Updated on 2024/04/02

写真a

 
TSUBOUCHI Taishi
 
Organization
Graduate School of Medicine Department of Basic Medical Science Associate Professor
School of Medicine Department of Medical Science
Title
Associate Professor
Affiliation
Institute of Medicine
Homepage
http://www.med.osaka-cu.ac.jp/bacteriology/
Affiliation campus
Abeno Campus,　Other campuses

Position

  • Graduate School of Medicine Department of Basic Medical Science 

    Associate Professor  2022.04 - Now

  • School of Medicine Department of Medical Science 

    Associate Professor  2022.04 - Now

Degree

  • 博士（農学） （ The University of Tokyo ）

Research Areas

  • Life Science / Pharmaceutical chemistry and drug development sciences  / Pharmaceuticals - chemistry and drug development

  • Life Science / Bacteriology  / Bacteriology

  • Life Science / Applied microbiology  / Applied microbiology

Research Interests

  • Deepsea microbes/Marine microbes（Actinomycetales）

  • Anticancer agent

  • Bioinfomatics/Omics

  • Antimicrobial agent/Antiirus agent

  • Mechanism of antimicrobial resistance

  • Natural products chemistry

  • Drug discovery

  • 転写制御機構

  • Antimicrobial resistance

Research subject summary

  • 「海洋（深海）性微生物の培養株化とその有効活用」
    1. 新奇海洋性微生物の効率的な分離、およびその利活用
    2. 海洋性放線菌が生産する新奇抗多剤耐性菌物質の構造解析、および特性解析
    3. 海洋性微生物が生産する新奇抗ウイルス物質の構造解析、および特性解析
    4. 超多剤耐性菌の薬剤耐性メカニズムの解明

Professional Memberships

  • The Japanese Association for Infectious Disease

    2019.01 - Now

  • SOCIETY OF ACTINOMYCETES JAPAN

    2016.06 - Now

  • The Japanese Society for Marine Biotechnology

    2013.03 - Now

  • Japan Society for Bioscience, Biotechnology, and Agrochemistry

    2002.04 - Now

Committee Memberships (off-campus)

  • 遺伝子組換え実験安全委員会委員   大阪電気通信大学  

    2023.04 - 2024.03 

  • Member of the Safety Committee for Genetic Recombination Experiments   Osaka Electro-Communication University  

    2021.04 - Now 

  • 若手の会コア委員   マリンバイオテクノロジー学会  

    2014.06 - Now 

Job Career (off-campus)

  • Osaka Metropolitan University   Graduate School of Medicine Basic Medicine Course

    2022.04 - Now

  • Tokyo University of Agriculture   Faculty of Life Sciences / Department of Bioscience   Part-time lecturer

    2017.12 - Now

  • 熊本大学   薬学部   非常勤講師

    2017.12 - 2018.11

  • Japan Agency for Marine-earth Science and Technology   Research and Development (R&D) Center for Marine Biosciences   Vice Senior Staff Member of Technology

    2013.04 - 2017.06

  • Japan Agency for Marine-earth Science and Technology   Scientist

    2008.04 - 2013.03

  • RIKEN   Genome Sciences Research Complex   Research associate

    2005.03 - 2007.06

▼display all

Education

  • The University of Tokyo   Graduate School of Agricultural and Life Sciences   Doctor's Course   Graduated/Completed

    1999.04 - 2005.03

  • Nihon University     Graduated/Completed

    1995.04 - 1999.03

Papers

  • Haneummycin, a new 22-membered macrolide lactam antibiotic, produced by marine-derived Streptomyces sp. KM77-8.

    Uemura M, Kobayashi K, Sato N, Nagai K, Seki R, Kamio M, Fukuda T, Tsubouchi T, Tomoda H, Ohshiro T, Kobayashi T, Terahara T

    The Journal of antibiotics   76 ( 11 )   650 - 657   2023.11（ ISSN:0021-8820 ）

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  • Rifampicin exerts anti-mucoviscous activity against hypervirulent Klebsiella pneumoniae via binding to the RNA polymerase β subunit.

    Mitsunori Tohda, Ken-Ichi Oinuma, Arata Sakiyama, Taishi Tsubouchi, Mamiko Niki, Hiroki Namikawa, Kenshi Yamane, Koichi Yamada, Tetsuya Watanabe, Kazuhisa Asai, Hiroshi Kakeya, Yukihiro Kaneko, Tomoya Kawaguchi

    Journal of global antimicrobial resistance   32   21 - 28   2023.03（ ISSN:2213-7165 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    OBJECTIVES: In hypervirulent Klebsiella pneumoniae (hvKP), the hypermucoviscous capsule is known to be a major virulence determinant. We previously discovered that rifampicin (RFP), a bactericidal drug that binds to and inhibits the β subunit of RNA polymerase (RpoB), elicits anti-mucoviscous activity against hvKP by suppressing rmpA, a regulator of capsule production. Here, we aimed to determine whether RFP exerts this effect at sub-growth-inhibitory concentrations via its binding to RpoB. METHODS: Five spontaneous RFP-resistant mutants (R1-R5) were prepared from an hvKP clinical isolate and subjected to whole genome sequencing and mucoviscosity analyses. Subsequently, a two-step allelic exchange procedure was used to create a rpoB mutant R6 and revertants with wild-type rpoB from R1-R5 (named R1'-R5'). Transcription levels of rmpA and the capsular polysaccharide polymerase gene magA and capsule thickness of R1-R5 and R1'-R5' grown without or with RFP were evaluated by quantitative reverse transcription polymerase chain reaction and microscopic observation using India ink staining. RESULTS: R1-R5 all had non-synonymous point mutations in rpoB and were highly resistant to the bactericidal effects and anti-mucoviscous activity of RFP. While the properties of R6 were similar to those of R1-R5, the responses of R1'-R5' to RFP were identical to those of the wild type. rmpA and magA transcription levels and capsule thickness correlated well with the mucoviscosity levels. CONCLUSIONS: RFP exerts anti-mucoviscous activity by binding to RpoB. The mechanism of how this causes rmpA suppression remains to be explored.

    DOI： 10.1016/j.jgar.2022.11.018

    PubMed

  • A Culture Supernatant from an Actinomycete sp. Affects Biofilm Formation and Virulence Expression of <i>Candida auris</i> Reviewed

    Yamane Kenshi, Niki Mamiko, Tsubouchi Taishi, Watanabe Tetsuya, Asai Kazuhisa, Oinuma Ken-Ichi, Sakiyama Arata, Saren Chaogetu, Matsumoto Yuki, Makimura Koichi, Kaneko Yukihiro, Kawaguchi Tomoya

    Medical Mycology Journal   64 ( 1 )   7 - 17   2023.02（ ISSN:21856486 ） （ eISSN:2186165X ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：Domestic journal  

    The multidrug-resistant pathogen <i>Candida auris</i> is characterized by its aggregation under certain conditions, which affects its biofilm formation, drug susceptibility, and pathogenicity. Although the innate tendency to aggregate depends on the strain, the mechanism regulating <i>C. auris</i> aggregation remains unclear. We found that the culture supernatant from one of the 95 <i>Actinomyces</i> strains isolated from a deep-sea environment (IMAs2016D-66) inhibited <i>C. auris</i> aggregation. The cells grown in the presence of IMAs2016D-66 exhibited reduced hydrophobicity, biofilm formation, and enhanced proteolytic activity. In addition, the efflux pump activity of the fluconazole-resistant <i>C. auris</i> strain LSEM 3673 was stimulated by IMAs2016D-66, whereas no significant change was observed in the fluconazole-susceptible strain LSEM 0643. As the relationship between aggregative tendency and virulence in <i>C. auris</i> is still unclear, IMAs2016D-66 can serve as a tool for investigating regulatory mechanisms of phenotype switching and virulence expression of <i>C. auris</i>. Understanding of phenotype switching may help us not only to understand the pathogenicity of <i>C. auris</i>, but also to design new drugs that target the molecules regulating virulence factors.

