Updated on 2024/10/08

写真a

 
Ihara Hideshi
 
Organization
Graduate School of Science Department of Biological Chemistry Professor
School of Science Department of Biological Chemistry
Title
Professor
Affiliation
Institute of Science
Affiliation campus
Nakamozu Campus

Position

  • Graduate School of Science Department of Biological Chemistry 

    Professor  2022.04 - Now

  • School of Science Department of Biological Chemistry 

    Professor  2022.04 - Now

Degree

  • 博士(農学) ( Others )

Research Interests

  • Reactive sulfur species

  • レドックスシグナル

  • 一酸化窒素

  • 活性酸素

  • イミダゾールジペプチド

  • グリア細胞

  • 神経細胞

Research subject summary

  • イミダゾールジペプチドのレドックス修飾代謝物

  • 活性イオウ分子種による生理機能調節

Committee Memberships (off-campus)

  • 代議員   日本酸化ストレス学会  

    2020.05 - Now 

Papers

  • Development of methods for quantitative determination of the total and reactive polysulfides: Reactive polysulfide profiling in vegetables

    Kasamatsu S.

    Food Chemistry   413   135610   2023.07( ISSN:03088146

  • The Sulfide-Responsive SqrR/BigR Homologous Regulator YgaV of Escherichia coli Controls Expression of Anaerobic Respiratory Genes and Antibiotic Tolerance

    Balasubramanian R.

    Antioxidants   11 ( 12 )   2022.12( ISSN:2076-3921

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  • Quantitative Determination of 2-Oxo-Imidazole-Containing Dipeptides by High-Performance Liquid Chromatography/Tandem Mass Spectrometry

    Komae S.

    Antioxidants   11 ( 12 )   2022.12( ISSN:2076-3921

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  • 2-Oxo-imidazole dipeptides in meat

    Komae S.

    Bioscience, biotechnology, and biochemistry   86 ( 11 )   1576 - 1580   2022.10( ISSN:0916-8451

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  • S-allyl cysteine increases blood flow in NO-dependent and -independent manners

    Takemura Shigekazu, Ihara Hideshi, Nakagawa Kanako, Minamiyama Yukiko

    Glycative Stress Research   9 ( 3 )   146 - 157   2022.09( ISSN:21883602 ( eISSN:21883610

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    S-allylcysteine (SAC) is a bioactive substance found in aged garlic and is known to improve liver function, inhibit organ fibrosis, and have anticancer activity. A single oral administration of SAC (10 mg/kg) increased tail vein blood flow in rats by up to 10% over one hour, and a 0.3% SAC mixed diet increased tail vein blood flow compared to the normal diet after 2 months. To investigate the relationship between these increases in blood flow and nitric oxide (NO), a single oral dose of SAC (100 mg/kg) followed by a 3-day administration of L-nitroarginine (LNA, 2 mM), an NO synthase inhibitor, resulted in the disappearance of SAC-induced increases in blood flow. Whereas, when LNA and excess L-arginine were mixed with drinking water, the SAC-induced increase in blood flow was restored. This phenomenon was also observed in plantar blood flow. These results indicate that the increase in blood flow induced by SAC is NO-dependent. To investigate the relationship between these increases in blood flow and NO, a single oral dose of SAC (100 mg/kg) followed by a 3-day administration of LNA (2 mM), resulted in the disappearance of SAC-induced increases in blood flow. Whereas, when LNA and excess L-arginine were mixed with drinking water, the SAC-induced increase in blood flow was restored. This phenomenon was also observed in plantar blood flow. These results indicate that the increase in blood flow by SAC is NO-dependent. Next, Western blotting of vasodilator-stimulated phosphoprotein (VASP) was performed to confirm this in a vascular model. Phosphorylated VASP (pVASP) is characterized as a substrate for both cGMP-dependent and cAMP-dependent kinases. LNA abolished the aortic pVASP band, whereas LNA plus SAC partially recovered. Focusing on the NO-independent vasodilator H2S, measurements of plasma reactive persulfide showed that LNA treatment significantly reduced thiols, i.e., cysteine, cystine, glutathione (GSH), and H2S. Of the thiols reduced by LNA treatment, the concurrent SAC application significantly restored the reduction of cysteine and cystine, but not H2S. These results suggest that SAC may maintain nitrosothiols (compounds of nitric oxide and thiols) that act as NO donors and indirectly increase the bioavailability of NO, resulting in blood flow augmentation through its antioxidant capacity by free radical scavenging.

