transition are associated with hybrid weakness in chili pepper (Capsicum) Reviewed

    Kumpei Shiragaki, Shuji Yokoi, Takahiro Tezuka

    Plant Breeding   143 ( 3 )   403 - 411   2024.06( ISSN:0179-9541 ( eISSN:1439-0523

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)  

    Weakness was observed in the F1 hybrids of crosses between Capsicum annuum × Capsicum chinense or C. annuum × Capsicum frutescens. This phenomenon is morphologically characterized by the cessation of new leaf development approximately 30 days after germination (DAG). We wondered why this phenomenon appeared in the middle of growth and studied the relationship between Capsicum hybrid weakness and the juvenile-to-adult (JA) phase transition. Traits that changed during the early vegetative phase in both parents were the petiole/leaf length ratio, number of leaf lateral veins and stomata, colour of epidermal cells in leaves stained with toluidine blue and expression of miR156 and miR172. Based on the changes observed during the early vegetative phase, C. annuum and C. chinense were transferred to the adult phase 28 DAG. However, the F1 hybrids did not show characteristics of the adult phase at any time point. Furthermore, the phenotypes of hybrid weakness were observed at approximately 35 DAG, when F1 hybrids were presumed to reach the JA transition phase. It has been suggested that the JA transition is involved in Capsicum hybrid weakness.

    DOI: 10.1111/pbr.13174

  • Understanding and overcoming hybrid lethality in seed and seedling stages as barriers to hybridization and gene flow Reviewed

    Hai He, Kumpei Shiragaki, Takahiro Tezuka

    Frontiers in Plant Science   14   2023.07( eISSN:1664-462X

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    Publishing type:Research paper (scientific journal)  

    Hybrid lethality is a type of reproductive isolation barrier observed in two developmental stages, hybrid embryos (hybrid seeds) and hybrid seedlings. Hybrid lethality has been reported in many plant species and limits distant hybridization breeding including interspecific and intergeneric hybridization, which increases genetic diversity and contributes to produce new germplasm for agricultural purposes. Recent studies have provided molecular and genetic evidence suggesting that underlying causes of hybrid lethality involve epistatic interaction of one or more loci, as hypothesized by the Bateson–Dobzhansky–Muller model, and effective ploidy or endosperm balance number. In this review, we focus on the similarities and differences between hybrid seed lethality and hybrid seedling lethality, as well as methods of recovering seed/seedling activity to circumvent hybrid lethality. Current knowledge summarized in our article will provides new insights into the mechanisms of hybrid lethality and effective methods for circumventing hybrid lethality.

    DOI: 10.3389/fpls.2023.1219417

  • Capsicum annuum with causal allele of hybrid weakness is prevalent in Asia Reviewed

    Kumpei Shiragaki, Shonosuke Seko, Shuji Yokoi, Takahiro Tezuka

    PLOS ONE   17 ( 7 )   e0271091 - e0271091   2022.07( eISSN:1932-6203

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)  

    Reproductive isolation, including hybrid weakness, plays an important role in the formation of species. Hybrid weakness in Capsicum, the cessation of plant growth, is caused by two complementary dominant genes, A from C. chinense or C. frutescens and B from C. annuum. In the present study, we surveyed whether 94 C. annuum accessions had B or b alleles by crossing with C. chinense having the A allele. Of the 94 C. annuum accessions, five had the B allele, three of which were native to Latin America and two were native to Asia. When combined with previous studies, the percentage of B carriers was 41% in Japan, 13% in Asia excluding Japan, 6% in Latin America, and 0% in Europe and Africa. In addition, 48 accessions of C. annuum from various countries were subjected to SSR analysis. Clades with high percentages of B-carriers were formed in the phylogenetic trees. In the principal coordinate analysis, most B-carriers were localized in a single group, although the group also included b-carriers. Based on these results, we presumed that the B allele was acquired in some C. annuum lines in Latin America, and B-carriers were introduced to the world during the Age of Discovery, as along with the b-carriers.

