2024/04/08 更新

写真a

タチバナ タロウ
立花 太郎
TACHIBANA Taro
担当
大学院工学研究科 物質化学生命系専攻 教授
工学部 化学バイオ工学科
職名
教授
所属
工学研究院
ホームページ
所属キャンパス
杉本キャンパス

担当・職階

  • 大学院工学研究科 物質化学生命系専攻 

    教授  2022年04月 - 継続中

  • 工学部 化学バイオ工学科 

    教授  2022年04月 - 継続中

取得学位

  • 博士(医学) ( 大阪大学 )

研究分野

  • ライフサイエンス / 細胞生物学  / 分子細胞生物学

研究キーワード

  • 抗体医薬品

  • モノクローナル抗体

研究概要

  • 現在、モノクローナル抗体はバイオ分野のツールとしてだけでなく、検査薬や治療薬(抗体医薬品)として活用され、私たちの暮らしに欠かせないものとなっています。抗体医薬品は癌やリウマチなどの疾患の治療に目覚ましい成果を上げ、今や世界の医薬品売上ランキングの上位を占めるまでに至っています。今後さらに抗体医薬品への期待は高まるばかりですが、高特異性・高親和性の抗体作製は非常に難易度が高く、新しい高性能抗体作製技術の開発が望まれています。優れた技術の確立は抗体医薬品新薬の開発に直結します。つまり、抗体作製技術の開発研究は、創薬研究そのものと言えます。これまでのモノクローナル抗体の作製にはマウスやラットが用いられてきました。創薬生命工学研究室では、マウスやラットだけでなく、ウサギやウシさらにはヒトなど、より優れた免疫システムを持つ動物を利用し、ゲノム編集技術など高度な生命工学手法を駆使し、高性能な抗体を高効率に作製する方法の開発に取り組んでいます。さらに、国内外の研究者と積極的に共同研究を行い、特定の幹細胞や分化細胞特異的抗原など、様々なターゲットに対する抗体作製とそれら抗体のユニークな利用法についての応用研究を行っています。

研究歴

  • 新規モノクローナル抗体作製法の開発

    モノクローナル抗体  個人研究

    1993年04月 

所属学協会

  • 日本細胞生物学会

      国内

  • 日本抗体学会

    2023年 - 継続中   国内

受賞歴

  • 日本細胞生物学会論文賞

    2001  

     詳細を見る

    受賞国:日本国

  • 山村賞

    1995  

     詳細を見る

    受賞国:日本国

職務経歴(学外)

  • パンクセラピー株式会社   代表取締役

    2022年02月 - 継続中

  • 株式会社細胞工学研究所   代表取締役

    2010年02月 - 継続中

  • 米国マサチューセッツ大学医学部   客員研究員

    2006年04月 - 2007年03月

  • 大阪大学大学院医学研究科・助手   -

    1998年 - 2001年

学歴

  • 大阪大学   医学研究科   生理系   博士課程   卒業・修了

    - 1996年

  • 大阪府立大学   工学部   応用化学科     卒業・修了

    - 1990年

論文

  • Production of a Monoclonal Antibody for Histone H2b Isoform H2b3b. 査読

    Egashira S, Tachibana T, Nakamura M, Ohkawa Y, Harada A

    Monoclonal antibodies in immunodiagnosis and immunotherapy   2024年03月

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/mab.2023.0025

    PubMed

  • Vero cell-adapted SARS-CoV-2 strain shows increased viral growth through furin-mediated efficient spike cleavage. 査読

    Minami S, Kotaki T, Sakai Y, Okamura S, Torii S, Ono C, Motooka D, Hamajima R, Nouda R, Nurdin JA, Yamasaki M, Kanai Y, Ebina H, Maeda Y, Okamoto T, Tachibana T, Matsuura Y, Kobayashi T

    Microbiology spectrum   e0285923   2024年02月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1128/spectrum.02859-23

    PubMed

  • Development of a high-affinity anti-bovine PD-1 rabbit-bovine chimeric antibody using an efficient selection and large production system. 査読

    Okagawa T, Konnai S, Goto S, Sajiki Y, Ganbaatar O, Watari K, Nakamura H, Wang CX, Tachibana T, Kato Y, Kameda Y, Kohara J, Terasaki N, Kubota M, Takeda A, Takahashi H, Suzuki Y, Maekawa N, Murata S, Ohashi K

    Veterinary research   54 ( 1 )   82   2023年09月( ISSN:0928-4249

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1186/s13567-023-01213-6

    PubMed

  • Generation of Rat Monoclonal Antibody for Human Nucleolin. 査読

    Nishino Y, Homma T, Ihara KI, Fujii J, Tachibana T, Yokoyama C

    Monoclonal antibodies in immunodiagnosis and immunotherapy   42 ( 4 )   145 - 149   2023年08月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/mab.2023.0008

    PubMed

  • Effective SARS-CoV-2 replication of monolayers of intestinal epithelial cells differentiated from human induced pluripotent stem cells. 査読

    Minami S, Matsumoto N, Omori H, Nakamura Y, Tamiya S, Nouda R, Nurdin JA, Yamasaki M, Kotaki T, Kanai Y, Okamoto T, Tachibana T, Ushijima H, Kobayashi T, Sato S

    Scientific reports   13 ( 1 )   11610   2023年07月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/s41598-023-38548-1

    PubMed

  • Generation of Rat Monoclonal Antibodies Against Human Epidermal Growth Factor Receptor 2. 査読

    Yamamoto H, Nakanishi T, Ihara KI, Tachibana T, Yokoyama C

    Monoclonal antibodies in immunodiagnosis and immunotherapy   42 ( 2 )   59 - 64   2023年04月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/mab.2022.0042

    PubMed

  • Generation of Rat Monoclonal Antibody for Mouse Nucleolin by Immunization of Ferroptosis-Induced Hepa 1-6 Cells. 査読

    Yokoyama C, Kobayashi S, Harada Y, Nishino Y, Fujii J, Tachibana T

    Monoclonal antibodies in immunodiagnosis and immunotherapy   41 ( 5 )   255 - 259   2022年10月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/mab.2022.0005

    PubMed

  • SARS-CoV-2 ORF6 disrupts nucleocytoplasmic trafficking to advance viral replication. 査読

    Miyamoto Y, Itoh Y, Suzuki T, Tanaka T, Sakai Y, Koido M, Hata C, Wang CX, Otani M, Moriishi K, Tachibana T, Kamatani Y, Yoneda Y, Okamoto T, Oka M

    Communications biology   5 ( 1 )   483   2022年05月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/s42003-022-03427-4

    PubMed

  • Establishment of monoclonal antibodies broadly neutralize infection of hepatitis B virus. 査読

    Zhang H, Itoh Y, Suzuki T, Ihara KI, Tanaka T, Haga S, Enatsu H, Yumiya M, Kimura M, Takada A, Itoh D, Shibazaki Y, Nakao S, Yoshio S, Miyakawa K, Miyamoto Y, Sasaki H, Kajita T, Sugiyama M, Mizokami M, Tachibana T, Ryo A, Moriishi K, Miyoshi E, Kanto T, Okamoto T, Matsuura Y

    Microbiology and immunology   66 ( 4 )   179 - 192   2022年04月( ISSN:0385-5600

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/1348-0421.12964

    PubMed

  • Unusual nucleosome formation and transcriptome influence by the histone H3mm18 variant. 査読

    Hirai S, Tomimatsu K, Miyawaki-Kuwakado A, Takizawa Y, Komatsu T, Tachibana T, Fukushima Y, Takeda Y, Negishi L, Kujirai T, Koyama M, Ohkawa Y, Kurumizaka H

    Nucleic acids research   50 ( 1 )   72 - 91   2022年01月( ISSN:0305-1048

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/nar/gkab1137

    PubMed

  • Septin-microtubule association via a motif unique to isoform 1 of septin 9 tunes stress fibers. 査読

    Kuzmić M, Castro Linares G, Leischner Fialová J, Iv F, Salaün D, Llewellyn A, Gomes M, Belhabib M, Liu Y, Asano K, Rodrigues M, Isnardon D, Tachibana T, Koenderink GH, Badache A, Mavrakis M, Verdier-Pinard P

    Journal of cell science   135 ( 1 )   2022年01月( ISSN:0021-9533

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1242/jcs.258850

    PubMed

  • Gα(q) modulates the energy metabolism of osteoclasts. 査読

    Chakraborty S, Handrick B, Yu D, Bode KA, Hafner A, Schenz J, Schaack D, Uhle F, Tachibana T, Kamitani S, Vogl T, Kubatzky KF

    Frontiers in cellular and infection microbiology   12   1016299 - 1016299   2022年

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    DOI: 10.3389/fcimb.2022.1016299

    PubMed

  • Canopy Homolog 2 as a Novel Molecular Target in Hepatocarcinogenesis. 査読

    Kakehashi A, Suzuki S, Shiota M, Raymo N, Gi M, Tachibana T, Stefanov V, Wanibuchi H

    Cancers   13 ( 14 )   2021年07月( ISSN:2072-6694

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.3390/cancers13143613

    PubMed

  • Wound, pressure ulcer and burn guidelines – 6: Guidelines for the management of burns, second edition 査読

    Yoshino Y.

    Journal of Dermatology   47 ( 11 )   1207 - 1235   2020年11月( ISSN:03852407

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/1346-8138.15335

  • Detection of Rhizopus-specific antigen in human and murine serum and bronchoalveolar lavage. 査読

    Shibata W, Niki M, Sato K, Fujimoto H, Yamada K, Watanabe T, Miyazaki Y, Asai K, Obata Y, Tachibana T, Kawaguchi T, Kaneko Y, Kakeya H

    Medical mycology   58 ( 7 )   958 - 964   2020年10月( ISSN:1369-3786

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/mmy/myaa001

    PubMed

  • Wound, pressure ulcer and burn guidelines – 4: Guidelines for the management of connective tissue disease/vasculitis-associated skin ulcers 査読

    Fujimoto M.

    Journal of Dermatology   47 ( 10 )   1071 - 1109   2020年10月( ISSN:03852407

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/1346-8138.15186

  • Outcome of Allogeneic Hematopoietic Stem Cell Transplantation in Patients with Myelodysplastic/Myeloproliferative Neoplasms-Unclassifiable: A Retrospective Nationwide Study of the Japan Society for Hematopoietic Cell Transplantation 査読

    Kurosawa S.

    Biology of Blood and Marrow Transplantation   26 ( 9 )   1607 - 1611   2020年09月( ISSN:10838791

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.bbmt.2020.05.013

  • Wound, pressure ulcer and burn guidelines – 1: Guidelines for wounds in general, second edition 査読

    Hasegawa M.

    Journal of Dermatology   47 ( 8 )   807 - 833   2020年08月( ISSN:03852407

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/1346-8138.15401

  • Generation of the Rat Monoclonal Antibody Against the Extracellular Domain of Human CD63 by DNA Immunization. 査読

    Xu L, Ihara KI, Yoshimura S, Konno D, Tachibana A, Nakanishi T, Tachibana T

    Monoclonal antibodies in immunodiagnosis and immunotherapy   39 ( 3 )   74 - 76   2020年06月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/mab.2020.0007

    PubMed

  • A straightforward approach to antibodies recognising cancer specific glycopeptidic neoepitopes. 査読

    Wakui H, Tanaka Y, Ose T, Matsumoto I, Kato K, Min Y, Tachibana T, Sato M, Naruchi K, Martin FG, Hinou H, Nishimura SI

    Chemical science   11 ( 19 )   4999 - 5006   2020年04月( ISSN:2041-6520

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1039/d0sc00317d

    PubMed

  • Preparation and Characterization of a Novel Bispecific Antibody That Targets Her2 and BSH for Boron Neutron Capture Therapy

    立花 太郎, 中西 猛, 真田 悠生

    KURNS Progress Report 2019   -   2020年

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    掲載種別:研究論文(学術雑誌)  

  • PACT/PRKRA and p53 regulate transcriptional activity of DMRT1. 査読

    Fujitani K, Otomo A, Nagayama Y, Tachibana T, Kato R, Kawashima Y, Kodera Y, Kato T, Takada S, Tamura K, Takamatsu N, Ito M

    Genetics and molecular biology   43 ( 2 )   e20190017   2020年( ISSN:1415-4757

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1590/1678-4685-GMB-2019-0017

    PubMed

  • Carbohydrate 3′-sialyllactose as a novel target for theranostics in pancreatic ductal adenocarcinoma 査読

    Higashi K.

    Tumor Biology   42 ( 10 )   2020年( ISSN:10104283

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1177/1010428320965279

  • Hematopoietic Stem Cell Transplantation From a Related Donor with Human Leukocyte Antigen 1-Antigen Mismatch in the Graft-Versus-Host Direction Using Low-dose Anti-thymocyte Globulin 査読

    Kanda J.

    Cell Transplantation   29   2020年( ISSN:09636897

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1177/0963689720976567

  • The effect of melphalan dose and total body irradiation as reduced-intensity conditioning for acute lymphoblastic leukemia patients undergoing allogeneic stem cell transplantation 査読

    Harada K.

    Leukemia and Lymphoma   60 ( 14 )   3521 - 3528   2019年12月( ISSN:10428194

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1080/10428194.2019.1636986

  • Crumbs3 is a critical factor that regulates invasion and metastasis of colon adenocarcinoma via the specific interaction with FGFR1. 査読

    Iioka H, Saito K, Sakaguchi M, Tachibana T, Homma K, Kondo E

    International journal of cancer   145 ( 10 )   2740 - 2753   2019年11月( ISSN:0020-7136

     詳細を見る

    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    DOI: 10.1002/ijc.32336

    PubMed

  • Chromatin-bound CRM1 recruits SET-Nup214 and NPM1c onto <i>HOX</i> clusters causing aberrant <i>HOX</i> expression in leukemia cells. 査読

    Oka M, Mura S, Otani M, Miyamoto Y, Nogami J, Maehara K, Harada A, Tachibana T, Yoneda Y, Ohkawa Y

    eLife   8   2019年11月

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    DOI: 10.7554/eLife.46667

    PubMed

  • Different impact of BCR-ABL transcripts on allogeneic hematopoietic cell transplantation from different graft sources for Ph + ALL with minimal residual disease. 査読

    Nishiwaki S, Mizuta S, Ohashi K, Fukuda T, Uchida N, Tachibana T, Onizuka M, Ozawa Y, Onishi Y, Takahashi S, Eto T, Nakamae H, Tanaka J, Ichinohe T, Atsuta Y, Kako S, Adult Acute Lymphoblastic Leukemia Working Group of the Japan Society for Hematopoietic Cell Transplantation

    American journal of hematology   94 ( 11 )   E301 - E305   2019年11月( ISSN:0361-8609

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1002/ajh.25612

    PubMed

  • GPAT2 is required for piRNA biogenesis, transposon silencing, and maintenance of spermatogonia in mice†. 査読

    Shiromoto Y, Kuramochi-Miyagawa S, Nagamori I, Chuma S, Arakawa T, Nishimura T, Hasuwa H, Tachibana T, Ikawa M, Nakano T

    Biology of reproduction   101 ( 1 )   248 - 256   2019年07月( ISSN:0006-3363

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    DOI: 10.1093/biolre/ioz056

    PubMed

  • GPAT2 is required for piRNA biogenesis, transposon silencing, and maintenance of spermatogonia in mice. 査読

    Shiromoto Y, Kuramochi-Miyagawa S, Nagamori I, Chuma S, Arakawa T, Nishimura T, Hasuwa H, Tachibana T, Ikawa M, Nakano T

    Biology of reproduction   101 ( 1 )   248 - 256   2019年04月( ISSN:0006-3363

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/biolre/ioz056

    PubMed

  • Crumbs3 is a critical factor that regulates invasion and metastasis of colon adenocarcinoma via the specific interaction with FGFR1. 査読

    Iioka H, Saito K, Sakaguchi M, Tachibana T, Homma K, Kondo E

    International journal of cancer   145 ( 10 )   2740 - 2753   2019年04月( ISSN:0020-7136

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1002/ijc.32336

    PubMed

  • The <i>STK35</i> locus contributes to normal gametogenesis and encodes a lncRNA responsive to oxidative stress. 査読

    Miyamoto Y, Whiley PAF, Goh HY, Wong C, Higgins G, Tachibana T, McMenamin PG, Mayne L, Loveland KL

    Biology open   7 ( 8 )   2018年08月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1242/bio.032631

    PubMed

  • The <i>STK35</i> locus contributes to normal gametogenesis and encodes a lncRNA responsive to oxidative stress. 査読

    Miyamoto Y, Whiley PAF, Goh HY, Wong C, Higgins G, Tachibana T, McMenamin PG, Mayne L, Loveland KL

    Biology open   7 ( 8 )   2018年08月( ISSN:2046-6390

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1242/bio.032631

    PubMed

  • EB1-binding-myomegalin protein complex promotes centrosomal microtubules functions 査読

    Bouguenina Habib, Salaun Daniele, Mangon Aurelie, Muller Leslie, Baudelet Emilie, Camoin Luc, Tachibana Taro, Cianferani Sarah, Audebert Stephane, Verdier-Pinard Pascal, Badache Ali

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   114 ( 50 )   E10687 - E10696   2017年12月( ISSN:0027-8424

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1073/pnas.1705682114

  • EB1-binding-myomegalin protein complex promotes centrosomal microtubules functions. 査読

    Bouguenina H, Salaun D, Mangon A, Muller L, Baudelet E, Camoin L, Tachibana T, Cianférani S, Audebert S, Verdier-Pinard P, Badache A

    Proceedings of the National Academy of Sciences of the United States of America   114 ( 50 )   E10687 - E10696   2017年12月( ISSN:0027-8424

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1073/pnas.1705682114

    PubMed

  • A statistical image analysis framework for pore-free islands derived from heterogeneity distribution of nuclear pore complexes 査読

    Mimura Yasuhiro, Takemoto Satoko, Tachibana Taro, Ogawa Yutaka, Nishimura Masaomi, Yokota Hideo, Imamoto Naoko

    SCIENTIFIC REPORTS   7   2017年11月( ISSN:2045-2322

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/s41598-017-16386-2

  • A statistical image analysis framework for pore-free islands derived from heterogeneity distribution of nuclear pore complexes. 査読

    Mimura Y, Takemoto S, Tachibana T, Ogawa Y, Nishimura M, Yokota H, Imamoto N

    Scientific reports   7 ( 1 )   16315   2017年11月( ISSN:2045-2322

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/s41598-017-16386-2

    PubMed

  • Crystal Structure and Characterization of Novel Human Histone H3 Variants, H3.6, H3.7, and H3.8 査読

    Taguchi Hiroyuki, Xie Yan, Horikoshi Naoki, Maehara Kazumitsu, Harada Akihito, Nogami Jumpei, Sato Koichi, Arimura Yasuhiro, Osakabe Akihisa, Kujirai Tomoya, Iwasaki Takeshi, Semba Yuichiro, Tachibana Taro, Kimura Hiroshi, Ohkawa Yasuyuki, Kurumizaka Hitoshi

    BIOCHEMISTRY   56 ( 16 )   2184 - 2196   2017年04月( ISSN:0006-2960

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/acs.biochem.6b01098

  • Crystal Structure and Characterization of Novel Human Histone H3 Variants, H3.6, H3.7, and H3.8. 査読

    Taguchi H, Xie Y, Horikoshi N, Maehara K, Harada A, Nogami J, Sato K, Arimura Y, Osakabe A, Kujirai T, Iwasaki T, Semba Y, Tachibana T, Kimura H, Ohkawa Y, Kurumizaka H

    Biochemistry   56 ( 16 )   2184 - 2196   2017年04月( ISSN:0006-2960

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/acs.biochem.6b01098

    PubMed

  • Septin 9_i2 is downregulated in tumors, impairs cancer cell migration and alters subnuclear actin filaments 査読

    Verdier-Pinard P., Salaun D., Bouguenina H., Shimada S., Pophillat M., Audebert S., Agavnian E., Coslet S., Charafe-Jauffret E., Tachibana T., Badache A.

    SCIENTIFIC REPORTS   7   2017年03月( ISSN:2045-2322

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/srep44976

  • Flocculation of Real Sewage Sludge Using Poly-gamma-glutamic Acid Produced by <it>Bacillus</it> sp Isolated from Soil 査読

    Liu Tao, Yamashita Kyouhei, Fukumoto Yoshihiro, Tachibana Taro, Azuma Masayuki

    公益社団法人 化学工学会 JOURNAL OF CHEMICAL ENGINEERING OF JAPAN   50 ( 3 )   201 - 206   2017年03月( ISSN:0021-9592

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    掲載種別:研究論文(学術雑誌)  

    <p>To promote the use of sewage sludge on farmland, a flocculant compatible with agricultural application is required. In general, an inorganic flocculant such as aluminium sulfate is used at large sewage disposal plants. However, there is concern that aluminium accumulates when it is returned to farmland, and therefore a biodegradable rather than an inorganic flocculant is required. To obtain biodegradable flocculants produced by microorganisms, the latter were isolated from soils and sewage sludges, and the flocculation activity of the culture broths was evaluated. Here, real sewage sludge was used for the evaluation because practical use was regarded as an important consideration. As a result, two strains, V13 and RK14, were selected and identified as <i>Bacillus megaterium</i> and <i>Bacillus licheniformis</i>, respectively. It was also found that flocculation was caused by poly-γ-glutamic acid (γ-PGA). γ-PGA from RK14 cells had considerably higher molecular weight than commercial γ-PGA, and the enantiomeric composition differed from that of γ-PGA from other <i>B. licheniformis</i>. To obtain mutants with high γ-PGA productivity, RK14 cells were treated with ethyl methanesulfonate, and a mutant (RK14-46) was selected. The culture medium suitable for γ-PGA production by RK14-46 cells was totally different from that by RK-14 cells. The γ-PGA production reached 21.1 g/L when RK14-46 cells were cultured in the optimum semi-synthetic medium at 30°C for 2 d. Furthermore, the combination of γ-PGA obtained from RK14-46 cells and chitosan, a cationic flocculant, was very effective for flocculation of real sewage sludge.</p>

    DOI: 10.1252/jcej.16we158

    CiNii Article

  • Septin 9_i2 is downregulated in tumors, impairs cancer cell migration and alters subnuclear actin filaments. 査読

    Verdier-Pinard P, Salaun D, Bouguenina H, Shimada S, Pophillat M, Audebert S, Agavnian E, Coslet S, Charafe-Jauffret E, Tachibana T, Badache A

    Scientific reports   7   44976   2017年03月( ISSN:2045-2322

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/srep44976

    PubMed

  • Application of Monoclonal Antibodies Against Mouse Dermokine. 査読

    Higashi K, Wang CX, Yokoyama C, Yamada K, Saito K, Tachibana T

    Monoclonal antibodies in immunodiagnosis and immunotherapy   36 ( 1 )   15 - 19   2017年02月( ISSN:2167-9436

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/mab.2016.0051

    PubMed

  • Application of Monoclonal Antibodies Against Mouse Dermokine. 査読

    Higashi K, Wang CX, Yokoyama C, Yamada K, Saito K, Tachibana T

    Monoclonal antibodies in immunodiagnosis and immunotherapy   36 ( 1 )   15 - 19   2017年02月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/mab.2016.0051

    PubMed

  • Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis 査読

    Ueda Jun, Harada Akihito, Urahama Takashi, Machida Shinichi, Maehara Kazumitsu, Hada Masashi, Makino Yoshinori, Nogami Jumpei, Horikoshi Naoki, Osakabe Akihisa, Taguchi Hiroyuki, Tanaka Hiroki, Tachiwana Hiroaki, Yao Tatsuma, Yamada Minami, Iwamoto Takashi, Isotani Ayako, Ikawa Masahito, Tachibana Taro, Okada Yuki, Kimura Hiroshi, Ohkawa Yasuyuki, Kurumizaka Hitoshi, Yamagata Kazuo

    CELL REPORTS   18 ( 3 )   593 - 600   2017年01月( ISSN:2211-1247

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.celrep.2016.12.065

  • Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis. 査読

    Ueda J, Harada A, Urahama T, Machida S, Maehara K, Hada M, Makino Y, Nogami J, Horikoshi N, Osakabe A, Taguchi H, Tanaka H, Tachiwana H, Yao T, Yamada M, Iwamoto T, Isotani A, Ikawa M, Tachibana T, Okada Y, Kimura H, Ohkawa Y, Kurumizaka H, Yamagata K

    Cell reports   18 ( 3 )   593 - 600   2017年01月( ISSN:2211-1247

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.celrep.2016.12.065

    PubMed

  • Flocculation of Real Sewage Sludge Using Poly-γ-glutamic Acid Produced by Bacillus sp. Isolated from Soil

    50 ( 3 )   201 - 206   2017年( ISSN:00219592

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1252/jcej.16we158

    CiNii Article

  • ARHGEF10 directs the localization of Rab8 to Rab6-positive executive vesicles 査読

    Shibata Satoshi, Kawanai Tsubasa, Hara Takayuki, Yamamoto Asuka, Chaya Taro, Tokuhara Yasunori, Tsuji Chinami, Sakai Manabu, Tachibana Taro, Inagaki Shinobu

    JOURNAL OF CELL SCIENCE   129 ( 19 )   3620 - 3634   2016年10月( ISSN:0021-9533

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1242/jcs.186817

  • ARHGEF10 directs the localization of Rab8 to Rab6-positive executive vesicles. 査読