    DOI: 10.24659/gsr.9.3_146

  • S-allyl cysteine increases blood flow in NO-dependent and-independent manners(和訳中)

    Takemura Shigekazu, Ihara Hideshi, Nakagawa Kanako, Minamiyama Yukiko

    Glycative Stress Research   9 ( 3 )   146 - 157   2022.09( ISSN:2188-3602

  • 2-Oxo-Imidazole-Containing Dipeptides Play a Key Role in the Antioxidant Capacity of Imidazole-Containing Dipeptides Reviewed

    Kasamatsu, Shingo Komae, Somei Matsukura, Kana Kakihana, Yuki Uchida, Koji Ihara, Hideshi

    Antioxidants 雑誌   10 ( 9 )   2021.09

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    Kind of work:Joint Work  

  • Sulfide catabolism ameliorates hypoxic brain injury Reviewed

    Marutani, E. Morita, M. Hirai, S. Kai, S. Grange, R. M. H. Miyazaki, Y. Nagashima, F. Traeger, L. Magliocca, A. Ida, T. Matsunaga, T. Flicker, D. R. Corman, B. Mori, N. Yamazaki, Y. Batten, A. Li, R. Tanaka, T. Ikeda, T. Nakagawa, A. Atochin, D. N. Ihara, H. Olenchock, B. A. Shen, X. Nishida, M. Hanaoka, K. Kevil, C. G. Xian, M. Bloch, D. B. Akaike, T. Hindle, A. G. Motohashi, H. Ichinose, F.

    Nature communications 雑誌   12 ( 1 )   2021.05

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    Kind of work:Joint Work  

  • High Precision Sulfur Metabolomics Innovated by a New Specific Probe for Trapping Reactive Sulfur Species Reviewed

    Kasamatsu, S. Ida, T. Koga, T. Asada, K. Motohashi, H. Ihara, H. Akaike, T.

    Antioxid Redox Signal 雑誌   34 ( 18 )   2021.05

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    Kind of work:Joint Work  

  • Distribution and quantitative analysis of homoanserine and its 2-oxo derivative in mouse tissues Reviewed

    Kakihana Y, Kasamatsu S, Uchida K, Ihara H

    Free Radic Res 雑誌   2021.01

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  • Generation of Rat Monoclonal Antibody to Detect Hydrogen Sulfide and Polysulfides in Biological Samples Reviewed

    Kasamatsu, S. Kakihana, Y. Koga, T. Yoshioka, H. Ihara, H.

    Antioxidants (Basel) 雑誌   9 ( 11 )   2020.11

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    Kind of work:Joint Work  

  • High Precision Sulfur Metabolomics Innovated by a New Specific Probe for Trapping Reactive Sulfur Species Reviewed

    Kasamatsu, S. Ida, T. Koga, T. Asada, K. Motohashi, H. Ihara, H. Akaike, T.

    Antioxid Redox Signal 雑誌   2020.11

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    Kind of work:Joint Work  

  • Enzymatic Regulation and Biological Functions of Reactive Cysteine Persulfides and Polysulfides Reviewed

    Sawa, Tomohiro Motohashi, Hozumi Ihara, Hideshi Akaike, Takaaki

    Biomolecules 雑誌   10 ( 9 )   2020.08

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  • 8-Nitro-cGMP modulates exocytosis in adrenal chromaffin cells Reviewed

    Tsutsuki, H. Kasamatsu, S. Kunieda, K. Ida, T. Sawa, T. Sasakawa, N. Akaike, T. Ihara, H.

    Biochem Biophys Res Commun 雑誌   526 ( 1 )   2020.03

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  • Enhanced Cellular Polysulfides Negatively Regulate TLR4 Signaling and Mitigate Lethal Endotoxin Shock Reviewed

    Zhang, T., Ono, K., Tsutsuki, H., Ihara, H., Islam, W., Akaike, T., and Sawa, T.

    Cell Chem Biol 雑誌   26   686 - 698   2019.05

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  • 8-Nitro-cGMP attenuates context-dependent fear memory in mice

    Kishimoto Y.

    Biochemical and Biophysical Research Communications   511 ( 1 )   141 - 147   2019.03( ISSN:0006291X

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  • Polysulfide stabilization by tyrosine and hydroxyphenyl-containing derivatives that is important for a reactive sulfur metabolomics analysis Reviewed

    Hamid, H. A., Tanaka, A., Ida, T., Nishimura, A., Matsunaga, T., Fujii, S., Morita, M., Sawa, T., Fukuto, J. M., Nagy, P., Tsutsumi, R., Motohashi, H., Ihara, H., and Akaike, T.

    Redox Biology 雑誌   21   2019.02

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  • 8-Nitro-cGMP attenuates context-dependent fear memory in mice Reviewed

    Kishimoto, Y. Kasamatsu, S. Yanai, S. Endo, S. Akaike, T. Ihara, H.

    Biochem Biophys Res Commun 雑誌   511 ( 1 )   2019.02

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  • Redox regulation of Ca2+/calmodulin-dependent protein kinase IV via oxidation of its active-site cysteine residue Reviewed

    Takata, T., Kimura, J., Ihara, H., Hatano, N., Tsuchiya, Y., and Watanabe, Y.

