Updated on 2023/03/29

写真a

 
MIYAHARA Ikuko
 
Organization
Graduate School of Science Department of Chemistry Associate Professor
School of Science Department of Chemistry
Title
Associate Professor
Affiliation
Institute of Science

Position

  • Graduate School of Science Department of Chemistry 

    Associate Professor  2022.04 - Now

  • School of Science Department of Chemistry 

    Associate Professor  2022.04 - Now

Degree

  • Doctor of Science ( Others )

  • Master of Science ( Osaka City University )

Research Areas

  • Life Science / Structural biochemistry  / Structured Biochemistry

  • Nanotechnology/Materials / Fundamental physical chemistry  / Physical Chemistry

Research Career

  • X-ray crystal structure analysis of Proteins

    X-ray structure analysis, enzyme  Joint Research in Japan

    1994 - Now 

  • Structural Chemistry of Organic and Organometallic Compound

    Organic crystal, X-ray structure analysis  Joint Research in Japan

    1990 - Now 

Professional Memberships

  • The Crystallographic Society of Japan

      Domestic

  • 日本蛋白質科学会

      Domestic

  • The Chemical Society of Japan

      Domestic

  • The Japanese Biochemical Society

      Domestic

  • The Vitamin Society of Japan

Committee Memberships (off-campus)

  • 評議員   日本結晶学会  

    2021.04 - Now 

  • 評議員   アジア結晶学会  

    2020.04 - Now 

  • 編集委員   日本結晶学会  

    2020.04 - 2022.03 

  • プログラム委員   第16回アジア結晶学会  

    2019 

  • プログラム委員   日本結晶学会2019年度年会  

    2019 

  • 編集委員長   日本結晶学会  

    2018.04 - 2020.03 

  • プログラム委員   日本結晶学会2018年度年会  

    2018 

  • 代議員   日本ビタミン学会  

    2016.04 - Now 

  • 評議員   日本結晶学会  

    2014.04 - 2020.03 

  • 編集委員   日本結晶学会  

    2002.04 - 2018.03 

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Education

  • Osaka City University   Master's Course  

    - 1993

  • Osaka City University    

    - 1991

Papers

  • Crystal structure of Sphingobacterium multivorum serine palmitoyltransferase complexed with tris(hydroxymethyl)aminomethane

    Ikushiro H.

    Acta Crystallographica Section F: Structural Biology Communications   78   408 - 415   2022.11

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  • The crystal structure of D-amino acid oxidase with a substrate analog, o-aminobenzoate

    Miyahara I.

    Journal of Biochemistry   171 ( 1 )   27 - 29   2022.01( ISSN:0021924X

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  • Structural Analysis of Serin Hydroxymethyltransferase in Complex with Substrate-analogue Reviewed

    Yukawa Naoki, Akai Shota, Kamiya Nobuo, Miyahara Ikuko

    Japan Synchrotron Radiation Research Institute, SPring-8/SACLA Research Report   9 ( 5 )   275 - 277   2021

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.18957/rr.9.5.275

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  • The crystal structure of homoserine dehydrogenase complexed with L-homoserine and NADPH in a closed form. Reviewed

    Akai S, Ikushiro H, Sawai T, Yano T, Kamiya N, Miyahara I

    Journal of biochemistry   165 ( 2 )   185 - 195   2019.02( ISSN:0021-924X

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/jb/mvy094

    PubMed

  • L-ホモセリンおよびNADPHとclosed formで複合体形成したホモセリン・デヒドロゲナーゼの結晶構造(The crystal structure of homoserine dehydrogenase complexed with L-homoserine and NADPH in a closed form) Reviewed

    Akai Shota, Ikushiro Hiroko, Sawai Taiki, Yano Takato, Kamiya Nobuo, Miyahara Ikuko

    (公社)日本生化学会 The Journal of Biochemistry   165 ( 2 )   185 - 195   2019.02( ISSN:0021-924X

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    Publishing type:Research paper (scientific journal)  

    L-ホモセリン(L-Hse)とNAD(P)Hからなる非リガンド性Thermus thermophilusのホモセリン・デヒドロゲナーゼ(TtHSD)、ならびにLys99AlaとLys195Alaが変異したTtHSDの結晶構造を、それぞれ1.83、2.00、1.87および1.93Åで解析した。L-HseとNADPHの結合はTtHSDの構造をopenからclosedに変化させ、Glu180を含む可動ループが近づいて両者を固定した。Lys99AlaとLys195AlaはいずれもL-Hseの水酸基と水素結合を形成した。TtHSD三重複合体は、closed型の方がopen型より良好にMichaelis複合体を模倣した。Lys99Ala TtHSDの結晶構造はLys99関連水素結合に関与する水分子を含むが、Lys195とL-Hseの位置は維持されていた。以上より、Lys99がHSDの酸-塩基触媒の残基である可能性が考えられた。

  • Structural Investigation on the ES Complex of D-amino Acid Oxidase By using Artificial FAD Reviewed

    Miyahara Ikuko, Setoyama Chiaki, Nishina Yasuzo

    Japan Synchrotron Radiation Research Institute, SPring-8/SACLA Research Report   7 ( 1 )   13 - 15   2019

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.18957/rr.7.1.13

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  • Heme-dependent Inactivation of 5-Aminolevulinate Synthase from Caulobacter crescentus. Reviewed

    Ikushiro H, Nagami A, Takai T, Sawai T, Shimeno Y, Hori H, Miyahara I, Kamiya N, Yano T

    Scientific reports   8 ( 1 )   14228   2018.09( ISSN:2045-2322

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41598-018-32591-z

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  • ADP-Ribose Pyrophosphatase Reaction in Crystalline State Conducted by Consecutive Binding of Two Manganese(II) Ions as Cofactors Reviewed

    Furuike Yoshihiko, Akita Yuka, Miyahara Ikuko, Kamiya Nobuo

    BIOCHEMISTRY   55 ( 12 )   1801 - 1812   2016.03( ISSN:0006-2960

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/acs.biochem.5b00886

  • 5.アスパラギン酸アミノトランスフェラーゼとマロン酸の複合体による超閉構造について(第442回研究協議会研究発表要旨,ビタミンB研究委員会) Reviewed

    村川 武志, 宮原 郁子, 林 秀行

    公益社団法人 日本ビタミン学会 ビタミン   90 ( 1 )   43 - 44   2016

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.20632/vso.90.1_43

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  • Observation of ADP-Ribose Pyrophosphatase Reaction in Crystalline-State Induced by Mn(II)Ion Binding Reviewed

    FURUIKE Yoshihiko, MIYAHARA Ikuko, KAMIYA Nobuo

    The Crystallographic Society of Japan, Nihon Kessho Gakkaishi   58 ( 4 )   186 - 192   2016( ISSN:0369-4585

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    Publishing type:Research paper (scientific journal)  

    <p>ADP-Ribose pyrophosphatase (ADPRase) reaction was observed in crystalline-state at atomic resolutions around 1 Å. ADPRase achieves the general acid-base catalysis in the presence of divalent metal cations. Crystals of ADPRase alone, ADPRase-ADPR binary (ES-state) complex were prepared, and reaction intermediates were captured by the cryo-trapping after the soaking of MnCl<sub>2</sub> into ES-state crystals. Just after two metal binding sites were successively occupied along with the soaking time, ADPR was hydrolyzed and reaction products were released, indicating the completion of the crystalline-state reaction. The key structures of ADPRase-ADPR-two Mn<sup>2+</sup> ions complex revealed the various enzymatic phenomena including electrostatic, proximity and orientation effects and preferential binding for the transition states. The details of the metal assisting mechanism and the general base catalysis have been discussed.</p>

    DOI: 10.5940/jcrsj.58.186

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  • A new member of MocR/GabR-type PLP-binding regulator of d-alanyl-d-alanine ligase in Brevibacillusbrevis Reviewed

    Takenaka Takashi, Ito Tomokazu, Miyahara Ikuko, Hemmi Hisashi, Yoshimura Tohru

    FEBS JOURNAL   282 ( 21 )   4201 - 4217   2015.11( ISSN:1742-464X

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/febs.13415

  • Proton Transfer through Function-Unknown Glu residue Highly Conserved in ADPRase Reviewed

    Furuike Y., Akita Y., Tomita Y., Miyahara I., Kamiya N.

    ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES   70   C1209 - C1209   2014.08( ISSN:2053-2733

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  • Crystallography in Biology; Visualization of Biological Process Reviewed

    MIYAHARA Ikuko, IGARASHI Noriyuki, TANAKA Nobutada, KURISU Genji

    The Crystallographic Society of Japan, Nihon Kessho Gakkaishi   56 ( 4 )   223 - 223   2014( ISSN:0369-4585

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.5940/jcrsj.56.223

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  • Book Review Reviewed

    MIYAHARA Ikuko

    The Crystallographic Society of Japan, Nihon Kessho Gakkaishi   56 ( 6 )   422 - 422   2014( ISSN:0369-4585

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    Publishing type:Research paper (scientific journal)   Kind of work:Single Work  

    DOI: 10.5940/jcrsj.56.422

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  • Crystallographic study of homoserine dehydrogenase from Thermus thermophilus HB8 Reviewed

    Akai Shota, Ikushiro Hiroko, Sawai Daiki, Hayashi Hideyuki, Kamiya Nobuo, Miyahara Ikuko

    THE VITAMIN SOCIETY OF JAPAN, VITAMINS   88 ( 7 )   358 - 365   2014( ISSN:0006-386X

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    Publishing type:Research paper (scientific journal)  

    Homoserine dehydrogenase catalyzes the conversion of L-aspartate-4-semialdehyde to L-homoserine. Homoserine dehydrogenase is required for the biosynthesis of the three essential amino acids, i.e. lysine, methionine, and isoleucine, from aspartic acid. This enzyme attracts attention also as a promising antifungal drug target. We have determined the crystal structures of homoserine dehydrogenase from Thermus thermophilus HB8 in both substrate-free form and homoserine-binding form by X-ray diffraction. Crystallization conditions were surveyed at 293 K by using a hanging-drop vapor-diffusion method. The substrate-free form was solved to 1.4 Å resolution and the homoserine-binding form was solved to 2.0 Å resolution. The homoserine dehydrogenase was a dimer with each subunit composed of three distinct domains, nucleotide-binding, dimerization, and substrate-binding domain. In the homoserine-binding forms, the amino group of homoserine made a hydrogen bond to the side chain carboxylate of Glu180. This hydrogen bond induced the conformation change of Thr165-Pro183 loop, which made the loop close to the active site. The substrate homoserine was rigidly recognized by several amino acid residues in the enzyme active site, indicating that Lys99 or Lys195 would be an essential catalytic residue to facilitate hydride transfer.

    DOI: 10.20632/vso.88.7_358

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  • Protonation states of key residues observed during in situ ADPRase reaction Reviewed

    Furuike Yoshihiko, Akita Yuka, Miyahara Ikuko, Kamiya Nobuo

    ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES   67   C788 - C788   2011( ISSN:2053-2733

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  • Reaction Mechanism and Molecular Basis for Selenium/Sulfur Discrimination of Selenocysteine Lyase Reviewed

    Omi Rie, Kurokawa Suguru, Mihara Hisaaki, Hayashi Hideyuki, Goto Masaru, Miyahara Ikuko, Kurihara Tatsuo, Hirotsu Ken, Esaki Nobuyoshi

    JOURNAL OF BIOLOGICAL CHEMISTRY   285 ( 16 )   12133 - 12139   2010.04( ISSN:0021-9258

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M109.084475

  • Structural Insights into the Enzymatic Mechanism of Serine Palmitoyltransferase from Sphingobacterium multivorum Reviewed

    Ikushiro Hiroko, Islam Mohammad Mainul, Okamoto Akihiro, HOSEKI Jun, MURAKAWA Takeshi, FUJII Shigeru, MIYAHARA Ikuko, HAYASHI Hideyuki

    Japanese Biochemical Society The journal of biochemistry   146 ( 4 )   549 - 562   2009.10( ISSN:0021924X

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  • Crystal Structure of a Homolog of Mammalian Serine Racemase from Schizosaccharomyces pombe Reviewed

    Goto Masaru, Yamauchi Takae, Kamiya Nobuo, Miyahara Ikuko, Yoshimura Tohru, Mihara Hisaaki, Kurihara Tatsuo, Hirotsu Ken, Esaki Nobuyoshi

    JOURNAL OF BIOLOGICAL CHEMISTRY   284 ( 38 )   25944 - 25952   2009.09( ISSN:0021-9258

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M109.010470

  • X-Ray Crystallographic and Mutational Studies of Fluoroacetate Dehalogenase from Burkholderia sp Strain FA1 Reviewed

    Jitsumori Keiji, Omi Rie, Kurihara Tatsuo, Kurata Atsushi, Mihara Hisaaki, Miyahara Ikuko, Hirotsu Ken, Esaki Nobuyoshi

    JOURNAL OF BACTERIOLOGY   191 ( 8 )   2630 - 2637   2009.04( ISSN:0021-9193

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1128/JB.01654-08

  • Serine Racemase with Catalytically Active Lysinoalanyl Residue Reviewed

    Yamauchi Takae, Goto Masaru, Wu Hui-Yuan, UO Takuma, YOSHIMURA Tohru, MIHARA Hisaaki, KURIHARA Tatsuo, MIYAHARA Ikuko, HIROTSU Ken, ESAKI Nobuyoshi

    Japanese Biochemical Society The journal of biochemistry   145 ( 4 )   421 - 424   2009.04( ISSN:0021924X

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  • Time-programmed peptide helix inversion of a synthetic metal complex triggered by an achiral NO3- anion Reviewed

    Miyake Hiroyuki, Kamon Hiroshi, Miyahara Ikuko, Sugimoto Hideki, Tsukube Hiroshi

    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   130 ( 3 )   792 - +   2008.01( ISSN:0002-7863

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/ja0768385

  • 2.アスパラギン酸アミノ基転移酵素とグルタミン酸の反応の解析 : C4,C5基質の反応の比較(第410回研究協議会研究発表要旨,ビタミンB研究委員会) Reviewed

    林 秀行, 生城 浩子, 後藤 勝, 広津 建, 宮原 郁子

    公益社団法人 日本ビタミン学会 ビタミン   82 ( 1 )   2008

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.20632/vso.82.1_60

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  • Structure of membrane-bound quiohemoprotein alcohol dehydrogenase Reviewed

    Goto Masaru, Miyahara Ikuko, Hirotsu Ken, Kobayashi Yoshiki, Nakatsuka Tomoko, Toyama Hirohide, Adachi Osao, Matsushita Kazunobu

    ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES   64   C242 - C242   2008( ISSN:2053-2733

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  • Structural study of putative aminotransferase from Thermus thermophilus HB8 Reviewed

    Miyahara Ikuko, Matsumura Mitsuyoshi, Goto Masaru, Omi Rie, Hirotsu Ken, Mizuguchi Hiroyuki, Hayashi Hideyuki

    ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES   64   C280 - C281   2008( ISSN:2053-2733

