Updated on 2024/04/14

写真a

 
HOSOKAWA Chie
 
Organization
Graduate School of Science Department of Chemistry Professor
School of Science Department of Chemistry
Title
Professor
Affiliation
Institute of Science
Affiliation campus
Sugimoto Campus

Position

  • Graduate School of Science Department of Chemistry 

    Professor  2022.04 - Now

  • School of Science Department of Chemistry 

    Professor  2022.04 - Now

Degree

  • Doctor(Engineering) ( Osaka University )

Research Areas

  • Nanotechnology/Materials / Nanobioscience

  • Nanotechnology/Materials / Fundamental physical chemistry

  • Informatics / Life, health and medical informatics

  • Nanotechnology/Materials / Optical engineering and photon science

Research Interests

  • 顕微分光

  • Fluorescence Imaging

  • Neurophotonics

  • 神経情報コード

  • Brain / Neuron

  • Biophysics

  • 生体生命情報学

  • Nanobiology

  • Optical Tweezers

Professional Memberships

  • THE JAPAN SOCIETY OF APPLIED PHYSICS

      Domestic

  • THE BIOPHYSICAL SOCIETY OF JAPAN

      Domestic

  • THE CHEMICAL SOCIETY OF JAPAN

      Domestic

  • 日本分光学会

      Domestic

  • Society for Neuroscience

      Overseas

  • 応用物理学会有機分子・バイオエレクトロニクス分科会

      Domestic

  • THE JAPAN NEUROSCIENCE SOCIETY

      Domestic

  • THE INSTITUTE OF ELECTRICAL ENGINEERS OF JAPAN

  • THE SOCIETY OF INSTRUMENT AND CONTROL ENGINEERS

  • Society of Nano Science and Technology

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Committee Memberships (off-campus)

  • 分野別専門委員   日本生物物理学会  

    2024.01 - 2024.12 

  • Organizing Committee   21st IUPAB Congress 2024 (IUPAB2024)  

    2023.06 - Now 

  • 量子ビームによる機能性ミクロ構造創生とバイオメディカルシステム応用技術調査専門委員会   一般社団法人電気学会  

    2023.05 - 2025.04 

  • Organizing Committee   The 11th International Conference on Molecular Electronics & Bioelectronics (M&BE11)  

    2023.04 - Now 

  • 2023・2024年度 代議員   日本生物物理学会  

    2023.04 - 2025.03 

  • Symposium Chair   16th International Symposium on Nanomedicine (ISNM2023)  

    2023.01 - 2023.11 

  • 有機分子・バイオエレクトロニクス分科会 庶務幹事   公益社団法人応用物理学会  

    2022.04 - 2024.03 

  • 専門調査員   文部科学省 科学技術・学術政策研究所  

    2022.04 - Now 

  • R041バイオ・分子・ナノテクノロジー融合委員会委員   日本学術振興会 産学協力委員会  

    2022.04 - Now 

  • 第61期、第62期 代議員   公益社団法人応用物理学会  

    2022.04 - 2024.03 

  • 「分子ナノテクノロジー」第174委員会委員   日本学術振興会 産学協力研究委員会  

    2021.08 - 2022.03 

  • 光・量子デバイス技術委員会 「量⼦ビームによるナノ構造・界⾯形成とバイオメディカル応⽤」技術調査専門委員会委員   電気学会  

    2021.05 - 2023.04 

  • 分野別専門委員   日本生物物理学会  

    2021.01 - 2022.12 

  • 理事   日本生物物理学会  

    2019.06 - 2021.05 

  • 光・量子デバイス技術委員会 「量子ビームによるナノ構造形成とその医療・バイオ応用」技術調査専門委員会委員   電気学会  

    2019.05 - 2021.04 

  • 代議員   日本生物物理学会  

    2019.04 - 2021.03 

  • 幹事   日本分光学会 関西支部  

    2018.04 - Now 

  • 「生物物理」会誌編集委員   日本生物物理学会  

    2017.04 - 2018.12 

  • 常任幹事   応用物理学会 有機分子・バイオエレクトロニクス分科会  

    2015.04 - 2017.03 

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Awards

  • 第9回 2013年 第51回年会 若手招待講演者

    細川 千絵

    2013.09   日本生物物理学会  

  • Shiseido Female Researcher Science Grant

    HOSOKAWA Chie

    2013.06  

  • 研究奨励賞

    細川 千絵

    2011.09   計測自動制御学会 生体・生理工学部会  

  • 第20回(2006年春季) 講演奨励賞

    細川 千絵

    2006.09   応用物理学会  

Job Career (off-campus)

  • Osaka Metropolitan University   Department of Chemistry, Graduate School of Science

    2022.04 - Now

  • Osaka City University   Graduate School of Science

    2019.01 - 2022.03

  • National Institute of Advanced Industrial Science and Technology (AIST)   Senior Researcher

    2013.03 - 2018.12

  • JST   PRESTO researcher

    2006.10 - 2011.03

  • National Institute of Advanced Industrial Science and Technology (AIST)   Researcher

    2006.04 - 2013.02

  • Osaka University   Grad. Sch. Eng.   PD

    2006.01 - 2006.03

  • JSPS   JSPS Research Fellow (DC2)

    2003.04 - 2005.03

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Education

  • Osaka University   Grad. Sch. Eng.   Dept. Appl. Phys.   The second semester of doctoral program   Graduated/Completed

    2002.04 - 2005.12

  • Osaka University   Grad. Sch. Eng.   Dept. Appl. Phys.   The first semester of doctoral program   Graduated/Completed

    2000.04 - 2002.03

  • Osaka University   Div. Appl. Sci.   Bachelor's Course   Graduated/Completed

    1996.04 - 2000.03

Papers

  • Mechanisms of neuronal stimulation with a focused nanosecond optical vortex

    Yumi Segawa, Yasushi Tanimoto, Ken-ichi Yuyama, Wataru Minoshima, Kyoko Masui, Takashige Omatsu, Chie Hosokawa

    Optical Manipulation and Structured Materials Conference   12606   126061H   2023.09

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    Publishing type:Research paper (international conference proceedings)   International / domestic magazine:International journal  

    DOI: 10.1117/12.3008385

  • Network-wide neuronal activity under optical trapping of synaptic vesicles in cultured neurons

    Wataru Minoshima, Taketo Yasuda, Kyoko Masui, Chie Hosokawa

    Optical Manipulation and Structured Materials Conference   12606   1260613   2023.09

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    Publishing type:Research paper (international conference proceedings)   International / domestic magazine:International journal  

    DOI: 10.1117/12.3008362

  • Drastic photostability improvement of silica particles impregnated with multiple emitting CdSe quantum dots prepared through efficient surface silanization Reviewed International coauthorship

    N. Murase, C. Hosokawa, C. Li, M. Onishi, T. Sawai

    Colloids and Surfaces A: Physicochemical and Engineering Aspects   654   130084   2022.12( ISSN:09277757

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Numerous studies have been performed on silica coated quantum dots (QDs) for the retention of photostability of QDs over long period of time. However, the photostability of silica coated QDs has been found to be inferior to that of polymer coated QDs under some circumstances, and the quantitative data is missing in prior arts. Here, we report the drastic improvement of photostability of CdSe QDs encapsulated into silica particles by optimizing the preparation conditions. We have prepared silica particles (ca. 60 nm in size) encapsulated with multiple, over dozens of emitting CdSe QDs. The key step is a surface silanization of QDs, where the original ligands are replaced by a partially hydrolyzed alkoxide in hydrophobic solution. The evaluation using single particle detection methods shows that the photostability increases up to 8 times as compared with the polymer coated counterparts by optimizing the molar ratio of alkoxide against QDs upon the silanization. When the ratio is optimum, the packing density of QDs in the silica particle becomes the maximum accompanied by the most obvious red shift of ensemble photoluminescence peak. This indicates a developed silica network in close proximity to the QD surface on the particle is formed. The knowledge acquired from this work is universal, and can be extended to other emitting QDs such as InP and perovskites.

    DOI: 10.1016/j.colsurfa.2022.130084

  • Optical trapping dynamics of nanoparticles by resonant optical response Reviewed

    Ko Ueno, Tatsunori Kishimoto, Kyoko Masui, Chie Hosokawa

    Optical Manipulation and Structured Materials Conference (OMC 2022)   12479   78   2022.12( ISSN:0277786X ( ISBN:9781510660656

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    DOI: 10.1117/12.2659047

  • Recent advances in optical manipulation of cells and molecules for biological science Reviewed

    T. Kishimoto, K. Masui, W. Minoshima, C. Hosokawa

    Journal of Photochemistry and Photobiology C: Photochemistry Reviews   53   100554   2022.12( ISSN:13895567

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Noninvasive and nondestructive techniques for monitoring and manipulating cells or biomolecules are essential for understanding biological processes. Optical methodologies have been used for the noninvasive and nondestructive monitoring of intracellular molecules and manipulation of cellular activities to elucidate the localization and interactions of these biomolecules. Since the pioneering work of Ashkin, optical trapping has been used to study cellular elasticity and mechanical characteristics of intracellular molecules. In recent years, there has been a substantial amount of research on the optical manipulation of nanometer-sized objects, including the manipulation of the assembly of nanomaterials and the enhancement of optical forces with optical resonance effects. In the study of biomolecular manipulation by optical forces, the functions and roles of biomolecules have been clarified by analyzing the changes in cellular functions induced by manipulation. In this review, we focus on recent studies on optical trapping for the manipulation of living cells or biomolecules and introduce techniques for the manipulation of cellular functions using optical forces.

    DOI: 10.1016/j.jphotochemrev.2022.100554

  • Detection of Glutamate Encapsulated in Liposomes by Optical Trapping Raman Spectroscopy Reviewed

    K. Masui, Y. Nawa, S. Tokumitsu, T. Nagano, M. Kawarai, H. Tanaka, T. Hamamoto, W. Minoshima, T. Tani, S. Fujita, H. Ishitobi, C. Hosokawa, Y. Inouye

    ACS Omega   7 ( 11 )   9701 - 9709   2022.03( ISSN:2470-1343

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    The transmission of neuronal information is propagated through synapses by neurotransmitters released from presynapses to postsynapses. Neurotransmitters released from the presynaptic vesicles activate receptors on the postsynaptic membrane. Glutamate acts as a major excitatory neurotransmitter for synaptic vesicles in the central nervous system. Determining the concentration of glutamate in single synaptic vesicles is essential for understanding the mechanisms of neuronal activation by glutamate in normal brain functions as well as in neurological diseases. However, it is difficult to detect and quantitatively measure the concentration of glutamate in single synaptic vesicles owing to their small size, i.e., similar to 40 nm. In this study, to quantitatively evaluate the concentrations of the contents in small membrane-bound vesicles, we developed an optical trapping Raman spectroscopic system that analyzes the Raman spectra of small objects captured using optical trapping. Using artificial liposomes encapsulating glutamate that mimic synaptic vesicles, we investigated whether spontaneous Raman scattered light of glutamate can be detected from vesicles trapped at the focus using optical forces. A 575 nm laser beam was used to simultaneously perform the optical trapping of liposomes and the detection of the spontaneous Raman scattered light. The intensity of Raman scattered light that corresponds to lipid bilayers increased with time. This observation suggested that the number of liposomes increased at the focal point. The number of glutamate molecules in the trapped liposomes was estimated from the calibration curve of the Raman spectra of glutamate solutions with known concentration. This method can be used to measure the number of glutamate molecules encapsulated in synaptic vesicles in situ.

    DOI: 10.1021/acsomega.1c07206

    PubMed

  • Optical trapping of nanoparticles suspended in water with a bull’s eye-type plasmonic chip Reviewed

    細川 千絵

    Proceedings of SPIE   11926   2021

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  • Fluid convection driven by suspended particles in optical trapping Reviewed

    Tetsuro Tsuji, Chie Hosokawa, Tatsunori Kishimoto, Takumi Okubo, Suguru N. Kudoh, Satoyuki Kawano

    Proceedings of SPIE - The International Society for Optical Engineering   11926   1 - 2   2021( ISSN:0277786X ( ISBN:9781510647206

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    We numerically investigate the convection of surrounding fluid in optical trapping of micro- and nanoparticles. The effects of the laser irradiation on the fluid simulation are twofold. First, we take into account the temperature increase of the fluid due the photothermal effect of the solvent, that is, the fluid flow is described by the Navier-Stokes equations under the Boussinesq approximation. Second, we assume that the suspended particles drag the fluid when they are transported by the optical force. This dragging effect is considered in the fluid simulation by adding to the Navier-Stokes equation an external forcing term, which is modelled by considering the counterbalance between the optical scattering force and the Stokes drag. It is shown that the latter effect is dominant under the usual experimental setup in optical trapping of particles with the diameter larger than 0.5 μm. Furthermore, the particle size dependence on the convective flow speed is investigated. The numerical results are supported by optical trapping experiment qualitatively.

    DOI: 10.1117/12.2616108

  • Single Particle Tracking Analysis of Optical Trapping Dynamics of AMPA-type Glutamate Receptors Reviewed

    岸本龍典, 岸本龍典, 工藤卓, 田口隆久, 細川千絵, 細川千絵

    電気学会論文誌 C   141 ( 5 )   668 - 675   2021( ISSN:03854221

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    Publishing type:Research paper (scientific journal)  

    AMPA-type glutamate receptor (AMPAR) is one of neurotransmitter receptors at excitatory synapses in neurons. For realizing the artificial control of synaptic transmission, we have applied optical trapping of quantum-dot (QD) conjugated AMPARs on neurons. Here, we evaluate optical trapping dynamics of QD-AMPARs on neurons by single particle tracking (SPT) analysis. The diffusion coefficient derived from mean square displacement (MSD) at laser focus of QD-AMPARs on neurons decreased with the laser irradiation and the culturing days, which suggests that the molecular dynamics of QD-AMPARs are constrained at the focal spot due to optical trapping forces.

    DOI: 10.1541/ieejeiss.141.668

    CiNii Article

    J-GLOBAL

  • Optical trapping of nanoparticles suspended in water with a bull's eye-type plasmonic chip Reviewed

    Chie Hosokawa, Takashi Koizumi, Tomoya Nagasue, Keiko Tawa

    Proceedings of SPIE - The International Society for Optical Engineering   11926   2021( ISSN:0277786X ( ISBN:9781510647206

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    Publishing type:Research paper (scientific journal)  

    We demonstrate surface plasmon resonance (SPR) based optical trapping of quantum-dot (QD) nanoparticles suspended in water with a bull's eye-type plasmonic chip. The particle dynamics of QD suspensions at the laser focus was evaluated by fluorescence correlation spectroscopy. The average transit time of QD suspensions on the plasmonic chip increased than that on the coverslip, suggesting that single QD was more constrained at the focal spot due to optical trapping enhanced with SPR.

    DOI: 10.1117/12.2616159

  • Deuterated glutamate-mediated neuronal activity on micro-electrode arrays Reviewed

    Wataru Minoshima, Kyoko Masui, Tomomi Tani, Yasunori Nawa, Satoshi Fujita, Hidekazu Ishitobi, Chie Hosokawa, Yasushi Inouye

    Micromachines   11 ( 9 )   830 - 830   2020.09( ISSN:2072-666X

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    Publishing type:Research paper (scientific journal)  

    © 2020 by the authors. The excitatory synaptic transmission is mediated by glutamate (GLU) in neuronal networks of the mammalian brain. In addition to the synaptic GLU, extra-synaptic GLU is known to modulate the neuronal activity. In neuronal networks, GLU uptake is an important role of neurons and glial cells for lowering the concentration of extracellular GLU and to avoid the excitotoxicity. Monitoring the spatial distribution of intracellular GLU is important to study the uptake of GLU, but the approach has been hampered by the absence of appropriate GLU analogs that report the localization of GLU. Deuterium-labeled glutamate (GLU-D) is a promising tracer for monitoring the intracellular concentration of glutamate, but physiological properties of GLU-D have not been studied. Here we study the effects of extracellular GLU-D for the neuronal activity by using primary cultured rat hippocampal neurons that form neuronal networks on microelectrode array. The frequency of firing in the spontaneous activity of neurons increased with the increasing concentration of extracellular GLU-D. The frequency of synchronized burst activity in neurons increased similarly as we observed in the spontaneous activity. These changes of the neuronal activity with extracellular GLU-D were suppressed by antagonists of glutamate receptors. These results suggest that GLU-D can be used as an analog of GLU with equivalent effects for facilitating the neuronal activity. We anticipate GLU-D developing as a promising analog of GLU for studying the dynamics of glutamate during neuronal activity.

    DOI: 10.3390/MI11090830

    PubMed

  • Two-Photon-Excited Emission of Quantum Dots with a Plasmonic Chip Reviewed

    Yuki Omura, Yuki Matsubayashi, Tatsunori Kishimoto, Suguru N. Kudoh, Chie Hosokawa, Keiko Tawa

    Journal of Physical Chemistry C   124 ( 29 )   16076 - 16082   2020.07( ISSN:1932-7447

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    Copyright © 2020 American Chemical Society. Plasmonic chips have wavelength-size periodic structures coated with thin metal films and can provide enhanced electric fields near the chip surfaces. Herein, a plasmonic chip was developed to carry out one-photon-excited emission (1PE) and two-photon-excited emission (2PE). Most studies on plasmon-assisted 2PE are based on the localized surface plasmon resonance. Among the propagated surface plasmon resonances (SPRs), we focused on the grating-coupled SPR. Quantum dots (QD655) dispersed on the silver plasmonic chip of the bull's eye patterns with a 600 nm pitch were excited by a near-infrared (NIR) laser with λ = 1064 nm. The emission intensity plotted against the irradiation light intensity was well fitted to a quadratic function for the plasmonic chip and coverslip. Therefore, the emission excited by NIR light was evaluated as 2PE. The coefficient of the quadratic part for the plasmonic chip was larger than that for the coverslip, and our plasmonic chip was found to promote the two-photon excitation. After correcting the molecule number at illumination spots, the enhancement factor of the 2PE evaluated in the plasmonic chip was found to be greater than 6-fold compared with that in the coverslip. The enhanced 2PE was assisted by the propagated plasmon field in the bull's eye-patterned plasmonic chip.

    DOI: 10.1021/acs.jpcc.0c00826

  • Neuronal electrical activity induced by optical trapping of neurotransmitter receptors on neuron Reviewed

    Tatsunori Kishimoto, Suguru N. Kudoh, Takahisa Taguchi, Chie Hosokawa

    Proceedings of SPIE - The International Society for Optical Engineering   11522   1152217   2020.06

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    Publishing type:Research paper (international conference proceedings)   Kind of work:Joint Work  

    DOI: https://doi.org/10.1117/12.2573759

  • Convection Dynamics Forced by Optical Trapping with a Focused Laser Beam Reviewed

    Chie Hosokawa, Tetsuro Tsuji, Tatsunori Kishimoto, Takumi Okubo, Suguru N. Kudoh, Satoyuki Kawano

    Journal of Physical Chemistry C   124 ( 15 )   8323 - 8333   2020.04( ISSN:1932-7447

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    © 2020 American Chemical Society. Optical trapping dynamics of colloidal particles in solution is essential for understanding laser-induced assembling of molecules and nanomaterials, which contributes to nanofabrication, bioengineering, and microfluidics. In this paper, the importance of the surrounding fluid motion in optical trapping is investigated; that is, we reveal convection fluid dynamics forced by optical trapping with a focused laser beam. The fluid flow in optical trapping is evaluated by both experiments using the particle-image-velocimetry of fluorescent particles in solutions and theoretical consideration based on numerical analysis. A theoretical model consists of Navier-Stokes equations with the Boussinesq approximation that considers the temperature elevation induced by a photothermal effect. Furthermore, the effect of the particle motion induced by the optical force on fluid flow is also included in the analysis by developing a simple one-way homogeneous-type multiphase flow model. From both experimental and theoretical results, it turns out that the fluid flow in optical trapping is caused not only by thermal convection due to the temperature elevation but also by the collective particle motion induced by optical forces. Therefore, the optical forces can induce the large-scale fluid convection, which supports accumulating the target particles to the focal spot.

    DOI: 10.1021/acs.jpcc.9b11663

  • Real-time fluorescence measurement of spontaneous activity in a high-density hippocampal network cultivated on a plasmonic dish Reviewed

    Wataru Minoshima, Chie Hosokawa, Suguru N. Kudoh, Keiko Tawa

    Journal of Chemical Physics   152 ( 1 )   2020.01( ISSN:0021-9606

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    © 2020 Author(s). High-density cultured neuronal networks have been used to evaluate synchronized features of neuronal populations. Voltage-sensitive dye (VSD) imaging of a dissociated cultured neuronal network is a critical method for studying synchronized neuronal activity in single cells. However, the signals of VSD are generally too faint - that is, the signal-to-noise ratio (S/N) is too low - to detect neuronal activity. In our previous research, a silver (Ag) plasmonic chip enhanced the fluorescence intensity of VSD to detect spontaneous neural spikes on VSD imaging. However, no high-density network was cultivated on the Ag plasmonic chip, perhaps because of the chemical instability of the Ag surface. In this study, to overcome the instability of the chip, we used a chemically stable gold (Au) plasmonic dish, which was a plastic dish with a plasmonic chip pasted to the bottom, to observe neuronal activity in a high-density neuronal network. We expected that the S/N in real-time VSD imaging of the Au plasmonic chip would be improved compared to that of a conventional glass-bottomed dish, and we also expected to detect frequent neural spikes. The increase in the number of spikes when inhibitory neurotransmitter receptors were inhibited suggests that the spikes corresponded to neural activity. Therefore, real-time VSD imaging of an Au plasmonic dish was effective for measuring spontaneous network activity in a high-density neuronal network at the spatial resolution of a single cell.

    DOI: 10.1063/1.5131497

    PubMed

  • Neuronal electrical activity induced by optical trapping of neurotransmitter receptors on neuron Reviewed

    Tatsunori Kishimoto, Suguru N. Kudoh, Takahisa Taguchi, Chie Hosokawa

    OPTICAL MANIPULATION AND STRUCTURED MATERIALS CONFERENCE 2020   11522   2020( ISSN:0277-786X ( ISBN:9781510638518

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    Publishing type:Research paper (international conference proceedings)  

    AMPA- type glutamate receptor (AMPAR) is one of neurotransmitter receptors at excitatory synapses in neuronal cell. For realizing the artificial control of synaptic transmission, we have applied optical trapping of quantum-dot (QD) conjugated AMPARs on neuronal cells. Here, we demonstrate simultaneous measurement combined with optical trapping and patch-clamp recordings to evaluate the neuronal electrical activity. The relationship between optical trapping dynamics of QD-AMPARs located on neuronal cells and the neuronal electrical activity was discussed.

