Updated on 2024/03/16
Graduate School of Engineering Division of Science and Engineering for Materials, Chemistry and Biology
Associate Professor 2022.04 - Now
School of Engineering Department of Chemistry and Bioengineering
Associate Professor 2022.04 - Now
博士(工学) （ Osaka City University ）
Life Science / Functional biochemistry / Protein Engineering
自己会合ペプチドを利用したタンパク質の高機能化
自己会合ペプチド、組換え抗体、分子設計 Individual
1900.04
医薬応用を目指した高機能人工抗体の開発
組換え抗体、抗体医薬、がん Individual
1900.04
Protein Science Society of Japan
Domestic
Osaka City University Doctor's Course
- 2003
Osaka City University Graduated/Completed
- 1998
Cancer therapeutic trispecific antibodies recruiting both T and natural killer cells to cancer cells Reviewed
Kouki Kimura, Atsushi Kuwahara, Saori Suzuki, Takeshi Nakanishi, Izumi Kumagai, and Ryutaro Asano
Oncology Reports 50 ( 6 ) 212 2023.10（ ISSN:1021335X ）
Risa Yamada, Ishin Nakahara, Izumi Kumagai, Ryutaro Asano, Takeshi Nakanishi, and Koki Makabe
Scientific Reports 13 15961 2023.09
Generation of rat monoclonal antibodies against human epidermal growth factor receptor 2 Reviewed
Honoka Yamamoto, Takeshi Nakanishi, Kan-Ichiro Ihara, Taro Tachibana, and Chikako Yokoyama
Monoclon. Antib. Immunodiagn. Immunother. 42 ( 2 ) 59 - 64 2023.03
Build-up functionalization of anti-EGFR x anti-CD3 bispecific diabodies by integrating high-affinity mutants and functional molecular formats Reviewed
Asano Ryutaro, Hosokawa Katsuhiro, Taki Shintaro, Konno Shota, Shimomura Ippei, Ogata Hiromi, Okada Mai, Arai Kyoko, Onitsuka Masayoshi, Omasa Takeshi, Nakanishi Takeshi, Umetsu Mitsuo, Kumagai Izumi
SCIENTIFIC REPORTS 10 ( 1 ) 2020.03（ ISSN:2045-2322 ）
Construction of a circularly connected VHH bispecific antibody (cyclobody) for the desirable positioning of antigen-binding sites Reviewed
Hemmi Saki, Asano Ryutaro, Kimura Kouki, Umetsu Mitsuo, Nakanishi Takeshi, Kumagai Izumi, Makabe Koki
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 523 ( 1 ) 72 - 77 2020.02（ ISSN:0006-291X ）
Chemically Crosslinked Bispecific Antibodies for Cancer Therapy: Breaking from the Structural Restrictions of the Genetic Fusion Approach Reviewed
Ueda Asami, Umetsu Mitsuo, Nakanishi Takeshi, Hashikami Kentaro, Nakazawa Hikaru, Hattori Shuhei, Asano Ryutaro, Kumagai Izumi
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21 ( 3 ) 2020.02
Functional domain order of an anti-EGFR × anti-CD16 bispecific diabody involving NK cell activation Reviewed
Atsushi Kuwahara, Keisuke Nagai, Takeshi Nakanishi, Izumi Kumagai, and Ryutaro Asano
Int. J. Mol. Sci. 21 8914 2020
Generation of the rat monoclonal antibody against the extracellular domain of human CD63 by DNA immunization Reviewed
Liu Xu, Kan-ichiro Ihara, Saori Yoshimura, Daijiro Konno, Akira Tachibana, Takeshi Nakanishi, and Taro Tachibana
Monoclon. Antib. Immunodiagn. Immunother. 39 74 - 76 2020
Affinity maturation of humanized anti-epidermal growth factor receptor antibody using a modified phage-based open sandwich selection method. Reviewed
Sanada Hideaki, Kobayashi Kazuki, Oyama Kenji, Maru Takamitsu, Nakanishi Takeshi, Umetsu Mitsuo, Asano Ryutaro, Kumagai Izumi