    Shibata S, Kawanai T, Hara T, Yamamoto A, Chaya T, Tokuhara Y, Tsuji C, Sakai M, Tachibana T, Inagaki S

    Journal of cell science   129 ( 19 )   3620 - 3634   2016年10月( ISSN:0021-9533

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1242/jcs.186817

    PubMed

  • The wound/burn guidelines - 6: Guidelines for the management of burns. 査読

    Yoshino Y, Ohtsuka M, Kawaguchi M, Sakai K, Hashimoto A, Hayashi M, Madokoro N, Asano Y, Abe M, Ishii T, Isei T, Ito T, Inoue Y, Imafuku S, Irisawa R, Ohtsuka M, Ogawa F, Kadono T, Kawakami T, Kukino R, Kono T, Kodera M, Takahara M, Tanioka M, Nakanishi T, Nakamura Y, Hasegawa M, Fujimoto M, Fujiwara H, Maekawa T, Matsuo K, Yamasaki O, Le Pavoux A, Tachibana T, Ihn H, Wound/Burn Guidelines Committee

    The Journal of dermatology   43 ( 9 )   989 - 1010   2016年09月( ISSN:0385-2407

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/1346-8138.13288

    PubMed

  • The wound/burn guidelines - 5: Guidelines for the management of lower leg ulcers/varicose veins. 査読

    Ito T, Kukino R, Takahara M, Tanioka M, Nakamura Y, Asano Y, Abe M, Ishii T, Isei T, Inoue Y, Imafuku S, Irisawa R, Ohtsuka M, Ohtsuka M, Ogawa F, Kadono T, Kawakami T, Kawaguchi M, Kono T, Kodera M, Sakai K, Nakanishi T, Hashimoto A, Hasegawa M, Hayashi M, Fujimoto M, Fujiwara H, Maekawa T, Matsuo K, Madokoro N, Yamasaki O, Yoshino Y, Le Pavoux A, Tachibana T, Ihn H, Wound/Burn Guidelines Committee

    The Journal of dermatology   43 ( 8 )   853 - 68   2016年08月( ISSN:0385-2407

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/1346-8138.13286

    PubMed

  • The wound/burn guidelines - 4: Guidelines for the management of skin ulcers associated with connective tissue disease/vasculitis. 査読

    Fujimoto M, Asano Y, Ishii T, Ogawa F, Kawakami T, Kodera M, Abe M, Isei T, Ito T, Inoue Y, Imafuku S, Irisawa R, Ohtsuka M, Ohtsuka M, Kadono T, Kawaguchi M, Kukino R, Kono T, Sakai K, Takahara M, Tanioka M, Nakanishi T, Nakamura Y, Hashimoto A, Hasegawa M, Hayashi M, Fujiwara H, Maekawa T, Matsuo K, Madokoro N, Yamasaki O, Yoshino Y, Le Pavoux A, Tachibana T, Ihn H, Wound/Burn Guidelines Committee

    The Journal of dermatology   43 ( 7 )   729 - 57   2016年07月( ISSN:0385-2407

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/1346-8138.13275

    PubMed

  • Generation of Rat Monoclonal Antibodies Against Human Pancreatic Ductal Adenocarcinoma Cells. 査読

    Higashi K, Fujii N, Kushida M, Yamada K, Suzuki N, Saito K, Tachibana T

    Monoclonal antibodies in immunodiagnosis and immunotherapy   35 ( 3 )   148 - 154   2016年06月( ISSN:2167-9436

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/mab.2016.0012

    PubMed

  • Generation of Rat Monoclonal Antibodies Against Human Pancreatic Ductal Adenocarcinoma Cells. 査読

    Higashi K, Fujii N, Kushida M, Yamada K, Suzuki N, Saito K, Tachibana T

    Monoclonal antibodies in immunodiagnosis and immunotherapy   35 ( 3 )   148 - 54   2016年06月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/mab.2016.0012

    PubMed

  • The wound/burn guidelines - 3: Guidelines for the diagnosis and treatment for diabetic ulcer/gangrene. 査読

    Isei T, Abe M, Nakanishi T, Matsuo K, Yamasaki O, Asano Y, Ishii T, Ito T, Inoue Y, Imafuku S, Irisawa R, Ohtsuka M, Ohtsuka M, Ogawa F, Kadono T, Kodera M, Kawakami T, Kawaguchi M, Kukino R, Kono T, Sakai K, Takahara M, Tanioka M, Nakamura Y, Hashimoto A, Hasegawa M, Hayashi M, Fujimoto M, Fujiwara H, Maekawa T, Madokoro N, Yoshino Y, Le Pavoux A, Tachibana T, Ihn H, Wound/Burn Guidelines Committee

    The Journal of dermatology   43 ( 6 )   591 - 619   2016年06月( ISSN:0385-2407

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/1346-8138.13285

    PubMed

  • The wound/burn guidelines - 2: Guidelines for the diagnosis and treatment for pressure ulcers. 査読

    Tachibana T, Imafuku S, Irisawa R, Ohtsuka M, Kadono T, Fujiwara H, Asano Y, Abe M, Ishii T, Isei T, Ito T, Inoue Y, Ohtsuka M, Ogawa F, Kodera M, Kawakami T, Kawaguchi M, Kukino R, Kono T, Sakai K, Takahara M, Tanioka M, Nakanishi T, Nakamura Y, Hashimoto A, Hasegawa M, Hayashi M, Fujimoto M, Maekawa T, Matsuo K, Madokoro N, Yamasaki O, Yoshino Y, Le Pavoux A, Ihn H, Wound/Burn Guidelines Committee

    The Journal of dermatology   43 ( 5 )   469 - 506   2016年05月( ISSN:0385-2407

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/1346-8138.13274

    PubMed

  • The wound/burn guidelines - 1: Wounds in general. 査読

    Inoue Y, Hasegawa M, Maekawa T, Le Pavoux A, Asano Y, Abe M, Ishii T, Ito T, Isei T, Imafuku S, Irisawa R, Ohtsuka M, Ohtsuka M, Ogawa F, Kadono T, Kodera M, Kawakami T, Kawaguchi M, Kukino R, Kono T, Sakai K, Takahara M, Tanioka M, Nakanishi T, Nakamura Y, Hashimoto A, Hayashi M, Fujimoto M, Fujiwara H, Matsuo K, Madokoro N, Yamasaki O, Yoshino Y, Tachibana T, Ihn H, Wound/Burn Guidelines Committee

    The Journal of dermatology   43 ( 4 )   357 - 75   2016年04月( ISSN:0385-2407

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/1346-8138.13276

    PubMed

  • Chromatin-prebound Crm1 recruits Nup98-HoxA9 fusion to induce aberrant expression of Hox cluster genes 査読

    Oka Masahiro, Mura Sonoko, Yamada Kohji, Sangel Percival, Hirata Saki, Maehara Kazumitsu, Kawakami Koichi, Tachibana Taro, Ohkawa Yasuyuki, Kimura Hiroshi, Yoneda Yoshihiro

    ELIFE   5   2016年01月( ISSN:2050-084X

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.7554/eLife.09540

  • Chromatin-prebound Crm1 recruits Nup98-HoxA9 fusion to induce aberrant expression of Hox cluster genes. 査読

    Oka M, Mura S, Yamada K, Sangel P, Hirata S, Maehara K, Kawakami K, Tachibana T, Ohkawa Y, Kimura H, Yoneda Y

    eLife   5   e09540   2016年01月( ISSN:2050-084X

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.7554/eLife.09540

    PubMed

  • Generation and Characterization of Rat Monoclonal Antibodies Against Epidermal Growth Factor Receptor. 査読

    Osaki T, Wang CX, Tachibana T, Azuma M, Kitamura M, Nakanishi T

    Monoclonal antibodies in immunodiagnosis and immunotherapy   34 ( 6 )   418 - 422   2015年12月( ISSN:2167-9436

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/mab.2015.0034

    PubMed

  • Generation and Characterization of Rat Monoclonal Antibodies Against Epidermal Growth Factor Receptor. 査読

    Osaki T, Wang CX, Tachibana T, Azuma M, Kitamura M, Nakanishi T

    Monoclonal antibodies in immunodiagnosis and immunotherapy   34 ( 6 )   418 - 22   2015年12月

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/mab.2015.0034

    PubMed

  • Phosphorylation of myosin II regulatory light chain by ZIP kinase is responsible for cleavage furrow ingression during cell division in mammalian cultured cells 査読

    Hosoba Kosuke, Komatsu Satoshi, Ikebe Mitsuo, Kotani Manato, Xiao Wenqin, Tachibana Taro, Hosoya Hiroshi, Hamao Kozue

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   459 ( 4 )   686 - 691   2015年04月( ISSN:0006-291X

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.bbrc.2015.03.005

  • Nuclear import of the U1A splicesome protein is mediated by importin α/β and Ran in living mammalian cells. 査読

    Hieda M, Tachibana T, Fukumoto M, Yoneda Y

    The Journal of biological chemistry   290 ( 16 )   9948   2015年04月( ISSN:0021-9258

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1074/jbc.A115.008299

    PubMed

  • Nuclear import of the U1A splicesome protein is mediated by importin α/β and Ran in living mammalian cells. 査読

    Hieda M, Tachibana T, Fukumoto M, Yoneda Y

    The Journal of biological chemistry   290 ( 16 )   9948 - 9948   2015年04月( ISSN:0021-9258

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1074/jbc.A115.008299

    PubMed

  • Phosphorylation of myosin II regulatory light chain by ZIP kinase is responsible for cleavage furrow ingression during cell division in mammalian cultured cells. 査読

    Hosoba K, Komatsu S, Ikebe M, Kotani M, Wenqin X, Tachibana T, Hosoya H, Hamao K

    Biochemical and biophysical research communications   459 ( 4 )   686 - 691   2015年04月( ISSN:0006-291X

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.bbrc.2015.03.005

    PubMed

  • DBZ Regulates Cortical Cell Positioning and Neurite Development by Sustaining the Anterograde Transport of Lis1 and DISC1 through Control of Ndel1 Dual-Phosphorylation 査読

    Okamoto Masayuki, Iguchi Tokuichi, Hattori Tsuyoshi, Matsuzaki Shinsuke, Koyama Yoshihisa, Taniguchi Manabu, Komada Munekazu, Xie Min-Jue, Yagi Hideshi, Shimizu Shoko, Konishi Yoshiyuki, Omi Minoru, Yoshimi Tomohiko, Tachibana Taro, Fujieda Shigeharu, Katayama Taiichi, Ito Akira, Hirotsune Shinji, Tohyama Masaya, Sato Makoto

    JOURNAL OF NEUROSCIENCE   35 ( 7 )   2942 - 2958   2015年02月( ISSN:0270-6474

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1523/JNEUROSCI.5029-13.2015

  • A Novel Marker for Undifferentiated Human Embryonic Stem Cells 査読

    Kiyoshi Higashi, Masaki Yagi, Tatsuhiko Arakawa, Kouji Asano, Kumiko Kobayashi, Taro Tachibana, Koichi Saito

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   34 ( 1 )   7 - 11   2015年02月( ISSN:2167-9436

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    掲載種別:研究論文(学術雑誌)  

    Human embryonic stem cells (hESCs) are pluripotent stem cells from early embryos, and their self-renewal capacity depends on the sustained expression of hESC-specific molecules and the suppressed expression of differentiation-associated genes. To discover novel molecules expressed on hESCs, we generated a panel of monoclonal antibodies against undifferentiated hESCs. The antigen recognized by MAb2 is expressed on the cell surface of undifferentiated hESCs
    three diffused bands with molecular mass between 30 and 60kDa in the lysates of hESCs were diminished during hESC differentiation into neural cells. The expression of MAb2 antigen was also observed on the plasma membrane of lung cancer cells, and MAb2 detected 55, 50, and 35kDa protein bands in the cell lysates. Immunoprecipitation followed by proteomics analyses identified CD147/basigin as a MAb2 antigen. Finally, the positive expression of CD147/basigin protein in undifferentiated hESCs was confirmed. These results suggested that CD147/basigin could be another undifferentiated hESC marker.

    DOI: 10.1089/mab.2014.0075

    PubMed

  • A novel marker for undifferentiated human embryonic stem cells. 査読

    Higashi K, Yagi M, Arakawa T, Asano K, Kobayashi K, Tachibana T, Saito K

    Monoclonal antibodies in immunodiagnosis and immunotherapy   34 ( 1 )   7 - 11   2015年02月

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/mab.2014.0075

    PubMed

  • DBZ regulates cortical cell positioning and neurite development by sustaining the anterograde transport of Lis1 and DISC1 through control of Ndel1 dual-phosphorylation. 査読

    Okamoto M, Iguchi T, Hattori T, Matsuzaki S, Koyama Y, Taniguchi M, Komada M, Xie MJ, Yagi H, Shimizu S, Konishi Y, Omi M, Yoshimi T, Tachibana T, Fujieda S, Katayama T, Ito A, Hirotsune S, Tohyama M, Sato M

    The Journal of neuroscience : the official journal of the Society for Neuroscience   35 ( 7 )   2942 - 2958   2015年02月( ISSN:0270-6474

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1523/JNEUROSCI.5029-13.2015

    PubMed

  • Incorporation of histone H3.1 suppresses the lineage potential of skeletal muscle 査読

    Harada Akihito, Maehara Kazumitsu, Sato Yuko, Konno Daijiro, Tachibana Taro, Kimura Hiroshi, Ohkawa Yasuyuki

    NUCLEIC ACIDS RESEARCH   43 ( 2 )   775 - 786   2015年01月( ISSN:0305-1048

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/nar/gku1346

  • Incorporation of histone H3.1 suppresses the lineage potential of skeletal muscle. 査読

    Harada A, Maehara K, Sato Y, Konno D, Tachibana T, Kimura H, Ohkawa Y

    Nucleic acids research   43 ( 2 )   775 - 786   2015年01月( ISSN:0305-1048

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/nar/gku1346

    PubMed

  • Expression of the Clustered NeuAc alpha 2-3Gal beta O-Glycan Determines the Cell Differentiation State of the Cells 査読

    Higashi Kiyoshi, Asano Kouji, Yagi Masaki, Yamada Keita, Arakawa Tatsuhiko, Ehashi Tomo, Mori Takashi, Sumida Kayo, Kushida Masahiko, Ando Satoshi, Kinoshita Mitsuhiro, Kakehi Kazuaki, Tachibana Taro, Saito Koichi

    JOURNAL OF BIOLOGICAL CHEMISTRY   289 ( 37 )   25833 - 25843   2014年09月( ISSN:0021-9258

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1074/jbc.M114.550848

  • Expression of the clustered NeuAcα2-3Galβ O-glycan determines the cell differentiation state of the cells. 査読

    Higashi K, Asano K, Yagi M, Yamada K, Arakawa T, Ehashi T, Mori T, Sumida K, Kushida M, Ando S, Kinoshita M, Kakehi K, Tachibana T, Saito K

    The Journal of biological chemistry   289 ( 37 )   25833 - 25843   2014年09月( ISSN:0021-9258

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1074/jbc.M114.550848

    PubMed

  • N-ethylmaleimide-sensitive factor interacts with the serotonin transporter and modulates its trafficking: implications for pathophysiology in autism 査読

    Iwata Keiko, Matsuzaki Hideo, Tachibana Taro, Ohno Koji, Yoshimura Saori, Takamura Hironori, Yamada Kohei, Matsuzaki Shinsuke, Nakamura Kazuhiko, Tsuchiya Kenji J., Matsumoto Kaori, Tsujii Masatsugu, Sugiyama Toshirou, Katayama Taiichi, Mori Norio

    MOLECULAR AUTISM   5   2014年05月( ISSN:2040-2392

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1186/2040-2392-5-33

  • KRAS mutation confers resistance to antibody-dependent cellular cytotoxicity of cetuximab against human colorectal cancer cells 査読

    Nakadate Yusuke, Kodera Yasuo, Kitamura Yuka, Shirasawa Senji, Tachibana Taro, Tamura Tomohide, Koizumi Fumiaki

    INTERNATIONAL JOURNAL OF CANCER   134 ( 9 )   2146 - 2155   2014年05月( ISSN:0020-7136

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1002/ijc.28550

  • DBZ, a CNS- Specific DISC1 Binding Protein, Positively Regulates Oligodendrocyte Differentiation 査読

    Shimizu Shoko, Koyama Yoshihisa, Hattori Tsuyoshi, Tachibana Taro, Yoshimi Tomohiko, Emoto Hisayo, Matsumoto Yuji, Miyata Shingo, Katayama Taiichi, Ito Akira, Tohyama Masaya

    GLIA   62 ( 5 )   709 - 724   2014年05月( ISSN:0894-1491

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1002/glia.22636

  • Isolation of diploid baker's yeast capable of strongly activating immune cells and analyses of the cell wall structure 査読

    Takada Yuki, Mizobuchi Ayano, Kato Takayuki, Kasahara Emiko, Ito Chinatsu, Watanabe Hajime, Kanzaki Ken, Kitagawa Seiichi, Tachibana Taro, Azuma Masayuki

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   78 ( 5 )   911 - 915   2014年05月( ISSN:0916-8451

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1080/09168451.2014.917259

  • DBZ, a CNS- Specific DISC1 Binding Protein, Positively Regulates Oligodendrocyte Differentiation 査読

    Shoko Shimizu, Yoshihisa Koyama, Tsuyoshi Hattori, Taro Tachibana, Tomohiko Yoshimi, Hisayo Emoto, Yuji Matsumoto, Shingo Miyata, Taiichi Katayama, Akira Ito, Masaya Tohyama

    GLIA   62 ( 5 )   709 - 724   2014年05月( ISSN:0894-1491

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    掲載種別:研究論文(学術雑誌)  

    Recent studies have shown changes in myelin genes and alterations in white matter structure in a wide range of psychiatric disorders. Here we report that DBZ, a central nervous system (CNS)-specific member of the DISC1 interactome, positively regulates the oligodendrocyte (OL) differentiation in vivo and in vitro. In mouse corpus callosum (CC), DBZ mRNA is expressed in OL lineage cells and expression of DBZ protein peaked before MBP expression. In the CC of DBZ-KO mice, we observed delayed myelination during the early postnatal period. Although the myelination delay was mostly recovered by adulthood, OLs with immature structural features were more abundant in adult DBZ-KO mice than in control mice. DBZ was also transiently upregulated during rat OL differentiation in vitro before myelin marker expression. DBZ knockdown by RNA interference resulted in a decreased expression of myelin-related markers and a low number of cells with mature characteristics, but with no effect on the proliferation of oligodendrocyte precursor cells. We also show that the expression levels of transcription factors having a negative-regulatory role in OL differentiation were upregulated when endogenous DBZ was knocked down. These results strongly indicate that OL differentiation in rodents is regulated by DBZ.

    DOI: 10.1002/glia.22636

  • N-ethylmaleimide-sensitive factor interacts with the serotonin transporter and modulates its trafficking: implications for pathophysiology in autism. 査読

    Iwata K, Matsuzaki H, Tachibana T, Ohno K, Yoshimura S, Takamura H, Yamada K, Matsuzaki S, Nakamura K, Tsuchiya KJ, Matsumoto K, Tsujii M, Sugiyama T, Katayama T, Mori N

    Molecular autism   5   33   2014年05月( ISSN:2040-2392

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1186/2040-2392-5-33

    PubMed

  • KRAS mutation confers resistance to antibody-dependent cellular cytotoxicity of cetuximab against human colorectal cancer cells 査読

    Yusuke Nakadate, Yasuo Kodera, Yuka Kitamura, Senji Shirasawa, Taro Tachibana, Tomohide Tamura, Fumiaki Koizumi

    INTERNATIONAL JOURNAL OF CANCER   134 ( 9 )   2146 - 2155   2014年05月( ISSN:0020-7136

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    掲載種別:研究論文(学術雑誌)  

    Cetuximab is a chimeric IgG1 monoclonal antibody (mAb) that targets the extracellular domain of epidermal growth factor receptor (EGFR). Oncogenic KRAS mutations in tumors have been shown to be a negative predictor of the response of colorectal cancer (CRC) to cetuximab treatment. Cetuximab exerts its therapeutic effects through several mechanisms including antibody-dependent cellular cytotoxicity (ADCC). However, the influence of KRAS mutations on cetuximab-mediated ADCC is not fully understood. Here, we investigated cetuximab-mediated ADCC in two pairs of isogenic CRC cells with or without a KRAS mutation. Peripheral blood mononuclear cells (PBMCs) from healthy volunteers and NK92, a natural killer (NK) cell line that exogenously expresses Fc?RIIIa (CD16a), were used as effector cells. In an ADCC assay, perforin-dependent target cell lysis was not affected by the KRAS mutation status. On the other hand, perforin-independent ADCC was observed only in CRC cells with wild-type KRAS, but not in cells with mutant KRAS. Neutralizing experiments revealed that the Fas-Fas ligand (FasL) interaction was responsible for the induction of apoptosis and perforin-independent ADCC. Furthermore, the presence of effector cells clearly enhanced the growth-inhibitory effect of cetuximab only in CRC cells with wild-type KRAS, but not in those with mutant KRAS. These findings suggest that ADCC is an important mode of action of cetuximab and that KRAS mutation impairs the therapeutic effect exerted by cetuximab-mediated ADCC.

    DOI: 10.1002/ijc.28550

  • Isolation of diploid baker's yeast capable of strongly activating immune cells and analyses of the cell wall structure. 査読

    Takada Y, Mizobuchi A, Kato T, Kasahara E, Ito C, Watanabe H, Kanzaki K, Kitagawa S, Tachibana T, Azuma M

    Bioscience, biotechnology, and biochemistry   78 ( 5 )   911 - 915   2014年05月( ISSN:0916-8451

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1080/09168451.2014.917259

    PubMed

  • 免疫細胞を強力に活性化できる2倍体パン用イーストの分離およびその細胞壁構造の解析(Isolation of diploid baker's yeast capable of strongly activating immune cells and analyses of the cell wall structure) 査読

    Takada Yuki, Mizobuchi Ayano, Kato Takayuki, Kasahara Emiko, Ito Chinatsu, Watanabe Hajime, Kanzaki Ken, Kitagawa Seiichi, Tachibana Taro, Azuma Masayuki

    Bioscience, Biotechnology, and Biochemistry   78 ( 5 )   911 - 915   2014年05月( ISSN:0916-8451

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    掲載種別:研究論文(学術雑誌)  

    ハプロイド突然変異体AQ-37に基づいて、マウスマクロファージを強力に活性化できる2倍体パン用イーストを構築し、その活性化に対する要因を検討した。得られた3種の2倍体株(BQ-10、BQ-48、BQ-55)のうち、BQ-55が最高のマクロファージ活性化能を示したため、2倍体パン用イーストとして詳細な評価を行った。BQ-55の増殖能はAQ-37より約5倍高かった。2倍体株BQ-55はヒト免疫細胞も活性化した。マウスRAW264.7細胞およびヒト末梢血単核球細胞において、BQ-55はTNF-α分泌を有意に促進した。この活性化に対する要因を解明するため、その細胞壁構造、特にβグルカン構造を解析した結果、分岐の長さ、β-1,6-グルカンがその要因の一つであることが示唆された。

  • Generation of a monoclonal antibody against the extracellular domain of IGF-1R. 査読

    Nakadate Y, Yoshimura S, Nakatani N, Azuma M, Tachibana T

    Monoclonal antibodies in immunodiagnosis and immunotherapy   33 ( 2 )   126 - 128   2014年04月( ISSN:2167-9436

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/mab.2013.0083

    PubMed

  • Generation of a monoclonal antibody against the extracellular domain of IGF-1R. 査読

    Nakadate Y, Yoshimura S, Nakatani N, Azuma M, Tachibana T

    Monoclonal antibodies in immunodiagnosis and immunotherapy   33 ( 2 )   126 - 8   2014年04月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/mab.2013.0083

    PubMed

  • Isolation and characterization of baker's yeast capable of strongly activating a macrophage 査読

    Takada Yuki, Nishino Yumiko, Ito Chinatsu, Watanabe Hajime, Kanzaki Ken, Tachibana Taro, Azuma Masayuki

    FEMS YEAST RESEARCH   14 ( 2 )   261 - 269   2014年03月( ISSN:1567-1356

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/1567-1364.12098

  • Isolation and characterization of baker's yeast capable of strongly activating a macrophage. 査読

    Takada Y, Nishino Y, Ito C, Watanabe H, Kanzaki K, Tachibana T, Azuma M

    FEMS yeast research   14 ( 2 )   261 - 269   2014年03月( ISSN:1567-1356

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/1567-1364.12098

    PubMed

  • A milliliter-scale yeast-based fuel cell with high performance 査読

    Kaneshiro Hiroyuki, Takano Kosuke, Takada Yogo, Wakisaka Tomoyuki, Tachibana Taro, Azuma Masayuki

    BIOCHEMICAL ENGINEERING JOURNAL   83   90 - 96   2014年02月( ISSN:1369-703X

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.bej.2013.12.011

  • Generation of a monoclonal antibody for INI1/hSNF5/BAF47 査読

    Akihito Harada, Masayasu Hayashi, Yuuki Kuniyoshi, Yuichiro Semba, Satoko Sugahara, Taro Tachibana, Yasuyuki Ohkawa, Masatoshi Fujita

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   33 ( 1 )   49 - 51   2014年02月( ISSN:2167-9436

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    掲載種別:研究論文(学術雑誌)  

    INI1/hSNF5/BAF47, which has an SNF5 domain, belongs to the SWI/SNF family. This family is known as ATP-dependent regulators of gene expression by remodeling chromatin structure during cell differentiation. However, the detailed function of INI1/hSNF5/BAF47 is unclear. Here we report the generation of a specific monoclonal antibody for INI1/hSNF5/BAF47 by the mouse iliac lymph node method. The obtained antibody recognized two isoforms of INI1/hSNF5/BAF47 in immunoblotting and precisely recognized the nuclear localization of INI1/hSNF5/BAF47 in immunostaining. This antibody can contribute to further elucidation of the mechanisms of gene expression regulation by INI1/hSNF5/BAF47 during cell differentiation. © 2014, Mary Ann Liebert, Inc.