    DOI: 10.1117/12.2573759

  • Long-term real-time imaging of a voltage sensitive dye in cultured hippocampal neurons using the silver plasmonic dish Reviewed

    Wataru Minoshima, Chie Hosokawa, Suguru N. Kudoh, Keiko Tawa

    Journal of Photochemistry and Photobiology A: Chemistry   384   111949   2019.11( ISSN:1010-6030

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    © 2019 Elsevier B.V. We have demonstrated the real-time voltage sensitive dye (VSD) imaging of a neuronal network using Di-4-ANEPPS at a 1 ms-time resolution on a silver (Ag) plasmonic dish to improve the signals to noise (S/N) ratio. The plasmonic dish is composed of a culture dish and a metal-coated substrate with a wavelength-order periodical structure on its surface that can enhance excitation field due to the surface plasmon resonance (SPR)-field. In our group, spontaneous neural spikes that are undetectable on a conventional culture dish were frequently detected on a gold (Au) plasmonic dish during real-time VSD imaging. In the present study, Ag and Au plasmonic chips were prepared and tested. Larger fluorescence enhancement of the VSD was obtained on the Ag plasmonic dish compared with on the Au. Number of neural spikes were frequently detected on the Ag plasmonic dish compared to the Au plasmonic dish in the control neurons and after application picrotoxin (PTX), because of the photobleaching rate was suppressed and the noise level decreased by the reduction of illumination light on the Ag surface. Therefore, the use of an Ag plasmonic dish was considered to be suitable for the long-term and real-time observation by VSD imaging.

    DOI: 10.1016/j.jphotochem.2019.111949

  • Learning process for identifying different types of communication via repetitive stimulation: feasibility study in a cultured neuronal network Reviewed

    Yoshi Nishitani, Chie Hosokawa, Yuko Mizuno-Matsumoto, Tomomitsu Miyoshi, Shinichi Tamura

    AIMS Neuroscience   6 ( 4 )   240 - 249   2019.10

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work   International / domestic magazine:International journal  

    DOI: 10.3934/Neuroscience.2019.4.240

  • Asynchronous Multiplex Communication Channels in 2-D Neural Network with Fluctuating Characteristics Reviewed

    Shinichi Tamura, Yoshi Nishitani, Chie Hosokawa, Yuko Mizuno-Matsumoto

    IEEE Transactions on Neural Networks and Learning Systems   30 ( 8 )   2336 - 2345   2019.08( ISSN:2162-237X

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    © 2012 IEEE. Neurons behave like transistors, but have fluctuating characteristics. In this paper, we show that several asynchronous multiplex communication channels can be established in a 2-D mesh neural network with randomly generated weights between eight neighbors. Neurons were simulated by integrate-and-fire neuron models without leakage and with fluctuating refractory period and output delay. If one of the transmitting neuron groups is stimulated, the signal is propagated in the form of spike waves. The corresponding receiving neuron group is able to identify the signal after having learned to form an asynchronous multiplex communication channel. The channel is composed of many intermediate/interstitial neurons working as relays. Each neuron can work as an I/O and as a relay element, i.e., as a multiuse unit. Grouping and synchronic firing is often seen in natural neuronal networks and seems to be effective for stable/robust communication in conjunction with spatial multiplex communication. This communication pattern corresponds to our wet lab experiments on cultured neuronal networks and is similar to sound identification by the ear and mobile adaptive communication systems.

    DOI: 10.1109/TNNLS.2018.2880565

    PubMed

  • Non-uniform stochastic dynamics of nanoparticle clusters at a solid–liquid interface induced by laser trapping Reviewed

    Itsuo Hanasaki, Chie Hosokawa

    Japanese Journal of Applied Physics   58 ( SD )   SDDK07-1 - 8   2019.06( ISSN:0021-4922

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    © 2019 The Japan Society of Applied Physics. We reveal the characteristics of Brownian motion in nanoparticle clusters formed by the optical force fields at a solid-liquid interface based on the trajectory analysis from the microscopy movie data. The characteristics of stochastic motion depend not only on the laser power but also on the location in the clusters. The particles at the focal points exhibit smaller displacements, compared to the surrounding particles that are nevertheless trapped. The nominal diffusion coefficient is larger than the bulk at sufficiently small time scale for sufficiently high laser powers, although it decreases as a function of time because of the confinement. Decomposition of radial and circumferential components of displacements reveals the anisotropy as well as non-uniformity of the dynamics.

    DOI: 10.7567/1347-4065/ab0887

    Other URL: https://doi.org/10.7567/1347-4065/ab0887

  • Anisotropic dynamics of nanoparticles in clusters at a solid-liquid interface by laser trapping Reviewed

    Itsuo Hanasaki, Chie Hosokawa

    Journal of Physics: Conference Series   1220 ( 1 )   012015   2019.05( ISSN:1742-6588

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    Publishing type:Research paper (international conference proceedings)  

    © Published under licence by IOP Publishing Ltd. It is well-recognized that the Brownian motion of particles in fluids is random. Nevertheless, there can be characteristics depending on the specific physical conditions. We analyze the system of nanoparticle clusters formed by the laser trapping force field at the solid-liquid interface, based on the microscopy movie data. Since the laser trapping force field is basically a function of radial distance from the focal point in the two dimension at the liquid-solid interface, we examine the difference of displacement distributions in the radial and circumferential directions. The results show that the basic characteristics in this system depends on the laser power, and there is an anisotropy in the stochastic motion of the nanoparticles.

    DOI: 10.1088/1742-6596/1220/1/012015

  • Time-scale dependent Brownian motion of nanoparticles in clusters at a solid-liquid interface by laser trapping Reviewed

    Itsuo Hanasaki, Chie Hosokawa

    Journal of Physics: Conference Series   1220 ( 1 )   012014   2019.05( ISSN:1742-6588

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    Publishing type:Research paper (international conference proceedings)  

    © Published under licence by IOP Publishing Ltd. Nanoparticles in a cluster trapped by laser-induced force field show Brownian motion at solid-liquid interfaces. The cluster formation means that the particles are highly concentrated. In general, the diffusion coefficients of particles in fluids decrease with substantially high concentration and also in the vicinity of solid walls due to the hydrodynamic effect. The particle trajectory data obtained from the experimental measurements show that the longer time span of observation leads to smaller diffusion coefficient due to the confinement effect. However, they also exhibit higher diffusion coefficient compared to the the bulk condition when evaluated at a sufficiently short time span of the frame interval under the condition of sufficiently high laser powers.

    DOI: 10.1088/1742-6596/1220/1/012054

  • Learning process for identifying different types of communication via repetitive stimulation: feasibility study in a cultured neuronal network Reviewed

    Nishitani Yoshi, Hosokawa Chie, Mizuno-Matsumoto Yuko, Miyoshi Tomomitsu, Tamura Shinichi

    AIMS NEUROSCIENCE   6 ( 4 )   240 - 249   2019( ISSN:2373-8006

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    DOI: 10.3934/Neuroscience.2019.4.240

  • Resonance laser effect on optical trapping of cell surface molecules Reviewed

    Tatsunori Kishimoto, Suguru N. Kudoh, Takahisa Taguchi, Chie Hosokawa

    Proceedings of SPIE - The International Society for Optical Engineering   11141   1114101   2019( ISSN:0277-786X

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    Publishing type:Research paper (international conference proceedings)   Kind of work:Joint Work  

    © Optics & Photonics International Congress 2019. For the purpose of artificial control in synaptic transmission on neuronal cells with optical trapping, we demonstrate optical trapping with resonance effect of nanoparticles and cell surface molecules by simultaneous irradiation with nonresonance and resonance laser beams. The average transit times of quantum-dot (QD) suspensions increased by the simultaneous irradiation with NIR laser and resonance laser, suggesting that single QD was more constrained at the focal spot due to optical trapping potential enhanced with resonance laser irradiation. Moreover, we applied the resonance laser effect to manipulate QD-conjugated AMPA-type glutamate receptors on neuronal cells.

    Other URL: http://orcid.org/0000-0002-0289-4680

  • Preparation and biomedical applications of bright robust silica nanocapsules with multiple incorporated InP/ZnS quantum dots Reviewed

    Li Chunliang, Hosokawa Chie, Suzuki Mariko, Taguchi Takahisa, Murase Norio

    NEW JOURNAL OF CHEMISTRY   42 ( 23 )   18951 - 18960   2018.12( ISSN:1144-0546

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    DOI: 10.1039/c8nj02465k

  • Quantitative Evaluation of Optical Forces by Single Particle Tracking in Slit-like Microfluidic Channels Reviewed

    Fumika Nito, Tetsuya Shiozaki, Ryo Nagura, Tetsuro Tsuji, Kentaro Doi, Chie Hosokawa, Satoyuki Kawano

    Journal of Physical Chemistry C   122 ( 31 )   17963 - 17975   2018.08( ISSN:1932-7447

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    © 2018 American Chemical Society. Optical trapping and manipulation techniques have attracted significant attention in various research fields. Optical forces divided into two terms, such as a scattering force and gradient one, work to push forward and attract objects, respectively. This is a typical property of optical forces. In particular, a tool known as optical tweezers can be created when a laser beam is converged at a focal point, causing strong forces to be generated so as to trap and manipulate small objects. In this study, we propose a novel method to build up assembled structures of polystyrene particles by using optical trapping techniques. Recording trajectories of single particles, the optical forces are quantitatively evaluated using particle tracking velocimetry. Herein, we treat various particle sizes whose diameters range from 1 to 4 μm and expose them to a converged laser beam of 1064 nm wavelength. As a result, both experimental and theoretical results are in good agreement. The behavior of particles is understood in the framework of Ashkin's ray optics. This finding clarifies optical force fields of microparticles distributed in a slit-like microfluidic channel and will be applicable for effectively forming ordered structures in liquids.

    DOI: 10.1021/acs.jpcc.8b02701

  • Effect of correlating adjacent neurons for identifying communications: Feasibility experiment in a cultured neuronal network Reviewed

    Yoshi Nishitani, Chie Hosokawa, Yuko Mizuno-Matsumoto, Tomomitsu Miyoshi, Shinichi Tamura

    AIMS Neuroscience   5 ( 1 )   18 - 31   2018.01( ISSN:2373-8006

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    © 2018 the Author(s). Neuronal networks have fluctuating characteristics, unlike the stable characteristics seen in computers. The underlying mechanisms that drive reliable communication among neuronal networks and their ability to perform intelligible tasks remain unknown. Recently, in an attempt to resolve this issue, we showed that stimulated neurons communicate via spikes that propagate temporally, in the form of spike trains. We named this phenomenon "spike wave propagation". In these previous studies, using neural networks cultured from rat hippocampal neurons, we found that multiple neurons, e.g., 3 neurons, correlate to identify various spike wave propagations in a cultured neuronal network. Specifically, the number of classifiable neurons in the neuronal network increased through correlation of spike trains between current and adjacent neurons. Although we previously obtained similar findings through stimulation, here we report these observations on a physiological level. Considering that individual spike wave propagation corresponds to individual communication, a correlation between some adjacent neurons to improve the quality of communication classification in a neuronal network, similar to a diversity antenna, which is used to improve the quality of communication in artificial data communication systems, is suggested.

    DOI: 10.3934/Neuroscience.2018.1.18

    Other URL: http://orcid.org/0000-0002-0289-4680

  • Optical trapping of quantum-dot conjugated AMPA-type receptors depened on initial assembling states Reviewed

    Tatsunori Kishimoto, Yasuyo Maezawa, Suguru N. Kudoh, Takahisa Taguchi, Chie Hosokawa

    Proceedings of SPIE - The International Society for Optical Engineering   10712   1071204   2018

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    DOI: 10.1117/12.2319362

    Other URL: http://orcid.org/0000-0002-0289-4680

  • Fluorescent imaging of live hippocampal neurons with platinum nanoclusters Reviewed

    Minami Fujieda, Kyoko Masui, Chie Hosokawa, Hidekazu Ishitobi, Yasushi Inouye

    Optics InfoBase Conference Papers   Part F125-JSAP 2018   2018

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    © 2018 OSA - The Optical Society. All rights reserved. Fluorescent imaging is a powerful technique to reveal how target cells or biomolecules work in biological tissues and cells. So far, organic dyes, fluorescent proteins, and quantum dots have been developed and widely used as a fluorescent probe. However, these fluorescent probes do not work well in certain conditions: they have cytotoxicity and steric hindrance due to inclusion of harmful atoms and/or their sizes. Hence, metal nanoclusters, that are composed of several metal atoms and emit fluorescence owing to quantum size effect, have been anticipated as a newly categorized fluorescent probe. Since the fluorescent wavelength depends on their sizes, it is possible to control the fluorescent wavelength by adjusting the number of composed atoms [1].

  • Creation of nanoparticle arrays by integration of nanofluidics and optical forces Reviewed

    Satoshi Nishioka, Tatsunori Kishimoto, Chie Hosokawa, Toshiyuki Kawabata, Takehiro Tsujikawa, Toshiyuki Nomura, Suguru N. Kudoh, Yan Xu

    22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018   1   416 - 418   2018( ISBN:9781510897571

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    Copyright© (2018) by Chemical and Biological Microsystems Society.All rights reserved. Optical forces are well known as providing a noncontact method of manipulating micro- or nano-sized particles. In previous studies, however, it has been reported that the trapping power of the optical tweezers is smaller than that of the movement of the particles (eg. Brownian motion) and it becomes unstable trapping. Since the trapping force decreases in proportion to the size of the target, it is extremely difficult to precisely manipulate nano-sized particles. On the other hand, the use of ultrasmall confined spaces of well-defined nanofluidic environments will provide new mechanisms and technologies for manipulating nano-sized particles in liquid phase. These features contribute to the suppression of the Brownian motion of nanoparticles in 3D nano space. The integration of optical forces and nanofluidics would allow single nanoparticles to be captured, transported and arrayed in nanofluidic systems.

  • Preparation and biomedical applications of bright robust silica nanocapsules with multiple incorporated InP/ZnS quantum dots Reviewed

    Chunliang Li, Chie Hosokawa, Mariko Suzuki, Takahisa Taguchi, Norio Murase

    New Journal of Chemistry   42 ( 23 )   18951 - 18960   2018( ISSN:1144-0546

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    Publishing type:Research paper (scientific journal)  

    © The Royal Society of Chemistry and the Centre National de la Recherche Scientifique. InP-based quantum dots (QDs) have been proposed as an alternative to CdSe-based QDs for both bioimaging and device displays. In this work, we incorporate hydrophilic InP/ZnS QDs into silica nanocapsules. The morphology, number of incorporated QDs, and photoluminescence (PL) properties of the capsules depend on the alkoxide hydrolysis conditions. By selecting a method of QD surface silanization, multiple QDs (typically 3) are incorporated into the nanocapsules (∼40 nm in size) without causing significant deterioration of the initial PL efficiency (up to 36%). Degradation analysis in different buffer solutions indicates that silica nanocapsules have higher stabilities than pristine QDs. The surface of the nanocapsules was modified with carboxyl groups without any change in morphology. Bright PL images with high resolution were obtained after introducing the nanocapsules into rat hippocampal neurons, when compared with commercial Q-trackers (polymer coated CdSe/ZnS QDs).

    DOI: 10.1039/c8nj02465k

  • Enhanced fluorescence microscopy with the Bull's eye-plasmonic chip Reviewed

    Tawa Keiko, Izumi Shota, Sasakawa Chisato, Hosokawa Chie, Toma Mana

    OPTICS EXPRESS   25 ( 9 )   10622 - 10631   2017.05( ISSN:1094-4087

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    DOI: 10.1364/OE.25.010622

    Other URL: http://orcid.org/0000-0002-0289-4680

  • Molecular dynamics in an optical trap of glutamate receptors labeled with quantum-dots on living neurons Reviewed

    Tatsunori Kishimoto, Yasuyo Maezawa, Suguru N. Kudoh, Takahisa Taguchi, Chie Hosokawa

    Proceedings of SPIE - The International Society for Optical Engineering   10252   1025208   2017.04

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    DOI: 10.1117/12.2275026

    Other URL: http://orcid.org/0000-0002-0289-4680

  • Multiplex communication by BP learning in neural network Reviewed

    Shinichi Tamura, Yoshi Nishitani, Chie Hosokawa, Tomomitsu Miyoshi, Hajime Sawai, Yuko Mizuno-Matsumoto, Yen Wei Chen

    Proceedings - 2016 9th International Congress on Image and Signal Processing, BioMedical Engineering and Informatics, CISP-BMEI 2016   825 - 828   2017.02( ISBN:9781509037100

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    © 2016 IEEE. It is a mystery that neural network composed of neurons with fluctuating characteristics can transmit information well reliably. In this paper, we show, in a simulation using a 9×9 2D mesh neural network, 9 to 1 multiplex communication is possible with 99% correct rate. Neurons are modeled by integrate and fire model without leak. Spikes spreads from transmitting neuron groups, propagated as spike waves, and received by receiving neurons. Then, the receiving neurons classify from which neuron group the spike waves come by back propagation neural network (BPN) method.

    DOI: 10.1109/CISP-BMEI.2016.7852824

  • Classification of Spike Wave Propagations in a Cultured Neuronal Network: Investigating a Brain Communication Mechanism Reviewed

    Yoshi Nishitani, Chie Hosokawa, Yuko Mizuno-Matsumoto, Tomomitsu Miyoshi, Shinichi Tamura

    AIMS Neuroscience   4 ( 1 )   1 - 13   2017.01

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    DOI: 10.3934/Neuroscience.2017.1.1.

  • Feasibility of Multiplex Communication in a 2D Mesh Asynchronous Neural Network with Fluctuations Reviewed

    Shinichi Tamura, Yoshi Nishitani, Chie Hosokawa

    AIMS Neuroscience   3 ( 4 )   385 - 397   2016.10( ISSN:2373-8006

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    DOI: 10.3934/Neuroscience.2016.4.385.

  • Surface plasmon-enhanced optical trapping of quantum-dot-conjugated surface molecules on neurons cultured on a plasmonic chip Reviewed

    Kohei Miyauchi, Keiko Tawa, Suguru N. Kudoh, Takahisa Taguchi, Chie Hosokawa

    Japanese Journal of Applied Physics   55 ( 6 )   06GN04   2016.06( ISSN:00214922

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    © 2016 The Japan Society of Applied Physics.Living neurons in a complex neuronal network communicate with each other through synaptic connections. The molecular dynamics of cell surface molecules localized at synaptic terminals is essential for functional connections via synaptic plasticity in the neuronal network. Here, we demonstrate surface-plasmon-resonance-based optical trapping using a plasmonic chip toward realizing effective manipulation of molecules on the surface of neurons. Surface-plasmon-enhanced optical trapping was evaluated by the fluorescence analysis of nanoparticles suspended in water and neural cell adhesion molecules (NCAMs) labeled with quantum dots (Q-dots) on rat hippocampal neurons. The motion of nanoparticles in water and the molecular dynamics of NCAMs on neuronal cells cultured on a plasmonic chip were constrained at the laser focus more effectively than those on a glass substrate because of the surface plasmon resonance effect.

    DOI: 10.7567/JJAP.55.06GN04

  • Fluorescence microscopy imaging of cells with a plasmonic dish integrally molded Reviewed

    Keiko Tawa, Chisato Sasakawa, Tsuyoshi Fujita, Tsuyoshi Fujita, Kazuyuki Kiyosue, Chie Hosokawa, Junji Nishii, Makoto Oike, Norihiro Kakinuma

    Japanese Journal of Applied Physics   55 ( 3 )   03DF12   2016.03( ISSN:00214922

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    © 2016 The Japan Society of Applied Physics. A plastic dish with a wavelength-scale periodic structure at a bottom panel was integrally molded and coated with thin metal films. The integrally molded dish called plasmonic dish was applied to bioimaging under a fluorescence microscope. On the plasmonic substrate, the enhanced electric field based on a grating-coupled surface plasmon resonance (GC-SPR) can provide an enhanced fluorescence. In this study, two kinds of cells, human embryonic kidney (HEK) cells and neuronal cells, were observed in our plasmonic dish. Fluorescence images of HEK cells were above 10 times brighter than those obtained on a conventional glass-bottomed dish. Neuronal cells were successfully cultured for 10 d on the plasmonic dish integrally molded, and in fluorescence images with transmitted light, a higher contrast was obtained than in epifluorescence images. The plasmonic dish integrally molded, as well as that fabricated by the UV nanoimprint method, was also found to be useful for sensitive bioimaging.

    DOI: 10.7567/JJAP.55.03DF12

  • Base of brain intelligence: Information flow in cultured neuronal networks and its simulation on 2D mesh network Reviewed

    Shinichi Tamura, Yoshi Nishitani, Chie Hosokawa, Yuko Mizuno-Matsumoto, Yen Wei Chen

    2015 12th International Conference on Fuzzy Systems and Knowledge Discovery, FSKD 2015   1747 - 1752   2016.01( ISBN:9781467376822

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    © 2015 IEEE. It's important for understanding brain intelligence to investigate how the signal/information is flown in neuronal network. We observed spike trains obtained by one-shot electrical stimulation with 8 × 8 multi-electrodes in cultured neuronal networks. Each electrode is considered to collect spikes from several neurons. We then constructed code flow maps as movies of the electrode array to observe the code flow especially of 1101 and 1011. To quantify the flow, we calculated the cross-correlations of the maximum direction of the codes with lengths less than 8. Normalized cross-correlations in the maximum direction were almost constant irrespective of code. Thus, the analysis suggested that the local codes for electrode flow maintained the code shape to some extent and conveyed information in the neural network. Then we made simulation of such code flow, and could estimate rough characteristics of neurons including refractory period and distribution of connection weights between neurons.

    DOI: 10.1109/FSKD.2015.7382211

  • Simulation of Code Spectrum and Code Flow of Cultured Neuronal Networks. Reviewed

    Shinichi Tamura,Yoshi Nishitani,Chie Hosokawa,Tomomitsu Miyoshi,Hajime Sawai

    Computational Intelligence and Neuroscience   2016   7186092:1-7186092:12   2016( ISSN:1687-5265

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1155/2016/7186092

    Other URL: http://dblp.uni-trier.de/db/journals/cin/cin2016.html#journals/cin/TamuraNHMS16

  • Spike Code Flow in Cultured Neuronal Networks. Reviewed

    Shinichi Tamura,Yoshi Nishitani,Chie Hosokawa,Tomomitsu Miyoshi,Hajime Sawai,Takuya Kamimura,Yasushi Yagi,Yuko Mizuno-Matsumoto,Yen-Wei Chen

    Computational Intelligence and Neuroscience   2016   7267691:1-7267691:11   2016( ISSN:1687-5265

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1155/2016/7267691

    Other URL: http://dblp.uni-trier.de/db/journals/cin/cin2016.html#journals/cin/TamuraNHMSKYMC16

  • feasibility of multiplex communication in a 2D mesh asynchronous neural network with fluctuations Reviewed

    Shinichi Tamura, Yoshi Nishitani, Chie Hosokawa

    AIMS Neuroscience   3 ( 4 )   385 - 397   2016( ISSN:2373-7972

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    Publishing type:Research paper (scientific journal)  

    © 2016 Shinichi Tamura et al. It remains a mystery how neural networks composed of neurons with fluctuating characteristics can reliably transmit information. In this study, we simulated a 9 × 9 2D mesh neural network consisting of an integrate-and-fire model without leak, and connection weights that were randomly generated. The characteristics of the refractory period and output delay of the neurons were fluctuated time to time. Spikes from transmitting neuron groups spread (propagated as spike waves) to receiving neurons. For 9 to 1 multiplex communication with a back propagation neural network (BPN), the receiving neurons successfully classified which neuron group transmitted the spike at a rate of 99%. In other words, the activity of the neuron group is propagated in the neural network as spike waves in a broadcasting manner and the wave fragment is received by receiving neurons. Next, point-to-point signal transmission in the neural network is carried out by multi-path, multiplex communication, and diversity reception. Each neuron can function in 3 ways of transmit, relay (transfer), and receive; however, most neurons act as a local relaying media. This type of mechanism is similar to sound propagation through air. Our research group studies the functions of neural networks by combining experiments with cultured neuronal networks with artificial neural network simulations. This current study corresponding to our previous work on the ability of remote receiving neurons to identify two transmitting neuron groups stimulated in a cultured neuronal network, i.e., 2 to 1 communication. These mechanisms may be the basis of higher cortical functions.

