Updated on 2023/03/30

写真a

 
TACHIBANA Akira
 
Organization
Graduate School of Engineering Division of Science and Engineering for Materials, Chemistry and Biology Professor
School of Engineering Department of Chemistry and Bioengineering
Title
Professor
Affiliation
Institute of Engineering

Position

  • Graduate School of Engineering Division of Science and Engineering for Materials, Chemistry and Biology 

    Professor  2022.04 - Now

  • School of Engineering Department of Chemistry and Bioengineering 

    Professor  2022.04 - Now

Degree

  • Doctor of Science ( Others )

Research Areas

  • Life Science / Molecular biology

  • Life Science / Biomaterials  / Medical Organism Engineering and Material Science of Organism

  • Life Science / Biomedical engineering  / Medical Organism Engineering and Material Science of Organism

  • Life Science / Biomaterials  / Medical Organism Engineering and Material Science of Organism

  • Life Science / Biomedical engineering  / Medical Organism Engineering and Material Science of Organism

Research Career

  • Controlling cell growth and differentiation on patterned scaffolds

    growth factor, cell differentiation, patterned scaffold  Individual

    1900.04 

  • high efficient siRNA/miRNA sturctures

    RNAi, siRNA, miRNA  Individual

    1900.04 

  • in vitro selection of aptamer for new targets

    aptamer, nucleic acids, in vitro selection  Individual

    1900.04 

  • Functional Development of Keratin Protein

    Keratin  Joint Research in Organization

    1900.04 

Professional Memberships

  • The Society for Biotechnology, Japan

      Domestic

  • THE MOLECULAR BIOLOGY SOCIETY OF JAPAN

      Domestic

  • The Society for Biotechnology, Japan

  • THE MOLECULAR BIOLOGY SOCIETY OF JAPAN

Committee Memberships (off-campus)

  • 和文誌編集委員   日本生物工学会  

    2007.06 - 2011.05 

  • 生物工学会教育委員   生物工学会教育委員会  

    2015.06 - Now 

  • 生物工学会関西支部委員   生物工学会関西支部会  

    2015.06 - Now 

Awards

  • 第70回日本生物工学会大会 トピックス

    地白和樹、立花亮

    2018   環状RNAを用いたmiRNAとその前駆体の阻害

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    Country:Japan

  • 第70回日本生物工学会大会 トピックス

    地白和樹, 立花亮

    2018   環状RNAを用いたmiRNAとその前駆体の阻害

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    Country:Japan

  • 第65回日本生物工学会大会 トピックス

    伊田寛之、立花亮、田辺利住

    2013   一本鎖DNA領域の付加によるRNAサイレンシング効果の向上

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    Country:Japan

  • 第65回日本生物工学会大会 トピックス

    伊田寛之, 立花亮, 田辺利住

    2013   一本鎖DNA領域の付加によるRNAサイレンシング効果の向上

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    Country:Japan

  • 第64回日本生物工学会大会 トピックス

    米田善紀、伊田寛之、斉藤智、立花亮、田辺利住

    2012   非修飾DNAによるmiRNA阻害剤LidNA

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    Country:Japan

  • 第64回日本生物工学会大会 トピックス

    米田善紀, 伊田寛之, 斉藤智, 立花亮, 田辺利住

    2012   非修飾DNAによるmiRNA阻害剤LidNA

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    Country:Japan

  • 大阪市立大学教職員提案 奨励賞

    2009  

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    Country:Japan

  • 大阪市立大学教職員提案 奨励賞

    2009  

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    Country:Japan

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Job Career (off-campus)

  • Osaka City University   Graduate School of Engineering Applied Chemistry and Bioengineering Course

    1998.04 - Now

Education

  • Osaka City University   Doctor's Course  

    - 1994

  • Osaka City University    

    - 1988

Papers

  • Establishing a ready biodegradability test system using OxiTop<sup>®</sup> to evaluate chemical fate in a realistic environment

    Takekoshi Saki, Takano Kotaro, Matoba Yoshihide, Mukumoto Makiko, Tachibana Akira

    Journal of Pesticide Science   47 ( 1 )   35 - 42   2022.05( ISSN:1348589X

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    <p>The purpose of this study is to propose the use of OxiTop<sup>®</sup> for measuring biochemical oxygen demand (BOD) under the Japanese Chemical Substances Control Law in order to properly evaluate chemical fate in a real environment. In our previous study, the biodegradation of test chemicals was accelerated by both adsorbing the chemical to silica gel with chloroform and increasing the medium volume from 300 to 3900 mL in the OECD 301F test using a coulometer. However, the biodegradability of these chemicals could not be evaluated based on BOD due to chloroform residue in the silica gel, or the medium volume could not be increased further due to the oven size of the coulometer. In this study, we established an evaluation system using OxiTop<sup>®</sup> based on BOD by increasing the medium volume to 9000 mL. Based on triplicate testing, increasing the medium volume accelerated biodegradation and decreased variation in BOD.</p>

    DOI: 10.1584/jpestics.d21-046

    CiNii Article

  • Investigation of OECD 301F ready biodegradability test to evaluate chemical fate in a realistic environment

    Takekoshi Saki, Takano Kotaro, Matoba Yoshihide, Sato Masayuki, Tachibana Akira

    Journal of Pesticide Science   46 ( 2 )   143 - 151   2021.05( ISSN:1348589X

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    <p>The OECD 301F ready biodegradability test has been approved for use under the Japanese Chemical Substances Control Law since 2018. This test uses activated sludge obtained from a sewage treatment plant instead of the standard activated sludge used for the 301C test. In addition, the test is allowed to add an inert support or emulsifying agent, and/or to change the volume of the test medium. In this study, we first confirmed that the standard sludge had lower biodegradation activities than the sludge taken from a sewage treatment plant. Second, we showed that biodegradation percentages were increased by adding suitable amounts of silica gel or Tween 80. Third, we found that the biodegradations were accelerated by only increasing the medium volume under the conditions that concentrations of chemical, silica gel, and sludge were held constant. These findings are expected to contribute to the appropriate evaluation of chemical fate in a realistic environment.</p>

    DOI: 10.1584/jpestics.d20-050

    CiNii Article

  • Uniform straw-like cell architecture for three-dimensional cell-cell communication assay Reviewed

    Yusaku Inubushi, Yoshiki Sakaguchi , Akira Tachibana

    Biosci Biotechnol Biochem   84 ( 8 )   1681 - 1684   2020.08

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • 三次元細胞間コミュニケーション測定のための均一な細胞構造体(Uniform straw-like cell architecture for three-dimensional cell-cell communication assay)

    Inubushi Yusaku, Sakaguchi Yoshiki, Tachibana Akira

    Bioscience, Biotechnology, and Biochemistry   84 ( 8 )   1681 - 1684   2020.08( ISSN:0916-8451

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    シリコンチューブの中央に縫合糸を入れ、培養細胞を流し込み、遠心分離して、中空の細胞塊を作成した。縫合糸を抜いて、中心部に脂肪細胞を加えて培養し、三次元細胞間コミュニケーションを測定するシステムを作成した。成熟脂肪細胞は複数の細胞からなる自発的な細胞塊を形成できなかったが、この方法でストロー状の癌細胞と脂肪細胞の構造体を作成できた。これらの細胞の存在はGFP標識細胞により確認された。MCF7細胞と成熟脂肪細胞を培養したところ、4日目で剥がれたが、細胞-細胞の接着が弱まったのではなく、脂肪酸の放出のような細胞間のコミュニケーションの結果と考えられた。このシステムで乳癌由来MCF7細胞と成熟脂肪細胞を培養した。その結果、癌細胞の脂肪酸トランスロカーゼ(CD36)と癌細胞の増殖と転移を促進するFOXM1の発現が促進された。本システムで三次元の細胞間コミュニケーションを解析できると考えられた。

  • 人工股関節全置換術後(前外側アプローチ)患者の入院中の独歩獲得に影響する因子

    川上 友希, 立花 亮, 森本 鉄也, 岩城 啓好

    Hip Joint   46 ( 2 )   S208 - S210   2020.08( ISSN:0389-3634

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    2015年5月〜2019年4月に初回片側人工股関節全置換術を施行し術後1週間でT杖歩行または独歩獲得となった159例(男22例、女137例、平均年齢64.42±7.35歳)を、術後1週間で独歩獲得できた独歩群34例と独歩獲得できなかったT杖群125例に分類し、2群間で術前股関節可動域、術側下肢筋力、術側片脚立位時間を比較した。股関節屈曲・外旋・内旋可動域、股関節伸展・外転・内転・外旋・内旋筋力、片足立位時間で有意差を認めた。これを独立変数として投入したロジスティック回帰分析の結果、術後1週間で独歩獲得の可否を予測する因子として、股関節外旋筋が抽出された。独歩獲得に対する股関節外旋筋力向上の臨床的有用性が示唆された。

  • Improvement of a miRNA inhibitor by intracellular selection

    Tachibana A.