    DOI: 10.1089/mab.2013.0065

    PubMed

  • Production of a monoclonal antibody for C/EBPβ: the subnuclear localization of C/EBPβ in mouse L929 cells. 査読

    Harada A, Okazaki E, Okada S, Tachibana T, Ohkawa Y

    Monoclonal antibodies in immunodiagnosis and immunotherapy   33 ( 1 )   34 - 37   2014年02月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/mab.2013.0069

    PubMed

  • Cell wall structure suitable for surface display of proteins in Saccharomyces cerevisiae 査読

    Matsuoka Hiroyuki, Hashimoto Kazuya, Saijo Aki, Takada Yuki, Kondo Akihiko, Ueda Mitsuyoshi, Ooshima Hiroshi, Tachibana Taro, Azuma Masayuki

    YEAST   31 ( 2 )   67 - 76   2014年02月( ISSN:0749-503X

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1002/yea.2995

  • Production of a monoclonal antibody for C/EBPβ: the subnuclear localization of C/EBPβ in mouse L929 cells. 査読

    Harada A, Okazaki E, Okada S, Tachibana T, Ohkawa Y

    Monoclonal antibodies in immunodiagnosis and immunotherapy   33 ( 1 )   34 - 7   2014年02月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/mab.2013.0069

    PubMed

  • Generation of a monoclonal antibody for INI1/hSNF5/BAF47. 査読

    Harada A, Hayashi M, Kuniyoshi Y, Semba Y, Sugahara S, Tachibana T, Ohkawa Y, Fujita M

    Monoclonal antibodies in immunodiagnosis and immunotherapy   33 ( 1 )   49 - 51   2014年02月

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/mab.2013.0065

    PubMed

  • A milliliter-scale yeast-based fuel cell with high performance 査読

    Hiroyuki Kaneshiro, Kosuke Takano, Yogo Takada, Tomoyuki Wakisaka, Taro Tachibana, Masayuki Azuma

    BIOCHEMICAL ENGINEERING JOURNAL   83   90 - 96   2014年02月( ISSN:1369-703X

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    掲載種別:研究論文(学術雑誌)  

    Microbial fuel cells are attracting attention as one of the systems for producing electrical energy from organic compounds. We used commercial baker's yeast (Saccharomyces cerevisiae) for a glucose fuel cell because the yeast is a safe organism and relatively high power can be generated in the system. In the present study, a milliliter (mL)-scale dual-chamber fuel cell was constructed for evaluating the power generated by a variety of yeasts and their mutants, and the optimum conditions for high performance were investigated. When carbon fiber bundles were used as an electrode in the fuel cell, high volumetric power density was obtained. The maximum power produced per volume of anode solution was 850 W/m(3) under optimum conditions. Furthermore, the power was examined using seven kinds of yeast. In Kluyveromyces marxianus, not only the power but also the power per consumed glucose was high. Moreover, it was suggested that xylose is available as fuel for the fuel cell. The fuel cell powered by K. marxianus may prove to be helpful for the effective utilization of woody biomass. (C) 2014 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.bej.2013.12.011

  • Cell wall structure suitable for surface display of proteins in Saccharomyces cerevisiae. 査読

    Matsuoka H, Hashimoto K, Saijo A, Takada Y, Kondo A, Ueda M, Ooshima H, Tachibana T, Azuma M

    Yeast (Chichester, England)   31 ( 2 )   67 - 76   2014年02月( ISSN:0749-503X

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1002/yea.2995

    PubMed

  • Silencing of poly(ADP-ribose) glycohydrolase sensitizes lung cancer cells to radiation through the abrogation of DNA damage checkpoint 査読

    Nakadate Yusuke, Kodera Yasuo, Kitamura Yuka, Tachibana Taro, Tamura Tomohide, Koizumi Fumiaki

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   441 ( 4 )   793 - 798   2013年11月( ISSN:0006-291X

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.bbrc.2013.10.134

  • Silencing of poly(ADP-ribose) glycohydrolase sensitizes lung cancer cells to radiation through the abrogation of DNA damage checkpoint. 査読

    Nakadate Y, Kodera Y, Kitamura Y, Tachibana T, Tamura T, Koizumi F

    Biochemical and biophysical research communications   441 ( 4 )   793 - 798   2013年11月( ISSN:0006-291X

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.bbrc.2013.10.134

    PubMed

  • Functional activity of RLIM/Rnf12 is regulated by phosphorylation-dependent nucleocytoplasmic shuttling. 査読

    Jiao B, Taniguchi-Ishigaki N, Güngör C, Peters MA, Chen YW, Riethdorf S, Drung A, Ahronian LG, Shin J, Pagnis R, Pantel K, Tachibana T, Lewis BC, Johnsen SA, Bach I

    Molecular biology of the cell   24 ( 19 )   3085 - 96   2013年10月( ISSN:1059-1524

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1091/mbc.E13-05-0239

    PubMed

  • Functional activity of RLIM/Rnf12 is regulated by phosphorylation-dependent nucleocytoplasmic shuttling 査読

    Baowei Jiao, Naoko Taniguchi-Ishigaki, Cenap Guengoer, Marvin A. Peters, Ya-Wen Chen, Sabine Riethdorf, Alexander Drung, Leanne G. Ahronian, JongDae Shin, Rachna Pagnis, Klaus Pantel, Taro Tachibana, Brian C. Lewis, Steven A. Johnsen, Ingolf Bach

    MOLECULAR BIOLOGY OF THE CELL   24 ( 19 )   3085 - 3096   2013年10月( ISSN:1059-1524

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    掲載種別:研究論文(学術雑誌)  

    The X-linked gene Rnf12 encodes the ubiquitin ligase really interesting new gene (RING) finger LIM domain-interacting protein (RLIM)/RING finger protein 12 (Rnf12), which serves as a major sex-specific epigenetic regulator of female mouse nurturing tissues. Early during embryogenesis, RLIM/Rnf12 expressed from the maternal allele is crucial for the development of extraembryonic trophoblast cells. In contrast, in mammary glands of pregnant and lactating adult females RLIM/Rnf12 expressed from the paternal allele functions as a critical survival factor for milk-producing alveolar cells. Although RLIM/Rnf12 is detected mostly in the nucleus, little is known about how and in which cellular compartment(s) RLIM/Rnf12 mediates its biological functions. Here we demonstrate that RLIM/Rnf12 protein shuttles between nucleus and cytoplasm and this is regulated by phosphorylation of serine S214 located within its nuclear localization sequence. We show that shuttling is important for RLIM to exert its biological functions, as alveolar cell survival activity is inhibited in cells expressing shuttling-deficient nuclear or cytoplasmic RLIM/Rnf12. Thus regulated nucleocytoplasmic shuttling of RLIM/Rnf12 coordinates cellular compartments during mammary alveolar cell survival.

    DOI: 10.1091/mbc.E13-05-0239

    PubMed

  • Altered expression of dermokine in skin disorders 査読

    Hasegawa M., Higashi K., Yokoyama C., Yamamoto F., Tachibana T., Matsushita T., Hamaguchi Y., Saito K., Fujimoto M., Takehara K.

    JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY   27 ( 7 )   867 - 875   2013年07月( ISSN:0926-9959

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/j.1468-3083.2012.04598.x

  • Altered expression of dermokine in skin disorders 査読

    M. Hasegawa, K. Higashi, C. Yokoyama, F. Yamamoto, T. Tachibana, T. Matsushita, Y. Hamaguchi, K. Saito, M. Fujimoto, K. Takehara

    Journal of the European Academy of Dermatology and Venereology   27 ( 7 )   867 - 875   2013年07月( ISSN:0926-9959

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    掲載種別:研究論文(学術雑誌)  

    Background Although dermokine-β, a glycoprotein expressed in epithelial cells, does not have significant homology to other proteins, its carboxyl-terminal domain shares a high pI value with many cytokines, suggesting similar functions. Objective To better understand the biology of dermokine, we here determined its localization under pathological conditions and examined factors that regulate its expression. Methods We generated an anti-human dermokine-β/γ monoclonal antibody cross-reacting with the mouse protein. Using this antibody, immunohistological staining and Western blotting of dermokine-β/γ were performed with various tissue samples. Results Although human dermokine-β/γ was expressed in almost all granular layers, upper spinous layers of the skin were also stained with anti-dermokine-β/γ antibody in inflammatory skin disorders. Dermokine-β/γ was expressed in keratoacanthoma and a part of well-differentiated squamous cell carcinoma (SCC). However, dermokine-β/ γ was not detected in poorly differentiated SCC or tumours derived from non-keratinocytes. In mice, dermokine-β/γ-expressed keratinocytes were increased in models of contact hypersensitivity, ultraviolet-irradiated skin injury and wound healing. Consistent with expanded distribution in inflammatory skin diseases, proinflammatory cytokines such as interleukin-1β, interleukin-12, and tumour necrosis factor-α augmented dermokine-β/γ expression in cultured human keratinocytes. In contrast, growth factors including epidermal growth factor, insulin-like growth factor-I, keratinocyte growth factor and transforming growth factor-α significantly reduced dermokine expression. Conclusion These results provide novel insights into the physiological and pathological significance of dermokine in the epidermis. © 2012 European Academy of Dermatology and Venereology.

    DOI: 10.1111/j.1468-3083.2012.04598.x

    PubMed

  • Production of a monoclonal antibody specific for Pou5f1/Oct4. 査読

    Arakawa T, Yoshimi T, Azuma M, Tachibana T

    Monoclonal antibodies in immunodiagnosis and immunotherapy   32 ( 3 )   229 - 231   2013年06月( ISSN:2167-9436

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/mab.2013.0004

    PubMed

  • Production of a monoclonal antibody specific for Pou5f1/Oct4. 査読

    Arakawa T, Yoshimi T, Azuma M, Tachibana T

    Monoclonal antibodies in immunodiagnosis and immunotherapy   32 ( 3 )   229 - 31   2013年06月

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/mab.2013.0004

    PubMed

  • A panel of specific monoclonal antibodies directed against various phosphorylated histones H3. 査読

    Yoshimi T, Ohkawa Y, Azuma M, Tachibana T

    Monoclonal antibodies in immunodiagnosis and immunotherapy   32 ( 2 )   119 - 124   2013年04月( ISSN:2167-9436

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/mab.2012.0105

    PubMed

  • Production of a Monoclonal Antibody Specific for Pou5f1/Oct4.

    Arakawa T, Yoshimi T, Azuma M, Tachibana T.

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy,   2013年04月

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • A panel of specific monoclonal antibodies directed against various phosphorylated histones H3. 査読

    Yoshimi T, Ohkawa Y, Azuma M, Tachibana T

    Monoclonal antibodies in immunodiagnosis and immunotherapy   32 ( 2 )   119 - 24   2013年04月

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/mab.2012.0105

    PubMed

  • Production of a Monoclonal Antibody Specific for Pou5f1/Oct4. 査読

    Arakawa T, Yoshimi T, Azuma M, Tachibana T.

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy,   2013年04月

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • A panel of specific monoclonal antibodies directed against various phosphorylated histone H3.

    Yoshimi T., Ohkawa Y., Azuma M., Tachibana T.

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy,   2013年03月

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • A panel of specific monoclonal antibodies directed against various phosphorylated histone H3. 査読

    Yoshimi T., Ohkawa Y., Azuma M., Tachibana T.

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy,   2013年03月

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Substrate specificity of Pasteurella multocida toxin for alpha subunits of heterotrimeric G proteins 査読

    Orth Joachim H. C., Fester Ines, Siegert Peter, Weise Markus, Lanner Ulrike, Kamitani Shigeki, Tachibana Taro, Wilson Brenda A., Schlosser Andreas, Horiguchi Yasuhiko, Aktories Klaus

    FASEB JOURNAL   27 ( 2 )   832 - 842   2013年02月( ISSN:0892-6638

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1096/fj.12-213900

  • Substrate specificity of Pasteurella multocida toxin for alpha subunits of heterotrimeric G proteins 査読

    Orth Joachim H. C, Fester Ines, Siegert Peter, Weise Markus, Lanner Ulrike, Kamitani Shigeki, Tachibana Taro, Wilson Brenda A, Schlosser Andreas, Horiguchi Yasuhiko, Aktories Klaus

    FASEB JOURNAL   27 ( 2 )   832 - 842   2013年02月( ISSN:0892-6638

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1096/fj.12-213900

    PubMed

  • Contribution of Leydig and Sertoli cells to testosterone production in mouse fetal testes.

    Shima Y, Miyabayashi K, Haraguchi S, Arakawa T, et al.,

    Mol Endocrinol   27   63 - 73   2013年01月

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    掲載種別:研究論文(学術雑誌)  

  • Contribution of Leydig and Sertoli Cells to Testosterone Production in Mouse Fetal Testes 査読

    Shima Yuichi, Miyabayashi Kanako, Haraguchi Shogo, Arakawa Tatsuhiko, Otake Hiroyuki, Baba Takashi, Matsuzaki Sawako, Shishido Yurina, Akiyama Haruhiko, Tachibana Taro, Tsutsui Kazuyoshi, Morohashi Ken-ichirou

    MOLECULAR ENDOCRINOLOGY   27 ( 1 )   63 - 73   2013年01月( ISSN:0888-8809

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1210/me.2012-1256

  • A co-localization model of paired ChIP-seq data using a large ENCODE data set enables comparison of multiple samples 査読

    Maehara Kazumitsu, Odawara Jun, Harada Akihito, Yoshimi Tomohiko, Nagao Koji, Obuse Chikashi, Akashi Koichi, Tachibana Taro, Sakata Toshio, Ohkawa Yasuyuki

    NUCLEIC ACIDS RESEARCH   41 ( 1 )   54 - 62   2013年01月( ISSN:0305-1048

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/nar/gks1010

  • A co-localization model of paired ChIP-seq data using a large ENCODE data set enables comparison of multiple samples.

    Maehara K., Odawara J., Harada A., Yoshimi T., Nagao K., Obuse C., Akashi K., Tachibana T., Sakata T., Ohkawa Y.

    Nucleic Acids Res.   41   54 - 62   2013年01月

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    掲載種別:研究論文(学術雑誌)  

  • Contribution of Leydig and Sertoli cells to testosterone production in mouse fetal testes. 査読

    Shima Y, Miyabayashi K, Haraguchi S, Arakawa T, et al.,

    Mol Endocrinol   27   63 - 73   2013年01月

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    掲載種別:研究論文(学術雑誌)  

  • A co-localization model of paired ChIP-seq data using a large ENCODE data set enables comparison of multiple samples 査読

    Kazumitsu Maehara, Jun Odawara, Akihito Harada, Tomohiko Yoshimi, Koji Nagao, Chikashi Obuse, Koichi Akashi, Taro Tachibana, Toshio Sakata, Yasuyuki Ohkawa

    NUCLEIC ACIDS RESEARCH   41 ( 1 )   54 - 62   2013年01月( ISSN:0305-1048

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    掲載種別:研究論文(学術雑誌)  

    Deep sequencing approaches, such as chromatin immunoprecipitation by sequencing (ChIP-seq), have been successful in detecting transcription factor-binding sites and histone modification in the whole genome. An approach for comparing two different ChIP-seq data would be beneficial for predicting unknown functions of a factor. We propose a model to represent co-localization of two different ChIP-seq data. We showed that a meaningful overlapping signal and a meaningless background signal can be separated by this model. We applied this model to compare ChIP-seq data of RNA polymerase II C-terminal domain (CTD) serine 2 phosphorylation with a large amount of peak-called data, including ChIP-seq and other deep sequencing data in the Encyclopedia of DNA Elements (ENCODE) project, and then extracted factors that were related to RNA polymerase II CTD serine 2 in HeLa cells. We further analyzed RNA polymerase II CTD serine 7 phosphorylation, of which their function is still unclear in HeLa cells. Our results were characterized by the similarity of localization for transcription factor/histone modification in the ENCODE data set, and this suggests that our model is appropriate for understanding ChIP-seq data for factors where their function is unknown.

    DOI: 10.1093/nar/gks1010

  • Contribution of Leydig and Sertoli Cells to Testosterone Production in Mouse Fetal Testes 査読

    Yuichi Shima, Kanako Miyabayashi, Shogo Haraguchi, Tatsuhiko Arakawa, Hiroyuki Otake, Takashi Baba, Sawako Matsuzaki, Yurina Shishido, Haruhiko Akiyama, Taro Tachibana, Kazuyoshi Tsutsui, Ken-ichirou Morohashi

    MOLECULAR ENDOCRINOLOGY   27 ( 1 )   63 - 73   2013年01月( ISSN:0888-8809

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    掲載種別:研究論文(学術雑誌)  

    Testosterone is a final product of androgenic hormone biosynthesis, and Leydig cells are known to be the primary source of androgens. In the mammalian testis, two distinct populations of Leydig cells, the fetal and the adult Leydig cells, develop sequentially, and these two cell types differ both morphologically and functionally. It is well known that the adult Leydig cells maintain male reproductive function by producing testosterone. However, it has been controversial whether fetal Leydig cells can produce testosterone, and the synthetic pathway of testosterone in the fetal testis is not fully understood. In the present study, we generated transgenic mice in which enhanced green fluorescence protein was expressed under the control of a fetal Leydig cell-specific enhancer of the Ad4BP/SF-1 (Nr5a1) gene. The transgene construct was prepared by mutating the LIM homeodomain transcription factor (LHX9)-binding sequence in the promoter, which abolished promoter activity in the undifferentiated testicular cells. These transgenic mice were used to collect highly pure fetal Leydig cells. Gene expression and steroidogenic enzyme activities in the fetal Leydig cells as well as in the fetal Sertoli cells and adult Leydig cells were analyzed. Our results revealed that the fetal Leydig cells synthesize only androstenedione because they lack expression of Hsd17b3, and fetal Sertoli cells convert androstenedione to testosterone, whereas adult Leydig cells synthesize testosterone by themselves. The current study demonstrated that both Leydig and Sertoli cells are required for testosterone synthesis in the mouse fetal testis. (Molecular Endocrinology 27: 63-73, 2013)

    DOI: 10.1210/me.2012-1256

  • Custom monoclonal antibody production service by University-originated bio-tech venture 査読

    Tachibana Taro

    JOURNAL OF PHYSIOLOGICAL SCIENCES   63   S105 - S105   2013年( ISSN:1880-6546

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    掲載種別:研究論文(学術雑誌)   共著区分:単著  

  • Custom monoclonal antibody production service by University-originated bio-tech venture 査読

    Tachibana Taro

    JOURNAL OF PHYSIOLOGICAL SCIENCES   63   S105 - S105   2013年( ISSN:1880-6546

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    掲載種別:研究論文(学術雑誌)   共著区分:単著  

  • Custom monoclonal antibody production service by University-originated bio-tech venture 査読

    Taro Tachibana

    JOURNAL OF PHYSIOLOGICAL SCIENCES   63   S105 - S105   2013年( ISSN:1880-6546

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    掲載種別:研究論文(学術雑誌)  

  • Dermokine-beta impairs ERK signaling through direct binding to GRP78 査読

    Higashi Kiyoshi, Hasegawa Minoru, Yokoyama Chikako, Tachibana Taro, Mitsui Shinichi, Saito Koichi

    FEBS LETTERS   586 ( 16 )   2300 - 2305   2012年07月( ISSN:0014-5793

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.febslet.2012.06.022

  • Dermokine-β impairs ERK signaling through direct binding to GRP78.

    Higashi K, Hasegawa M, Yokoyama C, Tachibana T, Mitsui S, Saito K

    FEBS Lett.   586   2300 - 2305   2012年07月

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    掲載種別:研究論文(学術雑誌)  

  • Dermokine-β impairs ERK signaling through direct binding to GRP78. 査読

    Higashi K, Hasegawa M, Yokoyama C, Tachibana T, Mitsui S, Saito K

    FEBS Lett.   586   2300 - 2305   2012年07月

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    掲載種別:研究論文(学術雑誌)  

  • Dermokine-β impairs ERK signaling through direct binding to GRP78. 査読

    Higashi K, Hasegawa M, Yokoyama C, Tachibana T, Mitsui S, Saito K

    FEBS Lett.   586 ( 16 )   2300 - 2305   2012年07月( ISSN:0014-5793

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.febslet.2012.06.022

  • Chd2 interacts with H3.3 to determine myogenic cell fate 査読

    Harada Akihito, Okada Seiji, Konno Daijiro, Odawara Jun, Yoshimi Tomohiko, Yoshimura Saori, Kumamaru Hiromi, Saiwai Hirokazu, Tsubota Toshiaki, Kurumizaka Hitoshi, Akashi Koichi, Tachibana Taro, Imbalzano Anthony N., Ohkawa Yasuyuki

    EMBO JOURNAL   31 ( 13 )   2994 - 3007   2012年06月( ISSN:0261-4189

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/emboj.2012.136

  • Chd2 interacts with H3.3 to determine myogenic cell fate 査読

    Akihito Harada, Seiji Okada, Daijiro Konno, Jun Odawara, Tomohiko Yoshimi, Saori Yoshimura, Hiromi Kumamaru, Hirokazu Saiwai, Toshiaki Tsubota, Hitoshi Kurumizaka, Koichi Akashi, Taro Tachibana, Anthony N. Imbalzano, Yasuyuki Ohkawa

    EMBO JOURNAL   31 ( 13 )   2994 - 3007   2012年06月( ISSN:0261-4189

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    掲載種別:研究論文(学術雑誌)  

    Cell differentiation is mediated by lineage-determining transcription factors. We show that chromodomain helicase DNA-binding domain 2 (Chd2), a SNF2 chromatin remodelling enzyme family member, interacts with MyoD and myogenic gene regulatory sequences to specifically mark these loci via deposition of the histone variant H3.3 prior to cell differentiation. Directed and genome-wide analysis of endogenous H3.3 incorporation demonstrates that knockdown of Chd2 prevents H3.3 deposition at differentiation-dependent, but not housekeeping, genes and inhibits myogenic gene activation. The data indicate that MyoD determines cell fate and facilitates differentiation-dependent gene expression through Chd2-dependent deposition of H3.3 at myogenic loci prior to differentiation. The EMBO Journal (2012) 31, 2994-3007. doi: 10.1038/emboj.2012.136; Published online 8 May 2012

    DOI: 10.1038/emboj.2012.136

  • Chd2 interacts with H3.3 to determine myogenic cell fate.

    Harada A, Okada S, Konno D, Odawara J, Yoshimi T, Yoshimura S, et al.,

    EMBO J.   31   2994 - 3007   2012年05月

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    掲載種別:研究論文(学術雑誌)  

  • Altered expression of dermokine in skin disorders.

    Hasegawa M, Higashi K, Yokoyama C, Yamamoto F, Tachibana T, Matsushita T, Hamaguchi Y, Saito K, Fujimoto M, Takehara K.

    J. Eur. Acad. Dermatol. Venereol.   doi: 10.1111/j.1468-3083.2012.04598.x.   2012年05月

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    掲載種別:研究論文(学術雑誌)  

  • Altered expression of dermokine in skin disorders. 査読

    Hasegawa M, Higashi K, Yokoyama C, Yamamoto F, Tachibana T, Matsushita T, Hamaguchi Y, Saito K, Fujimoto M, Takehara K.

    J. Eur. Acad. Dermatol. Venereol.   doi: 10.1111/j.1468-3083.2012.   2012年05月

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    掲載種別:研究論文(学術雑誌)  

  • Chd2 interacts with H3.3 to determine myogenic cell fate. 査読

    Harada A, Okada S, Konno D, Odawara J, Yoshimi T, Yoshimura S, et al.,

    EMBO J.   31   2994 - 3007   2012年05月

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    掲載種別:研究論文(学術雑誌)  

  • The cytoplasmic tail of heparin-binding EGF-like growth factor regulates bidirectional intracellular trafficking between the plasma membrane and ER 査読

    Hieda Miki, Koizumi Michiko, Higashi Chiduru, Tachibana Taro, Taguchi Tomohiko, Higashiyama Shigeki

    FEBS OPEN BIO   2   339 - 344   2012年( ISSN:2211-5463

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.fob.2012.09.002

  • The cytoplasmic tail of heparin-binding EGF-like growth factor regulates bidirectional intracellular trafficking between the plasma membrane and ER 査読

    Miki Hieda, Michiko Koizumi, Chiduru Higashi, Taro Tachibana, Tomohiko Taguchi, Shigeki Higashiyama

    FEBS OPEN BIO   2   339 - 344   2012年( ISSN:2211-5463

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    掲載種別:研究論文(学術雑誌)  

    Heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF) is synthesized in the ER, transported along the exocytic pathway, and expressed on the plasma membrane as a type I transmembrane protein. Upon extracellular stimulation, HB-EGF, either proHB-EGF or the shed form HB-EGF-CTF, undergoes endocytosis and is then transported retrogradely to the ER. In this study, we showed the essential contribution of the short cytoplasmic tail of HB-EGF (HB-EGF-cyto) to the bidirectional intracellular trafficking between the ER and plasma membrane and revealed several critical amino acids residues that are responsible for internalization from the plasma membrane and ER targeting. We suggest that these anterograde and retrograde sorting signals within HB-EGF-cyto are strictly regulated by protein modification and conformation. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

    DOI: 10.1016/j.fob.2012.09.002

  • Steroidogenic Factor 1 (NR5A1) resides in centrosomes and maintains genomic stability by controlling centrosome homeostasis 査読

    Lai P-Y, Wang C-Y, Chen W-Y, Kao Y-H, Tsai H-M, Tachibana T., Chang W-C, Chung B-C

    CELL DEATH AND DIFFERENTIATION   18 ( 12 )   1836 - 1844   2011年12月( ISSN:1350-9047

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/cdd.2011.54

  • Steroidogenic Factor 1 (NR5A1) resides in centrosomes and maintains genomic stability by controlling centrosome homeostasis.