    Free Radic. Biol. Med. 雑誌   130   99 - 106   2019.01

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  • 2-Oxo-histidine-containing dipeptides are functional oxidation products Reviewed

    Ihara, H., Kakihana, Y., Yamakage, A., Kai, K., Shibata, T., Nishida, M., Yamada, K., and Uchida, K.

    J. Biol. Chem. 雑誌   294   1279 - 1289   2019.01

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  • SNAP-25 S-Guanylation and SNARE Complex Formation Reviewed

    Kishimoto, Y., Akaike, T., and Ihara, H.

    Methods Mol. Biol. 雑誌   1860   163 - 173   2019.01

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  • Involvement of nitric oxide/reactive oxygen species signaling via 8-nitro-cGMP formation in 1-methyl-4-phenylpyridinium ion-induced neurotoxicity in PC12 cells and rat cerebellar granule neurons Reviewed

    Masuda, K., Tsutsuki, H., Kasamatsu, S., Ida, T., Takata, T., Sugiura, K., Nishida, M., Watanabe, Y., Sawa, T., Akaike, T., and Ihara, H.

    Biochem. Biophys. Res. Commun. 雑誌   495   2165 - 2170   2018.01

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  • 8-Nitro-cGMP Attenuates the Interaction between SNARE Complex and Complexin through S-Guanylation of SNAP-25 Reviewed

    Kishimoto, Y., Kunieda, K., Kitamura, A., Kakihana, Y., Akaike, T., and Ihara, H.

    ACS Chem. Neurosci. 雑誌   9   217 - 223   2017.11

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  • Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics Reviewed

    Akaike, T., Ida, T., Wei, F. Y., Nishida, M., Kumagai, Y., Alam, M. M., Ihara, H., Sawa, T., Matsunaga, T., Kasamatsu, S., Nishimura, A., Morita, M., Tomizawa, K., Nishimura, A., Watanabe, S., Inaba, K., Shima, H., Tanuma, N., Jung, M., Fujii, S., Watanabe, Y., Ohmuraya, M., Nagy, P., Feelisch, M., Fukuto, J. M., and Motohashi, H

    Nat Commun 雑誌   8   2017.10

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  • 8-Nitro-cGMP promotes bone growth through expansion of growth plate cartilage Reviewed

    Hoshino, M., Kaneko, K., Miyamoto, Y., Yoshimura, K., Suzuki, D., Akaike, T., Sawa, T., Ida, T., Fujii, S., Ihara, H., Tanaka, J., Tsukuura, R., Chikazu, D., Mishima, K., Baba, K., and Kamijo, R.

    Free Radic. Biol. Med. 雑誌   110   63 - 71   2017.09

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  • Ferulic acid and its water-soluble derivatives inhibit nitric oxide production and inducible nitric oxide synthase expression in rat primary astrocytes Reviewed

    Kikugawa, M., Ida, T., Ihara, H., and Sakamoto, T.

    Biosci. Biotechnol. Biochem. 雑誌   81   1607 - 1611   2017.08

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  • Exposure to Electrophiles Impairs Reactive Persulfide-dependent Redox Signaling in Neuronal Cells Reviewed

    Ihara, H., Kasamatsu, S., Kitamura, A., Nishimura, A., Tsutsuki, H., Ida, T., Ishizaki, K., Toyama, T., Yoshida, E., Abdul Hamid, H., Jung, M., Matsunaga, T., Fujii, S., Sawa, T., Nishida, M., Kumagai, Y., and Akaike, T.

    Chem. Res. Toxicol. 雑誌   30   1673 - 1684   2017.08

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  • Reactive sulfur species inactivate Ca2+/calmodulin-dependent protein kinase IV via S-polysulfidation of its active-site cysteine residue Reviewed

    Takata, T., Ihara, H., Hatano, N., Tsuchiya, Y., Akaike, T., and Watanabe, Y.

    Biochem. J. 雑誌   474   2547 - 2562   2017.07

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  • Synthesis of L-cysteine derivatives containing stable sulfur isotopes and application of this synthesis to reactive sulfur metabolome Reviewed

    Ono, K., Jung, M., Zhang, T., Tsutsuki, H., Sezaki, H., Ihara, H., Wei, F. Y., Tomizawa, K., Akaike, T., and Sawa, T.

    Free Radic. Biol. Med. 雑誌   106   69 - 79   2017.05

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  • Quantitative determination of polysulfide in albumins, plasma proteins and biological fluid samples using a novel combined assays approach Reviewed

    Ikeda, M., Ishima, Y., Shibata, A., Chuang, V. T. G., Sawa, T., Ihara, H., Watanabe, H., Xian, M., Ouchi, Y., Shimizu, T., Ando, H., Ukawa, M., Ishida, T., Akaike, T., Otagiri, M., and Maruyama, T.