    DOI: 10.3934/Neuroscience.2016.4.385

  • Variance of spatiotemporal spiking patterns by different stimulated neurons in cultured neuronal networks Reviewed

    Yoshi Nishitani, Chie Hosokawa, Yuko Mizuno-Matsumoto, Tomomitsu Miyoshi, Hajime Sawai, Shinichi Tamura

    International Journal of Academic Research and Reflection   4 ( 3 )   11 - 19   2016( ISSN:2309-0405

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  • Spike code and information flow in cultured neuronal networks and its simulation on 2D mesh network Reviewed

    Shinichi Tamura, Yoshi Nishitani, Chie Hosokawa, Yuko Mizuno-Matsumoto

    Proceedings of the 2015 7th IEEE International Conference on Cybernetics and Intelligent Systems, CIS 2015 and Robotics, Automation and Mechatronics, RAM 2015   191 - 195   2015.09( ISBN:9781467373364

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    Publishing type:Research paper (international conference proceedings)  

    © 2015 IEEE. It's important to investigate natural intelligence how the signal/ information is flown in neuronal network. We observed spike trains produced by one-shot electrical stimulation with 8 × 8 multi-electrodes in cultured neuronal networks. Each electrode accepted spikes from several neurons. We then constructed code flow maps as movies of the electrode array to observe the code flow especially of '1101' and '1011.' To quantify the flow, we calculated the cross-correlations of the maximum direction of the codes with lengths less than 8. Normalized cross-correlations in the maximum direction were almost constant irrespective of code. Thus, the analysis suggested that the local codes for electrode flow maintained the code shape to some extent and conveyed information in the neural network. Then we made simulation of such code flow.

    DOI: 10.1109/ICCIS.2015.7274571

  • Base of brain intelligence: Information flow in cultured neuronal networks and its simulation on 2D mesh network. Reviewed

    Shinichi Tamura,Yoshi Nishitani,Chie Hosokawa,Yuko Mizuno-Matsumoto,Yen-Wei Chen

    IEEE Proceedings of 12th International Conference on Fuzzy Systems and Knowledge Discovery   1747 - 1752   2015.08

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    DOI: 10.1109/FSKD.2015.7382211

    Other URL: http://dblp.uni-trier.de/db/conf/fskd/fskd2015.html#conf/fskd/TamuraNHMC15

  • Laser-induced perturbation into molecular dynamics localized in neuronal cell Reviewed

    Hosokawa Chie, Takeda Naoko, Kudoh Suguru N., Taguchi Takahisa

    Proceedings of SPIE - The International Society for Optical Engineering   9305   93052N   2015.01( ISSN:0277-786X

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    DOI: 10.1117/12.2076348

  • In situ sensitive fluorescence imaging of neurons cultured on a plasmonic dish using fluorescence microscopy Reviewed

    Keiko Tawa, Chikara Yasui, Chie Hosokawa, Hiroyuki Aota, Junji Nishii

    ACS Applied Materials and Interfaces   6 ( 22 )   20010 - 20015   2014.11( ISSN:1944-8244

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    © 2014 American Chemical Society. A plasmonic dish was fabricated as a novel cellculture dish for in situ sensitive imaging applications, in which the cover glass of a glass-bottomed dish was replaced by a grating substrate coated with a film of silver. Neuronal cells were successfully cultured over a period of more than 2 weeks in the plasmonic dish. The fluorescence images of their cells including dendrites were simply observed in situ using a conventional fluorescence microscope. The fluorescence from neuronal cells growing along the dish surface was enhanced using the surface plasmon resonance field. Under an epi-fluorescence microscope and employing a donut-type pinhole, the fluorescence intensity of the neuron dendrites was found to be enhanced efficiently by an order of magnitude compared with that using a conventional glass-bottomed dish. In a transmitted-light fluorescence microscope, the surface-selective fluorescence image of a fine dendrite growing along the dish surface was observed; therefore, the spatial resolution was improved compared with the epi-fluorescence image of the identical dendrite. (Chemical Equation Presented).

    DOI: 10.1021/am505579u

    PubMed

  • Bright fluorescence imaging of cells cultured on the plasmonic dish Reviewed

    Keiko Tawa, Tsuyoshi Fujita, Chikara Yasui, Kazuyuki Kiyosue, Chie Hosokawa, Chisato Sasakawa, Hiroyuki Aota, Norihiro Kakinuma, Makoto Oike, Junji Nishii

    AMER CHEMICAL SOC ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY   248   2014.08( ISSN:0065-7727

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    Other URL: http://orcid.org/0000-0002-5736-1187

  • Trapping of Neural Cell Adhesion Molecules in Neurons with Resonant Optical Tweezers Reviewed

    TAKEDA NAOKO, KUDO SUGURU, TAGUCHI TAKAHISA, HOSOKAWA CHIE

    電気学会論文誌 C   134 ( 8 )   1071 - 1077   2014( ISSN:0385-4221

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    DOI: 10.1541/ieejeiss.134.1071

    J-GLOBAL

  • Bright fluorescence imaging of cells cultured on the plasmonic dish

    Tawa Keiko, Fujita Tsuyoshi, Yasui Chikara, Kiyosue Kazuyuki, Hosokawa Chie, Sasakawa Chisato, Aota Hiroyuki, Kakinuma Norihiro, Oike Makoto, Nishii Junji

    Abstracts of Papers of the American Chemical Society   248   2014

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    Publishing type:Research paper (other academic)   Kind of work:Joint Work  

    Other URL: http://orcid.org/0000-0002-0289-4680

  • Synchronized code sequences from spike trains in cultured neuronal networks Reviewed

    Nishitani Y, Hosokawa C, Mizuno-Matsumoto Y, Miyoshi T, Sawai H, Tamura S

    International Journal of Engineering and Industries   5 ( 3 )   12 - 24   2014

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  • An influence of bit timing function in neuron spike trains on M-sequence detection Reviewed

    Yoshi Nishitani, Chie Hosokawa, Yuko Mizuno-Matsumoto, Tomomitsu Miyoshi, Hajime Sawai, Shinichi Tamura

    Journal of Global Business Society   2 ( 1 )   51 - 56   2014

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  • Trapping of Neural Cell Adhesion Molecules in Neurons with Resonant Optical Tweezers Reviewed

    武田尚子, 武田尚子, 工藤卓, 田口隆久, 細川千絵

    電気学会論文誌 C   134 ( 8 )   1071 - 1077   2014( ISSN:0385-4221

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    © 2014 The Institute of Electrical Engineers of Japan. Living neuronal network in neural systems communicate through synaptic connections. For aiming artificial control of the synaptic transmission in neuronal network, we have applied conventional optical tweezers using a focused near-infrared (NIR) laser. The optical trapping of neural cell adhesion molecules (NCAMs) labeled with quantum-dots (Q-dots) at the plasma membrane of living neuronal cells was achieved at high laser power (> 300 mW) in our previous study; therefore, the further reduction of laser power was required. Here, we propose and demonstrate resonant optical tweezers of Q-dots attached to NCAM in a neuron by simultaneous irradiation with non-resonance and resonance laser beams. The optical trapping dynamics of them was revealed by fluorescence correlation spectroscopy (FCS). The transit times of Q-dots attached to NCAM at the focal spot increased with the simultaneous irradiation of NIR laser and resonance laser. These results suggest that the particle motion of Q-dots attached to NCAM was constrained at the focus spot due to optical trapping force enhanced with resonance laser irradiation. Our method is applicable to selective manipulation of signaling molecules in living neurons.

    DOI: 10.1541/ieejeiss.134.1071

    J-GLOBAL

  • Synchronized code sequences from spike trains in cultured neuronal networks Reviewed

    Nishitani Y, Hosokawa C, Mizuno-Matsumoto Y, Miyoshi T, Sawai H, Tamura S

    International Journal of Engineering and Industries   5 ( 3 )   12 - 24   2014

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  • Multiplexed Spatiotemporal Communication Model in Artificial Neural Networks Reviewed

    Tamura S, Nishitani Y, Kamimura T, Yagi Y, Hosokawa C, Miyoshi T, Sawai H, Mizuno-Matsumoto Y, Chen Y-W

    Automation, Control and Intelligent Systems   1 ( 6 )   121 - 130   2013.12

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    DOI: 10.11648/j.acis.20130106.11

  • Laser manipulation of intracellular molecular dynamics in hippocampal neurons Reviewed

    C. Hosokawa, Y. Ueda, N. Takeda, S. N. Kudoh, and T. Taguchi

    IEEE Proceedings of Pacific Rim Conference on Lasers and Electro-Optics   WJ3   2013.09

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    DOI: 10.1109/CLEOPR.2013.6600270

  • Femtosecond laser-induced stimulation of a single neuron in a neuronal network Reviewed

    Chie Hosokawa, Yasutaka Sakamoto, Suguru N. Kudoh, Yoichiroh Hosokawa, Takahisa Taguchi

    Applied Physics A: Materials Science and Processing   110 ( 3 )   607 - 612   2013.03( ISSN:0947-8396

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    We demonstrated the stimulation of neurons at a single-cell level in cultured neuronal network by a focused femtosecond laser. When the femtosecond laser was focused on a neuron loaded with a fluorescent calcium indicator, the fluorescence intensity immediately increased at the laser spot, suggesting that intracellular Ca2+ increases in the neuronal cell due to the femtosecond laser irradiation. The probability of Ca2+ elevation at the laser spot depended on the average laser power, irradiation time, and position of the focal point along the optical axis, indicating that the femtosecond laser activates neurons because of multiphoton absorption. Moreover, after laser irradiation of a single neuron cultured on multielectrode arrays, the evoked electrical activity of the neurons was demonstrated by electrophysiological systems, which concluded that the focused femtosecond laser could achieve stimulating a single neuron in a neuronal network with high spatial and temporal resolution. © Springer-Verlag 2012.

    DOI: 10.1007/s00339-012-7137-6

    Other URL: http://orcid.org/0000-0002-0289-4680

  • Clear images of neuronal cells cultured on a plasmonic dish observed with the inverted fluorescence microscope Reviewed

    Keiko Tawa, Tsuyoshi Fujita, Chie Hosokawa, Junji Nishii

    Optics InfoBase Conference Papers   2013

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    DOI: 10.1364/jsap.2013.16p_d5_8

  • Laser manipulation of intracellular molecular dynamics in hippocampal neurons Reviewed

    C. Hosokawa, Y. Ueda, N. Takeda, S. N. Kudoh, T. Taguchi

    Pacific Rim Conference on Lasers and Electro-Optics, CLEO - Technical Digest   WJ3   2013( ISBN:9781467364751

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    Publishing type:Research paper (international conference proceedings)  

    We demonstrated laser manipulation of intracellular molecular dynamics in neurons to realize artificial control toward modulating synaptic transmission in a neuronal network. The optical trapping and assembling dynamics of synaptic vesicles and neural cell adhesion molecules in neurons was revealed by fluorescence analysis. © 2013 IEEE.

    DOI: 10.1109/CLEOPR.2013.6600270

  • Carbon nanotube-liposome supramolecular nanotrains for intelligent molecular-transport systems. Reviewed

    Miyako E, Kono K, Yuba E, Hosokawa C, Nagai H, Hagihara Y

    Nature communications   3   1226   2012.11( ISSN:2041-1723

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1038/ncomms2233

    PubMed

  • M-Sequence Family from Cultured Neural Circuits Reviewed

    Tamura Shinichi, Nishitani Yoshi, Hosokawa Chie, Mizuno-Matsumoto Yuko, Kamimura Takuya, Chen Yen-Wei, Miyoshi Tomomitsu, Sawai Hajime

    IEEE Proceedings of 6TH INTERNATIONAL CONFERENCE ON NEW TRENDS IN INFORMATION SCIENCE, SERVICE SCIENCE AND DATA MINING (ISSDM2012)   466 - 471   2012.10

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    Publishing type:Research paper (international conference proceedings)   Kind of work:Joint Work  

  • Sensitive Fluorescence Microscopy of Neurons Cultured on a Plasmonic Chip Reviewed

    YASUI Chikara,TAWA Keiko,HOSOKAWA Chie,NISHII Junji,AOTA Hiroyuki,MATSUMOTO Akira

    Japanese Journal of Applied Physics   51 ( 6,Issue 2 )   06FK10.1-06FK10.5   2012.06( ISSN:0021-4922

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1143/JJAP.51.06FK10

    J-GLOBAL

  • Electroluminescence of Hybrid Self-Organised Fibres Incorporating CdTe Quantum Dots Reviewed

    ANDO Masanori,HOSOKAWA Chie,YANG Ping,MURASE Norio

    Australian Journal of Chemistry   65 ( 9 )   1257 - 1259   2012( ISSN:0004-9425

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1071/CH12127

  • Detection of M-sequences from spike sequence in neuronal networks Reviewed

    Yoshi Nishitani, Chie Hosokawa, Yuko Mizuno-Matsumoto, Tomomitsu Miyoshi, Hajime Sawai, Shinichi Tamura

    Computational Intelligence and Neuroscience   2012   862579   2012( ISSN:1687-5265

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    Publishing type:Research paper (scientific journal)  

    In circuit theory, it is well known that a linear feedback shift register (LFSR) circuit generates pseudorandom bit sequences (PRBS), including an M-sequence with the maximum period of length. In this study, we tried to detect M-sequences known as a pseudorandom sequence generated by the LFSR circuit from time series patterns of stimulated action potentials. Stimulated action potentials were recorded from dissociated cultures of hippocampal neurons grown on a multielectrode array. We could find several M-sequences from a 3-stage LFSR circuit (M3). These results show the possibility of assembling LFSR circuits or its equivalent ones in a neuronal network. However, since the M3 pattern was composed of only four spike intervals, the possibility of an accidental detection was not zero. Then, we detected M-sequences from random spike sequences which were not generated from an LFSR circuit and compare the result with the number of M-sequences from the originally observed raster data. As a result, a significant difference was confirmed: a greater number of 01 reversed the 3-stage M-sequences occurred than would have accidentally be detected. This result suggests that some LFSR equivalent circuits are assembled in neuronal networks. © Copyright 2012 Yoshi Nishitani et al.

    DOI: 10.1155/2012/862579

    PubMed

  • A photo-thermal-electrical converter based on carbon nanotubes for bioelectronic applications Reviewed

    Eijiro Miyako, Chie Hosokawa, Masami Kojima, Masako Yudasaka, Ryoji Funahashi, Isao Oishi, Yoshihisa Hagihara, Mototada Shichiri, Mizuki Takashima, Keiko Nishio, Yasukazu Yoshida

    Angewandte Chemie - International Edition   50 ( 51 )   12266 - 12270   2011.12( ISSN:1433-7851

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    Publishing type:Research paper (scientific journal)  

    Electrifying! A device based on carbon nanotubes wrapped with poly(3-hexylthiophene) (and dispersed in poly(dimethylsiloxane)) sheets can effectively convert laser light into thermal energy and subsequently to electricity. The converter is flexible and extremely compact (see picture), and can be manipulated by using a laser that functions in the wavelength range that can be transmitted through living tissue. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

    DOI: 10.1002/anie.201106136

    PubMed

  • Optical trapping of synaptic vesicles in neurons Reviewed

    HOSOKAWA Chie,KUDOH Suguru N.,KIYOHARA Ai,TAGUCHI Takahisa

    Applied Physics Letters   98 ( 16 )   163705   2011.04( ISSN:0003-6951

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1063/1.3579191

  • Vitroid - The robot system with an interface between a living neuronal network and outer world Reviewed

    Suguru N. Kudoh, Minori Tokuda, Ai Kiyohara, Chie Hosokawa, Takahisa Taguchi, Isao Hayashi

    International Journal of Mechatronics and Manufacturing Systems   4 ( 2 )   135 - 149   2011.03( ISSN:1753-1039

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    Publishing type:Research paper (scientific journal)  

    We have developed a neuro-robot-hybrid system using a living neuronal network and a miniature moving robot. The living network of rat hippocampal neurons can distinguish patterns of action potentials evoked by different inputs, suggesting that a cultured neuronal network can represent particular states as symbols. We used a Khepera II robot and a robot made using a LEGO mindstorm NXT kit to interface with a living neuronal network and the outer world. We call the system 'vitroid'. Vitroid has living neurons, a robot body, and direct coupling controllers to interface the neurons with the robot. Vitroid was able to perform obstacle avoidance behaviour with premised control rule sets. Copyright © 2011 Inderscience Enterprises Ltd.

    DOI: 10.1504/IJMMS.2011.039264

  • Optical stimulation of hippocampal neurons by a focused femtosecond laser Reviewed

    Sakamoto Yasutaka, Hosokawa Chie, Kudoh Suguru N., Taguchi Takahisa

    NEUROSCIENCE RESEARCH   71   E206 - E207   2011( ISSN:0168-0102

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    Publishing type:Research paper (international conference proceedings)   Kind of work:Joint Work  

    DOI: 10.1016/j.neures.2011.07.894

  • Micro-channel fabrication by femtosecond laser to arrange neuronal cells on multi-electrode arrays Reviewed

    Chie Hosokawa, Suguru N. Kudoh, Mariko Suzuki, Ai Kiyohara, Yoichiroh Hosokawa, Kazunori Okano, Hiroshi Masuhara, Takahisa Taguchi

    Applied Physics A: Materials Science and Processing   101 ( 2 )   423 - 428   2010.11( ISSN:0947-8396

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    Publishing type:Research paper (scientific journal)  

    We propose and demonstrate micro-channel fabrication by femtosecond-laser processing to arrange neuronal cells on multi-electrode arrays (MEAs). When neuronal cells were seeded in the micro-channels on MEAs, they elongated their neurites along the channel and finally formed the network patterned on MEAs. The spontaneous electrical activity of the neuronal networks on MEAs was evaluated by using electrophysiological systems, indicating that patterned network was functionally activated along the channels. The present method is applicable to analyze network dynamics of living neuronal cells patterned artificially. © 2010 Springer-Verlag.

    DOI: 10.1007/s00339-010-5836-4

    CiNii Article

  • Bright, non-blinking, and less-cytotoxic SiO2 beads with multiple CdSe/ZnS nanocrystals Reviewed

    Ping Yang, Norio Murase, Mariko Suzuki, Chie Hosokawa, Kazunori Kawasaki, Tomoki Kato, Takahisa Taguchi

    Chemical Communications   46 ( 25 )   4595 - 4597   2010.07( ISSN:1359-7345

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    Publishing type:Research paper (scientific journal)  

    A method including surface silanization, phase transfer and self-assembly, and SiO2 shell growth has been developed to incorporate multiple hydrophobic CdSe/ZnS nanocrystals into SiO2 beads where they are well suited for bio-application due to their high brightness, less-cytotoxic, and non-blinking nature. © 2010 The Royal Society of Chemistry.

    DOI: 10.1039/c002243h

    PubMed

    CiNii Article

  • Fluorescence analysis of optical trapping dynamics of synaptic vesicles in dissociated neuron Reviewed

    Hosokawa Chie, Onishi Eriko, Kudoh Suguru N., Taguchi Takahisa

    NEUROSCIENCE RESEARCH   68   E234   2010( ISSN:0168-0102

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    Publishing type:Research paper (international conference proceedings)   Kind of work:Joint Work  

    DOI: 10.1016/j.neures.2010.07.1034

  • Memory on a chip Reviewed

    Hidekatsu Ito, Chie Hosokawa, Suguru N. Kudoh

    2010 International Symposium on Micro-NanoMechatronics and Human Science: From Micro and Nano Scale Systems to Robotics and Mechatronics Systems, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010   121 - 126   2010( ISBN:9781424479962

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    Publishing type:Research paper (international conference proceedings)  

    The rat hippocampal neurons were cultured on a dish with 64 planer micro electrodes array. Neurons reorganized a functional network, and an external inputs form outer world elicited a reproducible, particular spatiotemporal pattern of evoked action potentials. The patterns were not completely uniform for repeptive stimulation but varied slightly. We previously reported that dissociated rat hippocampal neurons possessed hysterical function in its network dynamics. The spatio-temporal electrical activity of the living neuronal network evoked by paired stimulation is quite different from one evoked by a single stimulation, suggested that the network can "memorize" an existence of precedent stimulation. In this report, the feature of this type of network hysteresis was investigated and the hysteresis was kept for at least 1s and not kept for 10s. In addition, this hysteresis was remarkable only in aged cultures with matured complex network. Network hysteresis depends on internal state of the network, generated by the autonomous activity in self-organized networks. ©2010 IEEE.

    DOI: 10.1109/MHS.2010.5669566

  • Femtosecond laser modification of living neuronal network Reviewed

    Chie Hosokawa, Suguru N. Kudoh, Ai Kiyohara, Yoichiroh Hosokawa, Kazunori Okano, Hiroshi Masuhara, Takahisa Taguchi

    Applied Physics A: Materials Science and Processing   93 ( 1 )   57 - 63   2008.10( ISSN:0947-8396

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    Publishing type:Research paper (scientific journal)  

    An all-wet femtosecond laser microprocessing technique was utilized for patterning and cutting functional network of living neuronal cells on a multi-electrode dish (MED). The neuronal cells cultured on a source substrate were transferred onto an electrode in a MED probe in solution by utilizing a femtosecond laser-induced impulsive force and a pattern of neuronal cells were formed on the MED probe. The cellular activity of the detached neurons was supported that neurites could be regenerated around the electrodes. As another processing method, the neurons stretching between electrodes were selectively cut by the direct femtosecond laser irradiation and the spontaneous electrical activity of the neuronal network was evaluated. While the spontaneous action potentials of neurons were synchronized before the cutting, the synchronization disappeared after the cutting, indicating that the neuronal network is locally disconnected by the laser cutting. The present method is applicable to artificial reconstruction of living neuronal network. © 2008 Springer-Verlag.