    Bioscience, Biotechnology and Biochemistry   84 ( 7 )   1451 - 1454   2020.07( ISSN:09168451

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  • 細胞内選択によるmiRNA阻害剤の改良(Improvement of a miRNA inhibitor by intracellular selection)

    Tachibana Akira, Yamamoto Aiko

    Bioscience, Biotechnology, and Biochemistry   84 ( 7 )   1451 - 1454   2020.07( ISSN:0916-8451

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    強力なmiRNA阻害剤はアルゴノート2(Ago2)上でmiRNAと強く結合する必要があると推測し、その様な阻害剤を細胞内で選択することを試みた。Δ型オリゴ配列とd-21ライブラリーから作成したLidNAライブラリーを標的のmiR-21とともにHEK293T細胞に導入して培養し、その細胞融解物から抗Ago2抗体でLidNA+Ago2複合体を分離・選択した。このサイクルを4回行い、選択したLidNAのmiR-21阻害活性を比較した。その結果、強力な阻害剤は細胞内でゆっくりと分解するが、弱い活性を持つ阻害剤は迅速に細胞内で分解することが判明した。miRNA結合領域周囲の選択された配列を組み合わせると、miRNA阻害活性を促進させることが出来ると考えられた。

  • Structural improvement of LidNA: delta-type LidNA is a potent miRNA inhibitor constructed with unmodified DNA

    Tachibana A.

    Bioscience, Biotechnology and Biochemistry   84 ( 6 )   1168 - 1175   2020.06( ISSN:09168451

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  • Generation of the Rat Monoclonal Antibody against the Extracellular Domain of Human CD63 by DNA Immunization

    Xu L.

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   39 ( 3 )   74 - 76   2020.06

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  • LidNAの構造的改良 デルタ型LidNAは非修飾DNAで作成した強力なmiRNA阻害剤である(Structural improvement of LidNA: delta-type LidNA is a potent miRNA inhibitor constructed with unmodified DNA)

    Tachibana Akira, Komeda Yoshiki, Yamamoto Aiko

    Bioscience, Biotechnology, and Biochemistry   84 ( 6 )   1168 - 1175   2020.06( ISSN:0916-8451

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    miRNAの機能を阻害するDNA(LidNA)を作成するため、非修飾DNAを利用し、長い二本鎖領域の代わりに二本鎖領域を2つ持ったLidNAを作成し、検討した。その結果、LidNAは2'-O-メチル化RNAや架橋型人工核酸よりも強くmiRNA活性を阻害した。LidNAを改良するために、2つの二本鎖領域を結合して、Δ様の形になるような分子を作り、デルタ型LidNAと命名した。内因性と外因性のmiRNAを標的としたデルタ型LidNAを作成したところ、少なくとも10日間強力なmiRNA阻害効果を示した。miR-21を標的としたデルタ型LidNA-21は癌細胞の増殖を阻害した。この新しく開発したLidNAは多くの領域のmiRNA研究にも役立つと考えられた。

  • LidNA, a miRNA inhibitor constructed with unmodified DNA, requires an xxxA insertion sequence in miRNA binding site for its potent inhibitory activity

    Tachibana A.

    FEBS Letters   594 ( 10 )   1608 - 1614   2020.05( ISSN:00145793

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  • 実験室で作成できるキチンシートから成る細胞低接着性表面による均一なスフェロイドの作成(Uniform spheroid formation on a laboratory-made, low cell attachment surface consisting of a chitin sheet)

    Inubushi Yusaku, Tachibana Akira

    Bioscience, Biotechnology, and Biochemistry   84 ( 5 )   997 - 1000   2020.05( ISSN:0916-8451

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    スフェロイドを得るための、実験室で簡便に作れる培養皿の作成を試みた。キトサンを無水酢酸と反応させてキチンゲルを作成した。このゲルを乾燥させ、キチンシートを作成した。HEK293T細胞をキチンシートで培養したところ、スフェロイドが形成された。この細胞は丸い形をしており、キチンシートには付着していなかった。しかし、タイムラプスカメラで長時間撮影したところ、視野範囲に留まっており、キチンシートに弱く付着していると推定された。細胞が付着しない市販のPrimeSurfaceで培養したところ、スフェロイドは急速に移動し、スフェロイドのサイズもばらばらで、細胞の形もゆがんでいた。キチンシートを用いて、ゼラチン培地で培養またはキチンシートに付着しないL929細胞と共培養したところ、均一なスフェロイドが形成された。

  • Improved cancer inhibition by miR-143 with a longer passenger strand than natural miR-143

    Ida H.

    Biochemical and Biophysical Research Communications   524 ( 4 )   810 - 815   2020.04( ISSN:0006291X

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  • Fluorescein isothiocyanate and blue light irradiation alter cell-adhesiveness of cross-linked albumin films for cell patterning Reviewed

    Akira Tachibana, Atsuko Iida, Toshizumi Tanabe

    Bioscience, Biotechnology, and Biochemistry   84 ( 4 )   800 - 803   2020.04

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • フルオレセインイソチオシナートおよび青色光照射は細胞パターニングのための架橋結合アルブミンフィルムの細胞接着性を変化させる(Fluorescein isothiocyanate and blue light irradiation alter cell-adhesiveness of cross-linked albumin films for cell patterning)

    Tachibana Akira, Iida Atsuko, Tanabe Toshizumi

    Bioscience, Biotechnology, and Biochemistry   84 ( 4 )   800 - 803   2020.04( ISSN:0916-8451

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    フルオレセインイソチオシナート(FITC)で処理し、青色光照射し、架橋アルブミン層を親水性にして細胞パターニングする方法を開発した。架橋アルブミンコート皿を種々の濃度のFITCで処理し、青色光照射してL929細胞を培養したところ、高濃度のFITC処理域で細胞接着が観察された。低濃度(0.2mg/mL)のFITCで処理し青色光照射したところ、L929細胞は接着しなかったが、HepG2細胞は接着した。このシステムにより、細胞パターニングを解析することができると考えられた。

  • 幼若期被ばくマウスの脾臓細胞に検出された遺伝子突然変異に短期間カロリー制限がもたらす効果(The effects of short-term calorie restriction on mutations in the spleen cells of infant-irradiated mice)

    Kakomi Saori, Nakayama Takafumi, Yi Shang, Tsuruoka Chizuru, Sunaoshi Masaaki, Morioka Takamitsu, Shimada Yoshiya, Kakinuma Shizuko, Tachibana Akira

    Journal of Radiation Research   61 ( 2 )   187 - 196   2020.03( ISSN:0449-3060

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    幼若期にX線被ばくを受けたマウス脾臓細胞への放射線誘発変異に対し、成熟期でのカロリー制限(CR)でも効果が得られるか実験した。雄性B6C3F1 gpt deltaマウスを用いて、X線被ばく群には1週齢期に3.8GyのX線照射を与え、4週間で離乳後、7週目までは標準食が与えられた。7週目からは無作為に(i)非照射で非CR餌(95kcal/週)が与えられた群、(ii)非照射でCR餌(65kcal/週)が与えられた群、(iii)3.8Gy照射を受け非CR餌(95kcal/週)が与えられた群、(iv)3.8Gy照射を受けCR餌(95kcal/週)が与えられた群の4群に分け実施した。CR開始前の7週齢、8週齢、100日齢に屠殺したマウスから脾臓、肝臓、肺、胸腺を摘出し、凍結保存後、gptアッセイにより点突然変異を検出した。その結果、脾臓由来DNAからgpt遺伝子の点突然変異が検出され、CR群の変異頻度は非CR群に比べ低下が認められ、配列解析からG:C→T:Aへの変異が抑制されていることが明らかにされた。以上の実験結果から、成熟期から開始したCRでも、酸化ストレスを低減されることにより、X線被ばくマウスの遺伝子変異頻度の低減が得られることが立証された。

  • Chitin degradation enzyme-responsive system for controlled release of fibroblast growth factor-2. Reviewed

    Tachibana A, Yasuma D, Takahashi R, Tanabe T

    Journal of Bioscience and Bioengineering   129 ( 1 )   116 - 120   2020.01

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Chitin degradation enzyme-responsive system for controlled release of fibroblast growth factor-2

    Tachibana A.