    DOI: 10.1371/journal.pone.0271091

  • Two Nicotiana occidentalis accessions enable gene identification for Type II hybrid lethality by the cross to N. sylvestris Reviewed

    Kenji Kawaguchi, Yuichiro Ohya, Maho Maekawa, Takahiro Iizuka, Akira Hasegawa, Kumpei Shiragaki, Hai He, Masayuki Oda, Toshinobu Morikawa, Shuji Yokoi, Takahiro Tezuka

    Scientific Reports   11 ( 1 )   17093 - 17093   2021.12( eISSN:2045-2322

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    <title>Abstract</title>Hybrid lethality, meaning the death of F<sub>1</sub> hybrid seedlings, has been observed in many plant species, including <italic>Nicotiana</italic>. Previously, we have revealed that hybrids of the selected <italic>Nicotiana occidentalis</italic> accession and <italic>N. tabacum</italic>, an allotetraploid with S and T genomes, exhibited lethality characterized by the fading of shoot color. The lethality was suggested to be controlled by alleles of loci on the S and T genomes derived from <italic>N. sylvestris</italic> and <italic>N. tomentosiformis</italic>, respectively. Here, we extended the analysis of hybrid lethality using other two accessions of <italic>N. occidentalis</italic> identified from the five tested accessions. The two accessions were crossed with <italic>N. tabacum</italic> and its two progenitors, <italic>N. sylvestris</italic> and <italic>N. tomentosiformis</italic>. After crosses with <italic>N. tabacum</italic>, the two <italic>N. occidentalis</italic> accessions yielded inviable hybrid seedlings whose lethality was characterized by the fading of shoot color, but only the T genome of <italic>N. tabacum</italic> was responsible for hybrid lethality. Genetic analysis indicated that first-mentioned <italic>N. occidentalis</italic> accession carries a single gene causing hybrid lethality by allelic interaction with the S genome.

    DOI: 10.1038/s41598-021-96482-6

    PubMed

    Other URL: https://www.nature.com/articles/s41598-021-96482-6

  • Temperature-dependent sugar accumulation in interspecific Capsicum F1 plants showing hybrid weakness Reviewed

    Kumpei Shiragaki, Hajime Furukawa, Shuji Yokoi, Takahiro Tezuka

    Journal of Plant Research   134 ( 6 )   1199 - 1211   2021.11( ISSN:0918-9440 ( eISSN:1618-0860

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)   International / domestic magazine:Domestic journal  

    In plants, F1 hybrids showing hybrid weakness exhibit weaker growth than their parents. The phenotypes of hybrid weakness are often suppressed at certain temperatures. However, it is unclear whether hybrid weakness in Capsicum annuum × C. chinense is temperature-dependent or not. Our study showed that Capsicum hybrid weakness was suppressed at 30 and 35 °C and was induced at 15, 20, and 25 °C. Moreover, we investigated the time course of hybrid weakness in cell death, metabolite content, and gene expression in leaves of plants transferred to 20 °C after growing at 30 °C for 21 days. The expression of pathogen defense-related genes was upregulated at 1 day after transfer to 20 °C (DAT). Cell death was detected at 7 DAT, plant growth had almost stopped since 14 DAT, and sugars were accumulated at 42 DAT in hybrid plants. The study revealed that some sugar transporter genes, which had been upregulated since 7 DAT, were involved in sugar accumulation in Capsicum hybrid weakness. Thus, our results demonstrated that gene expression changes occur first, followed by physiological and morphological changes after induction of hybrid weakness. These responses observed in this study in Capsicum hybrid weakness are likely to be owed to plant defense responses-like reactions.

    DOI: 10.1007/s10265-021-01340-1

    PubMed

    Other URL: https://link.springer.com/article/10.1007/s10265-021-01340-1/fulltext.html

  • Genetic Mapping of the HLA1 Locus Causing Hybrid Lethality in Nicotiana Interspecific Hybrids Reviewed

    Takahiro Tezuka, Naoto Kitamura, Sae Imagawa, Akira Hasegawa, Kumpei Shiragaki, Hai He, Masanori Yanase, Yoshiyuki Ogata, Toshinobu Morikawa, Shuji Yokoi

    Plants   10 ( 10 )   2062   2021.09( eISSN:2223-7747

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    Publishing type:Research paper (scientific journal)  

    Hybrid lethality, a postzygotic mechanism of reproductive isolation, is a phenomenon that causes the death of F1 hybrid seedlings. Hybrid lethality is generally caused by the epistatic interaction of two or more loci. In the genus Nicotiana, N. debneyi has the dominant allele Hla1-1 at the HLA1 locus that causes hybrid lethality in F1 hybrid seedlings by interaction with N. tabacum allele(s). Here, we mapped the HLA1 locus using the F2 population segregating for the Hla1-1 allele derived from the interspecific cross between N. debneyi and N. fragrans. To map HLA1, several DNA markers including random amplified polymorphic DNA, amplified fragment length polymorphism, and simple sequence repeat markers, were used. Additionally, DNA markers were developed based on disease resistance gene homologs identified from the genome sequence of N. benthamiana. Linkage analysis revealed that HLA1 was located between two cleaved amplified polymorphic sequence markers Nb14-CAPS and NbRGH1-CAPS at a distance of 10.8 and 10.9 cM, respectively. The distance between these markers was equivalent to a 682 kb interval in the genome sequence of N. benthamiana.