    DOI: 10.1007/s00339-008-4655-3

  • Resynchronization in neuronal network divided by femtosecond laser processing. Reviewed

    Hosokawa C, Kudoh SN, Kiyohara A, Taguchi T

    Neuroreport   19 ( 7 )   771 - 775   2008.05( ISSN:0959-4965

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1097/WNR.0b013e3282fdf6b3

    PubMed

  • Vitroid - a robot with link between living neuronal network in vitro and robot body Reviewed

    Suguru N. Kudoh, Minori Tokuda, Ai Kiyohara, Chie Hosokawa, Takahisa Taguchi, Isao Hayashi

    Proceedings of 2008 IEEE International Conference on Mechatronics and Automation, ICMA 2008   375 - 378   2008( ISBN:9781424426324

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    Publishing type:Research paper (international conference proceedings)  

    Rat hippocampal neurons organized complex networks on a culture dish which has 64 planar microelectrodes and the spontaneous action potentials were frequently observed. The living neuronal network was able to distinguish patterns of action potentials evoked by different inputs, suggesting that a cultured neuronal network can represent and process particular states as symbols. We use a Khepera II robot and a robot made by LEGO mindstorm NXT kit as a robot body for interfacing with a living neuronal network and the outer world. We call the system "Vitroid". Vitroid has the living neurons, robot body, and direct coupling type of controllers to interface neurons with the robot. We succeeded in performing obstacle avoidance behavior with premised control rule sets. Using self-tuning fuzzy reasoning, we associated a distinct spatial pattern of electrical activity with a particular phenomenon in the outside of the culture dish. © 2008 IEEE.

    DOI: 10.1109/ICMA.2008.4798783

  • Photon force-induced phase transition dynamics of single hydrogel nanoparticles in water Reviewed

    C. Hosokawa, C. Hosokawa, Y. Matsumura, Y. Matsumura, H. Yoshikawa, K. Iwai, H. Masuhara, H. Masuhara

    Acta Physica Polonica A   112   S57 - S70   2007.11( ISSN:05874246

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    We investigated laser-induced phase transition dynamics of μm sized single poly(N-isopropylacrylamide) hydrogel particles in water. Single poly(N--isopropylacrylamide) gel particles labeled with a polarity-sensitive fluorescent probe monomer were optically trapped by a focused laser beam and its fluorescence dynamics was analyzed. The fluorescence intensity of trapped single gel particles was increased with the trapping laser power, while the fluorescence peak wavelength was not changed. Temperature-induced changes of fluorescence properties of the single particle were confirmed to be similar to those of the bulk solution. These behaviors are well interpreted by considering that the fluorescence intensity and fluorescence peak reflect local interactions between the fluorescent probe and attaching (bound) water molecules and effective polarity determined by (free) water content in the particle, respectively. A change in the fluorescence peak wavelength after laser trapping was followed and its blue-shift was confirmed to occur within a few hundreds seconds, indicating that a single gel particle gradually attains to a globular state on this timescale, expelling initially free water molecules and then bound ones.

  • Biomodeling System - Interaction Between Living Neuronal Networks and the Outer World. Reviewed

    Suguru N. Kudoh,Chie Hosokawa,Ai Kiyohara,Takahisa Taguchi,Isao Hayashi

    Journal of Robotics and Mechatronics   19 ( 5 )   592 - 600   2007

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    Other URL: http://dblp.uni-trier.de/db/journals/jrm/jrm19.html#journals/jrm/KudohHKTH07

  • Functional regeneration of hippocampal neurons on multielectrode array dish controlled by femtosecond laser processing Reviewed

    Hosokawa Chie, Kiyohara Ai, Kudoh Suguru N., Taguchi Takahisa

    NEUROSCIENCE RESEARCH   58   S147   2007( ISSN:0168-0102

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    Publishing type:Research paper (international conference proceedings)   Kind of work:Joint Work  

    DOI: 10.1016/j.neures.2007.06.1433

  • Interaction between living neuronal network and outer world by programmable multisite stimulation system Reviewed

    S. N. Kudoh, A. Kiyohara, C. Hosokawa, T. Taguchi, I. Hayashi

    IEEE Proceedings of 2007 INTERNATIONAL SYMPOSIUM ON MICRO-NANO MECHATRONICS AND HUMAN SCIENCE   44 - 49   2007( ISSN:2474-378X

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    Publishing type:Research paper (international conference proceedings)   Kind of work:Joint Work  

    DOI: 10.1109/MHS.2007.4420824

  • Enhancement of biased diffusion of dye-doped nanopartieles by simultaneous irradiation with resonance and nonresonance laser beams Reviewed

    Chie Hosokawa, Hiroyuki Yoshikawa, Hiroshi Masuhara

    Japanese Journal of Applied Physics, Part 2: Letters   45   L453 - L456   2006.05( ISSN:00214922

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    We propose and demonstrate the enhancement of the biased diffusion of dye-doped nanoparticles using resonance and nonresonance laser beams. The Brownian motion of nanoparticles in a laser focus is investigated by fluorescence correlation spectroscopy (PCS) and the time variation in fluorescence intensity. From the analysis of autocorrelation functions, it is demonstrated that the difference between the transit times of nanoparticles in the focal spot with and without resonance laser irradiation increases ∼7-fold by the simultaneous irradiation of a near-infrared laser. This method is applicable to the selective optical manipulation of dye-stained nanomaterials and biomolecules in solution. © 2006 The Japan Society of Applied Physics.

    DOI: 10.1143/JJAP.45.L453

  • Optical trapping and fluorescence analysis of thermo-responsive poly(N-alkylacrylamide) microgel particles in water Reviewed

    Yuriko Matsumura, Chie Hosokawa, Hiroyuki Yoshikawa, Kaoru Iwai, Hiroshi Masuhara

    Polymer Preprints, Japan   55 ( 1 )   1750   2006

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    Publishing type:Research paper (scientific journal)  

    When laser beam was focused into nanoparticle suspensions, nanoparticles can be trapped in the focal spot due to optical gradient force. Here we report the optical trapping and fluorescence analysis of poly(N-isopropylacrylamide) (PNIPAM) and its related polymer microgel particles which were labeled with 4-N-(2-acryloyloxyethyl)-N-methylamino-7-N,N-dimethylaminosulfonyl-2,1, 3-benzoxadiazole (DBD-AE) dispersed in water. The DBD-AE labeled PNIPAM microgel particles were trapped at the focal point by irradiation with cw-Nd 3+:YAG laser (λ = 1064 nm) in water and the fluorescence intensity of the DBD-AE labeled PNIPAM microgel particles excited by diode laser (λ = 405 nm) increased stepwise. We succeeded to fluorescence analysis of trapped single microgel particle.

  • Cluster formation of nanoparticles in an optical trap studied by fluorescence correlation spectroscopy Reviewed

    HOSOKAWA Chie,YOSHIKAWA Hiroyuki,MASUHARA Hiroshi

    Phys Rev E Stat Nonlinear Soft Matter Phys   72 ( 2,Pt.1 )   021408   2005.08( ISSN:1539-3755

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    Publishing type:Research paper (scientific journal)   Kind of work:Joint Work  

    DOI: 10.1103/PhysRevE.72.021408

  • Cluster formation of nanoparticles in an optical trap studied by fluorescence correlation spectroscopy Reviewed

    Chie Hosokawa, Hiroyuki Yoshikawa, Hiroshi Masuhara

    Physical Review E - Statistical, Nonlinear, and Soft Matter Physics   72 ( 2 )   021408   2005.08( ISSN:1539-3755

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    Publishing type:Research paper (scientific journal)  

    We report in situ observation of cluster growth of nanoparticles confined in an optical trapping potential by means of fluorescence correlation spectroscopy. When an optical trapping force caused by a highly focused laser beam acts on nanoparticle suspensions, the number of nanoparticles increases and an assembly can be formed at the focal spot. The decay times of fluorescence autocorrelation curves were investigated as a function of the irradiation time of the laser beam and the laser power. In the initial stage of the optical assembling, the decay time increases with the irradiation time of the laser beam. On the other hand, in the later stage, a decrease of the decay time was observed. This behavior is explained successfully by using two models of Brownian motion under weak and strong optical trapping. It was revealed that trapping and clustering of nanoparticles proceed simultaneously and clusters confined in the focal spot make larger aggregates spontaneously. © 2005 The American Physical Society.

    DOI: 10.1103/PhysRevE.72.021408

    PubMed

  • Secondary structure and secondary structure dynamics of DNA hairpins complexed with HIV-1 NC protein. Reviewed

    Gonzalo Cosa, Elizabeth J. Harbron, Yining Zeng, Hsiao Wei Liu, Donald B. O'Connor, Chie Eta-Hosokawa, Karin Musier-Forsyth, Paul F. Barbara

    Biophysical journal   87 ( 4 )   2759 - 2767   2004.10( ISSN:0006-3495

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    Publishing type:Research paper (scientific journal)  

    Reverse transcription of the HIV-1 RNA genome involves several complex nucleic acid rearrangement steps that are catalyzed by the HIV-1 nucleocapsid protein (NC), including for example, the annealing of the transactivation response (TAR) region of the viral RNA to the complementary region (TAR DNA) in minus-strand strong-stop DNA. We report herein single-molecule fluorescence resonance energy transfer measurements on single immobilized TAR DNA hairpins and hairpin mutants complexed with NC (i.e., TAR DNA/NC). Using this approach we have explored the conformational distribution and dynamics of the hairpins in the presence and absence of NC protein. The data demonstrate that NC shifts the equilibrium secondary structure of TAR DNA hairpins from a fully "closed" conformation to essentially one specific "partially open" conformation. In this specific conformation, the two terminal stems are "open" or unwound and the other stems are closed. This partially open conformation is arguably a key TAR DNA intermediate in the NC-induced annealing mechanism of TAR DNA. Copyright 2004 Biophysical Society

    DOI: 10.1529/biophysj.104.043083

    PubMed

  • Optical assembling dynamics of individual polymer nanospheres investigated by single-particle fluorescence detection Reviewed

    Chie Hosokawa, Hiroyuki Yoshikawa, Hiroshi Masuhara

    Physical Review E - Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics   70 ( 6 )   7   2004( ISSN:1063-651X

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    Publishing type:Research paper (scientific journal)  

    When a laser beam is focused into colloidal nanoparticle suspensions, a number of nanoparticles can be confined in the focal spot due to an optical gradient force. To reveal the assembling dynamics of polymer nanoparticles, the assembling process was investigated by analyzing the time evolution of the fluorescence intensity of the nanoparticles. In a dilute suspension of 100-nm-sized particles, a stepwise increase of the fluorescence intensity corresponding to a trapped single nanoparticle was observed. Statistical analysis revealed that the initial assembling rate of nanoparticles was proportional to the laser power and concentration of particle suspensions as expected from the diffusion equation. In 40-nm-sized particle suspensions, blinking profiles of fluorescence intensity were obtained, in which 2–3 particles were simultaneously trapped and then escaped from the focal point. It is considered from statistical analyses and two-dimensional Monte Carlo simulations that this assembling phenomenon is attributable to cluster formation assisted by optical trapping. © 2004 The American Physical Society.

    DOI: 10.1103/PhysRevE.70.061410

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Books and Other Publications

  • 光圧 : 物質制御のための新しい光利用

    石原 一 , 芦田 昌明( Role: Joint author ,  プラズモニックチップを用いたバイオイメージング)

    朝倉書店  2021.06  ( ISBN:9784759813920

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    Total pages:207   Responsible for pages:184‐195   Book type:Scholarly book

  • プラズモンと光圧が導くナノ物質科学(CSJ Current Review)

    田和圭子, 細川千絵( Role: Sole author ,  プラズモニックチップを用いたバイオイメージング)

    2019.03  ( ISBN:9784759813920

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    Total pages:216   Responsible for pages:79‐86   Book type:Scholarly book

  • Nanophotonics

    H. Yoshikawa, C. Hosokawa, H. Masuhara( Role: Joint author ,  Chapter 15 Optical trapping and assembling of nanoparticles)

    Elsevier  2006.01  ( ISBN:9780444522498

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    Total pages:316   Responsible for pages:275-288   Book type:Scholarly book

MISC

  • Manipulation of cellular functions in neuronal networks with optical perturbation of a focused laser beam Reviewed

    HOSOKAWA Chie

    Oyo Buturi   92 ( 3 )   166 - 170   2023.03( ISSN:03698009 ( eISSN:21882290

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    <p>Neurons in complex networks communicate with each other through synaptic connections. Molecular dynamics at synaptic terminals in neurons is essential for synaptic plasticity and subsequent modulation of cellular functions in neuronal networks. For realizing artificial control of living neurons, we propose and demonstrate laser-induced perturbation into molecular dynamics in neuronal cells. The optical trapping of cellular molecules such as synaptic vesicles or neurotransmitter receptors labeled with quantum-dots is evaluated by fluorescence analysis. Moreover, we apply femtosecond laser-induced stimulation to evaluate the spatiotemporal activity patterns in cultured neuronal networks. These methods have the potential to modulate synaptic transmission of a particular neuron in neuronal networks without any drugs and genes.</p>

    DOI: 10.11470/oubutsu.92.3_166

  • Molecular Manipulation of Neurons by Optical Trapping with a Focused Laser Beam Reviewed

    Chie Hosokawa

    レーザー研究   50 ( 2 )   82 - 86   2022( ISSN:0387-0200

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    Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    J-GLOBAL

  • レーザー摂動による細胞機能操作 Reviewed

    Chie Hosokawa

    Molecular Electronics and Bioelectronics (Web)   33 ( 2 )   45 - 48   2022( ISSN:2423-8805

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    Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    J-GLOBAL

  • Analysis of a fluid flow in optical trapping of tiny particle Reviewed

    TSUJI Tetsuro, HOSOKAWA Chie, KISHIMOTO Tatsunori, OKUBO Takumi, KUDOH Suguru N., KAWANO Satoyuki

    The Proceedings of Mechanical Engineering Congress, Japan   2021 ( 0 )   J222-06   2021( eISSN:24242667

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    <p>In this research, the convection dynamics of surrounding fluid in optical trapping of tiny particles is investigated both experimentally and numerically. In the experimental part, a tightly focused laser with the wavelength of 1064 nm is irradiated to polystyrene beads with the diameter of 500 nm or 1 um dispersed in water.. The laser plays two roles: (i) it optically traps the particle at the focal spot and (ii) drives the overall particle motion toward the focal spot. The latter effect occurs away from the region where the laser is irradiated, and reaches several tens of micrometers; thus, this long-range particle transport is not induced by the direct action of the laser, but rather by the surrounding fluid motion. In the numerical part, we try to explain this long-range transport by using the simulation of a simple fluid model that includes optical effects both on the particles and the fluid. The model is based on the Navier-Stokes equations with the Boussinesq approximation and additionally includes two optical effects. One is a force acting on the fluid generated by the optically induced motion of suspended particles and the another is a local heat generated in the fluid by the photothermal effect due to the absorption of the laser. By comparing the experimental and numerical results, it is shown that the buoyancy effect by photothermal heating, which has been considered as the cause of the long-range particle transport, is overwhelmed by the effect of the optically induced particle motion.</p>

    DOI: 10.1299/jsmemecj.2021.j222-06

  • Student Presentation Award—Report on the Fourth Award Selection Process— Reviewed

    SUDO Yuki, KODERA Noriyuki, HOSOKAWA Chie

    The Biophysical Society of Japan General Incorporated Association, Seibutsu Butsuri   60 ( 1 )   47 - 48   2020( ISSN:0582-4052

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    Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    DOI: 10.2142/biophys.60.047

    CiNii Article

  • バイオミディア 生命現象を見る・照るためのフォトニクス技術 Reviewed

    細川 千絵

    (公社)日本生物工学会 生物工学会誌   96 ( 9 )   539 - 539   2018.09( ISSN:0919-3758

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    Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Kind of work:Single Work  

  • Response Property of Neuronal Networks Stimulated with a Focused Femtosecond Laser Reviewed

    細川 千絵, 藤岡 祐次, 工藤 卓, 田口 隆久

    レーザー学会研究委員会, レーザー学会研究会報告 = Reports the on topical meeting of the Laser Society of Japan   17 ( 25 )   41 - 45   2017.10

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    Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    CiNii Article

  • Laser-induced Perturbation Into Neuronal Networks Toward Manipulation of Cellular Functions Reviewed

    細川 千絵

    化學工業   68 ( 5 )   340 - 345   2017.05( ISSN:0451-2014

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    Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Kind of work:Single Work  

    CiNii Article

  • Local Manipulation of Neuronal Network with Focused Laser-Induced Perturbation Reviewed

    細川千絵

    レーザー学会, レーザー研究   44 ( 4 )   244 - 249   2016.04( ISSN:0387-0200

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    Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Kind of work:Single Work  

    CiNii Article

    J-GLOBAL

  • 集光レーザーを用いた神経細胞ネットワークの局所光操作 Reviewed

    HOSOKAWA Chie

    Molecular Electronics and Bioelectronics   26 ( 2 )   69 - 72   2015.05

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    Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Kind of work:Single Work  

  • 集光レーザービームの光摂動による神経細胞内分子動態の集合操作 Reviewed

    細川 千絵

    生物物理   54 ( 6 )   325 - 326   2014.11( ISSN:0582-4052

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    Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • ニューロサイエンスの最新情報 フェムト秒レーザー光による単一神経細胞刺激 Reviewed

    細川 千絵

    Clinical Neuroscience   32 ( 2 )   227 - 229   2014.02( ISSN:0289-0585

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    Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • 神経細胞表面分子の局所光操作に向けたプラズモン光ピンセット手法の開発 Reviewed

    宮内康平, 宮内康平, 田和圭子, 工藤卓, 田口隆久, 細川千絵

    電気学会電子・情報・システム部門大会講演論文集(CD-ROM)   2014   2014

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    J-GLOBAL

  • 共鳴レーザー照射による神経細胞接着分子の光捕捉 Reviewed

    武田尚子, 武田尚子, 工藤卓, 田口隆久, 田口隆久, 細川千絵, 細川千絵

    電気学会電子・情報・システム部門大会講演論文集(CD-ROM)   2013   2013

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    J-GLOBAL

  • Optical perturbation of intracellular molecular dynamics of single neuron in living neuronal network Reviewed

    HOSOKAWA Chie, TAKEDA Naoko, TAKEDA Naoko, UEDA Yusuke, UEDA Yusuke, KUDOH Suguru N., TAGUCHI Takahisa, TAGUCHI Takahisa

    生物物理   53 ( Supplement 1-2 (Web) )   2013( ISSN:0582-4052

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    J-GLOBAL

  • レーザ光による神経細胞の局所機能操作 : 細胞診断、細胞治療のためのレーザ技術の開発に向けて (特集 細胞レベルで健康を診る) Reviewed

    細川 千絵

    日本工業出版 光アライアンス   23 ( 10 )   22 - 26   2012.10( ISSN:0917-026X

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    CiNii Article

  • M-sequences from neural loop circuits Reviewed

    TAMURA Shinichi, NISHITANI Youshi, HOSOKAWA Chie, MIZUNO-MATSUMOTO Yuko, CHEN Yen-Wei, MIYOSHI Tomomitsu, SAWAI Hajime

    The Institute of Electronics, Information and Communication Engineers, IEICE technical report.   111 ( 389 )   121 - 126   2012.01( ISSN:0913-5685

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    In circuit theory it is well known that loop circuit with a feedback link generates a pseudo-random sequence including an M-sequence with maximum period length. On the other hand, it remains unclear how memory is stored in neural circuits of the human brain. We propose that M-sequence composes temporal independent basis functions in brain neural network for communication and memory storage. We have already shown its feasibility by computer simulations that show a loop circuit can be copied to another area by back-propagation leaning algorithm; this is a key function of brain intelligence, which includes functions such as memory, association, reasoning, and abstraction. We found M-sequence responses in the instantaneous firing rates from cultured neural networks of published data and our own data, which suggest the existence of loop circuits with a feedback link composed of 3 to 5 neuron cells. Analyzing the time sequence of spike intervals of cultured neural network with the "auto mutual-information function," we also find evidence that suggests the existence of loop circuits with a feedback link composed of 3, 4 neurons.

    CiNii Article

  • 細胞レベルで健康を診る レーザ光による神経細胞の局所機能操作=細胞診断,細胞治療のためのレーザ技術の開発に向けて=

    細川千絵

    光アライアンス   23 ( 10 )   22 - 26   2012( ISSN:0917-026X

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    CiNii Article

    J-GLOBAL

  • 1D1546 Assembling dynamics of optically trapped synaptic vesicles in neuronal cell(Development, Differentiation, Neuroscience,Oral Presentation,The 50th Annual Meeting of the Biophysical Society of Japan) Reviewed

    Ueda Yusuke, Kudoh Suguru N., Taguchi Takahisa, Hosokawa Chie

    The Biophysical Society of Japan General Incorporated Association, Seibutsu Butsuri   52   S26   2012

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  • Analyzing read-out pattern from brain neuronal network based on loop circuit hypothesis Reviewed

    NISHITANI Yoshi, HOSOKAWA Chie, TAMURA Shin-ichi

    一般社団法人電子情報通信学会, IEICE technical report   110 ( 461 )   101 - 106   2011.02( ISSN:09135685

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    We consider that the memory is composed of loop neural circuit in the cerebral cortex. We have already shown a feasibility of self organization of loop neural circuit for memory, association, and abstraction in our previous study. However, it remains unclear that how the memory is stored and transferred in real neural circuit. In this study, we found pseudo random sequences from cultured neuron networks, which suggest the existence of loop circuit with 3 elements and feedback link.

  • 細胞操作技術の最前線(最終回) 医療応用 バイオロボティクスへの発展 Reviewed

    工藤 卓, 細川 千絵

    (株)オーム社 Medical Bio   7 ( 3 )   36 - 39   2010.05( ISSN:1881-9354

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  • 細胞操作技術の最前線(最終回) 医療応用 バイオロボティクスへの発展

    工藤 卓, 細川 千絵

    Medical Bio   7 ( 3 )   36 - 39   2010( ISSN:1881-9354

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    CiNii Article

    J-GLOBAL

  • Preparation of glass beads incorporating quantum dots for use as fluorescent reagent and neuronal cell imaging Reviewed

    安藤昌儀, LI Chunliang, YANG Ping, 村瀬至生, 細川千絵, 田口隆久

    一般社団法人電子情報通信学会, 電子情報通信学会技術研究報告   110 ( 280(MI2010 66-80) )   57 - 62   2010( ISSN:0913-5685

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    Quantum dots (QDs) show bright photoluminescence whose emission wavelength can be controlled by their particle size and composition. Compared with organic fluorescent dyes, QDs are more resistant to light irradiation. Because of those advantages, QDs attract much interest as novel bright fluorescent reagents. Photoluminescent QDs are synthesized in solution. However, QDs in solution are not very stable and tend to degradate. Therefore, we developed techniques to incorporate multiple QDs such as CdSe and InP in a small glass bead (diameter: several tens nm) by using sol-gel method at room temperature and under atmospheric pressure. The QD-incorporating glass beads are water-dispersible and emit bright photoluminescence. When dispersed in buffer solution for biological use, the glass beads showed much higher stability and light-resistance than commercial polymer-coated QDs. It was possible to obtain high-resolution fluorescence image of living neuronal cells by introducing the glass beads in the cells by endocytosis.