    Anal. Chim. Acta 雑誌   969   18 - 25   2017.05

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  • Superoxide generation from nNOS splice variants and its potential involvement in redox signal regulation Reviewed

    Ihara, H., Kitamura, A., Kasamatsu, S., Ida, T., Kakihana, Y., Tsutsuki, H., Sawa, T., Watanabe, Y., and Akaike, T.

    Biochem. J. 雑誌   474   1149 - 1162   2017.03

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  • Endogenous occurrence of protein S-guanylation in Escherichia coli: Target identification and genetic regulation Reviewed

    Tsutsuki, H., Jung, M., Zhang, T., Ono, K., Ida, T., Kunieda, K., Ihara, H., Akaike, T., and Sawa, T.

    Biochem. Biophys. Res. Commun. 雑誌   478   7 - 11   2016.09

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  • Optimization of a Neurotoxin to Investigate the Contribution of Excitatory Interneurons to Speed Modulation In Vivo Reviewed

    Sternberg, J. R., Severi, K. E., Fidelin, K., Gomez, J., Ihara, H., Alcheikh, Y., Hubbard, J. M., Kawakami, K., Suster, M., and Wyart, C.

    Curr. Biol. 雑誌   26   2319 - 2328   2016.09

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  • Naringin lauroyl ester inhibits lipopolysaccharide-induced activation of nuclear factor κB signaling in macrophages Reviewed

    Hattori, H., Tsutsuki, H., Nakazawa, M., Ueda, M., Ihara, H., and Sakamoto, T.

    Biosci. Biotechnol. Biochem. 雑誌   80   1403 - 1409   2016.07

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  • Redox signaling regulated by an electrophilic cyclic nucleotide and reactive cysteine persulfides Reviewed

    Fujii, S., Sawa, T., Nishida, M., Ihara, H., Ida, T., Motohashi, H., and Akaike, T.

    Arch. Biochem. Biophys. 雑誌   595   140 - 146   2016.04

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  • Redox signaling regulated by electrophiles and reactive sulfur species Reviewed

    Nishida, M., Kumagai, Y., Ihara, H., Fujii, S., Motohashi, H., and Akaike, T.

    J. Clin. Biochem. Nutr. 雑誌   58   91 - 98   2016.03

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  • Water-soluble ferulic acid derivatives improve amyloid-β-induced neuronal cell death and dysmnesia through inhibition of amyloid-β aggregation Reviewed

    Kikugawa, M., Tsutsuki, H., Ida, T., Nakajima, H., Ihara, H., and Sakamoto, T.

    Biosci. Biotechnol. Biochem. 雑誌   80   547 - 553   2016.03

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    Kind of work:Joint Work  

  • Formation of Sulfur Adducts of N-Acetyl-p-benzoquinoneimine, an Electrophilic Metabolite of Acetaminophen in Vivo: Participation of Reactive Persulfides Reviewed

    Abiko, Y., Ishii, I., Kamata, S., Tsuchiya, Y., Watanabe, Y., Ihara, H., Akaike, T., and Kumagai, Y.

    Chem. Res. Toxicol. 雑誌   28   1796 - 1802   2015.08

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  • 8-Nitro-cGMP Enhances SNARE Complex Formation through S-Guanylation of Cys90 in SNAP25 Reviewed

    Kunieda, K., Tsutsuki, H., Ida, T., Kishimoto, Y., Kasamatsu, S., Sawa, T., Goshima, N., Itakura, M., Takahashi, M., Akaike, T., and Ihara, H.

    ACS Chem. Neurosci. 雑誌   6   1715 - 1725   2015.07

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  • 8-Mercapto-Cyclic GMP Mediates Hydrogen Sulfide-Induced Stomatal Closure in Arabidopsis Reviewed

    Honda, K., Yamada, N., Yoshida, R., Ihara, H., Sawa, T., Akaike, T., and Iwai, S.

    Plant Cell Physiol. 雑誌   56   1481 - 1489   2015.05

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  • Nuclear-translocated glyceraldehyde-3-phosphate dehydrogenase promotes Poly(ADP-ribose) polymerase-1 activation during oxidative/nitrosative stress in stroke Reviewed

    Nakajima, H., Kubo, T., Ihara, H., Hikida, T., Danjo, T., Nakatsuji, M., Shahani, N., Itakura, M., Ono, Y., Azuma, Y. T., Inui, T., Kamiya, A., Sawa, A., and Takeuchi, T.