    Journal of Bioscience and Bioengineering   129 ( 1 )   116 - 120   2020.01( ISSN:13891723

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  • Uniform spheroid formation on a laboratory-made, low cell attachment surface consisting of a chitin sheet Reviewed

    Inubushi Yusaku, Tachibana Akira

    Bioscience, Biotechnology, and Biochemistry   2020

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Investigation of OECD 301F ready biodegradability test to evaluate chemical fate in a realistic environment Reviewed

    Saki Takekoshi, Kotaro Takano, Yoshihide Matoba, Masayuki Sato, Akira Tachibana

    Journal of Pesticide Science   2020

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Generation of the Rat Monoclonal Antibody Against the Extracellular Domain of Human CD63 by DNA immunization Reviewed International coauthorship

    Liu Xu,Kan-ichiro Ihara,Saori Yoshimura,Daijiro Konno, Akira Tachibana, Takeshi Nakanishi, Taro Tachibana

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   2020

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Improvement of a miRNA inhibitor by intracellular selection Reviewed

    Akira Tachibana, Aiko Yamamoto

    Bioscience, Biotechnology, and Biochemistry   2020

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Structural improvement of LidNA: Delta-type LidNA is a potent miRNA inhibitor constructed with unmodified DNA Reviewed

    Akira Tachibana, Yoshiki Komeda, Aiko Yamamoto

    Bioscience, Biotechnology, and Biochemistry   2020

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Improved cancer inhibition by miR-143 with a longer passenger strand than natural miR-143 Reviewed

    Hiroyuki Ida, Toshizumi Tanabe, Akira Tachibana

    Biochemical and Biophysical Research Communications   2020

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • LidNA, a miRNA inhibitor constructed with unmodified DNA, requires an xxxA insertion sequence in miRNA binding site for its potent inhibitory activity Reviewed

    Akira Tachibana, Satoshi Saito, Yukiko Fujiyama, Toshizumi Tanabe

    FEBS Letters   2020

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Generation of the Rat Monoclonal Antibody Against the Extracellular Domain of Human CD63 by DNA immunization Reviewed

    Liu Xu, Kan-ichiro Ihara, Saori Yoshimura, Daijiro Konno, Akira Tachibana, Takeshi Nakanishi, Taro Tachibana

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   2020

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    Publishing type:Research paper (scientific journal)  

  • Uniform spheroid formation on a laboratory-made, low cell attachment surface consisting of a chitin sheet Reviewed

    Inubushi Yusaku, Tachibana Akira

    Bioscience, Biotechnology, and Biochemistry   2020

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    Publishing type:Research paper (scientific journal)  

  • Structural improvement of LidNA: Delta-type LidNA is a potent miRNA inhibitor constructed with unmodified DNA Reviewed

    Akira Tachibana, Yoshiki Komeda, Aiko Yamamoto

    Bioscience, Biotechnology, and Biochemistry   2020

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    Publishing type:Research paper (scientific journal)  

  • LidNA, a miRNA inhibitor constructed with unmodified DNA, requires an xxxA insertion sequence in miRNA binding site for its potent inhibitory activity Reviewed

    Akira Tachibana, Satoshi Saito, Yukiko Fujiyama, Toshizumi Tanabe

    FEBS Letters   2020

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    Publishing type:Research paper (scientific journal)  

  • Improvement of a miRNA inhibitor by intracellular selection Reviewed

    Akira Tachibana, Aiko Yamamoto

    Bioscience, Biotechnology, and Biochemistry   2020

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    Publishing type:Research paper (scientific journal)  

  • Improved cancer inhibition by miR-143 with a longer passenger strand than natural miR-143 Reviewed

    Hiroyuki Ida, Toshizumi Tanabe, Akira Tachibana

    Biochemical and Biophysical Research Communications   2020

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    Publishing type:Research paper (scientific journal)  

  • Fluorescein isothiocyanate and blue light irradiation alter cell-adhesiveness of cross-linked albumin films for cell patterning Reviewed

    Akira Tachibana, Atsuko Iida, Toshizumi Tanabe

    Bioscience, Biotechnology, and Biochemistry   2019

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    Publishing type:Research paper (scientific journal)  

  • Chitin degradation enzyme-responsive system for controlled release of fibroblast growth factor-2. Reviewed

    Tachibana A, Yasuma D, Takahashi R, Tanabe T

    Journal of Bioscience and Bioengineering   2019

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    Publishing type:Research paper (scientific journal)  

  • Acquisition of cell-adhesion capability of the surface of crosslinked albumin films irradiated with atmospheric-pressure plasma jets Reviewed

    Shirafuji Tatsuru, Iwamura Mami, Taga Ryosuke, Kashiwagi Yukiyasu, Nakajima Kota, Ogata Yuji, Tanaka Kenji, Tachibana Akira, Tanabe Toshizumi

    JAPANESE JOURNAL OF APPLIED PHYSICS   55 ( 7 )   2016.07( ISSN:0021-4922

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    Crosslinked albumin films, to which L929 cells do not attach by nature, acquire the L929-cell-adhesion capability by irradiation of an atmospheric-pressure plasma jet (APPJ) of He gas. The number of attached cells was 2.6 × 10<sup>4</sup>cells/cm<sup>2</sup>after the APPJ irradiation for 180 s, while conventional UV photolithography, which was performed in our previous work, required 2 h to obtain the same order of magnitude of the number of attached cells. The contact angle of samples decreased steeply from 105 to 38° in the first 10 s irradiation, but decreased quite gradually from 38 to 32° with increasing irradiation time from 10 to 180 s. In contrast to the nonlinear variation in the contact angles, the number of attached cells almost linearly increased from 4.5 × 10<sup>3</sup>to 2.6 × 10<sup>4</sup>cells/cm<sup>2</sup>with increasing treatment time. X-ray photoelectron spectroscopy of the samples indicated that hydrophilic functional groups of C–C=O gradually formed with increasing APPJ irradiation time up to 180 s. These results suggest that the cell-adhesion capability of the crosslinked albumin films is not simply explained by the decrease in contact angle but also by the formation of oxidized functional groups on the surface. The effects of UV and vacuum UV light from APPJ were negligible, which indicates that the formation of oxidized functional groups on the surface is not caused by photon-assisted surface reactions but by reactions involving chemically active species supplied from APPJ.

    DOI: 10.7567/JJAP.55.07LG03

    CiNii Article

  • Changes in Cell Adhesiveness and Physicochemical Properties of Cross-Linked Albumin Films after Ultraviolet Irradiation Reviewed

    Yamazoe Hironori, Nakanishi Hisashi, Kashiwagi Yukiyasu, Nakamoto Masami, Tachibana Akira, Hagihara Yoshihisa, Tanabe Toshizumi

    LANGMUIR アメリカ化学会   32 ( 1 )   203 - 210   2016.01( ISSN:0743-7463

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    DOI: 10.1021/acs.langmuir.5b03958

  • Changes in Cell Adhesiveness and Physicochemical Properties of Cross-Linked Albumin Films after Ultraviolet Irradiation Reviewed

    Hironori Yamazoe, Hisashi Nakanishi, Yukiyasu Kashiwagi, Masami Nakamoto, Akira Tachibana, Yoshihisa Hagihara, Toshizumi Tanabe

    Langmuir   32 ( 1 )   203 - 210   2016.01( ISSN:1520-5827

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    We discovered the unique cell adhesive properties of ultraviolet (UV)-irradiated albumin films. Albumin films prepared using a cross-linking reagent with epoxy groups maintained native albumin properties, such as resistance to cell adhesion. Interestingly, the cell adhesive properties of films varied depending upon the UV irradiation time
    specifically, cell adhesiveness increased until 2 h of UV irradiation, when the cell number attached to the film was similar to that of culture dishes, and then cell adhesiveness decreased until 20 h of UV irradiation, after which the surface returned to the initial non-adhesive state. To elucidate the molecular mechanisms underlying this phenomenon, we examined the effect of UV irradiation on albumin film properties. The following changes occurred in response to UV irradiation: decreased α-helical structure, cleavage of albumin peptide bonds, and increased hydrophilicity and oxygen content of the albumin film surface. In addition, we found a positive correlation between the degree of cell adhesion and the amount of fibronectin adsorbed on the film. Taken together, UV-induced changes in films highly affect the amount of cell adhesion proteins adsorbed on the films depending upon the irradiation time, which determines cell adhesion behavior.

    DOI: 10.1021/acs.langmuir.5b03958

    PubMed

  • Preparation of keratin and chemically modified keratin hydrogels and their evaluation as cell substrate with drug releasing ability Reviewed

    Nakata Ryo, Osumi Yu, Miyagawa Shoko, Tachibana Akira, Tanabe Toshizumi

    Journal of bioscience and bioengineering   120 ( 1 )   111 - 116   2015.07( ISSN:1389-1723

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    Keratin was extracted as a reduced form from wool, which was then subjected to acetamidation, carboxymethylation or aminoethylation at abundant free cysteine residues to give acetamidated keratin (AAK), carboxymethylated keratin (CMK) and aminoethylated keratin (AEK). Hydrogels were prepared from intact and three chemically modified keratins simply by concentrating their aqueous solution and subsequent cooling. The lowest concentration to form a hydrogel without fluidity was 110 mg/ml for AAK, 120 mg/ml for AEK, 130 mg/ml for keratin and 180 mg/ml for CMK. Comparing with a hydrogel just prepared (swelling ratio: 600-700), each hydrogel slightly shrank in an acidic solution. While AAK hydrogel little swelled in neutral and basic solutions, other hydrogels became swollen and CMK hydrogel reached to dissolution. Hydrogels of keratin, AAK and AEK were found to support cell proliferation, although cell elongation on AAK and AEK hydrogel was a little suppressed. On the other hand, CMK hydrogel did not seem to be suitable for a cell substrate because of its high swelling in culture medium. Evaluation of the hydrogels as a drug carrier showed that keratin and AAK hydrogels were good sustained drug release carriers, which showed the drug release for more than three days, while the release from AEK and CMK hydrogels completed within one day. Thus, keratin and chemically modified keratin hydrogels, especially keratin and AAK hydrogels, were promising biomaterials as a cell substrate and a sustained drug release carrier.