  • 光圧による液中ナノ粒子の捕捉・集合ダイナミクス (特集 光で動かす) Reviewed

    細川 千絵, 吉川 裕之, 増原 宏

    日本工業出版 光アライアンス   18 ( 7 )   19 - 24   2007.07( ISSN:0917026X

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    CiNii Article

  • Optical Trapping, Assembly, and Surface Fixation of Nanoparticles in Liquid Reviewed

    YOSHIKAWA HIROYUKI, HOSOKAWA CHIE, ITO SHOJI, MASUHARA HIROSHI

    The Surface Science Society of Japan, 表面科学   26 ( 11 )   681 - 688   2005.11( ISSN:0388-5321|1881-4743

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    The intense electromagnetic field produced by a focused laser beam attracts microparticles, dispersed in liquid, into the focal spot. This is known as optical trapping and used to manipulate microparticles dispersed in liquid. If the size of the particles is much smaller than that of the focal spot, i.e. the order is of wavelength, a number of particles are trapped in the focal spot. This means that the focal spot of the laser beam acts as a microcage for nanoparticles and the confined nanoparticles form a microassembly. Nanoparticles are attractive materials for their characteristic chemical, physical, and optical properties. We have studied "optical assembly" of nanoparticles and found the following unique properties. (1) By a single particle counting measurement, it was revealed that the assembling process of polystyrene nanoparticles is dependent on laser power and concentration. (2) It was demonstrated that the assembly structure and optical properties of gold-nanoparticles can be controlled by optical force.

    DOI: 10.1380/jsssj.26.681

    CiNii Article

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Presentations

  • 集光フェムト秒光渦を用いた神経活動誘発 Domestic conference

    瀬川夕海、増井恭子、細川千絵

    第71回応用物理学会春季学術講演会  2024.03 

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    Presentation type:Oral presentation (general)  

  • 集光レーザービームの光圧による細胞膜表在分子モデルの拡散制御 Domestic conference

    森山俊哉, 谷本泰士, 増井恭子, 細川千絵

    第71回応用物理学会春季学術講演会  2024.03 

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    Presentation type:Oral presentation (general)  

  • Manipulation of Neuronal Network Dynamics with a Focused Femtosecond Laser International conference

    Y. Segawa, W. Minoshima, K. Masui, C. Hosokawa

    16th International Symposium on Nanomedicine (ISNM2023)  2023.11 

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    Presentation type:Poster presentation  

    Venue:Osaka Metropolitan Univ., Osaka  

  • Molecular Dynamics under Optical Trapping in Neuronal Cells Analyzed by Raman Spectroscopy International conference

    K. Nishimura, K. Masui, C. Hosokawa

    16th International Symposium on Nanomedicine (ISNM2023)  2023.11 

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    Presentation type:Poster presentation  

    Venue:Osaka Metropolitan Univ., Osaka  

  • Optical Manipulation of Molecules in the Model Biological Membrane International conference

    Y. Tanimoto, S. Moriyama, K. Masui, C. Hosokawa

    16th International Symposium on Nanomedicine (ISNM2023)  2023.11 

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    Presentation type:Poster presentation  

    Venue:Osaka Metropolitan Univ., Osaka  

  • Formation of ultralong liposome tubes by laser-induced microbubble Domestic conference

    A. Noguchi, K. Yuyama, C. Hosokawa, Y. Tanimoto, Y. Tsuboi

    第61回日本生物物理学会年会  2023.11  日本生物物理学会

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    Presentation type:Poster presentation  

  • Manipulation of lipid membrane domains in artificial membrane by optical tweezers Domestic conference

    Y. Tanimoto, S. Moriyama, K. Masui, C. Hosokawa

    第61回日本生物物理学会年会  2023.11  日本生物物理学会

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    Presentation type:Poster presentation  

  • 集光フェムト秒レーザーの高頻度照射により誘発された神経活動の時空間パターン(Spatio-temporal patterns of neuronal activity induced by high-frequency irradiation with a focused femtosecond laser) Domestic conference

    Otani Kan, Segawa Yumi, Minoshima Wataru, Masui Kyoko, Hosokawa Chie

    第61回日本生物物理学会年会   2023.11  (一社)日本生物物理学会

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    Presentation type:Poster presentation  

  • Neuronal circuit modulation at single-cell level using a focused femtosecond laser International conference

    Yumi Segawa, Kyoko Masui, Chie Hosokawa

    Society for Neuroscience Annual Meating (Neuroscience 2023)  2023.11  Society for Neuroscience

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    Presentation type:Poster presentation  

    Venue:Washington, D.C., USA  

  • 顕微ラマン分光計測による神経細胞内分子の光捕捉過程 Domestic conference

    西村和真, 増井恭子, 細川千絵

    2023 年度日本分光学会年次講演会  2023.10  日本分光学会

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    Presentation type:Poster presentation  

  • エンド-β-N-アセチルグルコサミニダーゼHSの立体構造とその機能発現 Domestic conference

    倉内 郁哉, 大倉 和貴, 森 真司, 米澤 健人, 清水 伸隆, 田中 里佳, 細川 千絵, 宮原 郁子, 伊藤 和央

    日本生化学会大会プログラム・講演要旨集  2023.10  (公社)日本生化学会

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    Presentation type:Oral presentation (general)  

  • レーザー摂動による神経活動ダイナミクスの制御 Invited Domestic conference

    細川千絵

    日本学術振興会R041バイオ・分子・ナノテクノロジー融合委員会 第4回研究会  2023.09  日本学術振興会R041バイオ・分子・ナノテクノロジー融合委員会

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    Presentation type:Oral presentation (invited, special)  

    Venue:静岡県浜松市  

  • レーザー誘起マイクロバブルによるリポソームの融合とウルトラロングチューブ形成 Domestic conference

    野口明美、柚山健一、細川千絵、谷本泰士、坪井泰之

    第84回応用物理学会秋季学術講演会  2023.09 

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    Presentation type:Oral presentation (general)  

  • 光ピンセットによるタンパク質結晶化 Domestic conference

    西室愛実, 谷本泰士, 増井恭子, 宮原郁子, 細川千絵

    第12回有機分子・バイオエレクトロニクスの未来を拓く若手研究者討論会  2023.09  有機分子・バイオエレクトロニクス分科会

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    Presentation type:Oral presentation (general)  

  • 顕微ラマン分光計測を用いた光圧下における神経細胞内分子動態変化 Domestic conference

    西村和真, 増井恭子, 細川千絵

    第84回応用物理学会秋季学術講演会  2023.09 

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    Presentation type:Oral presentation (general)  

  • 神経細胞内シナプス小胞群の光捕捉による神経活動時空間パターンの変調 Domestic conference

    箕嶋渉, 安田健人, 増井恭子, 細川千絵

    第84回応用物理学会秋季学術講演会  2023.09 

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    Presentation type:Oral presentation (general)  

  • 集光フェムト秒レーザーを用いた生体膜加工による脂質ラフトモデル構築 Domestic conference

    谷本泰士, 瀬川夕海, 増井恭子, 細川千絵

    第84回応用物理学会秋季学術講演会  2023.09 

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    Presentation type:Oral presentation (general)  

  • 集光フェムト秒光渦ビームを用いた光重合反応による微細構造形成 Domestic conference

    松本義久, 増井恭子, 細川千絵

    第84回応用物理学会秋季学術講演会  2023.09 

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    Presentation type:Oral presentation (general)  

  • 光圧下における支持脂質二分子膜上分子の動態 Domestic conference

    森山俊哉, 谷本泰士, 増井恭子, 細川千絵

    第84回応用物理学会秋季学術講演会  2023.09 

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    Presentation type:Oral presentation (general)  

  • 集光フェムト秒レーザーによる単一細胞レベルの神経活動操作 Domestic conference

    瀬川夕海, 増井恭子, 細川千絵

    第12回有機分子・バイオエレクトロニクスの未来を拓く若手研究者討論会  2023.09  有機分子・バイオエレクトロニクス分科会

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    Presentation type:Oral presentation (general)  

  • Single particle tracking analysis of neurotransmitter receptors with resonant optical tweezers Domestic conference

    T. Miyazaki, K. Ueno, Y. Tanimoto, K. Masui, C. Hosokawa

    2023年電気学会 電子・情報・システム部門大会  2023.08 

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    Presentation type:Oral presentation (general)  

  • 集光フェムト秒レーザーにより誘発された神経活動の電気生理学的計測 Domestic conference

    瀬川夕海, 増井恭子, 細川千絵

    第46回日本神経科学大会  2023.08  日本神経科学会

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    Presentation type:Poster presentation  

  • 初代培養海馬神経細胞の神経回路網形成に伴う還元型チトクロムc分布変化のラマンイメージング Domestic conference

    増井恭子, 長野貴裕, 谷知己, 名和靖矩, 藤田克昌, 石飛秀和, 藤田聡史, 細川千絵, 井上康志

    第46回日本神経科学大会  2023.08  日本神経科学会

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    Presentation type:Poster presentation  

  • 神経細胞内シナプス小胞群の光捕捉による神経電気活動制御 Domestic conference

    箕嶋渉, 安田健人, 増井恭子, 細川千絵

    2023年電気学会 電子・情報・システム部門大会  2023.08 

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    Presentation type:Oral presentation (general)  

  • 集光フェムト秒レーザー照射に伴う単一神経細胞の電気生理学的特徴 Domestic conference

    瀬川夕海, 増井恭子, 細川千絵

    2023年電気学会 電子・情報・システム部門大会  2023.08 

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    Presentation type:Oral presentation (general)  

  • エンド-β-N-アセチルグルコサミニダーゼHSの基質認識機構 Domestic conference

    倉内郁哉、大倉和貴、田中里佳、清水伸隆、米沢健人、細川千絵、伊藤和央、宮原郁子

    第23回グライコサイエンスフォーラム  2023.07 

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    Presentation type:Poster presentation  

    Venue:大阪公立大学、大阪府大阪市  

  • Optical trapping dynamics of molecules in cultured hippocampal neurons analyzed by Raman spectroscopy International conference

    K. Nishimura, K. Masui, C. Hosokawa

    The 31st International Conference on Photochemistry (ICP2023)  2023.07  ICP2023

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    Presentation type:Poster presentation  

    Venue:Sapporo, Japan  

  • Optical manipulation of cellular functions in neuronal networks using a focused laser beam International conference

    C. Hosokawa, T. Kishimoto, W. Minoshima, K. Masui

    The 31st International Conference on Photochemistry (ICP2023)  2023.07  ICP2023

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    Presentation type:Oral presentation (general)  

    Venue:Sapporo, Japan  

  • Regulation of molecular dynamics on substrate-supported lipid bilayer by optical tweezers International conference

    S. Moriyama, Y. Tanimoto, K. Masui, C. Hosokawa

    The 31st International Conference on Photochemistry (ICP2023)  2023.07  ICP2023

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    Presentation type:Poster presentation  

    Venue:Sapporo, Japan  

  • 共鳴レーザー波長に依存した神経伝達物質受容体分子の光捕捉過程 Invited Domestic conference

    2023.06  電気学会

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    Presentation type:Oral presentation (invited, special)  

  • 光ピンセットを用いた支持脂質二分子膜上の分子動態操作 Domestic conference

    2023.06  光化学協会

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    Presentation type:Poster presentation  

  • フェムト秒光渦レーザー照射による微細構造の作製 Domestic conference

    2023.06  光化学協会

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    Presentation type:Poster presentation  

  • エンド-β-N-アセチルグルコサミニダーゼHSの構造と機能発現 Domestic conference

    倉内郁哉、大倉和貴、森真司、米沢健人、清水伸隆、田中里佳、細川千絵、宮原郁子、伊藤和央

    第23回日本蛋白質科学会年会  2023.05  日本蛋白質科学会

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    Presentation type:Poster presentation  

  • Mechanisms of neuronal stimulation with a focused nanosecond optical vortex International conference

    Y. Segawa, Y. Tanimoto, K. Yuyama, W. Minoshima, K. Masui, T. Omatsu, C. Hosokawa

    The 10th Optical Manipulation and Structured Materials Conference (OMC2023)   2023.04  OMC2023

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    Presentation type:Oral presentation (general)  

    Venue:Yokohama, Japan  

  • Network-wide neuronal activity under optical trapping of synaptic vesicles in cultured neurons International conference

    W. Minoshima, T. Yasuda, K. Masui, C. Hosokawa

    The 10th Optical Manipulation and Structured Materials Conference (OMC2023)   2023.04  OMC2023

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    Presentation type:Oral presentation (general)  

    Venue:Yokohama, Japan  

  • 神経細胞内シナプス小胞群の光捕捉過程における神経活動パターン解析 Domestic conference

    2023.03  応用物理学会

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    Presentation type:Oral presentation (general)  

  • 集光フェムト秒レーザーを⽤いた⽣体膜加⼯による細胞由来⼈⼯⽣体膜の作製 Domestic conference

    谷本泰士, 増井恭子, 細川千絵

    第70 回応用物理学会春季学術講演会  2023.03 

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    Presentation type:Oral presentation (general)  

  • 光圧下における神経細胞内分⼦動態の顕微ラマン分光解析 Domestic conference

    西村和真, 増井恭子, 細川千絵

    第70 回応用物理学会春季学術講演会  2023.03 

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  • 集光フェムト秒レーザー照射に伴う神経回路網の誘発応答パターン Domestic conference

    2023.03  応用物理学会

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    Presentation type:Oral presentation (general)  

  • 集光フェムト秒レーザーの⾼頻度照射により誘発された神経活動 Domestic conference

    大谷 莞, 瀬川 夕海, 箕嶋 渉, 増井 恭子, 細川 千絵

    第70 回応用物理学会春季学術講演会  2023.03 

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  • X-ray crystallography of Endo-β-N-acetylglucosaminidase HS Domestic conference

    倉内郁哉, 大倉和貴, 田中里佳, 細川千絵, 伊藤和央, 宮原郁子

    2023年量子ビームサイエンスフェスタ  2023.03 

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    Presentation type:Poster presentation  

  • Three-dimensional structure and domains constructing endo-β-N-acetylglucosaminidase HS Domestic conference

    倉内郁哉, 大倉和貴, 森真司, 米澤健人, 清水伸隆, 田中里佳, 細川千絵, 宮原郁子, 伊藤和央

    日本農芸化学会大会講演要旨集(Web)  2023.03 

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  • 表面シラン化を経由した CdSe 系量子ドット分散 ガラスカプセルの合成と耐光性評価 Domestic conference

    村瀬至生, 細川千絵, 李春亮

    日本セラミックス協会 2023年年会  2023.03  日本セラミックス協会

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  • 共鳴効果を用いた神経細胞表面分子の光捕捉 Domestic conference

    宮崎達夢, 植野昂, 谷本泰士, 増井恭子, 細川千絵

    第1回学際化学若手育成シンポジウム  2023.03  大阪公立大学

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    Presentation type:Poster presentation  

    Venue:大阪公立大、大阪府大阪市  

  • 集光レーザービームの光摂動による神経活動ダイナミクスの制御 Invited Domestic conference

    Chie Hosokawa

    第10回ライフサイエンス談話会  2023.03  大阪公立大学

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    Venue:大阪大学、大阪府豊中市  

  • 細胞・生体分子の光イメージングと光操作 Invited Domestic conference

    Chie Hosokawa

    学術変革領域「キラル光物質科学」+自然科学研究機構新分野創成センターワークショップ  2023.03  自然科学研究機構新分野創成センター

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    Presentation type:Oral presentation (invited, special)  

    Venue:大阪大学、大阪府豊中市  

  • Laser-induced perturbation into neuronal networks International conference

    C. Hosokawa

    Structured Light for Life Workshop  2023.02 

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    Venue:Adelaide, Australia  

  • Neuronal activity modification by focused femtosecond laser irradiation International conference

    Y. Segawa, K. Masui, C. Hosokawa

    Structured Light for Life Workshop  2023.02 

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    Venue:Adelaide, Australia  

  • 共鳴レーザー波長に依存した神経伝達物質受容体分子の光捕捉過程 Domestic conference

    宮崎達夢, 植野昂, 谷本泰士, 増井恭子, 細川千絵

    2022年 光・量子デバイス研究会「量子ビームによるナノ構造・界面形成とバイオメディカル応用」  2022.12  電気学会 電子・情報・システム部門(C部門)

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    Presentation type:Oral presentation (general)  

    Venue:兵庫県神戸市  

  • Laser-induced Perturbation into Neurons toward Manipulation of Neural Networks Invited International conference

    C. Hosokawa

    15th International Symposium on Nanomedicine (ISNM2022)  2022.12  ISNM2022

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    Presentation type:Oral presentation (invited, special)  

    Venue:Tokushima, Japan  

  • Simultaneous Measurement of Extracellular Potentials with Optical Trapping of Synaptic Vesicles in Neurons International conference

    T. Yasuda, W. Minoshima, K. Masui, C. Hosokawa

    15th International Symposium on Nanomedicine (ISNM2022)  2022.12  ISNM2022

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    Presentation type:Poster presentation  

    Venue:Tokushima, Japan  

  • Optical Resonance Trapping of Nanoparticles by Two-Color Laser Beams International conference

    K. Ueno, T. Miyazaki, Y. Tanimoto, K. Masui, C. Hosokawa

    15th International Symposium on Nanomedicine (ISNM2022)  2022.12 

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    Presentation type:Poster presentation  

    Venue:Tokushima Univ., Tokushima  

  • 集光レーザービームの光摂動による細胞内分子操作 Invited Domestic conference

    Chie Hosokawa

    第45回日本分子生物学会年会  2022.12 

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  • エンド -β-N-アセチルグルコサミニダーゼHS におけるドメインIV の役割 Domestic conference

    倉内郁哉, 大倉和貴, 米澤健人, 清水伸隆, 田中里佳, 細川千絵, 宮原郁子, 伊藤和央

    令和4 年(2022 年)度 日本結晶学会年会  2022.11 

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  • 集光フェムト秒レーザーによる単一神経細胞の活動変化 Domestic conference

    瀬川夕海, 増井恭子, 細川千絵

    第47回レーザー顕微鏡研究会&シンポジウム  2022.11  レーザー顕微鏡研究会

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    Venue:大阪大学、大阪府吹田市  

  • エンド-β-N-アセチルグルコサミニダーゼHS の立体構造とそのドメイン Domestic conference

    倉内郁哉, 大倉和貴, 米澤健人, 清水伸隆, 田中里佳, 細川千絵, 宮原郁子, 伊藤和央

    第95 回日本生化学会大会  2022.11 

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  • 単一蛍光性ナノ粒子の光捕捉における共鳴光波長依存性 Domestic conference

    植野昂, 増井恭子, 細川千絵

    応用物理学会関西支部2022年度75周年記念講演会  2022.11  応用物理学会関西支部

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    Venue:大阪大学、大阪府吹田市  

  • 集光フェムト秒レーザーの短時間照射により誘発された神経活動(Neuronal activities induced by short-time irradiation of a focused femtosecond laser) Domestic conference

    大谷莞, 瀬川夕海, 箕嶋渉, 増井恭子, 細川千絵

    第60回日本生物物理学会年会  2022.09  (一社)日本生物物理学会

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    Venue:函館アリーナ、北海道函館市  

  • 神経細胞内シナプス小胞群の光捕捉下における神経活動測定(Neuronal activity measurement under optical trapping of synaptic vesicles in neurons) Domestic conference

    安田健人, 箕嶋渉, 増井恭子, 細川千絵

    第60回日本生物物理学会年会  2022.09  (一社)日本生物物理学会

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    Venue:函館アリーナ、北海道函館市  

  • 集光フェムト秒レーザーに誘発された神経活動のパッチクランプ計測 Domestic conference

    2022.09  応用物理学会

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  • 結合エントロピーによる神経細胞間の結合強度解析 Domestic conference

    2022.09  応用物理学会

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  • レーザー摂動による神経細胞ネットワークの操作 Domestic conference

    2022.09  応用物理学会

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  • 光捕捉された単一色素含有ナノ粒子の共鳴光波長依存性 Domestic conference

    植野昂, 増井恭子, 細川千絵

    第83 回応用物理学会秋季学術講演会  2022.09 

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  • Drastic photostability improvement proved by single particle detection method for emitting CdSe quantum dots after the judicious surface silanization International conference

    N. Murase, C. Hosokawa, C. Li

    Hole Burning, Single Molecule, and Related Spectroscopies 2022 (HBSM2022)  2022.09  HBSM2022

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    Venue:Bayreuth, Germany  

  • 集光レーザービームの光摂動を用いた細胞機能操作 Invited Domestic conference

    Chie Hosokawa

    第11回 有機分子・バイオエレクトロニクスの未来を拓く若手研究者討論会  2022.09 

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  • レーザー摂動による神経細胞ネットワークの操作 Domestic conference

    Chie Hosokawa

    2022.09 

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  • 共鳴ラマンイメージングを用いた培養神経細胞ネットワーク形成に伴う分子分布変化 Domestic conference

    増井恭子, 長野貴裕, 名和靖矩, 谷知己, 藤田克昌, 石飛秀和, 藤田聡史, 細川千絵, 井上康志

    2022.08 

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  • 神経回路網の発達に依存して変化する結合強度の移動エントロ ピー解析 Domestic conference

    箕嶋渉, 瀬川夕海, 細川千絵

    2022 年電気学会 電子・情報・システム部門大会  2022.08 

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  • Spatiotemporal dynamics of evoked neuronal responses induced by a focused femtosecond laser Domestic conference

    Y. Segawa, W. Minoshima, K. Masui, C. Hosokawa

    2022 年電気学会 電子・情報・システム部門大会  2022.08 

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  • Drastic photostability improvement proved by single particle detection method for emitting CdSe quantum dots after the judicious surface silanization International conference

    N. Murase, C. Hosokawa, C. Li

    Hole Burning, Single Molecule, and Related Spectroscopies 2022 (HBSM2022)  2022.08 

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  • Raman imaging of primary cultured hippocampal neuron for evaluating neuronal maturation International conference

    T. Nagano, K. Masui, Y. Nawa, H. Ishitobi, T. Tani, S. Fujita, K. Fujita, C. Hosokawa, Y. Inouye

    The 15th Pacific Rim Conference on Lasers and Electro-Optics (CLEO-PR2022)  2022.08  CLEO-PR2022

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    Venue:Sapporo, Japan  

  • Detection of Liposomes Encapsulating Neurotransmitters by Optical Trapping Raman Spectroscopy International conference

