Updated on 2024/04/08

写真a

 
Yamaji Ryoichi
 
Organization
Graduate School of Agriculture Department of Applied Biological Chemistry Professor
School of Agriculture Department of Applied Biological Chemistry
Title
Professor
Affiliation
Institute of Agriculture
Contact information
メールアドレス
Affiliation campus
Nakamozu Campus

Position

  • Graduate School of Agriculture Department of Applied Biological Chemistry 

    Professor  2022.04 - Now

  • School of Agriculture Department of Applied Biological Chemistry 

    Professor  2022.04 - Now

  • Organization for Research Promotion 生物資源開発センター 

    2022.04 - Now

Degree

  • 博士(農学) ( Others )

Research Areas

  • Life Science / Cell biology  / cell biology

  • Life Science / Food sciences  / food science (nutrition chemistry)

  • Life Science / Food sciences

Research Interests

  • primary fatty acid amide

  • obesity

  • muscle atrophy

  • muscle hypertrophy

  • isoflavone

  • vitamin A

  • flavonoid

  • carotenoid

  • deubiquitination

  • skeletal muscle

  • estrogen

  • androgen

  • hypoxia

  • sex hormone

  • transcriptional factor

  • nuclear receptor

  • phytoestrogen

  • polyphenol

  • functional food

  • 細胞生物学

  • 生化学

  • 栄養化学

  • Molecular Nutrition

  • Nutrition Chemistry

  • Cell Biology

  • Biochemistry

Research subject summary

  • 骨格筋形成(増殖、分化、肥大・萎縮)の分子機構の解明

  • 食品成分(栄養素:ビタミン、ミネラル;非栄養素:フラボノイド、脂肪酸誘導体など)による骨格筋の健康(筋量・筋力)に及ぼす効果とその分子機構の解明

  • 食品成分(栄養素・非栄養素)による抗肥満効果の分子機構の解明

  • アンドロゲン様活性物質/抗アンドロゲン様活性物質の探索と機能性食品(または化粧品、医薬品)への応用

  • 低酸素応答に関連する因子の機能解析

  • エストロゲン様活性物質/抗エストロゲン様活性物質の探索と機能性食品(または化粧品、医薬品)への応用

Research Career

  • 骨格筋量(増殖、分化、肥大)調節因子の同定とその作用機構

    骨格筋、タンパク質合成、タンパク質分解、分化促進、肥大促進  Individual

    2004.04 - Now 

  • 食品成分(生体成分)による骨格筋の肥大促進効果または萎縮抑制効果

    骨格筋、機能性食品、筋肥大、筋萎縮 

    2003.04 - Now 

  • Molecular mechanisms of sex steroid hormone signaling

    Individual

    2003.04 - Now 

  • Isolation and identification of agonists and antagonists for androgen receptor and their application to functional foods

    androgen, functional food  Individual

    2000.04 - Now 

  • Isolation and identification of agonists and antagonists for estrogen receptors and their application to functional foods

    estrogen, functional food, isoflavone  Individual

    2000.04 - Now 

  • Suppression mechanisms of hypoxia-inducible factor-1alpha

    hypoxia-inducible factor-1alpha  Individual

    1996.04 - Now 

Professional Memberships

  • 日本フードファクター学会

    2019.12 - Now   Domestic

  • The Molecular Biology Society of Japan

    2014.10 - Now   Domestic

  • The Vitamin Society of Japan

    2004.01 - Now   Domestic

  • Japanese Society of Nutrition and Food Science

    1994.01 - Now   Domestic

  • Japanese Biochemical Society

    1992.06 - Now   Domestic

  • Japan Society for Bioscience, Biotechnology, and Agrochemistry

    1986.01 - Now   Domestic

▼display all

Awards

Papers

  • Mogrol stimulates G-protein-coupled bile acid receptor 1 (GPBAR1/TGR5) and insulin secretion from pancreatic β-cells and alleviates hyperglycemia in mice

    Tanaka C.

    Scientific Reports   14 ( 1 )   3244   2024.12

  • Mogrol stimulates G-protein-coupled bile acid receptor 1 (GPBAR1/TGR5) and insulin secretion from pancreatic β-cells and alleviates hyperglycemia in mice Reviewed

    Chisato Tanaka, Naoki Harada, Yoshiaki Teraoka, Hiroki Urushizaki, Yoh Shinmori, Teruaki Onishi, Yusuke Yotsumoto, Yuta Ito, Tomoya Kitakaze, Takashi Inui, Yuji Murata, Hiroshi Inui & Ryoichi Yamaji

    Scientific Reports   14   3244   2024.02

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    Authorship:Last author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

  • All-Trans Retinoic Acid-Responsive LGR6 Is Transiently Expressed during Myogenic Differentiation and Is Required for Myoblast Differentiation and Fusion Reviewed

    24 ( 10 )   9035   2023.05( ISSN:16616596

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    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    DOI: 10.3390/ijms24109035

    PubMed

  • Mechanism of ovarian aging and its protection by food ingredients Reviewed

    Tanaka Chisato, Kitakaze Tomoya, Harada Naoki, Yamaji Ryoichi

    VITAMINS   97 ( 2 )   79 - 83   2023.02( ISSN:0006386X

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  • Transport Form and Pathway from the Intestine to the Peripheral Tissues and the Intestinal Absorption and Metabolism Properties of Oleamide Reviewed

    Yasuyuki Kobayashi, Natsumi Watanabe, Reina Hiura, Mai Kubota, Kousuke Furuta, Keiichiro Sugimoto, Kaeko Murota, Eri Nakamura, Toshiki Matsuura, Kenji Kai, Takashi Inui, Tomoya Kitakaze, Naoki Harada, Ryoichi Yamaji

    Journal of Agricultural and Food Chemistry   70 ( 49 )   15499 - 15508   2022.12( ISSN:0021-8561

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    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    This study aimed to obtain information on the transport form and pathway from the intestine to the peripheral tissues and on the intestinal absorption and metabolism properties of oleamide (cis-9-octadecenamide). Oleamide was primarily transported via the portal vein. Density gradient centrifugation indicated that plasma oleamide was enriched in the fractions containing albumin in the portal and peripheral blood. Oleamide formed a complex with albumin in an endothermic reaction (apparent Kd = 4.4 μM). The CD36 inhibitor inhibited the oleamide uptake into the intestinal epithelial Caco-2 cells, and oleamide decreased the cell surface CD36 level. The fatty acid amide hydrolase (FAAH) inhibitor increased the transepithelial transport of oleamide across Caco-2 cells and the plasma oleamide concentration in mice intragastrically administered with oleamide. These results indicate that oleamide is transported primarily via the portal vein as a complex with albumin. Furthermore, we suggest that oleamide is taken up via CD36 in the small intestine and degraded intracellularly by FAAH.

    DOI: 10.1021/acs.jafc.2c06791

    PubMed

  • Identification of G protein-coupled receptor 55 (GPR55) as a target of curcumin Reviewed

    Naoki Harada, Mai Okuyama, Yoshiaki Teraoka, Yumi Arahori, Yoh Shinmori, Hiroko Horiuchi, Paula B. Luis, Akil I. Joseph, Tomoya Kitakaze, Shigenobu Matsumura, Tohru Hira, Norio Yamamoto, Takashi Iuni, Naoki Goshima, Claus Schneider, Hiroshi Inui, Ryoichi Yamaji

    npj Science of Food   6 ( 1 )   2022.12

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    Authorship:Last author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Abstract

    The identification of molecular targets of bioactive food components is important to understand the mechanistic aspect of their physiological functions. Here, we have developed a screening system that enables us to determine the activation of G protein-coupled receptors (GPCRs) by food components and have identified GPR55 as a target for curcumin. Curcumin activated GPR55 and induced serum-response element- and serum-response factor-mediated transcription, which were inhibited by Rho kinase and GPR55 antagonists. Both the methoxy group and the heptadienone moiety of curcumin were required for GPR55 activation. The F190<sup>5.47</sup> residue of GPR55 was important for the interaction with curcumin. The curcumin-induced secretion of glucagon-like peptide-1 in GLUTag cells was inhibited by a GPR55 antagonist. These results indicate that expression screening is a useful system to identify GPCRs as targets of food components and strongly suggest that curcumin activates GPR55 as an agonist, which is involved in the physiological function of curcumin.

    DOI: 10.1038/s41538-021-00119-x

    Other URL: https://www.nature.com/articles/s41538-021-00119-x

  • Androgen receptor suppresses β-adrenoceptor-mediated CREB activation and thermogenesis in brown adipose tissue of male mice Reviewed

    Naoki Harada, Keitaro Kubo, Teruaki Onishi, Tomoya Kitakaze, Tsuyoshi Goto, Hiroshi Inui, Ryoichi Yamaji

    Journal of Biological Chemistry   298 ( 12 )   102619 - 102619   2022.12( ISSN:0021-9258

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    Authorship:Last author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Thermoregulation is a process by which core body temperature is maintained in mammals. Males typically have a lower body temperature than females. However, the effects of androgens, sex hormones that show higher levels in males, on adrenergic receptor-mediated thermogenesis remains unclear. Here, we demonstrate that androgen-androgen receptor (AR) signaling suppresses the β-adrenergic agonist-induced rise of core body temperature using castrated and AR knockout (ARKO) male mice. Furthermore, in vitro mechanistic studies show that activated AR inhibits cAMP response element (CRE)-mediated transcription by suppressing cAMP response element-binding protein (CREB) phosphorylation. The elevation of body temperature induced by the β-adrenergic agonist CL316243 was higher in ARKO and castrated mice than in control mice. Similarly, CL316243 induced a greater increase in Uncoupling protein 1 (Ucp1) expression and CREB phosphorylation in the brown adipose tissue (BAT) of ARKO mice than in that of controls. We determined that activation of AR by dihydrotestosterone suppressed β3-agonist- or forskolin-induced CRE-mediated transcription, which was prevented by AR antagonist bicalutamide. AR activation also suppressed CREB phosphorylation induced by forskolin. Moreover, we found AR nuclear localization, but not transcriptional activity, was necessary for the suppression of CRE-mediated transcription. Finally, modified mammalian two-hybrid and immunoprecipitation analyses suggest nuclear AR and CREB form a protein complex both in the presence and absence of dihydrotestosterone and forskolin. These results suggest androgen-AR signaling suppresses β-adrenoceptor-induced UCP1-mediated BAT thermogenesis by suppressing CREB phosphorylation, presumably owing to a protein complex with AR and CREB. This mechanism explains sex differences in body temperature, at least partially.

    DOI: 10.1016/j.jbc.2022.102619

    PubMed

  • Functions of Nutrient-Sensing Nuclear Receptors in Health Reviewed

    YAMAJI Ryoichi

    Journal of Nutritional Science and Vitaminology   68 ( Supplement )   S14 - S16   2022.11( ISSN:03014800

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    Authorship:Lead author, Corresponding author   International / domestic magazine:International journal  

    <p>Nutrients play important roles in the regulation of physiological and pathophysiological events in higher animals. Nuclear receptors (NRs) share a common modular functional structure and constitute a transcription factor superfamily consisting of 48 members in humans. Some NRs are activated by the binding of small lipophilic molecules such as food components including fat-soluble vitamins (vitamins A, K, and D) or lipids (phosphatidylcholine, oleoylethanolamide, or fatty acids). NRs contribute to cell growth, differentiation, or metabolic regulation. Generally, NRs bound to their ligands function as a transcription factors targeting specific DNA sequences in genes. Additionally, ligand-bound NRs mediate the activation of specific intracellular signal transduction pathways. On the other hand, some NRs are functional without binding a ligand. Information on the roles and functions of nutrient-sensing NRs in physiological or pathophysiological events not only leads to an understanding of the need for nutrients, but also contributes to the prevention and amelioration of nutrition-related diseases.</p>

    DOI: 10.3177/jnsv.68.s14

    PubMed

  • Dietary oleamide attenuates obesity induced by housing mice in small cages.

    Yasuyuki Kobayashi, Mai Kubota, Keiichiro Sugimoto, Tomoya Kitakaze, Naoki Harada, Ryoichi Yamaji

    Bioscience, Biotechnology, and Biochemistry   2022.05

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    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Physical inactivity due to prolonged sedentary behavior induces obesity. Therefore, we investigated whether housing mice in small cages to mimic sedentary behavior induced obesity and whether dietary oleamide (cis-9,10-octadeceneamide) suppressed the induced obesity. A single oral administration of oleamide (50 mg/kg) to mice resulted in the accumulation of the exogenous oleamide in abdominal visceral fat. Next, mice were housed in small cages and oleamide (50 mg/kg/d) was orally administered for 12 weeks. Housing mice in small cages impaired glucose tolerance and increased food efficiency. It also increased body weight and abdominal fat mass. Dietary oleamide improved the impairment and inhibited their increases in mice housed in small cages. Furthermore, dietary oleamide suppressed the mRNA expression of inflammation-related factors in abdominal fat of mice housed in small cages. Hence, these results indicate that although housing mice in small cages induces obesity and increases abdominal fat mass, dietary oleamide suppresses the obesity.

    DOI: 10.1093/bbb/zbac082

    PubMed

  • Curcumin activates G protein-coupled receptor 97 (GPR97) in a manner different from glucocorticoid

    Naoki Harada, Yumi Arahori, Mai Okuyama, Paula B. Luis, Akil I. Joseph, Tomoya Kitakaze, Naoki Goshima, Claus Schneider, Hiroshi Inui, Ryoichi Yamaji

    Biochemical and Biophysical Research Communications   595   41 - 46   2022.03( ISSN:0006-291X

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    Authorship:Last author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    DOI: 10.1016/j.bbrc.2022.01.075

  • Carotenoid transporter CD36 expression depends on hypoxia-inducible factor-1α in mouse soleus muscles

    Tomoya Kitakaze, Takashi Sugihira, Hiromichi Kameyama, Asami Maruchi, Yasuyuki Kobayashi, Naoki Harada, Ryoichi Yamaji

    Journal of Clinical Biochemistry and Nutrition   71 ( 2 )   112 - 121   2022( ISSN:0912-0009

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    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    DOI: 10.3164/jcbn.21-163

  • Oleamide rescues tibialis anterior muscle atrophy of mice housed in small cages.

    Kobayashi Y, Watanabe N, Kitakaze T, Sugimoto K, Izawa T, Kai K, Harada N, Yamaji R.