    Lai PY, Wang CY, Chen WY, Kao YH, Tsai HM, Tachibana T, Chang WC, Chung BC.

    Cell Death Differ.   18 ( 12 )   1836 - 1844   2011年12月

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    掲載種別:研究論文(学術雑誌)  

  • Steroidogenic Factor 1 (NR5A1) resides in centrosomes and maintains genomic stability by controlling centrosome homeostasis. 査読

    Lai PY, Wang CY, Chen WY, Kao YH, Tsai HM, Tachibana T, Chang WC, Chung BC.

    Cell Death Differ.   18 ( 12 )   1836 - 1844   2011年12月

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    掲載種別:研究論文(学術雑誌)  

  • Steroidogenic Factor 1 (NR5A1) resides in centrosomes and maintains genomic stability by controlling centrosome homeostasis 査読

    P-Y Lai, C-Y Wang, W-Y Chen, Y-H Kao, H-M Tsai, T. Tachibana, W-C Chang, B-C Chung

    CELL DEATH AND DIFFERENTIATION   18 ( 12 )   1836 - 1844   2011年12月( ISSN:1350-9047

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    掲載種別:研究論文(学術雑誌)  

    SF-1 (Steroidogenic Factor 1, NR5A1) is a tissue-specific transcription factor critical for the growth, development and differentiation of steroidogenic and a few other endocrine tissues. But how SF-1 regulates cell growth is not entirely clear. Here we found that SF-1 was localized to the centrosome in addition to the nucleus, and SF-1 depletion by shRNA caused centrosome over-duplication, aberrant mitosis and genomic instability, leading to a reduction of cell number. Centrosome amplification defect was rescued by both wild-type SF-1 and transcription-defective SF-1-G35E, suggesting a non-genomic activity of SF-1 involved in centrosome homeostasis. In addition, we identified in SF-1 a centrosome localization signal, whose overexpression led to reduced localization of both SF-1 and gamma-tubulin to the centrosome. Our results uncover a novel role of SF-1 in the control of centrosome homeostasis and genomic stability. Cell Death and Differentiation (2011) 18, 1836-1844; doi:10.1038/cdd.2011.54; published online 13 May 2011

    DOI: 10.1038/cdd.2011.54

  • The classification of mRNA expression levels by the phosphorylation state of RNAPII CTD based on a combined genome-wide approach 査読

    Odawara Jun, Harada Akihito, Yoshimi Tomohiko, Maehara Kazumitsu, Tachibana Taro, Okada Seiji, Akashi Koichi, Ohkawa Yasuyuki

    BMC GENOMICS   12   2011年10月( ISSN:1471-2164

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1186/1471-2164-12-516

  • Cross-talk between distinct nuclear import pathways enables efficient nuclear import of E47 in conjunction with its partner transcription factors 査読

    Mehmood Rashid, Yasuhara Noriko, Fukumoto Masahiro, Oe Souichi, Tachibana Taro, Yoneda Yoshihiro

    MOLECULAR BIOLOGY OF THE CELL   22 ( 19 )   3715 - 3724   2011年10月( ISSN:1059-1524

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1091/mbc.E10-10-0809

  • Cross-talk between distinct nuclear import pathways enables efficient nuclear import of E47 in conjunction with its partner transcription factors 査読

    Rashid Mehmood, Noriko Yasuhara, Masahiro Fukumoto, Souichi Oe, Taro Tachibana, Yoshihiro Yoneda

    MOLECULAR BIOLOGY OF THE CELL   22 ( 19 )   3715 - 3724   2011年10月( ISSN:1059-1524

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    掲載種別:研究論文(学術雑誌)  

    Nuclear import of karyophilic proteins is carried out by a variety of mechanisms. We previously showed that two basic helix-loop-helix proteins, NeuroD1 and E47, synergistically affect each other&apos;s nuclear import. In this study, we dissected the molecular pathways underlying nuclear import of the NeuroD1/E47 heterodimer. In vitro nuclear import assays indicated that importin alpha family members are the major nuclear import receptors for E47. However, inhibition of importin alpha resulted in cytoplasmic retention of E47 that could be rescued by its binding partner, NeuroD1, through heterodimerization. In addition, nuclear import of NeuroD1 was importin alpha independent but importin beta 1 dependent. In primary neurons, localization of endogenous E47 was not affected by importin alpha inhibition, suggesting that neuronal E47 could be imported into the nucleus as a heterodimer with NeuroD1 by using importin beta 1 alone. We also found that E47 had similar nuclear import characteristics in C2C12 cells, where E47 heterodimerized with MyoD, another helix-loop-helix protein, suggesting functional conservation within the same family of transcription factors. Collectively, our data reveal that E47 is imported into the nucleus via multiple pathways, depending on the molecular binding mode, establishing a previously uncharacterized cross-talk between two distinct nuclear import pathways.

    DOI: 10.1091/mbc.E10-10-0809

  • The classification of mRNA expression levels by the phosphorylation state of RNAPII CTD based on a combined genome-wide approach 査読

    Jun Odawara, Akihito Harada, Tomohiko Yoshimi, Kazumitsu Maehara, Taro Tachibana, Seiji Okada, Koichi Akashi, Yasuyuki Ohkawa

    BMC GENOMICS   12   516   2011年10月( ISSN:1471-2164

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    掲載種別:研究論文(学術雑誌)  

    Background: Cellular function is regulated by the balance of stringently regulated amounts of mRNA. Previous reports revealed that RNA polymerase II (RNAPII), which transcribes mRNA, can be classified into the pausing state and the active transcription state according to the phosphorylation state of RPB1, the catalytic subunit of RNAPII. However, genome-wide association between mRNA expression level and the phosphorylation state of RNAPII is unclear. While the functional importance of pausing genes is clear, such as in mouse Embryonic Stem cells for differentiation, understanding this association is critical for distinguishing pausing genes from active transcribing genes in expression profiling data, such as microarrays and RNAseq. Therefore, we examined the correlation between the phosphorylation of RNAPII and mRNA expression levels using a combined analysis by ChIPseq and RNAseq.
    Results: We first performed a precise quantitative measurement of mRNA by performing an optimized calculation in RNAseq. We then visualized the recruitment of various phosphorylated RNAPIIs, such as Ser2P and Ser5P. A combined analysis using optimized RNAseq and ChIPseq for phosphorylated RNAPII revealed that mRNA levels correlate with the various phosphorylation states of RNAPII.
    Conclusions: We demonstrated that the amount of mRNA is precisely reflected by the phased phosphorylation of Ser2 and Ser5. In particular, even the most "pausing" genes, for which only Ser5 is phosphorylated, were detectable at a certain level of mRNA. Our analysis indicated that the complexity of quantitative regulation of mRNA levels could be classified into three categories according to the phosphorylation state of RNAPII.

    DOI: 10.1186/1471-2164-12-516

  • Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibodies recognizing the deamidated alpha subunit of the heterotrimeric GTPase G(q) 査読

    Kamitani Shigeki, Ao Shinpei, Toshima Hirono, Tachibana Taro, Hashimoto Makiko, Kitadokoro Kengo, Fukui-Miyazaki Aya, Abe Hiroyuki, Horiguchi Yasuhiko

    FEBS JOURNAL   278 ( 15 )   2702 - 2712   2011年08月( ISSN:1742-464X

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/j.1742-4658.2011.08197.x

  • Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibodies recognizing the deamidated alpha subunit of the heterotrimeric GTPase G(q) 査読

    Shigeki Kamitani, Shinpei Ao, Hirono Toshima, Taro Tachibana, Makiko Hashimoto, Kengo Kitadokoro, Aya Fukui-Miyazaki, Hiroyuki Abe, Yasuhiko Horiguchi

    FEBS JOURNAL   278 ( 15 )   2702 - 2712   2011年08月( ISSN:1742-464X

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    掲載種別:研究論文(学術雑誌)  

    Pasteurella multocida toxin (PMT) is a virulence factor responsible for the pathogenesis of some Pasteurellosis. PMT exerts its toxic effects through the activation of heterotrimeric GTPase (G(q), G(12/13) and G(i))-dependent pathways, by deamidating a glutamine residue in the alpha subunit of these GTPases. However, the enzymatic characteristics of PMT are yet to be analyzed in detail because the deamidation has only been observed in cell-based assays. In the present study, we developed rat monoclonal antibodies, specifically recognizing the deamidated G alpha(q), to detect the actions of PMT by immunological techniques such as western blotting. Using the monoclonal antibodies, we found that the toxin deamidated G alpha(q) only under reducing conditions. The C-terminal region of PMT, C-PMT, was more active than the full-length PMT. The C3 domain possessing the enzyme core catalyzed the deamidation in vitro without any other domains. These results not only support previous observations on toxicity, but also provide insights into the enzymatic nature of PMT. In addition, we present several lines of evidence that G alpha(11), as well as G alpha(q), could be a substrate for PMT.

    DOI: 10.1111/j.1742-4658.2011.08197.x

  • Mitochondrial Prohibitins and Septin 9 Are Implicated in the Onset of Rat Hepatocarcinogenesis 査読

    Kakehashi Anna, Ishii Naomi, Shibata Takeshi, Wei Min, Okazaki Etsuko, Tachibana Taro, Fukushima Shoji, Wanibuchi Hideki

    TOXICOLOGICAL SCIENCES   119 ( 1 )   61 - 72   2011年01月( ISSN:1096-6080

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/toxsci/kfq307

  • Mitochondrial prohibitins and septin 9 are implicated in the onset of rat hepatocarcinogenesis. 査読

    Kakehashi A, Ishii N, Shibata T, Wei M, Okazaki E, Tachibana T, Fukushima S, Wanibuchi H

    Toxicological sciences : an official journal of the Society of Toxicology   119 ( 1 )   61 - 72   2011年01月( ISSN:1096-6080

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/toxsci/kfq307

    PubMed

  • Mitochondrial prohibitins and septin 9 are implicated in the onset of rat hepatocarcinogenesis.

    Kakehashi A, Ishii N, Shibata T, Wei M, Okazaki E, Tachibana T, Fukushima S, Wanibuchi H.

    Toxicol Sci.   119   61 - 72   2011年

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    掲載種別:研究論文(学術雑誌)  

  • Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibody recognizing the deamidated alpha subunit of the heterotrimeric GTPase Gq.

    Kamitani,S., Ao, S., Toshima, H., Tachibana, T., et al.,

    FEBS Journal   278   2702 - 2712   2011年

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    掲載種別:研究論文(学術雑誌)  

  • Cross talk between distinct nuclear import pathways enables efficient nuclear import of E47 in conjunction with its partner transcription factors.

    Mehmood, R., Yasuhara, N., Fukumoto, M., Oe, S., Tachibana, T. and Yoneda, Y.

    Mol.Biol.Cell   22   3715 - 3724   2011年

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    掲載種別:研究論文(学術雑誌)  

  • The classification of mRNA expression levels by the phosphorylation state of RNAPII CTD based on a combined genome-wide approach.

    Odawara J, Harada A, Yoshimi T, Maehara K, Tachibana T, Okada S, Akashi K, Ohkawa Y.

    BMC Genomics   12   516   2011年

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    掲載種別:研究論文(学術雑誌)  

  • Cross talk between distinct nuclear import pathways enables efficient nuclear import of E47 in conjunction with its partner transcription factors. 査読

    Mehmood, R., Yasuhara, N., Fukumoto, M., Oe, S., Tachibana, T. and Yoneda, Y.

    Mol.Biol.Cell   22   3715 - 3724   2011年

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    掲載種別:研究論文(学術雑誌)  

  • The classification of mRNA expression levels by the phosphorylation state of RNAPII CTD based on a combined genome-wide approach. 査読

    Odawara J, Harada A, Yoshimi T, Maehara K, Tachibana T, Okada S, Akashi K, Ohkawa Y.

    BMC Genomics   12   516   2011年

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    掲載種別:研究論文(学術雑誌)  

  • Mitochondrial prohibitins and septin 9 are implicated in the onset of rat hepatocarcinogenesis. 査読

    Kakehashi A, Ishii N, Shibata T, Wei M, Okazaki E, Tachibana T, Fukushima S, Wanibuchi H.

    Toxicol Sci.   119   61 - 72   2011年

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    掲載種別:研究論文(学術雑誌)  

  • Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibody recognizing the deamidated alpha subunit of the heterotrimeric GTPase Gq. 査読

    Kamitani,S., Ao, S., Toshima, H., Tachibana, T., et al.,

    FEBS Journal   278   2702 - 2712   2011年

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    掲載種別:研究論文(学術雑誌)  

  • Specific Monoclonal Antibody Against the Nuclear Pore Complex Protein, Nup96 査読

    Mizuguchi Chiaki, Oka Masahiro, Moriyama Tetsuji, Tachibana Taro, Yoneda Yoshihiro

    HYBRIDOMA   29 ( 6 )   551 - 553   2010年12月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2010.0065

  • Specific Monoclonal Antibody Against the Nuclear Pore Complex Protein, Nup96 査読

    Chiaki Mizuguchi, Masahiro Oka, Tetsuji Moriyama, Taro Tachibana, Yoshihiro Yoneda

    HYBRIDOMA   29 ( 6 )   551 - 553   2010年12月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    Nup96 is a component of the Nup107-160 complex, the largest subunit of the nuclear pore complex. Nup96 is generated as a precursor protein with Nup98. However, the mechanism by which Nup96 contributes to cell function is not clear. We report here on the preparation of a monoclonal antibody (MAb) directed against mouse Nup96. The antibody was produced by the hybridization of mouse myeloma cells with lymph node cells from an immunized rat. The antibody, MAb 4H5, specifically recognized Nup96, as evidenced by immunoblotting using the whole cell lysates. In immunostaining using MAb 4H5, a nuclear rim staining pattern was observed. This antibody will be useful in immunoblotting and immunolocalization experiments, as well as further analyses of the biological function and cellular dynamics of this protein.

    DOI: 10.1089/hyb.2010.0065

  • The Mobile FG Nucleoporin Nup98 Is a Cofactor for Crm1-dependent Protein Export 査読

    Oka Masahiro, Asally Munehiro, Yasuda Yoshinari, Ogawa Yutaka, Tachibana Taro, Yoneda Yoshihiro

    MOLECULAR BIOLOGY OF THE CELL   21 ( 11 )   1885 - 1896   2010年06月( ISSN:1059-1524

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1091/mbc.E09-12-1041

  • The mobile FG nucleoporin Nup98 is a cofactor for Crm1-dependent protein export. 査読

    Oka M, Asally M, Yasuda Y, Ogawa Y, Tachibana T, Yoneda Y

    Molecular biology of the cell   21 ( 11 )   1885 - 96   2010年06月( ISSN:1059-1524

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1091/mbc.e09-12-1041

    PubMed

  • Monoclonal Antibody Specific for Dhx9/NDHII/RHA 査読

    Kotani Manato, Harada Akihito, Odawara Jun, Azuma Masayuki, Okada Seiji, Nishiyama Yuko, Nakamura Mako, Tachibana Taro, Ohkawa Yasuyuki

    HYBRIDOMA   29 ( 3 )   259 - 261   2010年06月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2009.0107

  • Rat Monoclonal Antibody Specific for the Chromatin Remodeling Factor, CHD1 査読

    Yoshimura Saori, Harada Akihito, Odawara Jun, Azuma Masayuki, Okada Seiji, Nakamura Mako, Ohkawa Yasuyuki, Tachibana Taro

    HYBRIDOMA   29 ( 3 )   237 - 240   2010年06月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2009.0106

  • Rat Monoclonal Antibody Specific for MyoD 査読

    Harada Akihito, Ohkawa Yasuyuki, Ao Shinpei, Odawara Jun, Okada Seiji, Azuma Masayuki, Nishiyama Yuko, Nakamura Mako, Tachibana Taro

    HYBRIDOMA   29 ( 3 )   255 - 258   2010年06月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2009.0117

  • Live imaging system for visualizing nuclear pore complex (NPC) formation during interphase in mammalian cells 査読

    Iino Haruki, Maeshima Kazuhiro, Nakatomi Reiko, Kose Shingo, Hashikawa Tsutomu, Tachibana Taro, Imamoto Naoko

    GENES TO CELLS   15 ( 6 )   647 - 660   2010年06月( ISSN:1356-9597

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1111/j.1365-2443.2010.01406.x

  • Live imaging system for visualizing nuclear pore complex (NPC) formation during interphase in mammalian cells 査読

    Haruki Iino, Kazuhiro Maeshima, Reiko Nakatomi, Shingo Kose, Tsutomu Hashikawa, Taro Tachibana, Naoko Imamoto

    GENES TO CELLS   15 ( 6 )   647 - 660   2010年06月( ISSN:1356-9597

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    掲載種別:研究論文(学術雑誌)  

    Nuclear pore complexes (NPCs) are &apos;supramolecular complexes&apos; on the nuclear envelope assembled from multiple copies of approximately 30 different proteins called nucleoporins (Nups) that provide aqueous channels for nucleocytoplasmic transport during interphase. Although the structural aspects of NPCs have been characterized in detail, NPC formation and its regulation, especially during interphase, are poorly understood. In this study, using the temperature-sensitive RCC1 mutant tsBN2, a baby hamster kidney 21 cell line, we found that a lack of RCC1 activity inhibited NPC formation during interphase, suggesting that RanGTP is required for NPC formation during interphase in mammalian cells. Utilizing the reversible RCC1 activity in tsBN2 cells, we established a live-cell system that allows for the inhibition or initiation of NPC formation by changes in temperature. Our system enables the examination of NPC formation during interphase in living cells. As a lack of RCC1 decreased some Nups containing unstructured phenylalanine-glycine repeats in the NPC structure, we propose that RCC1 is also involved in maintaining NPC integrity during interphase in mammalian cells.

    DOI: 10.1111/j.1365-2443.2010.01406.x

  • Rat monoclonal antibody specific for the chromatin remodeling factor, CHD1. 査読

    Yoshimura S, Harada A, Odawara J, Azuma M, Okada S, Nakamura M, Ohkawa Y, Tachibana T

    Hybridoma (2005)   29 ( 3 )   237 - 240   2010年06月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2009.0106

    PubMed

  • Rat Monoclonal Antibody Specific for MyoD 査読

    Akihito Harada, Yasuyuki Ohkawa, Shinpei Ao, Jun Odawara, Seiji Okada, Masayuki Azuma, Yuko Nishiyama, Mako Nakamura, Taro Tachibana

    HYBRIDOMA   29 ( 3 )   255 - 258   2010年06月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    Myogenic determination 1 (MyoD) is a myogenic regulatory factor (MRF) possessing a basic domain and a helix-loop-helix domain. MRFs play a critical role in myoblast fate and terminal differentiation. MyoD is a transcriptional factor that induces transcription by binding with gene regulatory factors expressed in skeletal muscle. As a master gene, MyoD also determines skeletal muscle differentiation. In this study, we established a monoclonal antibody specific for MyoD using the rat medial iliac lymph node method. Immunoblot analysis revealed that our monoclonal antibody against MyoD could identify full-length MyoD. Moreover, immunocytochemical staining revealed a change in the expression of MyoD at the skeletal muscle differentiation stage. This monoclonal antibody against MyoD allows for further studies to elucidate the mechanism by which MyoD influences skeletal muscle differentiation.

    DOI: 10.1089/hyb.2009.0117

  • Monoclonal antibody specific for Dhx9/NDHII/RHA. 査読

    Kotani M, Harada A, Odawara J, Azuma M, Okada S, Nishiyama Y, Nakamura M, Tachibana T, Ohkawa Y

    Hybridoma (2005)   29 ( 3 )   259 - 261   2010年06月( ISSN:1554-0014

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2009.0107

    PubMed

  • Rat Monoclonal Antibody Specific for Septin 9 査読

    Tachibana Taro, Okazaki Etsuko, Yoshimi Tomohiko, Azuma Masayuki, Kakehashi Anna, Wanibuchi Hideki

    HYBRIDOMA   29 ( 2 )   169 - 171   2010年04月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2009.0092

  • Generation of a Rat Monoclonal Antibody Specific for Chd2 査読

    Harada Akihito, Yoshimura Saori, Odawara Jun, Azuma Masayuki, Okada Seiji, Nakamura Mako, Tachibana Taro, Ohkawa Yasuyuki

    HYBRIDOMA   29 ( 2 )   173 - 177   2010年04月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2009.0090

  • Generation of a Rat Monoclonal Antibody Specific for Chd2 査読

    Akihito Harada, Saori Yoshimura, Jun Odawara, Masayuki Azuma, Seiji Okada, Mako Nakamura, Taro Tachibana, Yasuyuki Ohkawa

    HYBRIDOMA   29 ( 2 )   173 - 177   2010年04月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    CHD2 is a member of the CHD family that contains chromodomain, helicase domain as well as DNA-binding domain. The CHD family is involved in gene expression and transcription by ATP-dependent chromatin remodeling. Analysis of mutant mouse revealed that CHD2 is involved in development as well as hematopoiesis, which suggests the involvement of CHD2 in gene expression. However, CHD2 has not yet been analyzed biochemically as there is no specific antibody against it. Here, we report on the establishment of specific monoclonal antibody (MAb) against CHD2 utilizing a rat medial iliac lymph node method. Through cell immunostaining utilizing established MAb to CHD2, we confirmed that CHD2 was localized in euchromatin. Additionally, IP-Western revealed that the expression level of full-length CHD2 did not change during the differentiation stage. Additionally, a specific signal was confirmed around 95 kDa at the undifferentiated stage. This clearly indicated that CHD2 was involved in specific gene expression at this stage. Thus, this antibody can contribute to elucidating the function of CHD2 in cell expression.