    K. Masui, Y. Nawa, S. Tokumitsu, M. Kawarai, W. Minoshima, T. Tani, S. Fujita, H. Ishitobi, C. Hosokawa, Y. Inouye

    The 15th Pacific Rim Conference on Lasers and Electro-Optics (CLEO-PR2022)  2022.08 

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    Venue:Sapporo, Hokkaido  

  • Single-Neuron Stimulation with a Focused Femtosecond Laser International conference

    Y. Segawa, W. Minoshima, K. Masui, C. Hosokawa

    The 15th Pacific Rim Conference on Lasers and Electro-Optics (CLEO-PR2022)  2022.08  CLEO-PR2022

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    Venue:Sapporo, Japan  

  • 集光フェムト秒レーザーの短時間照射により誘発された神経活動(Neuronal activities induced by short-time irradiation of a focused femtosecond laser) Domestic conference

    Otani Kan, Segawa Yumi, Minoshima Wataru, Masui Kyoko, Hosokawa Chie

    生物物理  2022.08  (一社)日本生物物理学会

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  • 神経細胞内シナプス小胞群の光捕捉下における神経活動測定(Neuronal activity measurement under optical trapping of synaptic vesicles in neurons) Domestic conference

    Yasuda Taketo, Minoshima Wataru, Masui Kyoko, Hosokawa Chie

    生物物理  2022.08  (一社)日本生物物理学会

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  • レーザー摂動による細胞機能操作 Invited Domestic conference

    細川千絵

    2022年度有機分子・バイオエレクトロニクス分科会6⽉研究会「有機分⼦・バイオエレクトロニクス研究の最前線」  2022.06  有機分子・バイオエレクトロニクス分科会

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    Venue:東京都江東区  

  • エンド-β-N-アセチルグルコサミニダーゼHSの立体構造と機能 Domestic conference

    倉内郁哉, 大倉和貴, 田中里佳, 細川千絵, 伊藤和央, 宮原郁子, 米澤健人, 清水伸隆

    2022年日本蛋白質科学会年会  2022.06 

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  • エンド-β-N-アセチルグルコサミニダーゼHSの機能とドメイン構造 Domestic conference

    倉内郁哉, 大倉和貴, 森真司, 米澤健人, 清水伸隆, 田中里佳, 細川千絵, 宮原郁子, 伊藤和央

    第22回関西グライコサイエンスフォーラム  2022.05 

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  • Optical trapping dynamics of nanoparticles by resonant optical responses International conference

    K. Ueno, T. Kishimoto, K. Masui, C. Hosokawa

    The 8th Optical Manipulation and Structured Materials Conference (OMC2022)  2022.04  OMC2022

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    Venue:Yokohama, Japan  

  • Focused Femtosecond Laser-induced Single Neuronal Electrical Activity Domestic conference

    瀬川夕海, 箕嶋渉, 増井恭子, 細川千絵

    応用物理学会春季学術講演会講演予稿集(CD-ROM)  2022.03 

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  • Optical Trapping and Assembling Process of Synaptic Vesicles in Neurons Cultured on Multi-Electrode Arrays Domestic conference

    安田健人, 箕嶋渉, 増井恭子, 細川千絵

    応用物理学会春季学術講演会講演予稿集(CD-ROM)  2022.03 

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  • Diffusion Properties of Lipid Bilayers in an Optical Trap Domestic conference

    石原悠人, 増井恭子, 箕嶋渉, 杭田芙子, 森垣憲一, 細川千絵

    応用物理学会春季学術講演会講演予稿集(CD-ROM)  2022.03 

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  • Optical trapping of cell surface molecules of neurons on a Bull’s eye-type plasmonic chip Domestic conference

    小泉喬史, 永末智也, 田和圭子, 細川千絵

    応用物理学会春季学術講演会講演予稿集(CD-ROM)  2022.03 

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  • X-ray crystallography of Endo-β-N-acetylglucosaminidase HS Domestic conference

    倉内郁哉, 大倉和貴, 田中里佳, 細川千絵, 伊藤和央, 宮原郁子

    量子ビームサイエンスフェスタ(Web)  2022.03 

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  • Optical Vortex-Induced Modulation of Neuronal Activity in Single Neurons Domestic conference

    箕嶋渉, 瀬川夕海, 増井恭子, 柚山健一, 尾松孝茂, 細川千絵

    応用物理学会春季学術講演会講演予稿集(CD-ROM)  2022.03 

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  • Live-cell Raman imaging of cultured hippocampal neurons in neuronal maturation Domestic conference

    長野貴裕, 増井恭子, 名和靖矩, 細川千絵, 石飛秀和, 谷知己, 藤田聡史, 井上康志

    応用物理学会春季学術講演会講演予稿集(CD-ROM)  2022.03 

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  • Single-cell stimulation in neuronal networks with a focused femtosecond laser International conference

    Y. Segawa, W. Minoshima, C. Hosokawa

    The International Chemical Congress of Pacific Basin Societies 2021 (Pacifichem2021)  2021.12 

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  • Deuterated glutamate-mediated electrical activity of neuronal networks measured by multi-electrode arrays International conference

    W. Minoshima, K. Masui, T. Tani, Y. Nawa, S. Fujita, H. Ishitobi, C. Hosokawa, Y. Inouye

    The International Chemical Congress of Pacific Basin Societies 2021 (Pacifichem2021)  2021.12 

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  • Optical trapping of cellular receptors on neurons toward manipulation of cellular activity Invited Domestic conference

    Chie Hosokawa

    The International Chemical Congress of Pacific Basin Societies 2021 (Pacifichem2021)  2021.12 

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  • Optical trapping of glutamate receptors on neuronal cells with a bull’s eye-type plasmonic chip Domestic conference

    T. Koizumi, T. Nagasue, K. Tawa, C. Hosokawa

    第58 回日本生物物理学会年会  2021.11 

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  • Spatio-temporal properties of neuronal activities induced by a focused femtosecond laser International conference

    Y. Segawa, W. Minoshima, C. Hosokawa

    The fourth International Workshop on Symbiosis of Biology and Nanodevices (IWSBN2021)  2021.11 

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  • Optical trapping dynamics of lipid molecules in artificial bilayers International conference

    Y. Ishihara, F. Kueda, K. Morigaki, C. Hosokawa

    The fourth International Workshop on Symbiosis of Biology and Nanodevices (IWSBN2021)  2021.11 

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  • Connection strengths in cultured neurons on multi-electrodes arrays evaluated by transfer entropy International conference

    W. Minoshima, Y. Segawa, C. Hosokawa

    The fourth International Workshop on Symbiosis of Biology and Nanodevices (IWSBN2021)  2021.11 

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  • Optical Trapping Dynamics of Cell Surface Molecules on Neurons Invited Domestic conference

    Chie Hosokawa

    The 14th International Symposium on Nanomedicine (ISNM2021)  2021.11 

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  • Endo-β-N-acetylglucosaminidase HSのX線結晶構造解析 Domestic conference

    令和3 年(2021 年度)結晶学会年会  2021.11 

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  • Bull's eye型プラズモニックチップによる神経細胞表面グルタミン酸受容体分子の光捕捉(Optical trapping of glutamate receptors on neuronal cells with a bull's eye-type plasmonic chip) Domestic conference

    Koizumi Takashi, Nagasue Tomoya, Tawa Keiko, Hosokawa Chie

    生物物理  2021.10  (一社)日本生物物理学会

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  • Evaluation of connection properties in neuronal networks by using transfer entropy Domestic conference

    箕嶋渉, 瀬川夕海, 細川千絵

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)  2021.09 

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  • Numerical simulation of assembling process of nanoparticles in an optical trap Domestic conference

    石原悠人, 箕嶋渉, 細川千絵

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)  2021.09 

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  • Detection of neurotransmitters encapsulated in artificial liposomes by optical-trapping Raman microscopy Domestic conference

    増井恭子, 増井恭子, 徳満俊介, 名和靖矩, 名和靖矩, 細川千絵, 細川千絵, 石飛秀和, 石飛秀和, 藤田聡史, 井上康志, 井上康志

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)  2021.09 

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  • Optical trapping dynamics of nanoparticles depending on the wavelength of resonance laser Domestic conference

    植野昂, 岸本龍典, 岸本龍典, 箕嶋渉, 細川千絵

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)  2021.09 

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  • Analysis of a fluid flow in optical trapping of tiny particle Domestic conference

    辻徹郎, 細川千絵, 細川千絵, 岸本龍典, 岸本龍典, 大久保匠, 工藤卓, 川野聡恭

    日本機械学会年次大会講演論文集(CD-ROM)  2021.09 

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  • レーザー摂動による細胞内分子操作と細胞機能制御への応用 Invited Domestic conference

    Chie Hosokawa

    学振174委員会 第71回研究会  2021.08 

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  • Acute effects of deuterated glutamate on neuronal activity in cultured hippocampal neurons Domestic conference

    W. Minoshima, K. Masui, T. Tani, Y. Nawa, S. Fujita, H. Ishitobi, C. Hosokawa, Y. Inouye

    第44 回日本神経科学大会  2021.07 

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  • Responses of neuronal networks activities evoked by a focused femtosecond laser Domestic conference

    Y. Segawa, W. Minoshima, C. Hosokawa

    第44 回日本神経科学大会  2021.07 

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  • プラズモニックチップによる神経細胞シナプス関連分子の可視化と操作 Invited Domestic conference

    Chie Hosokawa

    第20回プラズモニック化学シンポジウム  2021.06 

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  • Diffusion Properties of Artificial Lipid Bilayers Biased by Optical Forces Domestic conference

    石原悠人, 岸本龍典, 箕嶋渉, 杭田芙子, 森垣憲一, 細川千絵

    ナノ学会大会講演予稿集  2021.05 

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  • Optical trapping of nanoparticles suspended in water with a bull’s eye-type plasmonic chip International conference

    T. Koizumi, T. Nagasue, K. Tawa, C. Hosokawa

    The 7th Optical Manipulation and Structured Materials Conference (OMC2021)  2021.04 

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  • Fluid convection driven by suspended particles in optical trapping International conference

    T. Tsuji, C. Hosokawa, T. Kishimoto, T. Okubo, S. N. Kudoh, S. Kawano

    The 7th Optical Manipulation and Structured Materials Conference (OMC2021)  2021.04 

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  • 集光フェムト秒レーザー照射に伴う神経回路網の誘発応答解析 Domestic conference

    第68回応用物理学会春季学術講演会  2021.03 

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  • Bull’s eye型プラズモニックチップによるナノ粒子の光捕捉メカニズム Domestic conference

    第68回応用物理学会春季学術講演会  2021.03 

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  • 神経伝達物質受容体分子の光捕捉によるシナプス伝達効率の上昇 Domestic conference

    第68回応用物理学会春季学術講演会  2021.03 

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  • Molecular manipulation and activity control of neurons by optical trapping with a focused laser beam Invited Domestic conference

    2021.01 

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  • 集光フェムト秒レーザー照射に伴う神経回路網の活動変化 Domestic conference

    2020.12 

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  • Optical manipulation of cell surface molecules for direct control of neuronal activity Domestic conference

    2020.09 

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  • Lateral diffusion in lipid bilayers biased by optical forces Domestic conference

    2020.09 

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  • 神経伝達物質受容体の光捕捉にともなう神経活動頻度の増加 Domestic conference

    第81回応用物理学会秋季学術講演会  2020.09 

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  • Bull’s eye型プラズモニックチップを用いたナノ粒子の光捕捉過程 Domestic conference

    第81回応用物理学会秋季学術講演会  2020.09 

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  • 多電極アレイ上の神経回路網における重水素化グルタミン酸に活性化された神経活動特性 Domestic conference

    第81回応用物理学会秋季学術講演会  2020.09 

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  • 微小粒子の光捕捉過程における周囲流体の大規模流れ Domestic conference

    第81回応用物理学会秋季学術講演会  2020.09 

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  • 光圧による神経細胞シナプス機能分子の操作と細胞機能制御への応用 Domestic conference

    細川千絵

    分析化学討論会講演要旨集(Web)  2020.05 

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  • Neuronal electrical activity induced by optical trapping of neurotransmitter receptors on neuron International conference

    T. Kishimoto, S. N. Kudoh, T. Taguchi, C. Hosokawa

    2020.04 

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  • 重水素置換グルタミン酸濃度に依存した神経自発活動解析 Domestic conference

    第67回応用物理学会春季学術講演会  2020.03 

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  • 神経細胞グルタミン酸受容体分子の光捕捉過程における細胞膜電位変化 Domestic conference

    第67回応用物理学会春季学術講演会  2020.03 

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  • 光ピンセットを用いた神経細胞表面の分子操作過程の蛍光解析 Domestic conference

    2020.01 

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  • Fluorescence analysis of optical trapping and manipulation dynamics of cell surface molecules Domestic conference

    2020.01 

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  • Neuronal electrical activity induced by optical trapping of neurotransmitter receptors on neuron Domestic conference

    Tatsunori Kishimoto, Suguru N. Kudoh, Takahisa Taguchi, Chie Hosokawa

    Proceedings of SPIE - The International Society for Optical Engineering  2020 

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    © 2020 SPIE. AMPA-type glutamate receptor (AMPAR) is one of neurotransmitter receptors at excitatory synapses in neuronal cell. For realizing the artificial control of synaptic transmission, we have applied optical trapping of quantum-dot (QD) conjugated AMPARs on neuronal cells. Here, we demonstrate simultaneous measurement combined with optical trapping and patch-clamp recordings to evaluate the neuronal electrical activity. The relationship between optical trapping dynamics of QD-AMPARs located on neuronal cells and the neuronal electrical activity was discussed.

  • Simultaneous Measurements of Molecular Dynamics of Optically Trapped Glutamate Receptors and Electrical Activity in Neurons Invited International conference

    C. Hosokawa, T. Kishimoto, S. N. Kudoh, T. Taguchi

    2019.12 

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  • Live-cell Raman imaging of hippocampal neuronal cells International conference

    K. Masui, S. Morishita, T. Hamamoto, W. Minoshima, C. Hosokawa, V. R Daria, H. Ishitobi, Y. Inouye

    2019.11 

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  • Relationship between molecular dynamics of glutamate receptors in an optical trap and electrical activity in neurons International conference

    T. Kishimoto, S. N. Kudoh, T. Taguchi, C. Hosokawa

    International Workshop on Quantum Sensing & Biophotonics 2019 (IWQSB2019)  2019.11 

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    Venue:Osaka  

  • Relationship between optical trapping dynamics of neurotransmitter receptor and neuronal electrical activity International conference

    T. Kishimoto, S. N. Kudoh, T. Taguchi, C. Hosokawa

    2019.11 

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    Presentation type:Poster presentation  

    Venue:Ikuta Shrine Hall, Kobe, Japan  

  • Optical trapping of nanoparticle suspensions on a plasmonic chip and its application to manipulation of cell surface molecules Invited International conference

    Chie Hosokawa, Yuki Matsubayashi, Suguru N. Kudoh, Keiko Tawa

    2019.11 

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  • Molecular dynamics in an optical trap on living neuronal networks Invited International conference

    C. Hosokawa

    2019.11 

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  • 光圧による細胞表面分子操作における細胞電気活動計測 Domestic conference

    岸本龍典, 工藤卓, 田口隆久, 細川千絵

    OCU先端光科学シンポジウム  2019.10 

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    Venue:大阪市立大学、大阪府大阪市  

  • Relationship between molecular dynamics of glutamate receptors in an optical trap and electrical activity in neurons International conference

    T. Kishimoto, S. N. Kudoh, T. Taguchi, C. Hosokawa

    2019.09 

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  • 光圧による神経細胞ネットワークの機能分子操作 Invited Domestic conference

    細川千絵

    2019年光化学討論会   2019.09 

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    Venue:名古屋大学、愛知県名古屋市  

  • プラズモニックチップ上に吸着した量子ドット のCWレーザー励起 2光子発光イメージング Domestic conference

    大村祐貴, 松林佑基, 細川千絵, 田和圭子

    2019年光化学討論会   2019.09 

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    Venue:名古屋大学、愛知県名古屋市  

  • 光ピンセットを用いたAMPA型グルタミン酸受容体分子操作における神経電気活動変化(Neuronal electrical activity induced by optical trapping of AMPA-type glutamate receptors on neurons) Domestic conference

    Kishimoto Tatsunori, Kudoh Suguru, Taguchi Takahisa, Hosokawa Chie

    生物物理  2019.08  (一社)日本生物物理学会

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  • Laser-induced perturbation into cell-surface molecules on neurons Invited International conference

    Chie Hosokawa

    The 6th Awaji International Workshop on “Electron Spin Science & Technology: Biological and Materials Science Oriented Applications” (AWEST2019)  2019.06 

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    Venue:Awaji Yumebutai International Conference Center, Awaji Island, Hyogo  

  • Resonance laser effect on optical trapping of cell surface molecules International conference

    T. Kishimoto, S. N. Kudoh, T. Taguchi, C. Hosokawa

    2019.04 

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  • 溶液中ナノ粒子の光捕捉過程における共鳴レーザー照射効果 Domestic conference

    岸本龍典, 工藤卓, 田口隆久, 細川千絵

    応用物理学会春季学術講演会講演予稿集(CD-ROM)  2019.03 

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  • ナノ流体デバイスにおける光圧によるナノ粒子の操作と微小反応場の構築 Domestic conference

    西岡賢史, 岸本龍典, 辻川健寛, 細川千絵, 工藤卓, 許岩

    分析化学討論会講演要旨集  2019 

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  • ナノ流体デバイスと光圧の融合による単一ナノ粒子の捕捉・輸送・配置 Domestic conference

    西岡賢史, 岸本龍典, 辻川健寛, 細川千絵, 工藤卓, 許岩

    日本分析化学会年会講演要旨集(Web)  2019 

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  • Femtosecond Laser-induced Stimulation into Neuronal Networks Invited International conference

    C. Hosokawa, Y. Fujioka, S. N. Kudoh, T. Taguchi

    The 12th International Symposium on Nanomedicine (ISNM2018)  2018.12 

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  • 細胞表面分子操作のための共鳴効果を用いた光捕捉の検討 Domestic conference

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)  2018.09 

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  • プラズモニックディッシュ上のDi‐4‐ANEPPSの増強蛍光を用いた神経自発活動のリアルタイム計測 Domestic conference

    箕嶋渉, 細川千絵, 工藤卓, 田和圭子, 田和圭子

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)  2018.09 

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  • 海馬神経細胞のラマン分光計測 Domestic conference

    望月葵, 増井恭子, 増井恭子, 名和靖矩, 名和靖矩, 石飛秀和, 石飛秀和, 細川千絵, 細川千絵, DARIA Vincent, 藤田克昌, 藤田克昌, 井上康志

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)  2018.09 

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  • プラズモニックディッシュ上のDi‐4‐ANEPPSの増強蛍光を用いた神経自発活動のリアルタイム計測 Domestic conference

    箕嶋渉, 細川千絵, 工藤卓, 田和圭子

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)  2018.09 

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  • プラズモニックチップを用いた半導体ナノ粒子の光捕捉 Domestic conference

    松林佑基, 松林佑基, 田和圭子, 田和圭子, 工藤卓, 細川千絵, 細川千絵, 細川千絵

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)  2018.09 

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  • 集光フェムト秒レーザー刺激による神経活動の時空間ダイナミクス(Spatio-Temporal Dynamics of Neuronal Spikes Induced by a Focused Femtosecond Laser) Domestic conference

    Fujioka Yuji, Kudoh Suguru N., Taguchi Takahisa, Hosokawa Chie

    生物物理  2018.08  (一社)日本生物物理学会

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  • プラズモニックチップ上の増強蛍光による培養神経細胞の自発活動計測(Spontaneous activity in cultured neurons measured with the enhanced fluorescence on the plasmonic chip) Domestic conference

    Minoshima Wataru, Hosokawa Chie, Kudoh Suguru, Tawa Keiko

    生物物理  2018.08  (一社)日本生物物理学会

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  • ナノ流体デバイスと光圧の融合による単一ナノ粒子の捕捉・輸送と精密配置 Domestic conference

    西岡賢史, 岸本龍典, 岸本龍典, 細川千絵, 川端利幸, 辻川健寛, 野村俊之, 工藤卓, 許岩

    化学とマイクロ・ナノシステム学会研究会講演要旨集  2018.05 

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  • Optical trapping of quantum-dot conjugated AMPA-type receptors depened on initial assembling states Domestic conference

    Kishimoto Tatsunori, Maezawa Yasuyo, Kudoh Suguru N., Taguchi Takahisa, Hosokawa Chie

    OPTICAL MANIPULATION CONFERENCE  2018.04 

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  • 光マニピュレーションを用いた細胞操作 Invited Domestic conference

    細川千絵

    第111回テクノラボツアー「ナノ光マニピュレーションが拓く世界」  2018.03 

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    Venue:大阪府立大学  

  • 海馬神経細胞のラマンイメージング Domestic conference

    応用物理学会春季学術講演会講演予稿集(CD-ROM)  2018.03 

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  • 海馬神経細胞のラマンイメージング Domestic conference

    応用物理学会春季学術講演会講演予稿集(CD-ROM)  2018.03 

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  • 光ピンセットを用いた神経細胞分子操作における細胞内電気活動計測 Domestic conference

    応用物理学会春季学術講演会講演予稿集(CD-ROM)  2018.03 

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  • フェムト秒レーザー照射による神経細胞の光刺激過程 Domestic conference

    藤岡祐次, 藤岡祐次, 工藤卓, 田口隆久, 細川千絵, 細川千絵, 細川千絵

    応用物理学会春季学術講演会講演予稿集(CD-ROM)  2018.03 

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  • プラズモニックチップ上で培養した神経細胞表面AMPA受容体の光捕捉 Domestic conference

    応用物理学会春季学術講演会講演予稿集(CD-ROM)  2018.03 

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  • プラズモニックチップ上における神経自発活動の高感度蛍光イメージング Domestic conference

    箕嶋渉, 泉章太, 細川千絵, 工藤卓, 田和圭子

    応用物理学会春季学術講演会講演予稿集(CD-ROM)  2018.03 

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  • Fluorescent imaging of live hippocampal neurons with platinum nanoclusters Domestic conference

    Minami Fujieda, Kyoko Masui, Chie Hosokawa, Hidekazu Ishitobi, Yasushi Inouye

    Optics InfoBase Conference Papers  2018.01 

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    © 2018 OSA - The Optical Society. All rights reserved. Fluorescent imaging is a powerful technique to reveal how target cells or biomolecules work in biological tissues and cells. So far, organic dyes, fluorescent proteins, and quantum dots have been developed and widely used as a fluorescent probe. However, these fluorescent probes do not work well in certain conditions: they have cytotoxicity and steric hindrance due to inclusion of harmful atoms and/or their sizes. Hence, metal nanoclusters, that are composed of several metal atoms and emit fluorescence owing to quantum size effect, have been anticipated as a newly categorized fluorescent probe. Since the fluorescent wavelength depends on their sizes, it is possible to control the fluorescent wavelength by adjusting the number of composed atoms [1].