    DOI: 10.3390/plants10102062

  • Chemical and microbiological evaluation of vermicompost made from school food waste in Japan Reviewed

    Shumpei Hashimoto, Mai Furuya, Xiaodong You, Genki Wanibuchi, Hayato Tokumoto, Motoaki Tojo, Kumpei Shiragaki

    Japan Agricultural Research Quarterly: JARQ   55 ( 3 )   225 - 232   2021.07( ISSN:0021-3551 ( eISSN:2185-8896

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    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)  

    Vermicomposting technology is an environmentally friendly, sustainable, and low-cost tool used to convert agronomical and food waste into manure, facilitated by the decomposition and digestion by earthworms. In this study, we evaluated the chemical properties and microbial diversity of vermicompost (VC) derived from school lunch waste, which has long been a serious problem in many countries including Japan. The results revealed that 18-week-old VC promoted the root elongation of plants, and also showed a higher germination index (GI) and higher cation exchange capacity (CEC), indicating that vermicompost made from school food waste is an acceptable manure. As a result of the microbiome analysis by the sequencing of 16S ribosomal DNA (rDNA) using next generation sequencing (NGS), higher ratios of Bacillus, Pseudomonas, and Paenibacillus species, which may include beneficial bacteria for plant growth, were detected in VC than in the control compost (CC). These results demonstrate the significance of vermicompost in utilizing waste from school lunch, suggesting the possibility of implementing a waste recycling system that leads to reducing and recycling food waste in schools

    DOI: 10.6090/jarq.55.225

  • Phylogenetic Analysis and Molecular Diversity of Capsicum Based on rDNA-ITS Region Reviewed

    Kumpei Shiragaki, Shuji Yokoi, Takahiro Tezuka

    Horticulturae   6 ( 4 )   87 - 13   2020.11( eISSN:2311-7524

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)  

    The genus Capsicum is comprised of 5 domesticated and more than 30 wild species. The region of nuclear ribosomal DNA internal transcribed spacers (rDNA-ITS) has widely been used for species identification, but has rarely been used in Capsicum. In this study, the evaluation of genetic diversity and a phylogenetic analysis were conducted using rDNA-ITS of 28 Capsicum accessions, including five domesticated and two wild species. We surveyed six conventional keys of domesticated species and another five traits in Capsicum accessions. Specific morphological characteristics were found in C. annuum, C. baccatum, and C.pubescens. Three subclones of each accession were sequenced, and rDNA-ITS polymorphisms were detected in all accessions excluding C. annuum, suggesting that incomplete concerted evolution occurred in rDNA-ITS of Capsicum. The genetic diversity was evaluated using nucleotide polymorphism and diversity. C. annuum had the lowest genetic diversity of all species in this study. The phylogenetic tree formed a species-specific clade for C. annuum, C. baccatum, and C. pubescens. The C. chinense clade existed in the C. frutescens clade, implying that it was a cultivated variant of C. frutescens. C. chacoense likely belonged to the C. baccatum complex according to its morphologic and genetic features. This study indicated that the rDNA-ITS region can be used for simple identification of domesticated Capsicum species.

    DOI: 10.3390/horticulturae6040087

  • Phenylalanine ammonia-lyase and phenolic compounds are related to hybrid lethality in the cross <i>Nicotiana suaveolens</i>×<i>N. tabacum</i> Reviewed

    Kumpei Shiragaki, Rie Nakamura, Shigeki Nomura, Hai He, Tetsuya Yamada, Wataru Marubashi, Masayuki Oda, Takahiro Tezuka

    Plant Biotechnology   37 ( 3 )   327 - 333   2020.09( ISSN:1342-4580 ( eISSN:1347-6114

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)  