    CiNii Article

  • Preparation of keratin and chemically modified keratin hydrogels and their evaluation as cell substrate with drug releasing ability Reviewed

    Ryo Nakata, Yu Osumi, Shoko Miyagawa, Akira Tachibana, Toshizumi Tanabe

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   120 ( 1 )   111 - 116   2015.07( ISSN:1389-1723

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    Publishing type:Research paper (scientific journal)  

    Keratin was extracted as a reduced form from wool, which was then subjected to acetamidation, carboxymethylation or aminoethylation at abundant free cysteine residues to give acetamidated keratin (AAK), carboxymethylated keratin (CMK) and aminoethylated keratin (AEK). Hydrogels were prepared from intact and three chemically modified keratins simply by concentrating their aqueous solution and subsequent cooling. The lowest concentration to form a hydrogel without fluidity was 110 mg/ml for AAK, 120 mg/ml for AEK, 130 mg/ml for keratin and 180 mg/ml for CMK. Comparing with a hydrogel just prepared (swelling ratio: 600-700), each hydrogel slightly shrank in an acidic solution. While AAK hydrogel little swelled in neutral and basic solutions, other hydrogels became swollen and CMK hydrogel reached to dissolution. Hydrogels of keratin, AAK and AEK were found to support cell proliferation, although cell elongation on AAK and AEK hydrogel was a little suppressed. On the other hand, CMK hydrogel did not seem to be suitable for a cell substrate because of its high swelling in culture medium. Evaluation of the hydrogels as a drug carrier showed that keratin and AAK hydrogels were good sustained drug release carriers, which showed the drug release for more than three days, while the release from AEK and CMK hydrogels completed within one day. Thus, keratin and chemically modified keratin hydrogels, especially keratin and AAK hydrogels, were promising biomaterials as a cell substrate and a sustained drug release carrier. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.

    DOI: 10.1016/j.jbiosc.2014.12.005

  • ケラチンと化学的に修飾したケラチンのヒドロゲルの調製と、薬物徐放能をもった細胞基質としての評価(Preparation of keratin and chemically modified keratin hydrogels and their evaluation as cell substrate with drug releasing ability)

    Nakata Ryo, Osumi Yu, Miyagawa Shoko, Tachibana Akira, Tanabe Toshizumi

    Journal of Bioscience and Bioengineering   120 ( 1 )   111 - 116   2015.07( ISSN:1389-1723

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    羊毛から還元してケラチン(K)を抽出し、アセトアミド化ケラチン(AAK)、カルボキシメチル化ケラチン(CMK)、アミノエチル化ケラチン(AEK)を調製した。その水溶液を濃縮・冷却してヒドロゲルを作成した。調製直後のヒドロゲルと比較して、酸性溶液ではそれぞれのヒドロゲルはわずかに収縮した。一方、中性とアルカリ性溶液では、AAKヒドロゲルはわずかしか膨潤しなかったが、他のヒドロゲルは膨潤し、CMKヒドロゲルは溶解した。K、AAKとAEKのヒドロゲルは細胞増殖をサポートしたが、AAKとAEKのヒドロゲル上の細胞の伸長はわずかに抑制された。CMKヒドロゲルは細胞基質としては不適切であった。薬物の担体として評価したところ、KとAAKのヒドロゲルは3日間薬物を放出し続け、良好な薬物徐放担体であったが、AEKとCMKのヒドロゲルは1日以内で放出してしまうため、不適切であった。

  • Binding affinity of ssDNA is improved by attachment of dsDNA regions Reviewed

    Ida Hiroyuki, Tachibana Akira, Tanabe Toshizumi

    Journal of bioscience and bioengineering   118 ( 3 )   239 - 241   2014.09( ISSN:1389-1723

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    LidNA, a microRNA inhibitor consisting of a microRNA binding ssDNA region sandwiched between dsDNA regions had higher affinity to target oligonucleotides than that without dsDNA region. This enhancement in affinity was found to be owing to the suppressed mobility of ssDNA region by the presence of dsDNA regions.

    CiNii Article

  • ssDNAの結合親和性はdsDNA領域の付加によって促進される(Binding affinity of ssDNA is improved by attachment of dsDNA regions)

    Ida Hiroyuki, Tachibana Akira, Tanabe Toshizumi

    Journal of Bioscience and Bioengineering   118 ( 3 )   239 - 241   2014.09( ISSN:1389-1723

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    腫瘍では多くのmicroRNA(miRNA)が過剰発現しており、miRNA阻害剤であるmiRNAと相補的なアンチセンスオリゴヌクレオチドは、腫瘍の候補治療薬として期待される。以前、新規miRNA阻害剤として、miRNAに相補的な配列を持ち、その両端にdsDNAを付加した未修飾のDNAからなるLidNAを開発した。表面プラズモン共鳴アッセイを用いてmiRNAと相補的なオリゴヌクレオチドの結合能に対し、dsDNAが与える影響を調べたところ、dsDNA領域の付加によ16DNAに対する未修飾のオリゴヌクレオチドの結合能は著明に向上した。今回、そのメカニズムについて、miRNAの末端塩基と隣接する2本鎖領域の末端塩基間のスタッキングと、2本鎖領域の付加によるmiRNA結合領域の構造の安定化の2つの可能性を基に、表面プラズモン共鳴アッセイと蛍光偏光法にて検討した。その結果、結合親和性の亢進は、塩基スタッキングよりもmiRNA結合領域の移動性を抑制したことによるものであった。

  • Preparation of keratin hydrogel/hydroxyapatite composite and its evaluation as a controlled drug release carrier Reviewed

    Nakata Ryo, Tachibana Akira, Tanabe Toshizumi

    MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS   41   59 - 64   2014.08( ISSN:0928-4931

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1016/j.msec.2014.04.016

  • Preparation of keratin hydrogel/hydroxyapatite composite and its evaluation as a controlled drug release carrier Reviewed

    Ryo Nakata, Akira Tachibana, Toshizumi Tanabe

    Materials Science and Engineering C   41   59 - 64   2014.08( ISSN:0928-4931

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    Infection after artificial joint replacement is a serious problem, which requires the re-implantation of prosthesis. To aim at developing bone filling materials having both osteoconductivity and ability as a sustained drug release carrier, composites of wool keratin or carboxymethylated (CM) keratin hydrogels with hydroxyapatite were prepared and evaluated as a sustained drug release carrier. CM-keratin was prepared by the reaction of keratin extracted from wool with iodoacetic acid. Hydrogels were obtained by dropping keratin or CM-keratin solution into CaCl2 solution. The composites were obtained by immersing hydrogels in simulated body fluid (SBF). The introduction of carboxymethyl groups to keratin facilitated the deposition of hydroxyapatite on hydrogel. After 7 days of immersion in SBF, a 4-5 times higher amount of hydroxyapatite was accumulated on CM-keratin hydrogel than that on keratin hydrogel. When salicylic acid was loaded on keratin and CM-keratin hydrogels, a good sustained release was observed
    that is, 90% of a drug was released up to 14 days after 60 and 45% of the initial burst in 1 day. On the other hand, initial release within 1 day was suppressed by forming a composite with hydroxyapatite and the release was almost ceased at 3 days when 60% of the drug was released. Although further improvement to prolong drug release might be necessary, CaKHA and CaCMKHA are expected to be a promising novel type of bone filling materials which has both osteoconductivity and sustained drug release ability. © 2014 Elsevier B.V.

    DOI: 10.1016/j.msec.2014.04.016

    PubMed

  • Long DNA passenger strand highly improves the activity of RNA/DNA hybrid siRNAs Reviewed

    Ida Hiroyuki, Fukuda Kosuke, Tachibana Akira, Tanabe Toshizumi

    Journal of bioscience and bioengineering   117 ( 4 )   401 - 406   2014.04( ISSN:1389-1723

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    Small interfering RNAs (siRNAs) are potent tools in biomedical research, which can reduce the expression level of target proteins through RNAi pathway. They are composed of 19-25 by double strand RNA (dsRNAs), therefore, stimulate dsRNAs dependent interferon responses in a non-specific manner. This problem has prevented siRNAs from being applied as new therapeautic agents. In the present paper, we tried to circumvent interferon responses using RNA/DNA hetero siRNAs (HsiRNAs) composed of RNA guide and DNA passenger strands. It was previously reported that siRNAs which were partially substituted with DNA had RNAi activity and that DNA substitution often caused the activity loss. In our results, HsiRNAs, in which the passenger strand of siRNAs were exchanged with DNA also showed much lower activity than that of parental siRNAs. Here, we found that attachment of 5' flanking sequence to DNA passenger strand improved the activity of HsiRNAs. Furthermore, the effective HsiRNAs induced much lower interferon responses than parental siRNAs. Thus, HsiRNAs with 5' flanking sequence are expected to be novel siRNA drug candidates.