    Br. J. Nutr.   126 ( 4 )   2021.08

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    Authorship:Last author, Corresponding author   Kind of work:Joint Work   International / domestic magazine:International journal  

  • マウスの非アルコール性脂肪性肝炎に対する低用量エタノールの作用

    高橋 芳久, 渡部 朱織, 富樫 有紗, 渡邊 雅人, ドウングバット・エルデンソト, 小林 恭之, 原田 直樹, 山地 亮一, 杉本 圭一郎, 福里 利夫

    日本病理学会会誌   110 ( 1 )   314 - 315   2021.03( ISSN:0300-9181

  • Effects of Caffeine and Chlorogenic Acid on Nonalcoholic Steatohepatitis in Mice Induced by Choline-Deficient, L-Amino Acid-Defined, High-Fat Diet

    Erdenetsogt Dungubat, Shiori Watabe, Arisa Togashi-Kumagai, Masato Watanabe, Yasuyuki Kobayashi, Naoki Harada, Ryoichi Yamaji, Toshio Fukusato, Galtsog Lodon, Badamjav Sevjid, Yoshihisa Takahashi

    Nutrients   12 ( 12 )   3886 - 3886   2020.12

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    Several recent experimental studies have investigated the effects of caffeine and chlorogenic acid (CGA), representative ingredients of coffee, on nonalcoholic fatty liver disease (NAFLD)/nonalcoholic steatohepatitis (NASH). However, the results are conflicting, and their effects are yet to be clarified. In the present study, we examined the effects of caffeine and CGA on choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD)-fed mice, relatively new model mice of NASH. Seven-week-old male C57BL/6J mice were divided into the following groups: Control diet (control), CDAHFD (CDAHFD), CDAHFD supplemented with 0.05% (w/w) caffeine (caffeine), and CDAHFD supplemented with 0.1% (w/w) CGA (CGA). After seven weeks, the mice were killed and serum biochemical, histopathological, and molecular analyses were performed. Serum alanine aminotransferase (ALT) levels were significantly higher in the caffeine and CGA groups than in the CDAHFD group. On image analysis, the prevalence of Oil red O-positive areas (reflecting steatosis) was significantly higher in the caffeine group than in the CDAHFD group, and that of CD45R-positive areas (reflecting lymphocytic infiltration) in the hepatic lobule was significantly higher in the caffeine and CGA groups than in the CDAHFD group. Hepatic expression of interleukin (IL)-6 mRNA was higher in the caffeine and CGA groups than in the CDAHFD group, and the difference was statistically significant for the caffeine group. In conclusion, in the present study, caffeine and CGA significantly worsened the markers of liver cell injury, inflammation, and/or steatosis in NASH lesions in mice.

    DOI: 10.3390/nu12123886

  • Aronia juice supplementation inhibits lipid accumulation in both normal and obesity model mice

    Takuya Yamane, Momoko Imai, Satoshi Handa, Naoki Harada, Ryoichi Yamaji, Tatsuji Sakamoto, Tetsuo Ishida, Hiroshi Inui, Takenori Nakagaki, Yoshihisa Nakano

    PharmaNutrition   14   2020.12

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    © 2020 Elsevier B.V. Background: Obesity is a serious risk factor in health, and it is helpful for dietary supplements to prevent it. Aronia berries have various beneficial effects on health including amelioration of type 2 diabetes, obesity and hypertension. This study examined mechanistically the effects of aronia juice on the white adipose tissues in normal and obesity model mice. Methods: C57BL/6 J mice and KKAy mice were fed on a normal diet for 28 days. During this feeding period, the study group was administered with aronia juice via a water bottle, while the control group was administered with tap water. Furthermore, gene expression and mass spectrometric analyses were performed. Results: In both the mouse strains, the size of adipose cells in the study group was significantly smaller than that in the control group. In the study group, expression in the white adipose tissues of Arrdc3 mRNA was suppressed, whereas that of Sirt2 and Ucp1 mRNA was enhanced. In 3T3-L1 cells, non-cytotoxic levels of aronia juice inhibited the lipid accumulation and Arrdc3 mRNA expression. Using reverse-phase liquid chromatography, compounds suppressing Arrdc3 expression in 3T3-L1 cells were isolated from aronia juice. Mass spectrometric analysis suggested that these compounds are vicenin-2 and quercetin-diglycoside. Conclusion: The results suggest that aronia juice inhibits lipid accumulation in white adipose tissues by suppressing Arrdc3 mRNA expression in adipocytes.

    DOI: 10.1016/j.phanu.2020.100223

  • Relationship between gut environment, feces-to-food ratio, and androgen deficiency-induced metabolic disorders

    Naoki Harada, Yukari Minami, Kazuki Hanada, Ryo Hanaoka, Yasuyuki Kobayashi, Takeshi Izawa, Takashi Sato, Shigeaki Kato, Hiroshi Inui, Ryoichi Yamaji

    Gut Microbes   12 ( 1 )   1817719 - 1817719   2020.11( ISSN:1949-0976

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1080/19490976.2020.1817719

  • Effects of low ethanol consumption on nonalcoholic steatohepatitis in mice Reviewed

    Yoshihisa Takahashi, Shiori Watabe, Arisa Togashi-Kumagai, Masato Watanabe, Erdenetsogt Dungubat, Hiroyuki Kusano, Yasuyuki Kobayashi, Naoki Harada, Ryoichi Yamaji, Keiichiro Sugimoto, Toshio Fukusato

    Alcohol   87   51 - 61   2020.09( ISSN:0741-8329

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.alcohol.2020.04.004

  • β-Cryptoxanthin Improves p62 Accumulation and Muscle Atrophy in the Soleus Muscle of Senescence-Accelerated Mouse-Prone 1 Mice. Reviewed

    Mari Noguchi, Tomoya Kitakaze, Yasuyuki Kobayashi, Katsuyuki Mukai, Naoki Harada, Ryoichi Yamaji

    Nutrients   12 ( 8 )   2020.07

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    We investigated the effects of β-cryptoxanthin on skeletal muscle atrophy in senescence-accelerated mouse-prone 1 (SAMP1) mice. For 15 weeks, SAMP1 mice were intragastrically administered vehicle or β-cryptoxanthin. At 35 weeks of age, the skeletal muscle mass in SAMP1 mice was reduced compared with that in control senescence-accelerated mouse-resistant 1 (SAMR1) mice. β-cryptoxanthin increased muscle mass with an increase in the size of muscle fibers in the soleus muscle of SAMP1 mice. The expressions of autophagy-related factors such as beclin-1, p62, LC3-I, and LC3-II were increased in the soleus muscle of SAMP1 mice; however, β-cryptoxanthin administration inhibited this increase. Unlike in SAMR1 mice, p62 was punctately distributed throughout the cytosol in the soleus muscle fibers of SAMP1 mice; however, β-cryptoxanthin inhibited this punctate distribution. The cross-sectional area of p62-positive fiber was smaller than that of p62-negative fiber, and the ratio of p62-positive fibers to p62-negative fibers was increased in SAMP1 mice. β-cryptoxanthin decreased this ratio in SAMP1 mice. Furthermore, β-cryptoxanthin decreased the autophagy-related factor expression in murine C2C12 myotube. The autophagy inhibitor bafilomycin A1, but not the proteasome inhibitor MG132, inhibited the β-cryptoxanthin-induced decrease in p62 and LC3-II expressions. These results indicate that β-cryptoxanthin inhibits the p62 accumulation in fibers and improves muscle atrophy in the soleus muscle of SAMP1 mice.

    DOI: 10.3390/nu12082180

    PubMed

  • Biological Activity of Pseudovitamin B12 on Cobalamin-Dependent Methylmalonyl-CoA Mutase and Methionine Synthase in Mammalian Cultured COS-7 Cells.

    Tomohiro Bito, Mariko Bito, Tomomi Hirooka, Naho Okamoto, Naoki Harada, Ryoichi Yamaji, Yoshihisa Nakano, Hiroshi Inui, Fumio Watanabe

    Molecules (Basel, Switzerland)   25 ( 14 )   2020.07

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Adenyl cobamide (commonly known as pseudovitamin B12) is synthesized by intestinal bacteria or ingested from edible cyanobacteria. The effect of pseudovitamin B12 on the activities of cobalamin-dependent enzymes in mammalian cells has not been studied well. This study was conducted to investigate the effects of pseudovitamin B12 on the activities of the mammalian vitamin B12-dependent enzymes methionine synthase and methylmalonyl-CoA mutase in cultured mammalian COS-7 cells to determine whether pseudovitamin B12 functions as an inhibitor or a cofactor of these enzymes. Although the hydoroxo form of pseudovitamin B12 functions as a coenzyme for methionine synthase in cultured cells, pseudovitamin B12 does not activate the translation of methionine synthase, unlike the hydroxo form of vitamin B12 does. In the second enzymatic reaction, the adenosyl form of pseudovitamin B12 did not function as a coenzyme or an inhibitor of methylmalonyl-CoA mutase. Experiments on the cellular uptake were conducted with human transcobalamin II and suggested that treatment with a substantial amount of pseudovitamin B12 might inhibit transcobalamin II-mediated absorption of a physiological trace concentration of vitamin B12 present in the medium.

    DOI: 10.3390/molecules25143268

    PubMed

  • Role of gut microbiota in sex- and diet-dependent metabolic disorders that lead to early mortality of androgen receptor-deficient male mice. Reviewed

    Harada N, Hanada K, Minami Y, Kitakaze T, Ogata Y, Tokumoto H, Sato T, Kato S, Inui H, and Yamaji R.

    Am. J. Physiol. Endocrinol. Metab.   318   E525 - E537.   2020.04

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    Kind of work:Joint Work  

  • Extracellular transglutaminase 2 induces myotube hypertrophy through G protein-coupled receptor 56. Reviewed

    Tomoya Kitakaze, Miki Yoshikawa, Yasuyuki Kobayashi, Naohiro Kimura, Naoki Goshima, Takahiro Ishikawa, Yoshiyuki Ogata, Yoko Yamashita, Hitoshi Ashida, Naoki Harada, Ryoichi Yamaji

    Biochimica et biophysica acta. Molecular cell research   1867 ( 2 )   118563 - 118563   2020.02

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Skeletal muscle secretes biologically active proteins that contribute to muscle hypertrophy in response to either exercise or dietary intake. The identification of skeletal muscle-secreted proteins that induces hypertrophy can provide critical information regarding skeletal muscle health. Dietary provitamin A, β-carotene, induces hypertrophy of the soleus muscle in mice. Here, we hypothesized that skeletal muscle produces hypertrophy-inducible secretory proteins via dietary β-carotene. Knockdown of retinoic acid receptor (RAR) γ inhibited the β-carotene-induced increase soleus muscle mass in mice. Using RNA sequencing, bioinformatic analyses, and literature searching, we predicted transglutaminase 2 (TG2) to be an all-trans retinoic acid (ATRA)-induced secretory protein in cultured C2C12 myotubes. Tg2 mRNA expression increased in ATRA- or β-carotene-stimulated myotubes and in the soleus muscle of β-carotene-treated mice. Knockdown of RARγ inhibited β-carotene-increased mRNA expression of Tg2 in the soleus muscle. ATRA increased endogenous TG2 levels in conditioned medium from myotubes. Extracellular TG2 promoted the phosphorylation of Akt, mechanistic target of rapamycin (mTOR), and ribosomal p70 S6 kinase (p70S6K), and inhibitors of mTOR, phosphatidylinositol 3-kinase, and Src (rapamycin, LY294002, and Src I1, respectively) inhibited TG2-increased phosphorylation of mTOR and p70S6K. Furthermore, extracellular TG2 promoted protein synthesis and hypertrophy in myotubes. TG2 mutant lacking transglutaminase activity exerted the same effects as wild-type TG2. Knockdown of G protein-coupled receptor 56 (GPR56) inhibited the effects of TG2 on mTOR signaling, protein synthesis, and hypertrophy. These results indicated that TG2 expression was upregulated through ATRA-mediated RARγ and that extracellular TG2 induced myotube hypertrophy by activating mTOR signaling-mediated protein synthesis through GPR56, independent of transglutaminase activity.

    DOI: 10.1016/j.bbamcr.2019.118563

    PubMed

  • Food texture affects glucose tolerance by altering pancreatic β-cell function in mice consuming high-fructose corn syrup. Reviewed

    Naoki Harada, Masayuki Nomura, Yasuhiro Yoda, Shigenobu Matsumura, Hiroshi Inui, Ryoichi Yamaji

    PloS one   15 ( 5 )   e0233797   2020

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    The incidence of metabolic diseases, such as type 2 diabetes, has increased steadily worldwide. Diet, beverages, and food texture can all markedly influence these metabolic disorders. However, the combined effects of food texture and beverages on energy metabolism remains unclear. In the present study, we examined the effect of food texture on energy metabolism in mice administered high-fructose corn syrup (HFCS). Mice were fed a soft or hard diet along with 4.2% HFCS or tap water. Body weight and total caloric intake were not affected by food texture irrespective of HFCS consumption. However, caloric intake from HFCS (i.e., drinking volume) and diet were higher and lower, respectively, in the hard food group than in the soft food group. The hard food group's preference for HFCS was absent in case of mice treated with the μ-opioid receptor antagonist naltrexone. Despite increased HFCS consumption, blood glucose levels were lower in the hard-diet group than in the soft-diet group. In HFCS-fed mice, insulin levels after glucose stimulation and insulin content in the pancreas were higher in the hard food group than the soft food group, whereas insulin tolerance did not differ between the groups. These food texture-induced differences in glucose tolerance were not observed in mice fed tap water. Thus, food texture appears to affect glucose tolerance by influencing pancreatic β-cell function in HFCS-fed mice. These data shed light on the combined effects of eating habits and food texture on human health.

    DOI: 10.1371/journal.pone.0233797

    PubMed

  • アロニア果汁含有脂肪蓄積抑制物質の効果

    山根 拓也, 今井 ももこ, 小塚 美由記, 飯田 聡史, 原田 直樹, 石田 哲夫, 竹中 重雄, 山地 亮一, 阪本 龍司, 乾 博, 山本 好男, 大久保 岩男, 中垣 剛典, 中野 長久

    日本生化学会大会プログラム・講演要旨集   92回   [3P - 254]   2019.09

  • Fetal androgen signaling defects affect β-cell mass and function, leading to glucose intolerance in high-fat diet-fed male rats. Reviewed

    Harada N, Yotsumoto Y, Katsuki T, Yoda Y, Masuda T, Nomura M, Shiraki N, Inui H, Yamaji R

    American Journal of Physiology - Endocrinology and Metabolism   317 ( 5 )   E731 - E741   2019.07( ISSN:0193-1849

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    We previously demonstrated that androgen signaling expands pancreatic β-cell mass in the sexual maturation period (Am J Physiol Endocrinol Metab 314: E274-E286, 2018). The aim of this study was to elucidate whether fetal androgen signaling plays important roles in β-cell mass development and β-cell function in adulthood, defects of which are associated with type 2 diabetes mellitus. In the pancreas of male fetuses, androgen receptor (AR) was strongly expressed in the cytoplasm and at the cell membrane of Nkx6.1-positive β-cell precursor cells but was markedly reduced in insulin-positive β-cells. Administration of the anti-androgen flutamide to pregnant dams during late gestation reduced β-cell mass and Ki67-positive proliferating β-cells at birth in a male-specific manner without affecting body weight. The decrease of β-cell mass in flutamide-exposed male rats was not recovered when rats were fed a standard diet, whereas it was fully recovered when rats were fed a high-fat diet (HFD), at 6 and 12 wk of age. Flutamide exposure in utero led to the development of glucose intolerance in male rats due to a decrease in insulin secretion when fed HFD but not standard diet. Insulin sensitivity did not differ between the two groups irrespective of diet. These results indicated that the action of fetal androgen contributed to β-cell mass expansion in a sex-specific manner at birth and to the development of glucose intolerance by decreasing the secretion of insulin in HFD-fed male rats. Our data demonstrated the involvement of fetal androgen signaling in hypothesized sex differences in the developmental origins of health and disease by affecting pancreatic β-cell function.

