Updated on 2024/03/26

写真a

 
Nishimura Shigenori
 
Organization
Graduate School of Agriculture Department of Applied Biological Chemistry Assistant Professor
School of Agriculture Department of Applied Biological Chemistry
Title
Assistant Professor
Affiliation
Institute of Agriculture
Affiliation campus
Nakamozu Campus

Position

  • Graduate School of Agriculture Department of Applied Biological Chemistry 

    Assistant Professor  2022.04 - Now

  • School of Agriculture Department of Applied Biological Chemistry 

    Assistant Professor  2022.04 - Now

Degree

  • 修士(理学) ( Others )

Research Areas

  • Life Science / Structural biochemistry

  • Life Science / Applied biochemistry

Research subject summary

  • 微生物由来アミラーゼの立体構造と機能の研究

  • 寄生性原虫由来酵素の構造と機能の解析および薬剤開発への展開

  • NMRによる生理活性ペプチドの構造解析

Research Career

  • 微生物由来アミラーゼの立体構造と機能の研究

    β-アミラーゼ、X線結晶構造解析  Joint Research in Organization

    2014.04 - Now 

  • 寄生性原虫由来酵素の構造と機能の解析および薬剤開発への展開

    トリパノソーマ症、X線結晶構造解析、GMPレダクターゼ 

    2014.04 - Now 

Professional Memberships

  • 日本核磁気共鳴学会

    2014.10 - Now   Domestic

  • 日本応用糖質科学会

    2005.07 - Now

  • 日本蛋白質科学会

    2002.05 - Now

  • 日本農芸化学会

    1995.05 - Now

  • 日本生物物理学会

    1990.11 - Now

Papers

  • Colloidal dispersion of chiral layered hydroxide salt (LHS) nanocrystals exhibiting chiroptical response Reviewed

    Yasuaki Tokudome, Akihiro Koyama, Hidenobu Murata, Kenji Okada, Atsushi Nakahira, Shigenori Nishimura, Masahide Takahashi

    Journal of Sol-Gel Science and Technology   2022.04

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    Publishing type:Research paper (scientific journal)  

  • Structure-based prediction of the IgE epitopes of the major dog allergen Can f 1.

    Nakatsuji M, Sugiura K, Suda K, Sakurai M, Ubatani M, Muroya H, Okubo R, Noguchi R, Kamata Y, Fukutomi Y, Ishibashi O, Nishimura S, Inui T

    The FEBS journal   289 ( 6 )   1668 - 1679   2022.03( ISSN:1742-464X

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  • Allosteric regulation accompanied by oligomeric state changes of Trypanosoma brucei GMP reductase through cystathionine-β-synthase domain

    Imamura A, Okada T, Mase H, Otani T, Kobayashi T, Tamura M, Kubata BK, Inoue K, Rambo RP, Uchiyama S, Ishii K, Nishimura S, Inui T.

    Nat. Commun. 雑誌   11 ( 1 )   2020.04

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    Kind of work:Joint Work  

  • Structures and chitin-binding properties of two N-terminal lysin motifs (LysMs) found in a chitinase from Volvox carteri. Reviewed

    Kitaoku Y, Nishimura S, Hirono T, Suginta W, Ohnuma T, Fukamizo T.

    Glycobiology 雑誌   29 ( 7 )   2019.07

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    Kind of work:Joint Work  

  • Crystal structure of the dog allergen Can f 6 and structure-based implications of its cross-reactivity with the cat allergen Fel d 4. Reviewed

    Yamamoto K, Ishibashi O, Sugiura K, Ubatani M, Sakaguchi M, Nakatsuji M, Shimamoto S, Noda M, Uchiyama S, Fukutomi Y, Nishimura S, Inui T.

    Scientific Reports 雑誌   9 ( 1 )   2019.02

  • Crystal structure of exo-rhamnogalacturonan lyase from Penicillium chrysogenum as a member of polysaccharide lyase family 26. Reviewed

    Kunishige Y, Iwai M, Nakazawa M, Ueda M, Tada T, Nishimura S, Sakamoto T.