    DOI： 10.3314/mmj.22-00026

    PubMed

  • Siderophore production as a biomarker for Klebsiella pneumoniae strains that cause sepsis: A pilot study.

    Hiroki Namikawa, Makoto Niki, Mamiko Niki, Ken-Ichi Oinuma, Koichi Yamada, Kiyotaka Nakaie, Taishi Tsubouchi, Yoshihiro Tochino, Yasuhiko Takemoto, Yukihiro Kaneko, Hiroshi Kakeya, Taichi Shuto

    Journal of the Formosan Medical Association = Taiwan yi zhi   121 ( 4 )   848 - 855   2022.04（ ISSN:0929-6646 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    BACKGROUND/PURPOSE: Klebsiella pneumoniae bacteremia-induced sepsis is a clinically important condition with a high mortality rate and various known virulence factors. However, studies on the association of these virulence factors with the occurrence of K. pneumoniae bacteremia-induced sepsis are scarce. We aimed to investigate clinical variables and virulence factors in patients with K. pneumoniae bacteremia-induced sepsis. METHODS: We retrospectively reviewed the medical records of 76 patients with K. pneumoniae bacteremia between January 2012 and July 2017. Patients were divided into sepsis (n = 25) and non-sepsis (n = 51) groups. Patient background characteristics, antimicrobial regimens, and prognosis were evaluated. We assessed the distribution of virulence factors related to K. pneumoniae, such as mucoviscosity, capsular polysaccharide, and siderophores. Siderophore production levels were determined by measuring the orange halo zone on chrome azurol S agar plate assay. RESULTS: There were no intergroup differences in male-to-female ratio and age. Multivariable analysis revealed that siderophore production level (p < 0.01) was an independent predictor of K. pneumoniae bacteremia-induced sepsis. Furthermore, the optimal cut-off point of siderophore production to predict sepsis was 9.6 mm (sensitivity, 86%; specificity, 76%; AUC, 0.81). CONCLUSION: Siderophore production was an independent predictor of sepsis caused by K. pneumoniae bacteremia. The optimal cut-off point for siderophore production for sepsis occurrence prediction was 9.6 mm. To improve outcomes, patients with K. pneumoniae bacteremia-induced sepsis with high siderophore production levels should be managed prudently.

    DOI： 10.1016/j.jfma.2021.06.027

    PubMed

  • Genomic characterization of triple-carbapenemase-producing Acinetobacter baumannii.

    Ken-Ichi Oinuma, Masato Suzuki, Arata Sakiyama, Taishi Tsubouchi, Kozo Saeki, Kanako Sato, Mamiko Niki, Koichi Yamada, Keigo Shibayama, Hiroshi Kakeya, Yukihiro Kaneko

    JAC-antimicrobial resistance   3 ( 4 )   dlab191   2021.12

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Objectives: To characterize Acinetobacter baumannii OCU_Ac16a, a clinical isolate co-harbouring three acquired carbapenemase genes, bla NDM-1, bla TMB-1, and bla OXA-58, and assess the clinical significance of so-called multiple-carbapenemase producers. Methods: OCU_Ac16a and its close relative, OCU_Ac16b, which lacks the bla NDM-1, were isolated from sputum cultures of a patient at Osaka City University Hospital. We subjected these strains to whole-genome analysis, particularly focusing on the genetic context of each carbapenemase gene. The transmissibility and functionality of each carbapenemase gene were analysed by conjugation and transformation experiments and antimicrobial susceptibility tests. Results: bla TMB-1 was located in a class 1 integron on the chromosome, whereas bla NDM-1 and bla OXA-58 were found on plasmids named pOCU_Ac16a_2 and pOCU_Ac16a_3, respectively. pOCU_Ac16a_2 (which exhibited highly efficient self-transmissibility) and pOCU_Ac16a_3 (which did not show transmissibility but could be introduced into another A. baumannii strain via electroporation) could both confer carbapenem resistance (MICs ≥512 and ≥32 mg/L, respectively) on the recipient strain. The functionality of bla TMB-1 was evident from the high resistance of OCU_Ac16b to ceftazidime and cefepime (MICs ≥256 and 48 mg/L, respectively), and the high resistance of OCU_Ac16a to cefiderocol (MIC 32 mg/L) could be explained by the additive effect of bla NDM-1 and bla TMB-1. Conclusions: Our data revealed the genomic organization of OCU_Ac16a and demonstrated that all the carbapenemase genes are functional, each contributing to the extremely high broad-spectrum resistance of OCU_Ac16a to β-lactams. As multiple-carbapenemase producers can be serious health threats as drug-resistant pathogens and disseminators of carbapenemase genes, close attention should be paid to their emergence.

    DOI： 10.1093/jacamr/dlab191

    PubMed

  • Complete Genome Sequence of a Clinical Isolate of Acinetobacter baumannii Harboring 11 Plasmids.

    Arata Sakiyama, Yuki Matsumoto, Taishi Tsubouchi, Masato Suzuki, Makoto Niki, Mamiko Niki, Ken-Ich Oinuma, Hiroshi Kakeya, Yukihiro Kaneko

    Microbiology resource announcements   10 ( 39 )   e0069521   2021.09

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Here, we report the complete genome sequence of an Acinetobacter baumannii isolate harboring 11 plasmids, obtained at a hospital in Japan in 2016. The complete 4.07-Mbp genome sequence (1 chromosome and 11 plasmids) was analyzed by a combination of long-read (Flongle) and short-read (NovaSeq 6000) sequencing.

    DOI： 10.1128/MRA.00695-21

    PubMed

  • Complete Genome Sequence of Acinetobacter pittii OCU_Ac17, Isolated from Human Venous Blood.

    Yuki Matsumoto, Arata Sakiyama, Taishi Tsubouchi, Masato Suzuki, Makoto Niki, Mamiko Niki, Hiroshi Kakeya, Yukihiro Kaneko

    Microbiology resource announcements   10 ( 39 )   e0069621   2021.09

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Acinetobacter pittii isolate OCU_Ac17 was obtained from the venous blood of a patient at a hospital in Japan. We present its complete 4.108-Mbp genome sequence (1 chromosome plus 3 plasmids), analyzed by combining long-read (Flongle) and short-read (MiniSeq) sequencing.

    DOI： 10.1128/MRA.00696-21

    PubMed

  • Complete Genome Sequence of Polycladomyces abyssicola JIR-001T, Isolated from Hemipelagic Sediment in Deep Seawater. Reviewed

    Taishi Tsubouchi, Yukihiro Kaneko

    Microbiology resource announcements   10 ( 24 )   e0043521   2021.06

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    Authorship：Lead author,　Corresponding author   Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Here, we report the complete genome sequence of Polycladomyces abyssicola strain JIR-001, which we isolated from hemipelagic sediment in deep seawater. The genome, generated by combining long-read (Flongle) and short-read (NovaSeq) sequencing data, is 3,197,230 bp, with a mean G+C content of 52.0%.