    CiNii Article

  • DNAパッセンジャー鎖の延長はRNA/DNAハイブリッドsiRNAの活性を高める(Long DNA passenger strand highly improves the activity of RNA/DNA hybrid siRNAs)

    Ida Hiroyuki, Fukuda Kosuke, Tachibana Akira, Tanabe Toshizumi

    Journal of Bioscience and Bioengineering   117 ( 4 )   401 - 406   2014.04( ISSN:1389-1723

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    RNAガイド鎖とDNAパッセンジャー鎖から成るRNA/DNAヘテロsiRNA(HsiRNA)を用いてインターフェロン応答性を回避することを試みた。部分的にDNAに置換させたsiRNAは、DNA置換によってRNA干渉(RNAi)活性の消失が多く生じることが報告されていたが、siRNAのパッセンジャー鎖をDNAに置換したHsiRNAは、siRNAよりもさらに活性が低下したにもかかわらず、5'フランキング配列を付加してDNAパッセンジャー鎖の長さを延長するとHsiRNAの活性が高くなることが明らかになった。また、このHsiRNAは、インターフェロン応答性もsiRNAより低く抑えることができた。この5'フランキング配列を付加したHsiRNAは、新規siRNA医薬品の候補物質になりうると考えられた。

  • Binding affinity of ssDNA is improved by attachment of dsDNA regions Reviewed

    Hiroyuki Ida, Akira Tachibana, Toshizumi Tanabe

    Journal of Bioscience and Bioengineering   118 ( 3 )   239 - 241   2014( ISSN:1347-4421

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    Publishing type:Research paper (scientific journal)  

    LidNA, a microRNA inhibitor consisting of a microRNA binding ssDNA region sandwiched between dsDNA regions had higher affinity to target oligonucleotides than that without dsDNA region. This enhancement in affinity was found to be owing to the suppressed mobility of ssDNA region by the presence of dsDNA regions. © 2014 The Society for Biotechnology, Japan.

    DOI: 10.1016/j.jbiosc.2014.02.023

    PubMed

  • Long DNA passenger strand highly improves the activity of RNA/DNA hybrid siRNAs Reviewed

    Hiroyuki Ida, Kosuke Fukuda, Akira Tachibana, Toshizumi Tanabe

    Journal of Bioscience and Bioengineering   117 ( 4 )   401 - 406   2014( ISSN:1347-4421

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    Publishing type:Research paper (scientific journal)  

    Small interfering RNAs (siRNAs) are potent tools in biomedical research, which can reduce the expression level of target proteins through RNAi pathway. They are composed of 19-25 bp double strand RNA (dsRNAs), therefore, stimulate dsRNAs dependent interferon responses in a non-specific manner. This problem has prevented siRNAs from being applied as new therapeautic agents. In the present paper, we tried to circumvent interferon responses using RNA/DNA hetero siRNAs (HsiRNAs) composed of RNA guide and DNA passenger strands. It was previously reported that siRNAs which were partially substituted with DNA had RNAi activity and that DNA substitution often caused the activity loss. In our results, HsiRNAs, in which the passenger strand of siRNAs were exchanged with DNA also showed much lower activity than that of parental siRNAs. Here, we found that attachment of 5' flanking sequence to DNA passenger strand improved the activity of HsiRNAs. Furthermore, the effective HsiRNAs induced much lower interferon responses than parental siRNAs. Thus, HsiRNAs with 5' flanking sequence are expected to be novel siRNA drug candidates. © 2013 The Society for Biotechnology, Japan.

    DOI: 10.1016/j.jbiosc.2013.09.012

    PubMed

  • LidNA, a novel miRNA inhibitor constructed with unmodified DNA Reviewed

    Tachibana Akira, Yamada Yui, Ida Hiroyuki, Saito Satoshi, Tanabe Toshizumi

    FEBS LETTERS (欧州バイオサイエンス学会)   586 ( 10 )   1529 - 1532   2012.05( ISSN:1873-3468

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1016/j.febslet.2012.04.013

  • LidNA, a novel miRNA inhibitor constructed with unmodified DNA Reviewed

    Akira Tachibana, Yui Yamada, Hiroyuki Ida, Satoshi Saito, Toshizumi Tanabe

    FEBS Letters   586 ( 10 )   1529 - 1532   2012.05( ISSN:0014-5793

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    Many miRNA inhibitors have been developed and they are chemically modified oligonucleotides such as 2′-O-methylated RNA and locked nucleic acid (LNA). Unmodified DNA was not yet reported as a miRNA inhibitor because of the low affinity of DNA/miRNA compared to mRNA/miRNA. We designed a structured unmodified DNA that significantly inhibits miRNA function. The clue structure for activity is the miRNA binding site between double stranded regions which is responsible for the miRNA inhibitory activity and tight binding to miRNA. We developed the miRNA inhibitor constructed with unmodified DNA, and named it LidNA, DNA that puts a lid on miRNA function. © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.febslet.2012.04.013

    PubMed

  • Control of antibiotic delivery from TCP along with HA on bone cement in the interface bioactive bone cement for prevention of infection in joint replacement Reviewed

    Mizokawa S, Arita T, Tachibana A, Tanabe T, Oonishi H Jr., Oonish H

    Key Engineering Materials   493-494   186 - 190   2012

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  • Antibiotic Delivery Effects from HA on Bone Cement in the Interface Bioactive Bone Cement for Prevention of Infection in Joint Replacement Reviewed

    Oonishi H Jr, Arita T, Tachibana A, Tanabe T, Mizokawa S, Oonish H

    Key Engineering Materials   493-494   361 - 365   2012

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    Publishing type:Research paper (scientific journal)   Kind of work:Single Work  

  • Antibiotic delivery effects from HA on bone cement in the interface bioactive bone cement for prevention of infection in joint replacement Reviewed

    Hiroyuki Oonishi Jr., Tomonori Arita, Akira Tachibana, Toshizumi Tanabe, Shigekazu Mizokawa, Hironobu Oonishi

    Key Engineering Materials   493-494   361 - 365   2012( ISSN:1013-9826

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    Publishing type:Research paper (international conference proceedings)  

    Antibiotics release impregnated in HA granules, which were used in IBBC to prevent infection after total joint arthroplasty, was measured. For antibiotics, Flumarin, Vancomycin, Pansporin and Firstcin were used. Two models of antibiotics release were assumed
    Model [I]: antibiotics release from surroundings of HA granules immediately after surgery and Model [II]: antibiotics release loaded on HA after antibiotics release from surroundings of HA granules as follows
    (1) loading in normal pressure and (2) loading in reduced pressure. The amount of antibiotics loaded on HA is higher when loading is conducted under reduced pressure than that under normal pressure. Firstcin showed the highest loaded amount and the most desirable sustained release pattern. The antibiotics release from HA are varid depending on the antibiotics used. © (2012) Trans Tech Publications.

    DOI: 10.4028/www.scientific.net/KEM.493-494.361

  • Control of antibiotic delivery from TCP along with ha on bone cement in the interface bioactive bone cement for prevention of infection in joint replacement Reviewed

    Shigekazu Mizokawa, Tomonori Arita, Akira Tachibana, Toshizumi Tanabe, Hiroyuki Oonishi, Hironobu Oonishi

    Key Engineering Materials   493-494   186 - 190   2012( ISSN:1013-9826

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    Publishing type:Research paper (international conference proceedings)  

    Since 1985, HA granules were interposed on the interface between bone and bone cement at the cementation (Interface Bioactive Bone Cement : IBBC) in THA to prevent generation of connective tissue and osteolysis. To prevent infection, β-TCP impregnated with antibiotics along with HA granules was used. As TCP is resorbable, antibiotic release can be controlled. β-TCP granules were impregnated with antibiotics of folmoxef sodium (F), Vancomycine hydrochloride (V) cefortiam dihydrochloride (C) and cefozopran hydrochloride (CE). Three models of antibiotic release were assumed. Model [1] was antibiotic release from surroundings of β-TCP granules. Model [2] was the condition loaded under normal and reduced pressure. In Model [3], β-TCP was dissolved gradually in EDTA, as the model in the living body. In model [1], the amount of release of F, V and C was 3280, 300 and 3 μg, respectively and completed in 30 hours. In model [2], the amount of release of F, V, C and CE was 16, 8, 0 and 8000 μg in reduced pressure, respectively. The release of F, V and C completed within 24 hours and that of CE was in 6 days. In model [3], released amount of C and CE was 116 and 7100 μg, respectively and completed in 19 days. © (2012) Trans Tech Publications.