    FEBS Lett. 雑誌   592 ( 8 )   2018.04

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    Kind of work:Joint Work  

  • Mechanism of chitosan recognition by CBM32 carbohydrate-binding modules from a Paenibacillus sp. IK-5 chitosanase/glucanase. Reviewed

    Shinya S, Nishimura S, Kitaoku Y, Numata T, Kimoto H, Kusaoke H, Ohnuma T, Fukamizo T.

    Biochem J. 雑誌   473 ( 8 )   2016.04

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    Kind of work:Joint Work  

  • Characterization of the novel Trypanosoma brucei inosine 5'-monophosphate dehydrogenase. Reviewed

    Bessho T, Morii S, Kusumoto T, Shinohara T, Noda M, Uchiyama S, Shuto S, Nishimura S, Djikeng A, Duszenko M, Martin SK, Inui T, Kubata KB.

    Parasitology   140 ( 6 )   735 - 45   2013.05

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    Kind of work:Joint Work  

  • Drug delivery system for poorly water-soluble compounds using lipocalin-type prostaglandin D synthase. Reviewed

    Fukuhara A, Nakajima H, Miyamoto Y, Inoue K, Kume S, Lee YH, Noda M, Uchiyama S, Shimamoto S, Nishimura S, Ohkubo T, Goto Y, Takeuchi T, Inui T.

    J Control Release 雑誌   159 ( 1 )   143 - 50   2012.04

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    Kind of work:Joint Work  

  • NMR and CD analysis of an intermediate state in the thermal unfolding process of mouse lipocalin-type prostaglandin D synthase. Reviewed

    Miyamoto Y, Noda Y, Iida T, Yamaguchi K, Nishimura S, Tanaka A, Segawa S, Inui T.

    J Biochem 雑誌   151 ( 3 )   335 - 42   2012.03

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    Kind of work:Joint Work  

  • A rapid and simple method of evaluating the dimeric tendency of fluorescent proteins in living cells using a truncated protein of importin α as fusion tag. Reviewed

    Nakagawa C, Nishimura S, Senda-Murata K, Sugimoto K.

    Biosci Biotechnol Biochem. 雑誌   76 ( 2 )   388 - 90   2012.02

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    Kind of work:Joint Work  

  • Interaction of a goose-type lysozyme with chitin oligosaccharides as determined by NMR spectroscopy. Reviewed

    Shinya S, Ohnuma T, Kawamura S, Torikata T, Nishimura S, Katoh E, Fukamizo T.

    J Biochem. 雑誌   150 ( 5 )   569 - 77   2011.11

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    Kind of work:Joint Work  

  • Backbone chemical shifts assignments, secondary structure, and ligand binding of a family GH-19 chitinase from moss, Bryum coronatum.

    Shinya S, Nagata T, Ohnuma T, Taira T, Nishimura S, Fukamizo T.

    Biomol NMR Assign 雑誌   2011.11

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    Kind of work:Joint Work  

  • Improvement of a Venus-based bimolecular fluorescence complementation assay to visualize bFos-bJun interaction in living cells. Reviewed

    Nakagawa C, Inahata K, Nishimura S, Sugimoto K.

    Biosci Biotechnol Biochem. 雑誌   75 ( 7 )   1399 - 401   2011.07

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    Kind of work:Joint Work  

  • Kinetic and crystallographic analyses of the catalytic domain of chitinase from Pyrococcus furiosus- the role of conserved residues in the active site Reviewed

    Tsuji H, Nishimura S, Inui T, Kado Y, Ishikawa K, Nakamura T, Uegaki K.

    FEBS Journal 雑誌   277 ( 12 )   2683 - 95   2010.06

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    Kind of work:Joint Work  

  • Structural analysis of lipocalin-type prostaglandin D synthase complexed with biliverdin by small-angle X-ray scattering and multi-dimensional NMR. Reviewed

    Miyamoto Y, Nishimura S, Inoue K, Shimamoto S, Yoshida T, Fukuhara A, Yamada M, Urade Y, Yagi N, Ohkubo T, Inui T.