    J. Biol. Chem. 雑誌   290   14493 - 14503   2015.04

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  • Reactive cysteine persulfides and S-polythiolation regulate oxidative stress and redox signaling Reviewed

    Ida, T., Sawa, T., Ihara, H., Tsuchiya, Y., Watanabe, Y., Kumagai, Y., Suematsu, M., Motohashi, H., Fujii, S., Matsunaga, T., Yamamoto, M., Ono, K., Devarie-Baez, N. O., Xian, M., Fukuto, J. M., and Akaike, T.

    Proc. Natl. Acad. Sci. U. S. A. 雑誌   111   7606 - 7611   2014.05

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  • Redox signal regulation via nNOS phosphorylation at Ser847in PC12 cells and rat cerebellar granule neurons Reviewed

    Kasamatsu, S., Watanabe, Y., Sawa, T., Akaike, T., and Ihara, H. (2014)

    Biochem. J. 雑誌   459   107 - 116   2014.04

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  • Theophylline Potentiates Lipopolysaccharide-iduced NO Production in Cultured Astrocytes Reviewed

    Ogawa, M. Takano, K. Kawabe, K. Moriyama, M. Ihara, H. Nakamura, Y. 

    Neurochem. Res. 雑誌   39 ( 1 )   107 - 116   2014.01

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  • Nitric oxide enhances increase in cytosolic Ca2+ and promotes nicotine- triggered MAPK pathway in PC12 cells

     

    Nitric Oxide   34 ( 1 )   2013.11

  • Formation, signaling functions, and metabolisms of nitrated cyclic nucleotide.

     

    Nitric Oxide   34 ( 1 )   2013.11

  • Substrate specificity and gene expression of two Penicillium chrysogenum α-L: -arabinofuranosidases (AFQ1 and AFS1) belonging to glycoside hydrolase families 51 and 54 Reviewed

    Sakamoto T, Inui M, Yasui K, Hosokawa S, Ihara H

    Appl Microbiol Biotechnol. 雑誌   97 ( 3 )   1121 - 30   2013.02

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  • Studies on fish scale collagen of pacific saury (Cololabis saira) Reviewed

    Mori H, Tone Y, Shimizu K, Zikihara K, Tokutomi S, Ida T, Ihara H, Hara M

    Materials Science and Engineering C 雑誌   33 ( 1 )   174 - 81   2013.01

  • Subtilase cytotoxin enhances E. coli survival in macrophages by suppression of nitric oxide production through the inhibition of NF-κB activation Reviewed

    Tsutsuki H, Yahiro K, Suzuki K, Suto A, Ogura K, Nagasawa S, Ihara H, Shimizu T, Nakajima H, Moss J, Noda M

    Infect. Immun. 雑誌   80 ( 11 )   3939 - 51   2012.11

  • Inhibition of Nitric Oxide Production and Inducible Nitric Oxide Synthase Expression by a Polymethoxyflavone from Young Fruits of Citrus unshiu in Rat Primary Astrocytes Reviewed

    IHARA H, YAMAMOTO H, IDA T,TSUTSUKI H, SAKAMOTO T,FUJITA T,OKADA T, KOZAKI S

    Biosci Biotechnol Biochem. 雑誌   76 ( 10 )   1843 - 8   2012.10

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  • Hydrogen sulfide anion regulates redox signaling via electrophile sulfhydration. Reviewed

    Nishida M, Sawa T, Kitajima N, Ono K, Inoue H, Ihara H, Motohashi H, Yamamoto M, Suematsu M, Kurose H, van der Vliet A, Freeman BA, Shibata T, Uchida K, Kumagai Y, Akaike T.

    Nature Chemical Biology 雑誌   8   714 - 24   2012.08

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  • Induction of Apoptosis in MCF-7 Cells by β-1,3-Xylooligosaccharides Prepared from Caulerpa lentillifera. Reviewed

    Maeda R, Ida T, Ihara H, Sakamoto T.

    Biosci Biotechnol Biochem. 雑誌   76 ( 5 )   1032 - 4   2012.05

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  • Specificity of botulinum protease for human VAMP family proteins. Reviewed

    Yamamoto H, Ida T, Tsutsuki H, Mori M, Matsumoto T, Kohda T, Mukamoto M, Goshima N, Kozaki S, Ihara H

    Microbiol Immunol. 雑誌   56 ( 4 )   245 - 53   2012.04

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  • Immunostimulatory Activity of Polysaccharides Isolated from Caulerpa lentillifera on Macrophage Cells. Reviewed

    Maeda R, Ida T, Ihara H, Sakamoto T.

    Biosci Biotechnol Biochem. 雑誌   76 ( 3 )   501 - 5   2012.03

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  • Regulation by mitochondrial superoxide and NADPH oxidase of cellular formation of nitrated cyclic GMP: potential implications for ROS signaling. Reviewed

    Ahmed KA, Sawa T, Ihara H, Kasamatsu S, Yoshitake J, Rahaman MM, Okamoto T, Fujii S, Akaike T.