Scientific Reports 8 5414 2018.04（ ISSN:2045-2322 ）
Soluble expression in Escherichia coli of a single-domain antibody–tumor necrosis factor α fusion protein specific for epidermal growth factor receptor. Reviewed
Tomohiro Osaki, Takeshi Nakanishi, Motoshi Aoki, Takahiro Omizu, Daisuke Nishiura, Masaya Kitamura
Monoclon. Antib. Immunodiagn. Immunother. 37 ( 1 ) 20 - 25 2018.02
Photoinduced electron transfer from aromatic amino acids to the excited isoalloxazine in single mutated flavin mononucleotide binding proteins: Effect of the dimer formation on the rate and the electrostatic energy inside the proteins. Reviewed International coauthorship
Nadtanet Nunthaboot, Kiattisak Lugsanangarm, Arthit Nueangaudom, Somsak Pianwanit, Sirirat Kokpol, Fumio Tanaka, Seiji Taniguchi, Haik Chosrowjan, Takeshi Nakanishi, Masaya Kitamura
Comput. Theor. Chem. 1108 1 - 9 2017
Conformational difference between two subunits in flavin mononucleotide binding protein dimers from Desulfovibrio vulgaris (MF): Molecular dynamics simulation. Reviewed
N. Nunthaboot, K. Lugsanangarm, S. Pianwanit, S. Kokpol, F. Tanaka, T. Nakanishi, M. Kitamura
Comput. Biol. Chem. 64 113 - 125 2016.10
Photoinduced electron transfer from aromatic amino acids to the excited isoalloxazine in flavin mononucleotide binding protein. Is the rate in the inverted region of donor – acceptor distance not real? Reviewed
N. Nunthaboot, K. Lugsanangarm, A. Pianwanit, F. Tanaka, S. Taniguchi, H. Chosrowjan, T. Nakanishi, M. Kitamura et al.
J. Photochem. Photobiol. A: Chem. 326 60 - 68 2016.07
Generation and characterization of rat monoclonal antibodies against epidermal growth factor receptor. Reviewed
T. Osaki, C.X. Wang, T. Tachibana, M. Azuma, M. Kitamura, T. Nakanishi
Monoclon. Antib. Immunodiagn. Immunother. 34 ( 6 ) 418 - 422 2015.12
Development of a bispecific antibody tetramerized through hetero-associating peptides. Reviewed
T. Osaki, S. Fujisawa, M. Kitaguchi, M. Kitamura, T. Nakanishi
FEBS J. 282 ( 22 ) 4389 - 4401 2015.11
Bell-shaped dependence of the rate of ultrafast photoinduced electron transfer from aromatic amino acids to the excited flavin on the donor-acceptor distance in FMN binding proteins Reviewed
Nunthaboot Nadtanet, Lugsanangarm Kiattisak, Pianwanit Somsak, Kokpol Sirirat, Tanaka Fumio, Taniguchi Seiji, Chosrowjan Haik, Nakanishi Takeshi, Kitamura Masaya
COMPUTATIONAL AND THEORETICAL CHEMISTRY 1030 9 - 16 2014.02（ ISSN:2210-271X ）
Mechanism of photoinduced electron transfer from tyrosine to the excited flavin in the flavodoxin from Helicobacter pylori. A comparative study with the flavodoxin and flavin mononucleotide binding protein from Desulfovibrio vulgaris (Miyazaki F) Reviewed
Lugsanangarm Kiattisak, Pianwanit Somsak, Nueangaudom Arthit, Kokpol Sirirat, Tanaka Fumio, Nunthaboot Nadtanet, Ogino Kumiko, Takagi Rikako, Nakanishi Takeshi, Kitamura Masaya, Taniguchi Seiji, Chosrowjan Haik
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY 268 58 - 66 2013.09（ ISSN:1010-6030 ）
Domain order of a bispecific diabody dramatically enhances its antitumor activity beyond structural format conversion: The case of the hEx3 diabody. Reviewed