    J. Struct. Biol. 雑誌   169 ( 2 )   209 - 18   2010.02

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    Kind of work:Joint Work  

  • Kinetically trapped metastable intermediate of a disulfide-deficient mutant of the starch-binding domain of glucoamylase. Reviewed

    Sugimoto H, Nakaura M, Nishimura S, Karita S, Miyake H, Tanaka A.

    Protein Sci. 雑誌   18 ( 8 )   1715 - 23   2009.08

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    Kind of work:Joint Work  

  • Thermal unfolding mechanism of lipocalin-type prostaglandin D synthase Reviewed

    Tsukimi Iida, Shigenori Nishimura, Maki Mochizuki, Susumu Uchiyama, Tadayasu Ohkubo, Yoshihiro Urade, Akiyoshi Tanaka, Takashi Inui

    FEBS Journal 雑誌   275 ( 2 )   233 - 241   2007.12

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    Kind of work:Joint Work  

  • Conformational analysis of human calcitonin in solution. Reviewed

    Kiyoshi Ogawa, Shigenori Nishimura, Masamitsu Doi, H Takashima, Y Nishi, Takuya Yoshida, Tadayasu Ohkubo, Yuji Kobayashi

    J. Pept. Sci. 雑誌   12 ( 1 )   51 - 57   2006.01

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    Kind of work:Joint Work  

  • Two Additional Carbohydrate-Binding Sites of β-Amylase from Bacillus cereus var. mycoides Are Involved in Hydrolysis and Raw Starch-Binding Reviewed

    Zhengmao Ye, Hideo Miyake, Maki Tatsumi, Shigenori Nishimura, Yasunori Nitta

    J. Biochem. 雑誌   135 ( 3 )   355 - 363   2004.03

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    Kind of work:Joint Work  

  • Crystal Structure of a Catalytic Site Mutant of β-Amylase from Bacillus cereus var. mycoides Cocrystallized with Maltopentaose Reviewed

    Hideo Miyake, Genji Kurisu, Masami Kusunoki, Shigenori Nishimura, Shinichi Kitamura, Yasunori Nitta

    Biochemistry 雑誌   42 ( 19 )   5574 - 5581   2003.05

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    Kind of work:Joint Work  

  • Catalytic Mechanism of β-Amylase from Bacillus cereus var. mycoides: Chemical Rescue of Hydrolytic Activity for a Catalytic Site Mutant(Glu367- Reviewed

    Hideo Miyake, Chikako Otsuka, Shigenori Nishimura, Yasunori Nitta

    J. Biochem. 雑誌   131 ( 4 )   587 - 591   2002.04

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    Kind of work:Joint Work  

  • Conformation analysis of eel calcitonin--comparison with the conformation of elcatonin.

    Ogawa K, Nishimura S, Uchiyama S, Kobayashi K, Kyogoku Y, Hayashi M, Kobayashi Y.

    Eur. J. Biochem. 雑誌   257 ( 2 )   1998.10

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    Kind of work:Joint Work  

  • Promotion of polypeptide folding by interactions with Asn-Glycans.

    Kimura N, Uchida M, Nishimura S, Yamaguchi H.

    J. Biochem 雑誌   124 ( 4 )   1998.10

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    Kind of work:Joint Work  

  • Oxidative refolding of bovine pancreatic RNases A and B promoted by Asn-glycans Reviewed

    Nishimura I, Uchida M, Inohana Y, Setoh K, Daba K, Nishimura S, Yamaguchi H.

    J. Biochem. 雑誌   123 ( 3 )   1998.03

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    Kind of work:Joint Work  

  • Maltal binding mechanism and a role of the mobile loop of soybean beta-amylase. Reviewed

    Kunikata T, Nishimura S, Nitta Y.

    Biosci Biotechnol Biochem. 雑誌   60 ( 7 )   1996.07

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    Kind of work:Joint Work  

  • Conformational analysis of elcationin in solution Reviewed

    Ogawa K, Nishimura S, Doi M, Kyogoku Y, Hayashi M, Kobayashi Y.

