Updated on 2024/04/08

写真a

 
MASUI Ryoji
 
Organization
Graduate School of Science Department of Biology Professor
School of Science Department of Biology
Title
Professor
Affiliation
Institute of Science
Affiliation campus
Sugimoto Campus

Position

  • Graduate School of Science Department of Biology 

    Professor  2022.04 - Now

  • School of Science Department of Biology 

    Professor  2022.04 - Now

Degree

  • Ph.D ( Osaka University )

Research Areas

  • Life Science / Structural biochemistry

Research Career

  • DNA 修復システム,ヌクレオチド代謝システム,翻訳後修飾

    翻訳後修飾、DNA修復、ヌクレオチド代謝  Individual

    1993.04 - Now 

Professional Memberships

  • 日本生化学会

      Domestic

  • 日本分子生物学会

      Domestic

  • 日本蛋白質科学会

      Domestic

  • 極限環境生物学会

      Domestic

Committee Memberships (off-campus)

  • 評議員   日本生化学会  

    2017.04 - Now 

Awards

  • 第9回OCARINA国際シンポジウムポスター賞

    2018.03  

  • 第39回日本分子生物学会年会優秀ポスター賞

    2016.11  

  • 第17回極限環境生物学会ポスター賞

    2016.11  

  • 第85回日本生化学会大会 鈴木紘一メモリアル賞

    2012  

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    Country:Japan

  • 第84回日本生化学会大会 鈴木紘一メモリアル賞

    2011  

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    Country:Japan

  • 第10回日本蛋白質科学会年会 ポスター賞

    2010  

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    Country:Japan

  • 第10回日本蛋白質科学会年会 ポスター賞

    2010  

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    Country:Japan

  • 第10回日本蛋白質科学会年会 ポスター賞受

    2010  

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    Country:Japan

  • 日本生化学会 J. Biochem.論文賞

    2009  

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    Country:Japan

  • 第9回日本蛋白質科学会年会 ポスター賞

    2009  

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    Country:Japan

  • 第56回日本生化学会近畿支部例会 優秀発表賞

    2009  

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    Country:Japan

  • 日本生化学会 J. Biochem. 論文賞

    2000  

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    Country:Japan

  • The 11th Symposium of Protein Society (Boston, U.S.A.) Award for Best Posters

    1997  

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Job Career (off-campus)

  • 大阪大学   大学院理学研究科   准教授

    2007.12 - 2014.09

  • 大阪大学   大学院理学研究科   講師

    2002.04 - 2007.11

  • 理化学研究所   播磨研究所   連携研究員

    1999.10 - 2013.03

  • 大阪大学   大学院理学研究科   助手

    1996.04 - 2002.03

  • 大阪大学   理学部   教務員

    1993.04 - 1996.03

Education

  • Osaka University     Graduated/Completed

    1983.04 - 1988.03

Papers

  • Structural and biochemical characterizations of Thermus thermophilus HB8 transketolase producing a heptulose Reviewed

    Mochizuki, A., Yoshida, H., Masui, R., Izumori, K., Kamitori, S.

    Applied Microbiology and Biotechnology   107 ( 1 )   233 - 245   2023.01

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work   International / domestic magazine:International journal  

    DOI: 10.1007/s00253-022-12297-z

    Other URL: https://link.springer.com/article/10.1007/s00253-022-12297-z

  • Crystal structure of a nucleotide-binding domain of fatty acid kinase FakA from Thermus thermophilus HB8 Reviewed

    Nakatani, M., Nakahara, Shun-Ya, Fukui, K., Urano, M., Fujii, Y., Murakawa, T., Baba, S., Kumasaka, T., Okanishi, H., Kanai, Y., Yano, T., Masui. R.

    Journal of Structural Biology   214 ( 4 )   107904 - 107904   2022.12

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    DOI: 10.1016/j.jsb.2022.107904

    Other URL: https://www.sciencedirect.com/science/article/pii/S1047847722000740?via%3Dihub

  • Structural changes induced by ligand-bidning drastically increase the thermostability of the Ser/Thr protein kinase TpkD from Thermus thermophilus HB8 Reviewed

    Fujino, Y., Miyagawa, T., Torii, M., Inoue, M., Fujii, Y., Okanishi, H., Kanai, Y., Masui, R.

    FEBS Letters   595 ( 2 )   264 - 274   2021.01

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • The crystal structures of Thermus thermophilus CMP kinase complexed with a phosphoryl group acceptor and donor Reviewed

    Ryosuke Mega, Noriko Nakagawa, Seiki Kuramitsu, Ryoji Masui

    PLoS ONE   15 ( 3 )   e0233689   2020.05

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

  • Resistance to UV irradiation caused by inactivation of nurA and herA genes in Thermus thermophilus Reviewed

    Fujii, Y., Inoue, M., Fukui, K., Kuramitsu, S., Masui, R.

    Journal of Bacteriology   200 ( 16 )   e00201-18   2018.07( ISSN:0021-9193

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    NurA and HerA are thought to be essential proteins for DNA end resection in archaeal homologous recombination systems. Thermus thermophilus, an extremely thermophilic eubacterium, has proteins that exhibit significant sequence similarity to archaeal NurA and HerA. To unveil the cellular function of NurA and HerA in T. thermophilus, we performed phenotypic analysis of disruptant mutants of nurA and herA with or without DNA-damaging agents. The nurA and herA genes were not essential for survival, and their deletion had no effect on cell growth and genome integrity. Unexpectedly, these disruptants of T. thermophilus showed increased resistance to UV irradiation and mitomycin C treatment. Further, these disruptants and the wild type displayed no difference in sensitivity to oxidative stress and a DNA replication inhibitor. T. thermophilus NurA had nuclease activity, and HerA had ATPase. The overexpression of loss-of-function mutants of nurA and herA in the respective disruptants showed no complementation, suggesting their enzymatic activities were involved in the UV sensitivity. In addition, T. thermophilus NurA and HerA interacted with each other in vitro and in vivo, forming a complex with 2:6 stoichiometry. These results suggest that the NurA-HerA complex has an architecture similar to that of archaeal counterparts but that it impairs, rather than promotes, the repair of photoproducts and DNA cross-links in T. thermophilus cells. This cellular function is distinctly different from that of archaeal NurA and HerA.

    DOI: 10.1128/JB.00201-18

    PubMed

  • Indispensable residue for uridine binding in the uridine-cytidine kinase family Reviewed

    Tomoike, F., Nakagawa, N., Fukui, K., Yano, T., Kuramitsu, S., Masui, R.

    Biochemistry and Biophysics Reports1   11   93 - 98   2017.07

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  • The Lon protease-like domain in the bacterial RecA paralog RadA is required for DNA binding and repair Reviewed

    Inoue Masao, Fukui Kenji, Fujii Yuki, Nakagawa Noriko, Yano Takato, Kuramitsu Seiki, Masui Ryoji

    Journal of Biological Chemistry   292 ( 23 )   9801 - 9814   2017.06( ISSN:0021-9258

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    Homologous recombination (HR) plays an essential role in the maintenance of genome integrity. RecA/Rad51 paralogs have been recognized as an important factor of HR. Among them, only one bacterial RecA/Rad51 paralog, RadA, is involved in HR as an accessory factor of RecA recombinase. RadA has a unique Lon protease-like domain (LonC) at its C terminus, in addition to a RecA-like ATPase domain. Unlike Lon protease, RadA's LonC domain does not show protease activity but is still essential for RadA-mediated DNA repair. Reconciling these two facts has been difficult because RadA's tertiary structure and molecular function are unknown. Here, we describe the hexameric ring structure of RadA's LonC domain, as determined by X-ray crystallography. The structure revealed the two positively charged regions unique to the LonC domain of RadA are located at the intersubunit cleft and the central hole of a hexameric ring. Surprisingly, a functional domain analysis demonstrated the LonC domain of RadA binds DNA, with site-directed mutagenesis showing that the two positively charged regions are critical for this DNA-binding activity. Interestingly, only the intersubunit cleft was required for the DNA-dependent stimulation of ATPase activity of RadA, and at least the central hole was essential for DNA repair function. Our data provide the structural and functional features of the LonC domain and their function in RadA-mediated DNA repair.

    DOI: 10.1074/jbc.M116.770180

    PubMed

  • Proteome-wide identification of lysine propionylation in thermophilic and mesophilic bacteria: Geobacillus kaustophilus, Thermus thermophilus, Escherichia coli, Bacillus subtilis, and Rhodothermus marinus Reviewed

    Okanishi Hiroki, Kim Kwang, Masui Ryoji, Kuramitsu Seiki

    Extremophiles   21 ( 2 )   283 - 296   2017.03( ISSN:1431-0651

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work   International / domestic magazine:International journal  

    DOI: 10.1007/s00792-016-0901-3

  • Proteome-wide identification of lysine succinylation in thermophilic and mesophilic bacteria Reviewed

    Okanishi Hiroki, Kim Kwang, Fukui Kenji, Yano Takato, Kuramitsu Seiki, Masui Ryoji

    Biochimica et Biophysica Acta   1865 ( 2 )   232 - 242   2017.02( ISSN:1570-9639

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    DOI: 10.1016/j.bbapap.2016.11.009

  • A specific single-stranded DNA induces a distinct conformational change in the nucleoid-associated protein HU Reviewed

    Nishida, Y., Ikeya, T., Mikawa, T., Inoue, J., Ito, Y., Shintani, Y., Masui, R., Kuramitsu, S., Takashima, S.