    DOI: 10.4028/www.scientific.net/KEM.493-494.186

  • 合成生物学(現代生物学入門 第9巻), 浅島誠・黒岩常祥・小原雄治(編), 柳川弘志・土居信英・板谷光泰・菅原正・四方哲也(著), A5判, 220頁, 3,150円, 岩波書店

    立花亮

    生物工学会誌 : Seibutsu-kogaku Kaishi   88 ( 7 )   361   2010.07

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  • Recombinant human serum albumin hydrogel as a novel drug delivery vehicle Reviewed

    Hirose Masaaki, Tachibana Akira, Tanabe Toshizumi

    MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS   30 ( 5 )   664 - 669   2010.06( ISSN:0928-4931

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1016/j.msec.2010.02.020

  • Recombinant human serum albumin hydrogel as a novel drug delivery vehicle Reviewed

    Masaaki Hirose, Akira Tachibana, Toshizumi Tanabe

    MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS   30 ( 5 )   664 - 669   2010.06( ISSN:0928-4931

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    Publishing type:Research paper (scientific journal)  

    Serum albumin acts as a physiological carrier for various compounds including drugs. A hydrogel consisting of recombinant human serum albumin (rHSA) was prepared to take advantage of drug binding ability of albumin for a sustained drug release carrier. The hydrogel was prepared by mixing rHSA and dithiothreitol and casted to a polystyrene mold. Hydrogel formation was thought to occur through the intermolecular interaction of the hydrophobic groups by protein denaturation. The release of sodium benzoate and salicylic acid from the hydrogel completed in 2 h, while warfarin release continued for 24 h. The total amounts of the drugs released from 100 mg of 15 and 5% rHSA hydrogel were 2.3 and 1.4 mu mol for warfarin, 1.4 and 1.1 mu mol for salicylic acid and 0.9 and 0.9 mu mol for sodium benzoate. These results reflected the order of the binding ability of drugs for intact albumin indicating that the drug binding ability of HSA still remained after the hydrogel formation. However, fibroblast cells attached and proliferated well on the hydrogel, indicating that denaturation of rHSA proceeded to the extent to allow the cell attachment. The present rHSA hydrogel might be suitable for a sustained release carrier of drugs having affinity for albumin. (c) 2010 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.msec.2010.02.020

  • 『iPS細胞の産業的応用技術』, 山中伸弥監修, B5判, 237頁, 定価8,400円, シーエムシー出版

    立花亮

    生物工学会誌 : Seibutsu-kogaku Kaishi   87 ( 11 )   563   2009.11

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  • 学生・研究者のための 使える! PowerPointスライドデザイン 伝わるプレゼン1つの原理と3つの技術, 宮野公樹著, B5判, 144頁, 定価1,890円, 化学同人

    立花亮

    生物工学会誌 : Seibutsu-kogaku Kaishi   87 ( 7 )   362   2009.07

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  • Preparation of long sticky ends for universal ligation-independent cloning : Sequential T4 DNA polymerase treatments Reviewed

    TACHIBANA Akira, TOHIGUCHI Kazuo, UENO Takayuki, SETOGAWA Yuichi, HARADA Ayako, TANABE Toshizumi

    Journal of bioscience and bioengineering   107 ( 6 )   668 - 669   2009.06( ISSN:13891723

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    Ligation-independent cloning (LIC) is a useful method for efficient directional cloning of a PCR product. LIC requires a specially designed vector containing a long stretch of sequence that is missing any one of the four nucleotides. When the linearized vector is treated with T4 DNA polymerase, in the presence of the absent base, long single-stranded overhangs are generated that are suitable for cloning. In this study, long and efficient sticky ends for LIC were produced by sequential T4 DNA polymerase treatments at non-specific sequences on a commercially available vector. All restriction enzyme sites become available in the current LIC.

    CiNii Article

  • Preparation of long sticky ends for universal ligation-independent cloning: Sequential T4 DNA polymerase treatments Reviewed

    Akira Tachibana, Kazuo Tohiguchi, Takayuki Ueno, Yuichi Setogawa, Ayako Harada, Toshizumi Tanabe

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   107 ( 6 )   668 - 669   2009.06( ISSN:1389-1723

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    Publishing type:Research paper (scientific journal)  

    Ligation-independent cloning (LIC) is a useful method for efficient directional cloning of a PCR product. LIC requires a specially designed vector containing a long stretch of sequence that is missing any one of the four nucleotides. When the linearized vector is treated with T4 DNA polymerase, in the presence of the absent base, long single-stranded overhangs are generated that are suitable for cloning. In this study, long and efficient sticky ends for LIC were produced by sequential T4 DNA polymerase treatments at non-specific sequences on a commercially available vector. All restriction enzyme sites become available in the current LIC. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.

    DOI: 10.1016/j.jbiosc.2009.01.019

  • システム生物学入門-生物回路の設計原理-, Uri Alon著, 倉田博之・宮野悟訳, B5判, 308ページ, 5,460円(税込), 共立出版

    立花亮

    生物工学会誌 : Seibutsu-kogaku Kaishi   87 ( 2 )   112   2009.02

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  • Preparation of long sticky ends for universal ligation-independent cloning : Sequential T4 DNA polymerase treatments Reviewed

    立花 亮

    Journal of Bioscience and Bioengineering 107(6)(in press)   2009

  • Fabrication of highly porous keratin sponges by freeze-drying in the presence of calcium alginate beads Reviewed

    Hamasaki Shinichi, Tachibana Akira, Tada Daisuke, Yamauchi Kiyoshi, Tanabe Toshizumi

    MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS   28 ( 8 )   1250 - 1254   2008.12( ISSN:0928-4931

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.msec.2007.11.008

  • Fabrication of highly porous keratin sponges by freeze-drying in the presence of calcium alginate beads Reviewed

    Shinichi Hamasaki, Akira Tachibana, Daisuke Tada, Kiyoshi Yamauchi, Toshizumi Tanabe

    MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS   28 ( 8 )   1250 - 1254   2008.12( ISSN:0928-4931

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    Publishing type:Research paper (scientific journal)  

    Novel fabrication method of highly porous and flexible keratin sponges was developed by combining a particulate-leaching method and a freeze-drying method. Reduced keratin aqueous solution was mixed with dried calcium alginate beads and was lyophilized to give keratin/calcium alginate complex, which was subsequently treated with EDTA solution to leach out calcium alginate beads. The resultant keratin sponge was flexible enough to handle even in dried state because of its quite high porosity (98.9 +/- 0.1%), which was brought about by the large and small pores formed by the elimination of calcium alginate beads and water. The sponge supported the attachment and the proliferation of mouse fibroblast cells. Thus, the keratin sponge given by the present fabrication method afforded one alternative as a cell scaffold for tissue engineering. (C) 2007 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.msec.2007.11.008

  • A film of collagen/calcium phosphate composite prepared by enzymatic mineralization in an aqueous phase Reviewed

    Tomomatsu Ohki, Tachibana Akira, Yamauchi Kiyoshi, Tanabe Toshizumi

    JOURNAL OF THE CERAMIC SOCIETY OF JAPAN   116 ( 1349 )   10 - 13   2008.01( ISSN:1882-0743

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  • A film of collagen/calcium phosphate composite prepared by enzymatic mineralization in an aqueous phase Reviewed

    Ohki Tomomatsu, Akira Tachibana, Kiyoshi Yamauchi, Toshizumi Tanabe

    JOURNAL OF THE CERAMIC SOCIETY OF JAPAN   116 ( 1349 )   10 - 13   2008.01( ISSN:1882-0743

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    Collagen/calcium phosphate composite materials were prepared using an alkaline phosphatase-catalyzed hydrolysis of water-soluble phosphate esters in the presence of calcium ions. We prepared a collagen/calcium phosphate composite film by adding sequentially calcium chloride, disodium glycerophosphate and alkaline phosphatase to collagen solution and subsequent drying of the resultant composite suspension in a template. The calcium phosphate contained in the film was determined to be mainly hydroxyapatite from FT-IR and X-ray diffraction analysis. The film little swelled in water to keep its original morphology, while a collagen film became solubilized. This suggests the binding between collagen and calcium phosphate in complex materials. When the preosteoblast cell line, MC3T3-E1 cells were cultured on the film, the film was found to support the osteoblastic differentiation from the measurement of alkaline phosphatase activity., which is an early differentiation marker.

  • A film of collagen/calcium phosphate composite prepared by enzymatic mineralization in an aqueous phase

    TOMOMATSU Ohki, TACHIBANA Akira, YAMAUCHI Kiyoshi, TANABE Toshizumi

    Journal of the Ceramic Society of Japan   116 ( 1349 )   10 - 13   2008( ISSN:18820743

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    Collagen/calcium phosphate composite materials were prepared using an alkaline phosphatase-catalyzed hydrolysis of water-soluble phosphate esters in the presence of calcium ions. We prepared a collagen/calcium phosphate composite film by adding sequentially calcium chloride, disodium glycerophosphate and alkaline phosphatase to collagen solution and subsequent drying of the resultant composite suspension in a template. The calcium phosphate contained in the film was determined to be mainly hydroxyapatite from FT-IR and X-ray diffraction analysis. The film little swelled in water to keep its original morphology, while a collagen film became solubilized. This suggests the binding between collagen and calcium phosphate in complex materials. When the preosteoblast cell line, MC3T3-E1 cells were cultured on the film, the film was found to support the osteoblastic differentiation from the measurement of alkaline phosphatase activity, which is an early differentiation marker.<br>

    DOI: 10.2109/jcersj2.116.10

    CiNii Article

  • Albumin-crosslinked alginate hydrogels as sustained drug release carrier Reviewed

    Tada Daisuke, Tanabe Toshizumi, Tachibana Akira, Yamauchi Kiyoshi

    MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS   27 ( 4 )   870 - 874   2007.05( ISSN:0928-4931

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    DOI: 10.1016/j.msec.2006.10.008

  • Recognition of four structurally resembled benzoic acid derivatives by albumin-crosslinked poly(acrylamide) hydrogel Reviewed

