Updated on 2023/04/10

写真a

 
TACHIBANA Taro
 
Organization
Graduate School of Engineering Division of Science and Engineering for Materials, Chemistry and Biology Professor
School of Engineering Department of Chemistry and Bioengineering
Title
Professor
Affiliation
Institute of Engineering
Affiliation campus
Sugimoto Campus

Position

  • Graduate School of Engineering Division of Science and Engineering for Materials, Chemistry and Biology 

    Professor  2022.04 - Now

  • School of Engineering Department of Chemistry and Bioengineering 

    Professor  2022.04 - Now

Degree

  • Doctor of Medicine ( Osaka University )

Research Areas

  • Life Science / Cell biology  / 分子細胞生物学

Research Interests

  • 抗体医薬品

  • モノクローナル抗体

Research subject summary

  • 現在、モノクローナル抗体はバイオ分野のツールとしてだけでなく、検査薬や治療薬(抗体医薬品)として活用され、私たちの暮らしに欠かせないものとなっています。抗体医薬品は癌やリウマチなどの疾患の治療に目覚ましい成果を上げ、今や世界の医薬品売上ランキングの上位を占めるまでに至っています。今後さらに抗体医薬品への期待は高まるばかりですが、高特異性・高親和性の抗体作製は非常に難易度が高く、新しい高性能抗体作製技術の開発が望まれています。優れた技術の確立は抗体医薬品新薬の開発に直結します。つまり、抗体作製技術の開発研究は、創薬研究そのものと言えます。これまでのモノクローナル抗体の作製にはマウスやラットが用いられてきました。創薬生命工学研究室では、マウスやラットだけでなく、ウサギやウシさらにはヒトなど、より優れた免疫システムを持つ動物を利用し、ゲノム編集技術など高度な生命工学手法を駆使し、高性能な抗体を高効率に作製する方法の開発に取り組んでいます。さらに、国内外の研究者と積極的に共同研究を行い、特定の幹細胞や分化細胞特異的抗原など、様々なターゲットに対する抗体作製とそれら抗体のユニークな利用法についての応用研究を行っています。

Research Career

  • Development of novel method for generation of monoclonal antibodies

    monoclonal antibody  Individual

    1993.04 

Professional Memberships

  • Japan Society for Cell Biology

      Domestic

Awards

  • CSF

    2001  

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    Country:Japan

  • 山村賞

    1995  

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    Country:Japan

Job Career (off-campus)

  • 株式会社細胞工学研究所   代表取締役

    2010.02 - Now

  • 米国マサチューセッツ大学医学部   客員研究員

    2006.04 - 2007.03

  • 大阪大学大学院医学研究科・助手   -

    1998 - 2001

Education

  • Osaka University   Doctor's Course   Graduated/Completed

    - 1996

  • Osaka Prefecture University   Applied chemistry     Graduated/Completed

    - 1990

Papers

  • Generation of Rat Monoclonal Antibody for Mouse Nucleolin by Immunization of Ferroptosis-Induced Hepa 1-6 Cells. Reviewed

    Yokoyama C, Kobayashi S, Harada Y, Nishino Y, Fujii J, Tachibana T

    Monoclonal antibodies in immunodiagnosis and immunotherapy   41 ( 5 )   255 - 259   2022.10

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/mab.2022.0005

    PubMed

  • SARS-CoV-2 ORF6 disrupts nucleocytoplasmic trafficking to advance viral replication. Reviewed

    Miyamoto Y, Itoh Y, Suzuki T, Tanaka T, Sakai Y, Koido M, Hata C, Wang CX, Otani M, Moriishi K, Tachibana T, Kamatani Y, Yoneda Y, Okamoto T, Oka M

    Communications biology   5 ( 1 )   483   2022.05

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s42003-022-03427-4

    PubMed

  • Establishment of monoclonal antibodies broadly neutralize infection of hepatitis B virus. Reviewed

    Zhang H, Itoh Y, Suzuki T, Ihara KI, Tanaka T, Haga S, Enatsu H, Yumiya M, Kimura M, Takada A, Itoh D, Shibazaki Y, Nakao S, Yoshio S, Miyakawa K, Miyamoto Y, Sasaki H, Kajita T, Sugiyama M, Mizokami M, Tachibana T, Ryo A, Moriishi K, Miyoshi E, Kanto T, Okamoto T, Matsuura Y

    Microbiology and immunology   66 ( 4 )   179 - 192   2022.04( ISSN:0385-5600

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/1348-0421.12964

    PubMed

  • Unusual nucleosome formation and transcriptome influence by the histone H3mm18 variant. Reviewed

    Hirai S, Tomimatsu K, Miyawaki-Kuwakado A, Takizawa Y, Komatsu T, Tachibana T, Fukushima Y, Takeda Y, Negishi L, Kujirai T, Koyama M, Ohkawa Y, Kurumizaka H

    Nucleic acids research   50 ( 1 )   72 - 91   2022.01( ISSN:0305-1048

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/nar/gkab1137

    PubMed

  • Septin-microtubule association via a motif unique to isoform 1 of septin 9 tunes stress fibers. Reviewed

    Kuzmić M, Castro Linares G, Leischner Fialová J, Iv F, Salaün D, Llewellyn A, Gomes M, Belhabib M, Liu Y, Asano K, Rodrigues M, Isnardon D, Tachibana T, Koenderink GH, Badache A, Mavrakis M, Verdier-Pinard P

    Journal of cell science   135 ( 1 )   2022.01( ISSN:0021-9533

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1242/jcs.258850

    PubMed

  • Gαq modulates the energy metabolism of osteoclasts. Reviewed

    Sushmita Chakraborty, Bianca Handrick, Dayoung Yu, Konrad A Bode, Anna Hafner, Judith Schenz, Dominik Schaack, Florian Uhle, Taro Tachibana, Shigeki Kamitani, Thomas Vogl, Katharina F Kubatzky

    Frontiers in cellular and infection microbiology   12   1016299 - 1016299   2022

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    INTRODUCTION: The bacterial protein toxin Pasteurella multocida toxin (PMT) mediates RANKL-independent osteoclast differentiation. Although these osteoclasts are smaller, their resorptive activity is high which helps in efficient destruction of nasal turbinate bones of pigs. METHODS: The proteome of bone marrow-derived macrophages differentiated into osteoclasts with either RANKL or PMT was analysed. The results were verified by characterizing the metabolic activity using Seahorse analysis, a protein translation assay, immunoblots, real-time PCR as well as flow cytometry-based monitoring of mitochondrial activity and ROS production. A Gαq overexpression system using ER-Hoxb8 cells was used to identify Gαq-mediated metabolic effects on osteoclast differentiation and function. RESULTS: PMT induces the upregulation of metabolic pathways, which included strong glycolytic activity, increased expression of GLUT1 and upregulation of the mTOR pathway. As OxPhos components were expressed more efficiently, cells also displayed increased mitochondrial respiration. The heterotrimeric G protein Gαq plays a central role in this hypermetabolic cell activation as it triggers mitochondrial relocalisation of pSerSTAT3 and an increase in OPA1 expression. This seems to be caused by a direct interaction between STAT3 and OPA1 resulting in enhanced mitochondrial respiration. Overexpression of Gαq mimicked the hypermetabolic phenotype observed for PMT-induced osteoclasts and resulted in higher glycolytic and mitochondrial activity as well as increased bone resorptive activity. In addition, rheumatoid arthritis (RA) patients showed an increase in GNAQ expression, especially in the synovial fluid. DISCUSSION: Our study suggests that Gαq plays a key role in PMT-induced osteoclastogenesis. Enhanced expression of GNAQ at the site of inflammation in RA patients indicates its pathophysiological relevance in the context of inflammatory bone disorders.

    DOI: 10.3389/fcimb.2022.1016299

    PubMed

  • Canopy Homolog 2 as a Novel Molecular Target in Hepatocarcinogenesis. Reviewed

    Kakehashi A, Suzuki S, Shiota M, Raymo N, Gi M, Tachibana T, Stefanov V, Wanibuchi H

    Cancers   13 ( 14 )   2021.07( ISSN:2072-6694

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.3390/cancers13143613

    PubMed

  • Wound, pressure ulcer and burn guidelines – 6: Guidelines for the management of burns, second edition Reviewed

    Yoshino Y.

    Journal of Dermatology   47 ( 11 )   1207 - 1235   2020.11( ISSN:03852407

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/1346-8138.15335

  • Detection of Rhizopus-specific antigen in human and murine serum and bronchoalveolar lavage. Reviewed

    Shibata W, Niki M, Sato K, Fujimoto H, Yamada K, Watanabe T, Miyazaki Y, Asai K, Obata Y, Tachibana T, Kawaguchi T, Kaneko Y, Kakeya H

    Medical mycology   58 ( 7 )   958 - 964   2020.10( ISSN:1369-3786

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/mmy/myaa001

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  • Wound, pressure ulcer and burn guidelines – 4: Guidelines for the management of connective tissue disease/vasculitis-associated skin ulcers Reviewed

    Fujimoto M.

    Journal of Dermatology   47 ( 10 )   1071 - 1109   2020.10( ISSN:03852407

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/1346-8138.15186

  • Outcome of Allogeneic Hematopoietic Stem Cell Transplantation in Patients with Myelodysplastic/Myeloproliferative Neoplasms-Unclassifiable: A Retrospective Nationwide Study of the Japan Society for Hematopoietic Cell Transplantation Reviewed

    Kurosawa S.

    Biology of Blood and Marrow Transplantation   26 ( 9 )   1607 - 1611   2020.09( ISSN:10838791

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bbmt.2020.05.013

  • Wound, pressure ulcer and burn guidelines – 1: Guidelines for wounds in general, second edition Reviewed

    Hasegawa M.

    Journal of Dermatology   47 ( 8 )   807 - 833   2020.08( ISSN:03852407

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/1346-8138.15401

  • Generation of the Rat Monoclonal Antibody Against the Extracellular Domain of Human CD63 by DNA Immunization. Reviewed

    Xu L, Ihara KI, Yoshimura S, Konno D, Tachibana A, Nakanishi T, Tachibana T

    Monoclonal antibodies in immunodiagnosis and immunotherapy   39 ( 3 )   74 - 76   2020.06

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/mab.2020.0007

    PubMed

  • A straightforward approach to antibodies recognising cancer specific glycopeptidic neoepitopes. Reviewed

    Wakui H, Tanaka Y, Ose T, Matsumoto I, Kato K, Min Y, Tachibana T, Sato M, Naruchi K, Martin FG, Hinou H, Nishimura SI

    Chemical science   11 ( 19 )   4999 - 5006   2020.04( ISSN:2041-6520

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1039/d0sc00317d

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  • Preparation and Characterization of a Novel Bispecific Antibody That Targets Her2 and BSH for Boron Neutron Capture Therapy

    立花 太郎, 中西 猛, 真田 悠生

    KURNS Progress Report 2019   -   2020

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    Publishing type:Research paper (scientific journal)  

  • Carbohydrate 3′-sialyllactose as a novel target for theranostics in pancreatic ductal adenocarcinoma Reviewed

    Higashi K.

    Tumor Biology   42 ( 10 )   2020( ISSN:10104283

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1177/1010428320965279

  • PACT/PRKRA and p53 regulate transcriptional activity of DMRT1. Reviewed

    Fujitani K, Otomo A, Nagayama Y, Tachibana T, Kato R, Kawashima Y, Kodera Y, Kato T, Takada S, Tamura K, Takamatsu N, Ito M

    Genetics and molecular biology   43 ( 2 )   e20190017   2020( ISSN:1415-4757

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1590/1678-4685-GMB-2019-0017

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  • Hematopoietic Stem Cell Transplantation From a Related Donor with Human Leukocyte Antigen 1-Antigen Mismatch in the Graft-Versus-Host Direction Using Low-dose Anti-thymocyte Globulin Reviewed

    Kanda J.

    Cell Transplantation   29   2020( ISSN:09636897

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1177/0963689720976567

  • The effect of melphalan dose and total body irradiation as reduced-intensity conditioning for acute lymphoblastic leukemia patients undergoing allogeneic stem cell transplantation Reviewed

    Harada K.

    Leukemia and Lymphoma   60 ( 14 )   3521 - 3528   2019.12( ISSN:10428194

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1080/10428194.2019.1636986

  • Crumbs3 is a critical factor that regulates invasion and metastasis of colon adenocarcinoma via the specific interaction with FGFR1. Reviewed

    Hidekazu Iioka, Ken Saito, Masakiyo Sakaguchi, Taro Tachibana, Keiichi Homma, Eisaku Kondo

    International journal of cancer   145 ( 10 )   2740 - 2753   2019.11( ISSN:0020-7136

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Epithelial cell polarity regulator Crumbs3 (Crb3), a mammalian homolog within the Drosophila Crb gene family, was initially identified as an essential embryonic development factor. It is recently implicated in tumor suppression, though its specific functions are controversial. We here demonstrate that Crb3 strongly promotes tumor invasion and metastasis of human colon adenocarcinoma cells. Crb3 centrality to tumor migration was supported by strong expression at invasive front and metastatic foci of colonic adenocarcinoma of the patient tissues. Accordingly, two different Crb3-knockout (KO) lines, Crb3-KO (Crb3 -/-) DLD-1 and Crb3-KO WiDr from human colonic adenocarcinomas, were generated by the CRISPR-Cas9 system. Crb3-KO DLD-1 cells exhibited loss of cellular mobility in vitro and dramatic suppression of liver metastases in vivo in contrast to the wild type of DLD-1. Unlike DLD-1, Crb3-KO WiDr mobility and metastasis were unaffected, which were similar to wild-type WiDr. Proteome analysis of Crb3-coimmunopreciptated proteins identified different respective fibroblast growth factor receptor (FGFR) isotypes specifically bound to Crb3 isoform a through their intracellular domain. In DLD-1, Crb3 showed membranous localization of FGFR1 leading to its functional activation, whereas Crb3 bound to cytoplasmic FGFR4 in WiDr without FGFR1 expression, leading to cellular growth. Correlative expression between Crb3 and FGFR1 was consistently detected in primary and metastatic colorectal cancer patient tissues. Taking these together, Crb3 critically accelerates cell migration, namely invasion and metastasis of human colon cancers, through specific interaction to FGFR1 on colon cancer cells.

    DOI: 10.1002/ijc.32336

    PubMed

  • Chromatin-bound CRM1 recruits SET-Nup214 and NPM1c onto <i>HOX</i> clusters causing aberrant <i>HOX</i> expression in leukemia cells. Reviewed

    Oka M, Mura S, Otani M, Miyamoto Y, Nogami J, Maehara K, Harada A, Tachibana T, Yoneda Y, Ohkawa Y

    eLife   8   2019.11

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    We previously demonstrated that CRM1, a major nuclear export factor, accumulates at Hox cluster regions to recruit nucleoporin-fusion protein Nup98HoxA9, resulting in robust activation of Hox genes (Oka et al., 2016). However, whether this phenomenon is general to other leukemogenic proteins remains unknown. Here, we show that two other leukemogenic proteins, nucleoporin-fusion SET-Nup214 and the NPM1 mutant, NPM1c, which contains a nuclear export signal (NES) at its C-terminus and is one of the most frequent mutations in acute myeloid leukemia, are recruited to the HOX cluster region via chromatin-bound CRM1, leading to HOX gene activation in human leukemia cells. Furthermore, we demonstrate that this mechanism is highly sensitive to a CRM1 inhibitor in leukemia cell line. Together, these findings indicate that CRM1 acts as a key molecule that connects leukemogenic proteins to aberrant HOX gene regulation either via nucleoporin-CRM1 interaction (for SET-Nup214) or NES-CRM1 interaction (for NPM1c).

    DOI: 10.7554/eLife.46667

    PubMed

  • Different impact of BCR-ABL transcripts on allogeneic hematopoietic cell transplantation from different graft sources for Ph + ALL with minimal residual disease. Reviewed

    Nishiwaki S, Mizuta S, Ohashi K, Fukuda T, Uchida N, Tachibana T, Onizuka M, Ozawa Y, Onishi Y, Takahashi S, Eto T, Nakamae H, Tanaka J, Ichinohe T, Atsuta Y, Kako S, Adult Acute Lymphoblastic Leukemia Working Group of the Japan Society for Hematopoietic Cell Transplantation

    American journal of hematology   94 ( 11 )   E301 - E305   2019.11( ISSN:0361-8609

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/ajh.25612

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  • GPAT2 is required for piRNA biogenesis, transposon silencing, and maintenance of spermatogonia in mice†. Reviewed

    Yusuke Shiromoto, Satomi Kuramochi-Miyagawa, Ippei Nagamori, Shinichiro Chuma, Tatsuhiko Arakawa, Toru Nishimura, Hidetoshi Hasuwa, Taro Tachibana, Masahito Ikawa, Toru Nakano

    Biology of reproduction   101 ( 1 )   248 - 256   2019.07( ISSN:0006-3363

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    PIWI-interacting RNAs (piRNAs), a subclass of germ cell-specific noncoding small RNAs, are essential for de novo DNA methylation of retrotransposon genes in embryonic testes. PIWIL2/MILI, one of three mouse PIWI family members, is indispensable for piRNA production, DNA methylation of retrotransposons presumably via piRNA, and normal spermatogenesis. In vitro analysis using germline stem cells (GS cells) revealed that glycerol-3-phosphate acyltransferase 2 (GPAT2), which is a mitochondrial outer membrane protein involved in generation of lysophosphatidic acid (LPA) and highly expressed in testes, plays important roles in spermatogenesis. Namely, GPAT2 binds to PIWIL2 and is closely involved in the biogenesis of piRNAs; this process is independent of its enzymatic activity on LPA. However, GS cells recapitulate only a limited phase of spermatogenesis and the biological functions of GPAT2 remain largely unknown. In this study, we generated GPAT2-deficient mice and conducted comprehensive analyses. The deficient mice showed defective piRNA production and subsequent de-silencing of IAP and Line-1 retrotransposons in fetal testes. In addition, apoptosis of pachytene spermatocytes was observed. These abnormalities were all common to the phenotype of PIWIL2-deficient mice, in which piRNA production was impaired. GPAT2-deficient mice exhibited apoptosis in spermatogonia at the neonatal stage, which was not observed in PIWIL2-deficient mice. These data show that GPAT2 plays a critical role in preventing apoptosis in spermatogonia.

    DOI: 10.1093/biolre/ioz056

    PubMed

  • GPAT2 is required for piRNA biogenesis, transposon silencing, and maintenance of spermatogonia in mice. Reviewed

    Shiromoto Y, Kuramochi-Miyagawa S, Nagamori I, Chuma S, Arakawa T, Nishimura T, Hasuwa H, Tachibana T, Ikawa M, Nakano T

    Biology of reproduction   101 ( 1 )   248 - 256   2019.04( ISSN:0006-3363

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/biolre/ioz056

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  • Crumbs3 is a critical factor that regulates invasion and metastasis of colon adenocarcinoma via the specific interaction with FGFR1. Reviewed

    Iioka H, Saito K, Sakaguchi M, Tachibana T, Homma K, Kondo E

    International journal of cancer   145 ( 10 )   2740 - 2753   2019.04( ISSN:0020-7136

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/ijc.32336

    PubMed

  • The <i>STK35</i> locus contributes to normal gametogenesis and encodes a lncRNA responsive to oxidative stress. Reviewed

    Miyamoto Y, Whiley PAF, Goh HY, Wong C, Higgins G, Tachibana T, McMenamin PG, Mayne L, Loveland KL

    Biology open   7 ( 8 )   2018.08

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1242/bio.032631

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  • The <i>STK35</i> locus contributes to normal gametogenesis and encodes a lncRNA responsive to oxidative stress. Reviewed

    Miyamoto Y, Whiley PAF, Goh HY, Wong C, Higgins G, Tachibana T, McMenamin PG, Mayne L, Loveland KL

    Biology open   7 ( 8 )   2018.08( ISSN:2046-6390

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1242/bio.032631

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  • EB1-binding-myomegalin protein complex promotes centrosomal microtubules functions Reviewed

    Bouguenina Habib, Salaun Daniele, Mangon Aurelie, Muller Leslie, Baudelet Emilie, Camoin Luc, Tachibana Taro, Cianferani Sarah, Audebert Stephane, Verdier-Pinard Pascal, Badache Ali

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   114 ( 50 )   E10687 - E10696   2017.12( ISSN:0027-8424

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1073/pnas.1705682114

  • EB1-binding-myomegalin protein complex promotes centrosomal microtubules functions Reviewed

    Habib Bouguenina, Daniele Salaun, Aurelie Mangon, Leslie Muller, Emilie Baudelet, Luc Camoin, Taro Tachibana, Sarah Cianferani, Stephane Audebert, Pascal Verdier-Pinard, Ali Badache

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   114 ( 50 )   E10687 - E10696   2017.12( ISSN:0027-8424

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    Publishing type:Research paper (scientific journal)  

    Control of microtubule dynamics underlies several fundamental processes such as cell polarity, cell division, and cell motility. To gain insights into the mechanisms that control microtubule dynamics during cell motility, we investigated the interactome of the microtubule plus-end-binding protein end-binding 1 (EB1). Via molecular mapping and cross-linking mass spectrometry we identified and characterized a large complex associating a specific isoform of myomegalin termed "SMYLE" (for short myomegalin-like EB1 binding protein), the PKA scaffolding protein AKAP9, and the pericentrosomal protein CDK5RAP2. SMYLE was associated through an evolutionarily conserved N-terminal domain with AKAP9, which in turn was anchored at the centrosome via CDK5RAP2. SMYLE connected the pericentrosomal complex to the microtubule-nucleating complex (gamma-TuRC) via Galectin-3-binding protein. SMYLE associated with nascent centrosomal microtubules to promote microtubule assembly and acetylation. Disruption of SMYLE interaction with EB1 or AKAP9 prevented microtubule nucleation and their stabilization at the leading edge of migrating cells. In addition, SMYLE depletion led to defective astral microtubules and abnormal orientation of the mitotic spindle and triggered G1 cell-cycle arrest, which might be due to defective centrosome integrity. As a consequence, SMYLE loss of function had a profound impact on tumor cell motility and proliferation, suggesting that SMYLE might be an important player in tumor progression.

    DOI: 10.1073/pnas.1705682114

    PubMed

  • A statistical image analysis framework for pore-free islands derived from heterogeneity distribution of nuclear pore complexes Reviewed

    Mimura Yasuhiro, Takemoto Satoko, Tachibana Taro, Ogawa Yutaka, Nishimura Masaomi, Yokota Hideo, Imamoto Naoko

    SCIENTIFIC REPORTS   7   2017.11( ISSN:2045-2322

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41598-017-16386-2

  • A statistical image analysis framework for pore-free islands derived from heterogeneity distribution of nuclear pore complexes Reviewed

    Yasuhiro Mimura, Satoko Takemoto, Taro Tachibana, Yutaka Ogawa, Masaomi Nishimura, Hideo Yokota, Naoko Imamoto

    SCIENTIFIC REPORTS   7 ( 1 )   16315   2017.11( ISSN:2045-2322

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    Publishing type:Research paper (scientific journal)  

    Nuclear pore complexes (NPCs) maintain cellular homeostasis by mediating nucleocytoplasmic transport. Although cyclin-dependent kinases (CDKs) regulate NPC assembly in interphase, the location of NPC assembly on the nuclear envelope is not clear. CDKs also regulate the disappearance of pore-free islands, which are nuclear envelope subdomains; this subdomain gradually disappears with increase in homogeneity of the NPC in response to CDK activity. However, a causal relationship between pore-free islands and NPC assembly remains unclear. Here, we elucidated mechanisms underlying NPC assembly from a new perspective by focusing on pore-free islands. We proposed a novel framework for image-based analysis to automatically determine the detailed 'landscape'of pore-free islands from a large quantity of images, leading to the identification of NPC intermediates that appear in pore-free islands with increased frequency in response to CDK activity. Comparison of the spatial distribution between simulated and the observed NPC intermediates within pore-free islands showed that their distribution was spatially biased. These results suggested that the disappearance of pore-free islands is highly related to de novo NPC assembly and indicated the existence of specific regulatory mechanisms for the spatial arrangement of NPC assembly on nuclear envelopes.

    DOI: 10.1038/s41598-017-16386-2

    PubMed

  • Crystal Structure and Characterization of Novel Human Histone H3 Variants, H3.6, H3.7, and H3.8 Reviewed

    Taguchi Hiroyuki, Xie Yan, Horikoshi Naoki, Maehara Kazumitsu, Harada Akihito, Nogami Jumpei, Sato Koichi, Arimura Yasuhiro, Osakabe Akihisa, Kujirai Tomoya, Iwasaki Takeshi, Semba Yuichiro, Tachibana Taro, Kimura Hiroshi, Ohkawa Yasuyuki, Kurumizaka Hitoshi

    BIOCHEMISTRY   56 ( 16 )   2184 - 2196   2017.04( ISSN:0006-2960

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/acs.biochem.6b01098

  • Crystal Structure and Characterization of Novel Human Histone H3 Variants, H3.6, H3.7, and H3.8 Reviewed

    Hiroyuki Taguchi, Yan Xie, Naoki Horikoshi, Kazumitsu Maehara, Akihito Harada, Jumpei Nogami, Koichi Sato, Yasuhiro Arimura, Akihisa Osakabe, Tomoya Kujirai, Takeshi Iwasaki, Yuichiro Semba, Taro Tachibana, Hiroshi Kimura, Yasuyuki Ohkawa, Hitoshi Kurumizaka

    BIOCHEMISTRY   56 ( 16 )   2184 - 2196   2017.04( ISSN:0006-2960

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    Publishing type:Research paper (scientific journal)  

    Non-allelic histone variants are considered as epigenetic factors that regulate genomic DNA functions in eukaryotic chromosomes. In this study, we identified three new human histone H3 variants (named H3.6, H3.7, and H3.8), which were previously annotated as pseudogenes. H3.6 and H3.8 conserve the H3.3-specific amino acid residues, but H3.7 shares the specific amino acid residues with H3.1. We successfully reconstituted the nucleosome containing H3.6 in vitro and determined its crystal structure. In the H3.6 nucleosome, the H3.6-specific Val62 residue hydrophobically contacts the cognate H4 molecule, but its contact area is smaller than that of the corresponding H3.3 Ile62 residue. The thermal stability assay revealed that the H3.6 nucleosome is substantially unstable, as compared to the, H3.3 nucleosome. Interestingly, mutational analysis demonstrated that the H3.6 Val62 residue is fully responsible for the H3.6 nucleosome instability, probably because of the weakened hydrophobic interaction with H4. We also reconstituted the nucleosome containing H3.8, but its thermal stability was quite low. In contrast, purified H3.7 failed to form nucleosomes in vitro. The identification and characterization of these novel human histone H3 variants provide important new insights into understanding the epigenetic regulation of the human genome.

    DOI: 10.1021/acs.biochem.6b01098

    PubMed

  • Septin 9_i2 is downregulated in tumors, impairs cancer cell migration and alters subnuclear actin filaments Reviewed

    Verdier-Pinard P., Salaun D., Bouguenina H., Shimada S., Pophillat M., Audebert S., Agavnian E., Coslet S., Charafe-Jauffret E., Tachibana T., Badache A.

    SCIENTIFIC REPORTS   7   2017.03( ISSN:2045-2322

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    DOI: 10.1038/srep44976

  • Flocculation of Real Sewage Sludge Using Poly-gamma-glutamic Acid Produced by <it>Bacillus</it> sp Isolated from Soil Reviewed

    Liu Tao, Yamashita Kyouhei, Fukumoto Yoshihiro, Tachibana Taro, Azuma Masayuki

    公益社団法人 化学工学会 JOURNAL OF CHEMICAL ENGINEERING OF JAPAN   50 ( 3 )   201 - 206   2017.03( ISSN:0021-9592

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    <p>To promote the use of sewage sludge on farmland, a flocculant compatible with agricultural application is required. In general, an inorganic flocculant such as aluminium sulfate is used at large sewage disposal plants. However, there is concern that aluminium accumulates when it is returned to farmland, and therefore a biodegradable rather than an inorganic flocculant is required. To obtain biodegradable flocculants produced by microorganisms, the latter were isolated from soils and sewage sludges, and the flocculation activity of the culture broths was evaluated. Here, real sewage sludge was used for the evaluation because practical use was regarded as an important consideration. As a result, two strains, V13 and RK14, were selected and identified as <i>Bacillus megaterium</i> and <i>Bacillus licheniformis</i>, respectively. It was also found that flocculation was caused by poly-γ-glutamic acid (γ-PGA). γ-PGA from RK14 cells had considerably higher molecular weight than commercial γ-PGA, and the enantiomeric composition differed from that of γ-PGA from other <i>B. licheniformis</i>. To obtain mutants with high γ-PGA productivity, RK14 cells were treated with ethyl methanesulfonate, and a mutant (RK14-46) was selected. The culture medium suitable for γ-PGA production by RK14-46 cells was totally different from that by RK-14 cells. The γ-PGA production reached 21.1 g/L when RK14-46 cells were cultured in the optimum semi-synthetic medium at 30°C for 2 d. Furthermore, the combination of γ-PGA obtained from RK14-46 cells and chitosan, a cationic flocculant, was very effective for flocculation of real sewage sludge.</p>

    DOI: 10.1252/jcej.16we158

    CiNii Article

  • Septin 9_i2 is downregulated in tumors, impairs cancer cell migration and alters subnuclear actin filaments Reviewed

    P. Verdier-Pinard, D. Salaun, H. Bouguenina, S. Shimada, M. Pophillat, S. Audebert, E. Agavnian, S. Coslet, E. Charafe-Jauffret, T. Tachibana, A. Badache

    SCIENTIFIC REPORTS   7   44976   2017.03( ISSN:2045-2322

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    Functions of septin cytoskeletal polymers in tumorigenesis are still poorly defined. Their role in the regulation of cytokinesis and cell migration were proposed to contribute to cancer associated aneuploidy and metastasis. Overexpression of Septin 9 (Sept9) promotes migration of cancer cell lines. SEPT9 mRNA and protein expression is increased in breast tumors compared to normal and peritumoral tissues and amplification of SEPT9 gene was positively correlated with breast tumor progression. However, the existence of multiple isoforms of Sept9 is a confounding factor in the analysis of Sept9 functions. In the present study, we analyze the protein expression of Sept9_i2, an uncharacterized isoform, in breast cancer cell lines and tumors and describe its specific impact on cancer cell migration and Sept9 cytoskeletal distribution. Collectively, our results showed that, contrary to Sept9_i1, Sept9_i2 did not support cancer cell migration, and induced a loss of subnuclear actin filaments. These effects were dependent on Sept9_i2 specific N-terminal sequence. Sept9_i2 was strongly down-regulated in breast tumors compared to normal mammary tissues. Thus our data indicate that Sept9_i2 is a negative regulator of breast tumorigenesis. We propose that Sept9 tumorigenic properties depend on the balance between Sept9_i1 and Sept9_i2 expression levels.