R. Asano, T. Kumagai, K. Nagai, H. Sanada, T. Nakanishi, Y. Katayose, M. Unno, T. Kudo, M. Umetsu, I. Kumagai et al.
Protein Eng. Des. Sel. 26 ( 5 ) 359 - 367 2013.05
Taniguchi Seiji, Chosrowjan Haik, Tanaka Fumio, Nakanishi Takeshi, Sato Shuta, Haruyama Yoshihiro, Kitamura Masaya
The Chemical Society of Japan BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN 86 ( 3 ) 339 - 350 2013.03（ ISSN:0009-2673 ）
Development of an affinity-matured humanized anti-epidermal growth factor receptor antibody for cancer immunotherapy. Reviewed
T. Nakanishi, T. Maru, K. Tahara, H. Sanada, M. Umetsu, R. Asano, I. Kumagai
Protein Eng. Des. Sel. 26 ( 2 ) 113 - 122 2013.02
A high-affinity gold-binding camel antibody: Antibody engineering for one-pot functionalization of gold nanoparticles as biointerface molecules. Reviewed
T. Hattori, M. Umetsu, T. Nakanishi, S. Sawai, S. Kikuchi, R. Asano, I. Kumagai
Bioconjug. Chem. 23 ( 9 ) 1934 - 1944 2012.09
Construction and humanization of a functional bispecific EGFR CD16 diabody using a refolding system Reviewed
Asano Ryutaro, Nakayama Makoto, Kawaguchi Hiroko, Kubota Tsuguo, Nakanishi Takeshi, Umetsu Mitsuo, Hayashi Hiroki, Katayose Yu, Unno Michiaki, Kudo Toshio, Kumagai Izumi
FEBS JOURNAL 279 ( 2 ) 223 - 233 2012.01（ ISSN:1742-464X ）
In vitro and in vivo antitumor effects of recombinant bispecific antibodies based on humanized anti-EGFR antibody Reviewed
Y. Watanabe, R. Asano, K. Arai, S. Egawa, T. Nakanishi, M. Umetsu, K. Imai, T. Kudo, M. Unno, I. Kumagai et al.
Oncol. Rep. 26 ( 4 ) 949 - 955 2011.10
Biomimetic engineering of modular bispecific antibodies for biomolecule immobilization Reviewed
H. Watanabe, K. Kanazaki, T. Nakanishi, H. Shiotsuka, S. Hatakeyama, M. Kaieda, T. Imamura, M. Umetsu, I. Kumagai
Langmuir 27 ( 16 ) 9656 - 9561 2011.08
Mechanism of HSV infection through soluble adapter-mediated virus bridging to the EGF receptor Reviewed