    Biochem. Biophys, Rep.   8   318 - 324   2016.10

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  • A putative adenosine kinase family protein possesses adenosine diphosphatase activity Reviewed

    Tomoike Fumiaki, Tsunetou Akiko, Kim Kwang, Nakagawa Noriko, Kuramitsu Seiki, Masui Ryoji

    Bioscience, Biotechnology, and Biochemistry   80 ( 11 )   2138 - 2143   2016.08( ISSN:0916-8451

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    DOI: 10.1080/09168451.2016.1214532

  • Crystal structures of a subunit of the formyl­glycinamide ribonucleotide amidotransferase, PurS, from Thermus thermophilus, Sulfolobus tokodaii and Methanocaldococcus jannaschii Reviewed

    Watanabe, Y., Yanai, H., Kanagawa, M., Suzuki, S., Tamura, S., Okada, K., Baba, S., Kumasaka, T., Agari, Y., Chen, L., Fu, Z.-Q., Chrzas, J., Wang, B.-C., Nakagawa, N., Ebihara, A., Masui, R., Kuramitsu, S., Yokoyama, S., Sampei, G., Kawai, G.

    Acta Crystallographica Section F Structural Biology Communications   72 ( 8 )   627 - 635   2016.08

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work   International / domestic magazine:International journal  

  • Molecular mechanisms of substrate specificities of uridine-cytidine kinase Reviewed

    Tanaka Wataru, Masui Ryoji, Kuramitsu Seiki, Shigeta Yasuteru, Shoji Mitsuo, Tomoike Fumiaki, Ujiie Yuzuru, Hanaoka Kyohei, Harada Ryuhei, Kayanuma Megumi, Kamiya Katsumasa, Ishida Toyokazu

    Biophysics and Physicobiology   13 ( 0 )   77 - 84   2016.06

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    A uridine-cytidine kinase (UCK) catalyzes the phosphorylation of uridine (Urd) and cytidine (Cyd) and plays a significant role in the pyrimidine-nucleotide salvage pathway. Unlike ordinary ones, UCK from <i>Thermus thermophilus</i> HB8 (ttCK) loses catalytic activity on Urd due to lack of a substrate binding ability and possesses an unusual amino acid, i.e. tyrosine 93 (Tyr93) at the binding site, whereas histidine (His) is located in the other UCKs. Mutagenesis experiments revealed that a replacement of Tyr93 by His or glutamine (Gln) recovered catalytic activity on Urd. However, the detailed molecular mechanism of the substrate specificity has remained unclear. In the present study, we performed molecular dynamics simulations on the wild-type ttCK, two mutant ttCKs, and a human UCK bound to Cyd and three protonation forms of Urd to elucidate their substrate specificity. We found three residues, Tyr88, Tyr/His/Gln93 and Arg152 in ttCKs, are important for recognizing the substrates. Arg152 contributes to induce a closed form of the binding site to retain the substrate, and the N3 atom of Urd needed to be deprotonated. Although Tyr88 tightly bound Cyd, it did not sufficiently bind Urd because of lack of the hydrogen bonding. His/Gln93 complemented the interaction of Tyr88 and raised the affinity of ttCK to Urd. The crucial distinction between Tyr and His or Gln was a role in the hydrogen-bonding network. Therefore, the ability to form both hydrogen-bonding donor and accepter is required to bind both Urd and Cyd.

    DOI: 10.2142/biophysico.13.0_77

    CiNii Article

  • Structural and biochemical studies on the reaction mechanism of uridine-cytidine kinase Reviewed

    Tomoike Fumiaki, Nakagawa Noriko, Kuramitsu Seiki, Masui Ryoji

    Protein Journal   34 ( 6 )   411 - 420   2015.12( ISSN:1572-3887

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    DOI: 10.1007/s10930-015-9636-8

  • Roles of Mn-catalase and a possible heme peroxidase homologue in protection from oxidative stress in Thermus thermophilus Reviewed

    Extremophiles   19 ( 4 )   775 - 785   2015.07

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  • The role of ribonucleases in regulating global mRNA levels in the model organism Thermus thermophilus HB8 Reviewed

    BMC Genomics   15   386   2014.09

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  • Structural insights of post-translational modification sites in the proteome of Thermus thermophilus Reviewed

    15 ( 3 )   137 - 151   2014.09

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  • Lysine propionylation is a prevalent post-translational modification in Thermus thermophilus Reviewed

    13 ( 9 )   2382 - 2398   2014.09

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  • Acetylome with structural mapping reveals the significance of lysine acetylation in Thermus thermophilus Reviewed

    1 ( 9 )   3952 - 3968   2013.09

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  • Crystal structure of the ligand-binding form of nanoRNase from Bacteroides fragilis, a member of the DHH/DHHA1 phosphoesterase family of proteins Reviewed International coauthorship

    587 ( 16 )   2669 - 2674   2013.08

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  • MutS stimulates the endonuclease activity of MutL in an ATP-hydrolysis-dependent manner Reviewed

    280 ( 14 )   3467 - 3479   2013.07

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  • Unique substrate specificity of purine nucleoside phosphorylases from Thermus thermophilus Reviewed

    Extremophiles   17 ( 3 )   505 - 514   2013.05

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  • Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus Reviewed

    9 ( 4 )   277 - 283   2013.04

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  • Alkyltransferase-like protein (Atl1) distinguishes alkylated guanines for DNA repair using cation-pi interactions Reviewed International coauthorship

    109 ( 46 )   18755 - 18760   2012.11

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  • The role of the PHP domain associated with DNA polymerase X from Thermus thermophilus HB8 in base excision repair Reviewed

    DNA Repair   11 ( 11 )   906 - 914   2012.11

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  • Whole-cell proteome reference maps of an extreme thermophile, Thermus thermophilus HB8 Reviewed

    Proteomics   12 ( 19-20 )   3063 - 3068   2012.10

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  • Close proximity of phosphorylation sites to ligand in the phosphoproteome of the extreme thermophile Thermus thermophilus HB8 Reviewed

    Proteomics   12 ( 9 )   1414 - 1430   2012.05

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  • A non-cold-inducible cold shock protein homolog mainly contributes to translational control under optimal growth conditions Reviewed

    279 ( 6 )   1014 - 1029   2012.03

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  • The structural basis of the kinetic mechanism of a gap-filling X-family DNA polymerase that binds Mg2+-dNTP before binding to DNA Reviewed

    417 ( 3 )   179 - 196   2012.03

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  • In vivo, in vitro, and X-ray crystallographic analyses suggest the involvement of an uncharacterized TIM barrel protein in protection against oxidative stress Reviewed

    286 ( 48 )   41636 - 41646   2011.12

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  • Commensal symbiotic interrelationship for the growth of Symbiobacterium toebii with its partner bacterium, Geobacillus toebii Reviewed International coauthorship

    BMC Research Notes   4   437   2011.10

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  • Characterization of C- and N-terminal domains of Aquifex aeolicus MutL endonuclease: N-terminal domain stimulates the endonuclease activity of C-terminal domain in a zinc-dependent manner Reviewed

    31 ( 5 )   309 - 322   2011.10

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  • An alkyltransferase-like protein from Thermus thermophilus HB8 affects the regulation of gene expression in alkylation response Reviewed

    J. Biochem.   150 ( 3 )   327 - 339   2011.09

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  • A single amino acid limits the substrate specificity of Thermus thermophilus uridine-cytidine kinase to cytidine Reviewed

    Biochemistry   50 ( 21 )   4597 - 4607   2011.05

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  • Inactivation of DNA repair genes mutS and mutL or the anti-recombination gene mutS2 leads to activation of vitamin B1 biosynthesis genes in Thermus thermophilus HB8 Reviewed

    PLoS One   6 ( 4 )   e19053   2011.04

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  • An additional C-terminal loop in endonuclease IV, an apurinic/apyrimidinic endonuclease, controls binding affinity to DNA Reviewed

    67 ( 3 )   149 - 155   2011.03

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  • Role of alkyltransferase-like (ATL) protein in repair of methylated DNA lesions in Thermus thermophilus Reviewed

    Mutagenesis   26 ( 2 )   303 - 308   2011.03

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  • Role of RecJ-like exonuclease with 5'-3' exonuclease in oligo(deoxy)nucleotide degradation Reviewed

    286 ( 4 )   2807 - 2816   2011.01

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  • A novel single-stranded DNA-specific 3'-5' exonuclease, Thermus thermophilus exonuclease I, is involved in several DNA repair pathways Reviewed

    38 ( 17 )   5692 - 5705   2010.09

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  • Very rapid induction of a cold shock protein by temperature downshift in Thermus thermophilus Reviewed

