Chemically Crosslinked Bispecific Antibodies for Cancer Therapy: Breaking from the Structural Restrictions of the Genetic Fusion Approach 査読

Ueda Asami, Umetsu Mitsuo, Nakanishi Takeshi, Hashikami Kentaro, Nakazawa Hikaru, Hattori Shuhei, Asano Ryutaro, Kumagai Izumi

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   21 ( 3 )   2020年02月

Functional domain order of an anti-EGFR × anti-CD16 bispecific diabody involving NK cell activation 査読

Atsushi Kuwahara, Keisuke Nagai, Takeshi Nakanishi, Izumi Kumagai, and Ryutaro Asano

Int. J. Mol. Sci.   21   8914   2020年

Generation of the rat monoclonal antibody against the extracellular domain of human CD63 by DNA immunization 査読

Liu Xu, Kan-ichiro Ihara, Saori Yoshimura, Daijiro Konno, Akira Tachibana, Takeshi Nakanishi, and Taro Tachibana

Monoclon. Antib. Immunodiagn. Immunother.   39   74 - 76   2020年

Affinity maturation of humanized anti-epidermal growth factor receptor antibody using a modified phage-based open sandwich selection method. 査読

Sanada Hideaki, Kobayashi Kazuki, Oyama Kenji, Maru Takamitsu, Nakanishi Takeshi, Umetsu Mitsuo, Asano Ryutaro, Kumagai Izumi

Scientific Reports   8   5414   2018年04月（ ISSN:2045-2322 ）

Soluble expression in Escherichia coli of a single-domain antibody–tumor necrosis factor α fusion protein specific for epidermal growth factor receptor. 査読

Tomohiro Osaki, Takeshi Nakanishi, Motoshi Aoki, Takahiro Omizu, Daisuke Nishiura, Masaya Kitamura

Monoclon. Antib. Immunodiagn. Immunother.   37 ( 1 )   20 - 25   2018年02月

Photoinduced electron transfer from aromatic amino acids to the excited isoalloxazine in single mutated flavin mononucleotide binding proteins: Effect of the dimer formation on the rate and the electrostatic energy inside the proteins. 査読 国際共著

Nadtanet Nunthaboot, Kiattisak Lugsanangarm, Arthit Nueangaudom, Somsak Pianwanit, Sirirat Kokpol, Fumio Tanaka, Seiji Taniguchi, Haik Chosrowjan, Takeshi Nakanishi, Masaya Kitamura

Comput. Theor. Chem.   1108   1 - 9   2017年

Conformational difference between two subunits in flavin mononucleotide binding protein dimers from Desulfovibrio vulgaris (MF): Molecular dynamics simulation. 査読

N. Nunthaboot, K. Lugsanangarm, S. Pianwanit, S. Kokpol, F. Tanaka, T. Nakanishi, M. Kitamura

Comput. Biol. Chem.   64   113 - 125   2016年10月

Photoinduced electron transfer from aromatic amino acids to the excited isoalloxazine in flavin mononucleotide binding protein. Is the rate in the inverted region of donor – acceptor distance not real? 査読

N. Nunthaboot, K. Lugsanangarm, A. Pianwanit, F. Tanaka, S. Taniguchi, H. Chosrowjan, T. Nakanishi, M. Kitamura et al.

J. Photochem. Photobiol. A: Chem.   326   60 - 68   2016年07月

Generation and characterization of rat monoclonal antibodies against epidermal growth factor receptor. 査読

T. Osaki, C.X. Wang, T. Tachibana, M. Azuma, M. Kitamura, T. Nakanishi

Monoclon. Antib. Immunodiagn. Immunother.   34 ( 6 )   418 - 422   2015年12月

Development of a bispecific antibody tetramerized through hetero-associating peptides. 査読

T. Osaki, S. Fujisawa, M. Kitaguchi, M. Kitamura, T. Nakanishi

FEBS J.   282 ( 22 )   4389 - 4401   2015年11月

Bell-shaped dependence of the rate of ultrafast photoinduced electron transfer from aromatic amino acids to the excited flavin on the donor-acceptor distance in FMN binding proteins 査読

Nunthaboot Nadtanet, Lugsanangarm Kiattisak, Pianwanit Somsak, Kokpol Sirirat, Tanaka Fumio, Taniguchi Seiji, Chosrowjan Haik, Nakanishi Takeshi, Kitamura Masaya

COMPUTATIONAL AND THEORETICAL CHEMISTRY   1030   9 - 16   2014年02月（ ISSN:2210-271X ）

Mechanism of photoinduced electron transfer from tyrosine to the excited flavin in the flavodoxin from Helicobacter pylori. A comparative study with the flavodoxin and flavin mononucleotide binding protein from Desulfovibrio vulgaris (Miyazaki F) 査読