    Hybrid lethality observed in hybrid seedlings between Nicotiana suaveolens and N. tabacum is characterized by browning, initially of the hypocotyls and eventually of entire seedlings. We investigated the mechanism underlying this browning of tissues. A phenylalanine ammonia-lyase (PAL) gene codes an enzyme involved in a pathway producing phenolic compounds related to the browning of plant tissues. The expression of PAL rapidly increased with the induction of hybrid lethality. Phenolic compounds were observed to be accumulated in whole parts of hybrid seedlings. Treatment of hybrid seedlings with L-2-aminooxy-3-phenylpropionic acid (AOPP), an inhibitor for PAL, suppressed browning and decreased the phenolic content of hybrid seedlings. Although programmed cell death (PCD) was involved in hybrid lethality, AOPP treatment also suppressed cell death and enhanced the growth of hybrid seedlings. These results indicated that PAL is involved in hybrid lethality, and phenolic compounds could be the cause of hybrid lethality-associated tissue browning.

    DOI: 10.5511/plantbiotechnology.20.0606a

  • A hypersensitive response-like reaction is involved in hybrid weakness in F<sub>1</sub> plants of the cross <i>Capsicum annuum</i> × <i>Capsicum chinense</i> Reviewed

    Kumpei Shiragaki, Shuji Yokoi, Takahiro Tezuka

    Breeding Science   70 ( 4 )   430 - 437   2020.09( ISSN:1344-7610 ( eISSN:1347-3735

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)   International / domestic magazine:Domestic journal  

    Hybrid weakness in Capsicum is characterized by the termination of leaf differentiation after the development of several leaves. F1 plants in some crosses between Capsicum annuum and Capsicum chinense show weakness; this phenomenon has not been investigated in detail since first reported. In the present study, we characterized morphologically and physiologically hybrid weakness in Capsicum. F1 plants did not show weaker growth than their parents 20 days after germination (DAG), but at 40 DAG, the hybrid weakness phenotype was evidenced by almost complete arrest of new leaf formation, delayed increase in plant height, and reduced upper internode length. The shoot apical meristem (SAM) of F1 plants exhibited delayed development and an abnormal structure characterized by a flat shape and the presence of fuzzy cell layers on the surface. These abnormal SAMs of F1 plants may lead to dwarfism. Dead cells and accumulation of H2O2 were visually detected in leaves of F1 plants, and cell death was considered to be programmed, as it was accompanied by internucleosomal fragmentation of DNA. The expression of immunity marker genes PR1 and PR2 was upregulated in leaves of F1 plants. These results suggest that a hypersensitive response-like reaction is involved in Capsicum hybrid weakness.

    DOI: 10.1270/jsbbs.19137

    PubMed

  • HWA1- and HWA2-mediated hybrid weakness in rice involves cell death, reactive oxygen species accumulation, and disease resistance-related gene upregulation Reviewed

    Kumpei Shiragaki, Takahiro Iizuka, Katsuyuki Ichitani, Tsutomu Kuboyama, Toshinobu Morikawa, Masayuki Oda, Takahiro Tezuka

    Plants   8 ( 11 )   450   2019.10

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)  

    DOI: 10.3390/plants8110450

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MISC

  • Temperature sensitivity and sugar accumulation in hybrid weakness in the cross between Capsicum annuum and C. chinense

    白柿薫平, 古川一, 横井修司, 手塚孝弘

    育種学研究   22   2020( ISSN:1344-7629

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  • Phenotypic analysis of hybrid weakness in the cross between Capsicum annuum and C. chinense

    白柿薫平, 横井修司, 手塚孝弘

    育種学研究   22   2020( ISSN:1344-7629

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  • Phylogeographic analysis of hybrid weakness in Capsicum

    白柿薫平, 横井修司, 手塚孝弘

    日本進化学会大会プログラム・講演要旨集(Web)   22nd   2020

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  • 形態形質とrDNAのITS領域を用いたトウガラシ属栽培種5種の系統解析

    白柿薫平, 横井修司, 手塚孝弘

    育種学研究   20   2018( ISSN:1344-7629

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  • イネにおけるHWA1とHWA2およびHWC1とHWC2による雑種弱勢の比較解析

    白柿薫平, 佐原敏基, 一谷勝之, 久保山勉, 松村篤, 森川利信, 手塚孝弘

    育種学研究   19   2017( ISSN:1344-7629

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  • HWA1とHWA2によって生じるイネ雑種弱勢の遺伝子発現解析

    白柿薫平, 一谷勝之, 久保山勉, 簗瀬雅則, 森川利信, 手塚孝弘

    育種学研究   17   2015( ISSN:1344-7629

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