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  • Reaction pathway of ADP-ribose pyrophosphatase, revealed by time-resolved X-ray crystallography Reviewed

    Kamiya Nobuo, Kai Kentaro, Nakagawa Noriko, Kuramitsu Seiki, Miyahara Ikuko

    ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES   64   C271 - C272   2008( ISSN:2053-2733

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  • 5.ビタミンB_6酵素の活性中心構造と機能 : フレキシブルなループによる誘導適合(B群ビタミン酵素構造機能解析の新展開,シンポジウム(平成19年度),ビタミンB研究委員会) Reviewed

    宮原 郁子

    公益社団法人 日本ビタミン学会 ビタミン   82 ( 3 )   215 - 216   2008

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    Publishing type:Research paper (scientific journal)   Kind of work:Single Work  

    DOI: 10.20632/vso.82.3_215

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  • Crystal structure of monofunctional histidinol phosphate phosphatase from Thermus thermophilus HB8 Reviewed

    Omi Ric, Goto Masaru, Miyahara Ikuko, Manzoku Miho, Ebihara Akio, Hirotsu Ken

    BIOCHEMISTRY   46 ( 44 )   12618 - 12627   2007.11( ISSN:0006-2960

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/bi701204r

  • Expression, purification and preliminary X-ray characterization of DL-2-haloacid dehalogenase from Methylobacterium sp CPA1 Reviewed

    Omi Rie, Jitsumori Keiji, Yamauchi Takahiro, Ichiyama Susumu, Kurihara Tatsuo, Esaki Nobuyoshi, Kamiya Nobuo, Hirotsu Ken, Miyahara Ikuko

    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS   63   586 - 589   2007.07( ISSN:2053-230X

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1107/S1744309107027273

  • Structural Study of Selenocysteine Lyase Reviewed

    Omi Rie, Kurokawa Suguru, Mihara Hisaaki, Kurihara Tatsuo, Esaki Nobuyoshi, Hirotsu Ken, Miyahara Ikuko

    ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES   63   S121 - S121   2007( ISSN:2053-2733

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1107/S0108767307097383

  • Crystal structures of nonoxidative zinc-dependent 2,6-dihydroxybenzoate (gamma-resorcylate) decarboxylase from Rhizobium sp strain MTP-10005 Reviewed

    Goto Masaru, Hayashi Hideyuki, Miyahara Ikuko, Hirotsu Ken, Yoshida Masahiro, Oikawa Tadao

    JOURNAL OF BIOLOGICAL CHEMISTRY   281 ( 45 )   34365 - 34373   2006.11( ISSN:0021-9258

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M607270200

  • Engineering the Substrate Specificity of Porcine Kidney D-Amino Acid Oxidase by Mutagenesis of the "Active-Site Lid" Reviewed

    Setoyama Chiaki, Nishina Yasuzo, Mizutani Hisashi, MIYAHARA Ikuko, HIROTSU Ken, KAMIYA Nobuo, SHIGA Kiyoshi, MIURA Retsu

    Japanese Biochemical Society The journal of biochemistry   139 ( 5 )   873 - 879   2006.05( ISSN:0021924X

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  • Three-Dimensional Structure of Rat-Liver Acyl-CoA Oxidase in Complex with a Fatty Acid : Insights into Substrate-Recognition and Reactivity toward Molecular Oxygen Reviewed

    Tokuoka Keiji, Nakajima Yoshitaka, Hirotsu Ken, MIYAHARA Ikuko, NISHINA Yasuzo, SHIGA Kiyoshi, TAMAOKI Haruhiko, SETOYAMA Chiaki, TOJO Hiromasa, MIURA Retsu

    Japanese Biochemical Society The journal of biochemistry   139 ( 4 )   789 - 795   2006.04( ISSN:0021924X

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  • Crystal Structures of Nonoxidative Zinc-dependent 2,6-duhidroxybenzoate (g -resorcylate) Decarboxylase from Rhizobium sp. Strain MTP-100005.

    J. Biol. Chem   281   34365 - 34373   2006

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  • Engineering the Substrate Specificity of Porcine Kidney D-Amino Acid Oxidase by Mutagenesis of the "Active-Site Lid

    J. Biochem   139   8730879   2006

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  • Three-dimensional Structure of Rat-liver Acyl-CoA Oxidase in Complex with a Fatty Acid: Insights into Substrate-recognition and Reactivity Toward Molecular Oxygen.

    J. Biochem   139   7890795   2006

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    Publishing type:Research paper (scientific journal)  

  • Precise Stereostructure and Fucntion of Vitamin B_2-or B_6-Dependent Enzymes Reviewed

    Miyahara Ikuko

    THE VITAMIN SOCIETY OF JAPAN, VITAMINS   80 ( 3 )   115 - 123   2006( ISSN:0006386X

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    Publishing type:Research paper (scientific journal)   Kind of work:Single Work  

    DOI: 10.20632/vso.80.3_115

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  • Three-Dimensional Structure of Rat-Liver Acyl-CoA Oxidase in Complex with Fatty Acid Reviewed

    Miyahara Ikuko, Nakajima Yoshitaka, Tokuoka Keiji, Hirotsu Ken, Nishina Yasuzo, Shiga Kiyoshi, Setoyama Chiaki, Tamaoki Haruhiko, Tojo Hiromasa, Miura Retsu

    ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES   62   S153 - S153   2006( ISSN:2053-2733

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1107/S0108767306096942

  • Crystal structures of Delta(1)-piperideine-2-carboxylate/Delta(1)-pyrroline-2-carboxylate reductase belonging to a new family of NAD(P)H-dependent oxidoreductases - Conformational change, substrate recognition, and stereochemistry of the reaction Reviewed

    Goto M, Muramatsu H, Mihara H, Kurihara T, Esaki N, Omi R, Miyahara I, Hirotsu K

    JOURNAL OF BIOLOGICAL CHEMISTRY   280 ( 49 )   40875 - 40884   2005.12( ISSN:0021-9258

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  • Crystal structures of Delta1-piperideine-2-carboxylate/Delta1-pyrroline-2-carboxylate reductase belonging to a new family of NAD(P)H-dependent oxidoreductases: conformational change, substrate recognition, and stereochemistry of the reaction. Reviewed

    Goto M, Muramatsu H, Mihara H, Kurihara T, Esaki N, Omi R, Miyahara I, Hirotsu K

    The Journal of biological chemistry   280 ( 49 )   40875 - 40884   2005.12( ISSN:0021-9258

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M507399200

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  • A New Family of NAD(P)H-Dependent Oxidoreductases Distinct from Conventional Rossmann-Fold Proteins Reviewed

    Muramatsu Hisashi, Mihara Hisaaki, Goto Masaru, MIYAHARA IKUKO, HIROTSU KEN, KURIHARA TATSUO, ESAKI NOBUYOSHI

    公益社団法人日本生物工学会 Journal of bioscience and bioengineering   99 ( 6 )   541 - 547   2005.06( ISSN:13891723

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    A new family of NAD(P)H-dependent oxidoreductases is now recognized as a protein family distinct from conventional Rossmann-fold proteins. Numerous putative proteins belonging to the family have been annotated as malate dehydrogenase (MDH) or lactate dehydrogenase (LDH) according to the previous classification as type-2 malate/L-lactate dehydrogenases. However, recent biochemical and genetic studies have revealed that the protein family consists of a wide variety of enzymes with unique catalytic activities other than MDH or LDH activity. Based on their sequence homologies and plausible functions, the family proteins can be grouped into eight clades. This classification would be useful for reliable functional annotation of the new family of NAD(P)H-dependent oxidoreductases.