    DOI: 10.1089/hyb.2009.0090

  • Rat monoclonal antibody specific for septin 9. 査読

    Tachibana T, Okazaki E, Yoshimi T, Azuma M, Kakehashi A, Wanibuchi H

    Hybridoma (2005)   29 ( 2 )   169 - 171   2010年04月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2009.0092

    PubMed

  • A Rat Monoclonal Antibody Against the Chromatin Remodeling Factor CHD5 査読

    Yoshimura Saori, Yoshimi Tomohiko, Ohkawa Yasuyuki, Azuma Masayuki, Tachibana Taro

    HYBRIDOMA   29 ( 1 )   63 - 66   2010年02月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2009.0069

  • Production and Characterization of Monoclonal Antibodies to Mouse Germ Cells 査読

    yokoyama Chikako, Katoh-Fukui Yuko, Morohashi Ken-ichirou, Konno Daijiro, Azuma Masayuki, Tachibana Taro

    HYBRIDOMA   29 ( 1 )   53 - 57   2010年02月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2009.0101

  • Monoclonal Antibody 3G4 Anti-Brg1 査読

    Tachibana Taro

    HYBRIDOMA   29 ( 1 )   81 - 81   2010年02月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)   共著区分:単著  

    DOI: 10.1089/hyb.2009.0078.MAb

  • A rat monoclonal antibody against the chromatin remodeling factor CHD5. 査読

    Yoshimura S, Yoshimi T, Ohkawa Y, Azuma M, Tachibana T

    Hybridoma (2005)   29 ( 1 )   63 - 66   2010年02月( ISSN:1554-0014

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2009.0069

    PubMed

  • Production and characterization of monoclonal antibodies to mouse germ cells. 査読

    Yokoyama C, Katoh-Fukui Y, Morohashi K, Konno D, Azuma M, Tachibana T

    Hybridoma (2005)   29 ( 1 )   53 - 57   2010年02月( ISSN:1554-0014

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2009.0101

    PubMed

  • Monoclonal Antibody 3G4 Anti-Brg1 査読

    Taro Tachibana

    HYBRIDOMA   29 ( 1 )   81 - 81   2010年02月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2009.0078.MAb

  • Cytokeratin 8/18 as a new marker of mouse liver preneoplastic lesions 査読

    Kakehashi Anna, Kato Ayumi, Inoue Masayo, Ishii Naomi, Okazaki Etsuko, Wei Min, Tachibana Taro, Wanibuchi Hideki

    TOXICOLOGY AND APPLIED PHARMACOLOGY   242 ( 1 )   47 - 55   2010年01月( ISSN:0041-008X

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.taap.2009.09.013

  • Dissecting differential gene expression within the circadian neuronal circuit of Drosophila 査読

    Nagoshi Emi, Sugino Ken, Kula Ela, Okazaki Etsuko, Tachibana Taro, Nelson Sacha, Rosbash Michael

    NATURE NEUROSCIENCE   13 ( 1 )   60 - U220   2010年01月( ISSN:1097-6256

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/nn.2451

  • Cytokeratin 8/18 as a new marker of mouse liver preneoplastic lesions. 査読

    Kakehashi A, Kato A, Inoue M, Ishii N, Okazaki E, Wei M, Tachibana T, Wanibuchi H

    Toxicology and applied pharmacology   242 ( 1 )   47 - 55   2010年01月( ISSN:0041-008X

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.taap.2009.09.013

    PubMed

  • Molecular characterization and expression of the low-density lipoprotein receptor-related protein-10, a new member of the LDLR gene family 査読

    Young-Hee Jeong, Kayoko Ishikawa, Yoshimi Someya, Akemi Hosoda, Tomohiko Yoshimi, Chikako Yokoyama, Sumiko Kiryu-Seo, Man-Jong Kang, Taro Tchibana, Hiroshi Kiyama, Tomoe Fukumura, Dong-Ho Kim, Shigeru Saeki

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   391 ( 1 )   1110 - 1115   2010年01月( ISSN:0006-291X

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    掲載種別:研究論文(学術雑誌)  

    We report the characterization of a new member of the low-density lipoprotein receptor (LDLR) gene family designated LRP10. Human LRP10 cDNA encodes a 1905 amino acid type I membrane protein consisting of five functional domains characteristic of the LDLR gene family. CHO-ldlA7 cells transfected with human LRP10 cDNA bound LDLR-associated protein, but not beta-VLDL and HDL. Human LRP10 transcripts were primarily found in the brain, muscle and heart. in situ hybridization of the rat brain showed that the transcripts were intensely present in the cerebral cortex, hippocampus, choroid plexus, ependyma and granular layer. In the developing rat brain, transcript levels gradually increased from postnatal day 1 to 20. Immunofluorescence analysis indicated that LRP10 was observed in the ventricular zone of the embryonic day 14.5 mouse cerebral cortex. The present studies suggest that LRP10 may play a significant role in the brain physiology other than lipoprotein metabolism. (C) 2009 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2009.12.033

  • Dissecting differential gene expression within the circadian neuronal circuit of Drosophila 査読

    Emi Nagoshi, Ken Sugino, Ela Kula, Etsuko Okazaki, Taro Tachibana, Sacha Nelson, Michael Rosbash

    NATURE NEUROSCIENCE   13 ( 1 )   60 - U220   2010年01月( ISSN:1097-6256

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    掲載種別:研究論文(学術雑誌)  

    Behavioral circadian rhythms are controlled by a neuronal circuit consisting of diverse neuronal subgroups. To understand the molecular mechanisms underlying the roles of neuronal subgroups within the Drosophila circadian circuit, we used cell-type specific gene-expression profiling and identified a large number of genes specifically expressed in all clock neurons or in two important subgroups. Moreover, we identified and characterized two circadian genes, which are expressed specifically in subsets of clock cells and affect different aspects of rhythms. The transcription factor Fer2 is expressed in ventral lateral neurons; it is required for the specification of lateral neurons and therefore their ability to drive locomotor rhythms. The Drosophila melanogaster homolog of the vertebrate circadian gene nocturnin is expressed in a subset of dorsal neurons and mediates the circadian light response. The approach should also enable the molecular dissection of many different Drosophila neuronal circuits.

    DOI: 10.1038/nn.2451

  • Rat Monoclonal Antibody Specific for Septin 9.

    Tachibana, T., Okazaki, E., Yoshimi, T., Azuma, M., Kakehashi A. and Wanibuchi, H.

    Hybridoma   29   169 - 171   2010年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Rat monoclonal antibody specific for the chromatin remodeling factor CHD1.

    Yoshimura, S., Harada, A., Odawara, J., Azuma, M., Okada, S., Nakamura, M., Ohkawa, Y. and Tachibana, T.

    Hybridoma   29   237 - 240   2010年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Production and Characterization of Monoclonal Antibodies to Mouse Germ Cells.

    Yokoyama, C., Katoh-Fukui, Y., Morohashi, K., Konno, D., Azuma, M. and Tachibana, T.

    Hybridoma   29   53 - 57   2010年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Production of a Rat Monoclonal Antibody Specific for Dhx9/NDHII/RHA.

    Kotani, M., Harada, A., Odawara, J., Azuma, M., Okada, S., Nishiyama, Y., Nakamura, M., Tachibana, T. and Ohkawa, Y.

    Hybridoma   29   259 - 261   2010年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Generation of a Rat Monoclonal Antibody Specific for MyoD.

    Harada, A., Ohkawa, Y., Ao, S., Odawara, J., Okada, S., Azuma, M., Nishiyama, Y., Nakamura, M., and Tachibana, T.

    Hybridoma   29   255 - 258   2010年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Molecular characterization and expression of the low-density lipoprotein receptor-related protein-10, a new member of the LDLR gene family.

    Jeong, Y-H., Ishikawa, K., Someya, Y., Hosoda, A., Yoshimi, T., Yokoyama, C., et al.,

    Biochem. Biophys. Res. Commun.   391   1110 - 1115   2010年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Cytokeratin 8/18 as a new marker of mouse liver preneoplastic lesions.

    Kakehashi,A., Kato, A., Inoue, M., Ishii, N., Okazaki, E., Wei, M., Tachibana, T., and Wanibuchi, H.

    Toxicol. Appl. Pharmacol.   242   47 - 55   2010年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • The mobile FG nucleoporin Nup98 is a cofactor for Crm1-dependent protein export.

    Oka, M., Asally,M., Yasuda, Y., Ogawa, Y., Tachibana,T. and Yoneda,Y.

    Mol.Biol.Cell   21   1885 - 1896   2010年

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    掲載種別:研究論文(学術雑誌)  

  • Live imaging system for visualizing nuclear pore complex (NPC) formation during interphase in mammalian cells.

    Iino H, Maeshima K, Nakatomi R, Kose S, Hashikawa T, Tachibana T, Imamoto N.

    Genes Cells   15   647 - 660   2010年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Specific Monoclonal Antibody Against the Nuclear Pore Complex Protein, Nup96.

    Mizuguchi C, Oka M, Moriyama T, Tachibana T, Yoneda Y.

    Hybridoma   29   551 - 553   2010年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Cytokeratin 8/18 as a new marker of mouse liver preneoplastic lesions. 査読

    Kakehashi,A., Kato, A., Inoue, M., Ishii, N., Okazaki, E., Wei, M., Tachibana, T., and Wanibuchi, H.

    Toxicol. Appl. Pharmacol.   242   47 - 55   2010年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • The mobile FG nucleoporin Nup98 is a cofactor for Crm1-dependent protein export. 査読

    Oka, M., Asally,M., Yasuda, Y., Ogawa, Y., Tachibana,T. and Yoneda,Y.

    Mol.Biol.Cell   21   1885 - 1896   2010年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Specific Monoclonal Antibody Against the Nuclear Pore Complex Protein, Nup96. 査読

    Mizuguchi C, Oka M, Moriyama T, Tachibana T, Yoneda Y.

    Hybridoma   29   551 - 553   2010年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Rat monoclonal antibody specific for the chromatin remodeling factor CHD1. 査読

    Yoshimura, S., Harada, A., Odawara, J., Azuma, M., Okada, S., Nakamura, M., Ohkawa, Y. and Tachibana, T.

    Hybridoma   29   237 - 240   2010年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Rat Monoclonal Antibody Specific for Septin 9. 査読

    Tachibana, T., Okazaki, E., Yoshimi, T., Azuma, M., Kakehashi A. and Wanibuchi, H.

    Hybridoma   29   169 - 171   2010年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Production of a Rat Monoclonal Antibody Specific for Dhx9/NDHII/RHA. 査読

    Kotani, M., Harada, A., Odawara, J., Azuma, M., Okada, S., Nishiyama, Y., Nakamura, M., Tachibana, T. and Ohkawa, Y.

    Hybridoma   29   259 - 261   2010年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Production and Characterization of Monoclonal Antibodies to Mouse Germ Cells. 査読

    Yokoyama, C., Katoh-Fukui, Y., Morohashi, K., Konno, D., Azuma, M. and Tachibana, T.

    Hybridoma   29   53 - 57   2010年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Molecular characterization and expression of the low-density lipoprotein receptor-related protein-10, a new member of the LDLR gene family. 査読

    Jeong, Y-H., Ishikawa, K., Someya, Y., Hosoda, A., Yoshimi, T., Yokoyama, C., et al.,

    Biochem. Biophys. Res. Commun.   391   1110 - 1115   2010年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Live imaging system for visualizing nuclear pore complex (NPC) formation during interphase in mammalian cells. 査読

    Iino H, Maeshima K, Nakatomi R, Kose S, Hashikawa T, Tachibana T, Imamoto N.

    Genes Cells   15   647 - 660   2010年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Generation of a Rat Monoclonal Antibody Specific for MyoD. 査読

    Harada, A., Ohkawa, Y., Ao, S., Odawara, J., Okada, S., Azuma, M., Nishiyama, Y., Nakamura, M., and Tachibana, T.

    Hybridoma   29   255 - 258   2010年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • 酵母を用いた小型グルコース燃料電池の性能向上 査読

    高野 晃輔, 脇坂 知行, 立花 太郎, 東 雅之

    化学工学会 研究発表講演要旨集   2010   677 - 677   2010年

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.11491/scej.2010f.0.677.0

    CiNii Article

  • Production of a Rat Monoclonal Antibody Against Brg1 査読

    Ohkawa Yasuyuki, Harada Akihito, Nakamura Mako, Yoshimura Saori, Tachibana Taro

    HYBRIDOMA   28 ( 6 )   463 - 466   2009年12月( ISSN:1554-0014

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2009.0041

  • Generation of A Rat Monoclonal Antibody Specific for Glyoxalase I 査読

    Nakadate Yusuke, Mizuguchi Chiaki, Azuma Masayuki, Tachibana Taro

    HYBRIDOMA   28 ( 6 )   447 - 450   2009年12月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2009.0048

  • Monoclonal Antibody 6F10 Anti-glyoxalase I (GLO1) 査読

    Tachibana Taro

    HYBRIDOMA   28 ( 6 )   467 - 467   2009年12月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)   共著区分:単著  

    DOI: 10.1089/hyb.2009.0063.MAb

  • Generation of a rat monoclonal antibody specific for glyoxalase I. 査読

    Nakadate Y, Mizuguchi C, Azuma M, Tachibana T

    Hybridoma (2005)   28 ( 6 )   447 - 450   2009年12月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2009.0048

    PubMed

  • Production of a Rat Monoclonal Antibody Against Brg1 査読

    Yasuyuki Ohkawa, Akihito Harada, Mako Nakamura, Saori Yoshimura, Taro Tachibana

    HYBRIDOMA   28 ( 6 )   463 - 466   2009年12月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    Brm-related gene-1 (Brg1) is a catalytic subunit of the SWI/SNF chromatin remodeling enzyme complex that has ATPase activity. This complex facilitates chromatin remodeling for gene expression by utilizing energy for ATP hydrolysis. It is well known that the SWI/SNF chromatin remodeling enzyme complex is essential for cell differentiation, cell cycle regulation, and embryogenesis. Here we report the establishment of a hybridoma cell line for producing an antibody against Brg1 subunit by the rat medial iliac lymph node method. Immunoblot analysis showed that our antibody can specifically recognize Brg1. It was revealed by immunocytochemistry that Brg1 is located in euchromatin of C2C12 myoblast nuclei. These data suggested this antibody is useful for analyzing molecular function of Brg1 protein in cells.

    DOI: 10.1089/hyb.2009.0041

  • Monoclonal Antibody 6F10 Anti-glyoxalase I (GLO1) 査読

    Taro Tachibana

    HYBRIDOMA   28 ( 6 )   467 - 467   2009年12月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2009.0063.MAb

  • FM-P34 Glucose fuel battery powered by yeast-analysis of the battery for high performance(Section VIII Fermentation and Microbial Technology) 査読

    Azuma Masayuki, Ito Yuichi, Takano Kosuke, Tachibana Taro, Koyama Shinji, Takada Yogo, Wakisaka Tomoyuki

    公益社団法人日本生物工学会 Journal of bioscience and bioengineering   108 ( 1 )   S129 - S130   2009年11月( ISSN:13891723

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    掲載種別:研究論文(学術雑誌)  

    CiNii Article

  • Glucose fuel battery powered by yeast-analysis of the battery for high performance 査読

    Azuma Masayuki, Ito Yuichi, Takano Kosuke, Tachibana Taro, Koyama Shinji, Takada Yogo, Wakisaka Tomoyuki

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   108   S129 - S130   2009年11月( ISSN:1389-1723

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.jbiosc.2009.08.378

  • FM-P34 Glucose fuel battery powered by yeast-analysis of the battery for high performance(Section VIII Fermentation and Microbial Technology) 査読

    Azuma Masayuki, Ito Yuichi, Takano Kosuke, Tachibana Taro, Koyama Shinji, Takada Yogo, Wakisaka Tomoyuki

    Journal of bioscience and bioengineering   108 ( 1 )   S129 - S130   2009年11月( ISSN:13891723

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    掲載種別:研究論文(学術雑誌)  

    CiNii Article

  • Glucose fuel battery powered by yeast-analysis of the battery for high performance 査読

    Masayuki Azuma, Yuichi Ito, Kosuke Takano, Taro Tachibana, Shinji Koyama, Yogo Takada, Tomoyuki Wakisaka

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   108   S129 - S130   2009年11月( ISSN:1389-1723

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.jbiosc.2009.08.378

  • Generation of Rat Monoclonal Antibodies Specific for Ad4BP/SF-1 査読

    Yokoyama Chikako, Komatsu Tomoko, Ogawa Hidesato, Morohashi Ken-ichirou, Azuma Masayuki, Tachibana Taro

    HYBRIDOMA   28 ( 2 )   113 - 119   2009年04月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2008.0084

  • MAbs 1B1, 7D5, and 8G2 Anti-Ad4BP/SF-1 査読

    Tachibana Taro

    HYBRIDOMA   28 ( 2 )   151 - 151   2009年04月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)   共著区分:単著  

    DOI: 10.1089/hyb.2008.0096.MAb

  • Generation of rat monoclonal antibodies specific for Ad4BP/SF-1.

    Yokoyama C, Komatsu T, Ogawa H, Morohashi K, Azuma M, Tachibana T

    Hybridoma (2005)   28 ( 2 )   113 - 119   2009年04月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2008.0084

    PubMed

  • MAbs 1B1, 7D5, and 8G2 Anti-Ad4BP/SF-1 査読

    Taro Tachibana

    HYBRIDOMA   28 ( 2 )   151 - 151   2009年04月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2008.0096.MAb

  • The formation of argpyrimidine, a methylglyoxal-arginine adduct, in the nucleus of neural cells 査読

    Nakadate Yusuke, Uchida Koji, Shikata Keiji, Yoshimura Saori, Azuma Masayuki, Hirata Tatsumi, Konishi Hiroyuki, Kiyama Hiroshi, Tachibana Taro

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   378 ( 2 )   209 - 212   2009年01月( ISSN:0006-291X

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.bbrc.2008.11.028

  • The formation of argpyrimidine, a methylglyoxal-arginine adduct, in the nucleus of neural cells. 査読

    Nakadate Y, Uchida K, Shikata K, Yoshimura S, Azuma M, Hirata T, Konishi H, Kiyama H, Tachibana T

    Biochemical and biophysical research communications   378 ( 2 )   209 - 212   2009年01月( ISSN:0006-291X

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.bbrc.2008.11.028

    PubMed

  • The formation of argpyrimidine, a methylglyoxal-arginine adduct, in the nucleus of neural cells.

    Nakadate, Y., Uchida, K., Shikata, K., Yoshimura, S., Azuma, M., Hirata, T., Konishi, H., Kiyama, H. and Tachibana, T

    Biochem Biophys Res Commun.   378   209 - 212   2009年

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    掲載種別:研究論文(学術雑誌)  

  • Generation of Rat Monoclonal Antibodies Specific for Ad4BP/SF-1.

    Yokoyama, C., Komatsu, T., Ogawa, H., Morohashi, K., Azuma, M. and Tachibana, T.

    Hybridoma   28   113 - 119   2009年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Production of a Rat Monoclonal Antibody against BRG1.

    Ohkawa, Y., Harada, A., Nakamura, M., Yoshimura, S. and Tachibana, T.

    Hybridoma   28   463 - 466   2009年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Generation of a Rat Monoclonal Antibody Specific for Glyoxalase I.

    Nakadate, Y., Mizuguchi, C., Azuma, M. and Tachibana, T.

    Hybridoma   28   447 - 450   2009年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Gene expression dissection of the circadian neuronal circuit of Drosophila identifies novel circadian genes.

    Nagoshi, E., Sugino, K., Kula, E., Okazaki, E., Tachibana, T. and Nelson, S.

    Nature Neuroscience   13   60 - 68   2009年

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    掲載種別:研究論文(学術雑誌)  

  • A Rat Monoclonal Antibody against the Chromatin Remodeling Factor, CHD5.

    Yoshimura, S., Yoshimi, T., Ohkawa, Y., Azuma, M. and Tachibana, T.

    Hybridoma   29   63 - 66   2009年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • A Rat Monoclonal Antibody against the Chromatin Remodeling Factor, CHD5. 査読

    Yoshimura, S., Yoshimi, T., Ohkawa, Y., Azuma, M. and Tachibana, T.

    Hybridoma   29   63 - 66   2009年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • The formation of argpyrimidine, a methylglyoxal-arginine adduct, in the nucleus of neural cells. 査読

    Nakadate, Y., Uchida, K., Shikata, K., Yoshimura, S., Azuma, M., Hirata, T., Konishi, H., Kiyama, H. and Tachibana, T

    Biochem Biophys Res Commun.   378   209 - 212   2009年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Production of a Rat Monoclonal Antibody against BRG1. 査読

    Ohkawa, Y., Harada, A., Nakamura, M., Yoshimura, S. and Tachibana, T.

    Hybridoma   28   463 - 466   2009年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Generation of Rat Monoclonal Antibodies Specific for Ad4BP/SF-1. 査読

    Yokoyama, C., Komatsu, T., Ogawa, H., Morohashi, K., Azuma, M. and Tachibana, T.

    Hybridoma   28   113 - 119   2009年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Generation of a Rat Monoclonal Antibody Specific for Glyoxalase I. 査読

    Nakadate, Y., Mizuguchi, C., Azuma, M. and Tachibana, T.

    Hybridoma   28   447 - 450   2009年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Gene expression dissection of the circadian neuronal circuit of Drosophila identifies novel circadian genes. 査読

    Nagoshi, E., Sugino, K., Kula, E., Okazaki, E., Tachibana, T. and Nelson, S.

    Nature Neuroscience   13   60 - 68   2009年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Gene expression dissection of the circadian neuronal circuit of Drosophila identifies novel circadian genes. 査読

    Nagoshi, E, Sugino, K, Kula, E, Okazaki, E, Tachibana, T, Nelson, S

    Nature Neuroscience   13   60 - 68   2009年

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    掲載種別:研究論文(学術雑誌)  

  • Generation and characterization of a monoclonal antibody against NPI-1 subfamily of importin alpha 査読

    Tachibana Taro, Sakaguchi Naoko, Miyamoto Yoichi, Sekimoto Toshihiro, Yoneda Yoshihiro, Azuma Masayuki

    HYBRIDOMA   27 ( 4 )   285 - 289   2008年08月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2008.0010

  • Monoclonal antibody 2D9 against importin alpha 査読

    Tachibana Taro

    HYBRIDOMA   27 ( 4 )   324 - 324   2008年08月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)   共著区分:単著  

    DOI: 10.1089/hyb.2008.0023.MAb

  • Monoclonal antibody 2D9 against importin alpha 査読

    Taro Tachibana

    HYBRIDOMA   27 ( 4 )   324 - 324   2008年08月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2008.0023.MAb

  • Pituitary homeobox 2 regulates adrenal4 binding protein/steroidogenic factor-1 gene transcription in the pituitary gonadotrope through interaction with the intronic enhancer 査読

    Shima Yuichi, Zubair Mohamad, Komatsu Tomoko, Oka Sanae, Yokoyama Chikako, Tachibana Taro, Hjalt Tord A., Drouin Jacques, Morohashi Ken-ichirou

    MOLECULAR ENDOCRINOLOGY   22 ( 7 )   1633 - 1646   2008年07月( ISSN:0888-8809

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1210/me.2007-0444

  • Pituitary homeobox 2 regulates adrenal4 binding protein/steroidogenic factor-1 gene transcription in the pituitary gonadotrope through interaction with the intronic enhancer 査読

    Yuichi Shima, Mohamad Zubair, Tomoko Komatsu, Sanae Oka, Chikako Yokoyama, Taro Tachibana, Tord A. Hjalt, Jacques Drouin, Ken-ichirou Morohashi

    MOLECULAR ENDOCRINOLOGY   22 ( 7 )   1633 - 1646   2008年07月( ISSN:0888-8809

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    掲載種別:研究論文(学術雑誌)  

    Ad4BP/SF-1 [adrenal4 binding protein/steroidogenic factor-1 (NR5A1)] is a factor important for animal reproduction and endocrine regulation, and its expression is tightly regulated in the gonad, adrenal gland, ventromedial hypothalamic nucleus, and pituitary gonadotrope. Despite its functional significance in the pituitary, the mechanisms underlying pituitary-specific expression of the gene remain to be uncovered. In this study, we demonstrate by transgenic mouse assays that the pituitary gonadotrope-specific enhancer is localized within the sixth intron of the gene. Functionally, the enhancer recapitulates endogenous Ad4BP/SF-1 expression in the fetal Rathke&apos;s pouch to the adult pituitary gonadotrope. Structurally, the enhancer consists of several elements conserved among animal species. Mutational analyses confirmed the significance of these elements for the enhancer function. One of these elements was able to interact both in vitro and in vivo with Pitx2 (pituitary homeobox 2), demonstrating that pituitary homeobox 2 regulates Ad4BP/SF-1 gene transcription in the pituitary gonadotrope via interaction with the gonadotrope-specific enhancer.

    DOI: 10.1210/me.2007-0444

  • Synthesis and Degradation of Acyl Peptide Using Enzyme from Pseudomonas aeruginosa 査読

    Islam Nazneen Naher, Igarashi Koichi, Tachibana Taro, OOSHIMA Hiroshi, AZUMA Masayuki

    公益社団法人日本生物工学会 Journal of bioscience and bioengineering   105 ( 3 )   282 - 287   2008年03月( ISSN:13891723

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    掲載種別:研究論文(学術雑誌)  

    The detailed properties of the enzyme from Pseudomonas aeruginosa, which catalyzes the N-acyl linkage between myristic acid and the N-terminal glycine residue of the octapeptide GNAAAARR-NH_2(PKA) in aqueous solution without ATP and CoA, were studied. The substrate specificity for the acyl peptide in the synthetic reaction was examined, and it was found that at least eight amino acid residues are required for the reaction and that the N-terminal glycine residue is not absolutely essential for the reaction because the activity was detected using the octapeptide that has an N-terminal alanine. The activity was also strongly affected by the amino acid sequence because the activity was very weak in the reaction using GARASVLS-NH_2(HIV-1p17^<gag>). The substrate specificity for fatty acids was also examined. In the reactions using lauric acid and decanoic acid, only slight activities were detected; however, those activities were very small compared with the activity in the reaction using myristic acid. In addition, the degradation of myristoyl PKA by the enzyme was detected, although there are only a few reports on demyristoylation. The optimum pH and temperature of the degradation reaction were consistent with those of the synthetic reaction. The degradation reaction was inhibited by divalent cations.

    CiNii Article

  • Synthesis and Degradation of Acyl Peptide Using Enzyme from Pseudomonas aeruginosa 査読

    Islam Nazneen Naher, Igarashi Koichi, Tachibana Taro, OOSHIMA Hiroshi, AZUMA Masayuki

    Journal of bioscience and bioengineering   105 ( 3 )   282 - 287   2008年03月( ISSN:1389-1723

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    The detailed properties of the enzyme from Pseudomonas aeruginosa, which catalyzes the N-acyl linkage between myristic acid and the N-terminal glycine residue of the octapeptide GNAAAARR-NH_2(PKA) in aqueous solution without ATP and CoA, were studied. The substrate specificity for the acyl peptide in the synthetic reaction was examined, and it was found that at least eight amino acid residues are required for the reaction and that the N-terminal glycine residue is not absolutely essential for the reaction because the activity was detected using the octapeptide that has an N-terminal alanine. The activity was also strongly affected by the amino acid sequence because the activity was very weak in the reaction using GARASVLS-NH_2(HIV-1p17^<gag>). The substrate specificity for fatty acids was also examined. In the reactions using lauric acid and decanoic acid, only slight activities were detected; however, those activities were very small compared with the activity in the reaction using myristic acid. In addition, the degradation of myristoyl PKA by the enzyme was detected, although there are only a few reports on demyristoylation. The optimum pH and temperature of the degradation reaction were consistent with those of the synthetic reaction. The degradation reaction was inhibited by divalent cations.