    Eur. J. Biochem. 雑誌   222 ( 2 )   1994.06

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    Kind of work:Joint Work  

  • Three-dimensional structure of human insulin-like growth factor-I (IGF-I) determined by H-NMR and distance geometry Reviewed

    Sato A, Nishimura S, Ohkubo T, Kyogoku Y, Koyama S, Kobayashi M, Yasuda T, Kobayashi Y

    Int. J. Peptide Protein Res. 雑誌   41 ( 5 )   1993.05

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    Kind of work:Joint Work  

  • H-NMR Assingment and Secondary Structure of Human Insulin-Like Growth Factor-I (IGF-I) in Solution Reviewed

    Sato A, Nishimura S, Ohkubo T, Kyogoku Y, Koyama S, Kobayashi M, Yasuda T, Kobayashi Y

    J. Biochem. 雑誌   111 ( 4 )   1992.04

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    Kind of work:Joint Work  

  • Solution conformation of endothelin determined by means of H-NMR spectroscopy and distance geometry calculations Reviewed

    Haruhiko Tamaoki, Yuji Kobayashi, Shigenori Nishimura, Tadayasu Ohkubo, Yoshimasa Kyogoku, Kiichiro Nakajima, Shin-ichiro Kumagaye, Terutoshi Kimura, Shumpei Sakakibara

    Protein Engineering 雑誌   4 ( 5 )   1991.06

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    Kind of work:Joint Work  

  • A new alpha-helical motif in membrane active peptides.

    Kobayashi Y1, Sato A, Takashima H, Tamaoki H, Nishimura S, Kyogoku Y, Ikenaka K, Kondo T, Mikoshiba K, Hojo H, Aimoto S, Moroder L.

    Neurochemistry International 雑誌   18 ( 4 )   1991.03

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    Kind of work:Joint Work  

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Presentations

  • In silicoスクリーニングによるTrypanosoma brucei由来GMP reductaseに対する新規アロステリック阻害剤の開発 Domestic conference

    松本 彩佳, 竹内 悠真, 西村 重徳, 岡田 哲也, 乾 隆

    第26回 日本蛋白質科学会年会  2023.07  日本蛋白質科学会

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    Presentation type:Oral presentation (general)  

    Venue:名古屋  

  • X線結晶構造解析を利用したアデニンヌクレオチドおよびリバビリン誘導体によるTrypanosoma brucei由来GMP reductaseの阻害メカニズムの解明 Domestic conference

    竹内 悠真,馬瀬 ひかる,西村 重徳,岡田 哲也,乾 隆

    バイオ・メディカル・フォーラム2023  2023.01 

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    Presentation type:Poster presentation  

  • リポカリン型プロスタグランジンD合成酵素の多量体化による腫瘍標的ドラッグデリバリーシステムの開発

    若林 光哉, 吉田 はるな, 下地 真広, 大久保 理奈, 中辻 匡俊, 西村 重徳, 乾 隆

    日本生化学会大会プログラム・講演要旨集  2022.11  (公社)日本生化学会

  • Trypanosoma brucei由来GMP reductaseとAMPとの複合体のX線結晶構造解析 Domestic conference

    竹内 悠真, 今村 章, 岡田 哲也, 馬瀬 ひかる, 西村 重徳, 乾 隆

    第94回日本生化学会大会  2021.11 

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    Presentation type:Poster presentation  