  • Optical trapping of quantum-dot-conjugated glutamate receptors on living neuronal cells Invited International conference

    C. Hosokawa, T. Kishimoto, Y. Maezawa, S. N. Kudoh, T. Taguchi

    The International Symposium on Nanomedicine 2017 (ISNM2017)  2017.12 

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    Venue:Tohoku University  

  • 集光レーザービームの光摂動による神経細胞ネットワークの操作 Invited Domestic conference

    細川千絵

    大阪大学生命機能研究科第175回研究交流会  2017.11  大阪大学生命機能研究科

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    Venue:大阪大学  

  • Response Property of Neuronal Networks Stimulated with a Focused Femtosecond Laser Domestic conference

    細川千絵, 藤岡祐次, 工藤卓, 田口隆久

    レーザー学会研究会報告  2017.10 

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  • 培養日数に依存して変化するAMPA受容体分子の光捕捉過程 Domestic conference

    岸本龍典, 前澤安代, 工藤卓, 田口隆久, 細川千絵

    電気学会電子・情報・システム部門大会講演論文集(CD-ROM)  2017.09 

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  • プラズモニックチップを用いた神経細胞表面に局在するAMPA受容体分子の光捕捉 Domestic conference

    松林佑基, 泉章太, 田和圭子, 工藤卓, 細川千絵

    電気学会電子・情報・システム部門大会講演論文集(CD-ROM)  2017.09 

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  • フェムト秒レーザー光刺激手法による神経回路網の誘発応答 Domestic conference

    藤岡祐次, 工藤卓, 田口隆久, 細川千絵

    電気学会電子・情報・システム部門大会講演論文集(CD-ROM)  2017.09 

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  • Bull’s Eyeプラズモニックチップによる培養神経回路網の膜電位蛍光イメージング Domestic conference

    箕嶋渉, 泉章太, 細川千絵, 工藤卓, 田和圭子

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)  2017.08 

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  • 集光フェムト秒レーザーによる単一神経細胞の光刺激メカニズム(Stimulation Mechanisms in Living Neuronal Cells with a Focused Femtosecond Laser) Domestic conference

    Fujioka Yuji, Kudoh Suguru N., Taguchi Takahisa, Hosokawa Chie

    生物物理  2017.08  (一社)日本生物物理学会

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  • 集光レーザービームの光摂動を利用した細胞機能操作手法の開発 Invited Domestic conference

    細川千絵

    第20回光科学異分野横断セミナー  2017.05 

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    Venue:大阪府立大学  

  • 集光レーザー摂動を利用した神経回路の機能操作 Invited Domestic conference

    細川千絵

    大阪大学フォトニクスセンター第52回フォトニクス・コロキアム  2017.04 

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  • Molecular dynamics in an optical trap of glutamate receptors labeled with quantum-dots on living neurons Domestic conference

    Kishimoto Tatsunori, Maezawa Yasuyo, Kudoh Suguru N., Taguchi Takahisa, Hosokawa Chie

    OPTICAL MANIPULATION CONFERENCE  2017.04 

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  • 光ピンセット集光時におけるAMPA受容体分子の動態観測 Domestic conference

    岸本龍典, 前澤安代, 細川千絵, 田口隆久, 工藤卓

    応用物理学会春季学術講演会講演予稿集(CD-ROM)  2017.03 

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  • フェムト秒レーザー光刺激に伴う誘発応答の細胞外電位計測とCa2+イメージングの同時計測 Domestic conference

    藤岡祐次, 中川裕太, 工藤卓, 田口隆久, 細川千絵

    応用物理学会春季学術講演会講演予稿集(CD-ROM)  2017.03 

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  • Response Property of Neuronal Networks Stimulated with a Focused Femtosecond Laser Domestic conference

    細川千絵, 藤岡祐次, 工藤卓, 田口隆久

    レーザー学会研究会報告  2017 

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  • Optical Trapping of Glutamate Receptor Proteins on Cultured Neuronal Cells Domestic conference

    細川 千絵, 岸本 龍典, 前澤 安代, 工藤 卓, 田口 隆久

    レーザー学会研究会報告 = Reports on topical meeting of the Laser Society of Japan  2016.11 

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  • Optical trapping dynamics of AMPA-type glutamate receptors on hippocampal neurons Domestic conference

    KISHIMOTO Tatsunori, MAEZAWA Yasuyo, KUDOH Suguru. N., TAGUCHI Takahisa, HOSOKAWA Chie

    計測自動制御学会ライフエンジニアリング部門シンポジウム論文集(CD-ROM)  2016.11 

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  • フェムト秒レーザー光刺激による神経回路網の誘発応答特性(Spatio-temporal activity pattern in neuronal network evaluated by femtosecond laser-induced stimulation) Domestic conference

    Fujioka Yuji, Nakagawa Yuta, Kudoh Suguru N., Taguchi Takahisa, Hosokawa Chie

    生物物理  2016.10  (一社)日本生物物理学会

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  • 顕微鏡の照射角制御によって最適化されたプラズモニックチップ上のナノ粒子の蛍光増強度 Domestic conference

    泉章太, 當麻真奈, 細川千絵, 田和圭子

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)  2016.09 

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  • 集光フェムト秒レーザーを用いた単一細胞刺激による神経細胞ネットワークの活動特性 Domestic conference

    中川裕太, 工藤卓, 田口隆久, 細川千絵

    応用物理学会春季学術講演会講演予稿集(CD-ROM)  2016.03 

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  • 神経細胞表面のグルタミン酸受容体分子の初期集合状態に依存した光捕捉過程 Domestic conference

    前澤安代, 岸本龍典, 工藤卓, 田口隆久, 細川千絵

    応用物理学会春季学術講演会講演予稿集(CD-ROM)  2016.03 

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  • Bull’s Eye構造のプラズモニックチップを用いた蛍光標識ナノ粒子の蛍光顕微鏡観察 Domestic conference

    泉章太, 細川千絵, 當麻真奈, 田和圭子

    応用物理学会春季学術講演会講演予稿集(CD-ROM)  2016.03 

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  • 集光レーザー摂動を用いた細胞内分子操作技術の開発 Domestic conference

    細川千絵

    第15回産総研・産技連LS-BT合同研究発表会要旨集,2016  2016 

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  • 培養神経細胞表面に局在するグルタミン酸受容体タンパク質の光補捉 Domestic conference

    細川千絵, 岸本龍典, 前澤安代, 工藤卓, 田口隆久

    レーザー学会研究会報告  2016 

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  • プラズモニックチップを用いた単一ナノ粒子の光捕捉過程の蛍光解析 Domestic conference

    宮内康平, 田和圭子, 工藤卓, 田口隆久, 細川千絵

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)  2015.08 

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  • Optical Trapping Dynamics of Glutamate Receptors in Cultured Neurons Domestic conference

    MAEZAWA Yasuyo, KUDOH Suguru N, TAGUCHI Takahisa, HOSOKAWA Chie

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)  2015.08 

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  • Sensitive Single‐Particle Fluorescence Imaging with a Plasmonic Chip Domestic conference

    TAWA Keiko, SASAKAWA Chisato, HOSOKAWA Chie, TAWA Keiko, SASAKAWA Chisato, HOSOKAWA Chie

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)  2015.08 

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  • フェムト秒レーザー光刺激による神経細胞ネットワークの機能解析(Functional analysis of living neuronal networks with a femtosecond laser-induced stimulation) Domestic conference

    Nakagawa Yuta, Kudoh Suguru N., Taguchi Takahisa, Hosokawa Chie

    生物物理  2015.08  (一社)日本生物物理学会

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  • 細胞を診て操作する生物物理的アプローチ 集光レーザー摂動を用いた神経回路網における分子ダイナミクスの直接操作(New biophysical approaches to explore and manipulate cells Direct manipulation of molecular dynamics in neuronal network with laser-induced perturbation) Domestic conference

    Hosokawa Chie

    生物物理  2015.08  (一社)日本生物物理学会

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  • プラズモニックディッシュで培養した神経細胞の高感度蛍光イメージング Domestic conference

    田和圭子, 藤田剛, 細川千絵, 西井準治

    高分子学会予稿集(CD-ROM)  2015.05 

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  • 光ピンセットを用いた神経細胞に局在する受容体タンパク質の捕捉・集合 Domestic conference

    MAEZAWA YASUYO, KUDO SUGURU, TAGUCHI TAKAHISA, HOSOKAWA CHIE

    応用物理学会春季学術講演会講演予稿集(CD-ROM)  2015.02 

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  • プラズモニックチップ上で培養した神経細胞接着分子の光捕捉 Domestic conference

    HOSOKAWA CHIE, MIYAUCHI KOHEI, KUDO SUGURU, TAGUCHI TAKAHISA, TAWA KEIKO

    応用物理学会春季学術講演会講演予稿集(CD-ROM)  2015.02 

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  • Root problem of image cognition : Spike code flow map in cultured neuronal networks Domestic conference

    TAMURA Shinichi, NISHITANI Yoshi, HOSOKAWA Chie, MIYOSHI Tomomitsu, SAWAI Hajime, KAMIMURA Takuya, YAGI Yasushi, MIZUNO-MATSUMOTO Yuko, CHEN Yen-Wei

    IEICE technical report.  2015.02 

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    It's important to investigate natural intelligence how the signal/information is flown in neuronal network. We observed spike trains produced by one-shot electrical stimulation with 8 × 8 multi-electrodes in cultured neuronal networks. Each electrode accepted spikes from several neurons. We then constructed code flow maps as movies of the electrode array to observe the code flow especially of "1101" and "1011." To quantify the flow, we calculated the cross-correlations of the maximum direction of the codes with lengths less than 8. Normalized cross-correlations in the maximum direction were almost constant irrespective of code. Thus, the analysis suggested that the local codes for electrode flow maintained the code shape to some extent and conveyed information in the neural network. Then we made a simulation of such code flow.

  • Laser-induced perturbation into molecular dynamics localized in neuronal cell Domestic conference

    Chie Hosokawa, Naoko Takeda, Suguru N. Kudoh, Takahisa Taguchi

    Progress in Biomedical Optics and Imaging - Proceedings of SPIE  2015.01 

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    © 2015 SPIE. Molecular dynamics at synaptic terminals in neuronal cells is essential for synaptic plasticity and subsequent modulation of cellular functions in a neuronal network. For realizing artificial control of living neuronal network, we demonstrate laser-induced perturbation into molecular dynamics in the neuronal cells. The optical trapping of cellular molecules such as synaptic vesicles or neural cell adhesion molecules labeled with quantum dots was evaluated by fluorescence imaging and fluorescence correlation spectroscopy. The trapping and assembling dynamics was revealed that the molecular motion was constrained at the focal spot of a focused laser beam due to optical trapping force. Our method has a potential to manipulate synaptic transmission at single synapse level.

  • 神経細胞表面分子の局所光操作に向けたプラズモン光ピンセット手法の開発 Domestic conference

    宮内康平, 田和圭子, 工藤卓, 田口隆久, 細川千絵

    電気学会電子・情報・システム部門大会講演論文集(CD-ROM)  2014.09 

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  • フェムト秒レーザー光刺激による神経細胞ネットワークの活動制御 Domestic conference

    中川裕太, 工藤卓, 田口隆久, 細川千絵

    電気学会電子・情報・システム部門大会講演論文集(CD-ROM)  2014.09 

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  • 1SDA-04 Laser-induced perturbation into living neuronal networks : Toward understanding neurodynamics(1SDA Measurement and control of neurodynamics,Symposium,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014)) Domestic conference

    Hosokawa Chie

    Biophysics  2014.08 

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  • Bright fluorescence imaging of cells cultured on the plasmonic dish Domestic conference

    Tawa Keiko, Fujita Tsuyoshi, Yasui Chikara, Kiyosue Kazuyuki, Hosokawa Chie, Sasakawa Chisato, Aota Hiroyuki, Kakinuma Norihiro, Oike Makoto, Nishii Junji

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY  2014.08 

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  • 神経ダイナミクスの計測と制御 神経ダイナミクス解明のためのレーザー摂動技術の開発(Measurement and control of neurodynamics Laser-induced perturbation into living neuronal networks: Toward understanding neurodynamics) Domestic conference

    Hosokawa Chie

    生物物理  2014.08  (一社)日本生物物理学会

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  • 表面プラズモン共鳴効果を利用した神経細胞接着分子の光捕捉 Domestic conference

    宮内康平, 田和圭子, 工藤卓, 田口隆久, 細川千絵

    ナノ学会大会講演予稿集  2014.05 

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  • 一体成型加工したプラズモニックディッシュ上で培養した神経細胞の蛍光顕微鏡イメージング Domestic conference

    藤田剛, 細川千絵, 西井準治, 柿沼憲宏, 尾池真, 田和圭子

    高分子学会予稿集(CD-ROM)  2014.05 

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  • 創薬スクリーニングに向けた細胞機能評価:集光レーザービームの光摂動を用いたアプローチ Domestic conference

    細川千絵

    日本化学会講演予稿集  2014.03 

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  • 光マニピュレーションの新しい生物科学応用 Domestic conference

    細川千絵

    日本化学会講演予稿集  2014.03 

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  • Root problem of image cognition : Hardware in brain Domestic conference

    Tamura Shinichi, Nishitani Yoshi, Hosokawa Chie, Miyoshi Tomomitsu, Sawai Hajime, Kamimura Takuya, Yagi Yasushi, Mizuno-Matsumoto Yuko, Chen Yen-Wei

    IEICE technical report.  2014.01 

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    Neuron is a simple computational element with rough function, e.g. it cannot receive spikes between refractory period of after it fires. Besides this, its parameters fluctuate time to time. However, as a neuron network they can work intellectually. This gap is an big problem to be solved not only from physiological point of view but also from computer science. We detected pseudo random sequences including M-sequence family in spike trains of cultured neuron network significantly more than by chance. This gave code flow map on multi-electrode array dishes (MED-P515A, Alpha MED Scientific, Kadoma, Osaka, Japan) with 8 × 8 planar microelectrodes. Each electrode catches spikes from several neurons. Conventionally these spike trains are sorted to extract spikes from respective single neuron. Unfortunately but fortunately, however, we obtained mixed neuron spike trains, and by analyzing these, we could find principle of intelligent neuronal network processing.

  • Clear images of neuronal cells cultured on a plasmonic dish observed with the inverted fluorescence microscope Domestic conference

    Keiko Tawa, Tsuyoshi Fujita, Tsuyoshi Fujita, Chie Hosokawa, Junji Nishii

    Optics InfoBase Conference Papers  2013.12 

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  • Laser manipulation of intracellular molecular dynamics in hippocampal neurons Domestic conference

    C. Hosokawa, Y. Ueda, N. Takeda, S. N. Kudoh, T. Taguch

    Pacific Rim Conference on Lasers and Electro-Optics, CLEO - Technical Digest  2013.10 

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    We demonstrated laser manipulation of intracellular molecular dynamics in neurons to realize artificial control toward modulating synaptic transmission in a neuronal network. The optical trapping and assembling dynamics of synaptic vesicles and neural cell adhesion molecules in neurons was revealed by fluorescence analysis. © 2013 IEEE.

  • Optical perturbation of intracellular molecular dynamics of single neuron in living neuronal network Domestic conference

    HOSOKAWA Chie, TAKEDA Naoko, UEDA Yusuke, KUDOH Suguru N, TAGUCHI Takahisa, TAGUCHI Takahisa

    生物物理  2013.09 

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  • Spatio-temporal dynamics of spontaneous activity in living neuronal network by femtosecond laser-induced cutting of neurites Domestic conference

    KUBO Hayato, KUDOH Suguru N, TAGUCHI Takahisa, HOSOKAWA Chie

    生物物理  2013.09 

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  • Evaluation of Functional Connectivity in Hippocampal Neuronal Network with Femtosecond-Laser Cutting of Neurites Domestic conference

    KUBO Hayato, KUDOH Suguru N., TAGUCHI Takahisa, HOSOKAWA Chie

    計測自動制御学会ライフエンジニアリング部門シンポジウム論文集(CD-ROM)  2013.09 

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  • 共鳴レーザー照射による神経細胞接着分子の光捕捉 Domestic conference

    武田尚子, 工藤卓, 田口隆久, 細川千絵

    電気学会電子・情報・システム部門大会講演論文集(CD-ROM)  2013.09 

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  • 集光レーザービームの光摂動による神経細胞内分子動態の集合操作(Optical perturbation of intracellular molecular dynamics of single neuron in living neuronal network) Domestic conference

    Hosokawa Chie, Takeda Naoko, Ueda Yusuke, Kudoh Suguru N., Taguchi Takahisa

    生物物理  2013.09  (一社)日本生物物理学会

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  • フェムト秒レーザー神経突起切断による神経回路網の自発活動の時空間ダイナミクス(Spatio-temporal dynamics of spontaneous activity in living neuronal network by femtosecond laser-induced cutting of neurites) Domestic conference

    Kubo Hayato, Kudoh Suguru N., Taguchi Takahisa, Hosokawa Chie

    生物物理  2013.09  (一社)日本生物物理学会

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  • 神経細胞接着分子の光捕捉過程における共鳴レーザー照射効果 Domestic conference

    武田尚子, 工藤卓, 田口隆久, 細川千絵

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)  2013.08 

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  • Clear Images of Neuronal Cells Cultured on a Plasmonic Dish Observed with the Inverted Fluorescence Microscope Domestic conference

    TAWA KEIKO, FUJITA TSUYOSHI, HOSOKAWA CHIE, NISHII JUNJI

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)  2013.08 

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  • プラズモニックチップを用いた神経細胞の倒立落射蛍光顕微鏡イメージング Domestic conference

    藤田剛, 細川千絵, 西井準治, 田和圭子

    高分子学会予稿集(CD-ROM)  2013.08 

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  • Optical trapping and assembling dynamics of intracellular molecules in naneuronal cells Domestic conference

    Hosokawa Chie

    Meeting abstracts of the Physical Society of Japan  2013.08 

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  • 光集合制御による神経細胞内分子動態の解明 Domestic conference

    細川千絵

    日本物理学会講演概要集  2013.08 

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  • 蛍光イメージングへの応用を目指したプラズモニックチップの構造と光学特性評価 Domestic conference

    藤田剛, 安井力, 細川千絵, 西井準治, 青田浩幸, 田和圭子

    高分子学会予稿集(CD-ROM)  2013.05 

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  • 光ピンセットによる神経細胞内分子動態の集合操作 Domestic conference

    細川千絵, 武田尚子, 植田悠介, 工藤卓, 田口隆久

    応用物理学会春季学術講演会講演予稿集(CD-ROM)  2013.03 

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  • フェムト秒レーザーを用いた神経突起切断による神経回路網の機能解析 Domestic conference

    久保勇人, 工藤卓, 田口隆久, 細川千絵

    応用物理学会春季学術講演会講演予稿集(CD-ROM)  2013.03 

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  • Fluorescence Imaging of Neuron Cells Cultured on a Plasmonic Dish Domestic conference

    TAWA KEIKO, YASUI CHIKARA, HOSOKAWA CHIE, NISHII JUNJI, AOTA HIROYUKI

    Abstr JSAP MRS Jt Symp (CD-ROM)  2013 

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  • M-sequence family from cultured neural circuits Domestic conference

    Shinichi Tamura, Yoshi Nishitani, Chie Hosokawa, Yuko Mizuno-Matsumoto, Takuya Kamimura, Yen Wei Chen, Tomomitsu Miyoshi, Hajime Sawai

    Proceedings - 2012 6th International Conference on New Trends in Information Science, Service Science and Data Mining (NISS, ICMIA and NASNIT), ISSDM 2012  2012.12 

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    We have already shown its feasibility by computer simulations that show a loop circuit can be copied to another area by back-propagation leaning algorithm; this is a key function of brain intelligence, which includes functions such as memory, association, reasoning, and abstraction. We also have found M-sequences in spike trains of cultured neuronal networks. In this paper, we report that we found family or fragment of M-sequence responses also in the instantaneous firing rates (PSTH's) from cultured neural networks of published data and our own data, which suggest the existence of loop circuits with a feedback link composed of 3 to 5 neuron cells or equivalent circuits. © 2012 AICIT.

  • Optical trapping and assembling of neural cell adhesion molecules in living neuronal cells Domestic conference

    TAKEDA N., KUDOH S. N., TAGUCH T., HOSOKAWA C.

    電気学会研究会資料. MBE, 医用・生体工学研究会  2012.09 

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  • 集光フェムト秒レーザーを用いた単一神経細胞の光刺激法の開発 Domestic conference

    坂本泰隆, 工藤卓, 田口隆久, 細川千絵

    電気学会電子・情報・システム部門大会講演論文集(CD-ROM)  2012.09 

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  • 光捕捉された神経細胞内シナプス小胞群の集合ダイナミクス(Assembling dynamics of optically trapped synaptic vesicles in neuronal cell) Domestic conference

    Ueda Yusuke, Kudoh Suguru N., Taguchi Takahisa, Hosokawa Chie

    生物物理  2012.08  (一社)日本生物物理学会

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  • Bright Fluorescence Images of Neurons Cultured on a Plasmonic Chip with Upright-Inverted Microscope Domestic conference

    YASUI Chikara, TAWA Keiko, HOSOKAWA Chie, NISHII Junji, AOTA Hiroyuki

    応用物理学会学術講演会講演予稿集(CD-ROM)  2012.08 

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  • Detection of M-sequences from cell cultures Domestic conference

    NISHITANI Yoshi, HOSOKAWA Chie, MIZUNO Matsumoto Yuko, MIYOSHI Tomomitsu, SAWAI Hajime, TAMURA Shin-ichi

    IEICE technical report. Neurocomputing  2012.07 

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    We consider that the memory is composed of feedback shift register (FSR) circuit in the cerebral cortex. In this study, we tried to detect M-sequences known as a pseudo random sequence generated by the LFSR circuit from time series patterns of stimulated action potentials of cultured neuronal networks. We could found several M-sequences from a 3-stage LFSR circuit (M3). Then neuronal networks have not a clock control system as a calculator, therefore the bin size of detected M sequences were modulated. We call this phenomenon margin, We tried to test the correlation between margin and number of detected M3. As a result, we found a positive coefficient of correlation.