Lugsanangarm Kiattisak, Pianwanit Somsak, Nueangaudom Arthit, Kokpol Sirirat, Tanaka Fumio, Nunthaboot Nadtanet, Ogino Kumiko, Takagi Rikako, Nakanishi Takeshi, Kitamura Masaya, Taniguchi Seiji, Chosrowjan Haik

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY   268   58 - 66   2013年09月（ ISSN:1010-6030 ）

Domain order of a bispecific diabody dramatically enhances its antitumor activity beyond structural format conversion: The case of the hEx3 diabody. 査読

R. Asano, T. Kumagai, K. Nagai, H. Sanada, T. Nakanishi, Y. Katayose, M. Unno, T. Kudo, M. Umetsu, I. Kumagai et al.

Protein Eng. Des. Sel.   26 ( 5 )   359 - 367   2013年05月

A Key Factor for Ultrafast Rates of Photoinduced Electron Transfer among Five Flavin Mononucleotide Binding Proteins: Effect of Negative, Positive, and Neutral Charges at Residue 13 on the Rate 査読

Taniguchi Seiji, Chosrowjan Haik, Tanaka Fumio, Nakanishi Takeshi, Sato Shuta, Haruyama Yoshihiro, Kitamura Masaya

The Chemical Society of Japan　BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN   86 ( 3 )   339 - 350   2013年03月（ ISSN:0009-2673 ）

Development of an affinity-matured humanized anti-epidermal growth factor receptor antibody for cancer immunotherapy. 査読

T. Nakanishi, T. Maru, K. Tahara, H. Sanada, M. Umetsu, R. Asano, I. Kumagai

Protein Eng. Des. Sel.   26 ( 2 )   113 - 122   2013年02月

A high-affinity gold-binding camel antibody: Antibody engineering for one-pot functionalization of gold nanoparticles as biointerface molecules. 査読

T. Hattori, M. Umetsu, T. Nakanishi, S. Sawai, S. Kikuchi, R. Asano, I. Kumagai

Bioconjug. Chem.   23 ( 9 )   1934 - 1944   2012年09月

Construction and humanization of a functional bispecific EGFR CD16 diabody using a refolding system 査読

Asano Ryutaro, Nakayama Makoto, Kawaguchi Hiroko, Kubota Tsuguo, Nakanishi Takeshi, Umetsu Mitsuo, Hayashi Hiroki, Katayose Yu, Unno Michiaki, Kudo Toshio, Kumagai Izumi

FEBS JOURNAL   279 ( 2 )   223 - 233   2012年01月（ ISSN:1742-464X ）

In vitro and in vivo antitumor effects of recombinant bispecific antibodies based on humanized anti-EGFR antibody 査読

Y. Watanabe, R. Asano, K. Arai, S. Egawa, T. Nakanishi, M. Umetsu, K. Imai, T. Kudo, M. Unno, I. Kumagai et al.

Oncol. Rep.   26 ( 4 )   949 - 955   2011年10月

Biomimetic engineering of modular bispecific antibodies for biomolecule immobilization 査読

H. Watanabe, K. Kanazaki, T. Nakanishi, H. Shiotsuka, S. Hatakeyama, M. Kaieda, T. Imamura, M. Umetsu, I. Kumagai

Langmuir   27 ( 16 )   9656 - 9561   2011年08月

Mechanism of HSV infection through soluble adapter-mediated virus bridging to the EGF receptor 査読

K. Nakano, M. Kobayashi, K. Nakamura, T. Nakanishi, R. Asano, I. Kumagai, M. Kuwano, J.B. Cohen, J.C. Glorioso et al.

Virology   413 ( 1 )   12 - 18   2011年04月

Cytotoxic Enhancement of a Bispecific Diabody by Format Conversion to Tandem Single-chain Variable Fragment (taFv) THE CASE OF THE hEx3 DIABODY 査読

Asano Ryutaro, Ikoma Keiko, Shimomura Ippei, Taki Shintaro, Nakanishi Takeshi, Umetsu Mitsuo, Kumagai Izumi

JOURNAL OF BIOLOGICAL CHEMISTRY   286 ( 3 )   1812 - 1818   2011年01月（ ISSN:0021-9258 ）

Effects of the Disappearance of One Charge on Ultrafast Fluorescence Dynamics of the FMN Binding Protein 査読

Chosrowjan Haik, Taniguchi Seiji, Mataga Noboru, Nakanishi Takeshi, Haruyama Yoshihiro, Sato Shuta, Kitamura Masaya, Tanaka Fumio