    CiNii Article

  • Synthesis and degradation of acyl peptide using enzyme from Pseudomonas aeruginosa 査読

    Islam Nazneen Naher, Igarashi Koichi, Tachibana Taro, Ooshima Hiroshi, Azuma Masayuki

    The Society for Biotechnology, Japan JOURNAL OF BIOSCIENCE AND BIOENGINEERING   105 ( 3 )   282 - 287   2008年03月( ISSN:1389-1723

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    掲載種別:研究論文(学術雑誌)  

    The detailed properties of the enzyme from <i>Pseudomonas aeruginosa</i>, which catalyzes the <i>N</i>-acyl linkage between myristic acid and the <i>N</i>-terminal glycine residue of the octapeptide GNAAAARR-NH<sub>2</sub> (PKA) in aqueous solution without ATP and CoA, were studied. The substrate specificity for the acyl peptide in the synthetic reaction was examined, and it was found that at least eight amino acid residues are required for the reaction and that the <i>N</i>-terminal glycine residue is not absolutely essential for the reaction because the activity was detected using the octapeptide that has an <i>N</i>-terminal alanine. The activity was also strongly affected by the amino acid sequence because the activity was very weak in the reaction using GARASVLS-NH<sub>2</sub> (HIV-1p17<sup>gag</sup>). The substrate specificity for fatty acids was also examined. In the reactions using lauric acid and decanoic acid, only slight activities were detected; however, those activities were very small compared with the activity in the reaction using myristic acid. In addition, the degradation of myristoyl PKA by the enzyme was detected, although there are only a few reports on demyristoylation. The optimum pH and temperature of the degradation reaction were consistent with those of the synthetic reaction. The degradation reaction was inhibited by divalent cations.<br>

    DOI: 10.1263/jbb.105.282

    CiNii Article

  • Membrane-anchored growth factor, HB-EGF, on the cell surface targeted to the inner nuclear membrane 査読

    Hieda Miki, Isokane Mayumi, Koizumi Michiko, Higashi Chicluru, Tachibana Taro, Shudou Masachika, Taguchi Tomohiko, Hieda Yohki, Higashiyama Shigeki

    JOURNAL OF CELL BIOLOGY   180 ( 4 )   763 - 769   2008年02月( ISSN:0021-9525

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1083/jcb.200710022

  • Nuclear RNA Export Factor 7 is localized in processing bodies and neuronal RNA granules through interactions with shuttling hnRNPs.

    Katahira, J., Miki, T., Takano, K., Maruhashi, M., Uchikawa, M., Tachibana, T. and Yoneda, Y.

    Nucleic Acids Res.   36   616 - 628   2008年02月

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    掲載種別:研究論文(学術雑誌)  

  • Nuclear RNA export factor 7 is localized in processing bodies and neuronal RNA granules through interactions with shuttling hnRNPs 査読

    Katahira Jun, Miki Takashi, Takano Keizo, Maruhashi Mitsuji, Uchikawa Masanori, Tachibana Taro, Yoneda Yoshihiro

    NUCLEIC ACIDS RESEARCH   36 ( 2 )   616 - 628   2008年02月( ISSN:0305-1048

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1093/nar/gkm556

  • Nuclear RNA Export Factor 7 is localized in processing bodies and neuronal RNA granules through interactions with shuttling hnRNPs. 査読

    Katahira, J., Miki, T., Takano, K., Maruhashi, M., Uchikawa, M., Tachibana, T. and Yoneda, Y.

    Nucleic Acids Res.   36   616 - 628   2008年02月

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    掲載種別:研究論文(学術雑誌)  

  • Nuclear RNA export factor 7 is localized in processing bodies and neuronal RNA granules through interactions with shuttling hnRNPs 査読

    Jun Katahira, Takashi Miki, Keizo Takano, Mitsuji Maruhashi, Masanori Uchikawa, Taro Tachibana, Yoshihiro Yoneda

    NUCLEIC ACIDS RESEARCH   36 ( 2 )   616 - 628   2008年02月( ISSN:0305-1048

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    掲載種別:研究論文(学術雑誌)  

    The nuclear RNA export factor (NXF) family proteins have been implicated in various aspects of post-transcriptional gene expression. This study shows that mouse NXF7 exhibits heterologous localization, i.e. NXF7 associates with translating ribosomes, stress granules (SGs) and processing bodies (P-bodies), the latter two of which are believed to be cytoplasmic sites of storage, degradation and/or sorting of mRNAs. By yeast two-hybrid screening, a series of heterogeneous nuclear ribonucleoproteins (hnRNPs) were identified as possible binding partners for NXF7. Among them, hnRNP A3, which is believed to be involved in translational control and/or cytoplasmic localization of certain mRNAs, formed a stable complex with NXF7 in vitro. Although hnRNP A3 was not associated with translating ribosomes, it was co-localized with NXF7 in P-bodies. After exposing to oxidative stress, NXF7 trans-localized to SGs, whereas hnRNP A3 did not. In differentiated neuroblastoma Neuro2a cells, NXF7 was co-localized with hnRNP A3 in cell body and neurites. The amino terminal half of NXF7, which was required for stable complex formation with hnRNP A3, coincided with the region required for localization in both P-bodies and neuronal RNA granules. These findings suggest that NXF7 plays a role in sorting, transport and/or storage of mRNAs through interactions with hnRNP A3.

    DOI: 10.1093/nar/gkm556

  • Membrane-anchored growth factor, HB-EGF, on the cell surface targeted to the inner nuclear membrane 査読

    Miki Hieda, Mayumi Isokane, Michiko Koizumi, Chicluru Higashi, Taro Tachibana, Masachika Shudou, Tomohiko Taguchi, Yohki Hieda, Shigeki Higashiyama

    JOURNAL OF CELL BIOLOGY   180 ( 4 )   763 - 769   2008年02月( ISSN:0021-9525

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    掲載種別:研究論文(学術雑誌)  

    Heparin-binding EGF-like growth factor (HB-EGF) is synthesized as a type I transmembrane protein (proHB-EGF) and expressed on the cell surface. The ectodomain shedding of proHB-EGF at the extracellular region on the plasma membrane yields a soluble EGF receptor ligand and a transmembrane-cytoplasmic fragment (HB-EGF-CTF). The cytoplasmic domain of proHB-EGF (HB-EGF-cyto) interacts with transcriptional repressors to reverse their repressive activities. However, how HB-EGF-cyto accesses transcriptional repressors is yet unknown. The present study demonstrates that, after exposure to shedding stimuli, both HB-EGF-CTF and unshed proHB-EGF translocate to the nuclear envelope. Immunoelectron microscopy and digitonin-permeabilized cells showed that HB-EGF-cyto signals are at the inner nuclear membrane. A short sequence element within the HB-EGF-cyto allows a transmembrane protein to localize to the nuclear envelope. The dominant-active form of Rab5 and Rab11 suppressed nuclear envelope targeting. Collectively, these data demonstrate that membrane-anchored HB-EGF is targeted to the inner nuclear membrane via a retrograde membrane trafficking pathway.

    DOI: 10.1083/jcb.200710022

  • Membrane-anchored growth factor, HB-EGF, on the cell surface targeted to the inner nuclear membrane.

    Hieda, M., Isokane, M., Koizumi, M., Higashi, C., Tachibana, T., Shudou, M., Taguchi, T., Hieda, Y. and Higashiyama, S.

    J.Cell Biol.   180   763 - 769   2008年

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    掲載種別:研究論文(学術雑誌)  

  • Synthesis and degradation of acyl peptide using enzyme from Pseudomonas aeruginosa.

    Islam, NN., Igarashi, K., Tachibana, T., Ooshima, H., and Azuma, M.

    J Biosci Bioeng.   105   282 - 287   2008年

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    掲載種別:研究論文(学術雑誌)  

  • Pitx2 regulates Ad4BP/SF-1 gene transcription in the pituitary gonadotrope through interaction with the intronic enhancer.

    Shima, Y., Zubair, M., Komatsu, T., Oka, S., Yokoyama, C., Tachibana, T., Hjalt, TA., Drouin, J., Morohashi, KI.

    Mol Endocrinol.   22   1633-1646.   2008年

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    掲載種別:研究論文(学術雑誌)  

  • Generation and characterization of a monoclonal antibody against NPI-1 subfamily of importin alpha.

    Tachibana, T., Sakaguchi, N., Miyamoto, Y., Sekimoto,T., Yoneda, Y. and Azuma, M.

    Hybridoma   27   285 - 289   2008年

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    掲載種別:研究論文(学術雑誌)  

  • Generation and characterization of a monoclonal antibody against NPI-1 subfamily of importin alpha. 査読

    Tachibana, T., Sakaguchi, N., Miyamoto, Y., Sekimoto,T., Yoneda, Y. and Azuma, M.

    Hybridoma   27   285 - 289   2008年

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    掲載種別:研究論文(学術雑誌)  

  • Synthesis and degradation of acyl peptide using enzyme from Pseudomonas aeruginosa. 査読

    Islam, NN., Igarashi, K., Tachibana, T., Ooshima, H., and Azuma, M.

    J Biosci Bioeng.   105   282 - 287   2008年

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    掲載種別:研究論文(学術雑誌)  

  • Pitx2 regulates Ad4BP/SF-1 gene transcription in the pituitary gonadotrope through interaction with the intronic enhancer. 査読

    Shima, Y., Zubair, M., Komatsu, T., Oka, S., Yokoyama, C., Tachibana, T., Hjalt, TA., Drouin, J., Morohashi, KI.

    Mol Endocrinol.   22   1633-1646.   2008年

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    掲載種別:研究論文(学術雑誌)  

  • Membrane-anchored growth factor, HB-EGF, on the cell surface targeted to the inner nuclear membrane. 査読

    Hieda, M., Isokane, M., Koizumi, M., Higashi, C., Tachibana, T., Shudou, M., Taguchi, T., Hieda, Y. and Higashiyama, S.

    J.Cell Biol.   180   763 - 769   2008年

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    掲載種別:研究論文(学術雑誌)  

  • Pitx2 regulates Ad4BP/SF-1 gene transcription in the pituitary gonadotrope through interaction with the intronic enhancer. 査読

    Shima, Y, Zubair, M, Komatsu, T, Oka, S, Yokoyama, C, Tachibana, T, Hjalt, TA, Drouin, J, Morohashi, KI

    Mol Endocrinol.   22   1633-1646.   2008年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Generation and characterization of a monoclonal antibody against NPI-1 subfamily of importin alpha. 査読

    Tachibana, T, Sakaguchi, N, Miyamoto, Y, Sekimoto,T, Yoneda, Y, Azuma, M

    Hybridoma   27   285 - 289   2008年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Identification of peripherin as a Akt substrate in neurons 査読

    Konishi Hiroyuki, Namikawa Kazuhiko, Shikata Keiji, Kobatake Yuji, Tachibana Taro, Kiyama Hiroshi

    JOURNAL OF BIOLOGICAL CHEMISTRY   282 ( 32 )   23491 - 23499   2007年08月( ISSN:0021-9258

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1074/jbc.M611703200

  • Identification of peripherin as a Akt substrate in neurons. 査読

    Konishi H, Namikawa K, Shikata K, Kobatake Y, Tachibana T, Kiyama H

    The Journal of biological chemistry   282 ( 32 )   23491 - 23499   2007年08月( ISSN:0021-9258

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1074/jbc.M611703200

    PubMed

  • Short-chain 3-ketoceramides, strong apoptosis inducers against human leukemia HL-60 cells 査読

    Azuma Hideki, Ijichi So, Kataoka Mayuko, Masuda Akira, Izumi Takayuki, Yoshimoto Tetsuya, Tachibana Taro

    BIOORGANIC & MEDICINAL CHEMISTRY   15 ( 8 )   2860 - 2867   2007年04月( ISSN:0968-0896

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.bmc.2007.02.008

  • Short–chain 3-ketoceramides, strong apoptosis inducers against human leukemia HL-60 cells. 査読

    Azuma, H, Ijichi, S, Kataoka, M, Masuda, A, Izumi, T, Yoshimoto, T, Tachibana, T

    Bioorg. Med. Chem.   15 ( 8 )   2860 - 2867   2007年04月( ISSN:0968-0896

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.bmc.2007.02.008

    PubMed

  • Myogenin and the SWI/SNF ATPase Brg1 maintain myogenic gene expression at different stages of skeletal myogenesis 査読

    Ohkawa Yasuyuki, Yoshimura Saori, Higashi Chiduru, Marfella Concetta G. A., Dacwag Caroline S., Tachibana Taro, Imbalzano Anthony N.

    JOURNAL OF BIOLOGICAL CHEMISTRY   282 ( 9 )   6564 - 6570   2007年03月( ISSN:0021-9258

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1074/jbc.M608898200

  • Myogenin and the SWI/SNF ATPase Brg1 maintain myogenic gene expression at different stages of skeletal myogenesis 査読

    Yasuyuki Ohkawa, Saori Yoshimura, Chiduru Higashi, Concetta G. A. Marfella, Caroline S. Dacwag, Taro Tachibana, Anthony N. Imbalzano

    JOURNAL OF BIOLOGICAL CHEMISTRY   282 ( 9 )   6564 - 6570   2007年03月( ISSN:0021-9258

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    掲載種別:研究論文(学術雑誌)  

    Many studies have examined transcriptional regulation during the initiation of skeletal muscle differentiation; however, there is less information regarding transcriptional control during adult myogenesis and during the maintenance of the differentiated state. MyoD and the mammalian SWI/SNF chromatinremodeling enzymes containing the Brg1 ATPase are necessary to induce myogenesis in cell culture models and in developing embryonic tissue, whereas myogenin and Brg1 are critical for the expression of the late genes that induce terminal muscle differentiation. Here, we demonstrate that myogenin also binds to its own promoter during the late stages of embryonic muscle development. As is the case during embryonic myogenesis, MyoD and Brg1 co-localize to the myogenin promoter in primary adult muscle satellite cells. However, in mature myofibers, myogenin and Brg1 are preferentially co-localized to the myogenin promoter. Thus, the myogenin promoter is occupied by different myogenic factors at different times of myogenesis. The relevance of myogenin in the continued expression from its own promoter is demonstrated in culture, where we show that myogenin, in the absence of MyoD, is capable of maintaining its own expression by recruiting the Brg1 ATPase to modify promoter chromatin structure and facilitate myogenin expression. Finally, we utilized in vivo electroporation to demonstrate that Brg1 is required for the continued production of the myogenin protein in newborn skeletal muscle tissue. These findings strongly suggest that the skeletal muscle phenotype is maintained by myogenin and the continuous activity of Brg1-based SWI/SNF chromatin-remodeling enzymes.

    DOI: 10.1074/jbc.M608898200

  • Identification of peripherin as a novel AKT substrate in neurons.

    Konishi, H., Namikawa, K., Shikata, K., Kobatake, Y., Tachibana, T. and Kiyama, H.

    J. Biol. Chem.   282   23491 - 23499   2007年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Short&#8211;chain 3-ketoceramides, strong apoptosis inducers against human leukemia HL-60 cells.

    Azuma, H., Ijichi, S., Kataoka, M., Masuda, A., Izumi, T., Yoshimoto, T. and Tachibana, T.

    Bioorg. Med. Chem.   15   2860 - 2867   2007年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Myogenin and the SWI/SNF atpase BRG1 maintain myogenic gene expression at different stages of skeletal myogenesis.

    Ohkawa, Y., Yoshimura, S., Higashi, C., Marfella, C.G., Dacwag, C.S., Tachibana, T. and Imbalzano, A

    J. Biol. Chem.   282   6564 - 6570   2007年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Identification of peripherin as a novel AKT substrate in neurons. 査読

    Konishi, H., Namikawa, K., Shikata, K., Kobatake, Y., Tachibana, T. and Kiyama, H.

    J. Biol. Chem.   282   23491 - 23499   2007年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Short–chain 3-ketoceramides, strong apoptosis inducers against human leukemia HL-60 cells. 査読

    Azuma, H., Ijichi, S., Kataoka, M., Masuda, A., Izumi, T., Yoshimoto, T. and Tachibana, T.

    Bioorg. Med. Chem.   15   2860 - 2867   2007年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Myogenin and the SWI/SNF atpase BRG1 maintain myogenic gene expression at different stages of skeletal myogenesis. 査読

    Ohkawa, Y., Yoshimura, S., Higashi, C., Marfella, C.G., Dacwag, C.S., Tachibana, T. and Imbalzano, A

    J. Biol. Chem.   282   6564 - 6570   2007年

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    掲載種別:研究論文(学術雑誌)  

  • Cell-cycle-dependent dynamics of nuclear pores: pore-free islands and lamins 査読

    Maeshima Kazuhiro, Yahata Kazuhide, Sasaki Yoko, Nakatomi Reiko, Tachibana Taro, Hashikawa Tsutomu, Imamoto Fumio, Imamoto Naoko

    JOURNAL OF CELL SCIENCE   119 ( 21 )   4442 - 4451   2006年11月( ISSN:0021-9533

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1242/jcs.03207

  • Cell-cycle-dependent dynamics of nuclear pores: pore-free islands and lamins

    Kazuhiro Maeshima, Kazuhide Yahata, Yoko Sasaki, Reiko Nakatomi, Taro Tachibana, Tsutomu Hashikawa, Fumio Imamoto, Naoko Imamoto

    JOURNAL OF CELL SCIENCE   119 ( 21 )   4442 - 4451   2006年11月( ISSN:0021-9533

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    掲載種別:研究論文(学術雑誌)  

    Nuclear pores are sophisticated gateways on the nuclear envelope that control macromolecular transport between the cytoplasm and nucleoplasm. So far the structural and functional aspects of nuclear pores have been extensively studied, but their distribution and density, which might reflect nuclear organization and function, remain unknown. Here, we report the cell-cycle-dependent dynamics of nuclear pores. Large distinct subdomains lacking nuclear pores are present on the nuclear surface of HeLaS3 cells in early cell-cycle stages. Such 'pore-free islands' gradually become dispersed in G1-S phase. Surprisingly, the islands are enriched with inner nuclear membrane proteins lamin A/C and emerin, but exclude lamin B. Lamin-A/C-enriched pore-free islands were also observed in human normal diploid fibroblasts and several cell lines, showing the generality of this phenomenon. Knockdown and ectopic expression analyses demonstrated that lamin A/C, but not emerin, plays an essential structural and regulatory role in the nuclear pore distribution and the formation of pore-free islands. These data thus provide strong evidence that the dynamics of nuclear pores are regulated by the reorganization of inner nuclear structures.

    DOI: 10.1242/jcs.03207

  • Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation 査読

    Uchimura Yasuhiro, Ichimura Takaya, Uwada Junsuke, Tachibana Taro, Sugahara Satoko, Nakao Mitsuyoshi, Saitoh Hisato

    JOURNAL OF BIOLOGICAL CHEMISTRY   281 ( 32 )   23180 - 23190   2006年08月( ISSN:0021-9258

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1074/jbc.M602280200

  • Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation. 査読

    Yasuhiro Uchimura, Takaya Ichimura, Junsuke Uwada, Taro Tachibana, Satoko Sugahara, Mitsuyoshi Nakao, Hisato Saitoh

    The Journal of biological chemistry   281 ( 32 )   23180 - 90   2006年08月( ISSN:0021-9258

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    掲載種別:研究論文(学術雑誌)   国際・国内誌:国際誌  

    Small ubiquitin-related modifiers, SUMO-2/3 and SUMO-1, are involved in gene regulation and nuclear structures. However, little is known about the roles of SUMO, in heterochromatin formation of mammalian cells. Here we demonstrate that SUMOs directly interact with human MCAF1, which forms complexes with either the methyl-CpG-binding protein MBD1 or SETDB1, which trimethylates histone H3 at lysine 9 (H3-K9) in the presence of MCAF1. Modification of MBD1 with either SUMO-2/3 or SUMO-1 facilitated the interaction between MBD1 and MCAF1, suggesting that SUMOylation links the methylation of DNA and histones. In a cultured human cell line, SUMOs were localized in MBD1- and MCAF1-containing heterochromatin regions that were enriched in trimethyl-H3-K9 and the heterochromatin proteins HP1beta and HP1gamma. Specific knockdown of either SUMO-2/3 or SUMO-1 induced dissociation of MCAF1, trimethyl-H3-K9, and the HP1 proteins from the MBD1-containing heterochromatin foci, suggesting a requirement for SUMOs for heterochromatin assembly. These findings provide insights into the roles of SUMOylation in the regulation of heterochromatin formation and gene silencing.

    PubMed

  • Unique anti-apoptotic activity of EAAC1 in injured motor neurons 査読

    Kiryu-Seo Sumiko, Gamo Kazushige, Tachibana Taro, Tanaka Kohichi, Kiyama Hiroshi

    EMBO JOURNAL   25 ( 14 )   3411 - 3421   2006年07月( ISSN:0261-4189

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1038/sj.emboj.7601225

  • The microphthalmia-associated transcription factor requires SWI/SNF enzymes to activate melanocyte-specific genes 査読

    de la Serna Ivana L., Ohkawa Yasuyuki, Higashi Chiduru, Dutta Chaitali, Osias Jules, Kommajosyula Naveen, Tachibana Taro, Imbalzano Anthony N.

    JOURNAL OF BIOLOGICAL CHEMISTRY   281 ( 29 )   20233 - 20241   2006年07月( ISSN:0021-9258

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1074/jbc.M512052200

  • The microphthalmia-associated transcription factor requires SWI/SNF enzymes to activate melanocyte-specific genes

    Ivana L. de la Serna, Yasuyuki Ohkawa, Chiduru Higashi, Chaitali Dutta, Jules Osias, Naveen Kommajosyula, Taro Tachibana, Anthony N. Imbalzano

    JOURNAL OF BIOLOGICAL CHEMISTRY   281 ( 29 )   20233 - 20241   2006年07月( ISSN:0021-9258

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    掲載種別:研究論文(学術雑誌)  

    The microphthalmia transcription factor (Mitf) activates melanocyte-specific gene expression, is critical for survival and proliferation of melanocytes during development, and has been described as an oncogene in malignant melanoma. SWI/SNF complexes are ATP-dependent chromatin-remodeling enzymes that play a role in many developmental processes. To determine the requirement for SWI/SNF enzymes in melanocyte differentiation, we introduced Mitf into fibroblasts that inducibly express dominant negative versions of the SWI/SNF ATPases, Brahma or Brahma-related gene 1 (BRG1). These dominant negative SWI/SNF components have been shown to inhibit gene activation events that normally require SWI/SNF enzymes. We found that Mitf-mediated activation of a subset of endogenous melanocyte-specific genes required SWI/SNF enzymes but that cell-cycle regulation occurred independently of SWI/SNF function. Activation of tyrosinase-related protein 1, a melanocyte-specific gene, correlated with SWI/SNF-dependent changes in chromatin accessibility at the endogenous locus. Both BRG1 and Mitf could be localized to the tyrosinase-related protein 1 and tyrosinase promoters by chromatin immunoprecipitation, whereas immunofluorescence and immunoprecipitation experiments indicate that Mitf and BRG1 co-localized in the nucleus and physically interacted. Together these results suggest that Mitf can recruit SWI/SNF enzymes to melanocyte- specific promoters for the activation of gene expression via induced changes in chromatin structure at endogenous loci.