    DOI: 10.1038/srep44976

    PubMed

  • Application of Monoclonal Antibodies Against Mouse Dermokine Reviewed

    Kiyoshi Higashi, Cai-Xia Wang, Chikako Yokoyama, Keita Yamada, Koichi Saito, Taro Tachibana

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   36 ( 1 )   15 - 19   2017.02( ISSN:2167-9436

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    Dermokine is one of the most highly expressed proteins in differentiating keratinocytes. Mouse dermokine has been reported to be encoded by 22 exons, and its expression leads to three transcripts, β, γ, and α, which are transcribed from two different transcriptional start sites. The α isoform represents the carboxyl-terminal domain of the β isoform, whereas the γ isoform lacks this domain. To reveal the distributions and expression levels of each isoform in mice, we generated rat monoclonal antibodies against dermokine-β/γ and dermokine-β/α. In immunofluorescence studies, the expression levels of dermokine in the cytosol of the cultured mouse keratinocytes were significantly elevated by high levels of extracellular calcium. In Western blot analyses, the expression levels of dermokine-β and dermokine-α were increased in the presence of high calcium. Finally, we developed a monoclonal antibody-based sensitive sandwich enzyme-linked immunosorbent assay (ELISA) and showed that the secreted dermokine-β into the culture medium from mouse keratinocytes was significantly increased in a manner dependent on the extracellular calcium concentration. These dermokine-specific antibodies have allowed us to gain new insights into the role of each dermokine isoform in cutaneous homeostasis.

    DOI: 10.1089/mab.2016.0051

    PubMed

  • Application of Monoclonal Antibodies Against Mouse Dermokine. Reviewed

    Higashi K, Wang CX, Yokoyama C, Yamada K, Saito K, Tachibana T

    Monoclonal antibodies in immunodiagnosis and immunotherapy   36 ( 1 )   15 - 19   2017.02

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    DOI: 10.1089/mab.2016.0051

    PubMed

  • Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis Reviewed

    Ueda Jun, Harada Akihito, Urahama Takashi, Machida Shinichi, Maehara Kazumitsu, Hada Masashi, Makino Yoshinori, Nogami Jumpei, Horikoshi Naoki, Osakabe Akihisa, Taguchi Hiroyuki, Tanaka Hiroki, Tachiwana Hiroaki, Yao Tatsuma, Yamada Minami, Iwamoto Takashi, Isotani Ayako, Ikawa Masahito, Tachibana Taro, Okada Yuki, Kimura Hiroshi, Ohkawa Yasuyuki, Kurumizaka Hitoshi, Yamagata Kazuo

    CELL REPORTS   18 ( 3 )   593 - 600   2017.01( ISSN:2211-1247

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.celrep.2016.12.065

  • Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis Reviewed

    Jun Ueda, Akihito Harada, Takashi Urahama, Shinichi Machida, Kazumitsu Maehara, Masashi Hada, Yoshinori Makino, Jumpei Nogami, Naoki Horikoshi, Akihisa Osakabe, Hiroyuki Taguchi, Hiroki Tanaka, Hiroaki Tachiwana, Tatsuma Yao, Minami Yamada, Takashi Iwamoto, Ayako Isotani, Masahito Ikawa, Taro Tachibana, Yuki Okada, Hiroshi Kimura, Yasuyuki Ohkawa, Hitoshi Kurumizaka, Kazuo Yamagata

    CELL REPORTS   18 ( 3 )   593 - 600   2017.01( ISSN:2211-1247

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    Cellular differentiation is associated with dynamic chromatin remodeling in establishing a cell-typespecific epigenomic landscape. Here, we find that mouse testis-specific and replication-dependent histone H3 variant H3t is essential for very early stages of spermatogenesis. H3t gene deficiency leads to azoospermia because of the loss of haploid germ cells. When differentiating spermatogonia emerge in normal spermatogenesis, H3t appears and replaces the canonical H3 proteins. Structural and biochemical analyses reveal that H3t-containing nucleosomes are more flexible than the canonical nucleosomes. Thus, by incorporating H3t into the genome during spermatogonial differentiation, male germ cells are able to enter meiosis and beyond.

    DOI: 10.1016/j.celrep.2016.12.065

    PubMed

  • Flocculation of Real Sewage Sludge Using Poly-γ-glutamic Acid Produced by <i>Bacillus</i> sp. Isolated from Soil

    Liu Tao, Yamashita Kyouhei, Fukumoto Yoshihiro, Tachibana Taro, Azuma Masayuki

    JOURNAL OF CHEMICAL ENGINEERING OF JAPAN   50 ( 3 )   201 - 206   2017( ISSN:00219592

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    Publishing type:Research paper (scientific journal)  

    <p>To promote the use of sewage sludge on farmland, a flocculant compatible with agricultural application is required. In general, an inorganic flocculant such as aluminium sulfate is used at large sewage disposal plants. However, there is concern that aluminium accumulates when it is returned to farmland, and therefore a biodegradable rather than an inorganic flocculant is required. To obtain biodegradable flocculants produced by microorganisms, the latter were isolated from soils and sewage sludges, and the flocculation activity of the culture broths was evaluated. Here, real sewage sludge was used for the evaluation because practical use was regarded as an important consideration. As a result, two strains, V13 and RK14, were selected and identified as <i>Bacillus megaterium</i> and <i>Bacillus licheniformis</i>, respectively. It was also found that flocculation was caused by poly-γ-glutamic acid (γ-PGA). γ-PGA from RK14 cells had considerably higher molecular weight than commercial γ-PGA, and the enantiomeric composition differed from that of γ-PGA from other <i>B. licheniformis</i>. To obtain mutants with high γ-PGA productivity, RK14 cells were treated with ethyl methanesulfonate, and a mutant (RK14-46) was selected. The culture medium suitable for γ-PGA production by RK14-46 cells was totally different from that by RK-14 cells. The γ-PGA production reached 21.1 g/L when RK14-46 cells were cultured in the optimum semi-synthetic medium at 30°C for 2 d. Furthermore, the combination of γ-PGA obtained from RK14-46 cells and chitosan, a cationic flocculant, was very effective for flocculation of real sewage sludge.</p>

    DOI: 10.1252/jcej.16we158

    CiNii Article

  • ARHGEF10 directs the localization of Rab8 to Rab6-positive executive vesicles Reviewed

    Shibata Satoshi, Kawanai Tsubasa, Hara Takayuki, Yamamoto Asuka, Chaya Taro, Tokuhara Yasunori, Tsuji Chinami, Sakai Manabu, Tachibana Taro, Inagaki Shinobu

    JOURNAL OF CELL SCIENCE   129 ( 19 )   3620 - 3634   2016.10( ISSN:0021-9533

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1242/jcs.186817

  • ARHGEF10 directs the localization of Rab8 to Rab6-positive executive vesicles Reviewed

    Satoshi Shibata, Tsubasa Kawanai, Takayuki Hara, Asuka Yamamoto, Taro Chaya, Yasunori Tokuhara, Chinami Tsuji, Manabu Sakai, Taro Tachibana, Shinobu Inagaki

    JOURNAL OF CELL SCIENCE   129 ( 19 )   3620 - 3634   2016.10( ISSN:0021-9533

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    Publishing type:Research paper (scientific journal)  

    The function of ARHGEF10, a known guanine nucleotide exchange factor (GEF) for RhoA with proposed roles in various diseases, is poorly understood. To understand the precise function of this protein, we raised a monoclonal antibody against ARHGEF10 and determined its localization in HeLa cells. ARHGEF10 was found to localize to vesicles containing Rab6 (of which there are three isoforms, Rab6a, Rab6b and Rab6c), Rab8 (of which there are two isoforms, Rab8a and Rab8b), and/or the secretion marker neuropeptide Y (NPY)-Venus in a Rab6-dependent manner. These vesicles were known to originate from the Golgi and contain secreted or membrane proteins. Ectopic expression of an N-terminal-truncated ARHGEF10 mutant led to the generation of large vesicle-like structures containing both Rab6 and Rab8. Additionally, small interfering (si) RNA-mediated knockdown of ARHGEF10 impaired the localization of Rab8 to these exocytotic vesicles. Furthermore, the invasiveness of MDA-MB231 cells was markedly decreased by knockdown of ARHGEF10, as well as of Rab8. From these results, we propose that ARHGEF10 acts in exocytosis and tumor invasion in a Rab8-dependent manner.

    DOI: 10.1242/jcs.186817

    PubMed

  • The wound/burn guidelines - 6: Guidelines for the management of burns. Reviewed

    Yoshino Y, Ohtsuka M, Kawaguchi M, Sakai K, Hashimoto A, Hayashi M, Madokoro N, Asano Y, Abe M, Ishii T, Isei T, Ito T, Inoue Y, Imafuku S, Irisawa R, Ohtsuka M, Ogawa F, Kadono T, Kawakami T, Kukino R, Kono T, Kodera M, Takahara M, Tanioka M, Nakanishi T, Nakamura Y, Hasegawa M, Fujimoto M, Fujiwara H, Maekawa T, Matsuo K, Yamasaki O, Le Pavoux A, Tachibana T, Ihn H, Wound/Burn Guidelines Committee

    The Journal of dermatology   43 ( 9 )   989 - 1010   2016.09( ISSN:0385-2407

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    DOI: 10.1111/1346-8138.13288

    PubMed

  • The wound/burn guidelines - 5: Guidelines for the management of lower leg ulcers/varicose veins. Reviewed

    Ito T, Kukino R, Takahara M, Tanioka M, Nakamura Y, Asano Y, Abe M, Ishii T, Isei T, Inoue Y, Imafuku S, Irisawa R, Ohtsuka M, Ohtsuka M, Ogawa F, Kadono T, Kawakami T, Kawaguchi M, Kono T, Kodera M, Sakai K, Nakanishi T, Hashimoto A, Hasegawa M, Hayashi M, Fujimoto M, Fujiwara H, Maekawa T, Matsuo K, Madokoro N, Yamasaki O, Yoshino Y, Le Pavoux A, Tachibana T, Ihn H, Wound/Burn Guidelines Committee

    The Journal of dermatology   43 ( 8 )   853 - 68   2016.08( ISSN:0385-2407

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/1346-8138.13286

    PubMed

  • The wound/burn guidelines - 4: Guidelines for the management of skin ulcers associated with connective tissue disease/vasculitis. Reviewed

    Fujimoto M, Asano Y, Ishii T, Ogawa F, Kawakami T, Kodera M, Abe M, Isei T, Ito T, Inoue Y, Imafuku S, Irisawa R, Ohtsuka M, Ohtsuka M, Kadono T, Kawaguchi M, Kukino R, Kono T, Sakai K, Takahara M, Tanioka M, Nakanishi T, Nakamura Y, Hashimoto A, Hasegawa M, Hayashi M, Fujiwara H, Maekawa T, Matsuo K, Madokoro N, Yamasaki O, Yoshino Y, Le Pavoux A, Tachibana T, Ihn H, Wound/Burn Guidelines Committee

    The Journal of dermatology   43 ( 7 )   729 - 57   2016.07( ISSN:0385-2407

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/1346-8138.13275

    PubMed

  • Generation of Rat Monoclonal Antibodies Against Human Pancreatic Ductal Adenocarcinoma Cells Reviewed

    Kiyoshi Higashi, Nobuaki Fujii, Masahiko Kushida, Keita Yamada, Noriyuki Suzuki, Koichi Saito, Taro Tachibana

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   35 ( 3 )   148 - 154   2016.06( ISSN:2167-9436

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    Pancreatic ductal adenocarcinoma is an aggressive tumor with a poor prognosis. Biomarkers that can detect the tumor in its early stages when it may be amenable to curative resection might improve prognosis. To discover novel markers expressed in primary pancreatic cancer, we generated a panel of monoclonal antibodies against pancreatic ductal adenocarcinoma cell line BxPC3 using a rat medial iliac lymph node method. The antigen recognized by 1B5A5 was expressed on the cell surface and secreted into the conditioned medium of BxPC3 cells, and characterized as glycoproteins with molecular mass between 60 and 90 kDa. A wide range of molecular weights of 1B5A5 antigen in several pancreatic cancer cell lines were observed. Immunohistochemistry using a human multiple organ tumor tissue array showed an enhanced expression of 1B5A5 antigen in pancreas, lung, stomach, breast, urinary bladder, colon, and cervix uteri cancers. Immunoprecipitation followed by proteomic analyses identified CEACAM6 as a 1B5A5 antigen. In addition, western blot analysis results indicated that the 1B5A5 epitope is located within an amino-terminal domain of CEACAM6. These results raised the possibility that our approach could lead to discovery of novel biomarkers for the early stage of cancers in a relatively short period of time.

    DOI: 10.1089/mab.2016.0012

    PubMed

  • Generation of Rat Monoclonal Antibodies Against Human Pancreatic Ductal Adenocarcinoma Cells. Reviewed

    Higashi K, Fujii N, Kushida M, Yamada K, Suzuki N, Saito K, Tachibana T

    Monoclonal antibodies in immunodiagnosis and immunotherapy   35 ( 3 )   148 - 54   2016.06

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/mab.2016.0012

    PubMed

  • The wound/burn guidelines - 3: Guidelines for the diagnosis and treatment for diabetic ulcer/gangrene. Reviewed

    Isei T, Abe M, Nakanishi T, Matsuo K, Yamasaki O, Asano Y, Ishii T, Ito T, Inoue Y, Imafuku S, Irisawa R, Ohtsuka M, Ohtsuka M, Ogawa F, Kadono T, Kodera M, Kawakami T, Kawaguchi M, Kukino R, Kono T, Sakai K, Takahara M, Tanioka M, Nakamura Y, Hashimoto A, Hasegawa M, Hayashi M, Fujimoto M, Fujiwara H, Maekawa T, Madokoro N, Yoshino Y, Le Pavoux A, Tachibana T, Ihn H, Wound/Burn Guidelines Committee

    The Journal of dermatology   43 ( 6 )   591 - 619   2016.06( ISSN:0385-2407

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    DOI: 10.1111/1346-8138.13285

    PubMed

  • The wound/burn guidelines - 2: Guidelines for the diagnosis and treatment for pressure ulcers. Reviewed

    Tachibana T, Imafuku S, Irisawa R, Ohtsuka M, Kadono T, Fujiwara H, Asano Y, Abe M, Ishii T, Isei T, Ito T, Inoue Y, Ohtsuka M, Ogawa F, Kodera M, Kawakami T, Kawaguchi M, Kukino R, Kono T, Sakai K, Takahara M, Tanioka M, Nakanishi T, Nakamura Y, Hashimoto A, Hasegawa M, Hayashi M, Fujimoto M, Maekawa T, Matsuo K, Madokoro N, Yamasaki O, Yoshino Y, Le Pavoux A, Ihn H, Wound/Burn Guidelines Committee

    The Journal of dermatology   43 ( 5 )   469 - 506   2016.05( ISSN:0385-2407

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/1346-8138.13274

    PubMed

  • The wound/burn guidelines - 1: Wounds in general. Reviewed

    Inoue Y, Hasegawa M, Maekawa T, Le Pavoux A, Asano Y, Abe M, Ishii T, Ito T, Isei T, Imafuku S, Irisawa R, Ohtsuka M, Ohtsuka M, Ogawa F, Kadono T, Kodera M, Kawakami T, Kawaguchi M, Kukino R, Kono T, Sakai K, Takahara M, Tanioka M, Nakanishi T, Nakamura Y, Hashimoto A, Hayashi M, Fujimoto M, Fujiwara H, Matsuo K, Madokoro N, Yamasaki O, Yoshino Y, Tachibana T, Ihn H, Wound/Burn Guidelines Committee

    The Journal of dermatology   43 ( 4 )   357 - 75   2016.04( ISSN:0385-2407

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    DOI: 10.1111/1346-8138.13276

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  • Chromatin-prebound Crm1 recruits Nup98-HoxA9 fusion to induce aberrant expression of Hox cluster genes Reviewed

    Oka Masahiro, Mura Sonoko, Yamada Kohji, Sangel Percival, Hirata Saki, Maehara Kazumitsu, Kawakami Koichi, Tachibana Taro, Ohkawa Yasuyuki, Kimura Hiroshi, Yoneda Yoshihiro

    ELIFE   5   2016.01( ISSN:2050-084X

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    DOI: 10.7554/eLife.09540

  • Chromatin-prebound Crm1 recruits Nup98-HoxA9 fusion to induce aberrant expression of Hox cluster genes Reviewed

    Masahiro Oka, Sonoko Mura, Kohji Yamada, Percival Sangel, Saki Hirata, Kazumitsu Maehara, Koichi Kawakami, Taro Tachibana, Yasuyuki Ohkawa, Hiroshi Kimura, Yoshihiro Yoneda

    ELIFE   5   e09540   2016.01( ISSN:2050-084X

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    The nucleoporin Nup98 is frequently rearranged to form leukemogenic Nup98-fusion proteins with various partners. However, their function remains largely elusive. Here, we show that Nup98-HoxA9, a fusion between Nup98 and the homeobox transcription factor HoxA9, forms nuclear aggregates that frequently associate with facultative heterochromatin. We demonstrate that stable expression of Nup98-HoxA9 in mouse embryonic stem cells selectively induces the expression of Hox cluster genes. Genome-wide binding site analysis revealed that Nup98-HoxA9 is preferentially targeted and accumulated at Hox cluster regions where the export factor Crm1 is originally prebound. In addition, leptomycin B, an inhibitor of Crm1, disassembled nuclear Nup98-HoxA9-dots, resulting in the loss of chromatin binding of Nup98-HoxA9 and Nup98-HoxA9mediated activation of Hox genes. Collectively, our results indicate that highly selective targeting of Nup98-fusion proteins to Hox cluster regions via prebound Crm1 induces the formation of higher order chromatin structures that causes aberrant Hox gene regulation.

    DOI: 10.7554/eLife.09540

    PubMed

  • Generation and characterization of rat monoclonal antibodies against epidermal growth factor receptor Reviewed

    Tomohiro Osaki, Cai-Xia Wang, Taro Tachibana, Masayuki Azuma, Masaya Kitamura, Takeshi Nakanishi

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   34 ( 6 )   418 - 422   2015.12( ISSN:2167-9436

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    Overexpression of the epidermal growth factor receptor (EGFR) gene and dysregulation of EGFR signaling are observed in various cancer cells, and EGFR is a validated target for cancer therapy. In the present study, we report on the generation of two rat anti-EGFR antibodies (clones 2C2D3 and 4H7F4) by using the rat lymph node method. Flow cytometric analysis and immunofluorescence showed that both antibodies specifically bound to EGFR on the surface of cancer cells. Competitive analysis demonstrated that the epitope of each antibody had no overlap with that of the therapeutic anti-EGFR antibody cetuximab. These results suggest that 2C2D3 and 4H7F4 are potentially useful in EGFR-targeted cancer therapy.

    DOI: 10.1089/mab.2015.0034

    PubMed

  • Generation and Characterization of Rat Monoclonal Antibodies Against Epidermal Growth Factor Receptor. Reviewed

    Osaki T, Wang CX, Tachibana T, Azuma M, Kitamura M, Nakanishi T

    Monoclonal antibodies in immunodiagnosis and immunotherapy   34 ( 6 )   418 - 22   2015.12

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    DOI: 10.1089/mab.2015.0034

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  • Phosphorylation of myosin II regulatory light chain by ZIP kinase is responsible for cleavage furrow ingression during cell division in mammalian cultured cells Reviewed

    Hosoba Kosuke, Komatsu Satoshi, Ikebe Mitsuo, Kotani Manato, Xiao Wenqin, Tachibana Taro, Hosoya Hiroshi, Hamao Kozue

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   459 ( 4 )   686 - 691   2015.04( ISSN:0006-291X

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    DOI: 10.1016/j.bbrc.2015.03.005

  • Nuclear import of the U1A splicesome protein is mediated by importin α/β and Ran in living mammalian cells. Reviewed

    Hieda M, Tachibana T, Fukumoto M, Yoneda Y

    The Journal of biological chemistry   290 ( 16 )   9948   2015.04( ISSN:0021-9258

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    DOI: 10.1074/jbc.A115.008299

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  • Nuclear import of the U1A splicesome protein is mediated by importin alpha/beta and Ran in living mammalian cells. (vol 276, pg 16824, 2001) Reviewed

    Miki Hieda, Taro Tachibana, Masahiro Fukumoto, Yoshihiro Yoneda

    JOURNAL OF BIOLOGICAL CHEMISTRY   290 ( 16 )   9948 - 9948   2015.04( ISSN:0021-9258

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    DOI: 10.1074/jbc.A115.008299

    PubMed

  • Phosphorylation of myosin II regulatory light chain by ZIP kinase is responsible for cleavage furrow ingression during cell division in mammalian cultured cells Reviewed

    Kosuke Hosoba, Satoshi Komatsu, Mitsuo Ikebe, Manato Kotani, Xiao Wenqin, Taro Tachibana, Hiroshi Hosoya, Kozue Hamao

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   459 ( 4 )   686 - 691   2015.04( ISSN:0006-291X

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    Zipper-interacting protein kinase (ZIPK) is known to regulate several functions such as apoptosis, smooth muscle contraction, and cell migration. While exogenously expressed GFP-ZIPK localizes to the cleavage furrow, role of ZIPK in cytokinesis is obscure. Here, we show that ZIPK is a major MRLC kinase during mitosis. Moreover, ZIPK siRNA-mediated knockdown causes delay of cytokinesis. The delay in cytokinesis of ZINC-knockdown cells was rescued by the exogenous diphosphorylation-mimicking MRLC mutant. Taken together, these findings suggest that ZIPK plays a role in the progression and completion of cytokinesis through MRLC phosphorylation. (C) 2015 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2015.03.005

    PubMed

  • DBZ Regulates Cortical Cell Positioning and Neurite Development by Sustaining the Anterograde Transport of Lis1 and DISC1 through Control of Ndel1 Dual-Phosphorylation Reviewed

    Okamoto Masayuki, Iguchi Tokuichi, Hattori Tsuyoshi, Matsuzaki Shinsuke, Koyama Yoshihisa, Taniguchi Manabu, Komada Munekazu, Xie Min-Jue, Yagi Hideshi, Shimizu Shoko, Konishi Yoshiyuki, Omi Minoru, Yoshimi Tomohiko, Tachibana Taro, Fujieda Shigeharu, Katayama Taiichi, Ito Akira, Hirotsune Shinji, Tohyama Masaya, Sato Makoto

    JOURNAL OF NEUROSCIENCE   35 ( 7 )   2942 - 2958   2015.02( ISSN:0270-6474

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1523/JNEUROSCI.5029-13.2015

  • A Novel Marker for Undifferentiated Human Embryonic Stem Cells Reviewed

    Kiyoshi Higashi, Masaki Yagi, Tatsuhiko Arakawa, Kouji Asano, Kumiko Kobayashi, Taro Tachibana, Koichi Saito

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   34 ( 1 )   7 - 11   2015.02( ISSN:2167-9436

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    Human embryonic stem cells (hESCs) are pluripotent stem cells from early embryos, and their self-renewal capacity depends on the sustained expression of hESC-specific molecules and the suppressed expression of differentiation-associated genes. To discover novel molecules expressed on hESCs, we generated a panel of monoclonal antibodies against undifferentiated hESCs. The antigen recognized by MAb2 is expressed on the cell surface of undifferentiated hESCs
    three diffused bands with molecular mass between 30 and 60kDa in the lysates of hESCs were diminished during hESC differentiation into neural cells. The expression of MAb2 antigen was also observed on the plasma membrane of lung cancer cells, and MAb2 detected 55, 50, and 35kDa protein bands in the cell lysates. Immunoprecipitation followed by proteomics analyses identified CD147/basigin as a MAb2 antigen. Finally, the positive expression of CD147/basigin protein in undifferentiated hESCs was confirmed. These results suggested that CD147/basigin could be another undifferentiated hESC marker.

    DOI: 10.1089/mab.2014.0075

    PubMed

  • A novel marker for undifferentiated human embryonic stem cells. Reviewed

    Higashi K, Yagi M, Arakawa T, Asano K, Kobayashi K, Tachibana T, Saito K

    Monoclonal antibodies in immunodiagnosis and immunotherapy   34 ( 1 )   7 - 11   2015.02

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    DOI: 10.1089/mab.2014.0075

    PubMed

  • DBZ Regulates Cortical Cell Positioning and Neurite Development by Sustaining the Anterograde Transport of Lis1 and DISC1 through Control of Ndel1 Dual-Phosphorylation Reviewed

    Masayuki Okamoto, Tokuichi Iguchi, Tsuyoshi Hattori, Shinsuke Matsuzaki, Yoshihisa Koyama, Manabu Taniguchi, Munekazu Komada, Min-Jue Xie, Hideshi Yagi, Shoko Shimizu, Yoshiyuki Konishi, Minoru Omi, Tomohiko Yoshimi, Taro Tachibana, Shigeharu Fujieda, Taiichi Katayama, Akira Ito, Shinji Hirotsune, Masaya Tohyama, Makoto Sato

    JOURNAL OF NEUROSCIENCE   35 ( 7 )   2942 - 2958   2015.02( ISSN:0270-6474

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    Cell positioning and neuronal network formation are crucial for proper brain function. Disrupted-in-Schizophrenia 1 (DISC1) is anterogradely transported to the neurite tips, together with Lis1, and functions in neurite extension via suppression of GSK3 beta activity. Then, transported Lis1 is retrogradely transported and functions in cell migration. Here, we show that DISC1-binding zinc finger protein (DBZ), together with DISC1, regulates mouse cortical cell positioning and neurite development in vivo. DBZ hindered Ndel1 phosphorylation at threonine 219 and serine 251. DBZ depletion or expression of a double-phosphorylated mimetic form of Ndel1 impaired the transport of Lis1 and DISC1 to the neurite tips and hampered microtubule elongation. Moreover, application of DISC1 or a GSK3 beta inhibitor rescued the impairments caused by DBZ insufficiency or double-phosphorylated Ndel1 expression. We concluded that DBZ controls cell positioning and neurite development by interfering with Ndel1 from disproportionate phosphorylation, which is critical for appropriate anterograde transport of the DISC1-complex.

    DOI: 10.1523/JNEUROSCI.5029-13.2015

    PubMed

  • Incorporation of histone H3.1 suppresses the lineage potential of skeletal muscle Reviewed

    Harada Akihito, Maehara Kazumitsu, Sato Yuko, Konno Daijiro, Tachibana Taro, Kimura Hiroshi, Ohkawa Yasuyuki

    NUCLEIC ACIDS RESEARCH   43 ( 2 )   775 - 786   2015.01( ISSN:0305-1048

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    DOI: 10.1093/nar/gku1346

  • Incorporation of histone H3.1 suppresses the lineage potential of skeletal muscle Reviewed

    Akihito Harada, Kazumitsu Maehara, Yuko Sato, Daijiro Konno, Taro Tachibana, Hiroshi Kimura, Yasuyuki Ohkawa

    NUCLEIC ACIDS RESEARCH   43 ( 2 )   775 - 786   2015.01( ISSN:0305-1048

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    Lineage potential is triggered by lineage-specific transcription factors in association with changes in the chromatin structure. Histone H3.3 variant is thought to play an important role in the regulation of lineage-specific genes. To elucidate the function of H3.3 in myogenic differentiation, we forced the expression of GFP-H3.1 to alter the balance between H3.1 and H3.3 in mouse C2C12 cells that could be differentiated intomyotubes. GFP-H3.1 replaced H3.3 in the regulatory regions of skeletal muscle (SKM) genes and induced a decrease of H3K4 trimethylation (H3K4me3) and increase of H3K27 trimethylation (H3K27me3). Similar results were obtained by H3.3 knockdown. In contrast, MyoD-dependent H3.3 incorporation into SKM genes in fibroblasts induced an increase of H3K4me3 and H3K27me3. In mouse embryos, a bivalent modification of H3K4me3 and H3K27me3 was formed on H3.3-incorporated SKM genes before embryonic skeletal muscle differentiation. These results suggest that lineage potential is established through a selective incorporation of specific H3 variants that governs the balance of histone modifications.