JOURNAL OF PHYSICAL CHEMISTRY B   114 ( 18 )   6175 - 6182   2010年05月（ ISSN:1520-6106 ）

High Affinity Anti-inorganic Material Antibody Generation by Integrating Graft and Evolution Technologies POTENTIAL OF ANTIBODIES AS BIOINTERFACE MOLECULES 査読

Hattori Takamitsu, Umetsu Mitsuo, Nakanishi Takeshi, Togashi Takanari, Yokoo Nozomi, Abe Hiroya, Ohara Satoshi, Adschiri Tadafumi, Kumagai Izumi

JOURNAL OF BIOLOGICAL CHEMISTRY   285 ( 10 )   7784 - 7793   2010年03月（ ISSN:0021-9258 ）

Highly enhanced cytotoxicity of a dimeric bispecific diabody, the hEx3 tetrabody 査読

R. Asano, K. Ikoma, Y. Sone, H. Kawaguchi, S.Taki, H. Hayashi, T. Nakanishi, M. Umetsu et al.

J. Biol. Chem.   285 ( 27 )   20844 - 20849   2010年

Application of the Fc fusion format to generate tag-free bispecific diabodies 査読

R. Asano, K. Ikoma, H. Kawaguchi, Y. Ishiyama, T. Nakanishi, M. Umetsu, H. Hayashi et al.

FEBS J.   277 ( 2 )   477 - 487   2010年

Direct and selective immobilization of proteins by means of an inorganic material-binding peptide: Discussion on functionalization in the elongation to material-binding peptide 査読

N. Yokoo, T. Togashi, M. Umetsu, K. Tsumoto, T. Hattori, T. Nakanishi, S. Ohara, S. Takami et al.

J. Phys. Chem. B   114 ( 1 )   480 - 486   2010年

Contribution of asparagine residues to the stabilization of a proteinaceous antigen-antibody complex, HyHEL-10-hen egg white lysozyme 査読

A. Yokota, K. Tsumoto, M. Shiroishi, T. Nakanishi, H. Kondo, I. Kumagai

J. Biol. Chem.   285 ( 10 )   7686 - 7696   2010年

Target cell-restricted apoptosis induction by 528scFv-TRAIL fusion protein specific for human EGFR and expressed in Escherichia coli 査読

A. Badran, R. Asano, M. Nakayama, Y. Watanabe, T. Nakanishi, M. Umetsu, H. Hayashi et al.

Int. J. Oncol.   36 ( 5 )   1229 - 1234   2010年

Integration of PEGylation and refolding for renaturation of recombinant proteins from insoluble aggregates produced in bacteria-Application to a single-chain Fv fragment 査読

I. Kumagai, R. Asano, T. Nakanishi, K. Hashikami, S. Tanaka, A. Badran, H. Sanada, M. Umetsu

J. Biosci. Bioeng.   109 ( 5 )   447 - 452   2010年

Cloning and expression of the MutM gene from obligate anaerobic bacterium Desulfovibrio vulgaris (Miyazaki F) 査読

H. Sanada, T. Nakanishi, H. Inoue,M. Kitamura

J. Biochem.   145 ( 4 )   525 - 532   2009年

Biotinylated bionanocapsules for displaying diverse ligands toward cell specific delivery 査読

T. Shishido, Y. Azumi, T. Nakanishi, M. Umetsu, T. Tanaka, C. Ogino, H. Fukuda, A. Kondo

J. Biochem.   146 ( 6 )   867 - 874   2009年

Human Anti-gold Antibodies BIOFUNCTIONALIZATION OF GOLD NANOPARTICLES AND SURFACES WITH ANTI-GOLD ANTIBODIES 査読

Watanabe Hideki, Nakanishi Takeshi, Umetsu Mitsuo, Kumagai Izumi

JOURNAL OF BIOLOGICAL CHEMISTRY   283 ( 51 )   36031 - 36038   2008年12月（ ISSN:0021-9258 ）

Diabody-based recombinant formats of humanized IgG-like bispecific antibody with effective retargeting, of lymphocytes to tumor cells 査読

Asano Ryutaro, Kawaguchi Hiroko, Watanabe Yasuhiro, Nakanishi Takeshi, Umetsu Mitsuo, Hayashi Hiroki, Katayose Yu, Unno Michiaki, Kudo Toshio, Kumagai Izumi

JOURNAL OF IMMUNOTHERAPY   31 ( 8 )   752 - 761   2008年10月（ ISSN:1524-9557 ）

Thermodynamic consequences of mutations in Vernier zone residues of a humanized anti-human epidermal growth factor receptor murine antibody-528 査読

Makabe Koki, Nakanishi Takeshi, Tsumoto Kouhei, Tanaka Yoshikazu, Kondo Hidemasa, Umetsu Mitsuo, Sone Yukiko, Asano Ryutaro, Kumagai Izumi