    Tada Daisuke, Tanabe Toshizumi, Tachibana Akira, Yamuchi Kiyoshi

    MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS   27 ( 4 )   895 - 897   2007.05( ISSN:0928-4931

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    DOI: 10.1016/j.msec.2006.10.009

  • Albumin-crosslinked alginate hydrogels as sustained drug release carrier Reviewed

    Daisuke Tada, Toshizumi Tanabe, Akira Tachibana, Kiyoshi Yamauchi

    MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS   27 ( 4 )   870 - 874   2007.05( ISSN:0928-4931

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    To take advantage of the drug-binding ability of albumin as a component of drug delivery system, we have prepared hydrogels consisting of alginic acid (AL) and recombinant human serum albumin (rHSA) by dehydrating condensation using N-hydroxysuccininimide and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide. As rHSA content increased, the swelling ratio of the hydrogel decreased, indicating rHSA functioned as a crosslinker. In fact, trypsin treatment solubilized the hydrogel. Salicylic acid, which has high affinity for rHSA, was loaded most on the hydrogel of the highest rHSA content despite the lowest swelling ratio. Meanwhile, drugs with less affinity for HSA such as o-anisic acid and benzoic acid were preferably loaded on the hydrogel having the highest swelling ratio but the lowest HSA content. The release of salicylic acid from the hydrogel sustained longer than o-anisic acid and benzoic acid, reflecting the affinity of the drug for HSA. Furthermore, the hydrogel could carry much of positively charged dibucaine by the interaction with anionic alginic acid and showed highly sustained release. Since the safety of AL and rHSA in medical use is guaranteed, rHSA-crosslinked AL hydrogel is expected to use as a sustained drug release carrier for drugs having affinity for HSA and those with cationic charge. (C) 2006 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.msec.2006.10.008

  • Recognition of four structurally resembled benzoic acid derivatives by albumin-crosslinked poly(acrylamide) hydrogel Reviewed

    Daisuke Tada, Toshizumi Tanabe, Akira Tachibana, Kiyoshi Yamuchi

    MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS   27 ( 4 )   895 - 897   2007.05( ISSN:0928-4931

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    Poly(acrylamide) hydrogels crosslinked by bovine serum albumin (BSA) were prepared by the introduction of vinyl groups into BSA and subsequent co-polymerization with acrylamide (AAm). The bydrogels were loaded with four structurally resembled benzoic acid derivatives such as salicylic acid, o-anisic acid, salicylamide and sodium benzoate, and their release from the hydrogel was investigated. The affinity of these four compounds for BSA gradually decreased in a following order; salicylic acid &gt; o-anisic acid &gt; salicylamide &gt; sodium benzoate. The amounts of compounds loaded on a hydrogel of high BSA content and the duration of their release were found to be dependent on the affinity for BSA clearly reflecting the subtle difference in BSA affinity of each compound. Therefore, this hydrogel would be used not only as a sustained drug release carrier, but also a facile tool to evaluate the binding of various compounds to serum albumin. (C) 2006 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.msec.2006.10.009

  • SmaI cloning with regeneration of the SmaI site for sequential PCR product cloning Reviewed

    Tachibana Akira, Tanabe Toshizumi, Yamauchi Kiyoshi

    ANALYTICAL BIOCHEMISTRY   361 ( 1 )   143 - 145   2007.02( ISSN:0003-2697

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    DOI: 10.1016/j.ab.2006.10.004

  • SmaI cloning with regeneration of the SmaI site for sequential PCR product cloning Reviewed

    Akira Tachibana, Toshizumi Tanabe, Kiyoshi Yamauchi

    ANALYTICAL BIOCHEMISTRY   361 ( 1 )   143 - 145   2007.02( ISSN:0003-2697

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    DOI: 10.1016/j.ab.2006.10.004

  • Smal eloning with regeneration of the Smal site for sequential PCR product cloning

    立花 亮

    Analytical Biochemistry 361   143 - 145   2007

  • SmaI cloning with regeneration of the SmaI site for sequential PCR product cloning

    立花 亮

    Analytical Biochemistry 361   143 - 145   2007

  • Modified Keratin Sponge : Binding of Bone Morphogenetic Protein-2 and Osteoblast Differentiation Reviewed

    Tachibana Akira, Nishikawa Yuji, Nishino Masaaki, KANEKO Sumika, TANABE Toshizumi, YAMAUCHI Kiyoshi

    公益社団法人日本生物工学会 Journal of bioscience and bioengineering   102 ( 5 )   425 - 429   2006.11( ISSN:13891723

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    A keratin sponge was chemically modified to obtain carboxyl and amino sponges by the alkylation of a large amount of active SH group on keratin proteins with iodoacetic acid and 2-bromoethylamine, respectively. The carboxyl sponge having a large amount of carboxyl group was a scaffold that could bind significant amounts of basic bioactive proteins, such as lysozyme and bone morphogenetic protein (BMP)-2, and drugs. Lysozyme (maximum 3.7mg), a model of basic cytokines such as BMP-2, was absorbed by the carboxyl sponge (4.8mg), but not by the amino sponge. The lysozyme was rapidly released from the carboxyl sponge in a buffer containing at a concentration higher than 0.5M, but at 0.15M, near the physiological ionic strength, after initial burst (only 11%), no significant release was observed (15%, 48h). BMP-2 also bound the carboxyl sponge. The preosteoblast cells grown inside the BMP-2-loaded sponge differentiated, whereas those grown outside the sponge did not, suggesting that no significant amount of BMP-2 leaked into the surrounding media. The effects of BMP-2 were confined inside modified keratin sponge. Therefore, using in vivo, we expect that only internal osteogenesis will be induced and that no external heterotopis ossification will be induced.

    CiNii Article

  • Modified keratin sponge: Binding of bone morphogenetic protein-2 and osteoblast differentiation Reviewed

    Akira Tachibana, Yuji Nishikawa, Masaaki Nishino, Sumika Kaneko, Toshizumi Tanabe, Kiyoshi Yamauchi

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   102 ( 5 )   425 - 429   2006.11( ISSN:1389-1723

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    Publishing type:Research paper (scientific journal)  

    A keratin sponge was chemically modified to obtain carboxyl and amino sponges by the alkylation of a large amount of active SH group on keratin proteins with iodoacetic acid and 2-bromo-ethylamine, respectively. The carboxyl sponge having a large amount of carboxyl group was a scaffold that could bind significant amounts of basic bioactive proteins, such as lysozyme and bone morphogenetic protein (BMP)-2, and drugs. Lysozyme (maximum 3.7 mg), a model of basic cytokines such as BMP-2, was absorbed by the carboxyl sponge (4.8 mg), but not by the amino sponge. The lysozyme was rapidly released from the carboxyl sponge in a buffer containing at a concentration higher than 0.5 M, but at 0.15 M, near the physiological ionic strength, after initial burst (only 11%), no significant release was observed (15%, 48 h). BMP-2 also bound the carboxyl sponge. The preosteoblast cells grown inside the BMP-2-loaded sponge differentiated, whereas those grown outside the sponge did not, suggesting that no significant amount of BMP-2 leaked into the surrounding media. The effects of BMP-2 were confined inside modified keratin sponge. Therefore, using in vivo, we expect that only internal osteogenesis will be induced and that no external heterotopis ossification will be induced.

    DOI: 10.1263/jbb.102.425

  • Modified keratin sponge : Binding of Bone Morphogenetic Protein-2 and Osteoblast Differentiation

    立花 亮

    J. Biosci. Bioeng. 102・5   425 - 429   2006

  • Drug Release from Hydrogel Containing Albumin as Crosslinker Reviewed

    Tada Daisuke, Tanabe Toshizumi, Tachibana Akira, YAMAUCHI Kiyoshi

    公益社団法人日本生物工学会 Journal of bioscience and bioengineering   100 ( 5 )   551 - 555   2005.11( ISSN:13891723

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    Albumin is the major plasma protein and acts as a physiological carrier for various compounds including drugs. To take advantage of the drug-binding ability of albumin for a drug delivery system, we have prepared hydrogels consisting of acrylamide (AAm) and bovine serum albumin (BSA) by introducing three to four vinyl groups into one BSA molecule and subsequently copolymerizing it with AAm. The resultant hydrogel was solubilized by trypsin treatment, since BSA served as a crosslinker in the hydrogel. The BSA-crosslinked hydrogel (BSA-AAm hydrogel) was loaded with salicylic acid or sodium benzoate and their release was investigated. The BSA-AAm hydrogel released much more salicylic acid than sodium benzoate. In addition, the amount of released salicylic acid increased with the BSA content of the hydrogel, despite a decrease in the swelling ratio of the hydrogel. On the other hand, the amount of released sodium benzoate increased with the swelling ratio. When a hydrogel crosslinked with N, N'-methylenebis (acrylamide) was used as a control, both drugs showed release tendencies similar to that of sodium benzoate from the BSA-AAm hydrogel. Furthermore, the salicylic acid release was sustained longer on the BSA-AAm hydrogel than the sodium benzoate release. Taken together, it is thought that albumin in the BSA-AAm hydrogel preferentially adsorbs salicylic acid and contributes to the high drug loading and the sustained release of salicylic acid.