  • 光ピンセットによる神経細胞接着分子の動態制御 Domestic conference

    武田尚子, 細川千絵, 工藤卓, 田口隆久

    ナノ学会大会講演予稿集  2012.06 

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  • プラズモニックチップを用いた神経細胞の高感度蛍光顕微鏡イメージング Domestic conference

    安井力, 田和圭子, 細川千絵, 西井準治, 青田浩幸, 松本昭

    高分子学会予稿集(CD-ROM)  2012.05 

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  • プラズモニックチップ上で培養した神経細胞の高感度蛍光顕微鏡観察 Domestic conference

    安井力, 田和圭子, 細川千絵, 西井準治, 青田浩幸, 松本昭

    日本化学会講演予稿集  2012.03 

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  • プラズモニックチップを用いた高感度蛍光イメージング Domestic conference

    田和圭子, 安井力, 細川千絵, 西井準治, 青田浩幸

    関西大学先端科学技術シンポジウム講演集  2012.01 

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  • 8G16 Fluorescence analysis of manipulation dynamics of neuronal network under laser perturbation Domestic conference

    HOSOKAWA Chie, UEDA Yusuke, SAKAMOTO Yasutaka, ONISHI Eriko, KUDOH Suguru N., TAGUCHI Takahisa

    バイオエンジニアリング講演会講演論文集  2012.01 

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  • Optical Perturbation of Neuronal Network by a Focused Laser Beam Domestic conference

    HOSOKAWA C., UEDA Y., SAKAMOTO Y.,SUZUKI M.,KUDOH S. N.,TAGUCHI T.

    生体・生理工学シンポジウム論文集(CD-ROM))  2011.09 

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  • 光ピンセットによる神経細胞内シナプス小胞群の集合操作の蛍光解析 Domestic conference

    植田悠介, 細川千絵, 工藤卓, 田口隆久

    応用物理学会学術講演会講演予稿集(CD-ROM)  2011.08 

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  • CdSe/ZnS量子ドット分散ガラスビーズを添加した神経回路網の細胞外電位多点計測による毒性評価 Domestic conference

    細川千絵, 大西映里子, 工藤卓, 村瀬至生, 安藤昌儀, 川崎一則, 田口隆久

    応用物理学会学術講演会講演予稿集(CD-ROM)  2011.08 

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  • 神経細胞内シナプス小胞群の光捕捉ダイナミクス Domestic conference

    細川千絵, 植田悠介, 工藤卓, 田口隆久

    ナノ学会大会講演予稿集  2011.06 

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  • CdSe/ZnS量子ドット分散ガラスビーズを添加した神経回路網の機能解析 Domestic conference

    細川千絵, 鈴木真理子, 工藤卓, 村瀬至生, 安藤昌儀, 川崎一則, 川田口隆久

    ナノ学会大会講演予稿集  2011.06 

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  • 光捕捉された神経細胞内シナプス小胞群の蛍光相関分光解析 Domestic conference

    細川千絵, 植田悠介, 工藤卓, 大西映里子, 鈴木真理子, 田口隆久

    応用物理学関係連合講演会講演予稿集(CD-ROM)  2011.03 

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  • Analyzing read-out pattern from brain neuronal network based on loop circuit hypothesis Domestic conference

    NISHITANI YOUSHI, HOSOKAWA CHIE, TAMURA SHIN'ICHI

    電子情報通信学会技術研究報告  2011.02 

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    We consider that the memory is composed of loop neural circuit in the cerebral cortex. We have already shown a feasibility of self organization of loop neural circuit for memory, association, and abstraction in our previous study. However, it remains unclear that how the memory is stored and transferred in real neural circuit. In this study, we found pseudo random sequences from cultured neuron networks, which suggest the existence of loop circuit with 3 elements and feedback link.

  • Memory on a chip Domestic conference

    Hidekatsu Ito, Chie Hosokawa, Suguru N. Kudoh

    2010 International Symposium on Micro-NanoMechatronics and Human Science: From Micro and Nano Scale Systems to Robotics and Mechatronics Systems, MHS 2010, Micro-Nano GCOE 2010, Bio-Manipulation 2010  2010.12 

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    The rat hippocampal neurons were cultured on a dish with 64 planer micro electrodes array. Neurons reorganized a functional network, and an external inputs form outer world elicited a reproducible, particular spatiotemporal pattern of evoked action potentials. The patterns were not completely uniform for repeptive stimulation but varied slightly. We previously reported that dissociated rat hippocampal neurons possessed hysterical function in its network dynamics. The spatio-temporal electrical activity of the living neuronal network evoked by paired stimulation is quite different from one evoked by a single stimulation, suggested that the network can "memorize" an existence of precedent stimulation. In this report, the feature of this type of network hysteresis was investigated and the hysteresis was kept for at least 1s and not kept for 10s. In addition, this hysteresis was remarkable only in aged cultures with matured complex network. Network hysteresis depends on internal state of the network, generated by the autonomous activity in self-organized networks. ©2010 IEEE.

  • Morphology-tunable Strongly Emitting Fibers Self-assembled from Silica-coated CdTe QDs Domestic conference

    MURASE NORIO, YANG PING, ANDO MASANORI, KAWASAKI KAZUNORI, KATO TOMOKI, HOSOKAWA CHIE, TAGUCHI TAKAHISA

    日本セラミックス協会秋季シンポジウム講演予稿集  2010.11 

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  • Preparation of glass beads incorporating quantum dots for use as fluorescent reagent and neuronal cell imaging Domestic conference

    ANDO Masanori, LI Chunliang, YANG Ping, MURASE Norio, HOSOKAWA Chie, TAGUCHI Takahisa

    IEICE technical report.  2010.11 

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    Quantum dots (QDs) show bright photoluminescence whose emission wavelength can be controlled by their particle size and composition. Compared with organic fluorescent dyes, QDs are more resistant to light irradiation. Because of those advantages, QDs attract much interest as novel bright fluorescent reagents. Photoluminescent QDs are synthesized in solution. However, QDs in solution are not very stable and tend to degradate. Therefore, we developed techniques to incorporate multiple QDs such as CdSe and InP in a small glass bead (diameter: several tens nm) by using sol-gel method at room temperature and under atmospheric pressure. The QD-incorporating glass beads are water-dispersible and emit bright photoluminescence. When dispersed in buffer solution for biological use, the glass beads showed much higher stability and light-resistance than commercial polymer-coated QDs. It was possible to obtain high-resolution fluorescence image of living neuronal cells by introducing the glass beads in the cells by endocytosis.

  • Preparation of glass beads incorporating quantum dots for use as fluorescent reagent and neuronal cell imaging Domestic conference

    ANDO Masanori, LI Chunliang, YANG Ping, MURASE Norio, HOSOKAWA Chie, TAGUCHI Takahisa

    IEICE technical report.  2010.11 

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    Quantum dots (QDs) show bright photoluminescence whose emission wavelength can be controlled by their particle size and composition. Compared with organic fluorescent dyes, QDs are more resistant to light irradiation. Because of those advantages, QDs attract much interest as novel bright fluorescent reagents. Photoluminescent QDs are synthesized in solution. However, QDs in solution are not very stable and tend to degradate. Therefore, we developed techniques to incorporate multiple QDs such as CdSe and InP in a small glass bead (diameter: several tens nm) by using sol-gel method at room temperature and under atmospheric pressure. The QD-incorporating glass beads are water-dispersible and emit bright photoluminescence. When dispersed in buffer solution for biological use, the glass beads showed much higher stability and light-resistance than commercial polymer-coated QDs. It was possible to obtain high-resolution fluorescence image of living neuronal cells by introducing the glass beads in the cells by endocytosis.

  • 疎水性量子ドットを分散した高輝度蛍光を示す微小ガラスカプセルの作製と応用 Domestic conference

    村瀬至生, 楊萍, 安藤昌儀, 鈴木真理子, 細川千絵, 川崎一則, 加藤智樹, 上垣浩一, 田口隆久, 田口隆久

    応用物理学会学術講演会講演予稿集(CD-ROM)  2010.08 

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  • 疎水性量子ドットを分散した高輝度蛍光を示す微小ガラスカプセルの作製と応用 Domestic conference

    村瀬至生, 楊萍, 安藤昌儀, 鈴木真理子, 細川千絵, 川崎一則, 加藤智樹, 上垣浩一, 田口隆久, 田口隆久

    応用物理学会学術講演会講演予稿集(CD-ROM)  2010.08 

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  • 光ピンセットを用いた培養神経細胞シナプス操作過程の蛍光解析(Fluorescence analysis of optical trapping dynamics of synaptic vesicles in dissociated neuron) Domestic conference

    細川 千絵, 大西 映里子, 工藤 卓, 田口 隆久

    神経化学  2010.08 

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  • CdSe/ZnS量子ドット分散ガラスビーズの神経細胞内単一粒子発光計測 Domestic conference

    細川千絵, 大西映里子, 村瀬至生, 安藤昌儀, 川崎一則, 田口隆久

    応用物理学会学術講演会講演予稿集(CD-ROM)  2010.08 

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  • 多数のCdSe/ZnS量子ドットを分散した高輝度ガラスビーズの作製と評価 Domestic conference

    村瀬至生, PING Yang, 鈴木真理子, 細川千絵, 川崎一則, 加藤智樹, 上垣浩一, 田口隆久

    ナノ学会大会講演予稿集  2010.05 

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  • InP量子ドット分散蛍光ガラスビーズの作製と神経細胞イメージング Domestic conference

    安藤昌儀, LI Chunliang, 細川千絵, 鈴木真理子, 村瀬至生, 川崎一則, 田口隆久

    ナノ学会大会講演予稿集  2010.05 

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  • CdSe/ZnS量子ドット分散ガラスビーズを用いた神経細胞の発光解析 Domestic conference

    細川千絵, 大西映里子, 村瀬至生, 安藤昌儀, 川崎一則, 田口隆久

    ナノ学会大会講演予稿集  2010.05 

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  • 刺激応答履歴性を利用したニューロ・ロボット Domestic conference

    徳田農, 伊東嗣功, 細川千絵, 工藤卓

    電気学会電子・情報・システム部門大会講演論文集(CD-ROM)  2010 

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  • ガラスコートInP量子ドットを用いた神経細胞の蛍光イメージング Domestic conference

    安藤昌儀, 細川千絵, 鈴木真理子, 田口隆久

    応用物理学会学術講演会講演予稿集  2009.09 

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  • 3S11p05 "Vitroid", Neuro-Robot as testing tube for Neuroengineering Domestic conference

    KONDOH Suguru N., KIYOHARA Ai, TOKUDA Minori, HOSOKAWA Chie, TAGUCHI Takahisa

    日本生物工学会大会講演要旨集  2009.08 

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  • 生物と機械の融合 バイオロボティクス "試験管"としてのニューロ・ロボット"Vitroid" Domestic conference

    工藤 卓, 清原 藍, 徳田 農, 細川 千絵, 田口 隆久

    日本生物工学会大会講演要旨集  2009.08  (公社)日本生物工学会

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  • 多点電極アレイ上の神経細胞パターニング Domestic conference

    細川千絵, 工藤卓, 鈴木真理子, 清原藍, 細川陽一郎, 岡野和宣, 増原宏, 田口隆久

    応用物理学関係連合講演会講演予稿集  2009.03 

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  • Vitroid - a robot with link between living neuronal network in vitro and robot body Domestic conference

    Suguru N. Kudoh, Minori Tokuda, Ai Kiyohara, Chie Hosokawa, Takahisa Taguchi, Isao Hayashi

    Proceedings of 2008 IEEE International Conference on Mechatronics and Automation, ICMA 2008  2008.12 

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    Rat hippocampal neurons organized complex networks on a culture dish which has 64 planar microelectrodes and the spontaneous action potentials were frequently observed. The living neuronal network was able to distinguish patterns of action potentials evoked by different inputs, suggesting that a cultured neuronal network can represent and process particular states as symbols. We use a Khepera II robot and a robot made by LEGO mindstorm NXT kit as a robot body for interfacing with a living neuronal network and the outer world. We call the system "Vitroid". Vitroid has the living neurons, robot body, and direct coupling type of controllers to interface neurons with the robot. We succeeded in performing obstacle avoidance behavior with premised control rule sets. Using self-tuning fuzzy reasoning, we associated a distinct spatial pattern of electrical activity with a particular phenomenon in the outside of the culture dish. © 2008 IEEE.

  • 量子ドット分散ガラスビーズを用いた神経細胞イメージング Domestic conference

    細川千絵, 安藤昌儀, 清原藍, 工藤卓, 田口隆久

    応用物理学会学術講演会講演予稿集  2008.09 

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  • 量子ドット分散ガラスビーズを用いた神経細胞イメージング Domestic conference

    細川千絵, 安藤昌儀, 清原藍, 工藤卓, 田口隆久

    応用物理学会学術講演会講演予稿集  2008.09 

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  • Optical Trapping of Synaptic Vesicles in Neuronal Cells Domestic conference

    HOSOKAWA CHIE, KUDO SUGURU, KIYOHARA AI, TAGUCHI TAKAHISA

    生体・生理工学シンポジウム論文集  2008.09 

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  • Vitroid as a test tube-in vitro learning in neuro-robot system. Domestic conference

    KUDO SUGURU, TOKUDA MINORI, KIYOHARA AI, KIYOHARA AI, HOSOKAWA CHIE, HAYASHI ISAO, TAGUCHI TAKAHISA, TAGUCHI TAKAHISA

    生体・生理工学シンポジウム論文集  2008.09 

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  • 光ピンセットによる神経細胞内シナプス小胞群の捕捉過程 Domestic conference

    細川千絵, 工藤卓, 清原藍, 田口隆久

    応用物理学関係連合講演会講演予稿集  2008.03 

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  • Resynchronization in Living Neuronal Network Divided by a Focused Femtosecond Laser Domestic conference

    HOSOKAWA C.,KUDOH S.N., KIYOHARA A.,TAGUCHI T.

    生体・生理工学シンポジウム論文集  2008 

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  • Development of heterogeneity of functional synaptic connection in dissociated neurons Domestic conference

    KUDOH S.N, KIYOHARA A, HOSOKAWA C, TAGUCHI T

    生体・生理工学シンポジウム論文集  2008 

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  • Interaction between living neuronal network and outer world by programmable multisite stimulation system Domestic conference

    Suguru N. Kudoh, Ai Kiyohara, Chie Hosokawa, Takahisa Taguchi, Isao Hayashi

    2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS  2007.12 

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    Rat hippocampal neurons organized complex networks on a culture dish which has 64 planner microelectrodes and action potentials of neurons were recorded from an individual electrode. MED64 system, one of multi site recording systems for extracellular action potential was used for recording network activities of living neuronal networks and for applying inputs from outer world to the network. Neurons reconstructed complicated networks along culture days, and a spatio-temporal pattern of spontaneous action potentials was drastically changed after transient high-frequency bursting activities (HFB) . After HFB, spatial distribution of spontaneous action potentials changed to heterogeneous one. We designed programmable multisite stimulation head amplifier, and analysis of evoked action potentials induced by the stimulator confirmed that there are some hub-like structure which has many inputs from other neurons. In addition we are developing "biomodeling system", in which living neuronal network connected to moving robot. ©2007 IEEE.

  • 1P210 Vesicle dynamics of hippocampal synapses in optical trapping(Neural network and brain computing,Poster Presentations) Domestic conference

    Hosokawa Chie, Kudoh Suguru N., Kiyohara Ai, Taguchi Takahisa

    Biophysics  2007.11 

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  • 光捕捉による海馬神経シナプスの小胞ダイナミクス(Vesicle dynamics of hippocampal synapses in optical trapping) Domestic conference

    細川 千絵, 工藤 卓, 清原 藍, 田口 隆久

    生物物理  2007.11  (一社)日本生物物理学会

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  • フェムト秒レーザーにより切断された海馬神経細胞回路網における機能的再結合(Functional regeneration of hippocampal neurons on multielectrode array dish controlled by femtosecond laser processing) Domestic conference

    Hosokawa Chie, Kiyohara Ai, Kudoh Suguru N., Taguchi Takahisa

    神経化学  2007.08  (一社)日本神経化学会

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  • Recognition of outer world performed by dissociated neurons. Domestic conference

    KIYOHARA AI, KUDO SUGURU, TOKUDA MINORI, HOSOKAWA CHIE, TAGUCHI TAKAHISA, HAYASHI ISAO

    インテリジェント・システム・シンポジウム講演論文集  2007.08 

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  • 集光フェムト秒レーザーによる分散培養神経回路網の微細加工 Domestic conference

    細川千絵, 工藤卓, 清原藍, 田口隆久

    応用物理学関係連合講演会講演予稿集  2007.03 

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  • 蛍光相関分光で捉えた光圧ポテンシャル内でのナノ粒子会合ダイナミクス Domestic conference

    吉川裕之, 細川千絵, 増原宏

    蛍光相関分光で見る生体系の情報伝達4 理研シンポジウム 平成19年  2007 

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  • Optical trapping and fluorescence analysis of thermo-responsive poly(N-alkylacrylamide) microgel particles in water Domestic conference

    Yuriko Matsumura, Chie Hosokawa, Hiroyuki Yoshikawa, Kaoru Iwai, Hiroshi Masuhara

    Polymer Preprints, Japan  2006.10 

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    When laser beam was focused into nanoparticle suspensions, nanoparticles can be trapped in the focal spot due to optical gradient force. Here we report the optical trapping and fluorescence analysis of poly(N-isopropylacrylamide) (PNIPAM) and its related polymer microgel particles which were labeled with 4-N-(2-acryloyloxyethyl)-N-methylamino-7-N,N-dimethylaminosulfonyl-2,1, 3-benzoxadiazole (DBD-AE) dispersed in water. The DBD-AE labeled PNIPAM microgel particles were trapped at the focal point by irradiation with cw-Nd 3+:YAG laser (λ = 1064 nm) in water and the fluorescence intensity of the DBD-AE labeled PNIPAM microgel particles excited by diode laser (λ = 405 nm) increased stepwise. We succeeded to fluorescence analysis of trapped single microgel particle.

  • 単一ポリ(N‐イソプロピルアクリルアミド)ゲル微粒子の光圧効果の蛍光解析 Domestic conference

    細川千絵, 松村有里子, 吉川裕之, 岩井薫, 増原宏

    光化学討論会講演要旨集  2006.09 

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  • 光圧による液中ナノ粒子の捕捉・集合機構の解明 Domestic conference

    細川千絵, 吉川裕之, 増原宏

    応用物理学会学術講演会講演予稿集  2006.08 

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  • 感熱応答性ポリアクリルアミドゲル微粒子の光捕捉と蛍光解析 Domestic conference

    松村有里子, 細川千絵, 吉川裕之, 岩井薫, 増原宏

    高分子学会予稿集(CD-ROM)  2006.05 

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  • 光圧による液中ナノ粒子の集合ダイナミクス Domestic conference

    細川千絵, 吉川裕之, 増原宏

    応用物理学関係連合講演会講演予稿集  2006.03 

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  • 光圧を利用したナノ粒子の捕捉・集合・配列 Domestic conference

    吉川裕之, 細川千絵, 増原宏

    コロイドおよび界面化学討論会講演要旨集  2004.08 

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  • 単一粒子レベルでみた光圧による高分子ナノ粒子の集合過程(II) Domestic conference

    江田千絵, 吉川裕之, 増原宏

    コロイドおよび界面化学討論会講演要旨集  2003.08 

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  • 単一粒子レベルでみた光圧による高分子ナノ粒子の集合過程 Domestic conference

    江田千絵, 吉川裕之, 増原宏

    コロイドおよび界面化学討論会講演要旨集  2002.09 

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  • 光圧による高分子ナノ微粒子凝集過程の二光子励起蛍光解析(II) Domestic conference

    江田千絵, 吉川裕之, 増原宏

    応用物理学会学術講演会講演予稿集  2001.09 

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  • 光圧による高分子ナノ微粒子凝集過程の二光子励起蛍光解析 Domestic conference

    江田千絵, 吉川裕之, 増原宏

    応用物理学会学術講演会講演予稿集  2000.09 

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  • 光圧による神経細胞シナプス機能分子の操作と細胞機能制御への応用 Invited Domestic conference

    2000.05 

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Industrial Property Rights

Grant-in-Aid for Scientific Research

  • レーザー誘起力学摂動による神経回路網の機能制御

    Grant-in-Aid for Scientific Research(B)  2023.04

  • レーザー刺激による神経細胞間シナプス伝達強度の時空間操作

    Grant-in-Aid for Challenging Research (Pioneering)/(Exploratory)  2023.04

  • 超螺旋光による生体組織への刺激と自己組織化反応

    Grant-in-Aid for Transformative Research Areas  2022.07

  • Optical control of neuro-dynamics with laser-induced molecular manipulation

    Grant-in-Aid for Scientific Research(B)  2017

  • Theories, metrologies, and observations of optical forces for establishing basics of optical manipulation

    Grant-in-Aid for Scientific Research on Innovative Areas  2016.06

  • Bull’s eyeパターン化プラズモニックチップによる神経細胞ネットワーク解析

    Grant-in-Aid for Scientific Research(A)  2016.04

  • Code distribution map of neuronal network

    Grant-in-Aid for Challenging Research (Pioneering)/(Exploratory)  2013.04

  • 集光レーザー摂動による神経細胞ネットワークダイナミクスの解明

    Grant-in-Aid for Young Scientists(A)  2013

  • 表面プラズモン増強効果を利用した細胞内分子マニピュレーション手法の開発

    Grant-in-Aid for Challenging Research (Pioneering)/(Exploratory)  2013

  • プラズモン増強効果を示す量子ドット分散微小球のゾル-ゲル法を駆使した作製と評価

    Grant-in-Aid for Scientific Research(C)  2012.04

  • スイッチング可能な光-分子強結合場のための蛍光性高分子の創製

    Grant-in-Aid for Scientific Research on Priority Areas  2009

  • 共鳴レーザー光を利用した細胞内ナノマニピュレーション

    Grant-in-Aid for Young Scientists(B)  2009

  • マルチスケール神経配置操作による神経回路網環境応答特性制御

    Grant-in-Aid for Scientific Research on Priority Areas  2008

  • Neural information decoding by estimation of the dynamic functional connectivity

    Grant-in-Aid for Scientific Research(A)  2007

  • 単一粒子レベルでみた光圧による溶液中ナノ粒子の凝集ダイナミクス

    Grant-in-Aid for JSPS Fellows  2003

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Contract research

  • レーザー摂動を用いた細胞内分子操作による神経情報処理機構の解明

    国立研究開発法人 科学技術振興機構  創発的研究支援事業  2021.04

  • レーザー誘起光集合制御による神経細胞内分子動態の時空間ダイナミクスの解明

    科学技術振興機構  戦略的創造研究推進事業 さきがけ研究  2006

Charge of on-campus class subject

  • 化学反応論1

    2023   Weekly class   Undergraduate

  • 化学産業論

    2023   Weekly class   Graduate school

  • 特別研究

    2023   Intensive lecture   Undergraduate

  • 物理化学特論B

    2023   Weekly class   Graduate school

Charge of off-campus class subject

  • 熱力学

    2020.04
    -
    2022.03
    Institution:Osaka City University

  • 基礎分析化学

    Institution:Osaka City University

  • 特別講義(神経情報学)

    Institution:Kansai University

Job title

  • Job title within the department

    Graduate School of Science Department of Chemistry 

    専攻長  2022.04 - 2023.03

  • Job title within the department

    School of Science Department of Chemistry 

    学科長  2022.04 - 2023.03

Other

  • Job Career

    2019.01 - Now

      More details

    Osaka City University