    Biochem. J. 雑誌   441 ( 2 )   719 - 30   2012.01

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  • Nitric oxide promotes nicotine-triggered ERK signaling via redox reactions in PC12 cells Reviewed

    Miyamoto Y, Sakai R, Maeda C, Takata T, Ihara H, Tsuchiya Y, Watanabe Y.

    Nitric Oxide 雑誌   25 ( 3 )   344 - 9   2011.10

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  • Methodological proof of immunochemistry for specific identification of 8-nitroguanosine 3´,5´-cyclic monophosphate formed in glia cells Reviewed

    Ihara H, Ahtesham AK, Ida T, Kasamatsu S, Kunieda K, Okamoto T, Sawa T, Akaike T.

    Nitric Oxide 雑誌   25 ( 2 )   169 - 75   2011.08

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  • Identification of a GH62 α-L: -arabinofuranosidase specific for arabinoxylan produced by Penicillium chrysogenum Reviewed

    Sakamoto T, Ogura A, Inui M, Tokuda S, Hosokawa S, Ihara H, Kasai N

    Appl. Microbiol. Biotechnol. 雑誌   90 ( 1 )   137 - 46   2011.04

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  • Antioxidant effect of a nitrated cyclic nucleotide functioning as an endogenous electrophile Reviewed

    Sawa T, Ihara H, Akaike T

    Curr. Top. Med. Chem. 雑誌   11 ( 14 )   1854 - 1860   2011.04

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  • Characterization of Trichoderma polysporum from Spitsbergen, Svalbard archipelago, Norway, with species identity, pathogenicity to moss, and polygalacturonase activity Reviewed

    Yamazaki Y, Tojo M, Hoshino T, Kida K, Sakamoto T, Ihara H, Yumoto I, Tronsmo AM, Kanda H

    Fungal Ecol. 雑誌   4 ( 1 )   15 - 21   2011.02

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  • PKC-Dependent Inhibition of Ca2+-Dependent Exocytosis from Astrocytes Reviewed

    Yasuda K, Itakura M, Aoyagi K, Sugaya T, Nagata E, Ihara H, Takahashi M

    Glia 雑誌   59 ( 1 )   143 - 51   2011.01

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  • Nucleotides function as endogenous chemical sensors for oxidative stress signaling Reviewed

    Ihara H, Sawa T, Nakabeppu Y, Akaike T

    J. Clin. Biochem. Nutr. 雑誌   48 ( 1 )   2011.01

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  • Cell signaling mediated by nitrated cyclic guanine nucleotide Reviewed

    Akaike T, Fujii S, Sawa T, Ihara H.

    Nitric Oxide 雑誌   23 ( 3 )   166 - 174   2010.11

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  • The critical role of nitric oxide signaling, via protein S-guanylation and nitrated cyclic GMP, in the antioxidant adaptive response. Reviewed

    第一著者Fujii S, Sawa T, Ihara H, Tong KI, Ida T, Okamoto T, Ahtesham AK, Ishima Y, Motohashi H, Yamamoto M, Akaike T.

    J. Biol. Chem. 雑誌   285 ( 31 )   23970 - 84   2010.07

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    Kind of work:Joint Work  

  • Effects of low protein intake on the development of the remaining kidney in subtotally nephrectomized immature rats: expression of inducible and endothelial NO synthase. Reviewed

    Mino M, Ihara H, Kozaki S, Kondo T, Takeshita A, Kusakabe KT, Okada T.

    Med. Mol. Morphol. 雑誌   43 ( 2 )   116 - 122   2010.06

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    Kind of work:Joint Work  

  • Identification of two GH27 bifunctional proteins with beta-L-arabinopyranosidase/alpha-D-galactopyranosidase activities from Fusarium oxysporum. Reviewed

    Sakamoto T, Tsujitani Y, Fukamachi K, Taniguchi Y, Ihara H.

    Appl Environ Microbiol. 雑誌   86 ( 4 )   1115 - 24   2010.04

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    Kind of work:Joint Work  

  • Glyceraldehyde-3-phosphate dehydrogenase aggregate formation participates in oxidative stress-induced cell death. Reviewed

    Nakajima H, Amano W, Kubo T, Fukuhara A, Ihara H, Azuma YT, Tajima H, Inui T, Sawa A, Takeuchi T

    J. Biol. Chem. 雑誌   284 ( 49 )   34331 - 41   2009.12

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    Kind of work:Joint Work  

  • Characteristics of enterotoxin H-producing Staphylococcus aureus isolated from clinical cases and properties of the enterotoxin productivity Reviewed

    Sakai F, Ihara H, Aoyama K, Igarashi H, Yanahira S, Ohkubo T, Asao T, Kozaki S

    J Food Prot 雑誌   71 ( 9 )   1855 - 1860   2008.09

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    Kind of work:Joint Work  

  • Identification of the receptor-binding sites in the carboxyl-terminal half of the heavy chain of botulinum neurotoxin types C and D. Reviewed

    Tsukamoto K, Kozai Y, Ihara H, Kohda T, Mukamoto M, Tsuji T, Kozaki S.

