Updated on 2022/09/08

写真a

 
ICHIKAWA Naoki
 
Organization
Graduate School of Human Life and Ecology Division of Human Life and Ecology Associate Professor
School of Human Life and Ecology Department of Nutrition
Title
Associate Professor
Affiliation
Institute of Human Life and Ecology

Position

  • Graduate School of Human Life and Ecology Division of Human Life and Ecology 

    Associate Professor  2022.04 - Now

  • School of Human Life and Ecology Department of Nutrition 

    Associate Professor  2022.04 - Now

Degree

  • Doctor of Science ( Osaka University ) (   Osaka University )

Research Areas

  • Life Science / Functional biochemistry  / Biochemistry and Molecular Bio

  • Life Science / Metabolism and endocrinology  / Metabolomics

Research Interests

  • ATP synthase

  • 活性調節

  • IF1 protein

  • ATPase inhibitor

  • Saccharomyces cerevisiae

  • mitochondria

  • Biochemistry

Research subject summary

  • ミトコンドリアATP合成酵素の調節機構の研究

Research Career

Professional Memberships

  • The Japanese Biochemical Society

      Domestic

  • Japanese Society of Nutrition and Food Science

      Domestic

  • Japan Society for Bioscience, Biotechnology, and Agrochemistry

      Domestic

  • The Molecular Biology Society of Japan

      Domestic

Awards

  • 永年勤続表彰(大阪市立大学)

    2017.09   大阪市立大学  

Education

  • Osaka University   The second semester of doctoral program   Graduated/Completed

    1988.04 - 1991.03

  • Osaka University   The first semester of doctoral program   Graduated/Completed

    1986.04 - 1988.03

Papers

  • The region from phenylalanine-28 to lysine-50 of a yeast mitochondrial ATPase inhibitor (IF1) forms an α-helix in solution Reviewed

    Li Sun, Naomi Nakamae, and Naoki Ichikawa

    J. Bioenerg. Biomembr.   47 ( 6 )   457 - 465   2015

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1007/s10863-015-9627-7

  • Characterization of unstable pEntYN10 from enterotoxigenic Escherichia coli (ETEC) O169:H41 Reviewed

    Erika Ban, Yuka Yoshida, Mitsuko Wakushima, Takeaki Wajima, Takashi Hamabata, Naoki Ichikawa et al.

    Virulence   6 ( 8 )   735 - 744   2015( ISSN:ISSN: 2150-5594

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Glutamic acid in the inhibitory site of mitochondrial ATPase inhibitor, IF1, participates in pH sensing in both mammals and yeast Reviewed

    Chiyoko Ando and Naoki Ichikawa

    J. Biochem.   144 ( 4 )   547 - 553   2008

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1093/jb/mvn100

  • Caenorhabditis elegans MAI-1 protein, which is similar to mitochondrial ATPase inhibitor (IF1), can inhibit yeast F0F1-ATPase but cannot be transported to yeast mitochondria Reviewed

    J. Bioenerg. Biomembr.   38   93 - 99   2006( ISSN:0145-479X

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1007/s10863-006-9009-2

  • Mitochondrial ATP synthase residue βArginine-408, which interacts with the inhibitory site of regulatory protein IF1, is essential for the function of the enzyme Reviewed

    J. Biochem.   138 ( 2 )   201 - 207   2005( ISSN:0021-924X

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1093/jb/mvi116

  • Functional expression of hexahistidine-tagged β-subunit of yeast F1-ATPase and isolation of the enzyme by immobilized metal affinity chromatography Reviewed

    Protein Expr. Purif.   37   97 - 101   2004( ISSN:1046-5928

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Overexpression, purification, and characterization of human and bovine mitochondrial ATPase inhibitors: comparison of the properties of mammalian and yeast ATPase inhibitors Reviewed

    J. Bioenerg. Biomembr.   35 ( 5 )   399 - 407   2003( ISSN:0145-479X

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • A yeast mitochondrial ATPase inhibitor interacts with three proteins that are easy to dissociate from the mitochondrial inner membrane Reviewed

    J. Biochem.   132 ( 4 )   649 - 654   2002( ISSN:0021-924X

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • The region from phenylalanine-17 to phenylalanine-28 of a yeast mitochondrial ATPase inhibitor is essential for its ATPase inhibitory activity Reviewed

    J. Biochem.   130 ( 5 )   687 - 693   2001( ISSN:0021-924X

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Deletion of mitochondrial ATPase inhibitor in yeast Saccharomyces cerevisiae decreased cellular and mitochondrial ATP levels under non-nutritional conditions and induced a respiration-deficient cell type Reviewed