    DOI: 10.1152/ajpendo.00173.2019

    PubMed

  • 5-Hydroxy-7-methoxyflavone derivatives from Kaempferia parviflora induce skeletal muscle hypertrophy. Reviewed

    Shintaro Ono, Naoki Yoshida, Daisuke Maekawa, Tomoya Kitakaze, Yasuyuki Kobayashi, Takehiro Kitano, Takanori Fujita, Hirotaka Okuwa-Hayashi, Naoki Harada, Yoshihisa Nakano, Ryoichi Yamaji

    Food science & nutrition   7 ( 1 )   312 - 321   2019.01

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Skeletal muscle plays a critical role in locomotion and energy metabolism. Maintenance or enhancement of skeletal muscle mass contributes to the improvement of mobility and prevents the development of metabolic diseases. The extracts from Kaempferia parviflora rhizomes contain at least ten methoxyflavone derivatives that exhibit enhancing effects on ATP production and glucose uptake in skeletal muscle cells. In the present study, we investigated the effects of ten K. parviflora-derived methoxyflavone derivatives (six 5,7-dimethoxyflavone (DMF) derivatives and four 5-hydroxy-7-methoxyflavone (HMF) derivatives) on skeletal muscle hypertrophy. Murine C2C12 myotubes and senescence-accelerated mouse-prone 1 (SAMP1) mice treated with methoxyflavones were used as experimental models to determine the effects of HMF derivatives on myotube diameter and size and muscle mass. The four HMF derivatives, but not the six DMF derivatives, increased myotube diameter. The 5-hydroxyflavone, 7-methoxyflavone, and 5,7-dihydroxyflavone had no influence on myotube size, a result that differed from HMF. Dietary administration of the mixture composed of the four HMF derivatives resulted in increase in the soleus muscle size and mass in SAMP1 mice. HMF derivatives also promoted protein synthesis in myotubes, and treatment with the intracellular Ca2+ chelator BAPTA-AM, which depletes intracellular Ca2+ levels, inhibited this promotion. Furthermore, BAPTA-AM inhibited HMF-promoted protein synthesis even when myotubes were incubated in Ca2+-free medium. These results indicate that HMF derivatives induce myotube hypertrophy and that both the 5-hydroxyl group and the 7-methoxy group in the flavones are necessary for myotube hypertrophy. Furthermore, these results suggest that HMF-induced protein synthesis requires intracellular Ca2+, but not extracellular Ca2+.

    DOI: 10.1002/fsn3.891

    PubMed

  • ラット小腸粘膜および組換えマウス型のβ-カロテン15,15′-モノオキシゲナーゼ活性に及ぼすリノール酸の影響 Reviewed

    北川雅啓,山地亮一,原田直樹,中野長久,細谷圭助

    ビタミン 雑誌   92   416 - 423   2018.09

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  • Theophylline suppresses interleukin-6 expression by inhibiting glucocorticoid receptor signaling in pre-adipocytes. Reviewed

    Mitani T, Takaya T, Harada N, Katayama S, Yamaji R, Nakamura S, and Ashida H.

    Arch. Biochem. Biophys. 雑誌   646   98 - 106   2018.04

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  • Lactoferrin promotes murine C2C12 myoblast proliferation and differentiation, and myotube hypertrophy. Reviewed

    Kitakaze T, Oshimo M, Kobayashi Y, Ryu M, Suzuki Y.A, Inui H, Harada N, and Yamaji R.

    Mol. Med. Rep. 雑誌   17   5912 - 5920   2018.04

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  • Heat stress prevents the decrease in succinate dehydrogenase activity in the extensor digitorum longus of streptozotocin-induced diabetic rats. Reviewed

    (84) Nonaka K, Une S, Komatsu M, Yamaji R, Akiyama J.

    Physiol. Res. 雑誌   67 ( 1 )   2018.03

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  • Stereoselective effects of lactate enantiomers one the enhanecment of 3T3-L1 adipocyte differentiation. Reviewed

    Harada N, Hirano I, Inui H, and Yamaji R.

    Biochem. Biophys. Res. Commun. 雑誌   498   105 - 110   2018.03

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  • Androgen signaling expands β-cell mass in male rats and β-cell androgen receptor is degraded under high-glucose conditions. Reviewed

    (85) Harada N, Yoda Y, Yotsumoto Y, Masuda T, Takahashi Y, Katsuki K, Kai K, Shiraki N, Inui H, and Yamaji R.

    Am. J. Physiol. Endocrinol. Metab. 雑誌   314 ( 3 )   2018.03

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  • Lactoferrin induces tropoelastin expression by activating the lipoprotein receptor-related protein 1-mediated phosphatidylinositol 3-kinase/Akt pathway in human dermal fibroblasts. Reviewed

    Ryu M, Nogami A, Kitakaze T, Harada N, Suzuki A. Y, Yamaji R.

    Cell Biol. Int. 雑誌   41 ( 12 )   2017.12

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  • Daidzein down-regulates ubiquitin-specific protease 19 expression through estrogen receptor β and increases skeletal muscle mass in young female mice. Reviewed

    Ogawa M, Kitano T, Kawata N, Sugihira T, Kitakaze T, Harada N, Yamaji R.

    J. Nutr. Biochem. 雑誌   49   2017.11

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  • S-Equol activates cAMP signaling at the plasma membrane of INS-1 pancreatic β-cells and protects against streptozotocin-induced hyperglycemia by increasing β-cell function in male mice Reviewed

    (81) Horiuchi H, Usami A, Shirai R, Harada N, Ikushiro S, Sakaki T, Nakano Y, Inui H, and Yamaji R.

    J. Nutr.   147 ( 9 )   2017.09

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  • Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) aggregation causes mitochondrial dysfunction during oxidative stress-induced cell death. Reviewed

    Itakura M, Nakajima H, Kubo T, Kaneshige A, Harada N, Izawa T, Azuma YT, Kuwamura M, Yamaji R, and Takeuchi T.

    J. Biol. Chem. 雑誌   292   2017.03

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  • Hypogonadism alters cecal and fecal microbeta in male mice. Reviewed

    Harada N, Hanaoka R, Hanada K, Izawa T, Inui H, Yamaji R.

    Gut Microbes 雑誌   7   533 - 539   2016.11

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  • The collagen derived dipeptide hydroxyprolyl-glycine promotes C2C12 myoblast differentiation and myotube hypertrophy. Reviewed

    Kitakaze T, Sakamotoa T, Kitano T, Inoue N, Sugihara F, Harada N, Yamaji R.

    Biochem. Biophys. Res. Commun. 雑誌   478   1292 - 1297   2016.09

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  • Mogrol Derived from Siraitia grosvenorii Mogrosides Suppresses 3T3-L1 Adipocyte Differentiation by Reducing cAMP-Response Element-Binding Protein Phosphorylation and Increasing AMP-Activated Protein Kinase Phosphorylation. Reviewed

    Harada N, Ishihara M, Horiuchi H, Ito Y, Tabata H, Suzuki Y.A, Nakano Y, Yamaji R, Inui H.

    PLoS One 雑誌   11   2016.09

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  • pVHL-mediated degradation of HIF-2a regulates estrogen receptor a expression in normoxic breast cancer cells. Reviewed

    Higashimura Y, Kitakaze T, Harada N, Inui H, Nakano Y, Yamaji R.

    FEBS Lett. 雑誌   590   2690 - 2699   2016.08

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  • Castration influences intestinal microflora and induces abdominal obesity in high-fat diet-fed mice. Reviewed

    Harada N, Hanaoka R, Horiuchi H, Kitakaze T, Mitani T, Inui H, Yamaji R.

    Sci. Rep. 雑誌   6   2016.03

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  • Glyceraldehyde-3-Phosphate Dehydrogenase Aggregates Accelerate Amyloid-β Amyloidogenesis in Alzheimer Disease. Reviewed

    Itakura M, Nakajima H, Kubo T, Semi Y, Kume S, Higashida S, Kaneshige A, Kuwamura M, Harada N, Kita A, Azuma YT, Yamaji R, Inui T, Takeuchi T.

    J. Biol. Chem. 雑誌   290   26072 - 26087   2015.10

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  • Autophagic degradeation of the androgen receptor mediated by increased phosphorylation of p62 suppresses apoptosis in hypoxia. Reviewed

    Mitani T, Minami M, Harada N, Ashida H, Yamaji R.

    Cell Signal. 雑誌   27   1994 - 2001   2015.10

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  • Construction of sensitive reporter assay yeasts for comprehensive detection of ligand activities of human corticosteroid receptors through inactivation of CWP and PDR genes. Reviewed

    Ito-Harashima S, Shiizaki K, Kawanishi M, Kakiuchi K, Onishi K, Yamaji R, Yagi T.

    J. Pharmacol. Toxicol. Methods. 雑誌   74   41 - 52   2015.06

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  • β-Carotene increases muscle mass and hypertrophy in the soleus muscle in mice. Reviewed

    Kitakaze T, Harada N, Imagita H, Yamaji R.

    J. Nutr. Sci. Vitaminol. 雑誌   61   481 - 487   2015.06

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  • 多機能性を持つ Moonlighting Proteins Reviewed

    原田直樹、三谷塁一、山地亮一

    生化学 雑誌   87   279 - 285   2015.06

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  • Androgen receptor silences thioredoxin-interacting protein and competitively inhibits glucocorticoid receptor-mediated apoptosis in pancreatic β-cells. Reviewed

    Harada N, Katsuki T, Takahashi Y, Masuda T, Setoguchi M, Adachi T, Izawa T, Kuwamura M, Nakano Y, Yamaji R, Inui H.

    J. Cell. Biochem. 雑誌   116   998 - 1006   2015.06

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  • Competitive and compensatory effects of androgen signaling and glucocorticoid signaling. Reviewed

    Harada N, Inui H, Yamaji R.

    Receptor Clin. Investig. 雑誌   2   2015.06

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  • Female-specific regulation of skeletal muscle mass by USP19 in young mice. Reviewed

    Ogawa M, Kitakaze T, Harada N, Yamaji R.

    J. Endocrinol. 雑誌   225   135 - 145   2015.06

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  • Protein arginine metyltransferase 10 is required for androgen-dependent proliferation of LNCaP prostate cancer cells. Reviewed

    Harada N, Takagi T, Nakano Y, Yamaji R, Inui H.

    Biosci. Biotechnol. Biochem. 雑誌   79   1430 - 1437   2015.04

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  • . Inhibitory effects of eucalyptus and banaba leaf extracts on nonalcoholic steatohepatitis induced by a high-fructose/high-glucose diet in rats. Reviewed

    Takahashi Y, Sugimoto K, Soejima Y, Kumagai A, Koeda T, Shojo A, Nakagawa K, Harada N, Yamaji R, Inui H, Yamanouchi T, Fukusato T.

    Biomed Res Int. 雑誌   2015   2015.04

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  • Dodecylamine derivative of hydroxocobalamin acts as a potent inhibitor of clbalamin-dependent methionine synthase in mammalian cultured COS-7 cells. Reviewed

    Bito T, Yasui M, Iwaki T, Yabuta Y, Ichiyanagi T, Yamaji R, Nakano Y, Inui H, Watanabe F.

    Food Nutr. Sci. 雑誌   5   2014.07

  • S-Equol enantioselectively activates cAMP-protein kinase A signaling and reduces alloxan-induced cell death in INS-1 pancreatic β-cells. Reviewed

    Horiuchi H, Harada N, Adachi T, Nakano Y, Inui H, Yamaji R.

    J. Nutr. Sci. Vitaminol. 雑誌   60 ( 4 )   2014.04

  • Resveratrol inhibits hypoxia-inducible factor-1α-mediated androgen receptor signaling and represses tumor progression in castration-resistant prostate cancer. Reviewed

    Mitani T, Harada N, Tanimori S, Nakano Y, Inui H, Yamaji R.

    J. Nutr. Sci. Vitaminol. 雑誌   60 ( 2 )   2014.04

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  • Resveratrol reduces the hypoxia-induced resistance to doxorubicin in breast cancer cells. Reviewed

    Mitani T, Ito Y, Harada N, Nakano Y, Inui H, Yamaji R.

    J. Nutr. Sci. Vitaminol. 雑誌   60   122 - 128   2014.02

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  • β-Carotene 15,15’-monooxygenase 1 (BCMO1) の新しい機能 Reviewed

    北風智也、原田直樹、山地亮一

    ビタミン 雑誌   87   696 - 699   2013.12

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  • Oligosaccharides from agar inhibit murine intestinal inflammation through the induction of heme oxygenase-1 expression. Reviewed

    Higashimura Y, Naito Y, Takagi T, Mizushima K, Hirai Y, Harusato A, Ohnogi, H, Yamaji R, Inui H, Nakano Y, Yoshikawa T.

    J. Gastroenterol. 雑誌   48   897 - 909   2013.08

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  • エストロゲン受容体βアゴニストとしてのS-エクオールの女性疾患に対する有効性に関する最近の知見 Reviewed

    小川真弘、原田直樹、山地亮一

    ビタミン 雑誌   87   450 - 453   2013.08

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  • Identification of carbonyl reductase 1 as a resveratrol-binding protein by affinity chromatography using 4´-amino-3,5-dihydroxy-trans-stilbene. Reviewed

    Ito Y, Mitani T, Harada N, Isayama A, Tanimori S, Takenaka S, Nakano Y, Inui H, Yamaji R.

    J. Nutr. Sci. Vitaminol. 雑誌   59   358 - 364   2013.04

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  • The preventive effect of beta-carotene on denervation-induced soleus muscle atrophy in mice. Reviewed

    Ogawa M, Kariya Y, Kitakaze T, Yamaji R, Harada N, Sakamoto T, Hosotani K, Nakano Y, Inui H.

    Br. J. Nutr. 雑誌   109   1349 - 1358   2013.04

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  • Glyceraldehyde-3-phosphate dehydrogenase regulates cyclooxygenase-2 expression by targeting mRNA stability Reviewed

    Ikeda I, Yamaji R, Irie K, Kioka N, Murakami A.

    Arch. Biochem. Biophys. 雑誌   528   141 - 147   2012.12

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  • Coordinated action of Hypoxia-Inducible Factor-1alpha and beta-Catenin in Androgen Receptor Signaling Reviewed

    Mitani T, Harada N, Nakano Y, Inui H, and Yamaji R.

    J. Biol. Chem. 雑誌   287   33594 - 33606   2012.09

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  • Androgen deprivation causes truncation of the C-terminal region of androgen receptor in human prostate cancer LNCaP cells Reviewed

    Harada N, Inoue K, Yamaji R, Nakano Y, and Inui H.

    Cancer Sci. 雑誌   103   1022 - 1027   2012.06

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  • 17beta-Estradiol represses myogenic differentiation by increasing ubiquitin-specific peptidase 19 through estrogen receptor alpa Reviewed

    Ogawa M, Yamaji R, Higashimura Y, Harada N, Ashida H, Nakano Y, and Inui H.