    DOI: 10.1093/nar/gku1346

    PubMed

  • Expression of the Clustered NeuAc alpha 2-3Gal beta O-Glycan Determines the Cell Differentiation State of the Cells Reviewed

    Higashi Kiyoshi, Asano Kouji, Yagi Masaki, Yamada Keita, Arakawa Tatsuhiko, Ehashi Tomo, Mori Takashi, Sumida Kayo, Kushida Masahiko, Ando Satoshi, Kinoshita Mitsuhiro, Kakehi Kazuaki, Tachibana Taro, Saito Koichi

    JOURNAL OF BIOLOGICAL CHEMISTRY   289 ( 37 )   25833 - 25843   2014.09( ISSN:0021-9258

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    DOI: 10.1074/jbc.M114.550848

  • Expression of the Clustered NeuAc alpha 2-3Gal beta O-Glycan Determines the Cell Differentiation State of the Cells Reviewed

    Kiyoshi Higashi, Kouji Asano, Masaki Yagi, Keita Yamada, Tatsuhiko Arakawa, Tomo Ehashi, Takashi Mori, Kayo Sumida, Masahiko Kushida, Satoshi Ando, Mitsuhiro Kinoshita, Kazuaki Kakehi, Taro Tachibana, Koichi Saito

    JOURNAL OF BIOLOGICAL CHEMISTRY   289 ( 37 )   25833 - 25843   2014.09( ISSN:0021-9258

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    Human embryonic stem cells (hESCs) are pluripotent stem cells from early embryos, and their self-renewal capacity depends on the sustained expression of hESC-specific molecules and the suppressed expression of differentiation-associated genes. To discover novel molecules expressed on hESCs, we generated a panel of monoclonal antibodies against undifferentiated hESCs and evaluated their ability to mark cancer cells, as well as hESCs. MAb7 recognized undifferentiated hESCs and showed a diffuse band with molecular mass of &gt; 239 kDa in the lysates of hESCs. Although some amniotic epithelial cells expressed MAb7 antigen, its expression was barely detected in normal human keratinocytes, fibroblasts, or endothelial cells. The expression of MAb7 antigen was observed only in pancreatic and gastric cancer cells, and its levels were elevated in metastatic and poorly differentiated cancer cell lines. Analyses of MAb7 antigen suggested that the clustered NeuAc alpha 2-3Gal beta O-linked oligosaccharides on DMBT1 (deleted in malignant brain tumors 1) were critical for MAb7 binding in cancer cells. Although features of MAb7 epitope were similar with those of TRA-1-60, distribution of MAb7 antigen in cancer cells was different from that of TRA-1-60 antigen. Exposure of a histone deacetylase inhibitor to differentiated gastric cancer MKN74 cells evoked the expression of MAb7 antigen, whereas DMBT1 expression remained unchanged. Cell sorting followed by DNA microarray analyses identified the down-regulated genes responsible for the biosynthesis of MAb7 antigen in MKN74 cells. In addition, treatment of metastatic pancreatic cancer cells with MAb7 significantly abrogated the adhesion to endothelial cells. These results raised the possibility that MAb7 epitope is a novel marker for undifferentiated cells such as hESCs and cancer stem-like cells and plays a possible role in the undifferentiated cells.

    DOI: 10.1074/jbc.M114.550848

    PubMed

  • N-ethylmaleimide-sensitive factor interacts with the serotonin transporter and modulates its trafficking: implications for pathophysiology in autism Reviewed

    Iwata Keiko, Matsuzaki Hideo, Tachibana Taro, Ohno Koji, Yoshimura Saori, Takamura Hironori, Yamada Kohei, Matsuzaki Shinsuke, Nakamura Kazuhiko, Tsuchiya Kenji J., Matsumoto Kaori, Tsujii Masatsugu, Sugiyama Toshirou, Katayama Taiichi, Mori Norio

    MOLECULAR AUTISM   5   2014.05( ISSN:2040-2392

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    DOI: 10.1186/2040-2392-5-33

  • KRAS mutation confers resistance to antibody-dependent cellular cytotoxicity of cetuximab against human colorectal cancer cells Reviewed

    Nakadate Yusuke, Kodera Yasuo, Kitamura Yuka, Shirasawa Senji, Tachibana Taro, Tamura Tomohide, Koizumi Fumiaki

    INTERNATIONAL JOURNAL OF CANCER   134 ( 9 )   2146 - 2155   2014.05( ISSN:0020-7136

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    DOI: 10.1002/ijc.28550

  • DBZ, a CNS- Specific DISC1 Binding Protein, Positively Regulates Oligodendrocyte Differentiation Reviewed

    Shimizu Shoko, Koyama Yoshihisa, Hattori Tsuyoshi, Tachibana Taro, Yoshimi Tomohiko, Emoto Hisayo, Matsumoto Yuji, Miyata Shingo, Katayama Taiichi, Ito Akira, Tohyama Masaya

    GLIA   62 ( 5 )   709 - 724   2014.05( ISSN:0894-1491

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    DOI: 10.1002/glia.22636

  • Isolation of diploid baker's yeast capable of strongly activating immune cells and analyses of the cell wall structure Reviewed

    Takada Yuki, Mizobuchi Ayano, Kato Takayuki, Kasahara Emiko, Ito Chinatsu, Watanabe Hajime, Kanzaki Ken, Kitagawa Seiichi, Tachibana Taro, Azuma Masayuki

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   78 ( 5 )   911 - 915   2014.05( ISSN:0916-8451

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    DOI: 10.1080/09168451.2014.917259

  • DBZ, a CNS- Specific DISC1 Binding Protein, Positively Regulates Oligodendrocyte Differentiation Reviewed

    Shoko Shimizu, Yoshihisa Koyama, Tsuyoshi Hattori, Taro Tachibana, Tomohiko Yoshimi, Hisayo Emoto, Yuji Matsumoto, Shingo Miyata, Taiichi Katayama, Akira Ito, Masaya Tohyama

    GLIA   62 ( 5 )   709 - 724   2014.05( ISSN:0894-1491

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    Recent studies have shown changes in myelin genes and alterations in white matter structure in a wide range of psychiatric disorders. Here we report that DBZ, a central nervous system (CNS)-specific member of the DISC1 interactome, positively regulates the oligodendrocyte (OL) differentiation in vivo and in vitro. In mouse corpus callosum (CC), DBZ mRNA is expressed in OL lineage cells and expression of DBZ protein peaked before MBP expression. In the CC of DBZ-KO mice, we observed delayed myelination during the early postnatal period. Although the myelination delay was mostly recovered by adulthood, OLs with immature structural features were more abundant in adult DBZ-KO mice than in control mice. DBZ was also transiently upregulated during rat OL differentiation in vitro before myelin marker expression. DBZ knockdown by RNA interference resulted in a decreased expression of myelin-related markers and a low number of cells with mature characteristics, but with no effect on the proliferation of oligodendrocyte precursor cells. We also show that the expression levels of transcription factors having a negative-regulatory role in OL differentiation were upregulated when endogenous DBZ was knocked down. These results strongly indicate that OL differentiation in rodents is regulated by DBZ.

    DOI: 10.1002/glia.22636

  • 免疫細胞を強力に活性化できる2倍体パン用イーストの分離およびその細胞壁構造の解析(Isolation of diploid baker's yeast capable of strongly activating immune cells and analyses of the cell wall structure) Reviewed

    Takada Yuki, Mizobuchi Ayano, Kato Takayuki, Kasahara Emiko, Ito Chinatsu, Watanabe Hajime, Kanzaki Ken, Kitagawa Seiichi, Tachibana Taro, Azuma Masayuki

    Bioscience, Biotechnology, and Biochemistry   78 ( 5 )   911 - 915   2014.05( ISSN:0916-8451

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    ハプロイド突然変異体AQ-37に基づいて、マウスマクロファージを強力に活性化できる2倍体パン用イーストを構築し、その活性化に対する要因を検討した。得られた3種の2倍体株(BQ-10、BQ-48、BQ-55)のうち、BQ-55が最高のマクロファージ活性化能を示したため、2倍体パン用イーストとして詳細な評価を行った。BQ-55の増殖能はAQ-37より約5倍高かった。2倍体株BQ-55はヒト免疫細胞も活性化した。マウスRAW264.7細胞およびヒト末梢血単核球細胞において、BQ-55はTNF-α分泌を有意に促進した。この活性化に対する要因を解明するため、その細胞壁構造、特にβグルカン構造を解析した結果、分岐の長さ、β-1,6-グルカンがその要因の一つであることが示唆された。

  • N-ethylmaleimide-sensitive factor interacts with the serotonin transporter and modulates its trafficking: implications for pathophysiology in autism Reviewed

    Keiko Iwata, Hideo Matsuzaki, Taro Tachibana, Koji Ohno, Saori Yoshimura, Hironori Takamura, Kohei Yamada, Shinsuke Matsuzaki, Kazuhiko Nakamura, Kenji J. Tsuchiya, Kaori Matsumoto, Masatsugu Tsujii, Toshirou Sugiyama, Taiichi Katayama, Norio Mori

    MOLECULAR AUTISM   5   33   2014.05( ISSN:2040-2392

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    Background: Changes in serotonin transporter (SERT) function have been implicated in autism. SERT function is influenced by the number of transporter molecules present at the cell surface, which is regulated by various cellular mechanisms including interactions with other proteins. Thus, we searched for novel SERT-binding proteins and investigated whether the expression of one such protein was affected in subjects with autism.
    Methods: Novel SERT-binding proteins were examined by a pull-down system. Alterations of SERT function and membrane expression upon knockdown of the novel SERT-binding protein were studied in HEK293-hSERT cells. Endogenous interaction of SERT with the protein was evaluated in mouse brains. Alterations in the mRNA expression of SERT (SLC6A4) and the SERT-binding protein in the post-mortem brains and the lymphocytes of autism patients were compared to nonclinical controls.
    Results: N-ethylmaleimide-sensitive factor (NSF) was identified as a novel SERT-binding protein. NSF was co-localized with SERT at the plasma membrane, and NSF knockdown resulted in decreased SERT expression at the cell membranes and decreased SERT uptake function. NSF was endogenously co-localized with SERT and interacted with SERT. While SLC6A4 expression was not significantly changed, NSF expression tended to be reduced in post-mortem brains, and was significantly reduced in lymphocytes of autistic subjects, which correlated with the severity of the clinical symptoms.
    Conclusions: These data clearly show that NSF interacts with SERT under physiological conditions and is required for SERT membrane trafficking and uptake function. A possible role for NSF in the pathophysiology of autism through modulation of SERT trafficking, is suggested.

    DOI: 10.1186/2040-2392-5-33

    PubMed

  • KRAS mutation confers resistance to antibody-dependent cellular cytotoxicity of cetuximab against human colorectal cancer cells Reviewed

    Yusuke Nakadate, Yasuo Kodera, Yuka Kitamura, Senji Shirasawa, Taro Tachibana, Tomohide Tamura, Fumiaki Koizumi

    INTERNATIONAL JOURNAL OF CANCER   134 ( 9 )   2146 - 2155   2014.05( ISSN:0020-7136

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    Cetuximab is a chimeric IgG1 monoclonal antibody (mAb) that targets the extracellular domain of epidermal growth factor receptor (EGFR). Oncogenic KRAS mutations in tumors have been shown to be a negative predictor of the response of colorectal cancer (CRC) to cetuximab treatment. Cetuximab exerts its therapeutic effects through several mechanisms including antibody-dependent cellular cytotoxicity (ADCC). However, the influence of KRAS mutations on cetuximab-mediated ADCC is not fully understood. Here, we investigated cetuximab-mediated ADCC in two pairs of isogenic CRC cells with or without a KRAS mutation. Peripheral blood mononuclear cells (PBMCs) from healthy volunteers and NK92, a natural killer (NK) cell line that exogenously expresses Fc?RIIIa (CD16a), were used as effector cells. In an ADCC assay, perforin-dependent target cell lysis was not affected by the KRAS mutation status. On the other hand, perforin-independent ADCC was observed only in CRC cells with wild-type KRAS, but not in cells with mutant KRAS. Neutralizing experiments revealed that the Fas-Fas ligand (FasL) interaction was responsible for the induction of apoptosis and perforin-independent ADCC. Furthermore, the presence of effector cells clearly enhanced the growth-inhibitory effect of cetuximab only in CRC cells with wild-type KRAS, but not in those with mutant KRAS. These findings suggest that ADCC is an important mode of action of cetuximab and that KRAS mutation impairs the therapeutic effect exerted by cetuximab-mediated ADCC.

    DOI: 10.1002/ijc.28550

  • Isolation of diploid baker's yeast capable of strongly activating immune cells and analyses of the cell wall structure Reviewed

    Yuki Takada, Ayano Mizobuchi, Takayuki Kato, Emiko Kasahara, Chinatsu Ito, Hajime Watanabe, Ken Kanzaki, Seiichi Kitagawa, Taro Tachibana, Masayuki Azuma

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   78 ( 5 )   911 - 915   2014.05( ISSN:0916-8451

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    Diploid baker's yeast capable of strongly activating a mouse macrophage was constructed based on haploid mutant AQ-37 obtained previously. The obtained strain BQ-55 activated also human immune cells. To clarify a factor for the activation, the cell wall structure, especially the beta-glucan structure, was analyzed, suggesting that the length of branching, beta-1,6-glucan, may be one of the factors.

    DOI: 10.1080/09168451.2014.917259

    PubMed

  • Generation of a monoclonal antibody against the extracellular domain of IGF-1R Reviewed

    Yusuke Nakadate, Saori Yoshimura, Naoko Nakatani, Masayuki Azuma, Taro Tachibana

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   33 ( 2 )   126 - 128   2014.04( ISSN:2167-9436

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    Type I insulin-like growth factor receptor (IGF-1R) is a receptor tyrosine kinase that is involved in the transformation of cells, cancer proliferation, and metastatic events in various types of cancer. The present study reports on the generation of a mouse monoclonal antibody (MAb) to IGF-1R using the mouse lymph node method. MAb 1B1, which reacts specifically with the extracellular domain of IGF-1R, was obtained using flow cytometry (FCM) screening using MCF-7 cells. Using immunostaining, MAb 1B1 detected IGF-1R mainly on the plasma membrane of MCF-7 cells. MAb 1B1 would be useful in FCM and immunofluorescence assays to detect IGF-1R-expressing cells for basic and clinical research. © Copyright 2014, Mary Ann Liebert, Inc. 2014.

    DOI: 10.1089/mab.2013.0083

    PubMed

  • Generation of a monoclonal antibody against the extracellular domain of IGF-1R. Reviewed

    Nakadate Y, Yoshimura S, Nakatani N, Azuma M, Tachibana T

    Monoclonal antibodies in immunodiagnosis and immunotherapy   33 ( 2 )   126 - 8   2014.04

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    DOI: 10.1089/mab.2013.0083

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  • Isolation and characterization of baker's yeast capable of strongly activating a macrophage Reviewed

    Takada Yuki, Nishino Yumiko, Ito Chinatsu, Watanabe Hajime, Kanzaki Ken, Tachibana Taro, Azuma Masayuki

    FEMS YEAST RESEARCH   14 ( 2 )   261 - 269   2014.03( ISSN:1567-1356

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/1567-1364.12098

  • Isolation and characterization of baker's yeast capable of strongly activating a macrophage Reviewed

    Yuki Takada, Yumiko Nishino, Chinatsu Ito, Hajime Watanabe, Ken Kanzaki, Taro Tachibana, Masayuki Azuma

    FEMS YEAST RESEARCH   14 ( 2 )   261 - 269   2014.03( ISSN:1567-1356

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    A physiological function of the -glucans which constitute the cell wall of Saccharomyces cerevisiae is to activate immune cells. Here, we focused on the immunostimulation ability of S.cerevisiae itself to give this ability to fermented foods including yeast. Previously, we found that in S.cerevisiae the deletion of MCD4 gene causes exposure of -glucans on the cell surface and that the mcd4 deletion mutant strongly enhances immunity in vitro and in vivo. However, this is not a practical strain but a genetically modified strain with an antibiotic resistance gene, and growth was very slow. The aim of this study was to acquire a practical strain capable of strongly activating a macrophage. The parental strain y-21 was mutated with ethyl methanesulfonate, and the resulting strain was screened. Two mutants (AP-57 and AQ-37) were obtained. AQ-37 had the same fermentation capacity as y-21. In addition, a mutation point of AQ-37 was identified, suggesting that the mutation of NDD1 gene affects the cell wall structure and confers a high ability for macrophage stimulation. The obtained yeast may activate immune cells in materials to which the yeast is added.

    DOI: 10.1111/1567-1364.12098

    PubMed

  • A milliliter-scale yeast-based fuel cell with high performance Reviewed

    Kaneshiro Hiroyuki, Takano Kosuke, Takada Yogo, Wakisaka Tomoyuki, Tachibana Taro, Azuma Masayuki

    BIOCHEMICAL ENGINEERING JOURNAL   83   90 - 96   2014.02( ISSN:1369-703X

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    DOI: 10.1016/j.bej.2013.12.011

  • Generation of a monoclonal antibody for INI1/hSNF5/BAF47 Reviewed

    Akihito Harada, Masayasu Hayashi, Yuuki Kuniyoshi, Yuichiro Semba, Satoko Sugahara, Taro Tachibana, Yasuyuki Ohkawa, Masatoshi Fujita

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   33 ( 1 )   49 - 51   2014.02( ISSN:2167-9436

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    INI1/hSNF5/BAF47, which has an SNF5 domain, belongs to the SWI/SNF family. This family is known as ATP-dependent regulators of gene expression by remodeling chromatin structure during cell differentiation. However, the detailed function of INI1/hSNF5/BAF47 is unclear. Here we report the generation of a specific monoclonal antibody for INI1/hSNF5/BAF47 by the mouse iliac lymph node method. The obtained antibody recognized two isoforms of INI1/hSNF5/BAF47 in immunoblotting and precisely recognized the nuclear localization of INI1/hSNF5/BAF47 in immunostaining. This antibody can contribute to further elucidation of the mechanisms of gene expression regulation by INI1/hSNF5/BAF47 during cell differentiation. © 2014, Mary Ann Liebert, Inc.

    DOI: 10.1089/mab.2013.0065

    PubMed

  • Production of a monoclonal antibody for C/EBPβ: The subnuclear localization of C/EBPβ in mouse L929 cells Reviewed

    Akihito Harada, Etsuko Okazaki, Seiji Okada, Taro Tachibana, Yasuyuki Ohkawa

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   33 ( 1 )   34 - 37   2014.02

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    The CCAAT/enhancer-binding protein (C/EBP)β belongs to the C/EBP family of proteins that possesses a basic leucine zipper DNA-binding domain. These proteins bind DNA by dimerization and play a role in the transcriptional regulation of various cells. There are six different types of C/EBPs, and some form isoforms through the use of alternative translation initiation sites. The functional analysis of the C/EBP family is therefore difficult to achieve. Here we report on the production of specific monoclonal antibodies against mouse C/EBPβ using a rat medial iliac lymph node method. Immunoblotting using C/EBPβ monoclonal antibodies identified two types of isoforms, while immunostaining revealed a subnuclear localization for C/EBPβ. Use of this antibody should contribute to the further elucidation of the transcriptional regulatory function of C/EBPβ. © 2014, Mary Ann Liebert, Inc.

    DOI: 10.1089/mab.2013.0069

    PubMed

  • Cell wall structure suitable for surface display of proteins in Saccharomyces cerevisiae Reviewed

    Matsuoka Hiroyuki, Hashimoto Kazuya, Saijo Aki, Takada Yuki, Kondo Akihiko, Ueda Mitsuyoshi, Ooshima Hiroshi, Tachibana Taro, Azuma Masayuki

    YEAST   31 ( 2 )   67 - 76   2014.02( ISSN:0749-503X

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    DOI: 10.1002/yea.2995

  • Generation of a monoclonal antibody for INI1/hSNF5/BAF47. Reviewed

    Harada A, Hayashi M, Kuniyoshi Y, Semba Y, Sugahara S, Tachibana T, Ohkawa Y, Fujita M

    Monoclonal antibodies in immunodiagnosis and immunotherapy   33 ( 1 )   49 - 51   2014.02

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    DOI: 10.1089/mab.2013.0065

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  • Production of a monoclonal antibody for C/EBPβ: the subnuclear localization of C/EBPβ in mouse L929 cells. Reviewed

    Harada A, Okazaki E, Okada S, Tachibana T, Ohkawa Y

    Monoclonal antibodies in immunodiagnosis and immunotherapy   33 ( 1 )   34 - 7   2014.02

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    DOI: 10.1089/mab.2013.0069

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  • A milliliter-scale yeast-based fuel cell with high performance Reviewed

    Hiroyuki Kaneshiro, Kosuke Takano, Yogo Takada, Tomoyuki Wakisaka, Taro Tachibana, Masayuki Azuma

    BIOCHEMICAL ENGINEERING JOURNAL   83   90 - 96   2014.02( ISSN:1369-703X

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    Microbial fuel cells are attracting attention as one of the systems for producing electrical energy from organic compounds. We used commercial baker's yeast (Saccharomyces cerevisiae) for a glucose fuel cell because the yeast is a safe organism and relatively high power can be generated in the system. In the present study, a milliliter (mL)-scale dual-chamber fuel cell was constructed for evaluating the power generated by a variety of yeasts and their mutants, and the optimum conditions for high performance were investigated. When carbon fiber bundles were used as an electrode in the fuel cell, high volumetric power density was obtained. The maximum power produced per volume of anode solution was 850 W/m(3) under optimum conditions. Furthermore, the power was examined using seven kinds of yeast. In Kluyveromyces marxianus, not only the power but also the power per consumed glucose was high. Moreover, it was suggested that xylose is available as fuel for the fuel cell. The fuel cell powered by K. marxianus may prove to be helpful for the effective utilization of woody biomass. (C) 2014 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.bej.2013.12.011

  • Cell wall structure suitable for surface display of proteins in Saccharomyces cerevisiae Reviewed

    Hiroyuki Matsuoka, Kazuya Hashimoto, Aki Saijo, Yuki Takada, Akihiko Kondo, Mitsuyoshi Ueda, Hiroshi Ooshima, Taro Tachibana, Masayuki Azuma

    YEAST   31 ( 2 )   67 - 76   2014.02( ISSN:0749-503X

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    A display system for adding new protein functions to the cell surfaces of microorganisms has been developed, and applications of the system to various fields have been proposed. With the aim of constructing a cell surface environment suitable for protein display in Saccharomyces cerevisiae, the cell surface structures of cell wall mutants were investigated. Four cell wall mutant strains were selected by analyses using a GFP display system via a GPI anchor. -Glucosidase and endoglucanase II were displayed on the cell surface in the four mutants, and their activities were evaluated. mnn2 deletion strain exhibited the highest activity for both the enzymes. In particular, endoglucanase II activity using carboxymethylcellulose as a substrate in the mutant strain was 1.9-fold higher than that of the wild-type strain. In addition, the activity of endoglucanase II released from the mnn2 deletion strain by Zymolyase 20T treatment was higher than that from the wild-type strain. The results of green fluorescent protein (GFP) and endoglucanase displays suggest that the amounts of enzyme displayed on the cell surface were increased by the mnn2 deletion. The enzyme activity of the mnn2 deletion strain was compared with that of the wild-type strain. The relative value (mnn2 deletion mutant/wild-type strain) of endoglucanase II activity using carboxymethylcellulose as a substrate was higher than that of -glucosidase activity using p-nitrophenyl--glucopyranoside as a substrate, suggesting that the cell surface environment of the mnn2 deletion strain facilitates the binding of high-molecular-weight substrates to the active sites of the displayed enzymes. Copyright (c) 2014 John Wiley & Sons, Ltd.

    DOI: 10.1002/yea.2995

    PubMed

  • Silencing of poly(ADP-ribose) glycohydrolase sensitizes lung cancer cells to radiation through the abrogation of DNA damage checkpoint Reviewed

    Nakadate Yusuke, Kodera Yasuo, Kitamura Yuka, Tachibana Taro, Tamura Tomohide, Koizumi Fumiaki

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   441 ( 4 )   793 - 798   2013.11( ISSN:0006-291X

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    DOI: 10.1016/j.bbrc.2013.10.134

  • Silencing of poly(ADP-ribose) glycohydrolase sensitizes lung cancer cells to radiation through the abrogation of DNA damage checkpoint Reviewed

    Yusuke Nakadate, Yasuo Kodera, Yuka Kitamura, Taro Tachibana, Tomohide Tamura, Fumiaki Koizumi

    Biochemical and Biophysical Research Communications   441 ( 4 )   793 - 798   2013.11( ISSN:0006-291X

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    Poly(ADP-ribose) glycohydrolase (PARG) is a major enzyme that plays a role in the degradation of poly(ADP-ribose) (PAR). PARG deficiency reportedly sensitizes cells to the effects of radiation. In lung cancer, however, it has not been fully elucidated. Here, we investigated whether PARG siRNA contributes to an increased radiosensitivity using 8 lung cancer cell lines. Among them, the silencing of PARG induced a radiosensitizing effect in 5 cell lines. Radiation-induced G2/M arrest was largely suppressed by PARG siRNA in PC-14 and A427 cells, which exhibited significantly enhanced radiosensitivity in response to PARG knockdown. On the other hand, a similar effect was not observed in H520 cells, which did not exhibit a radiosensitizing effect. Consistent with a cell cycle analysis, radiation-induced checkpoint signals were not well activated in the PC-14 and A427 cells when treated with PARG siRNA. These results suggest that the increased sensitivity to radiation induced by PARG knockdown occurs through the abrogation of radiation-induced G2/M arrest and checkpoint activation in lung cancer cells. Our findings indicate that PARG could be a potential target for lung cancer treatments when used in combination with radiotherapy. © 2013 Elsevier Inc.

    DOI: 10.1016/j.bbrc.2013.10.134

    PubMed

  • Functional activity of RLIM/Rnf12 is regulated by phosphorylation-dependent nucleocytoplasmic shuttling. Reviewed

    Jiao B, Taniguchi-Ishigaki N, Güngör C, Peters MA, Chen YW, Riethdorf S, Drung A, Ahronian LG, Shin J, Pagnis R, Pantel K, Tachibana T, Lewis BC, Johnsen SA, Bach I

    Molecular biology of the cell   24 ( 19 )   3085 - 96   2013.10( ISSN:1059-1524

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    DOI: 10.1091/mbc.E13-05-0239

    PubMed

  • Functional activity of RLIM/Rnf12 is regulated by phosphorylation-dependent nucleocytoplasmic shuttling Reviewed

    Baowei Jiao, Naoko Taniguchi-Ishigaki, Cenap Guengoer, Marvin A. Peters, Ya-Wen Chen, Sabine Riethdorf, Alexander Drung, Leanne G. Ahronian, JongDae Shin, Rachna Pagnis, Klaus Pantel, Taro Tachibana, Brian C. Lewis, Steven A. Johnsen, Ingolf Bach

    MOLECULAR BIOLOGY OF THE CELL   24 ( 19 )   3085 - 3096   2013.10( ISSN:1059-1524

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    The X-linked gene Rnf12 encodes the ubiquitin ligase really interesting new gene (RING) finger LIM domain-interacting protein (RLIM)/RING finger protein 12 (Rnf12), which serves as a major sex-specific epigenetic regulator of female mouse nurturing tissues. Early during embryogenesis, RLIM/Rnf12 expressed from the maternal allele is crucial for the development of extraembryonic trophoblast cells. In contrast, in mammary glands of pregnant and lactating adult females RLIM/Rnf12 expressed from the paternal allele functions as a critical survival factor for milk-producing alveolar cells. Although RLIM/Rnf12 is detected mostly in the nucleus, little is known about how and in which cellular compartment(s) RLIM/Rnf12 mediates its biological functions. Here we demonstrate that RLIM/Rnf12 protein shuttles between nucleus and cytoplasm and this is regulated by phosphorylation of serine S214 located within its nuclear localization sequence. We show that shuttling is important for RLIM to exert its biological functions, as alveolar cell survival activity is inhibited in cells expressing shuttling-deficient nuclear or cytoplasmic RLIM/Rnf12. Thus regulated nucleocytoplasmic shuttling of RLIM/Rnf12 coordinates cellular compartments during mammary alveolar cell survival.

    DOI: 10.1091/mbc.E13-05-0239

    PubMed

  • Altered expression of dermokine in skin disorders Reviewed

    Hasegawa M., Higashi K., Yokoyama C., Yamamoto F., Tachibana T., Matsushita T., Hamaguchi Y., Saito K., Fujimoto M., Takehara K.

    JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY   27 ( 7 )   867 - 875   2013.07( ISSN:0926-9959

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    DOI: 10.1111/j.1468-3083.2012.04598.x

  • Altered expression of dermokine in skin disorders Reviewed

    M. Hasegawa, K. Higashi, C. Yokoyama, F. Yamamoto, T. Tachibana, T. Matsushita, Y. Hamaguchi, K. Saito, M. Fujimoto, K. Takehara

    Journal of the European Academy of Dermatology and Venereology   27 ( 7 )   867 - 875   2013.07( ISSN:0926-9959

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    Background Although dermokine-β, a glycoprotein expressed in epithelial cells, does not have significant homology to other proteins, its carboxyl-terminal domain shares a high pI value with many cytokines, suggesting similar functions. Objective To better understand the biology of dermokine, we here determined its localization under pathological conditions and examined factors that regulate its expression. Methods We generated an anti-human dermokine-β/γ monoclonal antibody cross-reacting with the mouse protein. Using this antibody, immunohistological staining and Western blotting of dermokine-β/γ were performed with various tissue samples. Results Although human dermokine-β/γ was expressed in almost all granular layers, upper spinous layers of the skin were also stained with anti-dermokine-β/γ antibody in inflammatory skin disorders. Dermokine-β/γ was expressed in keratoacanthoma and a part of well-differentiated squamous cell carcinoma (SCC). However, dermokine-β/ γ was not detected in poorly differentiated SCC or tumours derived from non-keratinocytes. In mice, dermokine-β/γ-expressed keratinocytes were increased in models of contact hypersensitivity, ultraviolet-irradiated skin injury and wound healing. Consistent with expanded distribution in inflammatory skin diseases, proinflammatory cytokines such as interleukin-1β, interleukin-12, and tumour necrosis factor-α augmented dermokine-β/γ expression in cultured human keratinocytes. In contrast, growth factors including epidermal growth factor, insulin-like growth factor-I, keratinocyte growth factor and transforming growth factor-α significantly reduced dermokine expression. Conclusion These results provide novel insights into the physiological and pathological significance of dermokine in the epidermis. © 2012 European Academy of Dermatology and Venereology.