K. Nakano, M. Kobayashi, K. Nakamura, T. Nakanishi, R. Asano, I. Kumagai, M. Kuwano, J.B. Cohen, J.C. Glorioso et al.
Virology 413 ( 1 ) 12 - 18 2011.04
Cytotoxic Enhancement of a Bispecific Diabody by Format Conversion to Tandem Single-chain Variable Fragment (taFv) THE CASE OF THE hEx3 DIABODY Reviewed
Asano Ryutaro, Ikoma Keiko, Shimomura Ippei, Taki Shintaro, Nakanishi Takeshi, Umetsu Mitsuo, Kumagai Izumi
JOURNAL OF BIOLOGICAL CHEMISTRY 286 ( 3 ) 1812 - 1818 2011.01（ ISSN:0021-9258 ）
Effects of the Disappearance of One Charge on Ultrafast Fluorescence Dynamics of the FMN Binding Protein Reviewed
Chosrowjan Haik, Taniguchi Seiji, Mataga Noboru, Nakanishi Takeshi, Haruyama Yoshihiro, Sato Shuta, Kitamura Masaya, Tanaka Fumio
JOURNAL OF PHYSICAL CHEMISTRY B 114 ( 18 ) 6175 - 6182 2010.05（ ISSN:1520-6106 ）
High Affinity Anti-inorganic Material Antibody Generation by Integrating Graft and Evolution Technologies POTENTIAL OF ANTIBODIES AS BIOINTERFACE MOLECULES Reviewed
Hattori Takamitsu, Umetsu Mitsuo, Nakanishi Takeshi, Togashi Takanari, Yokoo Nozomi, Abe Hiroya, Ohara Satoshi, Adschiri Tadafumi, Kumagai Izumi
JOURNAL OF BIOLOGICAL CHEMISTRY 285 ( 10 ) 7784 - 7793 2010.03（ ISSN:0021-9258 ）
Highly enhanced cytotoxicity of a dimeric bispecific diabody, the hEx3 tetrabody Reviewed
J. Biol. Chem. 285 ( 27 ) 20844 - 20849 2010
Application of the Fc fusion format to generate tag-free bispecific diabodies Reviewed
FEBS J. 277 ( 2 ) 477 - 487 2010
Direct and selective immobilization of proteins by means of an inorganic material-binding peptide: Discussion on functionalization in the elongation to material-binding peptide Reviewed
J. Phys. Chem. B 114 ( 1 ) 480 - 486 2010
Contribution of asparagine residues to the stabilization of a proteinaceous antigen-antibody complex, HyHEL-10-hen egg white lysozyme Reviewed
J. Biol. Chem. 285 ( 10 ) 7686 - 7696 2010
Target cell-restricted apoptosis induction by 528scFv-TRAIL fusion protein specific for human EGFR and expressed in Escherichia coli Reviewed
Int. J. Oncol. 36 ( 5 ) 1229 - 1234 2010
Integration of PEGylation and refolding for renaturation of recombinant proteins from insoluble aggregates produced in bacteria-Application to a single-chain Fv fragment Reviewed
J. Biosci. Bioeng. 109 ( 5 ) 447 - 452 2010
Biotinylated bionanocapsules for displaying diverse ligands toward cell specific delivery Reviewed
J. Biochem. 146 ( 6 ) 867 - 874 2009
Cloning and expression of the MutM gene from obligate anaerobic bacterium Desulfovibrio vulgaris (Miyazaki F) Reviewed
J. Biochem. 145 ( 4 ) 525 - 532 2009
Human Anti-gold Antibodies BIOFUNCTIONALIZATION OF GOLD NANOPARTICLES AND SURFACES WITH ANTI-GOLD ANTIBODIES Reviewed
Watanabe Hideki, Nakanishi Takeshi, Umetsu Mitsuo, Kumagai Izumi
JOURNAL OF BIOLOGICAL CHEMISTRY 283 ( 51 ) 36031 - 36038 2008.12（ ISSN:0021-9258 ）
Diabody-based recombinant formats of humanized IgG-like bispecific antibody with effective retargeting, of lymphocytes to tumor cells Reviewed
Asano Ryutaro, Kawaguchi Hiroko, Watanabe Yasuhiro, Nakanishi Takeshi, Umetsu Mitsuo, Hayashi Hiroki, Katayose Yu, Unno Michiaki, Kudo Toshio, Kumagai Izumi
JOURNAL OF IMMUNOTHERAPY 31 ( 8 ) 752 - 761 2008.10（ ISSN:1524-9557 ）
Thermodynamic consequences of mutations in Vernier zone residues of a humanized anti-human epidermal growth factor receptor murine antibody-528 Reviewed
Makabe Koki, Nakanishi Takeshi, Tsumoto Kouhei, Tanaka Yoshikazu, Kondo Hidemasa, Umetsu Mitsuo, Sone Yukiko, Asano Ryutaro, Kumagai Izumi