    J. Biochem.   130 ( 6 )   873 - 878   2001

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Requirement for lysine-19 of the yeast mitochondrial ATPase inhibitor for the stability of the inactivated inhibitor-F1F0 complex at higher pH. Reviewed

    Biosci. Biotechnol. Biochem.   64 ( 1 )   89 - 95   2000

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Improved hypolipidemic effects of xanthan gum-galactomannan mixtures in rat Reviewed

    64 ( 10 )   2161 - 2167   2000

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Binding of an intrinsic ATPase inhibitor to the F1F0 ATPase in phosphorylating conditions of yeast mitochondria Reviewed

    J. Biochem.   128 ( 4 )   553 - 559   2000( ISSN:0021-924X

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Nucleotide sequence of cDNA coding the mitochondrial precursor protein of the ATPase inhibitor from humans Reviewed

    Biosci. Biotechnol. Biochem.   63 ( 12 )   2225 - 2227   1999

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • The carboxyl-terminal region of the yeast ATPase inhibitor is indispensable for the stability of the protein in mitochondria Reviewed

    J. Biochem.   124 ( 5 )   886 - 891   1998( ISSN:0021-924X

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • An intrinsic ATPase inhibitor binds near the active site of yeast mitochondrial F1F0-ATPase Reviewed

    J. Biochem.   119 ( 1 )   193 - 199   1996( ISSN:0021-924X

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Binding of an intrinsic ATPase inhibitor to the interface between α- and β- subunits of F1F0-ATPase upon de-Energization of mitochndria Reviewed

    J. Biochem.   113 ( 3 )   350 - 354   1993( ISSN:0021-924X

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Activation of ATP hydrolysis by an uncoupler in mutant mitochondria lacking an intrinsic ATPase inhibitor in yeast Reviewed

    J. Biol. Chem.   265 ( 11 )   6274 - 6278   1990( ISSN:0021-9258

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Inhibition of the uncoupler-induced mitochondrial ATP-hydrolysis by the cooperative work of the ATPase inhibitor, 9K protein and 15K protein

    Bioenergetics : Molecular Biology, Biochemistry and Pathology, Plenum Publishing Co. , New York   227 - 232   1990( ISBN:0-306-43673-6

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    Kind of work:Joint Work  

  • Isolation of a gene for a regulatory 15-kDa subunit of mitochondrial F1F0-ATPase and construction of mutant yeast lacking the protein Reviewed

    Eur. J. Biochem.   192   49 - 53   1990

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

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Books and Other Publications

  • 管理栄養士コースで学ぶ!~キャリアデザインのために~

    奥田豊子ほか編( Role: Joint author)

    同文書院  2007  ( ISBN:978-4-8103-1349-9

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    Book type:Scholarly book

  • 国家試験徹底分析 管理栄養士医学系集中講義 第22章 生体膜、第24章 エネルギー産生、第25章 糖質代謝

    松田覚編( Role: Joint author)

    金芳堂  2006  ( ISBN:4-7653-1279-8

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    Book type:Scholarly book

  • 廣川タンパク質化学7 制御タンパク質(インヒビター)「ATPaseインヒビター」

    市川直樹, 田川邦夫( Role: Joint author)

    廣川書店  2002  ( ISBN:4-567-16070-3

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    Book type:Scholarly book

  • 生活科学最前線90のトピック

    大阪市立大学生活科学部出版編集委員会編( Role: Joint author)

    中央法規  1999  ( ISBN:4-8058-1837-9

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    Book type:Scholarly book

Presentations

  • 酵母 ATP合成酵素 βサブユニット A475E変異の作製 Domestic conference

    市川 直樹, 福田 有紗, 西野 菜成子

    第94回日本生化学会大会(WEB開催)  2021.11  日本生化学会

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    Presentation type:Oral presentation (general)  

    Venue:WEB開催  

  • 酵母ATPaseインヒビター (IF1) のアルギニン20残基の必要性 Domestic conference

    市川直樹、五反田梨瑚、津田和奏

    第93回日本生化学会大会(WEB開催)  2020.09 

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    Presentation type:Oral presentation (general)  

  • 酵母ATP合成酵素γAsp241のIF1結合過程における役割の検証 Domestic conference

    市川直樹、服部碧、田中里穂、田之上豪

    第92回日本生化学会大会  2019.09 

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    Presentation type:Oral presentation (general)  

  • 酵母ATP合成酵素阻害タンパク質IF1のPhe17変異体の作製と解析 Domestic conference

    市川直樹、飯田聡美、後藤由加里、早見友希

    第91回日本生化学会大会  2018.09 

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    Presentation type:Poster presentation  