    CiNii Article

  • Drug release from hydrogel containing albumin as crosslinker Reviewed

    D Tada, T Tanabe, A Tachibana, K Yamauchi

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   100 ( 5 )   551 - 555   2005.11( ISSN:1389-1723

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    Publishing type:Research paper (scientific journal)  

    Albumin is the major plasma protein and acts as a physiological carrier for various compounds including drugs. To take advantage of the drug-binding ability, of albumin for a drug delivery system, we have prepared hydrogels consisting of acrylamide (AAm) and bovine serum albumin (BSA) by introducing three to four vinyl groups into one BSA molecule and subsequently copolymerizing it with AAm. The resultant hydrogel was solubilized by trypsin treatment, since BSA served as a crosslinker in the hydrogel. The BSA-crosslinked hydrogel (BSA-AAm hydrogel) was loaded with salicylic acid or sodium benzoate and their release was investigated. The BSA-AAm hydrogel released much more salicylic acid than sodium benzoate. In addition, the amount of released salicylic acid increased with the BSA content of the hydrogel, despite a decrease in the swelling ratio of the hydrogel. On the other hand, the amount of released sodium benzoate increased with the swelling ratio. When a hydrogel crosslinked with N,N'-methylenebis (acrylamide) was used as a control, both drugs showed release tendencies similar to that of sodium benzoate from the BSA-AAm hydrogel. Furthermore, the salicylic acid release was sustained longer on the BSA-AAm hydrogel than the sodium benzoate release. Taken together, it is thought that albumin in the BSA-AAm hydrogel preferentially adsorbs salicylic acid and contributes to the high drug loading and the sustained release of salicylic acid.

    DOI: 10.1263/hbb.100.551

  • Rapid fabrication of keratin-hydroxyapatite hybrid sponges toward osteoblast cultivation and differentiation Reviewed

    Tachibana A, Kaneko S, Tanabe T, Yamauchi K

    BIOMATERIALS   26 ( 3 )   297 - 302   2005.01( ISSN:0142-9612

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1016/j.biomaterials.2004.02.032

  • Rapid fabrication of keratin-hydroxyapatite hybrid sponges toward osteoblast cultivation and differentiation Reviewed

    A Tachibana, S Kaneko, T Tanabe, K Yamauchi

    BIOMATERIALS   26 ( 3 )   297 - 302   2005.01( ISSN:0142-9612

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    Publishing type:Research paper (scientific journal)  

    Wool keratin sponges were reported to be useful scaffolds for long-term and high-density cell cultivation (J. Biotechnol. 93 (2002) 165). The hybrid of the keratin sponges with calcium phosphate materials gave the additional function. Two rapid fabrication methods for calcium phosphate hybrid biomaterials were described. Firstly, the CaP-precipitated sponges were obtained by only the immersion of the carboxyl-sponges, chemically introduced with high amount of carboxyl groups on the sponges, in calcium and phosphate ions containing buffers such as PBS(+) for only 1-3 days. Neither sponge, introduced with amino or amido groups or non-treated, gave significant calcium phosphate precipitation. The carboxyl-sponges were mimics of matrix gamma-carboxyglutamic acid protein, which is responsible for osteoblast calcification. Secondly, the hydroxyapatite particle suspension was added onto carboxyl-sponges to fabricate trapped sponge. The trapped hydroxyapatite particles might interact with keratin protein of the sponge walls. Preliminary experiments measuring the expression of alkaline phosphatase, early osteoblast differentiation marker, suggested that both hybrid sponges, CaP-precipitated and trapped sponges, alter the differentiation pattern of preosteoblasts, MC3T3-E1. (C) 2004 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.biomaterials.2004.02.032

  • Rapid fabrication of keratin-hydroxyapatite hybrid sponges toward osteoblast cultivation and differentiation

    立花 亮

    Biomaterials 26・3   233 - 348   2005

  • Keratin sponge: immobilization of lysozyme

    Journal of Bioscience and Bioengineering   96 ( 3 )   307 - 309   2003

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    Publishing type:Research paper (scientific journal)  

  • Fabrication of wool keratin sponge scaffolds for long-term cell cultivation Reviewed

    J. Biotech.   93   165 - 170   2001

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Thiolated dermal bovine collagen as a novel support for bioactive substances - conjugation with lysozyme Reviewed

    86   1 - 8   2001

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Preparation and characterization of keratin-chitosan composite film Reviewed

    Biomaterials   22   817 - 825   2001

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Novel prenyltransferase gene encoding farnesylgeranyl diphosphate synthase from a hyperthermophilic archaeon, Aeropyrum pernix. Molecular evolution with alteration in product specificity. Reviewed

    267   321 - 328   2000

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Non-Radiochemical 3-Hydroxy-3-Methylglutaryl-Coenzyme A Synthase Assay by Reversed-Phase HPLC without Using Ion-Pair Reagent Reviewed

    86   523 - 526   1998

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Evidence for farnesol-mediated isoprenoid synthhesis regulation in a halophilic archaeon, Haloferax volcanii Reviewed

    FEBS Lett.   379   43 - 46   1996

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • A novel prenyltransferase, farnesylgeranyl diphosphate synthase, from the haloalkalophilic archaeon, Natronobacterium pharaonis Reviewed

    341   291 - 294   1994

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    Publishing type:Research paper (scientific journal)   Kind of work:Single Work  

  • Potassium-stimulating mechanism of geranylgeranyl diphosphate synthase of Methanobacterium thermofomicicum SF-4 Reviewed

    114   389 - 392   1993

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Some properties of FD2420D2-reducing hydrogenase of Methanobacterium thermoformicicum strain SF-4 Reviewed

    57   156 - 157   1993

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Purification and characterization of geranylgeranyl diphosphate synthase from Methanobacterium thermoformicicum SF-4 Reviewed

    57   1129 - 1133   1993

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Purification and some properties of aspartate aminotransferase of Methanobacterium thermoformicicum SF-4 Reviewed

    54   625 - 631   1990

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Characterization of a thermophilic formate-utilizing methanogen, Methanobacterium thermoformicicum SF-4 Reviewed International coauthorship

    53   533 - 534   1990

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

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Books and Other Publications

  • バイオ実験を安全に正しく行うために

    青柳 秀紀、立花 亮 他( Role: Joint author)

    化学同人、生物工学会  2018.09 

  • バイオ実験を安全に正しく行うために

    青柳 秀紀, 立花 亮 他( Role: Joint author)

    化学同人、生物工学会  2018.09 

MISC

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Presentations

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Outline of collaborative research (seeds)

  • Cell cultivation with protein biomaterials

    現在はいつでも交流可能です

Grant-in-Aid for Scientific Research

  • 骨再生のための時間差マルチ徐放システムの開発

    Grant-in-Aid for Scientific Research(C)  2011.04

  • 再生医療のための分泌物を指標とした細胞検出法の開発

    Grant-in-Aid for Scientific Research(C)  2010.04

Other subsidies, etc.

  • miRNAプロファイルモジュレーションシステムの開発

    未設定  2012.09

Charge of on-campus class subject

  • バイオ工学実験Ⅰ

    2019     Undergraduate

  • 生物化学基礎

    2019     Undergraduate

  • 創薬分子工学特論

    2019     Graduate school

  • バイオ工学実験法

    2019     Undergraduate

  • 化学バイオ工学概論

    2019     Undergraduate

  • 生化学Ⅰ

    2019     Undergraduate

  • 機能分子工学特論

    2014    

  • バイオ工学実験Ⅰ

    2014     Undergraduate

  • 化学バイオ工学概論

    2014     Undergraduate

  • バイオ工学実験法

    2014     Undergraduate

  • 生化学Ⅰ

    2014     Undergraduate

  • 生化学1

    2013     Undergraduate

  • バイオ工学実験法

    2013     Undergraduate

  • 化学バイオ工学概論

    2013     Undergraduate

  • バイオ工学実験Ⅰ

    2013     Undergraduate

  • 機能分子工学特論

    2013    

  • 機能分子工学特論

    2012    

  • バイオ工学実験Ⅰ

    2012     Undergraduate

  • 化学バイオ工学概論

    2012     Undergraduate

  • バイオ工学実験法

    2012     Undergraduate

  • 生化学1

    2012     Undergraduate

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Social Activities

  • 細胞製剤調製や薬剤スクリーニングに好適な細胞スフェロイドの迅速作製法

    Role(s): Lecturer

    2017.08 - 2017.09

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    Audience: Researchesrs, Company

    Type:Seminar, workshop

  • 細胞製剤調製や薬剤スクリーニングに好適な細胞スフェロイドの迅速作製法

    Role(s): Lecturer

    2017.08 - 2017.09

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    Audience: Researchesrs, Company

    Type:Seminar, workshop

  • オープンキャンパス

    Role(s): Guest, Planner

    2017.08

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    Audience: High school students, General public

    Type:University open house

  • オープンキャンパス

    Role(s): Guest, Planner

    2017.08

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    Audience: High school students, General public

    Type:University open house