    J. Biol. Chem. 雑誌   286   41455 - 41465   2011.12

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  • Kelch-like 20 up-regulates the expression of hypoxia-inducible factor-2 alpha through hypoxia- and von Hippel-Lindau tumor suppressor protein-independent regulatory mechanisms Reviewed

    Higashimura Y, Terai T, Yamaji R, Mitani T, Ogawa M, Harada N, Inui H, and Nakano Y.

    Biochem. Biophys. Res. Commun. 雑誌   413   201 - 205   2011.09

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  • Up-regulation of glyceraldehyde-3-phosphate dehydrogenase gene expression by HIF-1 activity depending on Sp1 in hypoxic breast cancer cells. Reviewed

    Higashimura Y, Nakajima Y, Yamaji R (責任著者), Harada N, Shibasaki F, Nakano Y, and Inui H.

    Arch. Biochem. Biophys. 雑誌   509   2011.05

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  • (-)-Epigallocatechin-3-gallate suppresses growth of AZ521 human gastric cancer cells by targeting the DEAD box RNA helicase p68. Reviewed

    Tanaka T, Ishii T, Mizuno D, Mori T, Yamaji R, Nakamura Y, Kumazawa S, Nakayama T, and Akagawa M.

    Free Radic. Biol. Med. 雑誌   50   1324 - 1335   2011.03

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  • Suppression of Inducible Nitric Oxide Synthase Expression and Amelioration of Lipopolysaccharide-induced Liver Injury by Polyphenolic Compounds in Eucalyptus globulus Leaf Extract. Reviewed

    Sugimoto K, Sakamoto S, Nakagawa K, Hayashi S, Harada N, Yamaji R, Nakano Y, Inui H.

    Food Chemistry 雑誌   125   442 - 446   2011.03

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  • Hypoxia enhances transcriptional activity of androgen receptor through hypoxia-inducible factor-1α in a low androgen environment. Reviewed

    Mitani T, Yamaji R(責任著者), Higashimura Y, Harada N, Nakano Y, Inui H.

    J. Steroid Biochem. Mol. Biol. 雑誌   123   58 - 64   2011.01

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  • Inhibitory mechanisms of the transcriptional activity of androgen receptor by resveratrol: implication of DNA binding and acetylation of the receptor. Reviewed

    Harada N, Atarashi K, Murata Y, Yamaji R(責任著者), Nakano Y, Inui H.

    J. Steroid. Biochem. Mol. Biol. 雑誌   123   65 - 70   2011.01

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  • Construction of a dominant negative form of human hypoxia-inducible factor-2α. Reviewed

    Higashimura Y, Yamaji R(責任著者), Harada N, Nakao M, Nakao M, Nakano Y, Inui H.

    Biosci. Biotech. Biochem. 雑誌   74   2100 - 2102   2010.10

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  • A Yeast Bioassay for Androgenic and Anti-Androgenic Compounds Based on the NH2- and COOH-Terminal Interaction of Androgen Receptor. Reviewed

    Ogawa M, Yamaji R(責任著者), Mitani T, Murata Y, Nakao M, Harada N, Nakano Y, Inui H.

    Biosci. Biotech. Biochem. 雑誌   74   1965 - 1968   2010.09

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  • Sensitive and convenient yeast reporter assay for high-throughput analysis by using a secretory luciferase from Cypridina noctiluca. Reviewed

    Tochigi Y, Sato N, Sahara T, Wu C, Saito S, Irie T. Fujibuchi W, Goda T, Yamaji R, Ogawa M, Ohmiya Y, Ohgiya S.

    Anal. Chem. 雑誌   82   5768 - 5776   2010.07

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  • Methyladeninylcobamide functions as the cofactor of methionine synthase in a Cyanobacterium, Spirulina platensis NIES-39. Reviewed

    Tanioka Y, Miyamoto E, Yabuta Y, Ohnishi K, Fujita T, Yamaji R, Misono H, Shigeoka S, Nakano Y, Inui H, Watanabe F.

    FEBS Lett. 雑誌   584   3223 - 3226   2010.06

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  • Involvement of three glutamine tracts in human androgen receptor transactivation Reviewed

    Harada N., Mitani T, Higashimura Y, Yamaji R(責任著者), Okamoto K, Nakano Y, Inui H.

    J. Steroid Biochem.Mol.Biol. 雑誌   118   77 - 84   2010.01

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  • Occurrence of pseudovitamin B12 and its possible function as the cofactor of clbalamin-depdendent methionine synthase in a Cyanobactreium Reviewed

    Tanioka Y, Yabuta Y, Yamaji R, Shigeoka S, Nakano Y, Watanabe F, Inui H.

    J. Nutr. Sci. Vitaminol. 雑誌   55   518 - 521   2009.12

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  • Rats Fed Fructose-Enriched Diets Have Characteristics of Nonalcoholic Hepatic Steatosis. Reviewed

    Kawasaki, T., Igarashi, K., Koeda, T., Sugimoto, K., Nakagawa, K., Hayashi, S., Yamaji, R., Inui, H., Fukusato, T., Yamanouchi T.

    J. Nutr. 雑誌   139   2067 - 2071   2009.11

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  • Inhibitive effects of alkyl gallates on hyaluronidase and collagenase Reviewed

    Barla F, Higashijima H, Funai S, Sugimoto K, Harada N, Yamaji R, Fujita T, Nakano Y, Inui H.

    Biosci. Biotechnol. Biochem. 雑誌   73   2335 - 2337   2009.10

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  • Hydrolyzable tannins as antioxidants in the leaf extract of Eucalyptus globulus possessing tyrosinase and hyaluronidase inhibitory activities Reviewed

    Sugimoto, K., Nakagawa, K., Hayashi, S., Amakura, Y., Yoshimura, M., Yoshida, T., Yamaji, R., Nakano, Y., Inui, H.

    Food Sci. Technol. Res. 雑誌   15   331 - 336   2009.08

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  • Cobalamin deficiency results in an abnormal increase in L-methylmalonyl-CoA mutase expression in rat liver and COS-7 cells. Reviewed

    Nakao M., Hironaka S., Harada N., Adachi T., Bito T., Yabuta Y., Watanabe F., Miura T., Yamaji R., Inui H., and Nakano Y.

    Brit. J. Nutr. 雑誌   101 ( 4 )   492 - 498   2009.04

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  • Aspartyl-tRNA synthetases from two halophilic archae Halobacterium salinarum and Haloferax volcanii Reviewed

    Ihara, H., Shinoda, H., Yamaji, R., Inui, H., Miyatake, K., Wadano, A., Nakano, Y., Ishigami, M.

    Appl. Biol. Sci 雑誌   9   2008.12

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  • ARA24/Ran enhances the androgen-dependent NH2- and COOH-terminal interaction of the androgen receptor. Reviewed

    Harada N., Ohmori Y., Yamaji R.(責任著者), Higashimura Y., Okamoto K., Isohashi F., Nakano Y., and Inui H.

    Biochem. Biophys. Res. Commun. 雑誌   373 ( 3 )   373 - 377   2008.04

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  • Covalent modification of proteins by green tea polyphenol (-)-epigallocatechin-3-gallate through autoxidation. Reviewed

    Ishii T., Mori T., Tanaka T., Mizuno D., Yamaji R., Kumazuwa S., Nakayama T., and Akagawa M.

    Free Radic. Biol. Med. 雑誌   45 ( 10 )   1384 - 1394   2008.04

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  • RanBP10 acts as a novel coactivator for the androgen receptor. Reviewed

    Harada N, Yokoyama T, Yamaji R(責任著者), Nakano Y, Inui H.

    Biochem Biophys Res Commun. 雑誌   368 ( 1 )   121 - 125   2008.03

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  • Vitamin B12 deficiency results in abnormal regulation of serine dehydratase and tyrosine aminotransferase activities correlated with impairment of the adenylyl cyclase system in rat liver Reviewed

    Ebara S., Nakao M., Tomoda M., Yamaji R., Watanabe F., Inui H., Nakano Y.

    Brit. J. Nutr. 雑誌   99 ( 3 )   503 - 510   2008.03

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  • Resveratrol down-regulates the androgen receptor at the post-translational level in prostate cancer cells Reviewed

    Harada N., Murata Y., Yamaji R.(責任著者), Miura T., Inui H., Nakano Y.

    J. Nutr. Sci. Vitaminol. 雑誌   53 ( 6 )   556 - 560   2007.12

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  • Glyceraldehyde-3-phosphate dehydrogenase enhances transcriptional activity of androgen receptor in prostate cancer cells Reviewed

    Harada N., Yasunaga R., Higashimura Y., Yamaji R.(責任著者), Fujimoto K., Moss J., Inui H., Nakano Y.

    J. Biol. Chem. 雑誌   282 ( 31 )   22651 - 22661   2007.08

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  • Abnormal Increase in the Expression Level of Proliferation Cell Nucl ear Antigen (PCNA) in the Liver and Hepatic Injury in Rats with Dietary Cobala min Deficiency Reviewed

    Nakao M. Kono N. Adachi S. Ebara S. Adachi T. Miura. T. Yamaji R. Inui H and Nakano Y.

    J. Nutr. Sci. Vitaminol. 雑誌   2006.04

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  • Recent advances in molecular pharmacology of the histamine systems: organic cation transporters as a histamine transporter and histamine metabolism Reviewed

    Ogasawara M., Yamauchi K., Satoh Y., Yamaji R., Inui K., Jonker J.W., Schinkel A. H., Maeyama K.

    J. Pharmacol. Sci. 雑誌   101   24 - 30   2006.04

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  • Glyceraldehyde-3-phosphate dehydrogenase in the extracellular space inhibits cell spreading Reviewed

    Yamaji R(責任著者), Chatani E, Harada N, et al.

    BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 雑誌   1726 ( 3 )   261 - 271   2005.11

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  • Eucalyptus leaf extract inhibits intestinal fructose absorption, and suppresses adiposity due to dietary sucrose in rats Reviewed

    Sugimoto K, Suzuki J, Nakagawa K, Hayashi S, Enomoto T, Fujita T, Yamaji R, Inui H, Nakano Y

    BRITISH JOURNAL OF NUTRITION 雑誌   93 ( 6 )   957 - 963   2005.06

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  • Effect of carotenoids and ascorbic acid of Japanese persimmons on cellular lipid peroxidation in HepG2 cells Reviewed

    Hosotani K, Kawahata A, Koyama K, Murakami C, Yoshida H, Yamaji R, Inui H, Nakano Y

    BIOFACTORS 雑誌   21 ( 41643 )   241 - 245   2004.04

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  • Hypoxic up-regulation of triosephosphate isomerase expression in mouse brain capillary endothelial cells. Reviewed

    R. Yamaji (責任著者), K. Fujita, I. Nakanishi, K. Nagao, M. Naito, T. Tsuruo, H. Inui, Y. Nakano

    Arch. Biochem. Biophys. 雑誌   432 ( 2 )   332 - 342   2004.03

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  • Hypoxia-induced megaloblastosis in vitamin B12-deficient rats Reviewed

    S. Ebara, S. Adachi, S. Takenaka, T. Enomoto, F. Watanabe, R. Yamaji, H. Inui, Y. Nakano

    Brit. J. Nutr. 雑誌   89 ( 4 )   441 - 444   2003.08

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  • Purification and characterization of homodimeric methylmalonyl-CoA mutase from Sinorhizobium meliloti Reviewed

    E. Miyamoto, F. Watanabe, T. C. Charles, R. Yamaji, H. Inui, Y. Nakano

    Arch. Microbiol. 雑誌   180 ( 2 )   151 - 154   2003.08

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  • Nitric oxide synthase induction, cGMP elevation, and biopterin synthesis in vascular smooth muscle cells stimulated with interleukin-1b in hypoxia Reviewed

    K. Nagao, S. Takanaka, R. Yamaji, H. Inui, Y. Nakano

    J. Biochem. (Tokyo) 雑誌   133 ( 4 )   501 - 505   2003.04

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  • Translational regulation of histamine N-methyltransferase Reviewed

    M. Ogasawara, K. Yamauchi, R. Yamaji, K. Maeyama

    J. Pharmacol. Sci. 雑誌   91, suppl. 1   2003.04

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  • Hypoxia up-regulates glyceraldehyde-3-phosphate dehydrogenase in mouse brain capillary endothelial cells: involvement of Na+/Ca2+ exchanger Reviewed

    R. Yamaji (責任著者), K. Fujita, S. Takahashi, H. Yoneda, K. Nagao, W. Masuda, M. Naito, T. Tsuruo, K. Miyatake, H. Inui, Y. Nakano

    Biochim. Biophys. Acta 雑誌   1593 ( 41673 )   269 - 276   2003.02

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  • Occurrence of a novel NADP+-linked alcohol dehydrogenase in Euglena gracilis Reviewed

    I. Munir, M. Nakazawa, K, Harano, R. Yamaji, H. Inui, K. Miyatake, Y. Nakano

    Comp. Biochem. Physiol. B Biochem. Mol. Biol. 雑誌   132 ( 3 )   535 - 540   2002.07

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  • Purification and characterization of Methylmalonyl-CoA mutase from a methanol-utilizing bacterium, Methylobacterium extorquens NR-1 Reviewed

    E. Miyamoto, W. Watanabe, R. Yamaji, H. Inui, K. Sato, Y. Nakano

    J. Nutr. Sci. Vitaminol. 雑誌   48 ( 3 )   242 - 246   2002.06

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  • Inflammatory responses to lipopolysaccharide are suppressed in 40% energy-restricted mice Reviewed

    Matsuzaki. J(学生), Kuwamura. M, Yamaji. R, Inui. H, Nakano. Y.

    J. Nutr. 雑誌   vol.131   2139 - 2144   2001.12

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  • Implanted tumor growth is suppressed and survival is prolonged in sixty percent of food-restricted mice Reviewed

    Matsuzaki. J(学生), Yamaji. R, Kiyomiya. K, Kurebe. M, Inui. H, Nakano. Y.

    J. Nutr. 雑誌   vol.130   111 - 115   2000.12

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  • Cobalamin Deficiency results n severe metabolic disorder of serine and threonine in rats Reviewed

    Ebara. S(学生), Toyoshima. S, Matsumura. T, Adachi. S, Takenaka. S, Yamaji. R, Watanabe. F, Miyatake. K, Inui. H, Nakano. Y.

    Biochim. Biophys. Acta 雑誌   vol.1568   111 - 117   2000.12

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  • The origin of pyruvate: NADP+ oxidoreductase in mitochondria of Euglena gracilis Reviewed

    Nakazawa. M, Inui. H, Yamaji. R, Yamamoto. T, Takenaka. S, Ueda. M, Nakano. Y. Miyatake. K.

    FEBS Lett. 雑誌   vol.479   155 - 157   2000.12

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  • Increases in gastric histidine decarboxylase activity and plasma gastrin level in streptozotocin-induced type I diabetic rats Reviewed

    Inui. H, Yasuno. R, Takenoshita. M, Ohnishi. Y, Sakamoto. M, Matsuzaki. J(学生), Yamaji. R, Miyatake. K, Yamatodani. A, Nakano. Y.