    DOI: 10.1074/jbc.M512052200

  • MAb 1B6, 2A11, 6A12, 7E11, and 8A12 anti-human Nup 62 査読

    Tachibana T

    HYBRIDOMA   25 ( 3 )   173 - 173   2006年06月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)   共著区分:単著  

  • MAb 1B6, 2A11, 6A12, 7E11, and 8A12 anti-human Nup 62 査読

    Tachibana T

    HYBRIDOMA   25 ( 3 )   173 - 173   2006年06月( ISSN:1554-0014

     詳細を見る

    掲載種別:研究論文(学術雑誌)   共著区分:単著  

  • MAb 1B6, 2A11, 6A12, 7E11, and 8A12 anti-human Nup 62 査読

    T Tachibana

    HYBRIDOMA   25 ( 3 )   173 - 173   2006年06月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

  • In situ SUMOylation analysis reveals a modulatory role of RanBP2 in the nuclear rim and PML bodies. 査読

    Saitoh N, Uchimura Y, Tachibana T, Sugahara S, Saitoh H, Nakao M

    Experimental cell research   312 ( 8 )   1418 - 30   2006年05月( ISSN:0014-4827

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    掲載種別:研究論文(学術雑誌)  

    PubMed

  • In situ SUMOylation analysis reveals a modulatory role of RanBP2 in the nuclear rim and PML bodies 査読

    Saitoh N, Uchimura Y, Tachibana T, Sugahara S, Saitoh H, Nakao M

    EXPERIMENTAL CELL RESEARCH   312 ( 8 )   1418 - 1430   2006年05月( ISSN:0014-4827

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.yexcr.2006.01.013

  • In situ SUMOylation analysis reveals a modulatory role of RanBP2 in the nuclear rim and PML bodies

    N Saitoh, Y Uchimura, T Tachibana, S Sugahara, H Saitoh, M Nakao

    EXPERIMENTAL CELL RESEARCH   312 ( 8 )   1418 - 1430   2006年05月( ISSN:0014-4827

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    掲載種別:研究論文(学術雑誌)  

    SUMO modification plays a critical role in a number of cellular functions including nucleocytoplasmic transport, gene expression, cell cycle and formation of subnuclear structures such as promyelocytic leukemia (PML) bodies. In order to identify the sites where SUMOylation takes place in the cell, we developed an in situ SUMOylation assay using a semi-intact cell system and subsequently combined it with siRNA-based knockdown of nucleoporin RanBP2, also known as Nup358, which is one of the known SUMO E3 proteins. With the in situ SUMOylation assay, we found that both nuclear rim and PML bodies, besides mitotic apparatuses, are major targets for active SUMOylation. The ability to analyze possible SUMO conjugation sites would be a valuable tool to investigate where SUMO E3-like activities and/or SUMO substrates exist in the cell. Specific knockdown of RanBP2 completely abolished SUMOylation along the nuclear rim and dislocated RanGAP1 from the nuclear pore complexes. interestingly, the loss of RanBP2 markedly reduced the number of PML bodies, in contrast to other, normal-appearing nuclear compartments including the nuclear lamina, nucleolus and chromatin, suggesting a novel link between RanBP2 and PML bodies. SUMOylation facilitated by RanBP2 at the nuclear rim may be a key step for the formation of a particular subnuclear organization. Our data imply that SUMO E3 proteins like RanBP2 facilitate spatio-temporal SUMOylation for certain nuclear structure and function. (c) 2006 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.yexcr.2006.01.013

  • Functional analysis of nuclear pore complex protein Nup62/p62 using monoclonal antibodies. 査読

    Fukuhara T, Sakaguchi N, Katahira J, Yoneda Y, Ogino K, Tachibana T

    Hybridoma (2005)   25 ( 2 )   51 - 9   2006年04月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2006.25.51

    PubMed

  • Functional analysis of nuclear pore complex protein Nup62/p62 using monoclonal antibodies 査読

    Fukuhara T, Sakaguchi N, Katahira J, Yoneda Y, Ogino K, Tachibana T

    HYBRIDOMA   25 ( 2 )   51 - 59   2006年04月( ISSN:1554-0014

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Functional analysis of nuclear pore complex protein Nup62/p62 using monoclonal antibodies 査読

    Fukuhara T, Sakaguchi N, Katahira J, Yoneda Y, Ogino K, Tachibana T

    HYBRIDOMA   25 ( 2 )   51 - 59   2006年04月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

  • Functional analysis of nuclear pore complex protein Nup62/p62 using monoclonal antibodies 査読

    T Fukuhara, N Sakaguchi, J Katahira, Y Yoneda, K Ogino, T Tachibana

    HYBRIDOMA   25 ( 2 )   51 - 59   2006年04月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    The nuclear pore complex (NPC) is an enormous structure embedded in the double membrane of the nuclear envelope that acts as a passageway for nucleocytoplasmic transport. The vertebrate NPC is comprised of about 30 unique proteins. Nup62/p62, a major component of the NPC, has been reported to interact directly with several nuclear transport factors, including importin-beta and NTF2. However, it has not been shown how the interaction of Nup62/p62 with transport factors is involved in nucleocytoplasmic transport. The present study reports on the preparation of monoclonal antibodies (MAbs) directed against human Nup62/p62 and a functional analysis of Nup62/p62 using antibodies in living cells. Hybridomas producing the antibodies were produced by the hybridization of mouse myeloma cells with medial iliac lymph node cells from an immunized rat. These MAbs specifically recognized Nup62/p62 as evidenced by immunoblotting analysis using a nuclear membrane fraction. In the immunostaining using MAbs, a punctuate nuclear rim staining pattern was observed. Moreover, cytoplasmic injected-anti-Nup62/p62 MAbs were rapidly targeted to the nuclear pore of cultured cells and some of them inhibited normal cell division, causing the formation of abnormal nuclei. The antibodies described in this study provide the means for immunochemical analyses of the NPC protein Nup62/p62 in mammalian cells, and represent useful molecular tools that should permit a better understanding of the biological roles and cellular dynamics of this protein in nucleocytoplasmic transport, cell division, and nuclear organization.

  • Lipase-catalyzed preparation of optically active 1 '-acetoxychavicol acetates and their structure-activity relationships in apoptotic activity against human leukemia HL-60 cells 査読

    Azuma H, Miyasaka K, Yokotani T, Tachibana T, Kojima-Yuasa A, Matsui-Yuasa I, Ogino K

    BIOORGANIC & MEDICINAL CHEMISTRY   14 ( 6 )   1811 - 1818   2006年03月( ISSN:0968-0896

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.bmc.2005.10.029

  • Identification of peripherin as a novel AKT substrate in neurons.

    Konishi, H., Namikawa, K., Shikata, K., Kobatake, Y., Tachibana, T. and Kiyama, H.

    J. Biol. Chem.   282   23491 - 23499   2006年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation.

    Uchimura, Y., Ichimura, T., Uwada, J., Tachibana, T., Sugahara, S., Nakao, M. and Saitoh, H.

    J. Biol. Chem.   281   23180 - 23190   2006年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Cell cycle-dependent dynamics of nuclear pore: pore-free islands and lamins.

    Maeshima, K., Yahata, K., Sasaki, Y., Nakatomi, R., Tachibana, T., Hashikawa, T., Imamoto, F. and Im

    J. Cell Sci.   119   4442 - 4451   2006年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Unique anti-apoptotic activity of EAAC1 in injured motor neurons.

    Kiryu-Seo, S., Gamo, K., Tachibana, T., Tanaka, K. and Kiyama, H.

    EMBO J.   25   3411 - 3421   2006年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • The microphthalmia-associated transcription factor (MITF) requires SWI/SNF enzymes to activate melanocyte specific genes.

    de la Serna, I. L.,Ohkawa, Y., Higashi, C., Dutta, C., Osias, J., Nommajosyula, N., Tachibana, T. a

    J. Biol. Chem.   281   20233 - 20241   2006年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • In situ SUMOylation analysis reveals a modulatory role of RanBP2 in the nuclear rim and PML bodies.

    Saitoh, N., Uchimura, Y., Tachibana, T., Sugahara, S., Saitoh, H. and Nakao, M.

    Exp. Cell Res.   312   1418 - 1430   2006年

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    掲載種別:研究論文(学術雑誌)  

  • Functional Analysis of the Nuclear Pore Complex Protein Nup62/p62 Using Monoclonal Antibodies

    Fukuhara, T., Sakaguchi, N., Katahira, J., Yoneda, Y., Ogino, K. and Tachibana, T.

    Hybridoma   25   51 - 59   2006年

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    掲載種別:研究論文(学術雑誌)  

  • Cell cycle-dependent dynamics of nuclear pore: pore-free islands and lamins. 査読

    Maeshima, K., Yahata, K., Sasaki, Y., Nakatomi, R., Tachibana, T., Hashikawa, T., Imamoto, F. and Im

    J. Cell Sci.   119   4442 - 4451   2006年

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    掲載種別:研究論文(学術雑誌)  

  • Identification of peripherin as a novel Akt substrate in neurons 査読

    Konishi Hiroyuki, Namikawa Kazuhiko, Shikata Keiji, Kobatake Yuji, Tachibana Taro, Kiyama Hiroshi

    NEUROSCIENCE RESEARCH   55   S177 - S177   2006年( ISSN:0168-0102

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    掲載種別:研究論文(学術雑誌)  

  • Unique anti-apoptotic activity of EAAC1 in injured motor neurons. 査読

    Kiryu-Seo, S., Gamo, K., Tachibana, T., Tanaka, K. and Kiyama, H.

    EMBO J.   25   3411 - 3421   2006年

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    掲載種別:研究論文(学術雑誌)  

  • The microphthalmia-associated transcription factor (MITF) requires SWI/SNF enzymes to activate melanocyte specific genes. 査読

    de la Serna, I. L.,Ohkawa, Y., Higashi, C., Dutta, C., Osias, J., Nommajosyula, N., Tachibana, T. a

    J. Biol. Chem.   281   20233 - 20241   2006年

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    掲載種別:研究論文(学術雑誌)  

  • Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation. 査読

    Uchimura, Y., Ichimura, T., Uwada, J., Tachibana, T., Sugahara, S., Nakao, M. and Saitoh, H.

    J. Biol. Chem.   281   23180 - 23190   2006年

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    掲載種別:研究論文(学術雑誌)  

  • In situ SUMOylation analysis reveals a modulatory role of RanBP2 in the nuclear rim and PML bodies. 査読

    Saitoh, N., Uchimura, Y., Tachibana, T., Sugahara, S., Saitoh, H. and Nakao, M.

    Exp. Cell Res.   312   1418 - 1430   2006年

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    掲載種別:研究論文(学術雑誌)  

  • Identification of peripherin as a novel AKT substrate in neurons. 査読

    Konishi, H., Namikawa, K., Shikata, K., Kobatake, Y., Tachibana, T. and Kiyama, H.

    J. Biol. Chem.   282   23491 - 23499   2006年

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    掲載種別:研究論文(学術雑誌)  

  • Identification of peripherin as a novel Akt substrate in neurons 査読

    Konishi Hiroyuki, Namikawa Kazuhiko, Shikata Keiji, Kobatake Yuji, Tachibana Taro, Kiyama Hiroshi

    NEUROSCIENCE RESEARCH   55   S177 - S177   2006年( ISSN:0168-0102

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    掲載種別:研究論文(学術雑誌)  

  • Functional Analysis of the Nuclear Pore Complex Protein Nup62/p62 Using Monoclonal Antibodies 査読

    Fukuhara, T., Sakaguchi, N., Katahira, J., Yoneda, Y., Ogino, K. and Tachibana, T.

    Hybridoma   25   51 - 59   2006年

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    掲載種別:研究論文(学術雑誌)  

  • Identification of peripherin as a novel Akt substrate in neurons 査読

    Hiroyuki Konishi, Kazuhiko Namikawa, Keiji Shikata, Yuji Kobatake, Taro Tachibana, Hiroshi Kiyama

    NEUROSCIENCE RESEARCH   55   S177 - S177   2006年( ISSN:0168-0102

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    掲載種別:研究論文(学術雑誌)  

  • Unique anti-apoptotic activity of EAAC1 in injured motor neurons. 査読

    Kiryu-Seo, S, Gamo, K, Tachibana, T, Tanaka, K, Kiyama, H

    EMBO J.   25   3411 - 3421   2006年

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    掲載種別:研究論文(学術雑誌)  

  • Specific monoclonal antibody against the nuclear pore complex protein, Nup98 査読

    Fukuhara T, Ozaki T, Shikata K, Katahira J, Yoneda Y, Ogino K, Tachibana T

    HYBRIDOMA   24 ( 5 )   244 - 247   2005年10月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

  • Specific monoclonal antibody against the nuclear pore complex protein, nup98. 査読

    Fukuhara T, Ozaki T, Shikata K, Katahira J, Yoneda Y, Ogino K, Tachibana T

    Hybridoma (2005)   24 ( 5 )   244 - 7   2005年10月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2005.24.244

    PubMed

  • MAb 2H10, anti-Nup98 査読

    Tachibana T

    HYBRIDOMA   24 ( 5 )   272 - 272   2005年10月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)   共著区分:単著  

  • Specific monoclonal antibody against the nuclear pore complex protein, Nup98 査読

    Fukuhara T, Ozaki T, Shikata K, Katahira J, Yoneda Y, Ogino K, Tachibana T

    HYBRIDOMA   24 ( 5 )   244 - 247   2005年10月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

  • MAb 2H10, anti-Nup98 査読

    Tachibana T

    HYBRIDOMA   24 ( 5 )   272 - 272   2005年10月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)   共著区分:単著  

  • MAb 2H10, anti-Nup98 査読

    T Tachibana

    HYBRIDOMA   24 ( 5 )   272 - 272   2005年10月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

  • Specific monoclonal antibody against the nuclear pore complex protein, nup98. 査読

    Fukuhara T, Ozaki T, Shikata K, Katahira J, Yoneda Y, Ogino K, Tachibana T

    Hybridoma (2005)   24 ( 5 )   244 - 247   2005年10月( ISSN:1554-0014

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2005.24.244

    PubMed

  • Specific monoclonal antibody against the nuclear pore complex protein, Nup98 査読

    T Fukuhara, T Ozaki, K Shikata, J Katahira, Y Yoneda, K Ogino, T Tachibana

    HYBRIDOMA   24 ( 5 )   244 - 247   2005年10月( ISSN:1554-0014

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    掲載種別:研究論文(学術雑誌)  

    Nup98 is a component of nuclear pore complexes, which are large protein assemblies embedded in the nuclear envelope. Previous studies have shown that Nup98 interacts with several transport factors and plays a critical part in nuclear trafficking. However, the mechanism by which Nup98 contributes to nuclear trafficking is not clear. The present study reports on the preparation of a monoclonal antibody (MAb) directed against human Nup98. The antibody was produced by the hybridization of mouse myeloma cells with lymph node cells from an immunized rat. This antibody, MAb 21110, specifically recognized Nup98, as evidenced by immunoblotting using a nuclear membrane fraction. In immunostaining using MAb 21110, a punctuate nuclear rim staining pattern was observed. This MAb will be useful in immunoblotting and immunolocalization experiments in various cells and tissues, as well as further analyses of the biological function and cellular dynamics of this protein.

  • Nuclear-cytoplasmic shuttling of a RING-IBR protein RBCK1 and its functional interaction with nuclear body proteins. 査読

    Tatematsu K, Yoshimoto N, Koyanagi T, Tokunaga C, Tachibana T, Yoneda Y, Yoshida M, Okajima T, Tanizawa K, Kuroda S

    The Journal of biological chemistry   280 ( 24 )   22937 - 44   2005年06月( ISSN:0021-9258

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1074/jbc.M413476200

    PubMed

  • Nuclear-cytoplasmic shuttling of a RING-IBR protein RBCK1 and its functional interaction with nuclear body proteins 査読

    Kenji Tatematsu, Nobuo Yoshimoto, Tomoyoshi Koyanagi, Chiharu Tokunaga, Taro Tachibana, Yoshihiro Yoneda, Minoru Yoshida, Toshihide Okajima, Katsuyuki Tanizawai, Shun'ichi Kuroda

    Journal of Biological Chemistry   280 ( 24 )   22937 - 22944   2005年06月( ISSN:0021-9258

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    掲載種別:研究論文(学術雑誌)  

    The intracellular localization of a RING-IBR protein, RBCK1, possessing DNA binding and transcriptional activities, has been investigated. The endogenous RBCK1 was found in both the cytoplasm and nucleus. Particularly in the nucleus, it was localized in the granular structures, most likely nuclear bodies. In contrast, the over-expressed RBCK1 was detected exclusively in the cytoplasm. When the cells were treated with leptomycin B, the over-expressed RBCK1 accumulated in the nuclear bodies. These results suggest that RBCK1 possesses the signal sequences responsible for the nuclear-cytoplasmic translocation. Mutational analysis of RBCK1 has indicated that an N-terminal region containing Leu-142 and Leu-145 and a C-terminal one containing the RING-IBR domain serve as the nuclear export and localization signals, respectively. Thus, RBCK1 is a transcription factor dynamically shuttling between cytoplasm and nucleus. Furthermore, RBCK1 was found to interact with nuclear body proteins, CREB-binding protein (CBP), and promyelocytic leukemia protein (PML). Coexpression of RBCK1 with CBP significantly enhanced the transcriptional activity of RBCK1. Although PML per se showed no effect on the transcriptional activity of RBCK1, the CBP-enhanced activity was repressed by coexpression with PML, presumably through the interaction of PML and CBP. Taken together, our data demonstrate that RBCK1 is involved in transcriptional machinery in the nuclear bodies, and its transcriptional activity is regulated by nucleocytoplasmic shuttling. © 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

    DOI: 10.1074/jbc.M413476200

    PubMed

  • Extracellular signal-dependent nuclear import of STAT3 is mediated by various importin alphas. 査読

    Ushijima R, Sakaguchi N, Kano A, Maruyama A, Miyamoto Y, Sekimoto T, Yoneda Y, Ogino K, Tachibana T

    Biochemical and biophysical research communications   330 ( 3 )   880 - 6   2005年05月( ISSN:0006-291X

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.bbrc.2005.03.063

    PubMed

  • Extracellular signal-dependent nuclear import of STAT3 is mediated by various importin alpha s 査読

    Ushijima R, Sakaguchi N, Kano A, Maruyama A, Miyamoto Y, Sekimoto T, Yoneda Y, Ogino K, Tachibana T

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   330 ( 3 )   880 - 886   2005年05月( ISSN:0006-291X

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    掲載種別:研究論文(学術雑誌)  

  • Extracellular signal-dependent nuclear import of STAT3 is mediated by various importin alpha s 査読

    Ushijima R, Sakaguchi N, Kano A, Maruyama A, Miyamoto Y, Sekimoto T, Yoneda Y, Ogino K, Tachibana T

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   330 ( 3 )   880 - 886   2005年05月( ISSN:0006-291X

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    掲載種別:研究論文(学術雑誌)  

  • Extracellular signal-dependent nuclear import of STAT3 is mediated by various importin alpha s 査読

    R Ushijima, N Sakaguchi, A Kano, A Maruyama, Y Miyamoto, T Sekimoto, Y Yoneda, K Ogino, T Tachibana

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   330 ( 3 )   880 - 886   2005年05月( ISSN:0006-291X

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    掲載種別:研究論文(学術雑誌)  

    The signal transducer and activator of transcription 3 (STAT3) is a transcription factor that is involved in a variety of biological functions. STAT3 is activated by cytokines and growth factors via the phosphorylation of a tyrosine residue, dimerization, and subsequent nuclear translocation. However, the mechanism of its nuclear translocation is unclear. A study of the cytokine-stimulated import of STAT3 into the nucleus is reported herein. An oncostatin M (OSM)-dependent nuclear import assay system was first established in living cells. Using this system, we demonstrated that the microinjection of the importin alpha 5/NPI-1 mutant, an anti-importin P antibody, and the RanQ69L mutant inhibited the nuclear import of STAT3. Second, we showed that tyrosine-phosphorylated STAT3 associates, not only with importin alpha 5/NPI-1 but also with other importin alpha s, as a result of OSM stimulation, as evidenced by a solution binding assay. These findings suggest that the extracellular signal-dependent nuclear transport of STAT3 is mediated by various importin alpha s, importin beta, and Ran. (c) 2005 Elsevier Inc. All rights reserved.

  • The RNA binding protein TLS is translocated to dendritic spines by mGluR5 activation and regulates spine morphology 査読

    Fujii R, Okabe S, Urushido T, Inoue K, Yoshimura A, Tachibana T, Nishikawa T, Hicks GG, Takumi T

    CURRENT BIOLOGY   15 ( 6 )   587 - 593   2005年03月( ISSN:0960-9822

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.cub.2005.01.058

  • Enhanced nuclear import and transfection efficiency of plasmid DNA using streptavidin-fused importin-beta 査読

    Nagasaki T, Kawazu T, Tachibana T, Tamagaki S, Shinkai S

    JOURNAL OF CONTROLLED RELEASE   103 ( 1 )   199 - 207   2005年03月( ISSN:0168-3659

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.jconrel.2004.11.024

  • Enhanced nuclear import and transfection efficiency of plasmid DNA using streptavidin-fused importin-beta

    T Nagasaki, T Kawazu, T Tachibana, S Tamagaki, S Shinkai

    JOURNAL OF CONTROLLED RELEASE   103 ( 1 )   199 - 207   2005年03月( ISSN:0168-3659

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    掲載種別:研究論文(学術雑誌)  

    in order to enhance the nuclear import of exogenous genes, novel plasmid DNA/importin-beta conjugates, which consist of a biotinylated plasmid. DNA and a recombinant streptavidin-fused importin-beta, were prepared. The spacer length between plasmid DNA and biotin and the number of introduced biotin were adjusted. The microinjection of plasmid DNA/importin-beta conjugates into the cytoplasm of NIH3T3 cells resulted in the nuclear localization of conjugates and the higher expression efficiency, compared to intact plasmid DNA alone. These results indicate that plasmid DNA/importin-beta conjugates would be an important tool to enhance the nuclear localization of exogenous DNA in non-viral gene delivery system. (c) 2004 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.jconrel.2004.11.024

  • The RNA binding protein TLS is translocated to dendritic spines by mGluR5 activation and regulates spine morphology 査読

    R Fujii, S Okabe, T Urushido, K Inoue, A Yoshimura, T Tachibana, T Nishikawa, GG Hicks, T Takumi

    CURRENT BIOLOGY   15 ( 6 )   587 - 593   2005年03月( ISSN:0960-9822

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    掲載種別:研究論文(学術雑誌)  

    Neuronal dendrites, together with dendritic spines, exhibit enormously diverse structure [1]. Selective targeting and local translation of mRNAs in dendritic spines have been implicated in synapse remodeling or synaptic plasticity [2, 3]. The mechanism of mRNA transport to the postsynaptic site is a fundamental question in local dendritic translation [4, 5]. TLS (translocated in liposarcoma), previously identified as a component of hnRNP complexes, unexpectedly showed somatodendritic localization in mature hippocampal pyramidal neurons. In the present study, TLS was translocated to dendrites and was recruited to dendrites not only via microtubules but also via actin filaments. In mature hippocampal pyramidal neurons, TLS accumulated in the spines at excitatory postsynapses upon mGIuR5 activation, which was accompanied by an increased RNA content in dendrites. Consistent with the in vitro studies, TLS-null hippocampal pyramidal neurons exhibited abnormal spine morphology and lower spine density. Our results indicate that TLS participates in mRNA sorting to the dendritic spines induced by mGIuR5 activation and regulates spine morphology to stabilize the synaptic structure.

    DOI: 10.1016/j.cub.2005.01.058

  • Lipase-catalyzed Preparation of Optically Active 1'-Acetoxychavicol Acetates and their Structure-Activity Relationships in Apoptotic Activity against Human Leukemia HL-60 Cells.

    Azuma H, Miyasaka K, Yokotani T, Tachibana T, Kojima-Yuasa A, Matsui-Yuasa I, and Ogino K.

    Bioorg. Med. Chem.   14   1811 - 1818   2005年

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    掲載種別:研究論文(学術雑誌)  

  • Extracellular signal-dependent nuclear import of STAT3 is mediated by various importin alphas.

    Ushijima R, et al.

    Biochem Biophys Res Commun.   330   880 - 886   2005年

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    掲載種別:研究論文(学術雑誌)  

  • Nuclear-cytoplasmic shuttling of a RING-IBR protein RBCK1 and its functional interaction with nuclear body proteins.

    Tatematsu K, et al.

    J Biol Chem.   280   22937 - 22944   2005年

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    掲載種別:研究論文(学術雑誌)  

  • The RNA Binding Protein TLS Is Translocated to Dendritic Spines by mGluR5 Activation and Regulates Spine Morphology.

    Fujii R,et al.

    Curr. Biol.   15   587 - 593   2005年

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    掲載種別:研究論文(学術雑誌)  

  • Enhanced nuclear import and transfection efficiency of plasmid DNA using streptavidin-fused importin-beta.

    Nagasaki T, Kawazu T, Tachibana T, Tamagaki S and Shinkai S.

    J. Control Release   103   199 - 207   2005年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Enhanced nuclear import and transfection efficiency of plasmid DNA using streptavidin-fused importin-beta. 査読

    Nagasaki T, Kawazu T, Tachibana T, Tamagaki S and Shinkai S.

    J. Control Release   103   199 - 207   2005年

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    掲載種別:研究論文(学術雑誌)  

  • The RNA Binding Protein TLS Is Translocated to Dendritic Spines by mGluR5 Activation and Regulates Spine Morphology. 査読

    Fujii R,et al.

    Curr. Biol.   15   587 - 593   2005年

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    掲載種別:研究論文(学術雑誌)  

  • Nuclear-cytoplasmic shuttling of a RING-IBR protein RBCK1 and its functional interaction with nuclear body proteins. 査読

    Tatematsu K, et al.

    J Biol Chem.   280   22937 - 22944   2005年

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    掲載種別:研究論文(学術雑誌)  

  • Lipase-catalyzed Preparation of Optically Active 1'-Acetoxychavicol Acetates and their Structure-Activity Relationships in Apoptotic Activity against Human Leukemia HL-60 Cells. 査読

    Azuma H, Miyasaka K, Yokotani T, Tachibana T, Kojima-Yuasa A, Matsui-Yuasa I, and Ogino K.

    Bioorg. Med. Chem.   14   1811 - 1818   2005年

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    掲載種別:研究論文(学術雑誌)  

  • Extracellular signal-dependent nuclear import of STAT3 is mediated by various importin alphas. 査読

    Ushijima R, et al.