    DOI: 10.1111/j.1468-3083.2012.04598.x

    PubMed

  • Production of a monoclonal antibody specific for Pou5f1/Oct4 Reviewed

    Tatsuhiko Arakawa, Tomohiko Yoshimi, Masayuki Azuma, Taro Tachibana

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   32 ( 3 )   229 - 231   2013.06( ISSN:2167-9436

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    Pou5f1/Oct4, a member of the POU transcription factor family, is exclusively expressed in embryonic stem cells, which are involved in self-renewal and maintaining pluripotency. In the present study, we report on the establishment of a monoclonal antibody that is specific for Oct4 using the rat medial iliac lymph node method. In an immunoblotting analysis, our antibody detected endogenous Oct4. In addition, immunocytochemical staining using the antibody revealed the nuclear localization of Oct4. This monoclonal antibody has the potential for use in the further analysis of Oct4 function in stem cells. © Copyright 2013, Mary Ann Liebert, Inc. 2013.

    DOI: 10.1089/mab.2013.0004

    PubMed

  • Production of a monoclonal antibody specific for Pou5f1/Oct4. Reviewed

    Arakawa T, Yoshimi T, Azuma M, Tachibana T

    Monoclonal antibodies in immunodiagnosis and immunotherapy   32 ( 3 )   229 - 31   2013.06

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    DOI: 10.1089/mab.2013.0004

    PubMed

  • A panel of specific monoclonal antibodies directed against various phosphorylated histones H3 Reviewed

    Tomohiko Yoshimi, Yasuyuki Ohkawa, Masayuki Azuma, Taro Tachibana

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   32 ( 2 )   119 - 124   2013.04( ISSN:2167-9436

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    Modification of histone plays a critical role in the epigenetic regulation of gene expression. However, unlike the widely studied roles of histone methylation or acetylation of histone H3, relatively little is known about the molecular mechanisms involved in translating histone phosphorylation into a specific outcome. The present study reports on the development of antibodies (MAbs) directed against phosphorylated histone H3 (S10, T11, S28, S31, and T32), produced by the hybridization of mouse myeloma cells with lymph node cells from an immunized rat or mouse. The MAbs produced specifically recognize different sites of phosphorylation on histone H3. All of these MAbs are suitable for immunoblotting and immunofluorescence analysis. We believe that these antibodies should significantly facilitate our efforts to investigate epigenetic regulation. © Mary Ann Liebert, Inc.

    DOI: 10.1089/mab.2012.0105

    PubMed

  • Production of a Monoclonal Antibody Specific for Pou5f1/Oct4.

    Arakawa T, Yoshimi T, Azuma M, Tachibana T.

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy,   2013.04

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  • A panel of specific monoclonal antibodies directed against various phosphorylated histones H3. Reviewed

    Yoshimi T, Ohkawa Y, Azuma M, Tachibana T

    Monoclonal antibodies in immunodiagnosis and immunotherapy   32 ( 2 )   119 - 24   2013.04

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    DOI: 10.1089/mab.2012.0105

    PubMed

  • Production of a Monoclonal Antibody Specific for Pou5f1/Oct4. Reviewed

    Arakawa T, Yoshimi T, Azuma M, Tachibana T.

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy,   2013.04

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  • A panel of specific monoclonal antibodies directed against various phosphorylated histone H3.

    Yoshimi T., Ohkawa Y., Azuma M., Tachibana T.

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy,   2013.03

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  • A panel of specific monoclonal antibodies directed against various phosphorylated histone H3. Reviewed

    Yoshimi T., Ohkawa Y., Azuma M., Tachibana T.

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy,   2013.03

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  • Substrate specificity of Pasteurella multocida toxin for alpha subunits of heterotrimeric G proteins Reviewed

    Orth Joachim H. C., Fester Ines, Siegert Peter, Weise Markus, Lanner Ulrike, Kamitani Shigeki, Tachibana Taro, Wilson Brenda A., Schlosser Andreas, Horiguchi Yasuhiko, Aktories Klaus

    FASEB JOURNAL   27 ( 2 )   832 - 842   2013.02( ISSN:0892-6638

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    DOI: 10.1096/fj.12-213900

  • Substrate specificity of Pasteurella multocida toxin for alpha subunits of heterotrimeric G proteins Reviewed

    Orth Joachim H. C, Fester Ines, Siegert Peter, Weise Markus, Lanner Ulrike, Kamitani Shigeki, Tachibana Taro, Wilson Brenda A, Schlosser Andreas, Horiguchi Yasuhiko, Aktories Klaus

    FASEB JOURNAL   27 ( 2 )   832 - 842   2013.02( ISSN:0892-6638

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    Pasteurella multocida is the causative agent of a number of epizootic and zoonotic diseases. Its major virulence factor associated with atrophic rhinitis in animals and dermonecrosis in bite wounds is P. multocida toxin (PMT). PMT stimulates signal transduction pathways downstream of heterotrimeric G proteins, leading to effects such as mitogenicity, blockade of apoptosis, or inhibition of osteoblast differentiation. On the basis of Gα , it was demonstrated that the toxin deamidates an essential glutamine residue of the Gαi2 subunit, leading to constitutive activation of the G protein. Here, we studied the specificity of PMT for its G-protein targets by mass spectrometric analyses and by utilizing a monoclonal antibody, which recognizes specifically G proteins deamidated by PMT. The studies revealed deamidation of 3 of 4 families of heterotrimeric G proteins (Gα , Gαi and Gα of mouse or human origin) by PMT but not by a catalytic inactive toxin mutant. With the use of G-protein fragments and chimeras of responsive or unresponsive G proteins, the structural basis for the discrimination of heterotrimeric G proteins was studied. Our results elucidate substrate specificity of PMT on the molecular level and provide evidence for the underlying structural reasons of substrate discrimination. © FASEB. i2 q/11 1,2,3, 12/13

    DOI: 10.1096/fj.12-213900

    PubMed

  • Contribution of Leydig and Sertoli cells to testosterone production in mouse fetal testes.

    Shima Y, Miyabayashi K, Haraguchi S, Arakawa T, et al.,

    Mol Endocrinol   27   63 - 73   2013.01

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  • Contribution of Leydig and Sertoli Cells to Testosterone Production in Mouse Fetal Testes Reviewed

    Shima Yuichi, Miyabayashi Kanako, Haraguchi Shogo, Arakawa Tatsuhiko, Otake Hiroyuki, Baba Takashi, Matsuzaki Sawako, Shishido Yurina, Akiyama Haruhiko, Tachibana Taro, Tsutsui Kazuyoshi, Morohashi Ken-ichirou

    MOLECULAR ENDOCRINOLOGY   27 ( 1 )   63 - 73   2013.01( ISSN:0888-8809

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    DOI: 10.1210/me.2012-1256

  • A co-localization model of paired ChIP-seq data using a large ENCODE data set enables comparison of multiple samples Reviewed

    Maehara Kazumitsu, Odawara Jun, Harada Akihito, Yoshimi Tomohiko, Nagao Koji, Obuse Chikashi, Akashi Koichi, Tachibana Taro, Sakata Toshio, Ohkawa Yasuyuki

    NUCLEIC ACIDS RESEARCH   41 ( 1 )   54 - 62   2013.01( ISSN:0305-1048

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    DOI: 10.1093/nar/gks1010

  • A co-localization model of paired ChIP-seq data using a large ENCODE data set enables comparison of multiple samples.

    Maehara K., Odawara J., Harada A., Yoshimi T., Nagao K., Obuse C., Akashi K., Tachibana T., Sakata T., Ohkawa Y.

    Nucleic Acids Res.   41   54 - 62   2013.01

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  • Contribution of Leydig and Sertoli cells to testosterone production in mouse fetal testes. Reviewed

    Shima Y, Miyabayashi K, Haraguchi S, Arakawa T, et al.,

    Mol Endocrinol   27   63 - 73   2013.01

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  • Contribution of Leydig and Sertoli Cells to Testosterone Production in Mouse Fetal Testes Reviewed

    Yuichi Shima, Kanako Miyabayashi, Shogo Haraguchi, Tatsuhiko Arakawa, Hiroyuki Otake, Takashi Baba, Sawako Matsuzaki, Yurina Shishido, Haruhiko Akiyama, Taro Tachibana, Kazuyoshi Tsutsui, Ken-ichirou Morohashi

    MOLECULAR ENDOCRINOLOGY   27 ( 1 )   63 - 73   2013.01( ISSN:0888-8809

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    Testosterone is a final product of androgenic hormone biosynthesis, and Leydig cells are known to be the primary source of androgens. In the mammalian testis, two distinct populations of Leydig cells, the fetal and the adult Leydig cells, develop sequentially, and these two cell types differ both morphologically and functionally. It is well known that the adult Leydig cells maintain male reproductive function by producing testosterone. However, it has been controversial whether fetal Leydig cells can produce testosterone, and the synthetic pathway of testosterone in the fetal testis is not fully understood. In the present study, we generated transgenic mice in which enhanced green fluorescence protein was expressed under the control of a fetal Leydig cell-specific enhancer of the Ad4BP/SF-1 (Nr5a1) gene. The transgene construct was prepared by mutating the LIM homeodomain transcription factor (LHX9)-binding sequence in the promoter, which abolished promoter activity in the undifferentiated testicular cells. These transgenic mice were used to collect highly pure fetal Leydig cells. Gene expression and steroidogenic enzyme activities in the fetal Leydig cells as well as in the fetal Sertoli cells and adult Leydig cells were analyzed. Our results revealed that the fetal Leydig cells synthesize only androstenedione because they lack expression of Hsd17b3, and fetal Sertoli cells convert androstenedione to testosterone, whereas adult Leydig cells synthesize testosterone by themselves. The current study demonstrated that both Leydig and Sertoli cells are required for testosterone synthesis in the mouse fetal testis. (Molecular Endocrinology 27: 63-73, 2013)

    DOI: 10.1210/me.2012-1256

  • A co-localization model of paired ChIP-seq data using a large ENCODE data set enables comparison of multiple samples Reviewed

    Kazumitsu Maehara, Jun Odawara, Akihito Harada, Tomohiko Yoshimi, Koji Nagao, Chikashi Obuse, Koichi Akashi, Taro Tachibana, Toshio Sakata, Yasuyuki Ohkawa

    NUCLEIC ACIDS RESEARCH   41 ( 1 )   54 - 62   2013.01( ISSN:0305-1048

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    Deep sequencing approaches, such as chromatin immunoprecipitation by sequencing (ChIP-seq), have been successful in detecting transcription factor-binding sites and histone modification in the whole genome. An approach for comparing two different ChIP-seq data would be beneficial for predicting unknown functions of a factor. We propose a model to represent co-localization of two different ChIP-seq data. We showed that a meaningful overlapping signal and a meaningless background signal can be separated by this model. We applied this model to compare ChIP-seq data of RNA polymerase II C-terminal domain (CTD) serine 2 phosphorylation with a large amount of peak-called data, including ChIP-seq and other deep sequencing data in the Encyclopedia of DNA Elements (ENCODE) project, and then extracted factors that were related to RNA polymerase II CTD serine 2 in HeLa cells. We further analyzed RNA polymerase II CTD serine 7 phosphorylation, of which their function is still unclear in HeLa cells. Our results were characterized by the similarity of localization for transcription factor/histone modification in the ENCODE data set, and this suggests that our model is appropriate for understanding ChIP-seq data for factors where their function is unknown.

    DOI: 10.1093/nar/gks1010

  • Custom monoclonal antibody production service by University-originated bio-tech venture Reviewed

    Tachibana Taro

    JOURNAL OF PHYSIOLOGICAL SCIENCES   63   S105 - S105   2013( ISSN:1880-6546

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  • Custom monoclonal antibody production service by University-originated bio-tech venture Reviewed

    Tachibana Taro

    JOURNAL OF PHYSIOLOGICAL SCIENCES   63   S105 - S105   2013( ISSN:1880-6546

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  • Custom monoclonal antibody production service by University-originated bio-tech venture Reviewed

    Taro Tachibana

    JOURNAL OF PHYSIOLOGICAL SCIENCES   63   S105 - S105   2013( ISSN:1880-6546

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  • Dermokine-beta impairs ERK signaling through direct binding to GRP78 Reviewed

    Higashi Kiyoshi, Hasegawa Minoru, Yokoyama Chikako, Tachibana Taro, Mitsui Shinichi, Saito Koichi

    FEBS LETTERS   586 ( 16 )   2300 - 2305   2012.07( ISSN:0014-5793

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    DOI: 10.1016/j.febslet.2012.06.022

  • Dermokine-β impairs ERK signaling through direct binding to GRP78.

    FEBS Lett.   586   2300 - 2305   2012.07

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  • Dermokine-β impairs ERK signaling through direct binding to GRP78. Reviewed

    FEBS Lett.   586   2300 - 2305   2012.07

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  • Dermokine-beta impairs ERK signaling through direct binding to GRP78 Reviewed

    Kiyoshi Higashi, Minoru Hasegawa, Chikako Yokoyama, Taro Tachibana, Shinichi Mitsui, Koichi Saito

    FEBS LETTERS   586 ( 16 )   2300 - 2305   2012.07( ISSN:0014-5793

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    Dermokine-beta is abundant in stratified epithelia and in differentiating cultured keratinocytes. In this study, we investigated the role of dermokine-beta in differentiation of keratinocytes. Treatment of keratinocytes or skin tumor cells with dermokine-beta attenuated phosphorylation of extracellular-signal-regulated kinase (ERK). Exposure of cells to dermokine-beta, as well as its carboxyl-terminus domain peptide, interrupted phosphorylation of ERK and stimulated dermokine gene expression. Inhibition of ERK signaling by its specific inhibitor also increased dermokine expression level. A combination of chemical cross-linking and immunoprecipitation, followed by proteomics analyses, identified glucose-regulated protein 78 (GRP78) as a dermokine-beta-associated protein. Blockage of GRP78 expression by a specific siRNA abrogated actions of dermokine-beta. These findings provide novel insights into the physiological significance of dermokine-beta in the epidermis.

    DOI: 10.1016/j.febslet.2012.06.022

  • Chd2 interacts with H3.3 to determine myogenic cell fate Reviewed

    Harada Akihito, Okada Seiji, Konno Daijiro, Odawara Jun, Yoshimi Tomohiko, Yoshimura Saori, Kumamaru Hiromi, Saiwai Hirokazu, Tsubota Toshiaki, Kurumizaka Hitoshi, Akashi Koichi, Tachibana Taro, Imbalzano Anthony N., Ohkawa Yasuyuki

    EMBO JOURNAL   31 ( 13 )   2994 - 3007   2012.06( ISSN:0261-4189

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    DOI: 10.1038/emboj.2012.136

  • Chd2 interacts with H3.3 to determine myogenic cell fate Reviewed

    Akihito Harada, Seiji Okada, Daijiro Konno, Jun Odawara, Tomohiko Yoshimi, Saori Yoshimura, Hiromi Kumamaru, Hirokazu Saiwai, Toshiaki Tsubota, Hitoshi Kurumizaka, Koichi Akashi, Taro Tachibana, Anthony N. Imbalzano, Yasuyuki Ohkawa

    EMBO JOURNAL   31 ( 13 )   2994 - 3007   2012.06( ISSN:0261-4189

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    Cell differentiation is mediated by lineage-determining transcription factors. We show that chromodomain helicase DNA-binding domain 2 (Chd2), a SNF2 chromatin remodelling enzyme family member, interacts with MyoD and myogenic gene regulatory sequences to specifically mark these loci via deposition of the histone variant H3.3 prior to cell differentiation. Directed and genome-wide analysis of endogenous H3.3 incorporation demonstrates that knockdown of Chd2 prevents H3.3 deposition at differentiation-dependent, but not housekeeping, genes and inhibits myogenic gene activation. The data indicate that MyoD determines cell fate and facilitates differentiation-dependent gene expression through Chd2-dependent deposition of H3.3 at myogenic loci prior to differentiation. The EMBO Journal (2012) 31, 2994-3007. doi: 10.1038/emboj.2012.136; Published online 8 May 2012

    DOI: 10.1038/emboj.2012.136

  • Chd2 interacts with H3.3 to determine myogenic cell fate.

    Harada A, Okada S, Konno D, Odawara J, Yoshimi T, Yoshimura S, et al.,

    EMBO J.   31   2994 - 3007   2012.05

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  • Altered expression of dermokine in skin disorders.

    Hasegawa M, Higashi K, Yokoyama C, Yamamoto F, Tachibana T, Matsushita T, Hamaguchi Y, Saito K, Fujimoto M, Takehara K.

    J. Eur. Acad. Dermatol. Venereol.   doi: 10.1111/j.1468-3083.2012.04598.x.   2012.05

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  • Altered expression of dermokine in skin disorders. Reviewed

    Hasegawa M, Higashi K, Yokoyama C, Yamamoto F, Tachibana T, Matsushita T, Hamaguchi Y, Saito K, Fujimoto M, Takehara K.

    J. Eur. Acad. Dermatol. Venereol.   doi: 10.1111/j.1468-3083.2012.   2012.05

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  • Chd2 interacts with H3.3 to determine myogenic cell fate. Reviewed

    Harada A, Okada S, Konno D, Odawara J, Yoshimi T, Yoshimura S, et al.,

    EMBO J.   31   2994 - 3007   2012.05

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  • The cytoplasmic tail of heparin-binding EGF-like growth factor regulates bidirectional intracellular trafficking between the plasma membrane and ER Reviewed

    Hieda Miki, Koizumi Michiko, Higashi Chiduru, Tachibana Taro, Taguchi Tomohiko, Higashiyama Shigeki

    FEBS OPEN BIO   2   339 - 344   2012( ISSN:2211-5463

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    DOI: 10.1016/j.fob.2012.09.002

  • The cytoplasmic tail of heparin-binding EGF-like growth factor regulates bidirectional intracellular trafficking between the plasma membrane and ER Reviewed

    Miki Hieda, Michiko Koizumi, Chiduru Higashi, Taro Tachibana, Tomohiko Taguchi, Shigeki Higashiyama

    FEBS OPEN BIO   2   339 - 344   2012( ISSN:2211-5463

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    Heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF) is synthesized in the ER, transported along the exocytic pathway, and expressed on the plasma membrane as a type I transmembrane protein. Upon extracellular stimulation, HB-EGF, either proHB-EGF or the shed form HB-EGF-CTF, undergoes endocytosis and is then transported retrogradely to the ER. In this study, we showed the essential contribution of the short cytoplasmic tail of HB-EGF (HB-EGF-cyto) to the bidirectional intracellular trafficking between the ER and plasma membrane and revealed several critical amino acids residues that are responsible for internalization from the plasma membrane and ER targeting. We suggest that these anterograde and retrograde sorting signals within HB-EGF-cyto are strictly regulated by protein modification and conformation. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

    DOI: 10.1016/j.fob.2012.09.002

  • Steroidogenic Factor 1 (NR5A1) resides in centrosomes and maintains genomic stability by controlling centrosome homeostasis.

    Lai PY, Wang CY, Chen WY, Kao YH, Tsai HM, Tachibana T, Chang WC, Chung BC.

    Cell Death Differ.   18 ( 12 )   1836 - 1844   2011.12

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  • Steroidogenic Factor 1 (NR5A1) resides in centrosomes and maintains genomic stability by controlling centrosome homeostasis Reviewed

    Lai P-Y, Wang C-Y, Chen W-Y, Kao Y-H, Tsai H-M, Tachibana T., Chang W-C, Chung B-C

    CELL DEATH AND DIFFERENTIATION   18 ( 12 )   1836 - 1844   2011.12( ISSN:1350-9047

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    DOI: 10.1038/cdd.2011.54

  • Steroidogenic Factor 1 (NR5A1) resides in centrosomes and maintains genomic stability by controlling centrosome homeostasis. Reviewed

    Lai PY, Wang CY, Chen WY, Kao YH, Tsai HM, Tachibana T, Chang WC, Chung BC.

    Cell Death Differ.   18 ( 12 )   1836 - 1844   2011.12

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  • Steroidogenic Factor 1 (NR5A1) resides in centrosomes and maintains genomic stability by controlling centrosome homeostasis Reviewed

    P-Y Lai, C-Y Wang, W-Y Chen, Y-H Kao, H-M Tsai, T. Tachibana, W-C Chang, B-C Chung

    CELL DEATH AND DIFFERENTIATION   18 ( 12 )   1836 - 1844   2011.12( ISSN:1350-9047

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    SF-1 (Steroidogenic Factor 1, NR5A1) is a tissue-specific transcription factor critical for the growth, development and differentiation of steroidogenic and a few other endocrine tissues. But how SF-1 regulates cell growth is not entirely clear. Here we found that SF-1 was localized to the centrosome in addition to the nucleus, and SF-1 depletion by shRNA caused centrosome over-duplication, aberrant mitosis and genomic instability, leading to a reduction of cell number. Centrosome amplification defect was rescued by both wild-type SF-1 and transcription-defective SF-1-G35E, suggesting a non-genomic activity of SF-1 involved in centrosome homeostasis. In addition, we identified in SF-1 a centrosome localization signal, whose overexpression led to reduced localization of both SF-1 and gamma-tubulin to the centrosome. Our results uncover a novel role of SF-1 in the control of centrosome homeostasis and genomic stability. Cell Death and Differentiation (2011) 18, 1836-1844; doi:10.1038/cdd.2011.54; published online 13 May 2011

    DOI: 10.1038/cdd.2011.54

  • The classification of mRNA expression levels by the phosphorylation state of RNAPII CTD based on a combined genome-wide approach Reviewed

    Odawara Jun, Harada Akihito, Yoshimi Tomohiko, Maehara Kazumitsu, Tachibana Taro, Okada Seiji, Akashi Koichi, Ohkawa Yasuyuki

    BMC GENOMICS   12   2011.10( ISSN:1471-2164

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    DOI: 10.1186/1471-2164-12-516

  • Cross-talk between distinct nuclear import pathways enables efficient nuclear import of E47 in conjunction with its partner transcription factors Reviewed

    Mehmood Rashid, Yasuhara Noriko, Fukumoto Masahiro, Oe Souichi, Tachibana Taro, Yoneda Yoshihiro

    MOLECULAR BIOLOGY OF THE CELL   22 ( 19 )   3715 - 3724   2011.10( ISSN:1059-1524

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    DOI: 10.1091/mbc.E10-10-0809

  • Cross-talk between distinct nuclear import pathways enables efficient nuclear import of E47 in conjunction with its partner transcription factors Reviewed

    Rashid Mehmood, Noriko Yasuhara, Masahiro Fukumoto, Souichi Oe, Taro Tachibana, Yoshihiro Yoneda

    MOLECULAR BIOLOGY OF THE CELL   22 ( 19 )   3715 - 3724   2011.10( ISSN:1059-1524

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    Nuclear import of karyophilic proteins is carried out by a variety of mechanisms. We previously showed that two basic helix-loop-helix proteins, NeuroD1 and E47, synergistically affect each other&apos;s nuclear import. In this study, we dissected the molecular pathways underlying nuclear import of the NeuroD1/E47 heterodimer. In vitro nuclear import assays indicated that importin alpha family members are the major nuclear import receptors for E47. However, inhibition of importin alpha resulted in cytoplasmic retention of E47 that could be rescued by its binding partner, NeuroD1, through heterodimerization. In addition, nuclear import of NeuroD1 was importin alpha independent but importin beta 1 dependent. In primary neurons, localization of endogenous E47 was not affected by importin alpha inhibition, suggesting that neuronal E47 could be imported into the nucleus as a heterodimer with NeuroD1 by using importin beta 1 alone. We also found that E47 had similar nuclear import characteristics in C2C12 cells, where E47 heterodimerized with MyoD, another helix-loop-helix protein, suggesting functional conservation within the same family of transcription factors. Collectively, our data reveal that E47 is imported into the nucleus via multiple pathways, depending on the molecular binding mode, establishing a previously uncharacterized cross-talk between two distinct nuclear import pathways.

    DOI: 10.1091/mbc.E10-10-0809

  • The classification of mRNA expression levels by the phosphorylation state of RNAPII CTD based on a combined genome-wide approach Reviewed

    Jun Odawara, Akihito Harada, Tomohiko Yoshimi, Kazumitsu Maehara, Taro Tachibana, Seiji Okada, Koichi Akashi, Yasuyuki Ohkawa

    BMC GENOMICS   12   516   2011.10( ISSN:1471-2164

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    Background: Cellular function is regulated by the balance of stringently regulated amounts of mRNA. Previous reports revealed that RNA polymerase II (RNAPII), which transcribes mRNA, can be classified into the pausing state and the active transcription state according to the phosphorylation state of RPB1, the catalytic subunit of RNAPII. However, genome-wide association between mRNA expression level and the phosphorylation state of RNAPII is unclear. While the functional importance of pausing genes is clear, such as in mouse Embryonic Stem cells for differentiation, understanding this association is critical for distinguishing pausing genes from active transcribing genes in expression profiling data, such as microarrays and RNAseq. Therefore, we examined the correlation between the phosphorylation of RNAPII and mRNA expression levels using a combined analysis by ChIPseq and RNAseq.
    Results: We first performed a precise quantitative measurement of mRNA by performing an optimized calculation in RNAseq. We then visualized the recruitment of various phosphorylated RNAPIIs, such as Ser2P and Ser5P. A combined analysis using optimized RNAseq and ChIPseq for phosphorylated RNAPII revealed that mRNA levels correlate with the various phosphorylation states of RNAPII.
    Conclusions: We demonstrated that the amount of mRNA is precisely reflected by the phased phosphorylation of Ser2 and Ser5. In particular, even the most "pausing" genes, for which only Ser5 is phosphorylated, were detectable at a certain level of mRNA. Our analysis indicated that the complexity of quantitative regulation of mRNA levels could be classified into three categories according to the phosphorylation state of RNAPII.

    DOI: 10.1186/1471-2164-12-516

  • Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibodies recognizing the deamidated alpha subunit of the heterotrimeric GTPase G(q) Reviewed

    Kamitani Shigeki, Ao Shinpei, Toshima Hirono, Tachibana Taro, Hashimoto Makiko, Kitadokoro Kengo, Fukui-Miyazaki Aya, Abe Hiroyuki, Horiguchi Yasuhiko

    FEBS JOURNAL   278 ( 15 )   2702 - 2712   2011.08( ISSN:1742-464X

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    DOI: 10.1111/j.1742-4658.2011.08197.x

  • Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibodies recognizing the deamidated alpha subunit of the heterotrimeric GTPase G(q) Reviewed

    Shigeki Kamitani, Shinpei Ao, Hirono Toshima, Taro Tachibana, Makiko Hashimoto, Kengo Kitadokoro, Aya Fukui-Miyazaki, Hiroyuki Abe, Yasuhiko Horiguchi

    FEBS JOURNAL   278 ( 15 )   2702 - 2712   2011.08( ISSN:1742-464X

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    Pasteurella multocida toxin (PMT) is a virulence factor responsible for the pathogenesis of some Pasteurellosis. PMT exerts its toxic effects through the activation of heterotrimeric GTPase (G(q), G(12/13) and G(i))-dependent pathways, by deamidating a glutamine residue in the alpha subunit of these GTPases. However, the enzymatic characteristics of PMT are yet to be analyzed in detail because the deamidation has only been observed in cell-based assays. In the present study, we developed rat monoclonal antibodies, specifically recognizing the deamidated G alpha(q), to detect the actions of PMT by immunological techniques such as western blotting. Using the monoclonal antibodies, we found that the toxin deamidated G alpha(q) only under reducing conditions. The C-terminal region of PMT, C-PMT, was more active than the full-length PMT. The C3 domain possessing the enzyme core catalyzed the deamidation in vitro without any other domains. These results not only support previous observations on toxicity, but also provide insights into the enzymatic nature of PMT. In addition, we present several lines of evidence that G alpha(11), as well as G alpha(q), could be a substrate for PMT.

    DOI: 10.1111/j.1742-4658.2011.08197.x

  • Mitochondrial Prohibitins and Septin 9 Are Implicated in the Onset of Rat Hepatocarcinogenesis Reviewed

    Kakehashi Anna, Ishii Naomi, Shibata Takeshi, Wei Min, Okazaki Etsuko, Tachibana Taro, Fukushima Shoji, Wanibuchi Hideki

    TOXICOLOGICAL SCIENCES   119 ( 1 )   61 - 72   2011.01( ISSN:1096-6080

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    DOI: 10.1093/toxsci/kfq307

  • Mitochondrial Prohibitins and Septin 9 Are Implicated in the Onset of Rat Hepatocarcinogenesis Reviewed

    Anna Kakehashi, Naomi Ishii, Takeshi Shibata, Min Wei, Etsuko Okazaki, Taro Tachibana, Shoji Fukushima, Hideki Wanibuchi

    TOXICOLOGICAL SCIENCES   119 ( 1 )   61 - 72   2011.01( ISSN:1096-6080

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    In the present study, protein lysates from microdissected glutathione S-transferase placental-form-positive (GST-P(+)) foci and hepatocellular carcinomas from livers of rats treated with N-diethylnitrosamine followed by phenobarbital at doses of 0 and 500 ppm in the diet for 10 and 33 weeks were analyzed using QSTAR Elite liquid chromatography with tandem mass spectrometry and iTRAQ technology. Among 75 proteins, a total of 27 and 50 proteins displaying significant quantitative changes comparing with adjacent normal-appearing liver tissue were identified in GST-P(+) foci of initiation control and promotion groups, respectively, which are related to transcription, protein folding, cytoskeleton filaments reorganization, cell cycle control, nuclear factor (erythroid-derived 2)-like 2 (NRF2)-mediated oxidative stress responses, lipid metabolism, glutathione metabolism, oxidative phosphorylation, and signal transduction. Furthermore, Ingenuity Pathway and bioinformatic analyses revealed that expression changes of genes encoding proteins with altered expression detected in GST-P(+) foci are likely to be controlled by c-myc, NRF2, aryl hydrocarbon receptor, nuclear factor kappa B, and hepatocyte nuclear factor 4 transcriptional factors. Coordinated overexpression of mitochondrial chaperons prohibitin (PHB) and prohibitin 2 (PHB2), septin 9 (SEPT9), neurabin 1, and other cytoskeletal and functional proteins in areas of GST-P(+) foci during initiation and/or promotion stages of rat hepatocarcinogenesis was associated with induction of cell proliferation and might be responsible for the neoplastic transformation of rat liver preneoplastic lesions. Newly discovered elevation of PHB, PHB2, and SEPT9 in GST-P(+) foci and tumors, imply that they might play important role in the onset of liver cancer and be of potential values in the studies of hepatocarcinogenesis.