    DOI： 10.1128/MRA.00435-21

    PubMed

  • Cryptic fungal diversity revealed in deep-sea sediments associated with whale-fall chemosynthetic ecosystems. Reviewed

    Nagano Y, Miura T, Tsubouchi T, Lima AO, Kawato M, Fujiwara Y, Fujikura K

    Mycology   11 ( 3 )   263 - 278   2020.08（ ISSN:2150-1203 ）

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    Publishing type：Research paper (scientific journal)  

    In this study, sediments from whale-fall chemosynthetic ecosystems (two different sites, one naturally occurring at 4200 m water depth in South Atlantic Ocean and one artificially immersed at 100 m water depth in Kagoshima Bay, Japan) were investigated by Ion Torrent PGM sequencing of the ITS region of ribosomal RNA to reveal fungal communities in these unique marine environments. As a result, a total of 107 (897 including singletons) Operational Taxonomic Units (OTUs) were obtained from the samples explored. Composition of the 107 OTUs at the phylum level among the five samples from two different whale-fall sites was assigned to Ascomycota (46%), Basidiomycota (7%), unidentified fungi (21%), non-fungi (10%), and sequences with no affiliation to any organisms in the public database (No-match) (16%). The high detection of the unidentified fungi and unassigned fungi was revealed in the whale-fall environments in this study. Some of these unidentified fungi are allied to early diverging fungi and they were more abundant in the sediments not directly in contact with whalebone. This study suggests that a cryptic fungal community exists in unique whale-fall ecosystems.

    DOI： 10.1080/21501203.2020.1799879

    PubMed

  • Nutritional status positively impacts humoral immunity against its Mycobacterium tuberculosis, disease progression, and vaccine development Reviewed

    Niki Mamikoc, Yoshiyama Takashi, Nagai Hideaki, Miyamoto Yuji, Niki Makoto, Oinuma Ken-ichi, Tsubouchi Taishi, Kaneko Yukihiro, Matsumoto Sohkichi, Sasaki Yuka, Hoshino Yoshihiko

    PLOS ONE   15 ( 8 )   e0237062   2020.08（ ISSN:1932-6203 ）

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    Publishing type：Research paper (scientific journal)  

    Nutritional status contributes to the regulation of immune responses against pathogens, and malnutrition has been considered as a risk factor for tuberculosis (TB). Mycobacterium tuberculosis (Mtb), the causative agent of TB, can modulate host lipid metabolism and induce lipid accumulation in macrophages, where the bacilli adopt a dormant phenotype. In addition, serum lipid components play dual roles in the regulation of and protection from Mtb infection. We analyzed the relationship between nutritional status and the humoral immune response in TB patients. We found that serum HDL levels are positively correlated with the serum IgA specific for Mtb antigens. Analysis of the relationship between serum nutritional parameters and clinical parameters in TB patients showed that serum albumin and CRP levels were negatively correlated before treatment. We also observed reduced serum LDL levels in TB patients following treatment. These findings may provide insight into the role of serum lipids in host immune responses against Mtb infection. Furthermore, improving the nutritional status may enhance vaccination efficacy.

    DOI： 10.1371/journal.pone.0237062

    PubMed

  • Complete Genome Sequence of Acinetobacter baumannii ATCC 19606(T), a Model Strain of Pathogenic Bacteria Causing Nosocomial Infection Reviewed

    Tsubouchi Taishi, Suzuki Masato, Niki Makoto, Oinuma Ken-Ichi, Niki Mamiko, Kakeya Hiroshi, Kaneko Yukihiro

    MICROBIOLOGY RESOURCE ANNOUNCEMENTS   9 ( 20 )   2020.05（ ISSN:2576-098X ）

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    Publishing type：Research paper (scientific journal)  

    Acinetobacter baumannii ATCC 19606T, which is often used in genetic studies as a routine model microorganism, belongs to sequence type 52 (ST52), showing beta-lactam resistance. We present the complete 3.996-Mbp genome sequence (1 chromosome plus 2 plasmids), generated by combining long-read (MinION) and short-read (MiniSeq) sequencing data.

    DOI： 10.1128/MRA.00289-20

    PubMed

  • Clinical and virulence factors related to the 30-day mortality of Klebsiella pneumoniae bacteremia at a tertiary hospital: a case-control study Reviewed

    Namikawa Hiroki, Niki Makoto, Niki Mamiko, Yamada Koichi, Nakaie Kiyotaka, Sakiyama Arata, Oinuma Ken-Ichi, Tsubouchi Taishi, Tochino Yoshihiro, Takemoto Yasuhiko, Kaneko Yukihiro, Shuto Taichi, Kakeya Hiroshi

    EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES   38 ( 12 )   2291 - 2297   2019.12（ ISSN:0934-9723 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s10096-019-03676-y

    PubMed

  • Clinical characteristics of bacteremia caused by hypermucoviscous Klebsiella pneumoniae at a tertiary hospital Reviewed

    Namikawa Hiroki, Yamada Koichi, Sakiyama Arata, Imoto Waki, Yamairi Kazushi, Shibata Wataru, Yoshii Naoko, Niki Makoto, Nakaie Kiyotaka, Oinuma Ken-Ichi, Tsubouchi Taishi, Niki Mamiko, Tochino Yoshihiro, Takemoto Yasuhiko, Kaneko Yukihiro, Shuto Taichi, Kakeya Hiroshi

    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE   95 ( 1 )   84 - 88   2019.09（ ISSN:0732-8893 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.diagmicrobio.2019.04.008

    PubMed

  • Discovery of anti-mucoviscous activity of rifampicin and its potential as a candidate antivirulence agent against hypervirulent Klebsiella pneumoniae Reviewed

    Namikawa Hiroki, Oinuma Ken-Ichi, Sakiyama Arata, Tsubouchi Taishi, Tahara Yuhei O., Yamada Koichi, Niki Mamiko, Takemoto Yasuhiko, Miyata Makoto, Kaneko Yukihiro, Shuto Taichi, Kakeya Hiroshi

    INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS   54 ( 2 )   167 - 175   2019.08（ ISSN:0924-8579 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ijantimicag.2019.05.018

    PubMed

  • Special feature 何が同じで,どこが違う?　現場向けESBL産生菌･CRE(CPE)対策 ■Important　ESBL産生菌･CRE(CPE)対策のための3大知見-両耐性菌の共通･相違の視点から ❷定義と分類の最新知見

    佐伯 康匠, 坪内 泰志, 金子 幸弘

    感染対策ICTジャーナル   14 ( 3 )   189 - 194   2019.07（ ISSN:18814964 ）

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  • Molecular and epidemiological analysis of IMP-1 metallo-beta-lactamase-producing Klebsiella pneumoniae in a tertiary care hospital in Japan Reviewed

    Yamakawa Hiromi, Kosai Kosuke, Akamatsu Norihiko, Matsuda Junichi, Kaku Norihito, Uno Naoki, Morinaga Yoshitomo, Hasegawa Hiroo, Tsubouchi Taishi, Kaneko Yukihiro, Miyazaki Taiga, Izumikawa Koichi, Mukae Hiroshi, Yanagihara Katsunori

    JOURNAL OF INFECTION AND CHEMOTHERAPY   25 ( 4 )   240 - 246   2019.04（ ISSN:1341-321X ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jiac.2018.11.012

    PubMed

  • 日本の三次医療機関におけるIMP-1メタロ-β-ラクタマーゼ産生肺炎桿菌の分子的・疫学的解析(Molecular and epidemiological analysis of IMP-1 metallo-β-lactamase-producing Klebsiella pneumoniae in a tertiary care hospital in Japan) Reviewed

    Yamakawa Hiromi, Kosai Kosuke, Akamatsu Norihiko, Matsuda Junichi, Kaku Norihito, Uno Naoki, Morinaga Yoshitomo, Hasegawa Hiroo, Tsubouchi Taishi, Kaneko Yukihiro, Miyazaki Taiga, Izumikawa Koichi, Mukae Hiroshi, Yanagihara Katsunori