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  • Binding of C5-dicarboxylic substrate to aspartate aminotransferase: Implications for the conformational change at the transaldimination step Reviewed

    Islam MM, Goto M, Miyahara I, Ikushiro I, Hirotsu K, Hayashi H

    BIOCHEMISTRY   44 ( 23 )   8218 - 8229   2005.06( ISSN:0006-2960

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/bi050071g

  • ビタミンB_2およびB_6依存性酵素の立体構造と機能の精密解析 Reviewed

    宮原 郁子

    ビタミン =VITAMINS   79 ( 4 )   2005.04( ISSN:0006386X

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    CiNii Article

  • Structural determinants for branched-chain aminotransferase isozyme specific inhibition by the anticonvulsant drug gabapentin.

    J. Biol. Chem.   280 ( 44 )   37246 - 37256   2005

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  • Dual substrate recognition of aminotransferases Reviewed

    Hirotsu K, Goto M, Okamoto A, Miyahara I

    CHEMICAL RECORD   5 ( 3 )   160 - 172   2005( ISSN:1527-8999

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/tcr.20042

  • Structural Basis for the Induced Fit, Substrate Recognition, and Mechanism of Threonine Synthase Reviewed

    Omi Rie, Goto Masaru, Miyahara Ikuko, Hirotsu Ken

    ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES   61   C199 - C200   2005( ISSN:2053-2733

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  • Dual Substrate Recognition of Acetylornithine Aminotransferase Reviewed

    Miyahara Ikuko, Matsumura Mitsuyoshi, Goto Masaru, Omi Rie, Hirotsu Ken, Mizuguchi Hiroyuki, Hayashi Hideyuki, Kagamiyama Hiroyuki

    ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES   61   C198 - C198   2005( ISSN:2053-2733

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    Publishing type:Research paper (scientific journal)  

  • Crystal Structures of Serine Racemase from S. pombe Reviewed

    Goto Masaru, Omi Rie, Miyahara Ikuko, Hirotsu Ken

    ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES   61   C199 - C199   2005( ISSN:2053-2733

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    Publishing type:Research paper (scientific journal)  

  • ビタミンB_2およびB_6依存性酵素の立体構造と機能の精密解析(奨励賞)(受賞講演)(第57回大会研究発表要旨) Reviewed

    宮原 郁子

    公益社団法人 日本ビタミン学会 ビタミン   79 ( 4 )   2005( ISSN:0006-386X

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    Publishing type:Research paper (scientific journal)   Kind of work:Single Work  

    DOI: 10.20632/vso.79.4_219

    CiNii Article

  • Expression, purification and preliminary X-ray characterization of N-acetyl-γ-glutamil-phosphate reductase from Thermus thermophilus HB8

    Acta CrystD   59   356 - 358   2003

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    Publishing type:Research paper (scientific journal)  

  • Structure of Imidazole Glycerol Phosphate Synthase from Thermus thermophilus HB8: Open-Closed Conformational Change and Ammonia Tunneling

    J. Biochem   132 ( 5 )   759 - 765   2002

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    Publishing type:Research paper (scientific journal)  

  • Three-dimensional Structure of the Flavoenzyme Acyl-CoA Oxidase-II from Rat Liver, the Peroxisomal Counterpart of Mitochondrial Acyl-CoA Dehydrogenase

    J. Biochem   131   367 - 374   2002

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    Publishing type:Research paper (scientific journal)  

  • Structure of Esherichia coli Histidinol-Phosphate Aminotransferase and Its Complexes with Hitidinol-Phosphate and N-(5'-Phosphopyridoxyl)-L-Glutamate: Double Substrate Recognition of the Enzyme

    Biochemistry   40 ( 15 )   4633 - 4644   2001

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    Publishing type:Research paper (scientific journal)  

  • Three-Dimensional Structure of 4-Amino-4-deoxychorismate Lyase from Escherichia coli.

    J. Biochemistry   ( 128 )   29 - 38   2000

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    Publishing type:Research paper (scientific journal)  

  • Stereochemistry of the Transamination Reaction Catalyzed by Aminodeoxychorismate Lyase from Escherichia coli: Close Relationship between Fold Type and Stereochemistry

    J. Biochemistry   ( 128 )   679 - 686   2000

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    Publishing type:Research paper (scientific journal)  

  • Khayanolides, rearranged phragmalin limonoid antifeedants from Kyhaya senegalensis

    Tetrahedron   ( 57 )   119 - 126   2000

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    Publishing type:Research paper (scientific journal)  

  • Annew host 2,3,6,7,10,11-hexahydroxy triphenylene which forms chiral inclusion lattice: X-ray structural study of chiral crystalline lattice.

    J. Phys. Org. Chem.   ( 13 )   39 - 45   2000

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    Publishing type:Research paper (scientific journal)  

  • Three-Dimensional Structure of the Purple Intermediate of Porcine Kidney D-Amino Acid Oxidase. Optimization of the Oxidative Half-Reaction through Alignment of the Product with Reduced Flavin.

    J. Biochemistry   ( 128 )   73 - 81   2000

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    Publishing type:Research paper (scientific journal)  

  • Enantioselective Single-Crystal-to-Single-Crystal Photodimerization of Coumarin and Thiocoumarin in Inclusion Complexes with Chiral Host Compounds

    Angew. Chem. Int. Ed   38 ( 23 )   3523 - 3525   1999

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    Publishing type:Research paper (scientific journal)  

  • The reaction scheme for D-amino acid oxidase: Substrate/product alignment for optimization of reductive and oxidative half-reactions

    Agency for Scientific Publ., Berlin, Flavins and Flavoproteins 1999   543 - 550   1999

  • Extermely Long C-C Bond in(-)-trans-1,2-Di-tert-butyl-1,2-diphenyl-and 1,1-Di-tert-butyl-2,2-diphenyl-3,8-dichlorocyclobuta[6] naphthalenes

    J. Org. Chem.   ( 64 )   3102 - 3105   1999

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    Publishing type:Research paper (scientific journal)  

  • Single crystal to single crystal enantioselective [2+2] photodimerization of cyclohex-2-enone as an inclusion complex with (-)-1,4-bis[3-(o-chlorophenyl)-3- hydroxy-3-phenylprop-1-ynyl]benzene

    Cryst.Eng.Comm.   3   1999

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  • Role of the hydrogen-bonding network associated with isoalloxazine in the catalysis of D-amino acid oxidase.

    Agency for Scientific Publ., Berlin, Flavins and Flavoproteins 1999   95 - 98   1999

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Books and Other Publications

  • タンパク質工学 = Protein Engineering

    老川 典夫, 大島 敏久, 保川 清, 三原 久明, 宮原 郁子( Role: Sole author)

    講談社  2018  ( ISBN:9784061538993

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    Book type:Scholarly book

    CiNii Books

  • Dual substrate recognition of aminotransferases

    ( Role: Joint author)

    Chem. Rec  2005 

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    Responsible for pages:3  

  • Crystal Structure of Glutamine:Phenylpyrvate Aminotransferase from Thermus thermophilus HB8: INDUCED FIT AND SUBSTRATE RECOGNITION

    ( Role: Joint author)

    J. Biol. Chem.  2004 

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    Responsible for pages:16  

  • Expression, purification and preliminary X-ray characterization of histidinol phosphate phosphatase

    ( Role: Joint author)

    Acta Crystallographica  2004 

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    Responsible for pages:3  

  • Silicon-Carbon Unsaturated Compounds. 69. Reactions of silenes produced thermally from pivaloyl- and admantoyltris(trimethylsilyl)silane with silyl-substituted butadienes and enynes.