JOURNAL OF BIOLOGICAL CHEMISTRY   283 ( 2 )   1156 - 1166   2008年01月（ ISSN:0021-9258 ）

Grafting of material-binding function into antibodies: Functionalization by peptide grafting 査読

T. Hattori, M. Umetsu, T. Nakanishi, K. Tsumoto, S. Ohara, H. Abe, M. Naito, R. Asano et al.

Biochem. Biophys. Res. Commun.   365 ( 4 )   751 - 757   2008年

Critical contribution of VH-VL interaction to reshaping of an antibody: the case of humanization of anti-lysozyme antibody, HyHEL-10 査読

T. Nakanishi, K. Tsumoto, A. Yokota, H. Kondo, I. Kumagai

Protein Sci.   17 ( 2 )   261 - 270   2008年

Preferential heterodimerization of a bispecific diabody based on a humanized anti-EGFR antibody 528 査読

R.Asano, Y. Sone, K. Ikoma, H. Hayashi, T. Nakanishi, M. Umetsu, Y. Katayose, M. Unno et al.

Protein Eng. Des. Sel.   21 ( 10 )   597 - 603   2008年

Diabody-based recombinant formats of humanized IgG-like bispecific antibody with effective retargeting of lymphocytes to tumor cells 査読

R. Asano, H. Kawaguchi, Y. Watanabe, T. Nakanishi, M. Umetsu, H. Hayashi, Y. Katayose et al.

J. Immunother.   31 ( 8 )   752 - 761   2008年

Nanoparticles with affinity for biopolymer: Bioassisted room-temperature selective multistacking of inorganic particles on biopolymer film 査読

M. Umetsu, T. Hattori, S. Kikuchi, I. Muto, T. Nakanishi, H. Watanabe, I. Kumagai

J. Mater. Res.   23 ( 12 )   3241 - 3246   2008年

Structural consequences of mutations in interfacial Tyr residues of a protein antigen-anti body complex - The case of HyHEL-10-HEL 査読

Shiroishi Mitsunori, Tsumoto Kouhei, Tanaka Yoshikazu, Yokota Akiko, Nakanishi Takeshi, Kondo Hidemasa, Kumagai Izumi

JOURNAL OF BIOLOGICAL CHEMISTRY   282 ( 9 )   6783 - 6791   2007年03月（ ISSN:0021-9258 ）

Highly effective recombinant format of a humanized IgG-like bispecific antibody for cancer immunotherapy with retargeting of lymphocytes to tumor cells 査読

R. Asano, Y. Watanabe, H. Kawaguchi, H. Fukazawa, T. Nakanishi, M. Umetsu, H. Hayashi et al.

J. Biol. Chem.   282 ( 38 )   27659 - 27665   2007年

Detection of structural changes in a cofactor binding protein by using a wheat germ cell-free protein synthesis system coupled with unnatural amino acid probing 査読

M. Abe, S. Ohno, T. Yokogawa, T. Nakanishi, F. Arisaka, T. Hosoya, T. Hiramatsu, M. Suzuki et al.

Proteins   67 ( 3 )   643 - 652   2007年

Structural evidence for guanidine-protein side chain interactions: crystal structure of CutA from Pyrococcus horikoshii in 3 M guanidine hydrochloride 査読

Y. Tanaka, K. Tsumoto, M. Umetsu, T. Nakanishi, Y. Yasutake, N. Sakai, M. Yao, I. Tanaka et al.

Biochem.Biophys. Res. Commun.   323 ( 1 )   185 - 191   2004年

Structural implications for heavy metal-induced reversible assembly and aggregation of a protein: the case of Pyrococcus horikoshii CutA 査読

Y. Tanaka, K. Tsumoto, T. Nakanishi, Y. Yasutake, N. Sakai, M. Yao, I. Tanaka, I. Kumagai

FEBS Lett.   556 ( 42738 )   167 - 174   2004年

Cloning and expression of the superoxide dismutase gene from the obligate anaerobic bacterium Desulfovibrio vulgaris (Miyazaki F) 査読

T. Nakanishi, H. Inoue, M. Kitamura

J. Biochem.   133 ( 3 )   387 - 393   2003年

Cloning and expression of the catalase gene from the anaerobic bacterium Desulfovibrio vulgaris (Miyazaki F) 査読

M. Kitamura, T. Nakanishi, S. Kojima, I. Kumagai, H. Inoue

J. Biochem.   129 ( 3 )   357 - 364   2001年

ボトムアップ的ライブラリー構築による抗体様RNA分解酵素の創製

若手研究(B)  2009年

物質化学生命系特別研究第1

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