    エルゼビア・ジャパン(株)　Journal of Infection and Chemotherapy   25 ( 3-4 )   240 - 246   2019.04（ ISSN:1341-321X ）

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    Publishing type：Research paper (scientific journal)  

    当院で2009年〜2015年の間に単離された肺炎桿菌を対象として、そのカルバペネマーゼ産生菌(CPS)の分子的・表現型特性を評価し、CPS発現の危険因子を検討した。この検討ではカルバペネマーゼ遺伝子の存在とプラスミド特性を評価し、multilocus sequence typingを実施してCPSに対する電気泳動法(PFGE)の結果に基づいた樹形図を作成し、患者の症例対照研究も行った。その結果、本学保存菌株で、イミペネム(IPM)とメロペネム(MEPM)に1μg/mL以上のMIC値を示す肺炎桿菌88株のうち、18株がIMP型カルバペネマーゼ遺伝子を持ち、これらは7種類の配列型(ST)に分類され、ST1471が最も一般的であった。樹形図では一部の菌株にPFGEパターン、STおよびCPS単離病棟の関連性に顕著な類似性を認め、プラスミド不和合性はIMP-1産生株間で類似していた。また、IMP-1産生株獲得の危険因子は肝疾患と過去のカルバペネム・抗真菌薬の使用であった。

  • Complete Genome Sequence of Sphingobium sp. Strain YG1, a Lignin Model Dimer- Metabolizing Bacterium Isolated from Sediment in Kagoshima Bay, Japan Reviewed

    Ohta Yukari, Shimane Yasuhiro, Nishi Shinro, Ichikawa Junko, Kurosawa Kanako, Tsubouchi Taishi, Ishii Shun'ichi

    MICROBIOLOGY RESOURCE ANNOUNCEMENTS   6 ( 17 )   2018.04（ ISSN:2576-098X ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1128/genomeA.00267-18

    PubMed

  • Draft Genome Sequence of the Arsenic-Resistant Bacterium Brevundimonas denitrificans TAR-002(T) Reviewed

    Tsubouchi Taishi, Kaneko Yukihiro

    MICROBIOLOGY RESOURCE ANNOUNCEMENTS   5 ( 47 )   2017.11（ ISSN:2576-098X ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1128/genomeA.01326-17

    PubMed

  • Electrical Retrieval of Living Streptomycete Spores Using a Potential-Controlled ITO Electrode Reviewed

    Sumihiro Koyama, Shinro Nishi, Yuriko Nagano, Akihiro Tame, Katsuyuki Uematsu, Yuichi Nogi, Yuji Hatada, Taishi Tsubouchi

    ELECTROCHEMICAL SOC JAPAN　ELECTROCHEMISTRY   85 ( 6 )   297 - 309   2017.06（ ISSN:1344-3542 ）

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    Publishing type：Research paper (scientific journal)  

    The purpose of this study was to develop a novel electrical retrieval method for living spores of streptomycetes. Five strains of deep-sea Streptomyces sp. and typical strain Streptomyces albus suspended in 1/20 artificial seawater (1/20ASW) were selectively attached to an indium tin oxide/glass (ITO) working electrode region to which a -0.2 V vs. Ag/AgCI constant potential was applied for 24 h. Spores of all six streptomycetes produced either short fibrous or membranous materials and attached to the ITO electrode region. A +/- 20 mV vs. Ag/AgCI, 12 MHz sine-wave potential was further applied for another 1 h to the deep-sea Streptomyces sp. ST.28 spores on the ITO electrode and colony-forming units increased 3.5 fold after 3 day cultivation compared with controls. The ST.28 spores almost completely lost the ability to adhere to the potential -applied ITO electrode when dispersed in Mg2+ free 1/20ASW. We succeeded in the attachment and cultivation of specifically positioned single ST.28 spores on an ITO microelectrode surface with -0.2 V vs. Ag/AgCI potential application. A combination of micropatterning techniques for a living single streptomycete spore on the ITO electrode and omics technologies holds potential for new bioactive compound screening concepts and applications. (C) The Electrochemical Society of Japan, All rights reserved.

    DOI： 10.5796/electrochemistry.85.297

  • Halarchaeum grantii sp. nov., a moderately acidophilic haloarchaeon isolated from a commercial salt sample. Reviewed

    Shimane Y, Minegishi H, Echigo A, Kamekura M, Itoh T, Ohkuma M, Tsubouchi T, Usui K, Maruyama T, Usami R, Hatada Y

    International journal of systematic and evolutionary microbiology   65 ( 11 )   3830 - 3835   2015.11（ ISSN:1466-5026 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1099/ijsem.0.000501

    PubMed

  • Electrical Retrieval of Living Microorganisms from Cryopreserved Marine Sponges Using a Potential-Controlled Electrode. Reviewed

    Koyama S, Nishi S, Tokuda M, Uemura M, Ishikawa Y, Seya T, Chow S, Ise Y, Hatada Y, Fujiwara Y, Tsubouchi T

    Marine biotechnology (New York, N.Y.)   17 ( 5 )   678 - 92   2015.10（ ISSN:1436-2228 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s10126-015-9651-y

    PubMed

  • Involvement of flocculin in negative potential-applied ITO electrode adhesion of yeast cells. Reviewed

    Koyama S, Tsubouchi T, Usui K, Uematsu K, Tame A, Nogi Y, Ohta Y, Hatada Y, Kato C, Miwa T, Toyofuku T, Nagahama T, Konishi M, Nagano Y, Abe F

    FEMS yeast research   15 ( 6 )   2015.09（ ISSN:1567-1356 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/femsyr/fov064

    PubMed

  • Aneurinibacillus tyrosinisolvens sp. nov., a tyrosine-dissolving bacterium isolated from organics- and methane-rich seafloor sediment. Reviewed

    Tsubouchi T, Mori K, Miyamoto N, Fujiwara Y, Kawato M, Shimane Y, Usui K, Tokuda M, Uemura M, Tame A, Uematsu K, Maruyama T, Hatada Y

    International journal of systematic and evolutionary microbiology   65 ( Pt 6 )   1999 - 2005   2015.06（ ISSN:1466-5026 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1099/ijs.0.000213

    PubMed

  • Draft Genome Sequence of Aneurinibacillus tyrosinisolvens LL-002T, Which Possesses Some Pseudouridine Synthases. Reviewed

    Tsubouchi T, Nishi S, Maruyama T, Hatada Y

    Genome announcements   3 ( 3 )   2015.05

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1128/genomeA.00529-15

    PubMed

  • Draft Genome Sequence of Novosphingobium sp. Strain MBES04, Isolated from Sunken Wood from Suruga Bay, Japan. Reviewed

    Ohta Y, Nishi S, Kobayashi K, Tsubouchi T, Iida K, Tanizaki A, Kurosawa K, Adachi A, Nishihara M, Sato R, Hasegawa R, Hatada Y

    Genome announcements   3 ( 1 )   2015.01

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1128/genomeA.01373-14

    PubMed

  • Brevundimonas denitrificans sp. nov., a denitrifying bacterium isolated from deep subseafloor sediment. Reviewed

    Tsubouchi T, Koyama S, Mori K, Shimane Y, Usui K, Tokuda M, Tame A, Uematsu K, Maruyama T, Hatada Y

    International journal of systematic and evolutionary microbiology   64 ( Pt 11 )   3709 - 16   2014.11（ ISSN:1466-5026 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1099/ijs.0.067199-0