    ( Role: Joint author)

    Organometallics  2004 

  • Stereochemistry of disilanylene-containing cyclic compounds. Synthesis and reactions of cis- and trans-3,4-benzo-1,2-di(tert-butyl)-1,2-dimethyl-1,2- disilacyclobut-3-ene.

    ( Role: Joint author)

    Organometalics  2004 

  • Crystal Structures of CTP synthetase reveal ATP, UTP, and glutamine binding sites

    ( Role: Joint author)

    Structure  2004 

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    Responsible for pages:8  

  • Expression, purification and preliminary X-ray characterization of CTP synthetase from Thermus thermophilus HB8

    ( Role: Joint author)

    Acta Cryst  2003 

  • Characterization of histidinol phosphate aminotransferase from Escherichia coli.

    ( Role: Joint author)

    Biochem. Biophys. Acta,  2003 

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    Responsible for pages:1-2  

  • Structures of Argininosuccinate Synthetase in Enzyme-ATP-Substrates and Enzyme-AMP-Product Forms: Stereochemistry of the Catalytic Reaction.

    ( Role: Joint author)

    J. Biol. Chem  2003 

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    Responsible for pages:25  

  • Crystal Structures of Threonine Synthase from Thermus thermophilus HB8: Conformational change, substrate recognition, and mechanism

    ( Role: Joint author)

    J. Biol. Chem  2003 

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    Responsible for pages:46  

  • Glutamine:phenylpyruvate Aminotransferase from an Extremely Thermophilic Bacterium Thermus thermophilus HB8

    ( Role: Joint author)

    J. Biochem  2003 

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    Responsible for pages:5  

  • Structure of Transition State Analog of Medium-Chain Acyl-CoA Dehydrogenase. Crystallographic and Molucular Orbital Studies on the Charge-Transfer Complex of Medium Acyl-CoA Dehydrogenase with 3-Thiaoctanoyl-CoA

    ( Role: Joint author)

    J. Biochem  2003 

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    Responsible for pages:25  

  • Strain and catalysis in aspartate amionotransferase

    ( Role: Joint author)

    Biochem. Biophys. Acta,  2003 

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    Responsible for pages:102  

  • Conformational change in aspartate aminotransferase on substrate binding induces strain in the catalytic group and enhances catalysis

    ( Role: Joint author)

    J. Biol. Chem  2003 

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    Responsible for pages:11  

  • Crystal structures of branched-chain amino acid aminotransferase complexed with glutamate and glutarate: true reaction intermediate and double substrate recognition of the enzyme.

    ( Role: Joint author)

    Biochemistry  2003 

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    Responsible for pages:13  

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MISC

  • Caulobacter crescentus由来5-アミノレブリン酸合成酵素において観察されたヘム依存性不活化現象 Reviewed

    生城 浩子, 長見 篤, 高井 智子, 澤井 大樹, 主馬野 祐希, 堀 洋, 宮原 郁子, 神谷 信夫, 矢野 貴人

    (公社)日本ビタミン学会 ビタミン   93 ( 12 )   542 - 544   2019.12( ISSN:0006-386X

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  • Structure of Thermus thermophilus HB8 Aspartate Aminotrans-ferase and Its Complex with Maleate

    Biochemistry   38 ( 8 )   2413 - 2424   1999

  • Superoxide Dismutase Mimetic Comples of Mn(Ⅱ)/N, N-Bis(2-Prytidylmethyl)-(S)-Histidine

    Chem, Lett.   295   1999

  • The Birch Reduction of Heterocyclic Compounds (]G0004[)[1], Birch Reduction of 3-Acylfurans. Competition between Ring Reduction, Carbonyl Reduction, and Dimer Formation

    J. Heterocyclic Chem.   ( 35 )   1285   1998

  • Potential Host Compounds : 1, 1"-Bis(diaryl hydroxymethyl)ferrocenes((Multiple Authorship))

    DBJ. Chem. Soc. Perkin. Trans. 2.(/)-DB   85 - 88   1997

  • Structural and Mechanistic Studies on D-Amino Acid Oxidase//Substrate Complex : Implications of the Crystal Structure of Enzyme//Substrate Analog Complex. ((Multiple Authorship))

    DBJ. Biochem.(/)-DB   122   825 - 833   1997

  • Photoreactivities of Two Kinds of Biomolecular Crystals Formed from Acidine and Pherothiazine((Multiple Authorship))

    DBJ. Chem. Soc. , Perkin Trans. 2(/)-DB   2033 - 2038   1997

  • Stoichiometrically Sensitized Decarboxylation Occurring in the Two Component Molecular Crystals of Aza Aromatic Compounds and Aralkyl Carboxylic Acids((Multiple Authorship))

    DBJ. Am. Chem. Soc.(/)DB   119   10317 - 10324   1997

  • Use of D21D2H-D115D1N Heteronuclear Multiple-Quantum Coherence NMR Spectroscopy To Study the Active Site of Aspartate Aminotransferase((Multiple Authorship))

    DBBiochemistry(/)-DB   36 ( 3 )   615 - 625   1997

  • Structural and Mechanistic Studies on D-Amino Acid Oxidase//Substrate Complex ; Implications of the Crystal Structure of Enzyme//Substrate Analog Complex

    J. Biochem.   ( 122 )   825 - 833   1997

  • Room Temperature Stable Triplet Carbene as Model for Organic High-Spin Units with Robust π-Spin Polarization ; ESR Study and X-Ray Analysis

    Mol. Cryst. Liq. Cryst.   ( 301 )   185 - 191   1997

  • Formation Racemic Compound Crystals by Mixing of Two Enantiomeric Crystals in the Solid State Liquid Transport of Molecules from Crystal to Crystal((Multiple Authorship))

    DBJ. Chem. Soc. , Perkin. Trans, 2(/)DB   1877 - 1885   1997

  • Design of New Host Compounds, 1, 3-Diphenyl-Cyclohexane-1, 3-Dial, 1, 4-, 1, 3-and 1, 2-Bis(hydroxydiphenylmethyl)cyclohexanes, and 1, 2-Bis(hydroxydiphenylmethyl)cyclopropane. ((Multiple Authorship))

    DBSupramolecular Chem.(/)-DB   ( 8 )   113 - 124   1997

  • Enantioselective Inclusion Complexation by the Chiral Host and Photocyclization Reaction. ((Multiple Authorship))

    DBMol. Cryst. Liq. Cryst.(/)-DB   ( 278 )   247 - 252   1996

  • Three Dimensional Structure of Porcine Kidney D-Amino Acid Oxidase at 3.0 (]E87F8[) Resolution((Multiple Authorship))

    DBJ. Biochem.(/)-DB   ( 120 )   14 - 17   1996

  • Crystallization of Expressed Porcine Kidney D-Amino Acid Oxidase and Preliminary X-Ray Crystallographic Characterization((Multiple Authorship))

    DBJ. Biochem.(/)-DB   ( 199 )   1114 - 1117   1996

  • Two-Molecule Crystals Between Nitroanilines and Nitrophenols((Multiple Authorship))

    DBMol. Cryst. Liq. Cryst.(/)-DB   ( 279 )   265 - 274   1996

  • Solid-state photoaddition reaction in polycrystalline mixtures of NH heteroaromatic compounds and DBtrans-stilbene(/)-DB

    DBJ. Photochem. Photobiol.(/)-DB   85   225 - 229   1995

  • X-Ray Crystallographic Study of Pyridoxamine 5"-phosphate-type Aspartate Aminotransferases from DBEscherichia coli(/)-DB in Three Forms

    DBJ. Biochem.(/)-DB   116   1001 - 1012   1994

  • NMR Studies of D11D1H Resonances in the 10-18-ppm Range for Aspartate Aminotransferase from DBEscherichia coli(/)-DB