    Microb Pathog 雑誌   44 ( 6 )   484 - 493   2008.06

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    Kind of work:Joint Work  

  • Nitric oxide-mediated modulation of calcium/calmodulin-dependent protein kinase II. Reviewed

    Song T, Hatano N, Kambe T, Miyamoto Y, Ihara H, Yamamoto H, Sugimoto K, Kume K, Yamaguchi F, Tokuda M, Watanabe Y.

    Biochemical Journal 雑誌   412 ( 2 )   223 - 231   2008.06

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    Kind of work:Joint Work  

  • Study of the irreversible binding of Bacillus thuringiensis CryIAa to brush border membrane vesicles from Bombyx mori midgut Reviewed

    Ihara H, Himeno M

    Journal of Invertebrate Pathology 雑誌   98 ( 2 )   177 - 183   2008.06

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    Kind of work:Joint Work  

  • Cellular responses in the spinal cord during development of hypomyelination in the mv rat. Reviewed

    Izawa T, Takenaka S, Ihara H, Kotani T, Yamate J, Franklin RJ, Kuwamura M.

    Brain Research 雑誌   21 ( 1195C )   120 - 129   2008.02

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    Kind of work:Joint Work  

  • Cytokine-induced enhancement of calcium-dependent glutamate release from astrocytes mediated by nitric oxide Reviewed

    責任著者Ida T, Hara M, Nakamura Y, Kozaki S, Tsunoda S, Ihara H.

    Neuroscience letters 雑誌   432 ( 3 )   232 - 236   2008.02

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    Kind of work:Joint Work  

  • Aspartyl-tRNA synthetases from two halophilic archae Halobacterium salinarum and Haloferax volcanii Reviewed

    Ihara H, Shinoda H, Yamaji R, Inui H, Miyatake K, Wadano A, Nakano Y, Ishigami M

    Applied Biological Science 雑誌   9   1 - 14   2008.01

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    Kind of work:Joint Work  

  • Protein S-guanylation by the biological signal 8-nitroguanosine 3',5'-cyclic monophosphate Reviewed

    Sawa T, Zaki MH, Okamoto T, Akuta T, Tokutomi T, Kim-Mitsuyama S, Ihara H, Kobayashi A, Yamamoto M, Fujii S, Arimoto H, Akaike T

    Nature Chemical Biology 雑誌   3 ( 11 )   727 - 735   2007.11

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    Kind of work:Joint Work  

  • Alternative polyadenylation sites of human endothelial nitric oxide synthase mRNA Reviewed

    Ihara H, Tsutsuki H, Ida T, Kozaki S, Tsuyama S, Moss J

    Biochemical and Biophysical Research Communications 雑誌   363   146 - 152   2007.08

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    Kind of work:Joint Work  

  • Characterization of {beta}-1,6-galactanase of Fusarium oxysporum, an enzyme that hydrolyzes larch wood arabinogalactan. Reviewed

    Sakamoto T, Taniguchi Y, Suzuki S, Ihara H, Kawasaki H.

    Appl Environ Microbiol. 雑誌   73 ( 9 )   3109 - 12   2007.05

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    Kind of work:Joint Work  

  • Nitric oxide inhibits depolarization-evoked glutamate release from rat cerebellar granule cells Reviewed

    責任著者Tsutsuki H, Kohda T, Hara M, Kozaki S, Ihara H

    Nitric Oxide 雑誌   16 ( 2 )   217 - 227   2007.03

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    Kind of work:Joint Work  

  • Differential contribution of the residues in C-terminal half of heavy chain of botulinum neurotoxin type B to its binding to ganglioside and synaptotagmin 2/ganglioside Reviewed

    Kohda T, Ihara H, Seto Y, Nakaya T, Tsustsuki H, Mukamoto M, Kozaki

    Microbial Pathogenesis 雑誌   42 ( 2-3 )   72 - 79   2007.02

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    Kind of work:Joint Work  

  • P90 RSK-1 associates with and inhibits neuronal nitric-oxide synthase Reviewed

    Song T, Sugimoto K, Ihara H, Mizutani A, Hatano N, Kume K, Kambe T, Yamaguchi F, Tokuda M, Watanabe Y

    Biochemical Journal 雑誌   401 ( 2 )   391 - 398   2007.01

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    Kind of work:Joint Work  

  • Expression of neuronal nitric oxide synthase variant, nNOS-m, in rat brain Reviewed