    PubMed

  • Thalassospira alkalitolerans sp. nov. and Thalassospira mesophila sp. nov., isolated from a decaying bamboo sunken in the marine environment, and emended description of the genus Thalassospira. Reviewed

    Tsubouchi T, Ohta Y, Haga T, Usui K, Shimane Y, Mori K, Tanizaki A, Adachi A, Kobayashi K, Yukawa K, Takagi E, Tame A, Uematsu K, Maruyama T, Hatada Y

    International journal of systematic and evolutionary microbiology   64 ( Pt 1 )   107 - 15   2014.01（ ISSN:1466-5026 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1099/ijs.0.056028-0

    PubMed

  • High frequency of phylogenetically diverse reductive dehalogenase-homologous genes in deep subseafloor sedimentary metagenomes. Reviewed

    Kawai M, Futagami T, Toyoda A, Takaki Y, Nishi S, Hori S, Arai W, Tsubouchi T, Morono Y, Uchiyama I, Ito T, Fujiyama A, Inagaki F, Takami H

    Frontiers in microbiology   5   80   2014

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.3389/fmicb.2014.00080

    PubMed

  • Draft Genome Sequence of Loktanella cinnabarina LL-001T, Isolated from Deep-Sea Floor Sediment. Reviewed

    Nishi S, Tsubouchi T, Takaki Y, Koyanagi R, Satoh N, Maruyama T, Hatada Y

    Genome announcements   1 ( 6 )   2013.11

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1128/genomeA.00927-13

    PubMed

  • Molecular biological and isotopic biogeochemical prognoses of the nitrification-driven dynamic microbial nitrogen cycle in hadopelagic sediments. Reviewed

    Nunoura T, Nishizawa M, Kikuchi T, Tsubouchi T, Hirai M, Koide O, Miyazaki J, Hirayama H, Koba K, Takai K

    Environmental microbiology   15 ( 11 )   3087 - 107   2013.11（ ISSN:1462-2912 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/1462-2920.12152

    PubMed

  • Draft Genome Sequence of the Dimorphic Prosthecate Bacterium Brevundimonas abyssalis TAR-001T. Reviewed

    Tsubouchi T, Nishi S, Usui K, Shimane Y, Takaki Y, Maruyama T, Hatada Y

    Genome announcements   1 ( 5 )   2013.10

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1128/genomeA.00826-13

    PubMed

  • Attachment and detachment of living microorganisms using a potential-controlled electrode. Reviewed

    Koyama S, Konishi MA, Ohta Y, Miwa T, Hatada Y, Toyofuku T, Maruyama T, Nogi Y, Kato C, Tsubouchi T

    Marine biotechnology (New York, N.Y.)   15 ( 4 )   461 - 75   2013.08（ ISSN:1436-2228 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s10126-013-9495-2

    PubMed

  • Polycladomyces abyssicola gen. nov., sp. nov., a thermophilic filamentous bacterium isolated from hemipelagic sediment. Reviewed

    Tsubouchi T, Shimane Y, Mori K, Usui K, Hiraki T, Tame A, Uematsu K, Maruyama T, Hatada Y

    International journal of systematic and evolutionary microbiology   63 ( Pt 6 )   1972 - 81   2013.06（ ISSN:1466-5026 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1099/ijs.0.043596-0

    PubMed

  • Brevundimonas abyssalis sp. nov., a dimorphic prosthecate bacterium isolated from deep-subsea floor sediment. Reviewed

    Tsubouchi T, Shimane Y, Usui K, Shimamura S, Mori K, Hiraki T, Tame A, Uematsu K, Maruyama T, Hatada Y

    International journal of systematic and evolutionary microbiology   63 ( Pt 6 )   1987 - 94   2013.06（ ISSN:1466-5026 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1099/ijs.0.043364-0

    PubMed

  • Loktanella cinnabarina sp. nov., isolated from a deep subseafloor sediment, and emended description of the genus Loktanella. Reviewed

    Tsubouchi T, Shimane Y, Mori K, Miyazaki M, Tame A, Uematsu K, Maruyama T, Hatada Y

    International journal of systematic and evolutionary microbiology   63 ( Pt 4 )   1390 - 5   2013.04（ ISSN:1466-5026 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1099/ijs.0.043174-0

    PubMed

  • The Hadal Amphipod Hirondellea gigas possessing a unique cellulase for digesting wooden debris buried in the deepest seafloor. Reviewed

    Kobayashi H, Hatada Y, Tsubouchi T, Nagahama T, Takami H

    PloS one   7 ( 8 )   e42727   2012

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1371/journal.pone.0042727

    PubMed

  • Involvement of the arginine repressor in lysine biosynthesis of Thermus thermophilus. Reviewed

    Fujiwara K, Tsubouchi T, Kuzuyama T, Nishiyama M

    Microbiology (Reading, England)   152 ( Pt 12 )   3585 - 3594   2006.12（ ISSN:1350-0872 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1099/mic.0.29222-0

    PubMed

  • Leader peptide-mediated transcriptional attenuation of lysine biosynthetic gene cluster in Thermus thermophilus. Reviewed

    Tsubouchi T, Mineki R, Taka H, Kaga N, Murayama K, Nishiyama C, Yamane H, Kuzuyama T, Nishiyama M

    The Journal of biological chemistry   280 ( 18 )   18511 - 6   2005.05（ ISSN:0021-9258 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1074/jbc.M414456200

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MISC

  • 当院で分離された多剤耐性Acinetobacter baumannii株の完全長ゲノム解析から見出した知見

    藤本 凜太郎, 坪内 泰志, 鈴木 仁人, 仁木 誠, 仁木 満美子, 老沼 研一, 山田 康一, 掛屋 弘, 金子 幸弘

    緑膿菌感染症研究会講演記録   55回   21 - 23   2021.09（ ISSN:1340-6477 ）

  • Acinetobacter baumannii OCU_Ac16aが保持するblaTMB-1とblaOXA-58の機能性の検討

    榮山 新, 老沼 研一, 鈴木 仁人, 佐伯 康匠, 坪内 泰志, 仁木 満美子, 山田 康一, 柴山 恵吾, 掛屋 弘, 金子 幸弘

    緑膿菌感染症研究会講演記録   55回   18 - 19   2021.09（ ISSN:1340-6477 ）

  • Klebsiella pneumoniae菌血症の30日後死亡予測因子の解析 遺伝子学的特徴も含めて

    並川 浩己, 仁木 誠, 仁木 満美子, 山田 康一, 中家 清隆, 榮山 新, 老沼 研一, 坪内 泰志, 金子 幸弘, 首藤 太一, 掛屋 弘

    感染症学雑誌   94 ( 3 )   426 - 427   2020.05（ ISSN:0387-5911 ） （ eISSN:1884-569X ）

  • 嫌気性菌研究の最前線 真菌のバイオフィルム形成における嫌気性菌の役割

    仁木 満美子, 山入 和志, 老沼 研一, 坪内 泰志, 掛屋 弘, 金子 幸弘

    日本嫌気性菌感染症学会雑誌   50 ( 1 )   23 - 23   2020.03（ ISSN:2188-7365 ）

  • 【何が同じで、どこが違う?現場向けESBL産生菌・CRE(CPE)対策】Important ESBL産生菌・CRE(CPE)対策のための3大知見 両耐性菌の共通・相違の視点から 定義と分類の最新知見 現場で必須の知識・役立つ知識

    佐伯 康匠, 坪内 泰志, 金子 幸弘

    (株)ヴァンメディカル 感染対策ICTジャーナル   14 ( 3 )   189 - 194   2019.07（ ISSN:1881-4964 ）

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    Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)  