    399 ( 3 )   336 - 340   2010.08

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  • Crystal structure of an archaeal cleavage and polyadenylation specificity factor subunit from Pyrococcus horikoshii Reviewed

    Proteins   78 ( 10 )   2395 - 2398   2010.08

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  • The first crystal structure of an archaeal metallo-β-lactamase superfamily protein; ST1585 from Sulfolobus tokodaii Reviewed

    Proteins   78 ( 10 )   2399 - 2402   2010.08

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  • Structure of ST0929, a putative glycosyl transferase from Sulfolobus tokodaii Reviewed

    66 ( 4 )   397 - 400   2010.04

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  • Structure of RecJ exonuclease defines its specificity for single-stranded DNA Reviewed

    285 ( 13 )   9762 - 9769   2010.03

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  • Crystal structure of the conserved hypothetical protein TTHA1606 from Thermus thermophilus HB8 Reviewed

    76 ( 1 )   244 - 248   2009.07

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  • Degradation of ppGpp by nudix pyrophosphatase modulates the transition of growth phase in the bacterium Thermus thermophilus Reviewed

    284 ( 23 )   15549 - 15556   2009.06

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  • Two dNTP triphosphohydrolases from Pseudomonas aeruginosa possess diverse substrate specificities Reviewed

    276 ( 12 )   3211 - 3221   2009.06

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  • Characterization of DNA polymerase X from Thermus thermophilus HB8 reveals the POLXc and PHP domains are both required for 3'-5' exonuclease activity Reviewed

    37 ( 6 )   2037 - 2052   2009.04

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  • Crystal structure of MutS2 endonuclease domain and the mechanism of homologous recombination supression Reviewed

    283 ( 48 )   33417 - 33427   2008.11

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  • Crystal structure of a putative DNA methylase TTHA0409 from Thermus thermophilus HB8 Reviewed

    Proteins   73 ( 1 )   259 - 264   2008.10

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  • Bound nucleotide controls the endonuclease activity of mismatch repair enzyme MutL Reviewed

    283 ( 18 )   12136 - 12145   2008.05

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  • Isolation of uncultivable anaerobic thermophiles of the family Clostridiaceae requiring growth-supporting factors Reviewed International coauthorship

    18 ( 4 )   611 - 615   2008.04

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  • Characterization of growth-supporting factors produced by Geobacillus toebii for the commensal thermophile Symbiobacterium toebii. Reviewed International coauthorship

    18 ( 3 )   490 - 496   2008.03

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  • Insights into different dependence of dNTP triphosphohydrolase on metal ion species from intracellular ion concentrations in Thermus thermophilus Reviewed

    Extremophiles   12 ( 2 )   217 - 223   2008.03

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  • An O6-methylguanine-DNA methyltransferase-like protein from Thermus thermophilus interacts with a nucleotide excision repair protein Reviewed

    J. Biochem.   144 ( 2 )   267 - 277   2008.02

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  • Structural basis for different substrate specificities of two ADP-ribose pyrophosphatases from Thermus thermophilus HB8 Reviewed

    190 ( 3 )   1108 - 1117   2008.02

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  • Crystal structure of family 5 uracil-DNA glycosylase bound to DNA Reviewed

    373 ( 4 )   839 - 850   2007.11

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  • Analysis of a nuclease activity of catalytic domain of Thermus thermophilus MutS2 by high-accuracy mass spectrometry Reviewed

    35 ( 15 )   e100   2007.08

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  • Simultaneous detection of N-terminal fragment ions in a protein mixture using a ruthenium(II) complex Reviewed

    21 ( 16 )   2647 - 2653   2007.07

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work   International / domestic magazine:International journal  

  • High sequence-coverage detection of proteolytic peptides using a bis(terpyridine)ruthenium(II) complex Reviewed

    Analyst   132 ( 4 )   358 - 364   2007.04

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  • Structure of archaeal glyoxylate reductase from Pyrococcus horikoshii OT3 complexed with nicotinamide adenine dinucleotide phosphate Reviewed

    63 ( 3 )   357 - 365   2007.03

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  • Rapid SNP diagnostics using asymmetric isothermal amplification and a new mismatch-suppression technology Reviewed

    4 ( 3 )   257 - 262   2007.03

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  • Structure of dNTP-inducible dNTP triphosphohydrolase: insight into broad specificity for dNTPs and triphosphohydrolase-type hydrolysis Reviewed

    63 ( 2 )   230 - 239   2007.02

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  • Nuclease activity of the MutS homologue MutS2 from Thermus thermophilus is confined to the Smr domain Reviewed

    35 ( 3 )   850 - 856   2007.01

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  • Crystal structure of TTHA0252 from Thermus thermophilus HB8, a RNA degradation protein of the metallo-beta-lactamase superfamily Reviewed

    140 ( 4 )   535 - 542   2006.10

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  • Crystal structure of hypothetical protein TTHB192 from Thermus thermophilus HB8 reveals a new protein family with an RNA recognition motif-like domain Reviewed

    15 ( 6 )   1494 - 1499   2006.06

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  • Improved heterologous gene expression in Escherichia coli by optimization of the AT-content of codons immediately downstream of the initiation codon Reviewed

    120 ( 4 )   341 - 346   2005.12

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  • Distinction of Leu and Ile Using a Ruthenium(II) complex by MALDI-LIFT-TOF/TOF-MS analysis Reviewed

    77 ( 20 )   6618 - 6624   2005.10

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  • Functional reconstitution of a crenarchaeal splicing endonuclease in vitro Reviewed

    334 ( 4 )   1254 - 1259   2005.09

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  • Novel reaction mechanism of GTP cyclohydrolase I. High-resolution X-ray crystallography of Thermus thermophilus HB8 enzyme complexed with a transition state analogue, the 8-oxoguanine derivative Reviewed

    138 ( 3 )   263 - 275   2005.09

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  • Molecular mechanism of the Thermus thermophilus ADP-ribose pyrophosphatase from mutational and kinetic studies Reviewed

    Biochemistry   44 ( 26 )   9320 - 9329   2005.07

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  • Inference of S-system models of genetic networks using a cooperative coevolutionary algorithm Reviewed

    Bioinformatics   21 ( 7 )   1154 - 1163   2005.04

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  • Structure of P-protein of the glycine cleavage system: implications for nonketotic hyperglycinemia Reviewed

    24 ( 8 )   1523 - 1536   2005.04

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  • Structure-based functional identification of a novel heme-binding protein from Thermus thermophilus HB8 Reviewed

    6 ( 1 )   21 - 32   2005.04

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  • Inhibition of Thermus thermophilus HB8 thioredoxin activity by platinum(ii) Reviewed

    6   1023 - 1026   2005.03

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  • Multidrug transporter MexB of Pseudomonas aeruginosa: overexpression, purification, and initial structural characterization Reviewed

    40 ( 1 )   91 - 100   2005.03

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  • A novel metal-activated L-serine O-acetyltransferase from Thermus thermophilus HB8 Reviewed

    136 ( 5 )   629 - 634   2004.11

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  • Rapid and sensitive amino-acid sequencing of cloning Thermusthermophilus HB8 ferredoxin by proteomics Reviewed

    3 ( 5 )   983 - 987   2004.10

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  • Biochemical characterization of TT1383 from Thermus thermophilus identifies a novel dNTP triphosphohydrolase activity stimulated by dATP and dTTP Reviewed

    136 ( 2 )   221 - 231   2004.08

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  • Structural insights into the Thermus thermophilus ADP-ribose pyrophosphatase mechanism via crystal structures with the bound substrate and metal. Reviewed

    279 ( 35 )   37163 - 37174   2004.08

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  • Crystal structure of the native chaperonin complex from Thermus thermophilus revealed unexpected asymmetry at the cis-cavity Reviewed

    Structure   12 ( 8 )   1471 - 1480   2004.08

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  • The Nudix hydrolase Ndx1 from Thermus thermophilus HB8 is a diadenosine hexaphosphate hydrolase with a novel activity Reviewed

    279 ( 21 )   21732 - 21739   2004.05

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  • Ligand-induced conformational changes and a reaction intermediate in branched-chain 2-oxo acid dehydrogenase (E1) from Thermus thermophilus HB8, as revealed by X-ray crystallography Reviewed

    337 ( 4 )   1011 - 1033   2004.04

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  • Thermus thermophilus MutS2, a MutS paralogue, possesses an endonuclease activity effected by MutL Reviewed

    135 ( 3 )   375 - 384   2004.03

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  • Trigger factor from Thermus thermophilus is a Zn2+-dependent chaperone Reviewed

    279 ( 8 )   6380 - 6384   2004.02

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  • Crystal structure of a family 4 uracil-DNA glycosylase from Thermus thermophilus HB8 Reviewed

    333 ( 3 )   515 - 526   2003.10

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  • Overproduction, crystallization and preliminary diffraction data of ADP-ribose pyrophosphatase from Thermus thermophilus HB8 Reviewed

    59 ( 10 )   1840 - 1842   2003.10

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  • Crystallization and preliminary X-ray diffraction studies of nucleoside diphosphate kinase from Thermus thermophilus HB8 Reviewed

    59 ( 10 )   1843 - 1845   2003.10

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  • Coexpression, purification, crystallization and preliminary X-ray characterization of glycine decarboxylase (P-protein) of the glycine-cleavage system from Thermus thermophilus HB8 Reviewed

    59 ( 9 )   1610 - 1618   2003.09

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  • Structure of Thermus thermophilus HB8 H-protein of the glycine-cleavage system, resolved by a six-dimensional molecular-replacement method Reviewed

    Nakai, T., Ishijima, J., Masui, R., Kuramitsu, S., Kamiya, N.