  • Trypanosoma brucei由来GMP reductaseとAMPとの複合体のX線結晶構造解析

    竹内 悠真, 今村 章, 岡田 哲也, 馬瀬 ひかる, 西村 重徳, 乾 隆

    日本生化学会大会プログラム・講演要旨集  2021.11  (公社)日本生化学会

  • リポカリン型プロスタグランジンD合成酵素の多量体化による腫瘍標的ナノキャリアの開発

    吉田 はるな, 下地 真広, 古田 航祐, 大久保 理奈, 西村 重徳, 乾 隆

    日本生化学会大会プログラム・講演要旨集  2020.09  (公社)日本生化学会

  • リポカリン型プロスタグランジンD合成酵素の多量体化による腫瘍組織特異的ナノキャリアの開発

    吉田 はるな, 下地 真広, 古田 航祐, 大久保 理奈, 田代 朝弓, 中辻 匡俊, 西村 重徳, 乾 隆

    日本DDS学会学術集会プログラム予稿集  2020.08  日本DDS学会

  • Crystal structure of dog allergen Can f 1 Domestic conference

    Keisuke Suda, Haruka Muroya, Yuma Fukutomi, Shigenori Nishimura, Osamu Ishibashi, Takashi Inui

    第20回日本蛋白質科学会年会  2020.07 

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    Presentation type:Poster presentation  

  • 微生物由来耐熱性β-アミラーゼの性質とX線結晶構造解析

    田中 真奈, 西村 重徳, 谷 修治, 炭谷 順一, 川口 剛司

    日本生物工学会大会講演要旨集  2019.08  (公社)日本生物工学会

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Outline of collaborative research (seeds)

  • 有用酵素の構造と機能の分子レベルでの解析

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    糖質関連酵素をはじめとする様々な酵素について、X線結晶構造解析、NMR、CDを用いて構造を解析するとともに、蛋白質工学的手法を用いて触媒メカニズムを分子レベルで研究する

Grant-in-Aid for Scientific Research

  • リバビリンによる抗原虫作用の機序解明に基づくアフリカトリパノソーマ症の新薬開発

    Grant-in-Aid for Challenging Research (Pioneering)/(Exploratory)  2023

  • リバビリンによる抗原虫作用の機序解明に基づくアフリカトリパノソーマ症の新薬開発

    Grant-in-Aid for Challenging Research (Pioneering)/(Exploratory)  2022

  • リバビリンによる抗原虫作用の機序解明に基づくアフリカトリパノソーマ症の新薬開発

    Grant-in-Aid for Challenging Research (Pioneering)/(Exploratory)  2021.04

  • 低アレルゲン化ワクチンを用いたアレルゲン免疫療法によるイヌアレルギー治療への挑戦

    2017

  • マルトトリオシル配糖体合成酵素における糖転移反応の分子基盤と応用展開

    2017

  • エキソ型α-アミラーゼの基質認識と糖転移反応の分子基盤

    2014

  • GMP reductaseを標的とした新規アフリカ睡眠病治療薬の開発

    2013

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Charge of on-campus class subject

  • 生物物理化学実験

    2023   Weekly class   Undergraduate

  • 初年次ゼミナール

    2023   Weekly class   Undergraduate

  • 生命機能化学概論

    2023   Weekly class   Undergraduate

  • 生命機能化学研究プレゼンテーション

    2023   Intensive lecture   Graduate school

  • バイオインフォマティクス基礎実習

    2023   Weekly class   Undergraduate

  • 酵素化学

    2023   Weekly class   Undergraduate

  • 生物物理化学実験

    2023   Weekly class   Undergraduate

  • バイオインフォマティクス特論

    2023     Graduate school

  • 生命機能化学概論

    2022   Weekly class   Undergraduate

  • 生命機能化学研究プレゼンテーション

    2022   Intensive lecture   Graduate school

  • 生物物理化学実験

    2022   Weekly class   Undergraduate

  • 酵素化学

    2022   Weekly class   Undergraduate

  • 食品衛生学実習

    2022   Practical Training   Undergraduate

  • Laboratory Chemistry

    2021   Practical Training  

  • Enzyme Chemistry

    2021    

  • General Principles of Applied Biological Chemistry

    2021    

  • Laboratory : Biophysical Chemistry

    2021   Practical Training  

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Social Activities

  • 授業体験WEEK(高校生対象) 酵素化学

    2019.04 - 2020.03

  • 授業体験WEEK(高校生対象) 酵素化学

    2018.04 - 2019.03

  • 授業体験WEEK(高校生対象) 酵素化学

    2017.04 - 2018.03