  • hypermucoviscous Klebsiella pneumoniaeによる菌血症の臨床的特徴と予測因子の解析

    並川 浩己, 榮山 新, 井本 和紀, 山入 和志, 柴多 渉, 吉井 直子, 山田 康一, 中家 清隆, 老沼 研一, 仁木 満美子, 金子 幸弘, 首藤 太一, 掛屋 弘, 坪内 泰志

    感染症学雑誌   93 ( 2 )   181 - 182   2019.03（ ISSN:0387-5911 ） （ eISSN:1884-569X ）

  • 電位印加を利用した深海底堆積物由来放線菌の電気回収技術の開発

    小山純弘, 西真郎, 長野由梨子, 多米晃裕, 植松勝之, 秦田勇二, 坪内泰志, 能木裕一

    電気化学会大会講演要旨集(CD-ROM)   84th   2017

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  • ITO電極による深海底堆積物由来ストレプトマイセス属微生物の電気回収

    小山純弘, 西真郎, 長野由梨子, 能木裕一, 秦田勇二, 坪内泰志

    日本生物工学会大会講演要旨集   68th   2016

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  • ITO電極による深海放線菌の電気回収

    小山純弘, 西真郎, 長野由梨子, 能木裕一, 秦田勇二, 坪内泰志

    電気化学会大会講演要旨集(CD-ROM)   83rd   2016

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  • Brevundimonas abyssalisが有する薬剤耐性機構の解析

    坪内泰志, 西真郎, 秦田勇二

    マリンバイオテクノロジー学会大会講演要旨集   18th   2016

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  • Analysis of metabolites from woody biomass extract by a marine Novosphingobium capable of cleaving β-O-4 ether linkages in a lignin model dimer

    Kanako Kurosawa, Taishi Tsubouchi, Yuji Hatada, Hiroshi Nishimura, Takashi Watanabe, Ryoichi Hasegawa, Yukari Ohta

    Proceedings of the 60th Lignin Symposium, Tsukuba, Japan   130 - 131   2015.11

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    Publishing type：Research paper, summary (national, other academic conference)  

  • リグニンモデル2量体中β-O-4結合を開裂する海洋性Novosphingobium属細菌のゲノムおよびトランスクリプトーム解析

    西真郎, 黒澤佳奈子, 小林樹和, 坪内泰志, 秦田勇二, 大田ゆかり

    リグニン討論会講演集   60th   2015

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  • 深海堆積物から単離したBrevundimonas属微生物のゲノム解析

    徳田真紀, 坪内泰志, 西真郎, 小山純弘, 多米晃裕, 植松勝之, 秦田勇二

    ブルーアース要旨集   2015   2015

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  • 透明電極による生きた微生物の付着および剥離の電気制御

    小山純弘, 小西正朗, 大田ゆかり, 三輪哲也, 秦田勇二, 豊福高志, 丸山正, 能木裕一, 加藤千明, 坪内泰志

    マリンバイオテクノロジー学会大会講演要旨集   16th   20   2014.05

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    Publishing type：Research paper, summary (national, other academic conference)  

    J-GLOBAL

  • 透明電極基板を利用した酵母のマイクロパターニング技術の開発

    小山純弘, 坪内泰志, 臼井けい子, 植松勝之, 多米晃裕, 能木裕一, 大田ゆかり, 秦田勇二, 加藤千明, 長濱統彦, 小西正朗, 豊福高志, 三輪哲也, 阿部文快, 長野由梨子, 丸山正

    電気化学会大会講演要旨集   81st   261   2014.03

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    Publishing type：Research paper, summary (national, other academic conference)  

    J-GLOBAL

  • ITO電極を用いた環境微生物の電気回収法

    小山純弘, 小西正朗, 大田ゆかり, 三輪哲也, 秦田勇二, 豊福高志, 丸山正, 能木裕一, 加藤千明, 坪内泰志

    日本化学会講演予稿集   94th ( 3 )   874   2014.03（ ISSN:0285-7626 ）

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    Publishing type：Research paper, summary (national, other academic conference)  

    J-GLOBAL

  • ITO電極基板を用いた効率的な環境微生物の回収法

    小山純弘, 小西正朗, 大田ゆかり, 三輪哲也, 秦田勇二, 豊福高志, 丸山正, 能木裕一, 加藤千明, 坪内泰志

    ブルーアース要旨集   2014   81 - 82   2014

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    Publishing type：Research paper, summary (national, other academic conference)  

    J-GLOBAL

  • 1P-255 Electrical retrieval for yeast S. cerevisiae using an optically transparent electrode.

    KOYAMA Sumihiro, TSUBOUCHI Taishi, NOGI Yuichi, USUI Keiko, UEMATSU Katsuyuki, TAME Akihiro, ITO Takahiro, KOBAYASHI Kenji, OHTA Yukari, KATO Chiaki, NAGAHAMA Takahiko, KONISHI Masaaki, TOYOFUKU Takashi, MIWA Tetsuya, ABE Fumiyoshi, NAGANO Yuriko, HATADA Yuji, MARUYAMA Tadashi

    日本生物工学会, 日本生物工学会大会講演要旨集   66   2014

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    Publishing type：Research paper, summary (national, other academic conference)  

    CiNii Article

  • リグニン代謝能を有すNovosphingobium属細菌のゲノム解析

    西真郎, 大田ゆかり, 飯田加賀美, 小林樹和, 長谷川良一, 黒澤佳奈子, 谷崎明子, 足立明子, 佐藤玲央奈, 芳賀拓真, 坪内泰志, 丸山正, 秦田勇二

    日本ゲノム微生物学会年会要旨集   8th   2014

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  • ITO電極を利用した生きた深海微生物の電気回収

    小山純弘, 小西正朗, 大田ゆかり, 三輪哲也, 秦田勇二, 豊福高志, 丸山正, 能木裕一, 加藤千明, 坪内泰志

    高圧討論会講演要旨集   54th   284   2013.10（ ISSN:0917-6373 ）

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    J-GLOBAL

  • ITO電極による生きた微生物の付着および剥離の電気制御

    小山純弘, 小西正朗, 大田ゆかり, 三輪哲也, 秦田勇二, 豊福高志, 丸山正, 能木裕一, 加藤千明, 坪内泰志

    マリンバイオテクノロジー学会大会講演要旨集   15th   61   2013.06

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    Publishing type：Research paper, summary (national, other academic conference)  

    J-GLOBAL

  • PJ-004 海洋堆積物中に優占するCandidate division JS1は潜在的ホモアセトジェン(ゲノミクス・メタゲノミクス,ポスター発表)

    高見 英人, 豊田 敦, 高木 善弘, 荒井 渉, 谷口 丈晃, 西 真郎, 坪内 泰志, 河合 幹彦, 藤山 秋佐夫, 稲垣 史生

    日本微生物生態学会講演要旨集   ( 29 )   134 - 134   2013

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  • PM-010 ゲノム解析から見出されたBrevundimonas abyssalisの細胞接着メカニズム(界面・バイオフィルム,ポスター発表)

    坪内 泰志, 西 真郎, 臼井 けい子, 丸山 正, 秦田 勇二

    日本微生物生態学会講演要旨集   ( 29 )   150 - 150   2013

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  • 耐冷耐アルカリ性微生物Brevundimonas abyssalisのゲノム解析