    Acta Crystallographica Section D Biological Crystallography   D59 ( 9 )   1610 - 1618   2003.09

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  • Fluorescence resonance energy transfer analysis of protein translocase. SecYE from Thermus thermophilus HB8 forms a constitutive oligomer in membranes Reviewed

    278 ( 16 )   14257 - 14264   2003.04

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  • Overproduction, crystallization and preliminary X-ray diffraction analysis of a quinone oxidoreductase from Thermus thermophilus HB8 Reviewed

    58 ( 8 )   1365 - 1367   2002.08

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  • The crystal structure of exonuclease RecJ bound to Mn2+ ion suggests how its characteristic motifs are involved in exonuclease activity Reviewed

    99 ( 9 )   5908 - 5912   2002.04

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  • Crystal structure of thermostable DNA photolyase: Pyrimidine-dimer recognition mechanism Reviewed

    98 ( 24 )   13560 - 13565   2001.11

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  • Overexpression, purification and characterization of RecJ protein from Thermus thermophilus HB8 and its core domain Reviewed

    29 ( 22 )   4617 - 4624   2001.11

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  • Cloning and overexpression of the oah1 gene encoding O-acetyl-L-homoserine sulfhydrylase of Thermus thermophilus HB8 and characterization of the gene product Reviewed

    1549 ( 1 )   61 - 72   2001.09

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  • Crystallization and preliminary X-ray studies of a thermostable DNA photolyase from Thermus thermophilus HB8 Reviewed

    8 ( 6 )   495 - 498   2001.06

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  • Observation of RecA protein monomer by small angle X-ray scattering with synchrotron radiation Reviewed

    482 ( 42737 )   159 - 162   2000.09

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  • Interaction of UvrA and UvrB proteins with a fluorescent single-stranded DNA. Implication for slow conformational change upon interaction of UvrB with DNA Reviewed

    275 ( 18 )   13235 - 13242   2000.05

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  • Fluorescence and Excitation Escherichia coli RecA protein spectra analyzed separatedly for tyrosine and tryptophan residues Reviewed International coauthorship

    376 ( 1 )   124 - 140   2000.04

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  • The RadA protein from a hyperthermophilic archaeon Pyrobaculum islandicum is a DNA-dependent ATPase that shows two disparate catalytic modes, with a transition termperature at 75 degrees C Reviewed International coauthorship

    267 ( 4 )   1125 - 1137   2000.02

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  • Efficient strand transfer by the RadA recombinase from the hyperthermophilic archaeon Desulfurococcus amylolyticus Reviewed International coauthorship

    182 ( 1 )   130 - 134   2000.01

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  • Crystal structure of Thermus thermophilus HB8 UvrB protein, a key enzyme of nucleotide excision repair Reviewed

    126 ( 6 )   986 - 990   1999.12

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  • Purification, molecular cloning, and catalytic activity of Schizosaccharomyces pombe pyridoxal reductase. A possible additional family in the aldo-keto reductase superfamily Reviewed

    274 ( 33 )   23185 - 23190   1999.08

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  • Crystallization and preliminary X-ray diffraction studies of a DNA excision repair enzyme, UvrB, from Thermus thermophilus HB8 Reviewed

    55 ( 3 )   705 - 705   1999.03

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  • Characterization of the oligomeric states of RecA protein: monomeric RecA protein can form a nucleoprotein filament Reviewed

    Biochemistry   37 ( 42 )   14788 - 14797   1998.10

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  • Domain organization and functional analysis of Thermus thermophilus MutS protein Reviewed

    26 ( 18 )   4153 - 4159   1998.09

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  • Probing of DNA-binding site of Escherichia coli RecA protein by utilizing 1-anilinonaphthalene-8-sulfonic acid Reviewed

    Biochemistry   37 ( 35 )   12133 - 12143   1998.09

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  • Local folding of the N-terminal domain of Escherichia coli RecA controls protein-protein interaction Reviewed

    272 ( 44 )   27707 - 27715   1997.10

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  • Domain structure of Thermus thermophilus UvrB protein. Similarity in domain structure to a helicase Reviewed

    272 ( 36 )   22703 - 22713   1997.09

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  • RecA protein has extremely high cooperativity for substrate in its ATPase activity Reviewed

    123   450 - 457   1997.03

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  • An aspartate aminotransferase from an extremely thermophilic bacterium, Thermus thermophilus HB8 Reviewed

    119 ( 1 )   135 - 144   1996.01

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  • N-terminal 33 amino acid residues of Escherichia coli RecA protien contribute to its self-assembly Reviewed

    J. Mol. Biol.   250 ( 4 )   471 - 483   1995.07

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  • Interaction of RecA protein with ATP and its analogues Reviewed

    116 ( 5 )   960 - 966   1994.11

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  • The presence of free D-serine, D-alanine, and D-proline in human plasma Reviewed

    Experientia   48 ( 3 )   986 - 988   1992.03

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  • Amino acid sequences of ferredoxins from Alocasia macrorrhiza Schott in Papua New Guinea Reviewed

    5 ( 1 )   13 - 19   1992.01

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  • The amino acid sequences of the 9kDa polypeptide and partial amino acid sequence of the 20kDa polypeptide of mitochondrial NADH:ubiquinone oxidoreductase Reviewed International coauthorship

    110 ( 4 )   575 - 582   1991.10

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  • The amino acid sequences of two 13kDa polypeptides and partial amino acid sequence of 30kDa polypeptide of complex I from bovine heart mitochondria: Possible location of iron-sulfur clusters Reviewed International coauthorship

    109 ( 4 )   534 - 543   1991.04

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  • Characterization, amino acid sequence and phylogenetic considerations regarding the ferredoxin from Ochromonas danica Reviewed International coauthorship

    Phytochemistry   27 ( 9 )   2817 - 2820   1988.09

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  • Properties and amino acid sequence of the ferredoxin from the unicellular cyanobacterium Synechococcus 6307 Reviewed International coauthorship

    Phytochemistry   27 ( 9 )   2821 - 2826   1988.09

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  • Properties and structure of the ferredoxin from Synechococcus 6301 (Anacystis nidulans) Reviewed International coauthorship

    Biochemical Journal   252 ( 2 )   571 - 575   1988.06

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Books and Other Publications

  • 生命科学が変わる! タンパク質の構造・機能の基礎から研究テーマ例まで

    倉光成紀、増井良治、中川紀子( Role: Joint author)

    大阪大学出版会  2024.03  ( ISBN:978-4-87259-797-4

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    Total pages:262   Book type:Textbook, survey, introduction Participation form:Second Author

  • Enzymic reaction of proteins

    ( Role: Sole author)

    2009.08 

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    Total pages:171   Responsible for pages:1-27  

  • Atomic biology in post-genome era

    ( Role: Joint author)

    2004.08 

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    Book type:Scholarly book

MISC

  • Acyl-Proteome with Protein Tertiary Structure Information Reveals the Significance of Lysine Acylations Reviewed

    Okanishi Hiroki, Masui Ryoji, Kim Kwang, Kuramitsu Seiki

    Japanese Proteome Society Proteome Letters   3 ( 1 )   15 - 22   2018.07

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    Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Kind of work:Joint Work  

  • Structural Analysis of a Protease-like Protein with DNA-binding Activity Invited Reviewed

    The Crystallographic Society of Japan, Journal of the Crystallographic Society of Japan   60 ( 2 )   74 - 75   2018

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    Publishing type:Rapid communication, short report, research note, etc. (scientific journal)   Kind of work:Single Work  

  • Functional analysis of new proteases from an extremely thermophilic organism, Thermus theromphilus HB8

    Yumi Kimura, Daisuke Sasaki, Naoya Fujimura, Tadashi Ono, Chinami Sako, Suzuka Yamasaki, Ryoji Masui, Noriko Nakagawa

    Protein Science   26   92 - 92   2017

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    Publishing type:Research paper, summary (international conference)   Kind of work:Joint Work  

  • 機能未知タンパク質の機能解明を目指して ヌクレオチド代謝を例に Invited Reviewed

    増井 良治

    (公社)日本生化学会 生化学   87 ( 6 )   749 - 751   2015.12( ISSN:0037-1017

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    Publishing type:Rapid communication, short report, research note, etc. (scientific journal)   Kind of work:Single Work  