    DBJ. Biol. Chem.(/)-DB   269 ( 45 )   28027 - 28033   1994

  • Reaction of Dichlorocarbene with 1-(2,4,6-Tri-t-butylphenyl)-1-phosphaallenes

    DBHeteroatom Chemistry(/)-DB   5 ( 3 )   195 - 200   1994

  • Role of Methanol in Chiral Combination of Host and Guest Molecules in the Inclusion Crystal

    DBJ. Chem. Soc. , Chem. Commun.(/)-DB   1795 - 1796   1994

  • Role of an Active Site Residue Analyzed by Combination of Mutagenesis and Coenzyme Analog

    DBJ. Mol. Biol.(/)-DB   234   1218 - 1229   1993

  • X-ray Analysis of a Captodative Diphosphene 1-(N-Mesityl-9-fluorenylamino)-2-]2,4,6-tris(trifluoromethyl)phenyl[ diphosphene

    DBBull. Chem. Soc. Jpn.(/)-DB   66   3831 - 3833   1993

  • Structure Study of Host-Guest Molecular Association in Solution and in the Solid State

    DBJ. Chem. Soc. , Chem. Commun.(/)-DB   1413 - 1415   1993

  • X-ray analysis of 1-phospha-1,2,3-butatriene as a ligand of carbonyltungusten complex

    DBJ. Organomet. Chem.(/)-DB   461   81 - 84   1993

  • Binuclear Silver(I) Complex with Double Armed Diaza-Crown Ether Containing Short Metal-Metal Separation

    DBChem. Lett.(/)-DB   699 - 703   1992

  • X-ray structure analyses of diphosphinidene cyclobutene and its chelate type tetracarbonylmolybdenum (0) complex

    DBJ. Organomet. Chem.(/)-DB   431   C35-C41   1992

  • The First X-ray Analysis of a Phospha]3[radialene

    DBPolyhedron(/)-DB   11   385 - 387   1992

  • Cheletropic Reactions of Tricyclo]5.3.1.0D14,9D1[ undeca-2, 5-diene with Dichlorophosphines. Synthesis of Novel Phosphapolycycles

    DBChem. Lett.(/)-DB   1677 - 1678   1991

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Presentations

  • エンド-β-N-アセチルグルコサミニダーゼHSにおけるドメインIVの機能 Domestic conference

    倉内郁哉、大倉和貴、田中里佳、細川千絵、伊藤和央、宮原郁子

    2022年量子ビームサイエンスフェスタ  2023.03 

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  • エンド-β-N-アセチルグルコサミニダーゼHSにおけるドメインIVの役割 Domestic conference

    倉内郁哉、大倉和貴、米澤健人、清水伸隆、田中里佳、細川千絵、宮原郁子、伊藤和央

    令和4年(2022年)度日本結晶学会年会  2022.11 

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    Presentation type:Poster presentation  

  • エンド-β-N-アセチルグルコサミニダーゼHSの立体構造とそのドメイン Domestic conference

    倉内郁哉、大倉和貴、米澤健人、清水伸隆、田中里佳、細川千絵、宮原郁子、伊藤和央

    第95回日本生化学会大会  2022.11 

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  • 細菌由来セリンパルミトイル転移酵素における基質認識機構に関する構造学的考察 Domestic conference

    生城浩子 ,村上大毅 ,高橋亜弥 ,堅山あすか、澤井大樹、後藤春菜、サジールクーラス、本田拓巳、村井勇太、門出健次、宮原郁子、神谷信夫、矢野貴人

    第95回日本生化学会大会  2022.11 

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  • 基質が結合した酸素の立体構造から機能を考える Domestic conference

    宮原郁子

    日本ビタミン学会第74回大会  2022.06 

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    Presentation type:Symposium, workshop panel (public)  

  • エンド-β-N-アセチルグルコサミニダーゼHSの立体構造と機能 Domestic conference

    倉内郁哉、大倉和貴、米澤健人、清水伸隆、田中里佳、伊藤和央、細川千絵、宮原郁子

    2022年蛋白質科学会年会  2022.06 

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    Presentation type:Oral presentation (general)  

  • エンド-β-N-アセチルグルコサミニダーゼHSの機能とドメイン構造 Domestic conference

    倉内郁哉、大倉和貴、森 真司、米澤健人、清水伸隆、田中里佳、細川千絵、宮原郁子、伊藤和央

    第22回関西グライコサイエンスフォーラム  2022.05 

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    Presentation type:Oral presentation (general)  

  • エンド-b-N-アセチルグルコサミニダーゼ HSの X線結晶構造解析 Domestic conference

    倉内郁哉、大倉和貴、田中里佳、細川千絵、伊藤和央、宮原郁子

    2021年度量子ビームサイエンスフェスタ  2022.03 

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    Presentation type:Oral presentation (general)  

  • Prevotella melaninogenicaの産生するエンド-β-N-アセチルグルコサミニダーゼPMの立体構造 Domestic conference

    大倉和貴、森 真司、米澤健人、清水伸隆、神谷信夫、宮原郁子、伊藤和央

    日本農芸化学会2022年大会  2022.03 

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    Presentation type:Oral presentation (general)  

  • Endo-β-N-acetylglucosaminidase HSのX線結晶構造解析 Domestic conference

    倉内郁哉、大倉和貴、田中里佳、細川千絵、 伊藤和央、宮原郁子

    令和3年(2021年度)日本結晶学会年会  2021.11 

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  • Prevotella melaninogenicaの産生する宿主糖タンパク質糖鎖遊離酵素の立体構造 Domestic conference

    大倉和貴、森 真司、米澤健人、清水伸隆、神谷信夫、宮原郁子、伊藤和央

    第93回生化学会大会  2021.09 

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  • セリンパルミトイル転移酵素におけるアミノ酸基質の許容性 Domestic conference

    村上大毅 ,生城浩子 ,高橋亜弥 ,矢野貴人 ,神谷信夫,宮原郁子

    ビタミン学会第73回大会  2021.06 

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  • Prevotella melaninogenica の産生する 宿主糖タンパク質糖鎖遊離酵素の立体構造 Domestic conference

    大倉和貴、森 真司、米澤健人、清水伸隆、神谷信夫、宮原郁子、伊藤和央

    第21回関西グライコサイエンスフォーラム  2021.05 

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  • Endo-β-N -acetylglucosaminidase PMαの構造と機能 Domestic conference

    大倉和貴,森真司 ,伊藤和央 ,米澤健人 ,清水伸隆 ,神谷信夫 ,宮原郁子

    2020年度量子ビームサイエンスフェスタ  2021.03 

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  • Endo-β-N-acetylglucosaminidase PMαの立体構造解析 Domestic conference

    大倉和貴 他

    結晶学会70周年記念大会  2020.11 

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  • Prevotella melaninogenicaの産生する宿主糖タンパク質糖鎖遊離酵素の高発現系の構築と立体構造の解析 Domestic conference

    大倉 和貴, 森 真司, 神谷 信夫, 宮原 郁子, 伊藤 和央

    日本生化学会大会プログラム・講演要旨集  2020.09  (公社)日本生化学会

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    Presentation type:Oral presentation (general)  

  • セリンパルミトイル転移酵素の基質特異性 Domestic conference

    村上大毅,生城浩子,高橋亜弥,矢野貴人,神谷信夫,宮原郁子

    日本ビタミン学会第72回大会  2020.06 

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  • 5-アミノレブリン酸合成酵素の基質フリー型の構造解析 Domestic conference

    松永 慶, 生城 浩子, 矢野 貴人, 神谷 信夫, 宮原 郁子

    ビタミン  2020.04  (公社)日本ビタミン学会

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    Presentation type:Oral presentation (general)  