    DOI: 10.1093/toxsci/kfq307

    PubMed

  • Mitochondrial prohibitins and septin 9 are implicated in the onset of rat hepatocarcinogenesis.

    Kakehashi A, Ishii N, Shibata T, Wei M, Okazaki E, Tachibana T, Fukushima S, Wanibuchi H.

    Toxicol Sci.   119   61 - 72   2011

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  • Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibody recognizing the deamidated alpha subunit of the heterotrimeric GTPase Gq.

    Kamitani,S., Ao, S., Toshima, H., Tachibana, T., et al.,

    FEBS Journal   278   2702 - 2712   2011

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  • Cross talk between distinct nuclear import pathways enables efficient nuclear import of E47 in conjunction with its partner transcription factors.

    Mehmood, R., Yasuhara, N., Fukumoto, M., Oe, S., Tachibana, T. and Yoneda, Y.

    Mol.Biol.Cell   22   3715 - 3724   2011

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  • The classification of mRNA expression levels by the phosphorylation state of RNAPII CTD based on a combined genome-wide approach.

    Odawara J, Harada A, Yoshimi T, Maehara K, Tachibana T, Okada S, Akashi K, Ohkawa Y.

    BMC Genomics   12   516   2011

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  • Cross talk between distinct nuclear import pathways enables efficient nuclear import of E47 in conjunction with its partner transcription factors. Reviewed

    Mehmood, R., Yasuhara, N., Fukumoto, M., Oe, S., Tachibana, T. and Yoneda, Y.

    Mol.Biol.Cell   22   3715 - 3724   2011

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  • The classification of mRNA expression levels by the phosphorylation state of RNAPII CTD based on a combined genome-wide approach. Reviewed

    Odawara J, Harada A, Yoshimi T, Maehara K, Tachibana T, Okada S, Akashi K, Ohkawa Y.

    BMC Genomics   12   516   2011

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  • Mitochondrial prohibitins and septin 9 are implicated in the onset of rat hepatocarcinogenesis. Reviewed

    Kakehashi A, Ishii N, Shibata T, Wei M, Okazaki E, Tachibana T, Fukushima S, Wanibuchi H.

    Toxicol Sci.   119   61 - 72   2011

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  • Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibody recognizing the deamidated alpha subunit of the heterotrimeric GTPase Gq. Reviewed

    Kamitani,S., Ao, S., Toshima, H., Tachibana, T., et al.,

    FEBS Journal   278   2702 - 2712   2011

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  • Specific Monoclonal Antibody Against the Nuclear Pore Complex Protein, Nup96 Reviewed

    Mizuguchi Chiaki, Oka Masahiro, Moriyama Tetsuji, Tachibana Taro, Yoneda Yoshihiro

    HYBRIDOMA   29 ( 6 )   551 - 553   2010.12( ISSN:1554-0014

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    DOI: 10.1089/hyb.2010.0065

  • Specific Monoclonal Antibody Against the Nuclear Pore Complex Protein, Nup96 Reviewed

    Chiaki Mizuguchi, Masahiro Oka, Tetsuji Moriyama, Taro Tachibana, Yoshihiro Yoneda

    HYBRIDOMA   29 ( 6 )   551 - 553   2010.12( ISSN:1554-0014

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    Nup96 is a component of the Nup107-160 complex, the largest subunit of the nuclear pore complex. Nup96 is generated as a precursor protein with Nup98. However, the mechanism by which Nup96 contributes to cell function is not clear. We report here on the preparation of a monoclonal antibody (MAb) directed against mouse Nup96. The antibody was produced by the hybridization of mouse myeloma cells with lymph node cells from an immunized rat. The antibody, MAb 4H5, specifically recognized Nup96, as evidenced by immunoblotting using the whole cell lysates. In immunostaining using MAb 4H5, a nuclear rim staining pattern was observed. This antibody will be useful in immunoblotting and immunolocalization experiments, as well as further analyses of the biological function and cellular dynamics of this protein.

    DOI: 10.1089/hyb.2010.0065

  • The Mobile FG Nucleoporin Nup98 Is a Cofactor for Crm1-dependent Protein Export Reviewed

    Oka Masahiro, Asally Munehiro, Yasuda Yoshinari, Ogawa Yutaka, Tachibana Taro, Yoneda Yoshihiro

    MOLECULAR BIOLOGY OF THE CELL   21 ( 11 )   1885 - 1896   2010.06( ISSN:1059-1524

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    DOI: 10.1091/mbc.E09-12-1041

  • The mobile FG nucleoporin Nup98 is a cofactor for Crm1-dependent protein export. Reviewed

    Oka M, Asally M, Yasuda Y, Ogawa Y, Tachibana T, Yoneda Y

    Molecular biology of the cell   21 ( 11 )   1885 - 96   2010.06( ISSN:1059-1524

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    DOI: 10.1091/mbc.e09-12-1041

    PubMed

  • Monoclonal Antibody Specific for Dhx9/NDHII/RHA Reviewed

    Kotani Manato, Harada Akihito, Odawara Jun, Azuma Masayuki, Okada Seiji, Nishiyama Yuko, Nakamura Mako, Tachibana Taro, Ohkawa Yasuyuki

    HYBRIDOMA   29 ( 3 )   259 - 261   2010.06( ISSN:1554-0014

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    DOI: 10.1089/hyb.2009.0107

  • Rat Monoclonal Antibody Specific for MyoD Reviewed

    Harada Akihito, Ohkawa Yasuyuki, Ao Shinpei, Odawara Jun, Okada Seiji, Azuma Masayuki, Nishiyama Yuko, Nakamura Mako, Tachibana Taro

    HYBRIDOMA   29 ( 3 )   255 - 258   2010.06( ISSN:1554-0014

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/hyb.2009.0117

  • Live imaging system for visualizing nuclear pore complex (NPC) formation during interphase in mammalian cells Reviewed

    Iino Haruki, Maeshima Kazuhiro, Nakatomi Reiko, Kose Shingo, Hashikawa Tsutomu, Tachibana Taro, Imamoto Naoko

    GENES TO CELLS   15 ( 6 )   647 - 660   2010.06( ISSN:1356-9597

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    DOI: 10.1111/j.1365-2443.2010.01406.x

  • Rat Monoclonal Antibody Specific for the Chromatin Remodeling Factor, CHD1 Reviewed

    Yoshimura Saori, Harada Akihito, Odawara Jun, Azuma Masayuki, Okada Seiji, Nakamura Mako, Ohkawa Yasuyuki, Tachibana Taro

    HYBRIDOMA   29 ( 3 )   237 - 240   2010.06( ISSN:1554-0014

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    DOI: 10.1089/hyb.2009.0106

  • Live imaging system for visualizing nuclear pore complex (NPC) formation during interphase in mammalian cells Reviewed

    Haruki Iino, Kazuhiro Maeshima, Reiko Nakatomi, Shingo Kose, Tsutomu Hashikawa, Taro Tachibana, Naoko Imamoto

    GENES TO CELLS   15 ( 6 )   647 - 660   2010.06( ISSN:1356-9597

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    Nuclear pore complexes (NPCs) are &apos;supramolecular complexes&apos; on the nuclear envelope assembled from multiple copies of approximately 30 different proteins called nucleoporins (Nups) that provide aqueous channels for nucleocytoplasmic transport during interphase. Although the structural aspects of NPCs have been characterized in detail, NPC formation and its regulation, especially during interphase, are poorly understood. In this study, using the temperature-sensitive RCC1 mutant tsBN2, a baby hamster kidney 21 cell line, we found that a lack of RCC1 activity inhibited NPC formation during interphase, suggesting that RanGTP is required for NPC formation during interphase in mammalian cells. Utilizing the reversible RCC1 activity in tsBN2 cells, we established a live-cell system that allows for the inhibition or initiation of NPC formation by changes in temperature. Our system enables the examination of NPC formation during interphase in living cells. As a lack of RCC1 decreased some Nups containing unstructured phenylalanine-glycine repeats in the NPC structure, we propose that RCC1 is also involved in maintaining NPC integrity during interphase in mammalian cells.

    DOI: 10.1111/j.1365-2443.2010.01406.x

  • Rat Monoclonal Antibody Specific for the Chromatin Remodeling Factor, CHD1 Reviewed

    Saori Yoshimura, Akihito Harada, Jun Odawara, Masayuki Azuma, Seiji Okada, Mako Nakamura, Yasuyuki Ohkawa, Taro Tachibana

    HYBRIDOMA   29 ( 3 )   237 - 240   2010.06( ISSN:1554-0014

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    CHD1 is a subfamily member of the CHD family, which possesses a chromodomain, a helicase domain, and a DNA-binding domain. The CHD family regulates gene expression by contributing to ATP-dependent chromatin remodeling. CHD1 exists in the transcriptionally active region and alters the chromatin structure. Little is known about the function of endogenous CHD1, however, and studies have been hindered by the lack of an antibody specific for CHD1 in mammals. In the present study, we established a monoclonal antibody specifically against CHD1 using the rat medial iliac lymph node method. Immunoblot analysis using our monoclonal antibody showed specific binding to CHD1, allowing us to identify the deduced full-length CHD1. In addition, cell immunostaining clearly revealed the nuclear localization of CHD1. This monoclonal antibody will be useful for further analysis of CHD1 function in mammals.

    DOI: 10.1089/hyb.2009.0106

    PubMed

  • Rat Monoclonal Antibody Specific for MyoD Reviewed

    Akihito Harada, Yasuyuki Ohkawa, Shinpei Ao, Jun Odawara, Seiji Okada, Masayuki Azuma, Yuko Nishiyama, Mako Nakamura, Taro Tachibana

    HYBRIDOMA   29 ( 3 )   255 - 258   2010.06( ISSN:1554-0014

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    Myogenic determination 1 (MyoD) is a myogenic regulatory factor (MRF) possessing a basic domain and a helix-loop-helix domain. MRFs play a critical role in myoblast fate and terminal differentiation. MyoD is a transcriptional factor that induces transcription by binding with gene regulatory factors expressed in skeletal muscle. As a master gene, MyoD also determines skeletal muscle differentiation. In this study, we established a monoclonal antibody specific for MyoD using the rat medial iliac lymph node method. Immunoblot analysis revealed that our monoclonal antibody against MyoD could identify full-length MyoD. Moreover, immunocytochemical staining revealed a change in the expression of MyoD at the skeletal muscle differentiation stage. This monoclonal antibody against MyoD allows for further studies to elucidate the mechanism by which MyoD influences skeletal muscle differentiation.

    DOI: 10.1089/hyb.2009.0117

  • Monoclonal Antibody Specific for Dhx9/NDHII/RHA Reviewed

    Manato Kotani, Akihito Harada, Jun Odawara, Masayuki Azuma, Seiji Okada, Yuko Nishiyama, Mako Nakamura, Taro Tachibana, Yasuyuki Ohkawa

    HYBRIDOMA   29 ( 3 )   259 - 261   2010.06( ISSN:1554-0014

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    Dhx9/NDHII/RHA is a member of the DEAH family of proteins, which possess a double-stranded RNA-binding domain (dsRBD) and a helicase domain. The DEAH protein family plays a critical role in RNA metabolism. DEAH family members function as ATP-dependent RNA helicases and regulation of transcription. In the present study, we report the establishment of a monoclonal antibody specific for Dhx9 using the rat medial iliac lymph node method. Immunoblot analysis using our antibody against Dhx9 detected full-length Dhx9. In addition, immunocytochemical staining using our antibody against Dhx9 revealed the nuclear localization of Dhx9. This monoclonal antibody against Dhx9 will allow for further detailed studies of Dhx9 expression.

    DOI: 10.1089/hyb.2009.0107

    PubMed

  • Rat Monoclonal Antibody Specific for Septin 9 Reviewed

    Tachibana Taro, Okazaki Etsuko, Yoshimi Tomohiko, Azuma Masayuki, Kakehashi Anna, Wanibuchi Hideki

    HYBRIDOMA   29 ( 2 )   169 - 171   2010.04( ISSN:1554-0014

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    DOI: 10.1089/hyb.2009.0092

  • Generation of a Rat Monoclonal Antibody Specific for Chd2 Reviewed

    Harada Akihito, Yoshimura Saori, Odawara Jun, Azuma Masayuki, Okada Seiji, Nakamura Mako, Tachibana Taro, Ohkawa Yasuyuki

    HYBRIDOMA   29 ( 2 )   173 - 177   2010.04( ISSN:1554-0014

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    DOI: 10.1089/hyb.2009.0090

  • Generation of a Rat Monoclonal Antibody Specific for Chd2 Reviewed

    Akihito Harada, Saori Yoshimura, Jun Odawara, Masayuki Azuma, Seiji Okada, Mako Nakamura, Taro Tachibana, Yasuyuki Ohkawa

    HYBRIDOMA   29 ( 2 )   173 - 177   2010.04( ISSN:1554-0014

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    CHD2 is a member of the CHD family that contains chromodomain, helicase domain as well as DNA-binding domain. The CHD family is involved in gene expression and transcription by ATP-dependent chromatin remodeling. Analysis of mutant mouse revealed that CHD2 is involved in development as well as hematopoiesis, which suggests the involvement of CHD2 in gene expression. However, CHD2 has not yet been analyzed biochemically as there is no specific antibody against it. Here, we report on the establishment of specific monoclonal antibody (MAb) against CHD2 utilizing a rat medial iliac lymph node method. Through cell immunostaining utilizing established MAb to CHD2, we confirmed that CHD2 was localized in euchromatin. Additionally, IP-Western revealed that the expression level of full-length CHD2 did not change during the differentiation stage. Additionally, a specific signal was confirmed around 95 kDa at the undifferentiated stage. This clearly indicated that CHD2 was involved in specific gene expression at this stage. Thus, this antibody can contribute to elucidating the function of CHD2 in cell expression.

    DOI: 10.1089/hyb.2009.0090

  • Rat Monoclonal Antibody Specific for Septin 9 Reviewed

    Taro Tachibana, Etsuko Okazaki, Tomohiko Yoshimi, Masayuki Azuma, Anna Kakehashi, Hideki Wanibuchi

    HYBRIDOMA   29 ( 2 )   169 - 171   2010.04( ISSN:1554-0014

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    The septin family of GTPase proteins has been shown to be important for cell division, cytoskeletal organization, and membrane-remodeling events. Septin 9 (SEPT9) is a member of the septin family (also designated MSF/eseptin/Sint1) and has been implicated in tumorigenesis. The present study reports on the preparation and properties of a monoclonal antibody (MAb) directed against SEPT9. The antibody was produced by hybridization of mouse myeloma cells with lymph node cells from an immunized rat. The MAb 7B5 specifically recognized SEPT9, as evidenced by immunoblotting using a variety of extracts from cultured cells. In immunostaining using MAb 7B5, a filamentous pattern near the plasma membrane was observed. The MAb 7B5 promises to be useful in immunoblotting and immunostaining experiments in various cells and tissues to determine the expression levels of SEPT9, as well as to further the analysis of the biological function of this protein.

    DOI: 10.1089/hyb.2009.0092

    PubMed

  • A Rat Monoclonal Antibody Against the Chromatin Remodeling Factor CHD5 Reviewed

    Yoshimura Saori, Yoshimi Tomohiko, Ohkawa Yasuyuki, Azuma Masayuki, Tachibana Taro

    HYBRIDOMA   29 ( 1 )   63 - 66   2010.02( ISSN:1554-0014

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    DOI: 10.1089/hyb.2009.0069

  • Production and Characterization of Monoclonal Antibodies to Mouse Germ Cells Reviewed

    yokoyama Chikako, Katoh-Fukui Yuko, Morohashi Ken-ichirou, Konno Daijiro, Azuma Masayuki, Tachibana Taro

    HYBRIDOMA   29 ( 1 )   53 - 57   2010.02( ISSN:1554-0014

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    DOI: 10.1089/hyb.2009.0101

  • Monoclonal Antibody 3G4 Anti-Brg1 Reviewed

    Tachibana Taro

    HYBRIDOMA   29 ( 1 )   81 - 81   2010.02( ISSN:1554-0014

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    DOI: 10.1089/hyb.2009.0078.MAb

  • A Rat Monoclonal Antibody Against the Chromatin Remodeling Factor CHD5 Reviewed

    Saori Yoshimura, Tomohiko Yoshimi, Yasuyuki Ohkawa, Masayuki Azuma, Taro Tachibana

    HYBRIDOMA   29 ( 1 )   63 - 66   2010.02( ISSN:1554-0014

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    CHD5 (chromodomain/helicase/DNA-binding protein 5) is a member of the CHD subfamily of chromatin remodeling Swi/Snf proteins, and has been recently identified as a tumor suppressor in a diverse range of human cancers. We report here on the establishment of a hybridoma cell line for producing a monoclonal antibody against CHD5 by the rat medial iliac lymph node method. Immunoblotting analyses indicated that this antibody, MAb 5A10, specifically recognizes endogenous CHD5. In immunostaining using the antibody, a nuclear staining pattern was observed. The monoclonal antibody will be useful in immunoblotting and immunolocalization experiments in a variety of cells and tissues, as well as in further studies of the biological function and cellular dynamics of this protein.

    DOI: 10.1089/hyb.2009.0069

    PubMed

  • Production and Characterization of Monoclonal Antibodies to Mouse Germ Cells Reviewed

    Chikako yokoyama, Yuko Katoh-Fukui, Ken-ichirou Morohashi, Daijiro Konno, Masayuki Azuma, Taro Tachibana

    HYBRIDOMA   29 ( 1 )   53 - 57   2010.02( ISSN:1554-0014

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    In mammals, primordial germ cells (PGCs) are generated in the extra-embryonic epiblast, and thereafter migrate into the developing gonads. Following the development of the gonads to the testes or ovaries, germ cells mature into sperms or eggs. In the present study, we report production and characterization of monoclonal antibodies (MAb) that recognize PGCs. Extracts from E12.5 mouse embryonic gonads were immunized as an antigen, and hybridomas were generated using the rat medial iliac lymph node method. The hybridoma supernatants were screened by immunohistochemical analyses of E12.5 mouse embryonic sections. The antibody, referred to herein as MAb 5B5, provided strong signals on PGCs. Moreover, immunofluorescence analyses using a variety of the tissue sections of mouse embryos revealed that MAb 5B5 also recognizes the ventricular zone of the cerebral cortex and the neural canal in the spinal cord in which neural specific stem cells are present in abundance. Based on these findings, MAb 5B5 might recognize stem cell-associated antigens.

    DOI: 10.1089/hyb.2009.0101

    PubMed

  • Monoclonal Antibody 3G4 Anti-Brg1 Reviewed

    Taro Tachibana

    HYBRIDOMA   29 ( 1 )   81 - 81   2010.02( ISSN:1554-0014

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/hyb.2009.0078.MAb

  • Cytokeratin 8/18 as a new marker of mouse liver preneoplastic lesions Reviewed

    Kakehashi Anna, Kato Ayumi, Inoue Masayo, Ishii Naomi, Okazaki Etsuko, Wei Min, Tachibana Taro, Wanibuchi Hideki

    TOXICOLOGY AND APPLIED PHARMACOLOGY   242 ( 1 )   47 - 55   2010.01( ISSN:0041-008X

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.taap.2009.09.013

  • Dissecting differential gene expression within the circadian neuronal circuit of Drosophila Reviewed

    Nagoshi Emi, Sugino Ken, Kula Ela, Okazaki Etsuko, Tachibana Taro, Nelson Sacha, Rosbash Michael

    NATURE NEUROSCIENCE   13 ( 1 )   60 - U220   2010.01( ISSN:1097-6256

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    DOI: 10.1038/nn.2451

  • Cytokeratin 8/18 as a new marker of mouse liver preneoplastic lesions Reviewed

    Anna Kakehashi, Ayumi Kato, Masayo Inoue, Naomi Ishii, Etsuko Okazaki, Min Wei, Taro Tachibana, Hideki Wanibuchi

    TOXICOLOGY AND APPLIED PHARMACOLOGY   242 ( 1 )   47 - 55   2010.01( ISSN:0041-008X

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    To search for a reliable biomarker of preneoplastic lesions arising early in mouse hepatocarcinogenesis the proteomes of microdissected basophilic foci, hepatocellular adenomas (HCAs), carcinomas (HCCs) and normal-appearing liver of B6C3F1 mice initiated with diethylnitrosamine (DEN) were analysed on anionic (Q10) surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) ProteinChip arrays. Significant overexpression of cytokeratin 8 (CK8; m/z 54, 565), cytokeratin 18 (CK18; m/z 47,538) proteins was found in basophilic foci as well as in HCAs and HCCs. Furthermore, immunohistochemistry demonstrated profound overexpression of CK8 and CK18 proteins (CK8/18) in all basophilic foci, mixed cell type foci, HCAs and HCCs in B6CF1 and C57BL/6J mice initiated with DEN. A strong correlation between CK8/18-positive foci development and multiplicity of liver tumors in 1360171 and C57Bl/6J mice was further observed. Moreover, formation of CK8 and CK18 complexes due to CK8 phosphorylation at Ser73 and Ser431 was found to be strongly associated with neoplastic transformation of mice liver basophilic foci. Elevation of CK8/18 was strongly correlated with induction of cell proliferation in basophilic foci and tumors. In conclusion, our data imply that CK8/18 is a novel reliable marker of preneoplastic lesions arising during mouse hepatocarcinogenesis which might be used for prediction of tumor development and evaluation of environmental agents as well as drugs and food additives using mouse liver tests. (C) 2009 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.taap.2009.09.013

    PubMed

  • Molecular characterization and expression of the low-density lipoprotein receptor-related protein-10, a new member of the LDLR gene family Reviewed

    Young-Hee Jeong, Kayoko Ishikawa, Yoshimi Someya, Akemi Hosoda, Tomohiko Yoshimi, Chikako Yokoyama, Sumiko Kiryu-Seo, Man-Jong Kang, Taro Tchibana, Hiroshi Kiyama, Tomoe Fukumura, Dong-Ho Kim, Shigeru Saeki

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   391 ( 1 )   1110 - 1115   2010.01( ISSN:0006-291X

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    We report the characterization of a new member of the low-density lipoprotein receptor (LDLR) gene family designated LRP10. Human LRP10 cDNA encodes a 1905 amino acid type I membrane protein consisting of five functional domains characteristic of the LDLR gene family. CHO-ldlA7 cells transfected with human LRP10 cDNA bound LDLR-associated protein, but not beta-VLDL and HDL. Human LRP10 transcripts were primarily found in the brain, muscle and heart. in situ hybridization of the rat brain showed that the transcripts were intensely present in the cerebral cortex, hippocampus, choroid plexus, ependyma and granular layer. In the developing rat brain, transcript levels gradually increased from postnatal day 1 to 20. Immunofluorescence analysis indicated that LRP10 was observed in the ventricular zone of the embryonic day 14.5 mouse cerebral cortex. The present studies suggest that LRP10 may play a significant role in the brain physiology other than lipoprotein metabolism. (C) 2009 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2009.12.033

  • Dissecting differential gene expression within the circadian neuronal circuit of Drosophila Reviewed

    Emi Nagoshi, Ken Sugino, Ela Kula, Etsuko Okazaki, Taro Tachibana, Sacha Nelson, Michael Rosbash

    NATURE NEUROSCIENCE   13 ( 1 )   60 - U220   2010.01( ISSN:1097-6256

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    Behavioral circadian rhythms are controlled by a neuronal circuit consisting of diverse neuronal subgroups. To understand the molecular mechanisms underlying the roles of neuronal subgroups within the Drosophila circadian circuit, we used cell-type specific gene-expression profiling and identified a large number of genes specifically expressed in all clock neurons or in two important subgroups. Moreover, we identified and characterized two circadian genes, which are expressed specifically in subsets of clock cells and affect different aspects of rhythms. The transcription factor Fer2 is expressed in ventral lateral neurons; it is required for the specification of lateral neurons and therefore their ability to drive locomotor rhythms. The Drosophila melanogaster homolog of the vertebrate circadian gene nocturnin is expressed in a subset of dorsal neurons and mediates the circadian light response. The approach should also enable the molecular dissection of many different Drosophila neuronal circuits.

    DOI: 10.1038/nn.2451

  • Rat Monoclonal Antibody Specific for Septin 9.

    Tachibana, T., Okazaki, E., Yoshimi, T., Azuma, M., Kakehashi A. and Wanibuchi, H.

    Hybridoma   29   169 - 171   2010

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  • Rat monoclonal antibody specific for the chromatin remodeling factor CHD1.

    Yoshimura, S., Harada, A., Odawara, J., Azuma, M., Okada, S., Nakamura, M., Ohkawa, Y. and Tachibana, T.

    Hybridoma   29   237 - 240   2010

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    Publishing type:Research paper (scientific journal)  

  • Production and Characterization of Monoclonal Antibodies to Mouse Germ Cells.

    Yokoyama, C., Katoh-Fukui, Y., Morohashi, K., Konno, D., Azuma, M. and Tachibana, T.

    Hybridoma   29   53 - 57   2010

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    Publishing type:Research paper (scientific journal)  

  • Production of a Rat Monoclonal Antibody Specific for Dhx9/NDHII/RHA.

    Kotani, M., Harada, A., Odawara, J., Azuma, M., Okada, S., Nishiyama, Y., Nakamura, M., Tachibana, T. and Ohkawa, Y.

    Hybridoma   29   259 - 261   2010

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  • Generation of a Rat Monoclonal Antibody Specific for MyoD.

    Harada, A., Ohkawa, Y., Ao, S., Odawara, J., Okada, S., Azuma, M., Nishiyama, Y., Nakamura, M., and Tachibana, T.

    Hybridoma   29   255 - 258   2010

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    Publishing type:Research paper (scientific journal)  

  • Molecular characterization and expression of the low-density lipoprotein receptor-related protein-10, a new member of the LDLR gene family.

    Jeong, Y-H., Ishikawa, K., Someya, Y., Hosoda, A., Yoshimi, T., Yokoyama, C., et al.,

    Biochem. Biophys. Res. Commun.   391   1110 - 1115   2010

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    Publishing type:Research paper (scientific journal)  

  • Cytokeratin 8/18 as a new marker of mouse liver preneoplastic lesions.

    Kakehashi,A., Kato, A., Inoue, M., Ishii, N., Okazaki, E., Wei, M., Tachibana, T., and Wanibuchi, H.

    Toxicol. Appl. Pharmacol.   242   47 - 55   2010

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  • The mobile FG nucleoporin Nup98 is a cofactor for Crm1-dependent protein export.

    Oka, M., Asally,M., Yasuda, Y., Ogawa, Y., Tachibana,T. and Yoneda,Y.

    Mol.Biol.Cell   21   1885 - 1896   2010

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  • Live imaging system for visualizing nuclear pore complex (NPC) formation during interphase in mammalian cells.

    Iino H, Maeshima K, Nakatomi R, Kose S, Hashikawa T, Tachibana T, Imamoto N.

    Genes Cells   15   647 - 660   2010

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  • Specific Monoclonal Antibody Against the Nuclear Pore Complex Protein, Nup96.

    Mizuguchi C, Oka M, Moriyama T, Tachibana T, Yoneda Y.

    Hybridoma   29   551 - 553   2010

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    Publishing type:Research paper (scientific journal)  

  • Cytokeratin 8/18 as a new marker of mouse liver preneoplastic lesions. Reviewed

    Kakehashi,A., Kato, A., Inoue, M., Ishii, N., Okazaki, E., Wei, M., Tachibana, T., and Wanibuchi, H.

    Toxicol. Appl. Pharmacol.   242   47 - 55   2010

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    Publishing type:Research paper (scientific journal)  

  • The mobile FG nucleoporin Nup98 is a cofactor for Crm1-dependent protein export. Reviewed

    Oka, M., Asally,M., Yasuda, Y., Ogawa, Y., Tachibana,T. and Yoneda,Y.

    Mol.Biol.Cell   21   1885 - 1896   2010

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  • Specific Monoclonal Antibody Against the Nuclear Pore Complex Protein, Nup96. Reviewed

    Mizuguchi C, Oka M, Moriyama T, Tachibana T, Yoneda Y.

    Hybridoma   29   551 - 553   2010

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    Publishing type:Research paper (scientific journal)  

  • Rat monoclonal antibody specific for the chromatin remodeling factor CHD1. Reviewed

    Yoshimura, S., Harada, A., Odawara, J., Azuma, M., Okada, S., Nakamura, M., Ohkawa, Y. and Tachibana, T.

    Hybridoma   29   237 - 240   2010

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    Publishing type:Research paper (scientific journal)  

  • Rat Monoclonal Antibody Specific for Septin 9. Reviewed

    Tachibana, T., Okazaki, E., Yoshimi, T., Azuma, M., Kakehashi A. and Wanibuchi, H.

    Hybridoma   29   169 - 171   2010

     More details

    Publishing type:Research paper (scientific journal)  

  • Production of a Rat Monoclonal Antibody Specific for Dhx9/NDHII/RHA. Reviewed

    Kotani, M., Harada, A., Odawara, J., Azuma, M., Okada, S., Nishiyama, Y., Nakamura, M., Tachibana, T. and Ohkawa, Y.

    Hybridoma   29   259 - 261   2010

     More details

    Publishing type:Research paper (scientific journal)  

  • Production and Characterization of Monoclonal Antibodies to Mouse Germ Cells. Reviewed

    Yokoyama, C., Katoh-Fukui, Y., Morohashi, K., Konno, D., Azuma, M. and Tachibana, T.