    ヌクレオチドや核酸に関わる配列モチーフを持つものの、その機能がはっきりしない蛋白質に着目して構造機能解析を行ってきた。それらの中には重要な生理的機能を担っているものがまだある可能性がある。高度好熱菌の場合を例に、機能未知蛋白質の機能解明を目指した例をppGpp分解酵素Ndx8(TTHA1939)、dNTP分解酵素Tt-dNTPase(TTHA0412)、nanoRNA分解酵素(TTHA0118)に分けて解説した。

  • Discovery of Lys propionylation as one of abundant post-translational modifications

    Okanishi Hiroki, Kim Kwang, Nakagawa Noriko, Kuramitsu Seiki, Masui Ryouji

    Japanese Proteome Society Proteome Letters   2015   183 - 183   2015

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    Publishing type:Research paper, summary (national, other academic conference)   Kind of work:Joint Work  

  • Molecular mechanisms of the whole DNA repair system: A comparison of bacterial and eukaryotic systems Reviewed

    2010   179594   2010.10

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  • In vivo substrate screening for overlooked enzyma activiy Invited Reviewed

    Takushi Ooga, Ryoji Masui

    47 ( 12 )   814   2009.12

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    Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Kind of work:Joint Work  

  • Structural proteomics of metabolism-related proteins Invited

    Tanpakushitsu Kakusan Kouso   53 ( 5 )   604 - 607   2008.04

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    Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Kind of work:Joint Work  

  • 酵素ハンドブック(第3版) Reviewed

    増井 良治

    朝倉書店   2008

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    Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Kind of work:Single Work  

  • タンパク3000の成果とインパクト:個別的解析拠点の研究成果:代謝系 Reviewed

    増井 良治

    蛋白質核酸酵素   53(5), 604-607   2008

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    Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Kind of work:Single Work  

  • 生化学辞典(第4版) Invited Reviewed

    増井 良治

    東京科学同人   2007

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    Publishing type:Article, review, commentary, editorial, etc. (other)   Kind of work:Joint Work  

  • Structural-Biological Whole Cell Project of Thermus thermophilus HB8:From Structural Genomics to Systems Biology

    The Journal of the Crystallographic Society of Japan   48   403 - 410   2006.12

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    Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • MutM: Single C2C2 zinc finger-DNA interaction

    "Zinc Finger Proteins: from Atomic Contact to Cellular Function" (edited by Shiro Iuchi and Natalie Kuldell), Landes Bioscience   2005.02

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    Publishing type:Article, review, commentary, editorial, etc. (other)  

  • Genome sequence analysis of an extreme thermophile Thermus thermophilus HB8

    113 - 128   2004.04

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    Publishing type:Article, review, commentary, editorial, etc. (other)  

  • Mutagenesis of genes by PCR

    2003.10

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    Publishing type:Article, review, commentary, editorial, etc. (other)  

  • Overexpression and preparation of tagged proteins in microorganisms Invited

    114 - 130   2002.10

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    Publishing type:Article, review, commentary, editorial, etc. (other)   Kind of work:Joint Work  

  • Systematic expression of bacterial proteins Invited Reviewed

    Tanpakushitsu Kakusan Kouso   47 ( 8, suppl )   1009 - 1013   2002.08

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  • Repair mechanism of oxidative DNA damages Invited Reviewed

    Tanpakushitsu Kakusan Kouso   46 ( 11 )   1618 - 1624   2001.11

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  • Structure-function relationships of the DNA repair protein UvrB from Thermus thermophilus Invited

    Tanpakushitsu Kakusan Kouso   46 ( 8 )   968 - 975   2001.08

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    Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Kind of work:Joint Work  

  • Structural genomics projects in Japan Reviewed

    7 ( 11, supp )   943 - 945   2000.11

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  • What can we learn from the whole genome sequences of thermopiles Invited Reviewed

    Tanpakushitsu Kakusan Kouso   44 ( 2 )   165 - 170   1999.02

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  • Nucleotide excision repair in prokaryotes Invited

    Jikken Igaku   16 ( 8 )   1069 - 1075   1998.08

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    Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Kind of work:Joint Work  

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Presentations

  • Structural analysis of the non-Hanks-type protein kinase TpkB provides new insights into the function of PrkA/YeaG family Domestic conference

    Masayuki Torii, Kazutoshi Tani, Ryo Kanno, Malgorzata Hall, Akira Mizoguchi, Bruno M. Humbel, Ryoji Masui

    The 96th Annual Meeting of the Janapese Biochemical Society  2023.10 

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    Presentation type:Poster presentation  

  • 高度好熱菌ヌクレオシド一リン酸キナーゼ群のリン酸化による活性調節 Domestic conference

    西脇杏,増井良治

    第45回日本分子生物学会年会  2022.12  日本分子生物学会

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    Presentation type:Poster presentation  

    Venue:幕張メッセ  

  • 高度好熱菌DNA結合タンパク質TkaPの機能と活性調節機構の解析 Domestic conference

    井上葵、高尾和也、井上真男、増井良治

    第22回日本蛋白質科学会年会  2022.06  日本蛋白質科学会

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    Presentation type:Poster presentation  

    Venue:つくば国際会議場  

  • 脂肪酸キナーゼのリン酸基転移反応を担う触媒残基の解析 Domestic conference

    浦野桃花,中原駿也,中谷茉矢,増井良治

    第68回日本生化学会近畿支部例会  2022.05  日本生化学会近畿支部

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    Presentation type:Oral presentation (general)  

    Venue:滋賀医科大学  

  • ⾼度好熱菌ヌクレオシド⼀リン酸キナーゼ群のリン酸化による活性調節 Domestic conference

    西脇杏,増井良治

    第68回日本生化学会近畿支部例会  2022.05  日本生化学会近畿支部

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    Venue:滋賀医科大学  

  • Structural and functional characterization of a bacterial protein kinase with the P-loop motif Domestic conference

    2021.12 

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  • Structural and functional analysis of the bacterial small Zn-finger protein Domestic conference

    2021.12 

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  • In vivo analysis of functional regions within a novel RHH-type DNA-binding protein Domestic conference

    2021.12 

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  • Involvement of the C-terminal helix of a novel RHH-type DNA-binding protein in phosphorylation-dependent regulatio Domestic conference

    2021.11 

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  • バクテリア由来small Zn-finger proteinの機能・構造解析 Domestic conference

    栗波紗生, 福井健二, 村川武志, 矢野貴人, 増井良治

    第67回日本生化学会近畿支部例会  2021.05 

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  • Biochemical characterization of a small zinc-finger protein from Thermus thermophilus Domestic conference

    Saki Kurinami,Ryoji Masui

    The 43rd Annual Meeting of the Molecular Biology Society of Japan  2020.12 

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  • Phosphoregulation of a Thermus ribbon-helix-helix-type DNA-binding protein requires a conformational change in the negatively charged C-terminal helix Domestic conference

    Aoi Inoue,Kazuya Takao,Kenji Fukui,Masao Inoue,Yuki Fujii,Takato Yano,Ryoji Masui

    The 43rd Annual Meeting of the Molecular Biology Society of Japan  2020.12 

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  • Genetic and biochemical characterization of a bacterial protein kinase with the Walker A motif Domestic conference

    Masayuki Torii, Yota Iio, Suzuka Yamasaki, Ryoji Masui

    The 43rd Annual Meeting of the Molecular Biology Society of Japan  2020.12 

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  • Structural change of a thermophilic protein kinase induced by ATP binding Domestic conference

    Masayuki Torii, Yusuke Fujino, Masao Inoue, Yuki Fujii, Hiroki Okanishi, Yoshikatsu Kanai, Ryoji Masui

    The 21 st Annual Meeting of the Japanese Society for Extremophiles  2020.11 

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  • Structural basis for substrate recognition by cytidylate kinase Domestic conference

    Ryosuke Mega, Noriko Nakagawa, Seiki Kuramitsu. Ryoji Masui

    The 42nd Annual Meeting of the Molecular Biology Society of Japan  2019.12 

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  • Analysis of functionally unknown proteins related to nucleic acid transaction Domestic conference

    Kenji Fukui, Yuki Fujii, Taisuke Wakamatsu, Ryoji Masui

    第42回 日本分子生物学会年会  2019.12 

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    Presentation type:Symposium, workshop panel (nominated)  

  • Structural and functional domain analysis of fatty acid kinase Domestic conference

    Maya Nakatani, Taihei Murakami, Hiroki Okanishi, Makoto Miyata, Ryoji Masui

    The 42nd Annual Meeting of the Moleular Bioloy Society of Japan  2019.12 

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  • NurA and HerA in bacteria contribute to the repair of UV-induced lesions with recombination mediator proteins Domestic conference

    Yuki Fujii, Masao Inoue, Kenji Fukui, Ryoji Masui

    The 42nd Annual Meeting of the Moleular Bioloy Society of Japan  2019.12 

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  • Analysis of functionally unknown proteins related to nucleic acid transaction Domestic conference

    Kenji Fukui, Yuki Fujii, Taisuke Wakamatsu, Ryoji Masui

    第42回 日本分子生物学会年会  2019.12 

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  • Structural and functional domain analysis of fatty acid kinase Domestic conference