    坪内泰志, 西真郎, 臼井けい子, 丸山正, 秦田勇二

    マリンバイオテクノロジー学会大会講演要旨集   15th   2013

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  • 超深海底堆積物における窒素循環

    布浦拓郎, 西澤学, 坪内泰志, 平井美穂, 小出修, 宮崎淳一, 菊池徹, 木庭啓介, 高井研

    ブルーアース要旨集   2012   67   2012

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    Publishing type：Research paper, summary (national, other academic conference)  

    J-GLOBAL

  • 3Ca02 Property of novel cellulase produced by the deepest-sea animal

    Kobayashi Hideki, Hatada Yuji, Tsubouchi Taishi, Takami Hideto

    64   119 - 119   2012

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  • 深海沈木から単離したバクテリアの分類学的位置と芳香族モノマー代謝

    大田ゆかり, 西真郎, 芳賀拓真, 坪内泰志, 丸山正, 長谷川良一, 秦田勇二

    リグニン討論会講演集   57th   2012

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  • P-140 Metabolic potential of deep subsurface biosphere revealed by metagenomic analysis(Poster Session)

    TAKAKI YOSHIHIRO, NISHI SHINRO, TOYODA ATUSHI, ITOH TAKEHIKO, ARAI WATARU, TSUBOUCHI TAISHI, MORONO YUUKI, INAGAKI FUMIO, AOIKE KAN, TAKAI KEN, TAKAMI HIDETO

    日本微生物生態学会, 日本微生物生態学会講演要旨集   ( 26 )   164 - 164   2010

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    Publishing type：Research paper, summary (national, other academic conference)  

    CiNii Article

  • P-1 Microbial communities associated with hadopelagic sediments at the Ogasawara Trench(Poster Session)

    HIRAI MIHO, NUNOURA TAKURO, KIKUCHI TOHRU, TSUBOUCHI TAISHI, KOIDE OSAMU, NISHIZAWA MANABU, MIYAZAKI JUNICHI, TAKAI KEN

    日本微生物生態学会, 日本微生物生態学会講演要旨集   ( 26 )   2010

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    Publishing type：Research paper, summary (national, other academic conference)  

    CiNii Article

  • P-119 Does 365 meters vertical profile of oceanic sediments afford cues to solve the correlation between archaea and their habitat?(Poster Session)

    TSUBOUCHI TAISHI, TOYODA ATSUSHI, ARAI WATARU, TAKAKI YOSHIHIRO, AOIKE KAN, NISHI SHINRO, TAKAMI HIDETO

    ( 26 )   154 - 154   2010

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  • P-139 Microbial ecosystem revealed by metagenomic analysis of the deepest sea mud of the Mariana Trench(Poster Session)

    NISHI SHINRO, TAKAKI YOSHIHIRO, TUBOUTHI TAISHI, ARAI WATARU, OGURA YOSHITOSHI, HAYASHI TETSUYA, TAKAMI HIDETO

    ( 26 )   164 - 164   2010

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  • 2A-2 メタゲノム解析から掘削コアの遺伝子多様性(口頭発表)

    高見 英人, 豊田 敦, 伊藤 武彦, 坪内 泰志, 西 真郎, 高木 善弘, 荒井 渉, 堀 沙耶香, 稲垣 史生, 諸野 祐樹, 青池 寛, 高井 研

    日本微生物生態学会　日本微生物生態学会講演要旨集   ( 25 )   35 - 35   2009

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    Publishing type：Research paper, summary (national, other academic conference)  

    CiNii Article

  • 09-161 Bacterial population based on 16SrRNA genes of deep subsurface core sample collected in an offing of the Simokita Peninsula(Classification/Phylogenetic analysis)

    Nishi Shinro, Toyoda Atsushi, Matsui Satomi, Tsubouchi Taishi, Hori Sayaka, Arai Wataru, Morono Yuki, Inagaki Fumio, Takai Ken, Takami Hideto

    日本微生物生態学会, 日本微生物生態学会講演要旨集   ( 24 )   139 - 139   2008

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    Publishing type：Research paper, summary (national, other academic conference)  

    CiNii Article

  • 02-040 Metagenomic analysis of deep subsurface core samples collected in an offing of the Shimokita Peninsula on the northwest Honshu, Japan(Soil ecosystem)

    Takami Hideto, Toyoda Atsushi, Itoh Tekehiko, Tsubouchi Taishi, Matsui Satomi, Hori Sayaka, Nishi Shinrou, Arai Wataru, Morono Yuki, Inagaki Fumio, Takai Ken

    日本微生物生態学会, 日本微生物生態学会講演要旨集   ( 24 )   78 - 78   2008

    　More details

    Publishing type：Research paper, summary (national, other academic conference)  

    CiNii Article

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Presentations

▼display all

Industrial Property Rights

  • 土壌微生物の調製方法およびその利用

    小山　純弘，坪内　泰志

    　More details

    property_type：Patent 

    Application no：2012-055306 

  • 生きた微生物の固定化方法および調製方法

    小山　純弘，坪内　泰志

    　More details

    property_type：Patent 

    Application no：2011-010693 

Collaborative research (seeds) keywords

  • Deep Sea Buried Bacteria Project

    Category：Life Science 

Outline of collaborative research (seeds)

  • Deep Sea Buried Bacteria Project

    　More details

    Type of research exchange：Joint research

    Application fields / methods etc：drug discovery

    We are efficiently isolating unidentified actinomycete strains from the deep sea environment (sediments and sponges) and searching for substances with antibacterial and anticancer effects.
    To date, multiple antibacterial, antiviral, and anticancer seeds have been obtained.
    We are looking for collaborative research with challenging researchers who are interested in research to discover new physiologically active substances.

Grant-in-Aid for Scientific Research

  • 深海性放線菌が生産する天然物に着目したスキルス胃癌を特異的に排除する抗癌剤の開発

    Grant-in-Aid for Scientific Research(B)  2023.04

  • Characterization of a novel anti-Cryptococcus agent derived from deep-sea Actinomycetes

    Grant-in-Aid for Scientific Research(C)  2026

  • Characterization of a novel anti-Cryptococcus agent derived from deep-sea Actinomycetes

    Grant-in-Aid for Scientific Research(C)  2025

  • Characterization of a novel anti-Cryptococcus agent derived from deep-sea Actinomycetes

    Grant-in-Aid for Scientific Research(C)  2024

  • Creation of Active Substances from Deep-sea Actinomycetes to Disrupt Drug-Resistant Acinetobacter spp.

    Grant-in-Aid for Challenging Research (Exploratory)  2024

  • Development of anti-scirrhous stomach cancer produced by the deep-sea actinomyces

    Grant-in-Aid for Scientific Research(B)  2024

  • 深海放線菌が産生する新規の胃癌抑制物質の同定

    Grant-in-Aid for Scientific Research(C)  2024

  • 深海放線菌が産生する新規の胃癌抑制物質の同定

    Grant-in-Aid for Scientific Research(C)  2023.04

  • 薬剤耐性アシネトバクター属細菌を打ち砕く深海性放線菌由来活性物質の創製

    Grant-in-Aid for Challenging Research (Pioneering)/(Exploratory)  2023.04

  • 偏性嫌気性菌-真菌共存下における病原因子発現調節の解析

    Grant-in-Aid for Scientific Research(C)  2023.04

  • 深海放線菌由来の新規抗Candida auris物質の特性解析

    Grant-in-Aid for Scientific Research(C)  2023.04

  • 薬剤耐性アシネトバクター属細菌を打ち砕く深海性放線菌由来活性物質の創製

    Grant-in-Aid for Challenging Research (Pioneering)/(Exploratory)  2022.07

  • 深海放線菌が産生する新規の胃癌抑制物質の同定

    Grant-in-Aid for Scientific Research(C)  2022.04

  • 偏性嫌気性菌-真菌共存下における病原因子発現調節の解析

    Grant-in-Aid for Scientific Research(C)  2022.04

  • 深海放線菌由来の新規抗Candida auris物質の特性解析

    Grant-in-Aid for Scientific Research(C)  2022.04

  • 深海性放線菌が生産する天然物に着目したスキルス胃癌を特異的に排除する抗癌剤の開発

    Grant-in-Aid for Scientific Research(B)  2022.04

  • 深海性放線菌が生産する天然物に着目したスキルス胃癌を特異的に排除する抗癌剤の開発

    Grant-in-Aid for Scientific Research(B)  2021.04

  • Hunting of antibacterial agent, 2nd. Development of novel antibacterial agent which drives multiple-drug-resistant bacteria away.