    Biochem Biophys Res Commun.   330   880 - 886   2005年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Lipase-catalyzed Preparation of Optically Active 1'-Acetoxychavicol Acetates and their Structure-Activity Relationships in Apoptotic Activity against Human Leukemia HL-60 Cells. 査読

    Azuma H, Miyasaka K, Yokotani T, Tachibana T, Kojima-Yuasa A, Matsui-Yuasa I, Ogino K

    Bioorg. Med. Chem.   14   1811 - 1818   2005年

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    掲載種別:研究論文(学術雑誌)  

  • Specific monoclonal antibody against nuclear import factor, importin alpha1/Rch1. 査読

    Kamikubo Y, Sakaguchi N, Shikata K, Furuta M, Miyamoto Y, Imamoto N, Yoneda Y, Ogino K, Tachibana T

    Hybridoma and hybridomics   23 ( 5 )   301 - 4   2004年10月( ISSN:1536-8599

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2004.23.301

    PubMed

  • Specific monoclonal antibody against nuclear import factor, importin alpha1/Rch1. 査読

    Kamikubo Y, Sakaguchi N, Shikata K, Furuta M, Miyamoto Y, Imamoto N, Yoneda Y, Ogino K, Tachibana T

    Hybridoma and hybridomics   23 ( 5 )   301 - 4   2004年10月( ISSN:1536-8599

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/hyb.2004.23.301

    PubMed

  • Fatal hypothermia related vacuolation of hormone-producing cells in the anterior pituitary. 査読

    Ishikawa T, Miyaishi S, Tachibana T, Ishizu H, Zhu BL, Maeda H

    Legal medicine (Tokyo, Japan)   6 ( 3 )   157 - 63   2004年07月( ISSN:1344-6223

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.legalmed.2004.05.004

    PubMed

  • (3Z)-2-Acetylamino-3-octadecen-1-ol as a potent apoptotic agent against HL-60 cells. 査読

    Niiro H, Azuma H, Tanago S, Matsumura K, Shikata K, Tachibana T, Ogino K

    Bioorganic & medicinal chemistry   12 ( 1 )   45 - 51   2004年01月( ISSN:0968-0896

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    掲載種別:研究論文(学術雑誌)  

    PubMed

  • (3Z)-2-Acetylamino-3-octadecen-1-ol as a potent apoptotic agent against HL-60 cells. 査読

    Niiro H, Azuma H, Tanago S, Matsumura K, Shikata K, Tachibana T, Ogino K

    Bioorganic & medicinal chemistry   12 ( 1 )   45 - 51   2004年01月( ISSN:0968-0896

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.bmc.2003.10.040

    PubMed

  • (3Z)-2-Acetylamino-3-octadecan-1-ol as a potent apoptotic agent againstHL-60 cells.

    Niiro,H., Azuma,H., Tanago,T. Matsumura,K., Shikata,K., Tachibana,T. and Ogino,K.

    Bioorg.Med.Chem.   12   45 - 51   2004年

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    掲載種別:研究論文(学術雑誌)  

  • Specific monoclonal antibody against nuclear import factor, importin alpha1/Rch1.

    Kamikubo,Y., et al.

    Hybridoma and Hybridomics   23   301 - 304   2004年

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    掲載種別:研究論文(学術雑誌)  

  • Generation of a rat monoclonal antibody specific for importin alpha3/Qip1. 査読

    Sakaguchi N, Miyamoto Y, Yoneda Y, Ogino K, Tachibana T

    Hybridoma and hybridomics   22 ( 6 )   397 - 400   2003年12月( ISSN:1536-8599

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/153685903771797110

    PubMed

  • Generation of a rat monoclonal antibody specific for importin alpha3/Qip1. 査読

    Sakaguchi N, Miyamoto Y, Yoneda Y, Ogino K, Tachibana T

    Hybridoma and hybridomics   22 ( 6 )   397 - 400   2003年12月( ISSN:1536-8599

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1089/153685903771797110

    PubMed

  • Synthesis and potent antileukemic activities of N-lactylsphingosine and N-lactyldihydrosphingosine. 査読

    Azuma H, Takao R, Shikata K, Niiro H, Tachibana T, Ogino K

    Journal of medicinal chemistry   46 ( 16 )   3445 - 7   2003年07月( ISSN:0022-2623

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/jm030125p

    PubMed

  • Synthesis and potent antileukemic activities of N-lactylsphingosine and N-lactyldihydrosphingosine. 査読

    Azuma H, Takao R, Shikata K, Niiro H, Tachibana T, Ogino K

    Journal of medicinal chemistry   46 ( 16 )   3445 - 7   2003年07月( ISSN:0022-2623

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/jm030125p

    PubMed

  • Apoptotic activities of C2-ceramide and C2-dihydroceramide homologues against HL-60 cells. 査読

    Shikata K, Niiro H, Azuma H, Ogino K, Tachibana T

    Bioorganic & medicinal chemistry   11 ( 13 )   2723 - 8   2003年07月( ISSN:0968-0896

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    PubMed

  • Apoptotic activities of C2-ceramide and C2-dihydroceramide homologues against HL-60 cells. 査読

    Shikata K, Niiro H, Azuma H, Ogino K, Tachibana T

    Bioorganic & medicinal chemistry   11 ( 13 )   2723 - 8   2003年07月( ISSN:0968-0896

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/s0968-0896(03)00228-1

    PubMed

  • Total syntheses of symbioramide derivatives from l-serine and their antileukemic activities. 査読

    Azuma H, Takao R, Niiro H, Shikata K, Tamagaki S, Tachibana T, Ogino K

    The Journal of organic chemistry   68 ( 7 )   2790 - 7   2003年04月( ISSN:0022-3263

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/jo0206824

    PubMed

  • Total syntheses of symbioramide derivatives from l-serine and their antileukemic activities. 査読

    Azuma H, Takao R, Niiro H, Shikata K, Tamagaki S, Tachibana T, Ogino K

    The Journal of organic chemistry   68 ( 7 )   2790 - 7   2003年04月( ISSN:0022-3263

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/jo0206824

    PubMed

  • Can nuclear localization signals enhance nuclear localization of plasmid DNA? 査読

    Nagasaki T, Myohoji T, Tachibana T, Futaki S, Tamagaki S

    Bioconjugate chemistry   14 ( 2 )   282 - 6   2003年03月( ISSN:1043-1802

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/bc025602h

    PubMed

  • Can nuclear localization signals enhance nuclear localization of plasmid DNA? 査読

    Nagasaki T, Myohoji T, Tachibana T, Futaki S, Tamagaki S

    Bioconjugate chemistry   14 ( 2 )   282 - 6   2003年03月( ISSN:1043-1802

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/bc025602h

    PubMed

  • Synthesis of non-natural C2-homo-ceramide and its apoptotic activity against HL-60 cells. 査読

    Shikata K, Niiro H, Azuma H, Tachibana T, Ogino K

    Bioorganic & medicinal chemistry letters   13 ( 4 )   613 - 6   2003年02月( ISSN:0960-894X

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    PubMed

  • Synthesis of non-natural C2-homo-ceramide and its apoptotic activity against HL-60 cells. 査読

    Shikata K, Niiro H, Azuma H, Tachibana T, Ogino K

    Bioorganic & medicinal chemistry letters   13 ( 4 )   613 - 6   2003年02月( ISSN:0960-894X

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/s0960-894x(02)01026-0

    PubMed

  • Synthesis and potent antileukemic activities of N-Lactyl-sphingosine and N-Lactyl-dihydrosphingosine.

    Azuma,H., Takao,R., Shikata,K., Niiro,H., Tachibana,T. and Ogino,K.

    J.Med.Chem.   46   3445 - 3447   2003年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Generation of a rat monoclonal antibody specific for importin alpha3/Qip1.

    Sakaguchi,N., Miyamoto,Y., Yoneda,Y., Ogino,K. and Tachibana,T.

    Hybridoma and Hybridomics   22 ( 6 )   2003年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Can nuclear localization signals enhance nuclear localization of plasmid DNA?

    Nagasaki,T. Myohoji,T. Tachibana,T. Futaki,S. and Tamagaki,S.

    Bioconjug.Chem.   14   282 - 286   2003年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Apoptotic activities of C2-ceramide and C2-dihydroceramide homologues against HL-60 cells.

    Shikata,K., Niiro,H., Azuma,H., Ogino,K. and Tachibana,T.

    Bioorg.Med.Chem.   11   2723 - 2728   2003年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Total syntheses of Symbioramide derivatives from L-serine and their antileukemic activities.

    Azuma,H.,Takao,R., Niiro,H., Shikata,K., Tamagaki,S., Tachibana,T. and Ogino,K.

    J.Org.Chem.   68   2790 - 2797   2003年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Synthesis of non-natural C2-homo-ceramide and its apoptotic activity against HL-60 cells.

    Shikata,K., Niiro,H., Azuma,H., Tachibana,T. and Ogino,K.

    Bioorg.Med.Chem.Lett.   13   613 - 616   2003年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • A new technique to co-localise membrane proteins with Homer/vesl. 査読

    Hiroaki Y, Nishikawa K, Mitsuoka K, Tachibana T, Sobue K, Doi T, Fujiyoshi Y

    Biochemical and biophysical research communications   295 ( 3 )   756 - 65   2002年07月( ISSN:0006-291X

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

    PubMed

  • A new technique to co-localise membrane proteins with Homer/vesl.

    Hiroaki,Y., Nishikawa,K., Mitsuoka,K., Tachibana,T., Sobue,K.,et al.

    Biochem.Biophys.Res.Commun.   295   756 - 765   2002年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Synthesis of novel and non-natural ceramide analogues derived from L-glutamic acid.

    -

    Tetrahedron   58   5803 - 5809   2002年

     詳細を見る

    掲載種別:研究論文(学術雑誌)   共著区分:単著  

  • Nuclear import of the U1A splicesome protein is mediated by importin a/b and ran in living mammalian cells.

    Hieda,M., Tachibana,T., Fukumoto,M., and Yoneda,Y

    J.Biol.Chem.   276   16824 - 16832   2001年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • The small GTP-binding protein TC10 promotes nerve elongation in neuronal cells, and its expression is induced during nerve regeneration in rats.

    Tanabe,K., Tachibana,T., Yamashita,T., Yong Ho Che, Yoneda,Y. et al.

    J.Neurosci.   20   4138 - 4144   2000年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Characterization of the nuclear transport of a novel leucine-rich acidic nuclear protein-like protein.

    Matsubae,M., Kurihara,T., Tachibana,T., Imamoto,N. and Yoneda,Y.

    FEBS Lett.   468   171 - 175   2000年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • *Recycling of importin-alpha from the nucleus is suppressed by loss of RCC1 function in living mammalian cells. (*CSF Award)

    Tachibana,T., Hieda,M., Miyamoto,Y., Kose,S., Imamoto,N. et al.

    Cell Struc. Func.   25   115 - 123   2000年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Necleocytoplasmic shuttling of the aryl hydrocarbon receptor.

    Ikuta,T., Tachibana,T., Watanabe,J., Yoshida,M.,Yoneda,Y. et al.

    J.Biochem.   127   503 - 509   2000年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Involvement of unique leucine-zipper motif of PSD-Zip45(Homer 1c/vesl-1L) in group 1 metabotropic glutamate receptor clustering.

    Tadokoro,S., Tachibana,T., Imanaka,T., Nishida,W. andSobue,K.

    Proc.Natl.Acad.Sci.USA   96   13801 - 13806   1999年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • b-Catenin can be transported into the nucleus in a Ran-unassisted manner.

    Yokoya,F., Imamoto,N., Tachibana,T. and Yoneda,Y.

    Mol.Biol.Cell   10   1119 - 1131   1999年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • A monoclonal antibody to the C-terminal acidic portion of Ran inhibits both recycling of Ran and nuclear protein import in living cells.

    Tachibana,T.*, Hieda,M.*, Yokoya,F., Kose,S., Imamoto,N. et al.

    J.Cell Biol.   144   645 - 655   1999年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Up-regulation of nuclear protein import by nuclear localization signal sequences in living cells.

    Tachibana,T., Hieda,M. and Yoneda,Y.

    FEBS Lett.   442   235 - 240   1999年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • b-Subunit of nuclear pore-targeting complex (Importin-b can be exported from the nucleus in a Ran-unassisted manner.

    Kose,S., Imamoto,N., Tachibana,T., Yoshida,M. and Yoneda,Y.

    J.Biol.Chem.   274   3946 - 3952   1999年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Molecular Shape and ATP Binding Activity of Rat p50, a Putative Mammalian Homologue of RuvB DNA Helicase.

    Kikuchi,N., Gohshi,T., Kawahire,S., Tachibana,T., Yoneda,Y. et al.

    J.Biochem.   125   487 - 497   1999年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Nuclear transport factor p10/NTF2 functions as a Ran-GDP dissociation inhibitor (Ran-GDI).

    Yamada,M., Tachibana,T., Imamoto,N. and Yoneda,Y.

    Curr.Biol.   8   1339 - 1342   1998年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Nuclear localization and export signals of the human aryl hydrocarbon receptor.

    Ikuta,T., Eguchi,H., Tachibana,T., Yoneda,Y. and Kawajiri,K.

    J.Biol.Chem.   273   2895 - 2904   1998年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Differentialmodes of nuclear localization signal (NLS) recognition by three distinct classes of NLS receptors.

    Miyamoto,Y., Imamoto,N., Sekimoto,T., Tachibana,T., Seki,T. et al.

    J.Biol.Chem.   272   26375 - 26381   1997年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Ran-unassisted nuclear migration of a 97-kD component of nuclear pore-targeting complex.

    Kose,S., Imamoto,N., Tachibana,T., Shimamoto,T. and Yoneda,Y.

    J.Cell Biol.   139   841 - 849   1997年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • A nuclear localization signal of human aryl hydrocarbon receptor nuclear translocator/hypoxia-inducible factor 1b is a novel bipartite type recognized by the two components of nuclear pore-targeting complex.

    Eguchi,H., Ikuta,T., Tachibana,T., Yoneda,Y. and Kawajiri,K.

    J.Biol.Chem.   272   17640 - 17647   1997年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Essential role of active nuclear transport in apoptosis.

    Yasuhara,N., Eguchi,Y., Tachibana,T., Imamoto,N., Yoneda,Y. et al.

    Genes to Cells   2   55 - 64   1997年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Exogenously injected nuclear import factor p10/NTF2 inhibits signal-mediated nuclear import and export of proteins in living cells.

    Tachibana,T., Hieda,M., Sekimoto,T. and Yoneda,Y.

    FEBS Lett.   397   177 - 182   1996年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Subcellular distribution and phosphorylation of the nuclear localization signal binding protein.

    Kawahire,S., Tachibana,T., Umemoto,M., Yoneda,Y., Imai,N. et al.

    Exp.Cell Res.   222   385 - 394   1996年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Interferon-g-dependent nuclear import of Stat1 is mediated by the GTPase activity of Ran/TC4.

    Sekimoto,T., Nakajima,K., Tachibana,T., Hirano,T. and Yoneda,Y.

    J.Biol.Chem.   271   31017 - 31020   1996年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Ranと核蛋白質輸送

    立花太郎、米田悦啓

    実験医学   14 ( 2 )   260 - 263   1996年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Induction of neurite outgrowth by MAP kinase in PC12 cells.

    Fukuda,M., Gotoh,Y., Tachibana,T., Dell,K., Hattori,S. et al.

    Oncogene   11   239 - 244   1995年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • In vivo evidence for involvement of a 58kDa component of nuclear pore-targeting complex in nuclear protein import.

    Imamoto,N., Shimamoto,T., Takao,T., Tachibana,T., Matsubae,M.et al.

    EMBO J.   14   3617 - 3626   1995年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Nuclear pore-targeting complex binds to nuclear pore after association with a karyophile.

    Imamoto,N., Shimamoto,T., Kose,S., Takao,T., Tachibana,T.et al.

    FEBS Lett.   368   415 - 419   1995年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • A karyophilic protein forms a stable complex with cytoplasmic components prior to nuclear pore binding.

    Imamoto,N., Tachibana,T., Matsubae,M. and Yoneda,Y.

    J.Biol.Chem.   270   8559 - 8565   1995年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Interconversion of bacteriochlorophyll c aggregates in solid films upon organic vapor treatment.

    Uehara,K., Tachibana,T., Tsunooka,M. and Ozaki,Y.

    Photochem. Photobiol.   62   496 - 501   1995年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Loss of RCC1 leads to the suppression of nuclear protein import in living cells.

    Tachibana,T., Imamoto,N., Seino,H., Nishimoto,T. and Yoneda,Y.

    J.Biol.Chem.   269   24542 - 24545   1994年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

  • Structure and interconversion of bacteriochlorophyll c aggregates in solid films.

    Uehara,K., Tachibana,T., Ozaki,Y. and Tsunooka,M.

    Reseach in Photosynthesis   1   85 - 88   1992年

     詳細を見る

    掲載種別:研究論文(学術雑誌)  

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MISC(その他記事)

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講演・口頭発表等

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産業財産権等

  • 免疫賦活能を有する酵母、及び、食品又は飼料

    伊藤千夏、鈴木啓三、浜田健一、東雅之、立花太郎、古川周平、中島亮一、渡邉肇、小林紗由美

     詳細を見る

    産業財産権の種類:特許権 

    出願番号:特願2009-13864 

    公開番号:特開2010-284085 

    公表番号:特許第5608344号 

産学官連携可能研究(シーズ)概要

  • 高効率モノクローナル抗体の作製

    2001-

     詳細を見る

    共同研究の希望:民間等他機関

    交流の種別:技術相談, 受託研究, 共同研究  

    活用分野・応用方法等:非常に短期間で高品質のモノクローナル抗体を作製する。

    簡便・高効率なモノクローナル抗体の作製技術提供

科研費

  • 生活習慣病モデルメダカを用いた歯周病合併症の予防抑制効果を示す食品由来物質の探索

    基盤研究(B)  2023年

  • 特異的抗体を用いた転移性がん細胞表面分子の同定と創薬応用

    基盤研究(C)  2022年04月

  • 生活習慣病モデルメダカを用いた歯周病合併症の予防抑制効果を示す食品由来物質の探索

    基盤研究(B)  2022年

  • 二重特異性抗体を用いたBNCTホウ素デリバリーシステムの開発

    基盤研究(B)  2019年

  • 世界的流行を示す腸管毒素原性大腸菌O169の新規接着因子による人獣共通感染の検証

    基盤研究(B)  2019年

  • 1細胞ラビット組換え抗体作製技術を用いた新規がん幹細胞表面マーカー分子の探索

    基盤研究(C)  2019年

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受託研究

  • 次世代ワクチン創薬科学に資する免疫測定基盤技術の創成

    AMED (国立研究開発法人 日本医療研究開発機構)  新興・再興感染症研究基盤創生事業(多分野融合研究領域)  2023年

  • レポーターHBVを駆使したB型肝炎ウイルス増殖機構の解析と創薬ターゲットの探索・同定に資する研究

    AMED(国立大学法人 神戸大学)  保健衛生医療調査等推進事業費補助金/肝炎等克服実用化研究事業(B型肝炎創薬実用化等研究事業)  2023年

  • 希少疾患かつ指定難病の嚢胞性リンパ管腫に対する画期的治療薬の開発研究

    AMED (国立大学法人 富山大学)  難治性疾患実用化研究事業  2023年

その他補助金等

  • HBV感染を阻害するモノクローナル抗体の作製

    国立研究開発法人日本医療研究開発機構(AMED)  肝炎等克服実用化研究事業(B型肝炎創薬実用化等研究事業)  2023月

  • HBV感染を阻害するモノクローナル抗体の作製

    国立研究開発法人日本医療研究開発機構(AMED)  肝炎等克服実用化研究事業(B型肝炎創薬実用化等研究事業)  2022月

  • HBVの感染を阻害するモノクローナル抗体の開発

    国立研究開発法人日本医療研究開発機構(AMED)  肝炎等克服実用化研究事業(B型肝炎創薬実用化等研究事業)  2021月

  • HBVの感染を阻害するモノクローナル抗体の開発

    国立研究開発法人日本医療研究開発機構(AMED)  肝炎等克服実用化研究事業(B型肝炎創薬実用化等研究事業)  2020月

  • HBVの感染を阻害するモノクローナル抗体の開発

    国立研究開発法人日本医療研究開発機構(AMED)  肝炎等克服実用化研究事業(B型肝炎創薬実用化等研究事業)  2019月

  • HBVの感染を阻害するモノクローナル抗体の開発

    国立研究開発法人日本医療研究開発機構(AMED)  肝炎等克服実用化研究事業(B型肝炎創薬実用化等研究事業)  2018月

  • HBVの感染を阻害するモノクローナル抗体の開発

    国立研究開発法人日本医療研究開発機構(AMED)  肝炎等克服実用化研究事業(B型肝炎創薬実用化等研究事業)  2017月

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研究員受入実績

  • 2022年度  研究員数:1名

  • 2021年度  研究員数:1名

  • 2020年度  研究員数:1名

  • 2019年度  研究員数:1名

担当授業科目

  • バイオテクノロジー概論

    2023年度   週間授業   大学

  • バイオデザイン

    2023年度   集中講義   大学院

  • 物質化学生命系特別研究

    2023年度   集中講義   大学院

  • 特別演習

    2023年度   集中講義   大学院

  • 物質化学生命系特別研究第2

    2023年度   集中講義   大学院

  • 物質化学生命系特別演習第2

    2023年度   集中講義   大学院

  • 卒業研究Ⅱ

    2023年度   集中講義   大学

  • バイオ工学実験Ⅱ

    2023年度   週間授業   大学

  • バイオ英語演習

    2023年度   週間授業   大学

  • 化学バイオ工学特論Ⅱ

    2023年度   集中講義   大学

  • 創薬生命工学特論

    2022年度   週間授業   大学院

  • 物質化学生命系特別演習第1

    2022年度   集中講義   大学院

  • 卒業研究Ⅰ

    2022年度   集中講義   大学

  • 化学バイオ工学特論Ⅰ

    2022年度   集中講義   大学

  • 化学バイオ工学概論

    2022年度   集中講義   大学

  • 物質化学生命系特別演習

    2022年度   集中講義   大学院

  • 物質化学生命系特別研究 (化学バイオ工学分野)

    2022年度   集中講義   大学院

  • 物質化学生命系特別演習第2 (化学バイオ工学分野)

    2022年度   集中講義   大学院

  • 卒業研究

    2022年度   集中講義   大学

  • バイオテクノロジー概論

    2022年度   週間授業   大学

  • バイオ工学実験

    2022年度   週間授業   大学

  • バイオ英語演習

    2022年度   週間授業   大学

  • 化学バイオ工学特論

    2022年度   集中講義   大学

  • 特別演習(化学生物系2回生)

    2022年度   集中講義   大学院

  • 前期特別研究(化学生物系)

    2022年度   集中講義   大学院

  • 後期特別研究(化学生物系)

    2022年度   集中講義   大学院

  • ゼミナール(化学生物系)

    2022年度   集中講義   大学院

  • 化学バイオ工学特論

    2018年度     大学

  • 創薬分子工学特論

    2018年度     大学院

  • バイオテクノロジー概論

    2018年度     大学

  • 細胞生物学

    2018年度     大学

  • バイオ工学実験

    2018年度     大学

  • 化学バイオ工学概論

    2018年度     大学

▼全件表示

学外での担当授業科目

  • 保健学研究共通特講Ⅰ・V

    2023年04月
    機関名:神戸大学

     詳細を見る

    科目区分:大学院専門科目  国名:日本国

  • 保健学研究共通特講Ⅰ・V

    2022年04月
    機関名:神戸大学

     詳細を見る

    科目区分:大学院専門科目 

  • 保健学研究共通特講Ⅰ・V

    2021年04月
    機関名:神戸大学

     詳細を見る

    科目区分:大学院専門科目 

  • 保健学研究共通特講Ⅰ・V

    2020年04月
    機関名:神戸大学

     詳細を見る

    科目区分:大学院専門科目 

  • 超分子化学

    機関名:大阪市立大学工学部

  • 技術と生命

    機関名:大阪市立大学工学部

  • 蛋白質科学

    機関名:大阪市立大学工学部

  • 医薬品科学

    機関名:大阪市立大学工学部

  • 生物科学入門

    機関名:大阪市立大学工学部

  • 細胞情報学特論

    機関名:大阪市立大学大学院工学研究科

▼全件表示

FD活動

  • FD活動への貢献  2017年度

     詳細を見る

    学内各FD集会参加

所属院生等の論文発表集計

  • 2023年度

    所属大学院生発表数:2件

  • 2022年度

    学部・学域生発表数:1件  所属大学院生発表数:2件

論文・研究指導集計

  • 2023年度

    卒業論文指導数:4名  卒業論文審査数:4件

    博士前期課程学生指導数:3名 

    修士論文審査数(主査):3件  修士論文審査数(副査):10件

  • 2022年度

    卒業論文指導数:3名  卒業論文審査数:3件

    博士前期課程学生指導数:6名  博士後期課程学生指導数:0名

    修士論文審査数(主査):3件  修士論文審査数(副査):4件

    博士論文審査数(主査):0件  博士論文審査数(副査):0件

社会貢献活動

  • 抗体医薬 -21世紀の魔法の弾丸-

    役割:講師

    大阪市立大学文化交流センター  公開講座「温故知新」  大阪市立大学文化交流センター  2017年11月

     詳細を見る

    対象: 社会人・一般

    種別:講演会

    参加者数:80(人)

    バイオテクノロジーの進歩により、これまでとは全く異なるタイプの医薬品が登場しました。それが抗体医薬です。関節リウマチやガンなどで高い治療効果をあげている抗体医薬について、基礎からわかりやすく解説します。

外国人受入実績

  • 2019年度

    留学生受入数 :1名

  • 2018年度

    留学生受入数 :1名

  • 2017年度

    留学生受入数 :1名

役職

  • 全学管理職

    大阪公立大学

    学長補佐  2024年04月 - 継続中

  • 全学管理職

    大阪市立大学

    学長特別補佐  2021年04月 - 2022年03月

  • 部局内役職

    大阪市立大学大学院工学研究科化学生物系専攻

    専攻長  2021年04月 - 2022年03月

その他

  • 職務経歴

    2017年10月 - 継続中

      詳細を見る

    大阪市立大学大学院工学研究科 教授

  • 職務経歴

    2007年04月 - 2017年09月

      詳細を見る

    大阪市立大学大学院工学研究科 准教授

  • 職務経歴

    2001年04月 - 2007年03月

      詳細を見る

    大阪市立大学大学院工学研究科 助教授