    Maya Nakatani, Taihei Murakami, Hiroki Okanishi, Makoto Miyata, Ryoji Masui

    The 42nd Annual Meeting of the Moleular Bioloy Society of Japan  2019.12 

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  • NurA and HerA in bacteria contribute to the repair of UV-induced lesions with recombination mediator proteins Domestic conference

    Yuki Fujii, Masao Inoue, Kenji Fukui, Ryoji Masui

    The 42nd Annual Meeting of the Moleular Bioloy Society of Japan  2019.12 

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  • Mechanism of drastic stabilization by ATP of a thermophilic protein kinase structure Domestic conference

    Yusuke Fujino, Masao Inoue, Yuki Fujii, Ryoji Masui

    The 20th Annual Meeting of the Japanese Society for Extremophiles  2019.11 

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  • Structural basis for ligand binding to CMP kinase revealed by X-ray crystallographic analysis of the different liganded forms Domestic conference

    Ryosuke Mega, Noriko Nakagawa, Seiki Kuramitsu, Ryoji Masui

    The 92nd Annual Meeting of the Japanese Biochemical Society  2019.09 

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  • Structural and functional analysis of fatty acid kinase of Thermus thermophilus HB8 International conference

    Maya Nakatani, Taihei Murakami,, Hiroki Okanishi,, Norie Araki, Makoto Miyata, Ryoji Masui

    10th OCARINA International Symposium  2019.03 

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  • Analysis of functionally unknown proteins in Thermus thermophilus Domestic conference

    Ryoji Masui

    第41回日本分子生物学会年会  2018.11 

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  • 高度好熱菌脂肪酸キナーゼの構造機能解析 Domestic conference

    中谷 茉矢, 村上 泰平, 岡西 広樹, 荒木 令江, 増井 良治

    第91回日本生化学会大会  2018.09  (公社)日本生化学会

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  • 高度好熱菌脂肪酸キナーゼの構造機能解析 Domestic conference

    中谷茉矢,村上泰平,岡西広樹,荒木令江,増井良治

    第65回日本生化学会近畿支部例会  2018.05 

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  • DNA-binding activity of the multifunctional enzyme, nucleoside diphosphate kinase International conference

    Chinami Sako, Masaaki Miyamoto, Satoshi Akanuma, Ryoji Masui

    9th OCARINA International Symposium  2018.03 

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  • 高度好熱菌 protein kinase TpkD の構造安定性に対する ATP の効果 Domestic conference

    藤野 友輔, 井上 真男, 藤井 裕己, 倉光 成紀, 増井 良治

    生命科学系学会合同年次大会  2017.12  生命科学系学会合同年次大会運営事務局

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  • 多機能酵素ヌクレオシドニリン酸キナーゼのDNA結合活性 Domestic conference

    佐古 千菜美, 宮本 昌明, 赤沼 哲史, 増井 良治

    生命科学系学会合同年次大会  2017.12  生命科学系学会合同年次大会運営事務局

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  • リン酸基転移酵素にみられる基質特異性の広がりと新たな機能獲得 Domestic conference

    増井 良治

    生命科学系学会合同年次大会  2017.12  生命科学系学会合同年次大会運営事務局

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  • リン酸化を介してDNA結合能が制御される新奇なDNA結合タンパク質の立体構造解析 Domestic conference

    高尾 和也, 飯尾 洋太, 井上 真男, 藤井 裕己, 倉光 成紀, 増井 良治

    生命科学系学会合同年次大会  2017.12  生命科学系学会合同年次大会運営事務局

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  • DNA修復酵素RadAのLonプロテアーゼ様ドメインはDNA結合活性を持つ Domestic conference

    井上 真男, 福井 健二, 藤井 裕己, 中川 紀子, 矢野 貴人, 倉光 成紀, 増井 良治

    生命科学系学会合同年次大会  2017.12  生命科学系学会合同年次大会運営事務局

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  • 多機能酵素ヌクレオシドニリン酸キナーゼのDNA結合活性 Domestic conference

    佐古 千菜美, 宮本 昌明, 赤沼 哲史, 増井 良治

    生命科学系学会合同年次大会  2017.12  生命科学系学会合同年次大会運営事務局

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  • Functional analysis of new proteases from an extremely thermophilic organism, Thermus thermophilus HB8 International conference

    Yumi Kimura, Daisuke Sasaki, Naoya Fujimura, Tadashi Ono, Chinami Sako, Suzuka Yamasaki, Ryoji Masui, Noriko Nakagawa

    The 31st Annual Symposium of The Protein Society  2017.07 

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  • 翻訳後修飾を受ける機能未知DNA結合タンパク質の構造機能解析 Domestic conference

    高尾和也,飯尾洋太,井上真男,,藤井裕己,倉光成紀,増井良治

    酵素補酵素研究会2017  2017.06 

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  • 高度好熱菌protein kinase TpkDの構造安定性に対するATPの効果 Domestic conference

    藤野友輔, 井上真男, 藤井裕己, 倉光成紀, 増井良治

    酵素補酵素研究会2017  2017.06 

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  • DNA 修復酵素 RadA の Lon プロテアーゼ様ドメインは DNA 結合活性を持つ Domestic conference

    井上真男, 福井健二, 藤井裕己, 中川紀子, 矢野貴人, 倉光成紀, 増井良治

    酵素補酵素研究会2017  2017.06 

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  • Effects of ATP on structural stability of protein kinase TpkD from Thermus thermophilus HB8 Domestic conference

    Yusuke Fujino, Masao Inoue,, Yuki Fujii, Seiki Kuramitsu, Ryoji Masui

    第17回日本蛋白質科学会年会  2017.06 

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  • Structural and functional analysis of a novel DNA-binding protein TkaP from Thermus thermophilus HB8 Domestic conference

    Kazuya Takao, Yota Iio, Masao Inoue, Yuki Fujii, Seiki Kuramitsu, Ryoji Masui

    第17回日本蛋白質科学会年会  2017.06 

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  • 多機能酵素ヌクレオシド二リン酸キナーゼのDNA結合活性 Domestic conference

    佐古千菜美,宮本昌明,赤沼哲史,増井良治

    第64回日本生化学会近畿支部例会  2017.05 

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  • Structural and functional analysis of a novel DNA-binding protein TkaP from Thermus thermophilus HB8 International conference

    Kazuya Takao, Yota Iio, Masao Inoue, Yuki Fujii, Yu-hei Tahara, Makoto Miyata, Seiki Kuramitsu, Ryoji Masui

    8th OCARINA International Symposium  2017.03 

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  • NurA and HerA of the Extremely Thermophilic Eubacterium Thermus thermophilus HB8 International conference

    Yuki Fujii, Masao Inoue, Kenji Fukui, Kwang Kim, Seiki Kuramitsu, Ryoji Masui

    Extremophiles 2016  2016.09 

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  • 高度好熱菌Thermus thermophilus HB8由来の新奇なDNA結合タンパク質(TkaP)の構造・機能的解析 Domestic conference

    高尾 和也, 飯尾 洋太, 井上 真男, 藤井 裕己, 藤野 友輔, 倉光 成紀, 増井 良治

    第89回日本生化学会大会  2016.09  (公社)日本生化学会

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  • 高度好熱菌protein kinase TpkDの構造安定性に対するリガンドの効果 Domestic conference

    藤野 友輔, 宮川 武朗, 井上 真男, 藤井 裕己, 倉光 成紀, 増井 良治

    第89回日本生化学会大会  2016.09  (公社)日本生化学会

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  • Post-translational acylations occurring in a wide range of organisms: lysine acetylation, propionylation, and succinylation Domestic conference

    Okanishi Hiroki, Kobayashi Daiki, Araki Norie, Masui Ryoji, Kuramitsu Seiki

    2016 Annual Meeting of Japanese Proteomics Society  2016.07 

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    DOI: 10.14889/jhupo.2016.0.55.0

  • DNA 相同組換えに関わる RecA パラログタンパク質 RadA/Sms が持つ Lon プロテアーゼ様ドメインの構造と機能 Domestic conference

    井上真男, 福井健二, 藤井裕己, 矢野貴人, 倉光成紀, 増井良治

    第16回日本蛋白質科学会年会  2016.06 

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  • バクテリア由来アーキア型ヌクレアーゼNurAとヘリカーゼHerAの細胞機能解析 Domestic conference

    藤井 裕己, 井上 真男, 福井 健二, 金 光, 倉光 成紀, 上田 昌宏, 増井 良治

    日本生化学会大会・日本分子生物学会年会合同大会講演要旨集  2015.12  (公社)日本生化学会

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  • RecA様タンパク質RadA/Sms C末端ドメインの構造と機能 Domestic conference

    井上 真男, 福井 健二, 藤井 裕己, 矢野 貴人, 倉光 成紀, 増井 良治

    日本生化学会大会・日本分子生物学会年会合同大会講演要旨集  2015.12  (公社)日本生化学会

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  • バクテリアにおける翻訳後修飾 高度好熱菌におけるアシル化プロテオーム解析 Domestic conference