    J. Nutr. Sci. Vitaminol. 雑誌   vol.46   144 - 148   2000.12

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  • Identification and localization of two brefeldin A-inhibited guanine nucleotide-exchange proteins for ADP-ribosylation factors in a macromolecular complex Reviewed

    Yamaji. R(責任著者), Adamik. R, Takeda. K, Togawa. A, Pacheco-Rodriguez. G, Ferrans. V. J, Moss. J, Vaughan. M.

    Proc. Natl. Acad. Sci. USA 雑誌   vol.97   2567 - 2572   2000.12

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  • Disordered expression of the sucrase-isomaltase complex in the small intestine in Otsuka Long-Evans Tokushima fatty rats, a model of non-insulin-dependent diabetes mellitus with insulin resistance Reviewed

    Adachi. T, Takenoshita. M, Katsura. H, Yasuda. K, Tsuda. K, Seino. Y, Enomoto. T, Yamaji. R, Miyatake. K, Inui. H, Nakano. Y.

    Biochim. Biophys. Acta 雑誌   vol.1426   126 - 132   1999.12

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  • Inhibition of aldose reductases by a- and b-glucosides from a Perilla and possible implications for recognition of a- and b-glucoside configuration Reviewed

    Fujita. T, Ohira. K, Takenoshita. M, Nakatsuka. T, Yamaji. R, Inui. H, Ueda. M, Miyatake. K, Nakayama. M, Nakano. Y.

    Appl. Biol. Sci. 雑誌   Vol. 4   123 - 134   1998.12

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  • Biological activity of hydroxovitamin B12 degradation product formed during microwave heating Reviewed

    Watanabe. F, Abe. K, Katsura. H, Takenaka. S, Mazumder. S.A.M.Z.H(学生), Yamaji. R, Ebara. S, Fujita. T, Tanimori. S, Kirihata. M, Nakano. Y.

    J. Agric. Food Chem. 雑誌   vol.46   5177 - 5180   1998.12

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  • Suppression by water extracts of Sophora plants of sucrose-induced hyperglycemia in rats and inhibition of intestinal disaccharidases in vitro Reviewed

    Shi. H.-C.Z(学生). Huang. Q.-Y, Yamaji. R, Inui. H, Fujita. T, Miyatake. K, Nakano. Y, Tada. T, Nishimura. K.

    Biosci. Biotechnol. Biochem. 雑誌   vol.62   1225 - 1227   1998.12

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  • The intron 5-inserted form of rat erythropoietin receptor is expressed as a membrane-bound form. Reviewed

    Yamaji. R, Murakami. C, Takenoshita. M, Tsuyama. S, Inui. H, Miyatake. K, Nakano. Y.

    Biochim. Biophys. Acta 雑誌   vol.1403   169 - 178   1998.12

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  • Low sucrase activity in the small intestine of a senescence-accelerated strain of mouse, SAMP1 Reviewed

    Takenoshita. M(学生), Yabune. M, Katsura. H, Yamaji. R, Inui. H, Miyatake. K, Nakano. Y.

    Biosci. Biotechnol. Biochem. 雑誌   vol.62   965 - 969   1998.12

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  • a2-Adrenoceptor-mediated antisecretory effect of hypoxia in conscious rats. Reviewed

    Yamaji. R, Ohnishi. Y(学生), Sakamoto. M, Takenoshita M, Ohta. M, Tsuyama. S, Inui. H, Miyatake. K, Nakano. Y.

    Biosci. Biotechnol. Biochem. 雑誌   vol.62   546 - 549   1998.12

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  • Suppressive effect of insulin on the synthesis of sucrase-isomaltase complex in small intestinal epithelial cells, and abnormal increase in the complex under diabetic conditions Reviewed

    Takenoshita. M(学生), Yamaji. R, Inui. H, Miyatake. K, Nakano. Y.

    Biochem. J. 雑誌   vol.329   597 - 600   1998.12

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  • Isolation of two isoflavone glycosides from sophora plants and their inhibitory effects on small intestinal disaccharidases Reviewed

    Shi. H.-C(学生), Huang. Q.-Y, Yamaji. R, katsura. H, Inui. H, Takenoshita. M, Tanimori. S, Fujita. T, Miyatake. K, Nakano. Y, Tada. T, Nishimura. K.

    Appl. Biol. Sci. 雑誌   Vol. 3   95 - 102   1997.12

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  • Inositol 1,4,5-triphosphate and cyclic ADP-ribose mobilize Ca2+ in a protist, Euglena gracilis Reviewed

    Masuda. W(学生), Takenaka. S, Tsuyama. S, Tokunaga. M, Yamaji. R, Inui. H, Miyatake. K, Nakano. Y.

    Comp.Biochem. Physiol. 雑誌   vol.18   279 - 283   1997.12

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  • Hypoxia inhibits gastric emptying and gastric acid secretion in conscious rats Reviewed

    Yamaji. R, Sakamoto. M(学生), Miyatake. K, Nakano. Y.

    J. Nutr. 雑誌   vol.126   673 - 680   1996.12

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  • Mitochondrial NADH- or NADPH-linked aquacobalamin reductase activity is low in human skin fibroblasts with inherited disorders in synthesis of cobalamin coenzymes Reviewed

    Watanabe. F, Saido. H, Yamaji. R, Miyatake. K, Isegawa. Y, Ito. A, Yubisui. T, Rosenblatt. D. S, Nakano. Y.

    J. Nutr. 雑誌   vol.126   2947 - 2951   1996.12

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  • Brain capillary endothelial cells express two forms of erythropoietin receptor mRNA Reviewed

    Yamaji. R, Okada. T(学生), Moriya. M, Naito. M, Tsuruo. T, Miyatake. K, Nakano. Y.

    Eur. J. Biochem. 雑誌   vol.239   494 - 500   1996.12

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  • A protist, Euglena gracilis Z, Functions as a sole nutrient source in a closed ecosyste Reviewed

    Nakano. Y, Miyatake. K, Yamaji. R, Nishizawa. A, Shigeoka. S, Hosotani. K, Inui. H, Watanabe. F, Enomoto. T, Takenaka. S.

    CELSS J. 雑誌   8   1995.12

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  • Characteristics and the utilization of bio-particles, paramylon, formed by protist Reviewed

    Miyatake. K, Takenaka. S, Yamaji. R, Nakano. Y.

    J. Soc. Powder Technol. Japan 雑誌   32   566 - 572   1995.12

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  • Effect of thiamin on the content of fructose 2,6-bisphosphate in Euglena Reviewed

    Yamaji. R, Miyatake. K, Enomoto. T, Inui. H, Shigeoka. S, Kitaoka. S, Nakano. Y.

    Int. J. Biochem. 雑誌   vol.26   85 - 91   1994.12

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  • Characterization of aquacobalamin reductase (NADPH) from Euglena gracilis Reviewed

    Watanabe. F, Yamaji. R, Isegawa. Y, Yamamoto. T, Tamura. Y, Nakano. Y.

    Arch. Biochem. Biophys. 雑誌   vol.305   421 - 427   1993.12

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  • Pyruvate-NADP+ oxidoreductase from Euglena gracilis: Limited proteolysis of the enzyme with trypsin Reviewed

    Inui, H., Yamaji, R., Saido, H., Miyatake, K., Nakano, Y., Kitaoka, S.

    Arch. Biochem. Biophys. 雑誌   286 ( 1 )   270 - 276   1991.04

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Books and Other Publications

  • ビタミン・バイオファクター総合事典「カロテノイド 」

    山地亮一、北風智也 他( Role: Joint author)

    朝倉書店  2021.07 

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    Responsible for pages:291-292と304-306  

  • Inhibitory effects of eucalyptus and banaba leaf extracts on nonalcoholic steatohepatitis induced by a high-fructose/high-glucose diet in rats.

    Takahashi Y, Sugimoto K, Soejima Y, Kumagai A, Koeda T, Shojo A, Nakagawa K, Harada N, Yamaji R, Inui H, Yamanouchi T, and Fukusato T.( Role: Joint author)

    Prime Archives in Biomedical Sciences (VIDE LEAF)  2020.08 

  • 代謝センシング~健康、食、美容、薬そして脳の代謝を知る~第11章 カロテンとその代謝物による骨格筋へのヘルスベネフィット

    北風智也、原田直樹、山地亮一( Role: Joint author)

    シーエムシー出版  2019.09 

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    Responsible for pages:97-104  

  • 非栄養素の分子栄養学:骨格筋量とエストロゲン受容体βアゴニスト―大豆イソフラボンの可能性

    山地亮一( Role: Joint author)

    建帛社  2017.05 

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    Responsible for pages:155-169  

  • 女性の疾患と美容のための機能性素材の開発

    小川 真弘,原田 直樹,山地 亮一( Role: Joint author)

    シーエムシー出版   2014.07 

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    Responsible for pages:41652  

  • Effect of oxygen depletion on the food digestion. in "Functional Foods for Disease Prevention II"

    山地亮一( Role: Joint author)

    ACS Symposium Series, American Chemical Society, Washington D. C.  1998.12 

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    Responsible for pages:251-255  

  • Effects of aging and vitamin A deficiency on DNA damage in "Food Factors for Cnacer Prevention"

    山地亮一( Role: Joint author)

    Springer-Verlag, Tokyo  1997.12 

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    Responsible for pages:63-66  

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MISC

  • Fatty acid amides in breast milk and cognitive development Reviewed International journal

    98 ( 3 )   121 - 128   2024.03

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    Authorship:Last author, Corresponding author   International / domestic magazine:Domestic journal  

  • 卵巣老化のメカニズムと食品成分による予防 International journal

    田中 千智, 北風 智也, 原田 直樹, 山地 亮一

    ビタミン   97 ( 2 )   79 - 83   2023.02( ISSN:0006-386X

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    Authorship:Last author   International / domestic magazine:Domestic journal  

  • 骨格筋における異所性脂肪蓄積に対するレチノイン酸受容体アゴニストの抑制効果 International journal

    辰巳 理奈, 北風 智也, 原田 直樹, 山地 亮一

    ビタミン   96 ( 5-6 )   239 - 243   2022.06( ISSN:0006-386X

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    Authorship:Last author, Corresponding author   International / domestic magazine:Domestic journal  

  • クルクミンの生理作用解析ツールとしての代謝産物および構造類似体

    荒堀 有美, 原田 直樹, 山地 亮一

    ビタミン   95 ( 5-6 )   286 - 289   2021.06( ISSN:0006-386X

  • Gタンパク質共役エストロゲン受容体の活性化による抗肥満効果 Reviewed

    佐々木里那,原田直樹,山地亮一

    ビタミン   95 ( 1 )   20 - 23   2021.01

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  • アピゲニンによる骨格筋量の調節機構

    前川 大輔, 原田 直樹, 山地 亮一

    ビタミン   94 ( 7 )   406 - 409   2020.07( ISSN:0006-386X

  • α-カロテン、β-カロテン、β-クリプトキサンチンなどのプロビタミンA:カロテノイドの健康機能に関して、最近の動向 Reviewed

    山地亮一

    日本食品安全協会会誌   15 ( 2 )   71 - 77   2020.02

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  • 抗肥満効果をもつirisinのビタミンAによる発現制御 Reviewed

    吉川実樹、原田直樹、山地亮一

    ビタミン   93 ( 10 )   444 - 447   2019.10

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  • 骨格筋の健康におけるメトキシフラボンの有用性

    山地 亮一

    ビタミン   93 ( 7 )   299 - 302   2019.07( ISSN:0006-386X

  • 筋分化におけるレチノイドX受容体の重要性

    山口真由, 原田直樹, 山地亮一

    ビタミン   93 ( 5/6 )   253‐256   2019.06( ISSN:0006-386X

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  • ヒト皮膚線維芽細胞の増殖と細胞外マトリックス発現におけるアポ型とホロ型ラクトフェリンの作用評価 Reviewed

    野上明日香、竜端之、原田直樹、鈴木靖志、山地亮一

    ラクトフェリン   47 - 52   2019.04

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  • 摂食量調節と代謝ホメオスタシス制御における脂肪酸アミドの生理機能

    小林恭之, 原田直樹, 山地亮一

    ビタミン   93 ( 2 )   66‐69   2019.02( ISSN:0006-386X

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  • ユーグレナの明暗条件下での生育における内分泌撹乱物質;p-ノニルフェノール(PNP)毒性低減化について

    大桑(林)浩孝, 大桑(林)浩孝, 原田直樹, 山地亮一, 岡井康二, 阪本龍司, 阪本龍司, 中野長久, 中野長久

    ビタミン   93 ( 4 )   2019( ISSN:0006-386X

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  • 抗肥満効果をもつirisinのビタミンAによる発現制御

    吉川実樹, 原田直樹, 山地亮一

    ビタミン   93 ( 1 )   444 - 447   2019

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Presentations

  • 骨格筋におけるトランスグルタミナーゼ2の運動による発現調節機構 Domestic conference

    道菅まなみ、岸優樹、杉本優季、北風智也、原田直樹、山地亮一

    日本栄養・食糧学会近畿支部大会  2023.11  日本栄養・食糧学会

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    Presentation type:Oral presentation (general)  

    Venue:奈良  

  • オレアミドは狭小ケージで飼育したマウスにおいて誘発される肥満を抑制する Domestic conference

    窪田舞、小林恭之、杉本圭一郎、北風智也、原田直樹、山地 亮一

    日本栄養・食糧学会近畿支部大会  2023.11 

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    Presentation type:Oral presentation (general)  

    Venue:奈良  

  • オレアミドの肥満抑制効果 Domestic conference

    山地亮一

    第473回ビタミンB研究協議会  2023.11  日本ビタミン学会ビタミンB研究協議会

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    Venue:京都  

  • 膵臓β細胞におけるRedd2発現制御とノックアウトマウスを用いた機能解析 Domestic conference

    山田志帆、浦川夏帆、原田直樹、北風智也、松村成暢、真下知士、乾博、山地亮一

    日本農芸化学会関西支部例会  2023.07 

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    Presentation type:Oral presentation (general)  

    Venue:大阪(大阪公立大学)  

  • 筋肥大促進効果を有するレチノイン酸受容体γ標的遺伝子トランスグルタミナーゼ2の運動による発現調節機構 Domestic conference

    北風智也、岸優樹、杉本優季、道菅まなみ、原田直樹、山地亮一

    日本ビタミン学会大会  2023.06 

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    Presentation type:Oral presentation (general)  

    Venue:宮城(東北大学)  

  • 運動による骨格筋量の増加におけるビタミンAシグナルの関与 Domestic conference

    中辻あいの、北風智也、原田直樹、山地亮一

    日本ビタミン学会大会  2023.06 

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    Presentation type:Oral presentation (general)  

    Venue:宮城(東北大学)  

  • 分泌タンパク質HtrA3の骨格筋における機能について Domestic conference

    北風智也、原田直樹、山地亮一

    日本栄養・食糧学会大会  2023.05 

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    Presentation type:Poster presentation  

    Venue:北海道(札幌コンベンションセンター)  

  • メスマウスにおいてS-エクオール摂取は長趾伸筋の筋力を増加させる Domestic conference

    佐藤衣織,小原亜希子,卯川裕一,松山彰収,北風智也,原田直樹,山地亮一

    日本栄養・食糧学会大会  2023.05 

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    Presentation type:Poster presentation  

    Venue:北海道(札幌コンベンションセンター)  