JOURNAL OF BIOLOGICAL CHEMISTRY 283 ( 2 ) 1156 - 1166 2008.01（ ISSN:0021-9258 ）
Nanoparticles with affinity for biopolymer: Bioassisted room-temperature selective multistacking of inorganic particles on biopolymer film Reviewed
J. Mater. Res. 23 ( 12 ) 3241 - 3246 2008
Grafting of material-binding function into antibodies: Functionalization by peptide grafting Reviewed
Biochem. Biophys. Res. Commun. 365 ( 4 ) 751 - 757 2008
Critical contribution of VH-VL interaction to reshaping of an antibody: the case of humanization of anti-lysozyme antibody, HyHEL-10 Reviewed
Protein Sci. 17 ( 2 ) 261 - 270 2008
Preferential heterodimerization of a bispecific diabody based on a humanized anti-EGFR antibody 528 Reviewed
Protein Eng. Des. Sel. 21 ( 10 ) 597 - 603 2008
Diabody-based recombinant formats of humanized IgG-like bispecific antibody with effective retargeting of lymphocytes to tumor cells Reviewed
J. Immunother. 31 ( 8 ) 752 - 761 2008
Structural consequences of mutations in interfacial Tyr residues of a protein antigen-anti body complex - The case of HyHEL-10-HEL Reviewed
Shiroishi Mitsunori, Tsumoto Kouhei, Tanaka Yoshikazu, Yokota Akiko, Nakanishi Takeshi, Kondo Hidemasa, Kumagai Izumi
JOURNAL OF BIOLOGICAL CHEMISTRY 282 ( 9 ) 6783 - 6791 2007.03（ ISSN:0021-9258 ）
Highly effective recombinant format of a humanized IgG-like bispecific antibody for cancer immunotherapy with retargeting of lymphocytes to tumor cells Reviewed
J. Biol. Chem. 282 ( 38 ) 27659 - 27665 2007
Detection of structural changes in a cofactor binding protein by using a wheat germ cell-free protein synthesis system coupled with unnatural amino acid probing Reviewed
Proteins 67 ( 3 ) 643 - 652 2007
Structural evidence for guanidine-protein side chain interactions: crystal structure of CutA from Pyrococcus horikoshii in 3 M guanidine hydrochloride Reviewed
Biochem.Biophys. Res. Commun. 323 ( 1 ) 185 - 191 2004
Structural implications for heavy metal-induced reversible assembly and aggregation of a protein: the case of Pyrococcus horikoshii CutA Reviewed
FEBS Lett. 556 ( 42738 ) 167 - 174 2004
Cloning and expression of the superoxide dismutase gene from the obligate anaerobic bacterium Desulfovibrio vulgaris (Miyazaki F) Reviewed
J. Biochem. 133 ( 3 ) 387 - 393 2003
Cloning and expression of the catalase gene from the anaerobic bacterium Desulfovibrio vulgaris (Miyazaki F) Reviewed
J. Biochem. 129 ( 3 ) 357 - 364 2001
次世代医薬開発に向けた抗体工学の最前線 ファージ提示系による親和性の向上
中西 猛,真田英明,熊谷 泉（ Role： Joint author）
シーエムシー出版 2012
抗体医薬の最前線 抗体ドメインの積み木細工による高機能化と医用への展開
熊谷 泉,浅野竜太郎,中西 猛（ Role： Joint author）
シーエムシー出版 2007
Protein-protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information
FEBS J. 277 ( 9 ) 2006 - 2014 2010
Recent aspects of antibodylibrary technologies and their future perspectives
26 ( 3 ) 254 - 257 2007
Fc領域ヘテロ会合化を必要としない非対称二重特異性抗体の作製技術開発
Grant-in-Aid for Scientific Research(C) 2022.04
革新的なバイオ医薬創製に向けた分子連結技術の高度化
Grant-in-Aid for Scientific Research(C) 2017.04
Development of a molecular connection method for generating highly functional antibodies
Grant-in-Aid for Scientific Research(C) 2013.04
Development of a highly efficient method for generating functional antibodies based on grafting natural ligands
Grant-in-Aid for Young Scientists(B) 2011
ボトムアップ的ライブラリー構築による抗体様RNA分解酵素の創製
Grant-in-Aid for Young Scientists(B) 2009
情報バイオ演習
2018 Undergraduate
化学バイオ工学演習A
2018 Undergraduate
生化学Ⅱ
2018 Undergraduate
蛋白質工学特論
2018 Graduate school