    岡西 広樹, 金 光, 増井 良治, 倉光 成紀

    日本生化学会大会プログラム・講演要旨集  2014.10  (公社)日本生化学会

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  • ウリジンシチジンキナーゼ中のウリジンのプロトン化状態についての理論的研究 Domestic conference

    田中 弥, 氏家 謙, 友池 史明, 庄司 光男, 栢沼 愛, 増井 良治, 倉光 成紀, 重田 育照

    日本生化学会大会プログラム・講演要旨集  2014.10  (公社)日本生化学会

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  • ウリジンシチジンキナーゼの基質結合相互作用についての理論的研究(A theoretical study on the substrate bindings in uridine-cytidine kinase) Domestic conference

    Tanaka Wataru, Ujiie Yuzuru, Tomoike Fumiaki, Shoji Mitsuo, Kayanuma Megumi, Masui Ryoji, Kuramitsu Seiki, Shigeta Yasuteru

    生物物理  2014.08  (一社)日本生物物理学会

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  • 耐熱性ミスマッチDNA修復蛋白質を利用したPCR法の改良 Domestic conference

    福井 健二, 島田 敦広, 豊竹 洋佑, 増井 良治, 倉光 成紀

    日本生化学会大会プログラム・講演要旨集  2013.09  (公社)日本生化学会

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  • 短鎖DNA/RNA特異的エキソヌクレアーゼの立体構造と反応機構 Domestic conference

    植村 有里, 若松 泰介, 中川 紀子, Hunt John Francis, Montelione Gaetano Thomas, 倉光 成紀, 増井 良治

    日本生化学会大会プログラム・講演要旨集  2013.09  (公社)日本生化学会

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  • 少数のアミノ酸残基によって決定されるプリンヌクレオシドホスホリラーゼの基質特異性 Domestic conference

    友池 史明, 倉光 成紀, 増井 良治

    日本生化学会大会プログラム・講演要旨集  2013.09  (公社)日本生化学会

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  • 高度好熱菌丸ごと一匹解析 異なるファミリーに属するプロテインキナーゼは異なる耐熱化機構を持つ Domestic conference

    宮川 武朗, 飯尾 洋太, 増井 良治, 倉光 成紀

    日本生化学会大会プログラム・講演要旨集  2012.12  (公社)日本生化学会

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  • 短鎖DNA/RNA特異的エキソヌクレアーゼの反応機構 Domestic conference

    植村 有里, 若松 泰介, Hunt John, Montelione Gaetano, 中川 紀子, 増井 良治, 倉光 成紀

    日本生化学会大会プログラム・講演要旨集  2012.12  (公社)日本生化学会

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  • 単一のアミノ酸残基によって決定されるuridine-cytidine kinaseの基質特異性 Domestic conference

    友池 史明, 中川 紀子, 増井 良治, 倉光 成紀

    日本生化学会大会プログラム・講演要旨集  2012.12  (公社)日本生化学会

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  • Thermus thermophilus HB8の全細胞解析 RNaseによるmRNA分解の調節(Whole cell analysis of Thermus thermophilus HB8: The regulation of mRNA decay by RNases) Domestic conference

    Ohyama Hiromasa, Agari Yoshihiro, Fukui Kenji, Nakagawa Noriko, Shinkai Akeo, Masui Ryoji, Kuramitsu Seiki

    日本生化学会大会プログラム・講演要旨集  2012.12  (公社)日本生化学会

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  • RecA様蛋白質RadA/Smsの分子機能解析 Domestic conference

    井上 真男, 福井 健二, 中川 紀子, 増井 良治, 倉光 成紀

    日本生化学会大会プログラム・講演要旨集  2012.12  (公社)日本生化学会

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  • DNAミスマッチ修復タンパク質MutS-MutL間相互作用の動態解析 Domestic conference

    秦 義人, 島田 敦広, 増井 良治, 福井 健二, 倉光 成紀

    日本生化学会大会プログラム・講演要旨集  2012.12  (公社)日本生化学会

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  • DNAポリメラーゼXが持つPHPドメインの塩基除去修復系における役割 Domestic conference

    中根 修平, 中川 紀子, 倉光 成紀, 増井 良治

    日本生化学会大会プログラム・講演要旨集  2012.12  (公社)日本生化学会

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  • ATP加水分解にともなうMutSによるMutLの活性化 Domestic conference

    島田 敦広, 増井 良治, 倉光 成紀, 福井 健二

    日本生化学会大会プログラム・講演要旨集  2012.12  (公社)日本生化学会

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  • 単一のアミノ酸残基によって決定されるuridine-cytidine kinaseの基質特異性 Domestic conference

    友池 史明, 中川 紀子, 増井 良治, 倉光 成紀

    日本生化学会大会プログラム・講演要旨集  2012.12  (公社)日本生化学会

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  • 短鎖DNA/RNA特異的エキソヌクレアーゼの反応機構 Domestic conference

    植村 有里, 若松 泰介, Hunt John, Montelione Gaetano, 中川 紀子, 増井 良治, 倉光 成紀

    日本生化学会大会プログラム・講演要旨集  2012.12  (公社)日本生化学会

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  • ヒトタイプMutLにおけるATP依存的構造機能変化 立体構造予測を利用した構造機能解析 Domestic conference

    飯野 均, 金 光, 山本 竜也, 島田 敦広, 増井 良治, 倉光 成紀, 福井 健二

    日本生化学会大会・日本分子生物学会年会合同大会講演要旨集  2010.12  (公社)日本生化学会

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Outline of education staff

  • 全学共通教育 (全学部対象) では,生物と環境とのかかわりについて学生自身が課題を設定して,調べ,発表する形式の授業を行っている。 また,生物学実験を分担している。学部教育 (理学部対象) では,炭水化物や脂肪,蛋白質といった食物分子からどのようにしてエネルギーを得ているのか,その概要を分子レベルで説明できることを目標としている。また,専門生物学実験を分担している。大学院教育(理学研究科対象)では,生体内の代謝反応を担う酵素タンパク質の構造と機能を実際に研究するための主要な解析方法について説明できるようにすることを目指している。

Charge of on-campus class subject

  • 生物学前期特別研究Ⅱ

    2023   Intensive lecture   Graduate school

  • 後期特別研究

    2023   Intensive lecture   Graduate school

  • 生物学特別研究2A

    2023   Intensive lecture   Graduate school

  • 生物学特別研究1A

    2023   Intensive lecture   Graduate school

  • 海外特別研究2

    2023   Intensive lecture   Graduate school

  • 海外特別研究1

    2023   Intensive lecture   Graduate school

  • 機能解析生物学特別講義B

    2023   Intensive lecture   Graduate school

  • 機能解析生物学演習2A

    2023   Weekly class   Graduate school

  • 機能解析生物学演習1A

    2023   Weekly class   Graduate school

  • 機能解析生物学特論A

    2023   Weekly class   Graduate school

  • 機能解析生物学特論A

    2023   Weekly class   Graduate school

  • 生物学の潮流

    2023   Intensive lecture   Undergraduate

  • 生物学実験A

    2023   Practical Training   Undergraduate

  • 生物学特別研究4A

    2023   Intensive lecture   Graduate school

  • 生物学特別研究3A

    2023   Intensive lecture   Graduate school

  • 海外特別研究4

    2023   Intensive lecture   Graduate school

  • 海外特別研究3

    2023   Intensive lecture   Graduate school

  • 機能解析生物学特別演習

    2023   Intensive lecture   Graduate school

  • 機能解析生物学ゼミナール

    2023   Intensive lecture   Graduate school

  • 後期海外特別研究2

    2023   Intensive lecture   Graduate school

  • 後期特別研究

    2023   Intensive lecture   Graduate school

  • 後期特別研究

    2023   Intensive lecture   Graduate school

  • 生物分子機能学ゼミナール

    2023   Intensive lecture   Graduate school

  • 海外特別研究

    2023   Intensive lecture   Undergraduate

  • 生物学実験S

    2023   Practical Training   Undergraduate

  • 特別研究

    2023   Intensive lecture   Undergraduate

  • 専門生物学演習

    2023   Intensive lecture   Undergraduate

  • 代謝生化学2

    2023   Weekly class   Undergraduate

  • 生物学実験B

    2023   Practical Training   Undergraduate

  • 生命と環境

    2023   Weekly class   Undergraduate

  • 生物学特別研究4B

    2023   Intensive lecture   Graduate school

  • 前期海外特別研究1

    2023   Intensive lecture   Graduate school

  • 生物学前期特別研究Ⅱ

    2023   Intensive lecture   Graduate school

  • 生物学特別研究2B

    2023   Intensive lecture   Graduate school

  • 生物学特別研究1B

    2023   Intensive lecture   Graduate school

  • 機能解析生物学演習2B

    2023   Weekly class   Graduate school

  • 機能解析生物学演習1B

    2023   Weekly class   Graduate school

  • 後期特別研究

    2023   Intensive lecture   Graduate school

  • 専門生物学実験B

    2023   Practical Training   Undergraduate

  • 生物学特別研究3B

    2023   Intensive lecture   Graduate school

  • 生物学特別研究1A

    2022   Intensive lecture   Graduate school

  • 機能解析生物学演習1A

    2022   Intensive lecture   Graduate school

  • 機能解析生物学特論A

    2022   Weekly class   Graduate school

  • 生物学特別研究1B (杉本)