  • S-エクオールへの水酸化によるエストロゲン受容体活性化に対する影響の変化 Domestic conference

    大西照晃、原田直樹、北風智也、卯川裕一、松山彰収、古屋俊樹、山地亮一

    日本栄養・食糧学会大会  2023.05 

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    Presentation type:Poster presentation  

    Venue:北海道(札幌コンベンションセンター)  

  • 運動による骨格筋量の増加におけるビタミンAシグナルの関与 Domestic conference

    中辻 あいの, 北風 智也, 原田 直樹, 山地 亮一

    ビタミン  2023.04  (公社)日本ビタミン学会

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    Presentation type:Oral presentation (general)  

  • 筋肥大促進効果を有するレチノイン酸受容体γ標的遺伝子トランスグルタミナーゼ2の運動による発現調節機構 Domestic conference

    北風 智也, 岸 優樹, 杉本 優季, 道菅 まなみ, 原田 直樹, 山地 亮一

    ビタミン  2023.04  (公社)日本ビタミン学会

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    Presentation type:Oral presentation (general)  

  • 筋分化におけるLGR6の役割とその発現調節機構 Domestic conference

    辰巳理奈、山口真由、北風智也、原田直樹、山地亮一

    日本農芸化学会大会  2023.03 

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    Presentation type:Oral presentation (general)  

  • アンドロゲンによるシアル酸転移酵素発現調節を介した口腔内細菌叢の性差形成 Domestic conference

    原田 直樹、出南 真奈、北風 智也、乾 博、山地 亮一

    日本農芸化学会大会  2023.03 

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  • 甲状腺ホルモン受容体αの非古典的作用による筋分化促進作用 Domestic conference

    玉木公平、網干舞音、北風智也、原田直樹、山地亮一

    日本農芸化学会大会  2023.03 

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  • 食品成分の機能性による骨格筋の健康へのアプローチ Domestic conference

    山地亮一

    6大学共催フォーラム第12回  2023.03 

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    Presentation type:Symposium, workshop panel (nominated)  

  • 分泌タンパク質HtrA3の骨格筋における機能について Domestic conference

    北風 智也, 原田 直樹, 山地 亮一

    日本栄養・食糧学会大会講演要旨集  2023.03  (公社)日本栄養・食糧学会

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  • メスマウスにおいてS-エクオール摂取は長趾伸筋の筋力を増加させる

    佐藤 衣織, 小原 亜希子, 卯川 裕一, 松山 彰収, 北風 智也, 原田 直樹, 山地 亮一

    日本栄養・食糧学会大会講演要旨集  2023.03  (公社)日本栄養・食糧学会

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  • S-エクオールへの水酸化によるエストロゲン受容体活性化に対する影響の変化 Domestic conference

    大西 照晃, 原田 直樹, 北風 智也, 小原 亜希子, 卯川 裕一, 松山 彰収, 古屋 俊樹, 山地 亮一

    日本栄養・食糧学会大会講演要旨集  2023.03  (公社)日本栄養・食糧学会

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  • Mogrol derived from Siraitia grosvenorii activates the bile acid receptor TGR5 and suppresses hyperglycemia in high-fat diet-fed KKAy mice International conference

    Chisato Tanaka, Hiroki Urushizaki, Yoshiaki Teraoka, Tomoya Kitakaze, Yuta Ito, Yuji Murata, Takashi Inui, Hiroshi Inui, Naoki Harada, Ryoichi Yamaji

    2nd IUNS-ICN International Congress of Nutrition   2022.12 

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  • Vitamin A-responsive LGR6 promotes myoblast differentiation International conference

    Rina Tatsumi, Mayu Yamguchi, Tomoya Kitakaze, Naoki Harada, Ryoichi Yamaji

    22nd IUNS-ICN International Congress of Nutrition   2022.12 

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  • Carotenoid transporter CD36 expression depends on hypoxia-inducible factor-1 α under normal physiological conditions in the soleus muscles of mice International conference

    Tomoya Kitakaze, Takashi Sugihira, Hiromichi Kameyama, Asami Maruchi, Yasuyuki Kobayashi, Naoki Harada, Ryoichi Yamaji

    22nd IUNS-ICN International Congress of Nutrition  2022.12 

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  • Identification of G protein-coupled receptors GPR55 and GPR97 as molecular targets of curcumin and involvement of GPR55 in the antidiabetic function of curcumin International conference

    aoki Harada, Mai Okuyama, Yoshiaki Teraoka, Yumi Arahori, Tomoya Kitakaze, Takashi Inui, Naoki Goshima, Claus Schneider, Hiroshi Inui, Ryoichi Yamaji

    2nd IUNS-ICN International Congress of Nutrition   2022.12 

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  • 雄マウス褐色脂肪組織での熱産生におけるアンドロゲン受容体の作用とその分子機構 Domestic conference

    大西 照晃, 久保 慶太朗, 原田 直樹, 北風 智也, 後藤 剛, 乾 博, 山地 亮一

    日本生化学会大会  2022.11  (公社)日本生化学会

  • フルクトースの飲水摂取は膵β細胞減少を伴う耐糖能異常を誘発する Domestic conference

    新森 耀、原田直樹、杉本圭一郎、北風智也、乾博、山地亮一

    日本栄養・食糧学会近畿支部大会  2022.10 

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    Venue:滋賀(滋賀県立大学)  

  • レチノイン酸応答遺伝子トランスグルタミナーゼ2の運動による発現調節機構について Domestic conference

    北風智也, 岸優樹, 中辻あいの, 原田直樹, 山地亮一

    日本栄養・食糧学会近畿支部大会  2022.10 

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    Venue:滋賀(滋賀県立大学)  

  • β-caroteneによるヒラメ筋重量の増加機構 Domestic conference

    岡野 結衣, 杉平 貴史, 亀山 大道, 丸地 麻美, 北風 智也, 原田 直樹, 山地 亮一

    日本ビタミン学会大会  2022.06  (公社)日本ビタミン学会

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  • メトキシフラボンによるGPR97の活性化について Domestic conference

    山地 亮一

    第467回ビタミンB研究協議会  2022.06  (公社)日本ビタミン学会

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    Venue:福岡(JR博多シティ)  

  • メスマウスのヒラメ筋量に及ぼすS-エクオールの効果 Domestic conference

    佐藤 衣織, 佐々木 里那, 卯川 裕一, 松山 彰収, 北風 智也, 原田 直樹, 山地 亮一

    日本栄養・食糧学会大会  2022.06  (公社)日本栄養・食糧学会

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  • 唾液ムチン10のシアル酸修飾に対するアンドロゲンの作用 Domestic conference

    原田 直樹, 出南 真奈, 北風 智也, 乾 博, 山地 亮一

    日本栄養・食糧学会大会  2022.06  (公社)日本栄養・食糧学会

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  • メトキシフラボンによる骨格筋肥大促進におけるGPR97-SRFシグナルの関与 Domestic conference

    木村 彩乃, 前川 大輔, 北風 智也, 原田 直樹, 山地 亮一

    日本栄養・食糧学会大会  2022.06  (公社)日本栄養・食糧学会

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  • オレアミドの腸管吸収に対するフラボノイド類の影響 Domestic conference

    日浦 伶菜, 小林 恭之, 渡辺 夏美, 室田 佳恵子, 中村 衣里, 松浦 寿喜, 杉本 圭一郎, 北風 智也, 原田 直樹, 山地 亮一

    日本栄養・食糧学会大会  2022.06  (公社)日本栄養・食糧学会

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  • 膵臓β細胞の小胞体ストレス誘導性アポトーシスに対するインスリンの抑制作用とその機構の解明 Domestic conference

    村田 菜々子, 西村 佳那, 原田 直樹, 北風 智也, 乾 博, 山地 亮一

    日本栄養・食糧学会大会  2022.06  (公社)日本栄養・食糧学会

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  • 膵β細胞における酸化ストレスによるRedd2の発現調節と個体レベルでの役割 Domestic conference

    坂本 修哉, 浦川 夏帆, 田宮 央登, 原田 直樹, 山地 亮一, 北風 智也, 乾 博, 松村 成暢, 真下 知士

    日本栄養・食糧学会大会  2022.06  (公社)日本栄養・食糧学会

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  • 骨格筋の健康の維持・増進におけるカロテノイドの作用機構 Domestic conference

    山地亮一

    第24回健康栄養シンポジウム 日本栄養・食糧学会関東支部  2022.03 

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  • 膵臓β細胞における酸化ストレスによるRedd2の発現調節機構とその役割の解明 Domestic conference

    浦川夏帆、田宮央登、原田直樹、松村成暢、真下知士、北風智也、乾博、山地亮一

    日本農芸化学会2022年度大会  2022.03 

  • GPR55活性化物質としてのクルクミンの同定とインクレチン分泌における役割 Domestic conference

    原田直樹、奥山真衣、寺岡佳晃、荒堀有美、新森耀、北風智也、乾隆、 乾博、山地亮一

    日本農芸化学会2022年度大会  2022.03 

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  • オレアミドは狭小ケージで行動範囲を制限したマウスにおいて誘発される肥満を抑制する Domestic conference

    窪田 舞,小林 恭之,杉本 圭一郎,北風 智也,原田 直樹,山地 亮一

    日本農芸化学会2022年度大会  2022.03 

  • 筋分化における古典的・非古典的な甲状腺ホルモン受容体α作用の役割 Domestic conference

    網干 舞音,古川 潤,北風 智也,原田 直樹,山地 亮一

    日本農芸化学会2022年度大会  2022.03 

  • 褐色脂肪組織での熱産生に及ぼす男性ホルモンの作用とそのメカニズム Domestic conference

    久保慶太朗、原田直樹、北風智也、乾博、山地亮一

    日本農芸化学会2022年度大会  2022.03 

  • 非アルコール性脂肪性肝炎モデルマウスに対するカフェインやクロロゲン酸の作用 Domestic conference

    宇津木 良丞, ドゥングバト・エルデネツォグト , 渡部 朱織, 富樫 有紗, 渡邉 雅人, 小林 恭之, 原田 直樹, 山地 亮一, 福里 利夫, 高橋 芳久

    日本病理学会会誌  2022.03  (一社)日本病理学会

  • 座りがちな生活習慣において骨格筋と内臓脂肪を標的とする一次脂肪酸アミドの活用 Domestic conference

    山地亮一

    メディカルジャパン大阪2022   2022.02 

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    Venue:大阪(インテックス大阪)  

  • 5. メトキシフラボンによる GPR97 の活性化について Domestic conference

    山地 亮一

    ビタミン  2022  公益社団法人 日本ビタミン学会

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    DOI: 10.20632/vso.96.11_488_1

  • 骨格筋の筋分化におけるLGR6の発現調節機構の解析 Domestic conference

    巳理奈,山口真由,北風智也,原田直樹,乾博,山地亮一

    日本ビタミン学会パート2  2021.11 

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  • 一級脂肪酸アミドであるオレアミドの 腸管における吸収経路と吸収機構について Domestic conference

    小林恭之, 渡辺夏美, 日浦伶奈, 室田佳恵子, 中村衣里, 松浦寿喜, 杉本圭一郎, 北風智也, 原田直樹, 山地亮一

    日本栄養・食糧学会近畿支部大会  2021.11 

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  • 異性化糖摂取マウスの膵β細胞機能に及ぼす食餌性状の影響

    原田 直樹, 野村 雅之, 與田 安紘, 北風 智也, 松村 成暢, 乾 博, 山地 亮一

    日本栄養・食糧学会大会  2021.07 

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  • 羅漢果由来mogrolのGPCR型胆汁酸受容体選択的アゴニストとしての同定とアルコール性臓器障害モデルマウスへの作用 Domestic conference

    田中千智、漆崎広喜、寺岡佳晃、井沢武史、原田直樹、北風智也、伊藤雄太、村田雄司、石黒正路、乾隆、乾博、山地亮一

    日本栄養・食糧学会大会  2021.07 

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  • 体温調節における男性ホルモンの作⽤とその分⼦機構の解明 Domestic conference

    久保慶太朗、原⽥直樹、北⾵智也、乾博、⼭地亮⼀

    日本農芸化学会関西支部例会  2021.07 

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  • 羅漢果由来mogrolのGPCR型胆汁酸受容体選択的アゴニストとしての同定とアルコール性臓器障害モデルマウスへの作用

    田中 千智, 漆崎 広喜, 寺岡 佳晃, 井澤 武史, 原田 直樹, 北風 智也, 伊藤 雄太, 村田 雄司, 石黒 正路, 乾 隆, 乾 博, 山地 亮一

    日本栄養・食糧学会大会講演要旨集  2021.07  (公社)日本栄養・食糧学会

  • 骨格筋の筋分化におけるLGR6の発現調節機構の解析 Domestic conference

    辰巳理奈,山口真由,北風智也,原田直樹,乾博,山地亮一

    日本ビタミン学会  2021.06 

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  • 唾液ムチン10 の糖鎖修飾と糖転移酵素発現に及ぼすアン Domestic conference

    出南真奈,原田直樹,乾博,山地 亮一

    日本農芸化学会2021年度大会  2021.03 

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  • 腸管におけるオレアミドの吸収機構 Domestic conference

    小林恭之,渡辺夏美,杉本圭一郎,室田 佳恵子,甲斐 建次,原田 直樹,乾 博,山地 亮一

    日本農芸化学会2021年度大会  2021.03 

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  • 腸内細菌叢を介したアンドロゲンのエネルギー代謝調節 Domestic conference

    原田直樹,花田一貴,南友香梨,乾博,山地 亮一

    日本農芸化学会2021年度大会  2021.03 

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  • メスマウスにおけるダイゼインによるヒラメ筋の肥大促 Domestic conference

    佐々木 里那,河田 夏初,原田 直樹,山地 亮一

    日本農芸化学会2021年度大会  2021.03 

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  • 5- ヒドロキシ-7- メトキシフラボンによるGPR97 活性 Domestic conference

    前川 大輔,原田 直樹,山地 亮一

    日本農芸化学会2021年度大会  2021.03 

  • メトキシフラボンによる筋管細胞肥 Domestic conference

    山地亮一

     第462回ビタミンB研究協議会  2021.03 

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  • 腸管における一級脂肪酸アミドの吸収機構

    山地 亮一

    第466回ビタミンB研究委員会  2021.03  (公社)日本ビタミン学会

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  • マウスの非アルコール性脂肪性肝炎に対する低用量エタノールの作用

    高橋 芳久, 渡部 朱織, 富樫 有紗, 渡邊 雅人, ドウングバット・エルデンソト , 小林 恭之, 原田 直樹, 山地 亮一, 杉本 圭一郎, 福里 利夫

    日本病理学会会誌  2021.03  (一社)日本病理学会

  • 骨格筋の制御機構:内在性、液性因子による可塑性の新展開 ビタミンA前駆体による骨格筋の健康

    山地 亮一

    体力科学  2021.02  (一社)日本体力医学会

  • オレアミドの腸管吸収機構の解析 Domestic conference

    渡辺夏美、小林恭之、杉本圭一郎、室田佳恵子、原田直樹、乾博、山地亮一

    日本栄養・食糧学会近畿支部大会  2020.11 

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  • 膵β細胞における小胞体ストレス誘導性アポトーシスに対するインスリン Domestic conference