  • Prevotella melaninogenicaの産生する宿主糖タンパク質糖鎖遊離酵素の性質 Domestic conference

    森 真司, 大倉 和貴, 宮原 郁子, 伊藤 和央

    日本生化学会大会プログラム・講演要旨集  2019.09  (公社)日本生化学会

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    Presentation type:Oral presentation (general)  

  • Caulobacter crescentus由来5-アミノレブリン酸合成酵素におけるヘム依存性不活化現象 Domestic conference

    生城 浩子, 長見 篤, 高井 智子, 澤井 大樹, 主馬野 祐希, 堀 洋, 宮原 郁子, 神谷 信夫, 矢野 貴人

    ビタミン  2019.04  (公社)日本ビタミン学会

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    Presentation type:Oral presentation (general)  

  • 細菌由来セリンパルミトイル転移酵素における基質認識機構の解明 Domestic conference

    高橋亜弥,生城浩子,後藤春菜,伊藤恵実,生城真一,平林義雄,矢野貴人,神谷信夫,宮原郁子

    第41回日本分子生物学会年会  2018.11 

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  • 5-アミノレブリン酸合成酵素の基質 酵素三元複合体および生成物 酵素複合体の立体構造解析 Domestic conference

    主馬野 祐希, 生城 浩子, 矢野 貴人, 神谷 信夫, 宮原 郁子

    日本生化学会大会プログラム・講演要旨集  2018.09  (公社)日本生化学会

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  • アミノレブリン酸合成酵素の二元・三元複合体構造に基づく反応制御メカニズム解明 Domestic conference

    主馬野 祐希, 生城 浩子, 矢野 貴人, 神谷 信夫, 宮原 郁子

    ビタミン  2018.04  (公社)日本ビタミン学会

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  • 細菌由来セリンパルミトイル転移酵素における基質アナログ複合体の結晶構造解析 Domestic conference

    高橋 亜弥, 生城 浩子, 後藤 春菜, 伊藤 恵実, 生城 真一, 平林 義雄, 矢野 貴人, 神谷 信夫, 宮原 郁子

    ビタミン  2018.04  (公社)日本ビタミン学会

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  • 反応中間体構造から予測されるセリンヒドロキシメチル基転移酵素の触媒反応機構 Domestic conference

    赤井 翔太, 生城 浩子, 矢野 貴人, 神谷 信夫, 宮原 郁子

    ビタミン  2018.04  (公社)日本ビタミン学会

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    Presentation type:Oral presentation (general)  

  • アシルCoA脱水素酵素における補酵素フラビン-基質アナログ間の相互作用 Domestic conference

    玉置 春彦, 佐藤 恭介, 宮原 郁子

    生命科学系学会合同年次大会  2017.12  生命科学系学会合同年次大会運営事務局

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    Presentation type:Oral presentation (general)  

  • 細菌由来セリンパルミトイル基転移酵素における基質アナログ複合体の結晶構造解析 Domestic conference

    高橋 亜弥, 生城 浩子, 後藤 春菜, 伊藤 恵実, 生城 真一, 平林 義雄, 矢野 貴人, 神谷 信夫, 宮原 郁子

    生命科学系学会合同年次大会  2017.12  生命科学系学会合同年次大会運営事務局

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    Presentation type:Oral presentation (general)  

  • 基質複合体の構造解析によるアミノレブリン酸合成酵素の機能解明 Domestic conference

    主馬野祐希,生城浩子,矢野貴人,神谷信夫,宮原郁子

    結晶学会年会  2017.11 

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    Presentation type:Oral presentation (general)  

  • セリンヒドロキシメチル基転移酵素の反応中間体構造から推定される触媒反応機構 Domestic conference

    赤井翔太,生城浩子,矢野貴人,神谷信夫,宮原郁子

    結晶学会年会  2017.11 

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    Presentation type:Poster presentation  

  • Caulobacter crescentus由来5-アミノレブリン酸合成酵素の生化学的性状解析 Domestic conference

    生城 浩子, 主馬野 祐希, 宮原 郁子, 神谷 信夫, 矢野 貴人

    日本生化学会大会プログラム・講演要旨集  2016.09  (公社)日本生化学会

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    Presentation type:Oral presentation (general)  

  • 長鎖アシルCoA脱水素酵素における補酵素フラビンと基質アナログの相互作用 Domestic conference

    玉置 春彦, 佐藤 恭介, 田中 丈幸, 宮原 郁子

    日本生化学会大会プログラム・講演要旨集  2016.09  (公社)日本生化学会

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    Presentation type:Oral presentation (general)  

  • ホモセリン脱水素酵素・アスパラギン酸-4-セミアルデヒド複合体構造から推定される酵素反応機構 Domestic conference

    水田 啓文, 生城 浩子, 赤井 翔太, 臼杵 克之助, 矢野 貴人, 神谷 信夫, 宮原 郁子

    日本生化学会大会プログラム・講演要旨集  2016.09  (公社)日本生化学会

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    Presentation type:Oral presentation (general)  

  • グリシン複合体の結晶構造が明らかにしたアミノレブリン酸合成酵素における中間体形成の制御 Domestic conference

    主馬野 祐希, 生城 浩子, 矢野 貴人, 神谷 信夫, 宮原 郁子

    ビタミン  2016.04  (公社)日本ビタミン学会

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    Presentation type:Oral presentation (general)  

  • アスパラギン酸アミノトランスフェラーゼとマロン酸の複合体による超閉構造について Domestic conference

    村川 武志, 宮原 郁子, 林 秀行

    ビタミン  2016.01  (公社)日本ビタミン学会

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    Presentation type:Oral presentation (general)  

  • Caulobacter crescentus由来アミノレブリン酸合成酵素の結晶構造解析 Domestic conference

    主馬野 祐希, 生城 浩子, 矢野 貴人, 神谷 信夫, 宮原 郁子

    日本生化学会大会・日本分子生物学会年会合同大会講演要旨集  2015.12  (公社)日本生化学会

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    Presentation type:Oral presentation (general)  

  • アスパラギン酸セミアルデヒド結合型ホモセリン脱水素酵素のX線結晶構造解析 Domestic conference

    赤井 翔太, 水田 啓文, 生城 浩子, 澤井 大樹, 林 秀行, 矢野 貴人, 神谷 信夫, 宮原 郁子

    日本生化学会大会・日本分子生物学会年会合同大会講演要旨集  2015.12  (公社)日本生化学会

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    Presentation type:Oral presentation (general)  

  • アシルCoA脱水素酵素における補酵素フラビンと基質アナログ間の相互作用解析 Domestic conference

    玉置 春彦, 佐藤 恭介, 田中 丈幸, 宮原 郁子

    日本生化学会大会・日本分子生物学会年会合同大会講演要旨集  2015.12  (公社)日本生化学会

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    Presentation type:Oral presentation (general)  

  • セリンヒドロキシメチル基転移酵素のキノノイド中間体の構造 Domestic conference

    主馬野 祐希, 湯川 直樹, 生城 浩子, 林 秀行, 矢野 貴人, 神谷 信夫, 宮原 郁子

    ビタミン  2015.04  (公社)日本ビタミン学会

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    Presentation type:Oral presentation (general)  

  • 高度好熱菌由来ホモセリン脱水素酵素の三元複合体結晶構造解析 Domestic conference

    赤井 翔太, 生城 浩子, 澤井 大樹, 林 秀行, 矢野 貴人, 神谷 信夫, 宮原 郁子

    ビタミン  2014.04  (公社)日本ビタミン学会

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    Presentation type:Oral presentation (general)  