    2022   Intensive lecture   Graduate school

  • 機能解析生物学演習1B (中百舌鳥)

    2022   Weekly class   Graduate school

  • 機能解析生物学演習1B (杉本)

    2022   Intensive lecture   Graduate school

  • 生物学の潮流

    2022   Weekly class   Undergraduate

  • 生物学実験S

    2022   Intensive lecture   Undergraduate

  • 生物学実験A

    2022   Weekly class   Undergraduate

  • 前期特別研究(M2生物学分野)

    2022   Intensive lecture   Graduate school

  • 代謝生化学

    2022   Weekly class   Undergraduate

  • 専門生物学実験B

    2022   Weekly class   Undergraduate

  • 専門生物学演習

    2022   Intensive lecture   Undergraduate

  • 生命と環境/杉本[全]S

    2022   Weekly class   Graduate school

  • 生物学実験B/必:理<生>選:<生以外>(市:時間割表参照)S

    2022   Weekly class   Graduate school

  • 前期特別研究

    2021     Graduate school

  • 専門生物学実験B

    2021     Undergraduate

  • 特別研究

    2021     Undergraduate

  • 生物学実験A

    2021     Undergraduate

  • 生物学実験S

    2021     Undergraduate

  • 生物学の潮流

    2021     Undergraduate

  • 生体分子機能学演習

    2021     Graduate school

  • 代謝調節機能学特論

    2021     Graduate school

  • 代謝生化学

    2021     Undergraduate

  • 専門生物学演習

    2021     Undergraduate

  • 生命と環境

    2021     Undergraduate

  • 専門生物学実験B

    2020     Undergraduate

  • 特別研究

    2020     Undergraduate

  • 生物学実験A

    2020     Undergraduate

  • 生物学実験S

    2020     Undergraduate

  • 生物学の潮流

    2020     Undergraduate

  • 生体分子機能学演習

    2020     Graduate school

  • 代謝調節機能学特論

    2020     Graduate school

  • 代謝生化学

    2020     Undergraduate

  • 専門生物学演習

    2020     Undergraduate

  • 生命と環境

    2020     Undergraduate

  • 前期特別研究

    2020     Graduate school

  • 生命と環境

    2019     Undergraduate

  • 代謝調節機能学特論

    2019     Graduate school

  • 生体分子機能学演習

    2019     Graduate school

  • 専門生物学実験B

    2019     Undergraduate

  • 特別研究

    2019     Undergraduate

  • 生物学実験A

    2019     Undergraduate

  • 生物学実験S

    2019     Undergraduate

  • 生物学の潮流

    2019     Undergraduate

  • 代謝生化学

    2019     Undergraduate

  • 専門生物学演習

    2019     Undergraduate

  • 前期特別研究

    2019     Graduate school

  • 専門生物学演習

    2018     Undergraduate

  • 前期特別研究

    2018     Graduate school

  • 生物学の潮流

    2018     Undergraduate

  • 生物学実験S

    2018     Undergraduate

  • 現代科学と人間

    2018     Undergraduate

  • 生物学実験A

    2018     Undergraduate

  • 特別研究

    2018     Undergraduate

  • 専門生物学実験B

    2018     Undergraduate

  • 生体分子機能学演習

    2018     Graduate school

  • 代謝調節機能学特論

    2018     Graduate school

  • 生命と環境

    2018     Undergraduate

  • 代謝生化学

    2018     Undergraduate

  • 前期特別研究

    2017     Graduate school

  • 生物学実験A

    2017     Undergraduate

  • 生体分子機能学演習

    2017     Graduate school

  • 代謝調節機能学特論

    2017     Graduate school

  • 生物学実験S

    2017     Undergraduate

  • 特別研究

    2017     Undergraduate

  • 専門生物学演習

    2017     Undergraduate

  • 専門生物学実験B

    2017     Undergraduate

  • 現代科学と人間

    2017     Undergraduate

  • 代謝生化学

    2017     Undergraduate

  • 生物学の潮流

    2017     Undergraduate

  • 生体分子機能学演習

    2016     Graduate school

  • 代謝調節機能学特論

    2016     Graduate school

  • 生物学実験S

    2016     Undergraduate

  • 特別研究

    2016     Undergraduate

  • 専門生物学演習

    2016     Undergraduate

  • 専門生物学実験B

    2016     Undergraduate

  • 現代科学と人間

    2016     Undergraduate

  • 代謝生化学

    2016     Undergraduate

  • 生物学の潮流

    2016     Undergraduate

  • 生物学実験S

    2015     Undergraduate

  • 特別研究

    2015     Undergraduate

  • 専門生物学演習

    2015     Undergraduate

  • 専門生物学実験B

    2015     Undergraduate

  • 代謝生化学

    2015     Undergraduate

  • 現代科学と人間

    2015     Undergraduate

  • 生物学の潮流

    2015     Undergraduate

▼display all

Faculty development activities

  • 教育方法の改善  2023

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    【教育方法の改善】専門教育科目「代謝生化学2」において,学生自身が課題を調査し,その結果をスライドにまとめて Moodle に提出し、毎回の授業で代表して数名が発表するというアクティブラーニング形式での授業を実施した。

  • 教育方法の改善  2022

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    【教育方法の改善】大学院科目「機能解析生物学特論A」において、受講学生の研究テーマを題材にして種々の解析手法の解説を行うことにより、学生の主体的な取り組みを促した。

  • 教育方法の改善  2021

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    【教育方法の改善】総合教育科目「生命と環境」において,学生たち自身がグループミーティングを通じて課題テーマを設定し,自分で調査した内容をスライドにまとめ,互いのプレゼンテーションを視聴して意見を述べ合うというアクティブラーニング形式での授業を実施した。

  • 教育方法の改善  2021

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    【教育方法の改善】専門教育科目「代謝生化学」において,WebClass を利用して学生からの質問を集め,それに対する回答を次回の授業の冒頭に行うことにより,双方向のコミュニケーションをとるようにした。

  • 教育方法の改善  2020

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    【教育方法の改善】専門教育科目「代謝生化学」において,学生からの質問に対する回答を音声付きのスライドにまとめて視聴できるようにすることで,授業時間の枠にとらわれずに十分な説明を行うことが可能になった。

  • 教育方法の改善  2020

     More details

    【教育方法の改善】専門教育科目「代謝生化学」において,通常の講義と並行して,学生自身が課題を調査し,その結果を音声付きのスライドにまとめて,オンラインで互いのプレゼンテーションを視聴するというアクティブラーニング形式での授業を実施した。

  • 教育方法の改善  2020

     More details

    【教育方法の改善】総合教育科目「生命と環境」において,学生自身がテーマを設定して,自分で調査し,その内容を音声付きのスライドにまとめ,互いのプレゼンテーションを視聴して意見を述べ合うというアクティブラーニング形式での授業をオンライン形式で実施した。

  • 教育方法の改善  2019

     More details

    総合教育科目「生命と環境」において,学生自身がテーマを設定して,自分で調査し,その内容をスライドにまとめ,グループ内で議論したのち,口頭発表するというアクティブラーニング形式での授業を実施した。

  • FD活動への貢献  2019

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    大学の FD 研修会および理学研究科の FD 研修会に参加し,アクティブラーニングの進め方についての知見を深めた。

  • FD活動への貢献  2018

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    大学の FD 研修会および理学研究科の FD 研修会に参加し,アクティブラーニングの進め方についての知見を深めた。

  • FD活動への貢献  2017

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    大学の FD 研修会および理学研究科の FD 研修会に参加し,アクティブラーニングの進め方についての知見を深めた。

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Number of papers published by graduate students

  • 2023

    Number of graduate students presentations:0

  • 2022

    Number of graduate students presentations:1

  • 2020

    Number of graduate students presentations:1

Original item・Special report (Education Activity)

  • 2023

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    Original item:生物学専攻・前期博士課程2年生の担任をつとめた。

  • 2021

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    Original item:2018年度入学の生物学科4年生の担任をつとめた。

  • 2020

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    Original item:2018年度入学の生物学科3年生の担任をつとめた。

  • 2019

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    Original item:2018年度入学の生物学科2年生の担任をつとめた。

  • 2018

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    Original item:2018年度入学の生物学科1年生の担任をつとめた。

  • 2017

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    Original item:博士前期課程(生物分野)の担任をつとめた。

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Social Activities

  • 大阪市立大学 公開講座 文化交流センター講座

    Role(s): Lecturer

    大阪市立大学文化交流センター  大阪市立大学 文化交流センター  2017.12

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    Audience: General public

    Type:Lecture

    Number of participants:30(人)

    「好熱菌から生命の起源と未来を考える」というタイトルで,生命誕生の謎や生命とは何かについて考えてみるという内容の講義を行った。

Job title

  • Job title within the department

    School of Science Department of Biology 

    学科長  2024.04

  • Job title within the department

    Graduate School of Science Department of Biology 

    専攻長  2024.04