    西村佳那、原田直樹、乾博、山地亮一

    日本栄養・食糧学会近畿支部大会  2020.11 

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  • クルクミンによるGPR97活性化機構の解明 Domestic conference

    荒堀有美、原田直樹、五島直樹、乾博、山地亮一

    日本栄養・食糧学会近畿支部大会  2020.11 

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  • ヒラメ筋におけるカロテノイドトランスポーターCD36の発現調節機構の解析 Domestic conference

    丸地麻美、杉平貴史、亀山大道、北風智也、原田直樹、乾博、山地亮一

    日本栄養・食糧学会近畿支部大会  2020.11 

  • ビタミンA前駆体による骨格筋の健康 Domestic conference

    山地亮一

    日本体力医学会大会  2020.09 

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  • 骨格筋におけるレチノイン酸応答遺伝子トランスグルタミナーゼ2の発現調節機構の解析 Domestic conference

    岸優樹、吉川実樹、北風智也、原田直樹、山地亮一

    日本ビタミン学会大会  2020.06 

  • カロテノイドを利用した骨格筋の健康維持・増進 Invited Domestic conference

    山地亮一

    日本栄養・食糧学会大会 医学系学会(日本リハビリテーション栄養学会)との合同シンポジウム  2020.05 

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    Presentation type:Oral presentation (invited, special)  

  • アンドロゲン受容体ノックアウト雄性マウスの⾼脂肪⾷摂取に起因したエネルギー代謝異常への腸内細菌叢の関与 Domestic conference

    原⽥ 直樹、花⽥ ⼀貴、南 友⾹梨、乾 博、⼭地 亮⼀

    日本栄養・食糧学会大会  2020.05 

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  • Oleamideは行動範囲を制限したマウスの前脛骨筋萎縮を改善する Domestic conference

    小林恭之、渡辺夏美、甲斐建次、杉本圭一郎、原田直樹、山地亮一

    日本栄養・食糧学会大会  2020.05 

  • 膵β細胞におけるRedd2の酸化ストレスによる発現調節とその役割 Domestic conference

    小林恭之、渡辺夏美、甲斐建次、杉本圭一郎、原田直樹、山地亮一

    日本栄養・食糧学会大会  2020.05 

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  • アロニア果汁の脂肪蓄積抑制メカニズム

    山根 拓也, 今井 ももこ, 小塚 美由記, 飯田 聡史, 原田 直樹, 竹中 重雄, 山地 亮一, 阪本 龍司, 石田 哲夫, 乾 博, 中垣 剛典, 中野 長久

    日本栄養・食糧学会大会講演要旨集  2020.04  (公社)日本栄養・食糧学会

  • メトキシフラボンによる筋管細胞肥大におけるGPCRの関与

    山地 亮一

    ビタミン  2020.03  (公社)日本ビタミン学会

  • 骨格筋におけるメトキシフラボンによるタンパク質合成促進について

    前川 大輔, 山地 亮一

    ビタミン  2019.09  (公社)日本ビタミン学会

  • アロニア果汁含有脂肪蓄積抑制物質の効果

    山根 拓也, 今井 ももこ, 小塚 美由記, 飯田 聡史, 原田 直樹, 石田 哲夫, 竹中 重雄, 山地 亮一, 阪本 龍司, 乾 博, 山本 好男, 大久保 岩男, 中垣 剛典, 中野 長久

    日本生化学会大会プログラム・講演要旨集  2019.09  (公社)日本生化学会

  • 骨格筋におけるoleamideによるタンパク質分解機構の解析

    渡辺 夏美, 小林 恭之, 北風 智也, 杉本 圭一郎, 原田 直樹, 乾 博, 山地 亮一

    ビタミン  2019.04  (公社)日本ビタミン学会

  • 遅筋におけるカロテノイドトランスポーターCD36の発現調節機構の解析

    丸地 麻美, 杉平 貴史, 亀山 大道, 北風 智也, 原田 直樹, 乾 博, 山地 亮一

    ビタミン  2019.04  (公社)日本ビタミン学会

  • 胎児期雄性ラットにおける男性ホルモンの作用抑制が耐糖能に及ぼす影響について

    原田 直樹, 四元 優佑, 乾 博, 山地 亮一

    日本栄養・食糧学会大会講演要旨集  2019.04  (公社)日本栄養・食糧学会

  • Oleamideは行動範囲制限マウスの骨格筋におけるmTOR経路を活性化する

    小林 恭之, 北風 智也, 杉本 圭一郎, 原田 直樹, 乾 博, 山地 亮一

    日本栄養・食糧学会大会講演要旨集  2019.04  (公社)日本栄養・食糧学会

  • 5-ヒドロキシ-7-メトキシフラボンはヒラメ筋のタンパク質合成を促進する

    前川 大輔, 吉田 直樹, 原田 直樹, 大桑 浩孝[林], 藤田 貴則, 中野 長久, 山地 亮一

    日本栄養・食糧学会大会講演要旨集  2019.04  (公社)日本栄養・食糧学会

  • 脂肪酸アミドによる骨格筋量調節について

    小林 恭之, 山地 亮一

    ビタミン  2019.02  (公社)日本ビタミン学会

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Works

  • 骨格筋の分化・肥大あるいは萎縮を調節する因子の同定とその作用機構

    山地 亮一

    2008
    -
    Now

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    加齢あるいは寝たきりにより筋力の低下は起こる。骨格筋を形成する筋細胞の増殖能と分化能の低下あるいは筋萎縮がその原因となるが、その詳細は不明である。そこで筋細胞の増殖・分化を促進または抑制する因子、肥大または萎縮を促進する因子を同定し、それらの作用機構を解明する。現在筋分化促進因子または抑制因子、肥大因子または萎縮促進因子として同定したタンパク質の機能を解析しつつ、そのタンパク質の機能をin vitroで評価するシステムを構築している。評価系が構築できれば筋分化抑制因子あるいは萎縮促進因子の機能を阻害する物質のスクリーニングに利用可能となる。

  • アンドロゲン様活性物質/抗アンドロゲン様活性物質の探索と機能性食品(または化粧品、医薬品)への応用

    山地 亮一

    2000
    -
    Now

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    加齢に伴い男性ホルモンは低下し、その結果として男性の更年期障害、筋力の低下、男性骨粗鬆症が起こるので、非栄養 素成分として機能するアンドロゲン様活性(アンドロゲン受容体に対する活性)物質を食経験のある植物から探索・同定する。一方、高齢男性の前立腺肥大と前立腺癌の罹患率は上昇しているので、抗アンドロゲン様活性(アンドロゲン受容体に対する阻害活性)物質を食経験のある植物から探索・同定する(現在、抗アンドロゲン活性物質としてブドウの果皮に含まれるレスベラトロールに注目している)。さらにこれらの生理活性物質の詳細な作用機構を解明し、機能性食品(または化粧品、医薬品)への応用を目指している。

  • エストロゲン様生理活性物質/抗エストロゲン様生理活性物質のスクリーニング系の構築

    山地 亮一

    2000
    -
    Now

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    エストロゲン様生理活性物質/抗エストロゲン様生理活性物質をスクリーニングするバイオアッセイ系を酵母と動物細胞で構築し、ハイスループットな評価系を目指している。

  • エストロゲン様活性物質/抗エストロゲン様活性物質の探索と機能性食品(または化粧品、医薬品)への応用

    2000
    -
    Now

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    大豆イソフラボン(ダイゼインやゲニステイン)はエストロゲン受容体βに作用して女性の更年期障害、骨粗鬆症などの疾病を予防するので、食経験のある植物からエストロゲン様活性(特にエストロゲン受容体βに対する活性)物質を探索し、同定している。一方、エストロゲン依存性の癌(乳癌など)ではエストロゲン受容体αへのエストロゲンの結合によって細胞増殖が促進するので、エストロゲン受容体α対する阻害物質を植物から探索し、同定している。さらにこれらの生理活性物質の詳細な作用機構を解明し、機能性食品(または化粧品、医薬品)への応用を目指している。

  • アンドロゲン様生理活性物質/抗アンドロゲン様生理活性物質のスクリーニング系の構築

    山地 亮一

    2000
    -
    Now

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    アンドロゲン様生理活性物質/抗アンドロゲン様生理活性物質をスクリーニングするバイオアッセイ系を酵母または動物細胞で構築し、ハイスループットな評価系を目指している。

  • 低酸素応答に関連する因子の機能解析

    山地 亮一

    1993
    -
    Now

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    低酸素環境下で生育する癌細胞は、その環境に適応して増殖するために低酸素応答遺伝子の発現を促進する。本研究は、低酸素応答の主要な因子であるHIFの発現を抑制する因子(癌細胞増殖抑制因子、血管新生阻害因子)を探索し、その因子による癌細胞の増殖抑制を検討している。

  • 骨格筋の増殖・分化・肥大を促進、または萎縮を抑制する食品成分の同定とその作用機構

    山地 亮一

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    加齢あるいは寝たきりにより筋力の低下は起こる。運動以外に日々摂取する食事は骨格筋を量的・質的に維持・増強するために役立つ。そこで骨格筋を形成する筋細胞の増殖・分化・肥大を促進する、あるいは萎縮を抑制する食品成分を同定し、それらの作用機構を解明する。

  • 骨格筋萎縮因子としての脱ユビキチンの役割

    山地 亮一

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    骨格筋萎縮因子として同定した脱ユビキチン化酵素(USP19)の機能の解明とin vitroでの活性測定系の確立を目指している。この測定系の確立は阻害剤探索に利用できる。

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Industrial Property Rights

  • 糖及び/又は脂質代謝改善剤

    山地亮一、小林恭之、窪田舞、杉本圭一郎

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    property_type:Patent 

    Application no:2021-187256 

  • オートファジー活性向上剤

    向井克之、山地亮一、原田直樹、野口真里

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    property_type:Patent 

    Application no:特願2018-118259 

  • 骨格筋強化剤

    山地亮一、甲斐建次、小林恭之、杉本圭一郎

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    property_type:Patent 

    Application no:P 2018100069  

    Announcement no:P2019202969  

    Patent/Registration no:特許第7193073号 

  • ラクトフェリンを含有する筋肉の損傷治癒促進組成物と適用方法

    龍見 宗樹、鈴木 靖志、山地 亮一、原田 直樹

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    property_type:Patent 

    Application no:特願2016-035317 

  • 脂質蓄積抑制効果を有する新規羅漢果抽出物組成物

    伊藤 雄太、田端 宏充、鈴木 靖志、乾 博、山地 亮一、原田 直樹

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    property_type:Patent 

    Application no:特願2015-50430 

    Patent/Registration no:5864003 

  • 筋量増加剤

    山地亮一、林浩孝、藤田貴則

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    property_type:Patent 

    Patent/Registration no:5917450 

  • アピゲニンの産生方法

    稲福桂一郎、比嘉光、山地亮一

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    property_type:Patent 

  • レスベラトロール・アフィニティ担体

    山地亮一、谷森紳治、原田直樹

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    property_type:Patent 

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Collaborative research (seeds) keywords

  • 骨格筋の肥大促進と萎縮抑制に関与する食品成分や生体成分の評価

  • 食品成分の機能性の探索と解析(機能性食品成分)

  • 骨格筋の分化促進と筋萎縮抑制物質の探索

  • 低酸素誘導因子(HIF)を介した低酸素応答遺伝子発現活性化の測定法

  • アンドロゲン及び抗アンドロゲン様生理活性の測定法

  • エストロゲン及び抗エストロゲン様生理活性の測定法

  • 動物細胞を用いた機能性物質の探索

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Outline of collaborative research (seeds)

  • ロコモティブシンドロームとメタボリックシンドロームに対する対策としての骨格筋の量的・質的な維持と増強をサポートする機能性食品成分について

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    Request for collaborative research:The private sector, such as other institutions

    Type of research exchange:Technical consultation, Contract research, Joint research, Lecture

    加齢や寝たきりにより骨格筋は量的にも質的にも低下するので、骨格筋の肥大を促進し、あるいは萎縮を抑制することはロコモティブシンドロームとメタボリックシンドロームに対する対策となる。したがって骨格筋の肥大を促進、あるいは筋萎縮を予防する因子(機能性食品成分などを含める)を探索・同定し、in vitroとin vivo実験によってその詳細な機能を評価するとともに、機能性食品または創薬への応用を検討する。

  • 抗アンドロゲン様活性を持つ生理活性物質の探索と機能性食品または創薬への応用

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    アンドロゲン受容体に作用する抗アンドロゲン活性物質の探索とその機能の解明。抗アンドロゲン活性物質は男性ホルモンの関連する疾患(前立腺肥大や前立腺がん、男性型脱毛症)の予防に寄与するので、機能性食品成分から創薬まで幅広く活用される。

  • エストロゲン様または抗エストロゲン様活性を持つ生理活性物質の探索と機能性食品または創薬への応用

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    植物からエストロゲンあるいは抗エストロゲン様活性を持つ物質を探索し,その詳細な機能を解明し,機能性食品または創薬への応用を検討する。

  • 骨格筋の健康に関する生理活性物質の探索

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    生理活性物質(二糖類水解酵素阻害剤、癌細胞増殖抑制因子、血管新生阻害因子、抗酸化物質)の探索。

  • 栄養素およびバイオファクターのと機能解析

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    食品の栄養価の評価、癌細胞増殖抑制因子、血管新生阻害因子の探索を動物細胞を用いて評価する