  • 酵母ATP合成酵素阻害タンパク質IF1のN末端欠失変異体の作製と機能解析 Domestic conference

    吉村優穂、市川直樹

    2017年度生命科学系学会合同年次大会(ConBio2017)  2017.12 

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    Presentation type:Poster presentation  

  • 酵母ATP合成酵素阻害タンパク質IF1のN末端欠失変異体の作製 Domestic conference

    吉村優穂、市川直樹

    第89回日本生化学会大会 in 仙台  2016.09 

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    Presentation type:Poster presentation  

  • N末端1-12領域を欠失する酵母IF1変異体の作製 Domestic conference

    許柏、市川直樹

    第38回日本分子生物学会年会、第88回日本生化学会大会合同大会(BMB2015)  2015.12 

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    Presentation type:Poster presentation  

  • 酵母IF1のαへリックス構造 Domestic conference

    孫麗、中前直美、市川直樹

    第87回日本生化学会大会  2014.10 

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    Presentation type:Poster presentation  

  • プロリン走査変異を導入したIF1を発現する酵母株の構築 Domestic conference

    孫麗、中前直美、市川直樹

    第86回日本生化学会大会  2013.09 

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    Presentation type:Poster presentation  

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Outline of education staff

  • Biochemistry

Charge of on-campus class subject

  • 生体エネルギー学特論

    2022   Weekly class   Graduate school

  • 生化学Ⅰ

    2022   Weekly class   Undergraduate

  • 生化学実験

    2022   Weekly class   Undergraduate

  • 初年次ゼミナール

    2022   Weekly class   Undergraduate

  • 健康へのアプローチ

    2022   Weekly class   Undergraduate

  • 生体エネルギー学特論演習A

    2022   Intensive lecture   Graduate school

  • 生体エネルギー学特論

    2022   Weekly class   Graduate school

  • 食栄養学概論

    2022   Weekly class   Undergraduate

  • 生化学実験

    2021     Undergraduate

  • Biochemistry I

    2021     Undergraduate

  • 卒業論文

    2021     Undergraduate

  • 食品栄養科学概論

    2021     Undergraduate

  • 前期特別研究

    2021     Graduate school

  • 生体エネルギー学特論

    2021     Graduate school

  • 生体エネルギー学特論演習

    2021     Graduate school

  • 基礎演習

    2021     Undergraduate

  • 食物学Ⅰ

    2021     Undergraduate

  • Biochemistry I

    2020     Undergraduate

  • 卒業論文

    2020     Undergraduate

  • 食品栄養科学概論

    2020     Undergraduate

  • 前期特別研究

    2020     Graduate school

  • 生体エネルギー学特論

    2020     Graduate school

  • 生体エネルギー学特論演習

    2020     Graduate school

  • 基礎演習

    2020     Undergraduate

  • 生化学実験

    2020     Undergraduate

  • 前期特別研究

    2019     Graduate school

  • 食品栄養科学概論

    2019     Undergraduate

  • 卒業論文

    2019     Undergraduate

  • 生体エネルギー学特論

    2019     Graduate school

  • 生体エネルギー学特論演習

    2019     Graduate school

  • 基礎演習

    2019     Undergraduate

  • 食物学Ⅰ

    2019     Undergraduate

  • 生化学実験

    2019     Undergraduate

  • Biochemistry I

    2019     Undergraduate

  • 前期特別研究

    2018     Graduate school

  • 健康へのアプローチ

    2018     Undergraduate

  • 食品栄養科学概論

    2018     Undergraduate

  • 卒業論文

    2018     Undergraduate

  • 生体エネルギー学特論

    2018     Graduate school

  • 生体エネルギー学特論演習

    2018     Graduate school

  • 基礎演習

    2018     Undergraduate

  • 食物学Ⅰ

    2018     Undergraduate

  • 生化学実験

    2018     Undergraduate

  • Biochemistry I

    2018     Undergraduate

  • 前期特別研究

    2017     Graduate school

  • 健康へのアプローチ

    2017     Undergraduate

  • 食品栄養科学概論

    2017     Undergraduate

  • 卒業論文

    2017     Undergraduate

  • 化学実験

    2017     Undergraduate

  • 生体エネルギー学特論

    2017     Graduate school

  • 生体エネルギー学特論演習

    2017     Graduate school

  • 基礎演習

    2017     Undergraduate

  • 食物学Ⅰ

    2017     Undergraduate

  • 生化学実験

    2017     Undergraduate

  • Biochemistry I

    2017     Undergraduate

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Faculty development activities

  • FD研究会発表  2003

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    タイトル「講義科目『生化学』の授業での試み」第1回大阪市立大学FD研究会(2003年)口頭発表

Other

  • Job Career

    2022.04 - Now

  • Job Career

    2007.04 - 2022.03