    Hybridoma   29   53 - 57   2010

     More details

    Publishing type:Research paper (scientific journal)  

  • Molecular characterization and expression of the low-density lipoprotein receptor-related protein-10, a new member of the LDLR gene family. Reviewed

    Jeong, Y-H., Ishikawa, K., Someya, Y., Hosoda, A., Yoshimi, T., Yokoyama, C., et al.,

    Biochem. Biophys. Res. Commun.   391   1110 - 1115   2010

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    Publishing type:Research paper (scientific journal)  

  • Live imaging system for visualizing nuclear pore complex (NPC) formation during interphase in mammalian cells. Reviewed

    Iino H, Maeshima K, Nakatomi R, Kose S, Hashikawa T, Tachibana T, Imamoto N.

    Genes Cells   15   647 - 660   2010

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    Publishing type:Research paper (scientific journal)  

  • Generation of a Rat Monoclonal Antibody Specific for MyoD. Reviewed

    Harada, A., Ohkawa, Y., Ao, S., Odawara, J., Okada, S., Azuma, M., Nishiyama, Y., Nakamura, M., and Tachibana, T.

    Hybridoma   29   255 - 258   2010

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    Publishing type:Research paper (scientific journal)  

  • 酵母を用いた小型グルコース燃料電池の性能向上 Reviewed

    高野 晃輔, 脇坂 知行, 立花 太郎, 東 雅之

    化学工学会 研究発表講演要旨集   2010   677 - 677   2010

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.11491/scej.2010f.0.677.0

    CiNii Article

  • Production of a Rat Monoclonal Antibody Against Brg1 Reviewed

    Ohkawa Yasuyuki, Harada Akihito, Nakamura Mako, Yoshimura Saori, Tachibana Taro

    HYBRIDOMA   28 ( 6 )   463 - 466   2009.12( ISSN:1554-0014

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/hyb.2009.0041

  • Monoclonal Antibody 6F10 Anti-glyoxalase I (GLO1) Reviewed

    Tachibana Taro

    HYBRIDOMA   28 ( 6 )   467 - 467   2009.12( ISSN:1554-0014

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    DOI: 10.1089/hyb.2009.0063.MAb

  • Generation of A Rat Monoclonal Antibody Specific for Glyoxalase I Reviewed

    Nakadate Yusuke, Mizuguchi Chiaki, Azuma Masayuki, Tachibana Taro

    HYBRIDOMA   28 ( 6 )   447 - 450   2009.12( ISSN:1554-0014

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/hyb.2009.0048

  • Generation of A Rat Monoclonal Antibody Specific for Glyoxalase I Reviewed

    Yusuke Nakadate, Chiaki Mizuguchi, Masayuki Azuma, Taro Tachibana

    HYBRIDOMA   28 ( 6 )   447 - 450   2009.12( ISSN:1554-0014

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    Glyoxalase I (GLO1) is a key enzyme that plays a role in the detoxification of methylglyoxal (MG), a toxic cellular metabolite produced during glycolysis. The present study reports on the preparation and properties of a monoclonal antibody (MAb) directed against mouse GLO1. The antibody was produced by hybridization of mouse myeloma cells with lymph node cells from an immunized rat. The MAb 6F10 specifically recognized GLO1, as evidenced by immunoblotting using a variety of extracts from cultured cells. In immunostaining using MAb 6F10, a diffuse cytoplasmic and nuclear staining pattern was observed. The MAb 6F10 promises to be useful in immunoblotting and immunostaining experiments in various cells and tissues to determine the expression levels of GLO1, as well as to further analyze the biological function of this protein.

    DOI: 10.1089/hyb.2009.0048

    PubMed

  • Production of a Rat Monoclonal Antibody Against Brg1 Reviewed

    Yasuyuki Ohkawa, Akihito Harada, Mako Nakamura, Saori Yoshimura, Taro Tachibana

    HYBRIDOMA   28 ( 6 )   463 - 466   2009.12( ISSN:1554-0014

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    Brm-related gene-1 (Brg1) is a catalytic subunit of the SWI/SNF chromatin remodeling enzyme complex that has ATPase activity. This complex facilitates chromatin remodeling for gene expression by utilizing energy for ATP hydrolysis. It is well known that the SWI/SNF chromatin remodeling enzyme complex is essential for cell differentiation, cell cycle regulation, and embryogenesis. Here we report the establishment of a hybridoma cell line for producing an antibody against Brg1 subunit by the rat medial iliac lymph node method. Immunoblot analysis showed that our antibody can specifically recognize Brg1. It was revealed by immunocytochemistry that Brg1 is located in euchromatin of C2C12 myoblast nuclei. These data suggested this antibody is useful for analyzing molecular function of Brg1 protein in cells.

    DOI: 10.1089/hyb.2009.0041

  • Monoclonal Antibody 6F10 Anti-glyoxalase I (GLO1) Reviewed

    Taro Tachibana

    HYBRIDOMA   28 ( 6 )   467 - 467   2009.12( ISSN:1554-0014

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    DOI: 10.1089/hyb.2009.0063.MAb

  • FM-P34 Glucose fuel battery powered by yeast-analysis of the battery for high performance(Section VIII Fermentation and Microbial Technology) Reviewed

    Azuma Masayuki, Ito Yuichi, Takano Kosuke, Tachibana Taro, Koyama Shinji, Takada Yogo, Wakisaka Tomoyuki

    公益社団法人日本生物工学会 Journal of bioscience and bioengineering   108 ( 1 )   S129 - S130   2009.11( ISSN:13891723

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    CiNii Article

  • Glucose fuel battery powered by yeast-analysis of the battery for high performance Reviewed

    Azuma Masayuki, Ito Yuichi, Takano Kosuke, Tachibana Taro, Koyama Shinji, Takada Yogo, Wakisaka Tomoyuki

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   108   S129 - S130   2009.11( ISSN:1389-1723

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    DOI: 10.1016/j.jbiosc.2009.08.378

  • Glucose fuel battery powered by yeast-analysis of the battery for high performance Reviewed

    Masayuki Azuma, Yuichi Ito, Kosuke Takano, Taro Tachibana, Shinji Koyama, Yogo Takada, Tomoyuki Wakisaka

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   108   S129 - S130   2009.11( ISSN:1389-1723

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jbiosc.2009.08.378

  • FM-P34 Glucose fuel battery powered by yeast-analysis of the battery for high performance(Section VIII Fermentation and Microbial Technology) Reviewed

    Azuma Masayuki, Ito Yuichi, Takano Kosuke, Tachibana Taro, Koyama Shinji, Takada Yogo, Wakisaka Tomoyuki

    Journal of bioscience and bioengineering   108 ( 1 )   S129 - S130   2009.11( ISSN:13891723

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    CiNii Article

  • Generation of Rat Monoclonal Antibodies Specific for Ad4BP/SF-1 Reviewed

    Yokoyama Chikako, Komatsu Tomoko, Ogawa Hidesato, Morohashi Ken-ichirou, Azuma Masayuki, Tachibana Taro

    HYBRIDOMA   28 ( 2 )   113 - 119   2009.04( ISSN:1554-0014

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    DOI: 10.1089/hyb.2008.0084

  • MAbs 1B1, 7D5, and 8G2 Anti-Ad4BP/SF-1 Reviewed

    Tachibana Taro

    HYBRIDOMA   28 ( 2 )   151 - 151   2009.04( ISSN:1554-0014

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    DOI: 10.1089/hyb.2008.0096.MAb

  • Generation of Rat Monoclonal Antibodies Specific for Ad4BP/SF-1

    Chikako Yokoyama, Tomoko Komatsu, Hidesato Ogawa, Ken-ichirou Morohashi, Masayuki Azuma, Taro Tachibana

    HYBRIDOMA   28 ( 2 )   113 - 119   2009.04( ISSN:1554-0014

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    Ad4BP/SF-1 (adrenal4 binding protein/steroidogenic factor-1[NR5A1]) is an essential nuclear receptor required for animal reproduction and endocrine regulation. The present study reports on monoclonal antibodies (MAbs) directed against mouse Ad4BP/SF-1, which were produced by the hybridization of mouse myeloma cells with lymph node cells of an immunized rat. The produced MAbs reacted with both recombinant and endogenous Ad4BP/SF-1. These MAbs will be useful in immunolocalization and immunoblotting experiments conducted on different tissue types to determine the levels of expression of Ad4BP/SF-1 throughout development, as well as further analyses of the biological function and cellular dynamics of this protein.

    DOI: 10.1089/hyb.2008.0084

    PubMed

  • MAbs 1B1, 7D5, and 8G2 Anti-Ad4BP/SF-1 Reviewed

    Taro Tachibana

    HYBRIDOMA   28 ( 2 )   151 - 151   2009.04( ISSN:1554-0014

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    DOI: 10.1089/hyb.2008.0096.MAb

  • The formation of argpyrimidine, a methylglyoxal-arginine adduct, in the nucleus of neural cells Reviewed

    Nakadate Yusuke, Uchida Koji, Shikata Keiji, Yoshimura Saori, Azuma Masayuki, Hirata Tatsumi, Konishi Hiroyuki, Kiyama Hiroshi, Tachibana Taro

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   378 ( 2 )   209 - 212   2009.01( ISSN:0006-291X

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    DOI: 10.1016/j.bbrc.2008.11.028

  • The formation of argpyrimidine, a methylglyoxal-arginine adduct, in the nucleus of neural cells Reviewed

    Yusuke Nakadate, Koji Uchida, Keiji Shikata, Saori Yoshimura, Masayuki Azuma, Tatsumi Hirata, Hiroyuki Konishi, Hiroshi Kiyama, Taro Tachibana

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   378 ( 2 )   209 - 212   2009.01( ISSN:0006-291X

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    Methylglyoxal (MG) is an endogenous metabolite in glycolysis and forms stable adducts primarily with arginine residues of intracellular proteins. The biological role of this modification in cell function is not known. In the present study, we found that a MG-detoxification enzyme glyoxalase I (GLO1) is mainly expressed in the ventricular zone (VZ) at embryonic clay 16 which neural stein and progenitor cells localize. Moreover, immunohistochemical analysis revealed that argpyrimidine, a major MG-arginine adduct, is predominantly produced in cortical plate neurons not VZ during cerebral Cortex development and is exclusively located in the nucleus. Immunoblotting experiment showed that the formation of argpyrimidine occurs oil some nuclear proteins of cortical neurons. To our knowledge, this is first report of the argpyrimidine formation in the nucleus of neuron. These findings Suggest that GLO1, which is dominantly expressed in the embryonic VZ, reduces the intracellular level of MG and Suppresses the formation of argpyrimidine in neural stern and progenitor cells. Argpyrimidine May contribute to the neural differentiation and/or the maintenance of the differentiated state via the modification of nuclear proteins. (C) 2008 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2008.11.028

    PubMed

  • The formation of argpyrimidine, a methylglyoxal-arginine adduct, in the nucleus of neural cells.

    Nakadate, Y., Uchida, K., Shikata, K., Yoshimura, S., Azuma, M., Hirata, T., Konishi, H., Kiyama, H. and Tachibana, T

    Biochem Biophys Res Commun.   378   209 - 212   2009

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  • Generation of Rat Monoclonal Antibodies Specific for Ad4BP/SF-1.

    Yokoyama, C., Komatsu, T., Ogawa, H., Morohashi, K., Azuma, M. and Tachibana, T.

    Hybridoma   28   113 - 119   2009

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  • Production of a Rat Monoclonal Antibody against BRG1.

    Ohkawa, Y., Harada, A., Nakamura, M., Yoshimura, S. and Tachibana, T.

    Hybridoma   28   463 - 466   2009

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  • Generation of a Rat Monoclonal Antibody Specific for Glyoxalase I.

    Nakadate, Y., Mizuguchi, C., Azuma, M. and Tachibana, T.

    Hybridoma   28   447 - 450   2009

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    Publishing type:Research paper (scientific journal)  

  • Gene expression dissection of the circadian neuronal circuit of Drosophila identifies novel circadian genes.

    Nagoshi, E., Sugino, K., Kula, E., Okazaki, E., Tachibana, T. and Nelson, S.

    Nature Neuroscience   13   60 - 68   2009

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  • A Rat Monoclonal Antibody against the Chromatin Remodeling Factor, CHD5.

    Yoshimura, S., Yoshimi, T., Ohkawa, Y., Azuma, M. and Tachibana, T.

    Hybridoma   29   63 - 66   2009

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  • A Rat Monoclonal Antibody against the Chromatin Remodeling Factor, CHD5. Reviewed

    Yoshimura, S., Yoshimi, T., Ohkawa, Y., Azuma, M. and Tachibana, T.

    Hybridoma   29   63 - 66   2009

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  • The formation of argpyrimidine, a methylglyoxal-arginine adduct, in the nucleus of neural cells. Reviewed

    Nakadate, Y., Uchida, K., Shikata, K., Yoshimura, S., Azuma, M., Hirata, T., Konishi, H., Kiyama, H. and Tachibana, T

    Biochem Biophys Res Commun.   378   209 - 212   2009

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  • Production of a Rat Monoclonal Antibody against BRG1. Reviewed

    Ohkawa, Y., Harada, A., Nakamura, M., Yoshimura, S. and Tachibana, T.

    Hybridoma   28   463 - 466   2009

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    Publishing type:Research paper (scientific journal)  

  • Generation of Rat Monoclonal Antibodies Specific for Ad4BP/SF-1. Reviewed

    Yokoyama, C., Komatsu, T., Ogawa, H., Morohashi, K., Azuma, M. and Tachibana, T.

    Hybridoma   28   113 - 119   2009

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    Publishing type:Research paper (scientific journal)  

  • Generation of a Rat Monoclonal Antibody Specific for Glyoxalase I. Reviewed

    Nakadate, Y., Mizuguchi, C., Azuma, M. and Tachibana, T.

    Hybridoma   28   447 - 450   2009

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  • Gene expression dissection of the circadian neuronal circuit of Drosophila identifies novel circadian genes. Reviewed

    Nagoshi, E., Sugino, K., Kula, E., Okazaki, E., Tachibana, T. and Nelson, S.

    Nature Neuroscience   13   60 - 68   2009

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  • Gene expression dissection of the circadian neuronal circuit of Drosophila identifies novel circadian genes. Reviewed

    Nagoshi, E, Sugino, K, Kula, E, Okazaki, E, Tachibana, T, Nelson, S

    Nature Neuroscience   13   60 - 68   2009

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  • Generation and characterization of a monoclonal antibody against NPI-1 subfamily of importin alpha Reviewed

    Tachibana Taro, Sakaguchi Naoko, Miyamoto Yoichi, Sekimoto Toshihiro, Yoneda Yoshihiro, Azuma Masayuki

    HYBRIDOMA   27 ( 4 )   285 - 289   2008.08( ISSN:1554-0014

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    DOI: 10.1089/hyb.2008.0010

  • Monoclonal antibody 2D9 against importin alpha Reviewed

    Tachibana Taro

    HYBRIDOMA   27 ( 4 )   324 - 324   2008.08( ISSN:1554-0014

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    DOI: 10.1089/hyb.2008.0023.MAb

  • Monoclonal antibody 2D9 against importin alpha Reviewed

    Taro Tachibana

    HYBRIDOMA   27 ( 4 )   324 - 324   2008.08( ISSN:1554-0014

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    DOI: 10.1089/hyb.2008.0023.MAb

  • Pituitary homeobox 2 regulates adrenal4 binding protein/steroidogenic factor-1 gene transcription in the pituitary gonadotrope through interaction with the intronic enhancer Reviewed

    Shima Yuichi, Zubair Mohamad, Komatsu Tomoko, Oka Sanae, Yokoyama Chikako, Tachibana Taro, Hjalt Tord A., Drouin Jacques, Morohashi Ken-ichirou

    MOLECULAR ENDOCRINOLOGY   22 ( 7 )   1633 - 1646   2008.07( ISSN:0888-8809

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    DOI: 10.1210/me.2007-0444

  • Pituitary homeobox 2 regulates adrenal4 binding protein/steroidogenic factor-1 gene transcription in the pituitary gonadotrope through interaction with the intronic enhancer Reviewed

    Yuichi Shima, Mohamad Zubair, Tomoko Komatsu, Sanae Oka, Chikako Yokoyama, Taro Tachibana, Tord A. Hjalt, Jacques Drouin, Ken-ichirou Morohashi

    MOLECULAR ENDOCRINOLOGY   22 ( 7 )   1633 - 1646   2008.07( ISSN:0888-8809

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    Ad4BP/SF-1 [adrenal4 binding protein/steroidogenic factor-1 (NR5A1)] is a factor important for animal reproduction and endocrine regulation, and its expression is tightly regulated in the gonad, adrenal gland, ventromedial hypothalamic nucleus, and pituitary gonadotrope. Despite its functional significance in the pituitary, the mechanisms underlying pituitary-specific expression of the gene remain to be uncovered. In this study, we demonstrate by transgenic mouse assays that the pituitary gonadotrope-specific enhancer is localized within the sixth intron of the gene. Functionally, the enhancer recapitulates endogenous Ad4BP/SF-1 expression in the fetal Rathke&apos;s pouch to the adult pituitary gonadotrope. Structurally, the enhancer consists of several elements conserved among animal species. Mutational analyses confirmed the significance of these elements for the enhancer function. One of these elements was able to interact both in vitro and in vivo with Pitx2 (pituitary homeobox 2), demonstrating that pituitary homeobox 2 regulates Ad4BP/SF-1 gene transcription in the pituitary gonadotrope via interaction with the gonadotrope-specific enhancer.

    DOI: 10.1210/me.2007-0444

  • Synthesis and Degradation of Acyl Peptide Using Enzyme from Pseudomonas aeruginosa Reviewed

    Islam Nazneen Naher, Igarashi Koichi, Tachibana Taro, OOSHIMA Hiroshi, AZUMA Masayuki

    公益社団法人日本生物工学会 Journal of bioscience and bioengineering   105 ( 3 )   282 - 287   2008.03( ISSN:13891723

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    The detailed properties of the enzyme from Pseudomonas aeruginosa, which catalyzes the N-acyl linkage between myristic acid and the N-terminal glycine residue of the octapeptide GNAAAARR-NH_2(PKA) in aqueous solution without ATP and CoA, were studied. The substrate specificity for the acyl peptide in the synthetic reaction was examined, and it was found that at least eight amino acid residues are required for the reaction and that the N-terminal glycine residue is not absolutely essential for the reaction because the activity was detected using the octapeptide that has an N-terminal alanine. The activity was also strongly affected by the amino acid sequence because the activity was very weak in the reaction using GARASVLS-NH_2(HIV-1p17^<gag>). The substrate specificity for fatty acids was also examined. In the reactions using lauric acid and decanoic acid, only slight activities were detected; however, those activities were very small compared with the activity in the reaction using myristic acid. In addition, the degradation of myristoyl PKA by the enzyme was detected, although there are only a few reports on demyristoylation. The optimum pH and temperature of the degradation reaction were consistent with those of the synthetic reaction. The degradation reaction was inhibited by divalent cations.

    CiNii Article

  • Synthesis and Degradation of Acyl Peptide Using Enzyme from Pseudomonas aeruginosa Reviewed

    ISLAM Nazneen Naher, IGARASHI Koichi, TACHIBANA Taro, OOSHIMA Hiroshi, AZUMA Masayuki

    Journal of bioscience and bioengineering   105 ( 3 )   282 - 287   2008.03( ISSN:1389-1723

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    The detailed properties of the enzyme from Pseudomonas aeruginosa, which catalyzes the N-acyl linkage between myristic acid and the N-terminal glycine residue of the octapeptide GNAAAARR-NH_2(PKA) in aqueous solution without ATP and CoA, were studied. The substrate specificity for the acyl peptide in the synthetic reaction was examined, and it was found that at least eight amino acid residues are required for the reaction and that the N-terminal glycine residue is not absolutely essential for the reaction because the activity was detected using the octapeptide that has an N-terminal alanine. The activity was also strongly affected by the amino acid sequence because the activity was very weak in the reaction using GARASVLS-NH_2(HIV-1p17^<gag>). The substrate specificity for fatty acids was also examined. In the reactions using lauric acid and decanoic acid, only slight activities were detected; however, those activities were very small compared with the activity in the reaction using myristic acid. In addition, the degradation of myristoyl PKA by the enzyme was detected, although there are only a few reports on demyristoylation. The optimum pH and temperature of the degradation reaction were consistent with those of the synthetic reaction. The degradation reaction was inhibited by divalent cations.

    CiNii Article

  • Synthesis and degradation of acyl peptide using enzyme from Pseudomonas aeruginosa Reviewed

    Islam Nazneen Naher, Igarashi Koichi, Tachibana Taro, Ooshima Hiroshi, Azuma Masayuki

    The Society for Biotechnology, Japan JOURNAL OF BIOSCIENCE AND BIOENGINEERING   105 ( 3 )   282 - 287   2008.03( ISSN:1389-1723

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    The detailed properties of the enzyme from <i>Pseudomonas aeruginosa</i>, which catalyzes the <i>N</i>-acyl linkage between myristic acid and the <i>N</i>-terminal glycine residue of the octapeptide GNAAAARR-NH<sub>2</sub> (PKA) in aqueous solution without ATP and CoA, were studied. The substrate specificity for the acyl peptide in the synthetic reaction was examined, and it was found that at least eight amino acid residues are required for the reaction and that the <i>N</i>-terminal glycine residue is not absolutely essential for the reaction because the activity was detected using the octapeptide that has an <i>N</i>-terminal alanine. The activity was also strongly affected by the amino acid sequence because the activity was very weak in the reaction using GARASVLS-NH<sub>2</sub> (HIV-1p17<sup>gag</sup>). The substrate specificity for fatty acids was also examined. In the reactions using lauric acid and decanoic acid, only slight activities were detected; however, those activities were very small compared with the activity in the reaction using myristic acid. In addition, the degradation of myristoyl PKA by the enzyme was detected, although there are only a few reports on demyristoylation. The optimum pH and temperature of the degradation reaction were consistent with those of the synthetic reaction. The degradation reaction was inhibited by divalent cations.<br>

    DOI: 10.1263/jbb.105.282

    CiNii Article

  • Membrane-anchored growth factor, HB-EGF, on the cell surface targeted to the inner nuclear membrane Reviewed

    Hieda Miki, Isokane Mayumi, Koizumi Michiko, Higashi Chicluru, Tachibana Taro, Shudou Masachika, Taguchi Tomohiko, Hieda Yohki, Higashiyama Shigeki

    JOURNAL OF CELL BIOLOGY   180 ( 4 )   763 - 769   2008.02( ISSN:0021-9525

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    DOI: 10.1083/jcb.200710022

  • Nuclear RNA Export Factor 7 is localized in processing bodies and neuronal RNA granules through interactions with shuttling hnRNPs.

    Katahira, J., Miki, T., Takano, K., Maruhashi, M., Uchikawa, M., Tachibana, T. and Yoneda, Y.

    Nucleic Acids Res.   36   616 - 628   2008.02

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  • Nuclear RNA export factor 7 is localized in processing bodies and neuronal RNA granules through interactions with shuttling hnRNPs Reviewed

    Katahira Jun, Miki Takashi, Takano Keizo, Maruhashi Mitsuji, Uchikawa Masanori, Tachibana Taro, Yoneda Yoshihiro

    NUCLEIC ACIDS RESEARCH   36 ( 2 )   616 - 628   2008.02( ISSN:0305-1048

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    DOI: 10.1093/nar/gkm556

  • Nuclear RNA Export Factor 7 is localized in processing bodies and neuronal RNA granules through interactions with shuttling hnRNPs. Reviewed

    Katahira, J., Miki, T., Takano, K., Maruhashi, M., Uchikawa, M., Tachibana, T. and Yoneda, Y.

    Nucleic Acids Res.   36   616 - 628   2008.02

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  • Nuclear RNA export factor 7 is localized in processing bodies and neuronal RNA granules through interactions with shuttling hnRNPs Reviewed

    Jun Katahira, Takashi Miki, Keizo Takano, Mitsuji Maruhashi, Masanori Uchikawa, Taro Tachibana, Yoshihiro Yoneda

    NUCLEIC ACIDS RESEARCH   36 ( 2 )   616 - 628   2008.02( ISSN:0305-1048

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    The nuclear RNA export factor (NXF) family proteins have been implicated in various aspects of post-transcriptional gene expression. This study shows that mouse NXF7 exhibits heterologous localization, i.e. NXF7 associates with translating ribosomes, stress granules (SGs) and processing bodies (P-bodies), the latter two of which are believed to be cytoplasmic sites of storage, degradation and/or sorting of mRNAs. By yeast two-hybrid screening, a series of heterogeneous nuclear ribonucleoproteins (hnRNPs) were identified as possible binding partners for NXF7. Among them, hnRNP A3, which is believed to be involved in translational control and/or cytoplasmic localization of certain mRNAs, formed a stable complex with NXF7 in vitro. Although hnRNP A3 was not associated with translating ribosomes, it was co-localized with NXF7 in P-bodies. After exposing to oxidative stress, NXF7 trans-localized to SGs, whereas hnRNP A3 did not. In differentiated neuroblastoma Neuro2a cells, NXF7 was co-localized with hnRNP A3 in cell body and neurites. The amino terminal half of NXF7, which was required for stable complex formation with hnRNP A3, coincided with the region required for localization in both P-bodies and neuronal RNA granules. These findings suggest that NXF7 plays a role in sorting, transport and/or storage of mRNAs through interactions with hnRNP A3.

    DOI: 10.1093/nar/gkm556

  • Membrane-anchored growth factor, HB-EGF, on the cell surface targeted to the inner nuclear membrane Reviewed

    Miki Hieda, Mayumi Isokane, Michiko Koizumi, Chicluru Higashi, Taro Tachibana, Masachika Shudou, Tomohiko Taguchi, Yohki Hieda, Shigeki Higashiyama

    JOURNAL OF CELL BIOLOGY   180 ( 4 )   763 - 769   2008.02( ISSN:0021-9525

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    Heparin-binding EGF-like growth factor (HB-EGF) is synthesized as a type I transmembrane protein (proHB-EGF) and expressed on the cell surface. The ectodomain shedding of proHB-EGF at the extracellular region on the plasma membrane yields a soluble EGF receptor ligand and a transmembrane-cytoplasmic fragment (HB-EGF-CTF). The cytoplasmic domain of proHB-EGF (HB-EGF-cyto) interacts with transcriptional repressors to reverse their repressive activities. However, how HB-EGF-cyto accesses transcriptional repressors is yet unknown. The present study demonstrates that, after exposure to shedding stimuli, both HB-EGF-CTF and unshed proHB-EGF translocate to the nuclear envelope. Immunoelectron microscopy and digitonin-permeabilized cells showed that HB-EGF-cyto signals are at the inner nuclear membrane. A short sequence element within the HB-EGF-cyto allows a transmembrane protein to localize to the nuclear envelope. The dominant-active form of Rab5 and Rab11 suppressed nuclear envelope targeting. Collectively, these data demonstrate that membrane-anchored HB-EGF is targeted to the inner nuclear membrane via a retrograde membrane trafficking pathway.

    DOI: 10.1083/jcb.200710022

  • Membrane-anchored growth factor, HB-EGF, on the cell surface targeted to the inner nuclear membrane.

    Hieda, M., Isokane, M., Koizumi, M., Higashi, C., Tachibana, T., Shudou, M., Taguchi, T., Hieda, Y. and Higashiyama, S.

    J.Cell Biol.   180   763 - 769   2008

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  • Synthesis and degradation of acyl peptide using enzyme from Pseudomonas aeruginosa.

    Islam, NN., Igarashi, K., Tachibana, T., Ooshima, H., and Azuma, M.

    J Biosci Bioeng.   105   282 - 287   2008

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  • Pitx2 regulates Ad4BP/SF-1 gene transcription in the pituitary gonadotrope through interaction with the intronic enhancer.

    Shima, Y., Zubair, M., Komatsu, T., Oka, S., Yokoyama, C., Tachibana, T., Hjalt, TA., Drouin, J., Morohashi, KI.

    Mol Endocrinol.   22   1633-1646.   2008

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  • Generation and characterization of a monoclonal antibody against NPI-1 subfamily of importin alpha.

    Tachibana, T., Sakaguchi, N., Miyamoto, Y., Sekimoto,T., Yoneda, Y. and Azuma, M.

    Hybridoma   27   285 - 289   2008

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  • Generation and characterization of a monoclonal antibody against NPI-1 subfamily of importin alpha. Reviewed

    Tachibana, T., Sakaguchi, N., Miyamoto, Y., Sekimoto,T., Yoneda, Y. and Azuma, M.

    Hybridoma   27   285 - 289   2008

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  • Synthesis and degradation of acyl peptide using enzyme from Pseudomonas aeruginosa. Reviewed

    Islam, NN., Igarashi, K., Tachibana, T., Ooshima, H., and Azuma, M.

    J Biosci Bioeng.   105   282 - 287   2008

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  • Pitx2 regulates Ad4BP/SF-1 gene transcription in the pituitary gonadotrope through interaction with the intronic enhancer. Reviewed

    Shima, Y., Zubair, M., Komatsu, T., Oka, S., Yokoyama, C., Tachibana, T., Hjalt, TA., Drouin, J., Morohashi, KI.

    Mol Endocrinol.   22   1633-1646.   2008

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    Publishing type:Research paper (scientific journal)  

  • Membrane-anchored growth factor, HB-EGF, on the cell surface targeted to the inner nuclear membrane. Reviewed

    Hieda, M., Isokane, M., Koizumi, M., Higashi, C., Tachibana, T., Shudou, M., Taguchi, T., Hieda, Y. and Higashiyama, S.