  • 耐熱性スフィンゴシン1-リン酸リアーゼの生化学的解析 Domestic conference

    生城 浩子, 青木 雅彦, 東城 博雅, 湯川 直樹, 宮原 郁子, 神谷 信夫, 林 秀行

    日本生化学会大会プログラム・講演要旨集  2012.12  (公社)日本生化学会

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    Presentation type:Oral presentation (general)  

  • セリンヒドロキシメチル基転移酵素の基質認識 Domestic conference

    湯川 直樹, 生城 浩子, 後藤 勝, 林 秀行, 神谷 信夫, 宮原 郁子

    日本生化学会大会プログラム・講演要旨集  2011.09  (公社)日本生化学会

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    Presentation type:Oral presentation (general)  

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Grant-in-Aid for Scientific Research

  • Molecular mechanism of the sphingolipid-like mycotoxin production in the pathogenic fungi

    Grant-in-Aid for Scientific Research(C)  2013.04

  • Fundamental Research for the synthesis of biorecognition glycosides by enzymatic introduction of oligosaccharides from glycoproteins

    Grant-in-Aid for Scientific Research(C)  2009

  • Structure and function of membrane-bound quinohemoprotein Alcohol dehydrogenase

    Grant-in-Aid for Scientific Research(C)  2007

  • フェムト秒パルスの自己収束効果が誘起する蛋白質の結晶核の生成

    萌芽研究  2006

  • Four-dimensional structure analysis of Ndx family enzyme utilizing a new pH-temperature jump trigger

    Grant-in-Aid for Scientific Research(B)  2006

  • Structure and function of PLP-dependent enzyme and its homolog involved in higher brain functions

    Grant-in-Aid for Scientific Research(B)  2004

  • X-ray Crystallographic Studies on Mechanism of Photosystem II Membrane Protein Complex

    Grant-in-Aid for Scientific Research on Priority Areas  2004

  • アルギニン合成系タンパク質のX線構造解析

    Grant-in-Aid for Young Scientists(B)  2002

  • ヒスチジン合成系タンパク質のX線結晶解析

    Grant-in-Aid for Scientific Research on Priority Areas  2001

  • Precise structure and double substrate recognition of pyridoxal protein

    Grant-in-Aid for Scientific Research(B)  2001

  • High Resolution X-ray Study of Cofactor Dependent Enzyme and Biocatalyst Design

    Grant-in-Aid for Scientific Research on Priority Areas  2001

  • アミノ基転移酵素における基質認識機構の多様性と類似性

    奨励研究(A)  2000

  • フラビン酵素の高次構造と電子移動反応

    特定領域研究(A)  2000

  • フラビン酵素の高次構造と電子移動反応

    特定領域研究(A)  1999

  • フラビン酵素の高次構造と電子移動反応

    特定領域研究(A)  1998

  • 高品質結晶によるアミノ基転移酵素の反応中間体の高分解能構造解析

    Grant-in-Aid for Scientific Research on Priority Areas  1996

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Outline of education staff

  • 大学1年次理系学生のための化学実験の基礎的な指導、および理学部化学科3年次のための固体化学の講義および物理化学実験においてX線回折法をもちいた構造解析の実習教育。

Charge of on-campus class subject

  • 基礎化学実験

    2022   Weekly class   Undergraduate

  • 初年次ゼミナール

    2022   Weekly class   Undergraduate

  • 研究企画ゼミナール

    2022   Intensive lecture   Graduate school

  • 物理化学特論B

    2022   Weekly class   Graduate school

  • 理科基礎セミナー

    2022   Intensive lecture   Undergraduate

  • 生化学1

    2022   Weekly class   Undergraduate

  • 化学前期特別研究(M2)

    2022   Intensive lecture   Graduate school

  • 機能分子科学演習(M2)

    2022   Intensive lecture   Graduate school

  • 固体化学

    2022   Weekly class   Undergraduate

  • 特別研究(化学)

    2022   Intensive lecture   Undergraduate

  • 化学概論

    2022   Weekly class   Undergraduate

  • 化学実験II

    2021     Undergraduate

  • 基礎物理化学A

    2021     Undergraduate

  • 基礎化学実験1

    2021     Undergraduate

  • 後期特別研究

    2021     Graduate school

  • 前期特別研究

    2021     Graduate school

  • 固体化学

    2021     Undergraduate

  • 化学概論

    2021     Undergraduate

  • 生化学1

    2021     Undergraduate

  • 機能分子科学演習

    2021     Graduate school

  • 理科基礎セミナー

    2021     Undergraduate

  • 機能先端分子科学特論

    2021     Graduate school

  • 基礎物理化学A

    2020     Undergraduate

  • 基礎化学実験1

    2020     Undergraduate

  • 後期特別研究

    2020     Graduate school

  • 前期特別研究

    2020     Graduate school

  • 化学概論

    2020     Undergraduate

  • 固体化学

    2020     Undergraduate

  • 生化学1

    2020     Undergraduate

  • 機能分子科学演習

    2020     Graduate school

  • 理科基礎セミナー

    2020     Undergraduate

  • 化学実験II

    2020     Undergraduate

  • 基礎物理化学A

    2019     Undergraduate

  • 機能先端分子科学特論

    2019     Graduate school

  • 理科基礎セミナー

    2019     Undergraduate

  • 機能分子科学演習

    2019     Graduate school

  • 生化学1

    2019     Undergraduate

  • 固体化学

    2019     Undergraduate

  • 化学概論

    2019     Undergraduate

  • 前期特別研究

    2019     Graduate school

  • 後期特別研究

    2019     Graduate school

  • 基礎化学実験1

    2019     Undergraduate

  • 化学実験II

    2019     Undergraduate

  • 理科基礎セミナー

    2018     Undergraduate

  • 後期特別研究

    2018     Graduate school

  • 前期特別研究

    2018     Graduate school

  • 機能分子科学演習

    2018     Graduate school

  • 化学概論

    2018     Undergraduate

  • 固体化学

    2018     Undergraduate

  • 化学実験II

    2018     Undergraduate

  • 生化学1

    2018     Undergraduate

  • 基礎化学実験1

    2018     Undergraduate

  • 後期特別研究

    2017     Graduate school

  • 前期特別研究

    2017     Graduate school

  • 機能分子科学演習

    2017     Graduate school

  • 機能分子物理化学特論I

    2017     Graduate school

  • 化学概論

    2017     Undergraduate

  • 化学実験II

    2017     Undergraduate

  • 生化学1

    2017     Undergraduate

  • 基礎化学実験1

    2017     Undergraduate

  • 基礎物理化学A

    2017     Undergraduate

  • 分子相関化学演習

    1900    

  • 基礎化学実験1

    1900     Undergraduate

  • 基礎物理化学A

    1900     Undergraduate

  • 後期特別研究

    1900    

  • 分子相関ゼミナール

    1900    

  • 前期特別研究

    1900    

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Social Activities

  • 結晶への探検

    Role(s): Logistic support

    2019.11

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    Audience: Schoolchildren, Junior high school students, High school students, Parents/Guardians, General public

    Type:Cooperation with government and educational institutions

  • 結晶への探検

    Role(s): Host, Logistic support

    2018.11

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    Audience: Schoolchildren, Junior high school students, High school students, Parents/Guardians, General public

    Type:Cooperation with government and educational institutions

Foreigner acceptance

  • 2019

    foreigners accepted :0

  • 2018

    foreigners accepted :0

  • 2017

    foreigners accepted :0

Other

  • Job Career

    2006

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    Osaka City University

  • Job Career

    1998

      More details

    Osaka City University

  • Job Career

    1994

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    Osaka City University