Grant-in-Aid for Scientific Research

  • 骨格筋量調節におけるビタミンAの新規機能の同定とその作用機構の解明

    Grant-in-Aid for Scientific Research(B)  2023

  • 脂質メディエーターとしての一級脂肪酸アミドの機能の確立

    Grant-in-Aid for Challenging Research (Pioneering)/(Exploratory)  2022

  • フラボノイドによるadhesion型GPCRを介した骨格筋量調節機構の解明

    Grant-in-Aid for Scientific Research(B)  2022

  • 脂質メディエーターとしての一級脂肪酸アミドの機能の確立

    2021

  • フラボノイドによるadhesion型GPCRを介した骨格筋量調節機構の解明

    2021

  • フラボノイドによるadhesion型GPCRを介した骨格筋量調節機構の解明

    Grant-in-Aid for Scientific Research(B)  2020

  • 脂質メディエーターとしての一級脂肪酸アミドの機能の確立

    Grant-in-Aid for Challenging Research (Pioneering)/(Exploratory)  2020

  • 転写因子RARγの制御下にある筋肥大誘導因子の同定とその分子機序の解明

    Grant-in-Aid for Scientific Research(B)  2019

  • 転写因子RARγの制御下にある筋肥大誘導因子の同定とその分子機序の解明

    Grant-in-Aid for Scientific Research(B)  2018

  • カロテノイド代謝酵素の未知機能から読み解く脂肪蓄積機構の解析

    Grant-in-Aid for Challenging Research (Pioneering)/(Exploratory)  2018

  • 転写因子RARγの制御下にある筋肥大誘導因子の同定とその分子機序の解明

    2017

  • カロテノイド代謝酵素の未知機能から読み解く脂肪蓄積機構の解析

    2017

  • 骨格筋量の性差制御因子の同定と食品成分による発現調節

    2016

  • 骨格筋量の性差制御因子の同定と食品成分による発現調節

    2015

  • 骨格筋量調節におけるβ-カロテンの役割と新規分子機構の解明

    2015

  • 天然物由来フラボン誘導体による骨格筋の維持・増強の基盤研究

    2014

  • 骨格筋量調節におけるβ-カロテンの役割と新規分子機構の解明

    2014

  • 骨格筋量の性差制御因子の同定と食品成分による発現調節

    2014

  • 筋形成におけるエストロゲンの役割と植物エストロゲンの効果

    2013

  • 天然物由来フラボン誘導体による骨格筋の維持・増強の基盤研究

    2013

  • 天然物由来フラボン誘導体による骨格筋の維持・増強の基盤研究

    2013

  • 筋形成におけるエストロゲンの役割と植物エストロゲンの効果

    2013

  • 筋形成におけるエストロゲンの役割と植物エストロゲンの効果

    2012

  • 低酸素誘導因子の発現を亢進する調節因子の機能解析と機能阻害評価系の構築

    2012

  • 筋形成におけるエストロゲンの役割と植物エストロゲンの効果

    2012

  • 低酸素誘導因子の発現を亢進する調節因子の機能解析と機能阻害評価系の構築

    2012

  • 筋形成におけるエストロゲンの役割と植物エストロゲンの効果

    2011

  • 低酸素誘導因子の発現を亢進する調節因子の機能解析と機能阻害評価系の構築

    2011

  • 筋形成におけるエストロゲンの役割と植物エストロゲンの効果

    2011

  • 低酸素誘導因子の発現を亢進する調節因子の機能解析と機能阻害評価系の構築

    2011

  • 食品成分の分子標的としてのアンドロゲン受容体シグナル伝達機構に関する研究

    2010

  • 低酸素誘導因子の発現を亢進する調節因子の機能解析と機能阻害評価系の構築

    2010

  • 食品成分の分子標的としてのアンドロゲン受容体シグナル伝達機構に関する研究

    2009

  • 食品成分の分子標的としてのアンドロゲン受容体シグナル伝達機構に関する研究

    2008

  • 癌特異的ERaコアクチベーターを利用した乳癌治療薬探索系の開発

    2008

  • 低酸素関連タンパク質のクロストークによるアンドロゲン応答機構の解明

    2007

  • 低酸素関連タンパク質のクロストークによるアンドロゲン応答機構の解明

    2006

  • 柿カロテノイドの吸収に及ぼす脂肪酸量や種類の影響とその吸収促進要因の解明

    2005

  • 多局在性GAPDHの細胞内輸送機構と新規機能の解明

    2004

  • 高等動物、特に肝臓におけるビタミンB12の新規な機能、細胞内情報伝達への関与

    2004

  • 多局在性GAPDHの細胞内輸送機構と新規機能の解明

    2003

  • 多局在性GAPDHの細胞内輸送機構と新規機能の解明

    2002

  • 脳毛細血管内皮細胞が発現する2つの型のエリスロポエチンレセプターの機能解明

    1997

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Charge of on-campus class subject

  • 応用生命科学特別研究実験CII

    2023     Graduate school

  • 応用生命科学特別研究実験CI

    2023     Graduate school

  • 生命機能化学研究実験2B

    2023     Graduate school

  • 生命機能化学特別研究実験1A

    2023     Graduate school

  • 生命機能化学特別研究実験1B

    2023     Graduate school

  • 生命機能化学特別研究実験2A

    2023     Graduate school

  • 生命機能化学特別研究実験2B

    2023     Graduate school

  • 生命機能化学研究実験1A

    2023     Graduate school

  • 農学概論

    2023   Weekly class   Undergraduate

  • 生命機能化学研究実験1B

    2023     Graduate school

  • 生命機能化学研究実験2A

    2023     Graduate school

  • 生命機能化学特論A

    2023   Weekly class   Graduate school

  • 生命機能化学概論

    2023   Weekly class   Undergraduate

  • 栄養生化学

    2023   Weekly class   Undergraduate

  • 生命機能科学ゼミナール1A

    2023     Graduate school

  • 生命機能科学ゼミナール1AB

    2023     Graduate school

  • 生命機能科学ゼミナール2A

    2023     Graduate school

  • 生命機能科学ゼミナール2B

    2023     Graduate school

  • 食品代謝栄養学特論

    2023   Weekly class   Graduate school

  • 生命機能化学研究プレゼンテーション

    2023   Intensive lecture   Graduate school

  • 科学英語B

    2023     Undergraduate

  • 卒業研究

    2023     Undergraduate

  • 細胞生物学

    2023   Weekly class   Undergraduate

  • 生化学1

    2023   Weekly class   Undergraduate

  • 科学英語A

    2023    

  • 応用生命科学特別研究実験CII

    2022     Graduate school

  • 農学概論

    2022   Weekly class   Undergraduate

  • 応用生命科学特別研究実験BI

    2022     Graduate school

  • 応用生命科学特別研究実験BII

    2022     Graduate school

  • 応用生命科学特別研究実験CI

    2022     Graduate school

  • 応用生命科学ゼミナールC

    2022    

  • 応用生命科学ゼミナールD

    2022     Graduate school

  • 生命機能化学特論A

    2022   Weekly class   Graduate school

  • 応用生命科学研究実験C

    2022     Graduate school

  • 応用生命科学研究実験D

    2022     Graduate school

  • 生命機能化学研究実験1A

    2022     Graduate school

  • 生命機能化学研究実験1B

    2022     Graduate school

  • 生命機能化学特別研究実験1A

    2022     Graduate school

  • 生命機能化学特別研究実験1B

    2022     Graduate school

  • 生命機能化学概論

    2022   Weekly class   Undergraduate

  • 栄養生化学

    2022   Weekly class   Undergraduate

  • 生命機能化学ゼミナール1B

    2022   Weekly class   Graduate school

  • 生命機能化学研究プレゼンテーション

    2022   Intensive lecture   Graduate school

  • 食品代謝栄養学特論

    2022   Weekly class   Graduate school

  • 分子細胞生物学

    2022   Weekly class   Undergraduate

  • 生化学1

    2022   Weekly class   Undergraduate

  • 科学英語A

    2022     Undergraduate

  • 科学英語B

    2022   Weekly class   Undergraduate

  • 卒業研究

    2022   Practical Training   Undergraduate

  • 生命機能科学ゼミナール1A

    2022   Weekly class   Graduate school

  • 応用生命科学特別研究実験CII

    2021   Practical Training  

  • 応用生命科学特別研究実験CI

    2021   Practical Training  

  • 応用生命科学特別研究実験BII

    2021   Practical Training  

  • 応用生命科学特別研究実験BI

    2021   Practical Training  

  • 応用生命科学特別研究実験AII

    2021   Practical Training  

  • 応用生命科学特別研究実験AI

    2021   Practical Training  

  • 応用生命科学研究実験D

    2021   Practical Training  

  • 応用生命科学研究実験C

    2021   Practical Training  

  • 応用生命科学研究実験B

    2021   Practical Training  

  • 応用生命科学研究実験A

    2021   Practical Training  

  • 応用生命科学プレゼンテーション

    2021    

  • 応用生命科学ゼミナールD

    2021    

  • 応用生命科学ゼミナールC

    2021    

  • 応用生命科学ゼミナールB

    2021    

  • 応用生命科学ゼミナールA

    2021    

  • 科学英語B

    2021    

  • 科学英語A

    2021    

  • 卒業研究

    2021   Practical Training  

  • Basic Biochemistry

    2021    

  • Molecular Cell Biology

    2021    

  • Introduction to Life, Environment, and Advanced Sciences

    2021    

  • Topics in Nutrition Biochemistry and Functional Foods

    2021    

  • General Principles of Applied Biological Chemistry

    2021    

  • Nutrition Biochemistry

    2021    

  • Reviews for Applied Life Sciences

    2021    

  • 応用生命科学研究実験D

    2020   Practical Training  

  • 応用生命科学研究実験C

    2020   Practical Training  

  • 応用生命科学研究実験B

    2020   Practical Training  

  • 応用生命科学研究実験A

    2020   Practical Training  

  • 応用生命科学特別研究実験AII

    2020   Practical Training  

  • 応用生命科学特別研究実験AI

    2020   Practical Training  

  • 応用生命科学プレゼンテーション

    2020    

  • 応用生命科学ゼミナールD

    2020   Practical Training  

  • 応用生命科学ゼミナールC

    2020   Practical Training  

  • 応用生命科学ゼミナールB

    2020   Practical Training  

  • 応用生命科学ゼミナールA

    2020   Practical Training  

  • 科学英語B

    2020   Practical Training  

  • 科学英語A

    2020    

  • 卒業研究

    2020   Practical Training  

  • Basic Biochemistry

    2020    

  • Molecular Cell Biology

    2020    

  • Introduction to Life, Environment, and Advanced Sciences

    2020    

  • General Principles of Applied Biological Chemistry

    2020    

  • Nutrition Biochemistry

    2020    

  • Reviews for Applied Life Sciences

    2020    

▼display all

Charge of off-campus class subject

  • 細胞生物学

  • 栄養生化学

  • 基礎生化学

Faculty development activities

  • FDセミナー  2023

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    「大学院生のキャリアデザイン支援を考える ー文学・理学など基礎系研究分野の支援事例を中心にー」
    報告1:「大学院生等へのキャリア形成支援事業の取組とその成果について」
        大仲 清司・鳥生 隆(教育学修支援室・国際基幹教育機構)
    報告2:「2021年度実施調査から見た大学院進学と修了後進路の状況」
        平 知宏(高等教育研究開発センター)
    質疑・全体討論

  • FDセミナー  2022

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    「内部質保証スタートアップ支援事業成果報告」

  • ワークショップ  2021

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    基調講演「我が国の科学技術政策の最新動向と大学・若手研究者への期待」
    弓場 英司
    (大阪府立大学 大学院工学研究科 准教授、内閣府 科学技術・イノベーション推進事務局 (2020 年度より出向))

  • FDセミナー  2021

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    「THEランキングを通して授業カリキュラム、授業実践を振り返る」
    「国際教養大学の特色(仮)」
    熊谷 嘉隆 氏(国際教養大学副学長)

  • FDセミナー  2021

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    「公立総合大学としての役割と教育のあり方について
      ―新大学大阪公立大学(仮称)で、どのような人間を、どのように育てるか―」
    ■講演:吉川卓治 教授(名古屋大学大学院・教育発達科学研究科)
     「歴史的観点から公立大学の役割と教育のあり方を考える」
    ■コメント:辰巳砂昌弘 大阪府立大学学長(大阪公立大学(仮称)学長就任予定)
     「新大学大阪公立大学(仮称)でどのような人間を、どのように育てるか(仮)」

  • FDセミナー  2021

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    内部質保証スタートアップ支援事業 成果報告会

  • FDセミナー  2020

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    「2020年度第1回工学全体FDセミナー」

  • FDセミナー  2020

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    反転授業実践セミナー
    「工学教育における反転授業の実践事例」

  • FDセミナー  2020

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    FD・SD研修「障がいのある学生を支援するための全学的組織体制」
    村田 淳 先生(京都大学 学生総合支援センター・准教授、)障害学生支援ルーム・チーフコーディネーター

  • FDセミナー  2020

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    応用生命科学類GPSアカデミック結果検討会
    藤井 公雄氏(ベネッセアイキャリア)

  • FDセミナー  2020

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    大学ランキング200 位達成のための研究教育情報戦略
    髙橋雅英 学長補佐(工学研究科 教授)

  • FDセミナー  2020

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    2020年度第2回工学全体FDセミナー
    研究室指導に必要なコーチング技能入門WS
    -学生が自己効力感を高める工夫-

  • FDセミナー  2020

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    第1回「内部質保証スタートアップ支援事業成果報告会」

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Number of papers published by graduate students

  • 2023

    Number of undergraduate student / college student presentations:Number of graduate students presentations:2

  • 2022

    Number of undergraduate student / college student presentations:Number of graduate students presentations:8

  • 2021

    Number of graduate students presentations:2

  • 2020

    Number of graduate students presentations:7

Number of instructed thesis, researches

  • 2023

    Number of instructed the graduation thesis:Number of graduation thesis reviews:7

    [Number of instructed the Master's Program] (previous term):[Number of instructed the Master's Program] (letter term):1

    [Number of master's thesis reviews] (chief):[Number of master's thesis reviews] (vice-chief):8

    [Number of doctoral thesis reviews] (chief):[Number of doctoral thesis reviews] (vice-chief):0

  • 2022

    Number of instructed the graduation thesis:

    [Number of instructed the Master's Program] (previous term):[Number of instructed the Master's Program] (letter term):0

    [Number of master's thesis reviews] (chief):[Number of master's thesis reviews] (vice-chief):6

    [Number of doctoral thesis reviews] (chief):[Number of doctoral thesis reviews] (vice-chief):0

  • 2021

    Number of instructed the graduation thesis:

  • 2020

    Number of instructed the graduation thesis:

Social Activities

  • ロコモ・メタボ予防と医食同源

    2018.04 - 2019.03

  • 模擬講義「西宮市立西宮東高等学校」

    2018.04 - 2019.03

  • 授業体験WEEK 栄養生化学

    2017.04 - 2018.03

  • 出前講義 健康寿命を担う骨格筋~医食同源って何?~

    2015.04 - 2016.03

  • 出前講義 健康寿命を担う骨格筋

    2014.04 - 2015.03

  • 高大連携 高大連携出張講義(兵庫県立柏原高等学校)

    2013.04 - 2014.03

▼display all

Visiting Lectures ⇒ Link to the list of Visiting Lectures

  • 健康寿命を担う骨格筋〜医食同源って何?〜

    Category:Agricultural science (applied biology, biofunctional chemistry, green space environmental science)

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    Audience:High school students, College students, General

    Keyword:骨格筋、加齢、健康寿命、運動、食品成分 

    骨格筋は運動器としてだけでなく、糖代謝を担う主要な組織でもあるので、骨格筋の量と機能を維持・増強することは寝たきりの予防や寝たきりからの早期回復に寄与し、さらに糖尿病のような生活習慣病の予防にもつながります。本講義では、骨格筋形成の分子機構とその分子機構に影響を与える食品成分について紹介します。

Academic Activities

  • Br J Nutr

    Role(s): Peer review

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    Type:Peer review 

  • Biochim Biophys Acta - General Subjects

    Role(s): Peer review

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    Type:Peer review 

  • Cells

    Role(s): Peer review

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    Type:Peer review 

  • Bioscience, Biotechnology, and Biochemistry

    Role(s): Peer review

     More details

    Type:Peer review 

  • ACS Pharmacology & Translational Science

    Role(s): Peer review

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    Type:Peer review 

Foreigner acceptance

  • 2023

    International Students :1

Job title

  • Job title within the department

    School of Agriculture Department of Applied Biological Chemistry 

    学科長  2024.04

  • Job title within the department

    Organization for Research Promotion 生物資源開発センター 

    センター長  2022.04

  • Manager within the university

    Organization for Research Promotion 生物資源開発センター 

    センター長  2021.04 - 2022.03