    J.Cell Biol.   180   763 - 769   2008

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  • Pitx2 regulates Ad4BP/SF-1 gene transcription in the pituitary gonadotrope through interaction with the intronic enhancer. Reviewed

    Shima, Y, Zubair, M, Komatsu, T, Oka, S, Yokoyama, C, Tachibana, T, Hjalt, TA, Drouin, J, Morohashi, KI

    Mol Endocrinol.   22   1633-1646.   2008

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  • Generation and characterization of a monoclonal antibody against NPI-1 subfamily of importin alpha. Reviewed

    Tachibana, T, Sakaguchi, N, Miyamoto, Y, Sekimoto,T, Yoneda, Y, Azuma, M

    Hybridoma   27   285 - 289   2008

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  • Identification of peripherin as a Akt substrate in neurons Reviewed

    Konishi Hiroyuki, Namikawa Kazuhiko, Shikata Keiji, Kobatake Yuji, Tachibana Taro, Kiyama Hiroshi

    JOURNAL OF BIOLOGICAL CHEMISTRY   282 ( 32 )   23491 - 23499   2007.08( ISSN:0021-9258

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    DOI: 10.1074/jbc.M611703200

  • Identification of peripherin as a Akt substrate in neurons Reviewed

    Hiroyuki Konishi, Kazuhiko Namikawa, Keiji Shikata, Yuji Kobatake, Taro Tachibana, Hiroshi Kiyama

    JOURNAL OF BIOLOGICAL CHEMISTRY   282 ( 32 )   23491 - 23499   2007.08( ISSN:0021-9258

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    Activation of Akt-mediated signaling pathways is crucial for survival and regeneration of injured neurons. In this study, we attempted to identify novel Akt substrates by using an antibody that recognized a consensus motif phosphorylated by Akt. PC12 cells that overexpressed constitutively active Akt were used. Using two-dimensional PAGE, we identified protein spots that exhibited increased immunostaining of the antibody. Mass spectrometry revealed several major spots as the neuronal intermediate filament protein, peripherin. Using several peripherin fragments, the phosphorylation site was determined as Ser(66) in its head domain in vitro. Furthermore, a co-immunoprecipitation experiment revealed that Akt interacted with the head domain of peripherin in HEK 293T cells. An antibody against phosphorylated peripherin was raised, and induction of phosphorylated peripherin was observed not only in Akt-activated cultured cells but also in nerve-injured hypoglossal motor neurons. These results suggest that peripherin is a novel substrate for Akt in vivo and that its phosphorylation may play a role in motor nerve regeneration.

    DOI: 10.1074/jbc.M611703200

    PubMed

  • Short-chain 3-ketoceramides, strong apoptosis inducers against human leukemia HL-60 cells Reviewed

    Azuma Hideki, Ijichi So, Kataoka Mayuko, Masuda Akira, Izumi Takayuki, Yoshimoto Tetsuya, Tachibana Taro

    BIOORGANIC & MEDICINAL CHEMISTRY   15 ( 8 )   2860 - 2867   2007.04( ISSN:0968-0896

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    DOI: 10.1016/j.bmc.2007.02.008

  • Short-chain 3-ketoceramides, strong apoptosis inducers against human leukemia HL-60 cells Reviewed

    Hideki Azuma, So Ijichi, Mayuko Kataoka, Akira Masuda, Takayuki Izumi, Tetsuya Yoshimoto, Taro Tachibana

    BIOORGANIC & MEDICINAL CHEMISTRY   15 ( 8 )   2860 - 2867   2007.04( ISSN:0968-0896

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    Ceramides act as a second messenger of the apoptotic signaling process. The allylic alcohol portion comprising the C-3, C-4, and C-5 carbons is essential for this function. The suggestion has been made that this alcohol moiety is oxidized in mitochondria to a carbonyl moiety, with the generation of reactive oxygen species. However, there is no established precedent for the apoptotic performance of 3-ketoceramides thus presumed. In this work, we have synthesized three different types of short-chain 3-ketoceramides, that is, (2S,4E)-2-acetylamino-3-oxo-4-octadecen-1-ol (A), (2S,4E,6E)-2-acetylamino-3-oxo-4,6-octadecadien-1-ol (B), and (2S,4E)-2-acetylamino-1-methoxy-3-oxo-4-octadecene (C), and demonstrated that these 3-ketoceramides are capable of inducing effective apoptosis in human leukemia HL-60 cells. In particular, the two monoenoic compounds, A and C, are far more powerful than the corresponding alcoholic analogue, N-acetyl-D-erythro-sphingosine. Observations of DNA fragmentation, caspase-3 activation, and cytochrome c release from mitochondria provide substantiated evidence for mitochondrial apoptosis and the effects of exogenous glutathione on these phenomena are also discussed. (c) 2007 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.bmc.2007.02.008

    PubMed

  • Myogenin and the SWI/SNF ATPase Brg1 maintain myogenic gene expression at different stages of skeletal myogenesis Reviewed

    Ohkawa Yasuyuki, Yoshimura Saori, Higashi Chiduru, Marfella Concetta G. A., Dacwag Caroline S., Tachibana Taro, Imbalzano Anthony N.

    JOURNAL OF BIOLOGICAL CHEMISTRY   282 ( 9 )   6564 - 6570   2007.03( ISSN:0021-9258

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    DOI: 10.1074/jbc.M608898200

  • Myogenin and the SWI/SNF ATPase Brg1 maintain myogenic gene expression at different stages of skeletal myogenesis Reviewed

    Yasuyuki Ohkawa, Saori Yoshimura, Chiduru Higashi, Concetta G. A. Marfella, Caroline S. Dacwag, Taro Tachibana, Anthony N. Imbalzano

    JOURNAL OF BIOLOGICAL CHEMISTRY   282 ( 9 )   6564 - 6570   2007.03( ISSN:0021-9258

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    Many studies have examined transcriptional regulation during the initiation of skeletal muscle differentiation; however, there is less information regarding transcriptional control during adult myogenesis and during the maintenance of the differentiated state. MyoD and the mammalian SWI/SNF chromatinremodeling enzymes containing the Brg1 ATPase are necessary to induce myogenesis in cell culture models and in developing embryonic tissue, whereas myogenin and Brg1 are critical for the expression of the late genes that induce terminal muscle differentiation. Here, we demonstrate that myogenin also binds to its own promoter during the late stages of embryonic muscle development. As is the case during embryonic myogenesis, MyoD and Brg1 co-localize to the myogenin promoter in primary adult muscle satellite cells. However, in mature myofibers, myogenin and Brg1 are preferentially co-localized to the myogenin promoter. Thus, the myogenin promoter is occupied by different myogenic factors at different times of myogenesis. The relevance of myogenin in the continued expression from its own promoter is demonstrated in culture, where we show that myogenin, in the absence of MyoD, is capable of maintaining its own expression by recruiting the Brg1 ATPase to modify promoter chromatin structure and facilitate myogenin expression. Finally, we utilized in vivo electroporation to demonstrate that Brg1 is required for the continued production of the myogenin protein in newborn skeletal muscle tissue. These findings strongly suggest that the skeletal muscle phenotype is maintained by myogenin and the continuous activity of Brg1-based SWI/SNF chromatin-remodeling enzymes.

    DOI: 10.1074/jbc.M608898200

  • Identification of peripherin as a novel AKT substrate in neurons.

    J. Biol. Chem.   282   23491 - 23499   2007

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  • Short&#8211;chain 3-ketoceramides, strong apoptosis inducers against human leukemia HL-60 cells.

    Bioorg. Med. Chem.   15   2860 - 2867   2007

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  • Myogenin and the SWI/SNF atpase BRG1 maintain myogenic gene expression at different stages of skeletal myogenesis.

    J. Biol. Chem.   282   6564 - 6570   2007

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  • Identification of peripherin as a novel AKT substrate in neurons. Reviewed

    J. Biol. Chem.   282   23491 - 23499   2007

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    Publishing type:Research paper (scientific journal)  

  • Short–chain 3-ketoceramides, strong apoptosis inducers against human leukemia HL-60 cells. Reviewed

    Bioorg. Med. Chem.   15   2860 - 2867   2007

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  • Myogenin and the SWI/SNF atpase BRG1 maintain myogenic gene expression at different stages of skeletal myogenesis. Reviewed

    J. Biol. Chem.   282   6564 - 6570   2007

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  • Cell-cycle-dependent dynamics of nuclear pores: pore-free islands and lamins Reviewed

    Maeshima Kazuhiro, Yahata Kazuhide, Sasaki Yoko, Nakatomi Reiko, Tachibana Taro, Hashikawa Tsutomu, Imamoto Fumio, Imamoto Naoko

    JOURNAL OF CELL SCIENCE   119 ( 21 )   4442 - 4451   2006.11( ISSN:0021-9533

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    DOI: 10.1242/jcs.03207

  • Cell-cycle-dependent dynamics of nuclear pores: pore-free islands and lamins

    Kazuhiro Maeshima, Kazuhide Yahata, Yoko Sasaki, Reiko Nakatomi, Taro Tachibana, Tsutomu Hashikawa, Fumio Imamoto, Naoko Imamoto

    JOURNAL OF CELL SCIENCE   119 ( 21 )   4442 - 4451   2006.11( ISSN:0021-9533

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    Nuclear pores are sophisticated gateways on the nuclear envelope that control macromolecular transport between the cytoplasm and nucleoplasm. So far the structural and functional aspects of nuclear pores have been extensively studied, but their distribution and density, which might reflect nuclear organization and function, remain unknown. Here, we report the cell-cycle-dependent dynamics of nuclear pores. Large distinct subdomains lacking nuclear pores are present on the nuclear surface of HeLaS3 cells in early cell-cycle stages. Such 'pore-free islands' gradually become dispersed in G1-S phase. Surprisingly, the islands are enriched with inner nuclear membrane proteins lamin A/C and emerin, but exclude lamin B. Lamin-A/C-enriched pore-free islands were also observed in human normal diploid fibroblasts and several cell lines, showing the generality of this phenomenon. Knockdown and ectopic expression analyses demonstrated that lamin A/C, but not emerin, plays an essential structural and regulatory role in the nuclear pore distribution and the formation of pore-free islands. These data thus provide strong evidence that the dynamics of nuclear pores are regulated by the reorganization of inner nuclear structures.

    DOI: 10.1242/jcs.03207

  • Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation Reviewed

    Uchimura Yasuhiro, Ichimura Takaya, Uwada Junsuke, Tachibana Taro, Sugahara Satoko, Nakao Mitsuyoshi, Saitoh Hisato

    JOURNAL OF BIOLOGICAL CHEMISTRY   281 ( 32 )   23180 - 23190   2006.08( ISSN:0021-9258

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    DOI: 10.1074/jbc.M602280200

  • Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation. Reviewed

    Yasuhiro Uchimura, Takaya Ichimura, Junsuke Uwada, Taro Tachibana, Satoko Sugahara, Mitsuyoshi Nakao, Hisato Saitoh

    The Journal of biological chemistry   281 ( 32 )   23180 - 90   2006.08( ISSN:0021-9258

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Small ubiquitin-related modifiers, SUMO-2/3 and SUMO-1, are involved in gene regulation and nuclear structures. However, little is known about the roles of SUMO, in heterochromatin formation of mammalian cells. Here we demonstrate that SUMOs directly interact with human MCAF1, which forms complexes with either the methyl-CpG-binding protein MBD1 or SETDB1, which trimethylates histone H3 at lysine 9 (H3-K9) in the presence of MCAF1. Modification of MBD1 with either SUMO-2/3 or SUMO-1 facilitated the interaction between MBD1 and MCAF1, suggesting that SUMOylation links the methylation of DNA and histones. In a cultured human cell line, SUMOs were localized in MBD1- and MCAF1-containing heterochromatin regions that were enriched in trimethyl-H3-K9 and the heterochromatin proteins HP1beta and HP1gamma. Specific knockdown of either SUMO-2/3 or SUMO-1 induced dissociation of MCAF1, trimethyl-H3-K9, and the HP1 proteins from the MBD1-containing heterochromatin foci, suggesting a requirement for SUMOs for heterochromatin assembly. These findings provide insights into the roles of SUMOylation in the regulation of heterochromatin formation and gene silencing.

    PubMed

  • Unique anti-apoptotic activity of EAAC1 in injured motor neurons Reviewed

    Kiryu-Seo Sumiko, Gamo Kazushige, Tachibana Taro, Tanaka Kohichi, Kiyama Hiroshi

    EMBO JOURNAL   25 ( 14 )   3411 - 3421   2006.07( ISSN:0261-4189

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/sj.emboj.7601225

  • The microphthalmia-associated transcription factor requires SWI/SNF enzymes to activate melanocyte-specific genes Reviewed

    de la Serna Ivana L., Ohkawa Yasuyuki, Higashi Chiduru, Dutta Chaitali, Osias Jules, Kommajosyula Naveen, Tachibana Taro, Imbalzano Anthony N.

    JOURNAL OF BIOLOGICAL CHEMISTRY   281 ( 29 )   20233 - 20241   2006.07( ISSN:0021-9258

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M512052200

  • The microphthalmia-associated transcription factor requires SWI/SNF enzymes to activate melanocyte-specific genes

    Ivana L. de la Serna, Yasuyuki Ohkawa, Chiduru Higashi, Chaitali Dutta, Jules Osias, Naveen Kommajosyula, Taro Tachibana, Anthony N. Imbalzano

    JOURNAL OF BIOLOGICAL CHEMISTRY   281 ( 29 )   20233 - 20241   2006.07( ISSN:0021-9258

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    The microphthalmia transcription factor (Mitf) activates melanocyte-specific gene expression, is critical for survival and proliferation of melanocytes during development, and has been described as an oncogene in malignant melanoma. SWI/SNF complexes are ATP-dependent chromatin-remodeling enzymes that play a role in many developmental processes. To determine the requirement for SWI/SNF enzymes in melanocyte differentiation, we introduced Mitf into fibroblasts that inducibly express dominant negative versions of the SWI/SNF ATPases, Brahma or Brahma-related gene 1 (BRG1). These dominant negative SWI/SNF components have been shown to inhibit gene activation events that normally require SWI/SNF enzymes. We found that Mitf-mediated activation of a subset of endogenous melanocyte-specific genes required SWI/SNF enzymes but that cell-cycle regulation occurred independently of SWI/SNF function. Activation of tyrosinase-related protein 1, a melanocyte-specific gene, correlated with SWI/SNF-dependent changes in chromatin accessibility at the endogenous locus. Both BRG1 and Mitf could be localized to the tyrosinase-related protein 1 and tyrosinase promoters by chromatin immunoprecipitation, whereas immunofluorescence and immunoprecipitation experiments indicate that Mitf and BRG1 co-localized in the nucleus and physically interacted. Together these results suggest that Mitf can recruit SWI/SNF enzymes to melanocyte- specific promoters for the activation of gene expression via induced changes in chromatin structure at endogenous loci.

    DOI: 10.1074/jbc.M512052200

  • MAb 1B6, 2A11, 6A12, 7E11, and 8A12 anti-human Nup 62 Reviewed

    Tachibana T

    HYBRIDOMA   25 ( 3 )   173 - 173   2006.06( ISSN:1554-0014

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  • MAb 1B6, 2A11, 6A12, 7E11, and 8A12 anti-human Nup 62 Reviewed

    Tachibana T

    HYBRIDOMA   25 ( 3 )   173 - 173   2006.06( ISSN:1554-0014

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    Publishing type:Research paper (scientific journal)   Kind of work:Single Work  

  • MAb 1B6, 2A11, 6A12, 7E11, and 8A12 anti-human Nup 62 Reviewed

    T Tachibana

    HYBRIDOMA   25 ( 3 )   173 - 173   2006.06( ISSN:1554-0014

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  • In situ SUMOylation analysis reveals a modulatory role of RanBP2 in the nuclear rim and PML bodies. Reviewed

    Saitoh N, Uchimura Y, Tachibana T, Sugahara S, Saitoh H, Nakao M

    Experimental cell research   312 ( 8 )   1418 - 30   2006.05( ISSN:0014-4827

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    PubMed

  • In situ SUMOylation analysis reveals a modulatory role of RanBP2 in the nuclear rim and PML bodies Reviewed

    Saitoh N, Uchimura Y, Tachibana T, Sugahara S, Saitoh H, Nakao M

    EXPERIMENTAL CELL RESEARCH   312 ( 8 )   1418 - 1430   2006.05( ISSN:0014-4827

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.yexcr.2006.01.013

  • In situ SUMOylation analysis reveals a modulatory role of RanBP2 in the nuclear rim and PML bodies

    N Saitoh, Y Uchimura, T Tachibana, S Sugahara, H Saitoh, M Nakao

    EXPERIMENTAL CELL RESEARCH   312 ( 8 )   1418 - 1430   2006.05( ISSN:0014-4827

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    SUMO modification plays a critical role in a number of cellular functions including nucleocytoplasmic transport, gene expression, cell cycle and formation of subnuclear structures such as promyelocytic leukemia (PML) bodies. In order to identify the sites where SUMOylation takes place in the cell, we developed an in situ SUMOylation assay using a semi-intact cell system and subsequently combined it with siRNA-based knockdown of nucleoporin RanBP2, also known as Nup358, which is one of the known SUMO E3 proteins. With the in situ SUMOylation assay, we found that both nuclear rim and PML bodies, besides mitotic apparatuses, are major targets for active SUMOylation. The ability to analyze possible SUMO conjugation sites would be a valuable tool to investigate where SUMO E3-like activities and/or SUMO substrates exist in the cell. Specific knockdown of RanBP2 completely abolished SUMOylation along the nuclear rim and dislocated RanGAP1 from the nuclear pore complexes. interestingly, the loss of RanBP2 markedly reduced the number of PML bodies, in contrast to other, normal-appearing nuclear compartments including the nuclear lamina, nucleolus and chromatin, suggesting a novel link between RanBP2 and PML bodies. SUMOylation facilitated by RanBP2 at the nuclear rim may be a key step for the formation of a particular subnuclear organization. Our data imply that SUMO E3 proteins like RanBP2 facilitate spatio-temporal SUMOylation for certain nuclear structure and function. (c) 2006 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.yexcr.2006.01.013

  • Functional analysis of nuclear pore complex protein Nup62/p62 using monoclonal antibodies. Reviewed

    Fukuhara T, Sakaguchi N, Katahira J, Yoneda Y, Ogino K, Tachibana T

    Hybridoma (2005)   25 ( 2 )   51 - 9   2006.04( ISSN:1554-0014

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/hyb.2006.25.51

    PubMed

  • Functional analysis of nuclear pore complex protein Nup62/p62 using monoclonal antibodies Reviewed

    Fukuhara T, Sakaguchi N, Katahira J, Yoneda Y, Ogino K, Tachibana T

    HYBRIDOMA   25 ( 2 )   51 - 59   2006.04( ISSN:1554-0014

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  • Functional analysis of nuclear pore complex protein Nup62/p62 using monoclonal antibodies Reviewed

    Fukuhara T, Sakaguchi N, Katahira J, Yoneda Y, Ogino K, Tachibana T

    HYBRIDOMA   25 ( 2 )   51 - 59   2006.04( ISSN:1554-0014

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  • Functional analysis of nuclear pore complex protein Nup62/p62 using monoclonal antibodies Reviewed

    T Fukuhara, N Sakaguchi, J Katahira, Y Yoneda, K Ogino, T Tachibana

    HYBRIDOMA   25 ( 2 )   51 - 59   2006.04( ISSN:1554-0014

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    The nuclear pore complex (NPC) is an enormous structure embedded in the double membrane of the nuclear envelope that acts as a passageway for nucleocytoplasmic transport. The vertebrate NPC is comprised of about 30 unique proteins. Nup62/p62, a major component of the NPC, has been reported to interact directly with several nuclear transport factors, including importin-beta and NTF2. However, it has not been shown how the interaction of Nup62/p62 with transport factors is involved in nucleocytoplasmic transport. The present study reports on the preparation of monoclonal antibodies (MAbs) directed against human Nup62/p62 and a functional analysis of Nup62/p62 using antibodies in living cells. Hybridomas producing the antibodies were produced by the hybridization of mouse myeloma cells with medial iliac lymph node cells from an immunized rat. These MAbs specifically recognized Nup62/p62 as evidenced by immunoblotting analysis using a nuclear membrane fraction. In the immunostaining using MAbs, a punctuate nuclear rim staining pattern was observed. Moreover, cytoplasmic injected-anti-Nup62/p62 MAbs were rapidly targeted to the nuclear pore of cultured cells and some of them inhibited normal cell division, causing the formation of abnormal nuclei. The antibodies described in this study provide the means for immunochemical analyses of the NPC protein Nup62/p62 in mammalian cells, and represent useful molecular tools that should permit a better understanding of the biological roles and cellular dynamics of this protein in nucleocytoplasmic transport, cell division, and nuclear organization.

  • Lipase-catalyzed preparation of optically active 1 '-acetoxychavicol acetates and their structure-activity relationships in apoptotic activity against human leukemia HL-60 cells Reviewed

    Azuma H, Miyasaka K, Yokotani T, Tachibana T, Kojima-Yuasa A, Matsui-Yuasa I, Ogino K

    BIOORGANIC & MEDICINAL CHEMISTRY   14 ( 6 )   1811 - 1818   2006.03( ISSN:0968-0896

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bmc.2005.10.029

  • Identification of peripherin as a novel AKT substrate in neurons.

    J. Biol. Chem.   282   23491 - 23499   2006

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  • Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation.

    J. Biol. Chem.   281   23180 - 23190   2006

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    Publishing type:Research paper (scientific journal)  

  • Cell cycle-dependent dynamics of nuclear pore: pore-free islands and lamins.

    J. Cell Sci.   119   4442 - 4451   2006

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    Publishing type:Research paper (scientific journal)  

  • Unique anti-apoptotic activity of EAAC1 in injured motor neurons.

    EMBO J.   25   3411 - 3421   2006

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    Publishing type:Research paper (scientific journal)  

  • The microphthalmia-associated transcription factor (MITF) requires SWI/SNF enzymes to activate melanocyte specific genes.

    J. Biol. Chem.   281   20233 - 20241   2006

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    Publishing type:Research paper (scientific journal)  

  • In situ SUMOylation analysis reveals a modulatory role of RanBP2 in the nuclear rim and PML bodies.

    Exp. Cell Res.   312   1418 - 1430   2006

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    Publishing type:Research paper (scientific journal)  

  • Functional Analysis of the Nuclear Pore Complex Protein Nup62/p62 Using Monoclonal Antibodies

    Hybridoma   25   51 - 59   2006

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    Publishing type:Research paper (scientific journal)  

  • Cell cycle-dependent dynamics of nuclear pore: pore-free islands and lamins. Reviewed

    J. Cell Sci.   119   4442 - 4451   2006

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  • Identification of peripherin as a novel Akt substrate in neurons Reviewed

    Konishi Hiroyuki, Namikawa Kazuhiko, Shikata Keiji, Kobatake Yuji, Tachibana Taro, Kiyama Hiroshi

    NEUROSCIENCE RESEARCH   55   S177 - S177   2006( ISSN:0168-0102

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  • Unique anti-apoptotic activity of EAAC1 in injured motor neurons. Reviewed

    EMBO J.   25   3411 - 3421   2006

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    Publishing type:Research paper (scientific journal)  

  • The microphthalmia-associated transcription factor (MITF) requires SWI/SNF enzymes to activate melanocyte specific genes. Reviewed

    J. Biol. Chem.   281   20233 - 20241   2006

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    Publishing type:Research paper (scientific journal)  

  • Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation. Reviewed

    J. Biol. Chem.   281   23180 - 23190   2006

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    Publishing type:Research paper (scientific journal)  

  • In situ SUMOylation analysis reveals a modulatory role of RanBP2 in the nuclear rim and PML bodies. Reviewed

    Exp. Cell Res.   312   1418 - 1430   2006

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    Publishing type:Research paper (scientific journal)  

  • Identification of peripherin as a novel AKT substrate in neurons. Reviewed

    J. Biol. Chem.   282   23491 - 23499   2006

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    Publishing type:Research paper (scientific journal)  

  • Identification of peripherin as a novel Akt substrate in neurons Reviewed

    Konishi Hiroyuki, Namikawa Kazuhiko, Shikata Keiji, Kobatake Yuji, Tachibana Taro, Kiyama Hiroshi

    NEUROSCIENCE RESEARCH   55   S177 - S177   2006( ISSN:0168-0102

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    Publishing type:Research paper (scientific journal)  

  • Functional Analysis of the Nuclear Pore Complex Protein Nup62/p62 Using Monoclonal Antibodies Reviewed

    Hybridoma   25   51 - 59   2006

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    Publishing type:Research paper (scientific journal)  

  • Unique anti-apoptotic activity of EAAC1 in injured motor neurons. Reviewed

    EMBO J.   25   3411 - 3421   2006

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    Publishing type:Research paper (scientific journal)  

  • Identification of peripherin as a novel Akt substrate in neurons Reviewed

    Hiroyuki Konishi, Kazuhiko Namikawa, Keiji Shikata, Yuji Kobatake, Taro Tachibana, Hiroshi Kiyama

    NEUROSCIENCE RESEARCH   55   S177 - S177   2006( ISSN:0168-0102

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  • Specific monoclonal antibody against the nuclear pore complex protein, Nup98 Reviewed

    Fukuhara T, Ozaki T, Shikata K, Katahira J, Yoneda Y, Ogino K, Tachibana T

    HYBRIDOMA   24 ( 5 )   244 - 247   2005.10( ISSN:1554-0014

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  • MAb 2H10, anti-Nup98 Reviewed

    Tachibana T

    HYBRIDOMA   24 ( 5 )   272 - 272   2005.10( ISSN:1554-0014

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    Publishing type:Research paper (scientific journal)   Kind of work:Single Work  

  • Specific monoclonal antibody against the nuclear pore complex protein, Nup98 Reviewed

    Fukuhara T, Ozaki T, Shikata K, Katahira J, Yoneda Y, Ogino K, Tachibana T

    HYBRIDOMA   24 ( 5 )   244 - 247   2005.10( ISSN:1554-0014

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  • MAb 2H10, anti-Nup98 Reviewed

    Tachibana T

    HYBRIDOMA   24 ( 5 )   272 - 272   2005.10( ISSN:1554-0014

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    Publishing type:Research paper (scientific journal)   Kind of work:Single Work  

  • Specific monoclonal antibody against the nuclear pore complex protein, nup98. Reviewed

    Fukuhara T, Ozaki T, Shikata K, Katahira J, Yoneda Y, Ogino K, Tachibana T

    Hybridoma (2005)   24 ( 5 )   244 - 7   2005.10( ISSN:1554-0014

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/hyb.2005.24.244

    PubMed

  • MAb 2H10, anti-Nup98 Reviewed

    T Tachibana

    HYBRIDOMA   24 ( 5 )   272 - 272   2005.10( ISSN:1554-0014

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  • Specific monoclonal antibody against the nuclear pore complex protein, Nup98 Reviewed

    T Fukuhara, T Ozaki, K Shikata, J Katahira, Y Yoneda, K Ogino, T Tachibana

    HYBRIDOMA   24 ( 5 )   244 - 247   2005.10( ISSN:1554-0014

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    Nup98 is a component of nuclear pore complexes, which are large protein assemblies embedded in the nuclear envelope. Previous studies have shown that Nup98 interacts with several transport factors and plays a critical part in nuclear trafficking. However, the mechanism by which Nup98 contributes to nuclear trafficking is not clear. The present study reports on the preparation of a monoclonal antibody (MAb) directed against human Nup98. The antibody was produced by the hybridization of mouse myeloma cells with lymph node cells from an immunized rat. This antibody, MAb 21110, specifically recognized Nup98, as evidenced by immunoblotting using a nuclear membrane fraction. In immunostaining using MAb 21110, a punctuate nuclear rim staining pattern was observed. This MAb will be useful in immunoblotting and immunolocalization experiments in various cells and tissues, as well as further analyses of the biological function and cellular dynamics of this protein.

    DOI: 10.1089/hyb.2005.24.244

    PubMed

  • Specific monoclonal antibody against the nuclear pore complex protein, Nup98 Reviewed

    T Fukuhara, T Ozaki, K Shikata, J Katahira, Y Yoneda, K Ogino, T Tachibana

    HYBRIDOMA   24 ( 5 )   244 - 247   2005.10( ISSN:1554-0014

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    Publishing type:Research paper (scientific journal)  

    Nup98 is a component of nuclear pore complexes, which are large protein assemblies embedded in the nuclear envelope. Previous studies have shown that Nup98 interacts with several transport factors and plays a critical part in nuclear trafficking. However, the mechanism by which Nup98 contributes to nuclear trafficking is not clear. The present study reports on the preparation of a monoclonal antibody (MAb) directed against human Nup98. The antibody was produced by the hybridization of mouse myeloma cells with lymph node cells from an immunized rat. This antibody, MAb 21110, specifically recognized Nup98, as evidenced by immunoblotting using a nuclear membrane fraction. In immunostaining using MAb 21110, a punctuate nuclear rim staining pattern was observed. This MAb will be useful in immunoblotting and immunolocalization experiments in various cells and tissues, as well as further analyses of the biological function and cellular dynamics of this protein.