    Grant-in-Aid for Scientific Research(C)  2017.04

  • Hunting of antibacterial agent, acquirement of novel antibacterial agent which drives multiple-drug-resistant bacteria away.

    Grant-in-Aid for Scientific Research(C)  2014.04

  • Functional analysis and application of Lignin beta-O-4 bond cleavage enzymes

    Grant-in-Aid for Scientific Research(C)  2013.04

  • Methane synthesis using the budding yeast expression system

    Grant-in-Aid for Challenging Research (Pioneering)/(Exploratory)  2012.04

  • Preparation method of environmental DNA sample for conservation of the genetic resource in the deep subsurface biosphere and analysis of biodiviersity harbored in the DNA

    Grant-in-Aid for Scientific Research(B)  2008

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Contract research

  • 薬剤耐性菌対策に資する診断法・治療法等の開発研究

    国立研究開発法人日本医療研究開発機構  2020.04

  • Development of the novel antibacterial agent for multidrug resistant bacteria, derived from deepsea actinomycetales

    Japan Agency for Medical Research and Development/Kyoto University Hospital  2018.04

  • 薬剤耐性菌対策に資する診断法・治療法等の開発研究

    国立研究開発法人日本医療研究開発機構  2017.04

  • 海洋微生物酵素群によるリグニン分解高度化と人工漆材料への展開

    日本科学技術振興機構  2015

Incentive donations / subsidies

  • 薬剤耐性菌対策に資する診断法・治療法等の開発研究

    国立研究開発法人　日本医療研究開発機構  新興・再興感染症に対する革新的医薬品等開発推進研究事業　  2021.04

  • 薬剤耐性菌対策に資する診断法・治療法等の開発研究

    国立研究開発法人　日本医療研究開発機構  新興・再興感染症に対する革新的医薬品等開発推進研究事業  2017.04

Outline of education staff

  • 細菌学セミナー
    細菌学セミナーでは、医学に関連した細菌学的知識および細菌学研究で用いる手法について修得する。また、学生の演習として、論文等の情報の調査と発表を実践し、論文作成までの手順を学ぶ。
    （オムニバス形式／講義・演習　全45回）（単位認定者：金子幸弘）

    細菌学研究演習
    細菌学研究演習では、各担当者が支援しながら、研究の目的、研究デザイン、研究方法、研究実施手順、結果の解釈、研究倫理について、学生自らに考えさせる。研究の意義や限界について、それぞれの視点から考えられる能力を修得する。
    （オムニバス形式／全15回）（単位認定者：金子幸弘）

    感染症基礎教育
    研究を進める上で基盤となる感染症一般について、感染制御、病態理解、抗菌薬治療、小児領域感染症など異なるアプローチの知識を習得する。
    （オムニバス形式・共同／講義・演習全30回）（単位認定者：掛屋弘）

    細菌学実習
    微生物を取扱う際に必要な知識を修得し、実践する。
    （金子幸弘／3回）
    バイオセーフティ講習を行い、安全な微生物の取扱を実践できるようにする。

    研究指導
    研究指導では、各自の研究テーマに従って立案、遂行、得られた結果の解析、考察をおこない、論文として完成させ、投稿、受理されるまでのプロセスを一貫して教授する。細菌学研究の医学における意義について理解し、応用できる能力を修得できるように指導する。

Charge of on-campus class subject

  • 病理病態学概論

    2024   Weekly class   Graduate school

  • 病理病態学概論

    2023   Weekly class   Graduate school

  • 人体を考える

    2023   Weekly class   Undergraduate

  • 医学研究推進コース3

    2023   Practical Training   Undergraduate

  • 人体を考える

    2022   Weekly class   Undergraduate

  • 病理病態学概論

    2022   Weekly class   Graduate school

  • 医学研究推進コース3

    2022   Practical Training   Undergraduate

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Charge of off-campus class subject

  • Environment and Health

    2020.04
    -
    Now
    Institution：Osaka City University

    　More details

    Level：Undergraduate (liberal arts) 

  • Introduction to biology (M)

    2019.01
    -
    Now
    Institution：Osaka City University

    　More details

    Level：Undergraduate (liberal arts) 

  • Introduction to clinical state and pathology

    2018.06
    -
    Now
    Institution：Osaka City University / Graduate School of Medicine

    　More details

    Level：Postgraduate 

  • Biological control

    2018.01
    -
    Now
    Institution：Tokyo University of Agriculture

    　More details

    Level：Undergraduate (specialized) 

Faculty development activities

  • FD活動への貢献  2023

    　More details

    医学部で実施されているFD講習会に参加した。

  • FD活動への貢献  2022

    　More details

    医学部で実施されているFD講習会に参加

  • FD活動への貢献  2021

    　More details

    医学部で実施されているFD講習会に参加

Number of instructed thesis, researches

  • 2023

    [Number of instructed the Master's Program] (letter term)：2

Original item・Special report (Education Activity)

  • 2023

    　 More details

    Original item：2023年度バイオインフォマティクス・オミックス勉強会

    Special report：【Mac terminal / R(Rstudio)を⽤いたバイオインフォマティクス解析】
    本講座はCUIであるMac terminal / R(RStudio)を⽤いてバイオインフォマティクス解析・オミックス解析を⾏います。Macに実装されているterminalで情報解析を⾏うため、各⾃、事前にR(Rstudio)をインストールしたMac PCを御⽤意ください。
    本年度はhands-on形式ではありませんが、講義はゆっくり進めるのでterminalやRstudioの操作に慣れている⽅は同時に進めてください。

  • 2022

    　 More details

    Original item：2022年度バイオインフォマティクス・オミックス勉強会

    Special report：【Mac terminal / R(Rstudio)を⽤いたバイオインフォマティクス解析】
    本講座はCUIであるMac terminal / R(RStudio)を⽤いてバイオインフォマティクス解析・オミックス解析を⾏います。Macに実装されているterminalで情報解析を⾏うため、各⾃、事前にR(Rstudio)をインストールしたMac PCを御⽤意ください。
    hands-on形式を採用するので、terminalやRstudioの操作に慣れている⽅は積極的に進めてください。

Media Coverage

  • Electrical retrieval of living microorganisms Newspaper, magazine

    The Nikkan Kogyo Shimbun  2014.04

Academic Activities

  • 論文査読（Archives of Microbiology）

    Role(s)： Peer review

    2024.01 - Now

    　More details

    Type：Peer review 

  • 論文査読（JIC）

    Role(s)： Peer review

    2023.09 - Now

    　More details

    Type：Peer review 

Other

  • Job Career

    2020.04 - Now

    　 More details

    Osaka City University Graduate School of Medicine Basic Medicine Course Associate professor

  • Job Career

    2017.07 - 2020.03

    　 More details

    Osaka City University Graduate School of Medicine Basic Medicine Course Specially-appointed Lecturer