  • Nuclear-cytoplasmic shuttling of a RING-IBR protein RBCK1 and its functional interaction with nuclear body proteins. Reviewed

    Tatematsu K, Yoshimoto N, Koyanagi T, Tokunaga C, Tachibana T, Yoneda Y, Yoshida M, Okajima T, Tanizawa K, Kuroda S

    The Journal of biological chemistry   280 ( 24 )   22937 - 44   2005.06( ISSN:0021-9258

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M413476200

    PubMed

  • Nuclear-cytoplasmic shuttling of a RING-IBR protein RBCK1 and its functional interaction with nuclear body proteins Reviewed

    Kenji Tatematsu, Nobuo Yoshimoto, Tomoyoshi Koyanagi, Chiharu Tokunaga, Taro Tachibana, Yoshihiro Yoneda, Minoru Yoshida, Toshihide Okajima, Katsuyuki Tanizawai, Shun'ichi Kuroda

    Journal of Biological Chemistry   280 ( 24 )   22937 - 22944   2005.06( ISSN:0021-9258

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    The intracellular localization of a RING-IBR protein, RBCK1, possessing DNA binding and transcriptional activities, has been investigated. The endogenous RBCK1 was found in both the cytoplasm and nucleus. Particularly in the nucleus, it was localized in the granular structures, most likely nuclear bodies. In contrast, the over-expressed RBCK1 was detected exclusively in the cytoplasm. When the cells were treated with leptomycin B, the over-expressed RBCK1 accumulated in the nuclear bodies. These results suggest that RBCK1 possesses the signal sequences responsible for the nuclear-cytoplasmic translocation. Mutational analysis of RBCK1 has indicated that an N-terminal region containing Leu-142 and Leu-145 and a C-terminal one containing the RING-IBR domain serve as the nuclear export and localization signals, respectively. Thus, RBCK1 is a transcription factor dynamically shuttling between cytoplasm and nucleus. Furthermore, RBCK1 was found to interact with nuclear body proteins, CREB-binding protein (CBP), and promyelocytic leukemia protein (PML). Coexpression of RBCK1 with CBP significantly enhanced the transcriptional activity of RBCK1. Although PML per se showed no effect on the transcriptional activity of RBCK1, the CBP-enhanced activity was repressed by coexpression with PML, presumably through the interaction of PML and CBP. Taken together, our data demonstrate that RBCK1 is involved in transcriptional machinery in the nuclear bodies, and its transcriptional activity is regulated by nucleocytoplasmic shuttling. © 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

    DOI: 10.1074/jbc.M413476200

    PubMed

  • Extracellular signal-dependent nuclear import of STAT3 is mediated by various importin alphas. Reviewed

    Ushijima R, Sakaguchi N, Kano A, Maruyama A, Miyamoto Y, Sekimoto T, Yoneda Y, Ogino K, Tachibana T

    Biochemical and biophysical research communications   330 ( 3 )   880 - 6   2005.05( ISSN:0006-291X

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    DOI: 10.1016/j.bbrc.2005.03.063

    PubMed

  • Extracellular signal-dependent nuclear import of STAT3 is mediated by various importin alpha s Reviewed

    Ushijima R, Sakaguchi N, Kano A, Maruyama A, Miyamoto Y, Sekimoto T, Yoneda Y, Ogino K, Tachibana T

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   330 ( 3 )   880 - 886   2005.05( ISSN:0006-291X

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  • Extracellular signal-dependent nuclear import of STAT3 is mediated by various importin alpha s Reviewed

    Ushijima R, Sakaguchi N, Kano A, Maruyama A, Miyamoto Y, Sekimoto T, Yoneda Y, Ogino K, Tachibana T

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   330 ( 3 )   880 - 886   2005.05( ISSN:0006-291X

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  • Extracellular signal-dependent nuclear import of STAT3 is mediated by various importin alpha s Reviewed

    R Ushijima, N Sakaguchi, A Kano, A Maruyama, Y Miyamoto, T Sekimoto, Y Yoneda, K Ogino, T Tachibana

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   330 ( 3 )   880 - 886   2005.05( ISSN:0006-291X

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    The signal transducer and activator of transcription 3 (STAT3) is a transcription factor that is involved in a variety of biological functions. STAT3 is activated by cytokines and growth factors via the phosphorylation of a tyrosine residue, dimerization, and subsequent nuclear translocation. However, the mechanism of its nuclear translocation is unclear. A study of the cytokine-stimulated import of STAT3 into the nucleus is reported herein. An oncostatin M (OSM)-dependent nuclear import assay system was first established in living cells. Using this system, we demonstrated that the microinjection of the importin alpha 5/NPI-1 mutant, an anti-importin P antibody, and the RanQ69L mutant inhibited the nuclear import of STAT3. Second, we showed that tyrosine-phosphorylated STAT3 associates, not only with importin alpha 5/NPI-1 but also with other importin alpha s, as a result of OSM stimulation, as evidenced by a solution binding assay. These findings suggest that the extracellular signal-dependent nuclear transport of STAT3 is mediated by various importin alpha s, importin beta, and Ran. (c) 2005 Elsevier Inc. All rights reserved.

  • The RNA binding protein TLS is translocated to dendritic spines by mGluR5 activation and regulates spine morphology Reviewed

    Fujii R, Okabe S, Urushido T, Inoue K, Yoshimura A, Tachibana T, Nishikawa T, Hicks GG, Takumi T

    CURRENT BIOLOGY   15 ( 6 )   587 - 593   2005.03( ISSN:0960-9822

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    DOI: 10.1016/j.cub.2005.01.058

  • Enhanced nuclear import and transfection efficiency of plasmid DNA using streptavidin-fused importin-beta Reviewed

    Nagasaki T, Kawazu T, Tachibana T, Tamagaki S, Shinkai S

    JOURNAL OF CONTROLLED RELEASE   103 ( 1 )   199 - 207   2005.03( ISSN:0168-3659

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    DOI: 10.1016/j.jconrel.2004.11.024

  • Enhanced nuclear import and transfection efficiency of plasmid DNA using streptavidin-fused importin-beta

    T Nagasaki, T Kawazu, T Tachibana, S Tamagaki, S Shinkai

    JOURNAL OF CONTROLLED RELEASE   103 ( 1 )   199 - 207   2005.03( ISSN:0168-3659

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    in order to enhance the nuclear import of exogenous genes, novel plasmid DNA/importin-beta conjugates, which consist of a biotinylated plasmid. DNA and a recombinant streptavidin-fused importin-beta, were prepared. The spacer length between plasmid DNA and biotin and the number of introduced biotin were adjusted. The microinjection of plasmid DNA/importin-beta conjugates into the cytoplasm of NIH3T3 cells resulted in the nuclear localization of conjugates and the higher expression efficiency, compared to intact plasmid DNA alone. These results indicate that plasmid DNA/importin-beta conjugates would be an important tool to enhance the nuclear localization of exogenous DNA in non-viral gene delivery system. (c) 2004 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.jconrel.2004.11.024

  • The RNA binding protein TLS is translocated to dendritic spines by mGluR5 activation and regulates spine morphology Reviewed

    R Fujii, S Okabe, T Urushido, K Inoue, A Yoshimura, T Tachibana, T Nishikawa, GG Hicks, T Takumi

    CURRENT BIOLOGY   15 ( 6 )   587 - 593   2005.03( ISSN:0960-9822

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    Neuronal dendrites, together with dendritic spines, exhibit enormously diverse structure [1]. Selective targeting and local translation of mRNAs in dendritic spines have been implicated in synapse remodeling or synaptic plasticity [2, 3]. The mechanism of mRNA transport to the postsynaptic site is a fundamental question in local dendritic translation [4, 5]. TLS (translocated in liposarcoma), previously identified as a component of hnRNP complexes, unexpectedly showed somatodendritic localization in mature hippocampal pyramidal neurons. In the present study, TLS was translocated to dendrites and was recruited to dendrites not only via microtubules but also via actin filaments. In mature hippocampal pyramidal neurons, TLS accumulated in the spines at excitatory postsynapses upon mGIuR5 activation, which was accompanied by an increased RNA content in dendrites. Consistent with the in vitro studies, TLS-null hippocampal pyramidal neurons exhibited abnormal spine morphology and lower spine density. Our results indicate that TLS participates in mRNA sorting to the dendritic spines induced by mGIuR5 activation and regulates spine morphology to stabilize the synaptic structure.

    DOI: 10.1016/j.cub.2005.01.058

  • Lipase-catalyzed Preparation of Optically Active 1'-Acetoxychavicol Acetates and their Structure-Activity Relationships in Apoptotic Activity against Human Leukemia HL-60 Cells.

    Bioorg. Med. Chem.   14   1811 - 1818   2005

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  • Extracellular signal-dependent nuclear import of STAT3 is mediated by various importin alphas.

    Biochem Biophys Res Commun.   330   880 - 886   2005

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    Publishing type:Research paper (scientific journal)  

  • Nuclear-cytoplasmic shuttling of a RING-IBR protein RBCK1 and its functional interaction with nuclear body proteins.

    J Biol Chem.   280   22937 - 22944   2005

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  • The RNA Binding Protein TLS Is Translocated to Dendritic Spines by mGluR5 Activation and Regulates Spine Morphology.

    Curr. Biol.   15   587 - 593   2005

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  • Enhanced nuclear import and transfection efficiency of plasmid DNA using streptavidin-fused importin-beta.

    J. Control Release   103   199 - 207   2005

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    Publishing type:Research paper (scientific journal)  

  • Enhanced nuclear import and transfection efficiency of plasmid DNA using streptavidin-fused importin-beta. Reviewed

    J. Control Release   103   199 - 207   2005

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    Publishing type:Research paper (scientific journal)  

  • The RNA Binding Protein TLS Is Translocated to Dendritic Spines by mGluR5 Activation and Regulates Spine Morphology. Reviewed

    Curr. Biol.   15   587 - 593   2005

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    Publishing type:Research paper (scientific journal)  

  • Nuclear-cytoplasmic shuttling of a RING-IBR protein RBCK1 and its functional interaction with nuclear body proteins. Reviewed

    J Biol Chem.   280   22937 - 22944   2005

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    Publishing type:Research paper (scientific journal)  

  • Lipase-catalyzed Preparation of Optically Active 1'-Acetoxychavicol Acetates and their Structure-Activity Relationships in Apoptotic Activity against Human Leukemia HL-60 Cells. Reviewed

    Bioorg. Med. Chem.   14   1811 - 1818   2005

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    Publishing type:Research paper (scientific journal)  

  • Extracellular signal-dependent nuclear import of STAT3 is mediated by various importin alphas. Reviewed

    Biochem Biophys Res Commun.   330   880 - 886   2005

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    Publishing type:Research paper (scientific journal)  

  • Lipase-catalyzed Preparation of Optically Active 1'-Acetoxychavicol Acetates and their Structure-Activity Relationships in Apoptotic Activity against Human Leukemia HL-60 Cells. Reviewed

    Bioorg. Med. Chem.   14   1811 - 1818   2005

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    Publishing type:Research paper (scientific journal)  

  • Specific monoclonal antibody against nuclear import factor, importin alpha1/Rch1. Reviewed

    Kamikubo Y, Sakaguchi N, Shikata K, Furuta M, Miyamoto Y, Imamoto N, Yoneda Y, Ogino K, Tachibana T

    Hybridoma and hybridomics   23 ( 5 )   301 - 4   2004.10( ISSN:1536-8599

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/hyb.2004.23.301

    PubMed

  • Specific monoclonal antibody against nuclear import factor, importin alpha1/Rch1. Reviewed

    Kamikubo Y, Sakaguchi N, Shikata K, Furuta M, Miyamoto Y, Imamoto N, Yoneda Y, Ogino K, Tachibana T

    Hybridoma and hybridomics   23 ( 5 )   301 - 4   2004.10( ISSN:1536-8599

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/hyb.2004.23.301

    PubMed

  • Fatal hypothermia related vacuolation of hormone-producing cells in the anterior pituitary. Reviewed

    Ishikawa T, Miyaishi S, Tachibana T, Ishizu H, Zhu BL, Maeda H

    Legal medicine (Tokyo, Japan)   6 ( 3 )   157 - 63   2004.07( ISSN:1344-6223

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.legalmed.2004.05.004

    PubMed

  • (3Z)-2-Acetylamino-3-octadecen-1-ol as a potent apoptotic agent against HL-60 cells. Reviewed

    Niiro H, Azuma H, Tanago S, Matsumura K, Shikata K, Tachibana T, Ogino K

    Bioorganic & medicinal chemistry   12 ( 1 )   45 - 51   2004.01( ISSN:0968-0896

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    Publishing type:Research paper (scientific journal)  

    PubMed

  • (3Z)-2-Acetylamino-3-octadecen-1-ol as a potent apoptotic agent against HL-60 cells. Reviewed

    Niiro H, Azuma H, Tanago S, Matsumura K, Shikata K, Tachibana T, Ogino K

    Bioorganic & medicinal chemistry   12 ( 1 )   45 - 51   2004.01( ISSN:0968-0896

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bmc.2003.10.040

    PubMed

  • Specific monoclonal antibody against nuclear import factor, importin alpha1/Rch1.

    Hybridoma and Hybridomics   23   301 - 304   2004

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    Publishing type:Research paper (scientific journal)  

  • (3Z)-2-Acetylamino-3-octadecan-1-ol as a potent apoptotic agent againstHL-60 cells.

    Bioorg.Med.Chem.   12   45 - 51   2004

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    Publishing type:Research paper (scientific journal)  

  • Generation of a rat monoclonal antibody specific for importin alpha3/Qip1. Reviewed

    Sakaguchi N, Miyamoto Y, Yoneda Y, Ogino K, Tachibana T

    Hybridoma and hybridomics   22 ( 6 )   397 - 400   2003.12( ISSN:1536-8599

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/153685903771797110

    PubMed

  • Generation of a rat monoclonal antibody specific for importin alpha3/Qip1. Reviewed

    Sakaguchi N, Miyamoto Y, Yoneda Y, Ogino K, Tachibana T

    Hybridoma and hybridomics   22 ( 6 )   397 - 400   2003.12( ISSN:1536-8599

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/153685903771797110

    PubMed

  • Synthesis and potent antileukemic activities of N-lactylsphingosine and N-lactyldihydrosphingosine. Reviewed

    Azuma H, Takao R, Shikata K, Niiro H, Tachibana T, Ogino K

    Journal of medicinal chemistry   46 ( 16 )   3445 - 7   2003.07( ISSN:0022-2623

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/jm030125p

    PubMed

  • Synthesis and potent antileukemic activities of N-lactylsphingosine and N-lactyldihydrosphingosine. Reviewed

    Azuma H, Takao R, Shikata K, Niiro H, Tachibana T, Ogino K

    Journal of medicinal chemistry   46 ( 16 )   3445 - 7   2003.07( ISSN:0022-2623

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/jm030125p

    PubMed

  • Apoptotic activities of C2-ceramide and C2-dihydroceramide homologues against HL-60 cells. Reviewed

    Shikata K, Niiro H, Azuma H, Ogino K, Tachibana T

    Bioorganic & medicinal chemistry   11 ( 13 )   2723 - 8   2003.07( ISSN:0968-0896

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    Publishing type:Research paper (scientific journal)  

    PubMed

  • Apoptotic activities of C2-ceramide and C2-dihydroceramide homologues against HL-60 cells. Reviewed

    Shikata K, Niiro H, Azuma H, Ogino K, Tachibana T

    Bioorganic & medicinal chemistry   11 ( 13 )   2723 - 8   2003.07( ISSN:0968-0896

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/s0968-0896(03)00228-1

    PubMed

  • Total syntheses of symbioramide derivatives from l-serine and their antileukemic activities. Reviewed

    Azuma H, Takao R, Niiro H, Shikata K, Tamagaki S, Tachibana T, Ogino K

    The Journal of organic chemistry   68 ( 7 )   2790 - 7   2003.04( ISSN:0022-3263

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/jo0206824

    PubMed

  • Total syntheses of symbioramide derivatives from l-serine and their antileukemic activities. Reviewed

    Azuma H, Takao R, Niiro H, Shikata K, Tamagaki S, Tachibana T, Ogino K

    The Journal of organic chemistry   68 ( 7 )   2790 - 7   2003.04( ISSN:0022-3263

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/jo0206824

    PubMed

  • Can nuclear localization signals enhance nuclear localization of plasmid DNA? Reviewed

    Nagasaki T, Myohoji T, Tachibana T, Futaki S, Tamagaki S

    Bioconjugate chemistry   14 ( 2 )   282 - 6   2003.03( ISSN:1043-1802

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/bc025602h

    PubMed

  • Can nuclear localization signals enhance nuclear localization of plasmid DNA? Reviewed

    Nagasaki T, Myohoji T, Tachibana T, Futaki S, Tamagaki S

    Bioconjugate chemistry   14 ( 2 )   282 - 6   2003.03( ISSN:1043-1802

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/bc025602h

    PubMed

  • Synthesis of non-natural C2-homo-ceramide and its apoptotic activity against HL-60 cells. Reviewed

    Shikata K, Niiro H, Azuma H, Tachibana T, Ogino K

    Bioorganic & medicinal chemistry letters   13 ( 4 )   613 - 6   2003.02( ISSN:0960-894X

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    Publishing type:Research paper (scientific journal)  

    PubMed

  • Synthesis of non-natural C2-homo-ceramide and its apoptotic activity against HL-60 cells. Reviewed

    Shikata K, Niiro H, Azuma H, Tachibana T, Ogino K

    Bioorganic & medicinal chemistry letters   13 ( 4 )   613 - 6   2003.02( ISSN:0960-894X

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/s0960-894x(02)01026-0

    PubMed

  • Synthesis and potent antileukemic activities of N-Lactyl-sphingosine and N-Lactyl-dihydrosphingosine.

    J.Med.Chem.   46   3445 - 3447   2003

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    Publishing type:Research paper (scientific journal)  

  • Generation of a rat monoclonal antibody specific for importin alpha3/Qip1.

    Hybridoma and Hybridomics   22 ( 6 )   2003

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    Publishing type:Research paper (scientific journal)  

  • Can nuclear localization signals enhance nuclear localization of plasmid DNA?

    Bioconjug.Chem.   14   282 - 286   2003

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    Publishing type:Research paper (scientific journal)  

  • Apoptotic activities of C2-ceramide and C2-dihydroceramide homologues against HL-60 cells.

    Bioorg.Med.Chem.   11   2723 - 2728   2003

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    Publishing type:Research paper (scientific journal)  

  • Total syntheses of Symbioramide derivatives from L-serine and their antileukemic activities.

    J.Org.Chem.   68   2790 - 2797   2003

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    Publishing type:Research paper (scientific journal)  

  • Synthesis of non-natural C2-homo-ceramide and its apoptotic activity against HL-60 cells.

    Bioorg.Med.Chem.Lett.   13   613 - 616   2003

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    Publishing type:Research paper (scientific journal)  

  • A new technique to co-localise membrane proteins with Homer/vesl. Reviewed

    Hiroaki Y, Nishikawa K, Mitsuoka K, Tachibana T, Sobue K, Doi T, Fujiyoshi Y

    Biochemical and biophysical research communications   295 ( 3 )   756 - 65   2002.07( ISSN:0006-291X

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    Publishing type:Research paper (scientific journal)  

    PubMed

  • A new technique to co-localise membrane proteins with Homer/vesl.

    Biochem.Biophys.Res.Commun.   295   756 - 765   2002

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    Publishing type:Research paper (scientific journal)  

  • Synthesis of novel and non-natural ceramide analogues derived from L-glutamic acid.

    Tetrahedron   58   5803 - 5809   2002

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    Publishing type:Research paper (scientific journal)   Kind of work:Single Work  

  • Nuclear import of the U1A splicesome protein is mediated by importin a/b and ran in living mammalian cells.

    J.Biol.Chem.   276   16824 - 16832   2001

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    Publishing type:Research paper (scientific journal)  

  • The small GTP-binding protein TC10 promotes nerve elongation in neuronal cells, and its expression is induced during nerve regeneration in rats.

    J.Neurosci.   20   4138 - 4144   2000

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    Publishing type:Research paper (scientific journal)  

  • Characterization of the nuclear transport of a novel leucine-rich acidic nuclear protein-like protein.

    FEBS Lett.   468   171 - 175   2000

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    Publishing type:Research paper (scientific journal)  

  • *Recycling of importin-alpha from the nucleus is suppressed by loss of RCC1 function in living mammalian cells. (*CSF Award)

    Cell Struc. Func.   25   115 - 123   2000

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    Publishing type:Research paper (scientific journal)  

  • Necleocytoplasmic shuttling of the aryl hydrocarbon receptor.

    J.Biochem.   127   503 - 509   2000

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    Publishing type:Research paper (scientific journal)  

  • Involvement of unique leucine-zipper motif of PSD-Zip45(Homer 1c/vesl-1L) in group 1 metabotropic glutamate receptor clustering.

    96   13801 - 13806   1999

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    Publishing type:Research paper (scientific journal)  

  • b-Catenin can be transported into the nucleus in a Ran-unassisted manner.

    Mol.Biol.Cell   10   1119 - 1131   1999

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    Publishing type:Research paper (scientific journal)  

  • A monoclonal antibody to the C-terminal acidic portion of Ran inhibits both recycling of Ran and nuclear protein import in living cells.

    J.Cell Biol.   144   645 - 655   1999

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    Publishing type:Research paper (scientific journal)  

  • Up-regulation of nuclear protein import by nuclear localization signal sequences in living cells.

    FEBS Lett.   442   235 - 240   1999

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    Publishing type:Research paper (scientific journal)  

  • b-Subunit of nuclear pore-targeting complex (Importin-b can be exported from the nucleus in a Ran-unassisted manner.

    J.Biol.Chem.   274   3946 - 3952   1999

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    Publishing type:Research paper (scientific journal)  

  • Molecular Shape and ATP Binding Activity of Rat p50, a Putative Mammalian Homologue of RuvB DNA Helicase.

    J.Biochem.   125   487 - 497   1999

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    Publishing type:Research paper (scientific journal)  

  • Nuclear transport factor p10/NTF2 functions as a Ran-GDP dissociation inhibitor (Ran-GDI).

    Curr.Biol.   8   1339 - 1342   1998

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    Publishing type:Research paper (scientific journal)  

  • Nuclear localization and export signals of the human aryl hydrocarbon receptor.

    J.Biol.Chem.   273   2895 - 2904   1998

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    Publishing type:Research paper (scientific journal)  

  • Differentialmodes of nuclear localization signal (NLS) recognition by three distinct classes of NLS receptors.

    J.Biol.Chem.   272   26375 - 26381   1997

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    Publishing type:Research paper (scientific journal)  

  • Ran-unassisted nuclear migration of a 97-kD component of nuclear pore-targeting complex.

    J.Cell Biol.   139   841 - 849   1997

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    Publishing type:Research paper (scientific journal)  

  • A nuclear localization signal of human aryl hydrocarbon receptor nuclear translocator/hypoxia-inducible factor 1b is a novel bipartite type recognized by the two components of nuclear pore-targeting complex.

    J.Biol.Chem.   272   17640 - 17647   1997

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    Publishing type:Research paper (scientific journal)  

  • Essential role of active nuclear transport in apoptosis.

    Genes to Cells   2   55 - 64   1997

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    Publishing type:Research paper (scientific journal)  

  • Exogenously injected nuclear import factor p10/NTF2 inhibits signal-mediated nuclear import and export of proteins in living cells.

    FEBS Lett.   397   177 - 182   1996

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    Publishing type:Research paper (scientific journal)  

  • Subcellular distribution and phosphorylation of the nuclear localization signal binding protein.

    Exp.Cell Res.   222   385 - 394   1996

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    Publishing type:Research paper (scientific journal)  

  • Interferon-g-dependent nuclear import of Stat1 is mediated by the GTPase activity of Ran/TC4.

    J.Biol.Chem.   271   31017 - 31020   1996

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    Publishing type:Research paper (scientific journal)  

  • Ran and nuclear protein import

    14 ( 2 )   260 - 263   1996

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    Publishing type:Research paper (scientific journal)  

  • Induction of neurite outgrowth by MAP kinase in PC12 cells.

    Oncogene   11   239 - 244   1995

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    Publishing type:Research paper (scientific journal)  

  • In vivo evidence for involvement of a 58kDa component of nuclear pore-targeting complex in nuclear protein import.

    EMBO J.   14   3617 - 3626   1995

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    Publishing type:Research paper (scientific journal)  

  • Nuclear pore-targeting complex binds to nuclear pore after association with a karyophile.

    FEBS Lett.   368   415 - 419   1995

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    Publishing type:Research paper (scientific journal)  

  • A karyophilic protein forms a stable complex with cytoplasmic components prior to nuclear pore binding.

    J.Biol.Chem.   270   8559 - 8565   1995

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    Publishing type:Research paper (scientific journal)  

  • Interconversion of bacteriochlorophyll c aggregates in solid films upon organic vapor treatment.

    Photochem. Photobiol.   62   496 - 501   1995

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    Publishing type:Research paper (scientific journal)  

  • Loss of RCC1 leads to the suppression of nuclear protein import in living cells.

    J.Biol.Chem.   269   24542 - 24545   1994

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    Publishing type:Research paper (scientific journal)  

  • Structure and interconversion of bacteriochlorophyll c aggregates in solid films.

    Reseach in Photosynthesis   1   85 - 88   1992

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    Publishing type:Research paper (scientific journal)  

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MISC

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Presentations

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Outline of collaborative research (seeds)

  • Production of monoclonal antibodies

    2001-

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    Request for collaborative research:The private sector, such as other institutions

    Type of research exchange:Technical consultation, Contract research, Joint research

Grant-in-Aid for Scientific Research

  • 特異的抗体を用いた転移性がん細胞表面分子の同定と創薬応用

    Grant-in-Aid for Scientific Research(C)  2022.04

  • 生活習慣病モデルメダカを用いた歯周病合併症の予防抑制効果を示す食品由来物質の探索

    Grant-in-Aid for Scientific Research(B)  2022

  • 二重特異性抗体を用いたBNCTホウ素デリバリーシステムの開発

    Grant-in-Aid for Scientific Research(B)  2019

  • 世界的流行を示す腸管毒素原性大腸菌O169の新規接着因子による人獣共通感染の検証

    Grant-in-Aid for Scientific Research(B)  2019

  • 1細胞ラビット組換え抗体作製技術を用いた新規がん幹細胞表面マーカー分子の探索

    Grant-in-Aid for Scientific Research(C)  2019

Other subsidies, etc.

  • HBV感染を阻害するモノクローナル抗体の作製

    国立研究開発法人日本医療研究開発機構(AMED)  肝炎等克服実用化研究事業(B型肝炎創薬実用化等研究事業)  2023

  • HBV感染を阻害するモノクローナル抗体の作製

    国立研究開発法人日本医療研究開発機構(AMED)  肝炎等克服実用化研究事業(B型肝炎創薬実用化等研究事業)  2022

  • HBVの感染を阻害するモノクローナル抗体の開発

    国立研究開発法人日本医療研究開発機構(AMED)  肝炎等克服実用化研究事業(B型肝炎創薬実用化等研究事業)  2021

  • HBVの感染を阻害するモノクローナル抗体の開発

    国立研究開発法人日本医療研究開発機構(AMED)  肝炎等克服実用化研究事業(B型肝炎創薬実用化等研究事業)  2020

  • HBVの感染を阻害するモノクローナル抗体の開発

    国立研究開発法人日本医療研究開発機構(AMED)  肝炎等克服実用化研究事業(B型肝炎創薬実用化等研究事業)  2019

  • HBVの感染を阻害するモノクローナル抗体の開発

    国立研究開発法人日本医療研究開発機構(AMED)  肝炎等克服実用化研究事業(B型肝炎創薬実用化等研究事業)  2018

  • HBVの感染を阻害するモノクローナル抗体の開発

    国立研究開発法人日本医療研究開発機構(AMED)  肝炎等克服実用化研究事業(B型肝炎創薬実用化等研究事業)  2017

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Acceptance of Researcher

  • 2022  Number of researchers:1

  • 2021  Number of researchers:1

  • 2020  Number of researchers:1

  • 2019  Number of researchers:1

Charge of on-campus class subject

  • 物質化学生命系特別演習

    2022   Intensive lecture   Graduate school

  • 創薬生命工学特論

    2022   Weekly class   Graduate school

  • 物質化学生命系特別演習第1

    2022   Intensive lecture   Graduate school

  • 卒業研究Ⅰ

    2022   Intensive lecture   Undergraduate

  • 化学バイオ工学特論Ⅰ

    2022   Intensive lecture   Undergraduate

  • 化学バイオ工学概論

    2022   Intensive lecture   Undergraduate

  • 物質化学生命系特別研究 (化学バイオ工学分野)

    2022   Intensive lecture   Graduate school

  • 物質化学生命系特別演習第2 (化学バイオ工学分野)

    2022   Intensive lecture   Graduate school

  • 特別演習(化学生物系2回生)

    2022   Intensive lecture   Graduate school

  • 前期特別研究(化学生物系)

    2022   Intensive lecture   Graduate school

  • 後期特別研究(化学生物系)

    2022   Intensive lecture   Graduate school

  • ゼミナール(化学生物系)

    2022   Intensive lecture   Graduate school

  • 卒業研究

    2022   Intensive lecture   Undergraduate

  • バイオテクノロジー概論

    2022   Weekly class   Undergraduate

  • バイオ工学実験

    2022   Weekly class   Undergraduate

  • バイオ英語演習

    2022   Weekly class   Undergraduate

  • 化学バイオ工学特論

    2022   Intensive lecture   Undergraduate

  • 化学バイオ工学特論

    2018     Undergraduate

  • 創薬分子工学特論

    2018     Graduate school

  • バイオテクノロジー概論

    2018     Undergraduate

  • 細胞生物学

    2018     Undergraduate

  • バイオ工学実験

    2018     Undergraduate

  • 化学バイオ工学概論

    2018     Undergraduate

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Charge of off-campus class subject

  • 保健学研究共通特講Ⅰ・V

    2022.04
    Institution:Kobe University

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    Level:Postgraduate 

  • 保健学研究共通特講Ⅰ・V

    2021.04
    Institution:Kobe University

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    Level:Postgraduate 

  • 保健学研究共通特講Ⅰ・V

    2020.04
    Institution:Kobe University

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    Level:Postgraduate 

  • 医薬品科学

  • 生物科学入門

  • 細胞情報学特論

  • 蛋白質科学

  • 超分子化学

  • 技術と生命

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Faculty development activities

  • FD活動への貢献  2017

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    学内各FD集会参加

Social Activities

  • 抗体医薬 -21世紀の魔法の弾丸-

    Role(s): Lecturer

    大阪市立大学文化交流センター  公開講座「温故知新」  大阪市立大学文化交流センター  2017.11

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    Audience: General public

    Type:Lecture

    Number of participants:80(人)

    バイオテクノロジーの進歩により、これまでとは全く異なるタイプの医薬品が登場しました。それが抗体医薬です。関節リウマチやガンなどで高い治療効果をあげている抗体医薬について、基礎からわかりやすく解説します。

Foreigner acceptance

  • 2019

    International Students :1

  • 2018

    International Students :1

  • 2017

    International Students :1

Job title

  • Manager within the university

    学長特別補佐  2021.04 - 2022.03

  • Job title within the department

    専攻長  2021.04 - 2022.03

Other

  • Job Career

    2017.10 - Now

  • Job Career

    2007.04 - 2017.09

  • Job Career

    2001.04 - 2007.03