    Ihara H, Kuwamura M, Atsuta M, Nihonmatsu I, Okada T, Mukamoto M, Kozaki S

    Nitric Oxide 雑誌   15 ( 1 )   13 - 19   2006.08

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    Kind of work:Joint Work  

  • Expression of foreign type I ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39) stimulates photosynthesis in cyanobacterium Synechococcus PCC7942 cells Reviewed

    Iwaki T, Haranoh K, Inoue N, Kojima K, Satoh R, Nishino T, Wada S, Ihara H, Tsuyama S, Kobayashi H, Wadano A,

    Photosynthesis Research 雑誌   88 ( 3 )   287 - 297   2006.06

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    Kind of work:Joint Work  

  • Potentiation by high potassium of lipopolysaccharide-induced nitric oxide production from cultured astrocytes Reviewed

    Nakamura Y, Kitagawa T, Ihara H, Kozaki S, Moriyama M, Kannan Y

    Neurochemistry International 雑誌   48 ( 1 )   43 - 49   2006.01

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    Kind of work:Joint Work  

  • Binding of Clostridium botulinum type C and D neurotoxins to ganglioside and phospholipid. Novel insights into the receptor for clostridial neurotoxins Reviewed

    Tsukamoto K, Kohda T, Mukamoto M, Takeuchi K, Ihara H, Saito M, Kozaki S

    J. Biol. Chem. 雑誌   280 ( 42 )   35164 - 35171   2005.10

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    Kind of work:Joint Work  

  • Molecular identification of a cold-adapated endo-arabinanase of Penicillium chrysogenum Reviewed

    第一著者Sakamoto T, Ihara H, Shibano A, Nagahiro H, Kawasaki H

    J. Appl. Glycosci. 雑誌   52 ( 4 )   369 - 372   2005.10

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    Kind of work:Joint Work  

  • A novel neurotoxoid vaccine prevents mucosal botulism Reviewed

    Kobayashi R, Kohda T, Kataoka K, Ihara H, Kozaki S, Pascual DW, Staats HF, Kiyono H, McGhee JR, Fujihashi K.

    Journal of Immunlogy 雑誌   174 ( 4 )   2190 - 2195   2005.02

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    Kind of work:Joint Work  

  • Differential effects of interferon-gamma on production of trypanosome-derived lymphocyte-triggering factor by Trypanosoma brucei gambiense and Trypanosoma brucei brucei Reviewed

    Nishimura K, Hamashita K, Okamoto Y, Kawahara F, Ihara H, Kozaki S, Ohnishi Y, Yamasaki S

    JOURNAL OF PARASITOLOGY 雑誌   90 ( 4 )   740 - 745   2004.08

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    Kind of work:Joint Work  

  • Molecular characterization of a Penicillium chrysogenum exo-1,5-alpha-L-arabinanase that is structurally distinct from other arabinan-degrading enzymes Reviewed

    第一著者Sakamoto T, Ihara H, Shibano A, Kasai N, Inui H, Kawasaki H

    FEBS LETTERS 雑誌   560 ( 1-3 )   199 - 204   2004.02

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    Kind of work:Joint Work  

  • A cold-adapted endo-arabinanase from Penicillium chrysogenum Reviewed

    Sakamoto T, Ihara H, Kozaki S, Kawasaki H

    BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 雑誌   1624 ( 1-3 )   740 - 745   2003.12

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    Kind of work:Joint Work  

  • Sequence of the gene for Clostridium botulinum type B neurotoxin associated with infant botulism, expression of the C-terminal half of heavy chain and its binding activity Reviewed

    Ihara H, Kohda T, Morimoto F, Tsukamoto K, Karasawa T, Nakamura S, Mukamoto M, Kozaki S

    BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION 雑誌   1625 ( 1 )   19 - 26   2003.01

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    Kind of work:Joint Work  

  • Characterization of Clostridium butyricum neurotoxin associated with food-borne botulism Reviewed

    Tsukamoto K, Mukamoto M, Kohda T, Ihara H, Wang XM, Maegawa T, Nakamura S, Karasawa T, Kozaki S

    MICROBIAL PATHOGENESIS 雑誌   33 ( 4 )   177 - 184   2002.10

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  • Characterization of a novel monoclonal antibody that senses nitric oxide-dependent activation of soluble guanylate cyclase Reviewed

    Shingo Tsuyama, Eri Yamazaki, Takeshi Tomita, Hideshi Ihara, Shigeo Takenaka, Keiko Kato, Shunji Kozaki

    FEBS Lett 雑誌   455 ( 3 )   291 - 294   1999.07

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    Kind of work:Joint Work  

  • Endothelial nitric oxide synthase as a potential susceptibility gene in the pathogenesis of emphysema in alpha-antitrypsin deficiency Reviewed

    Alexy Novoradovsky, Mark L. Brantly, Myron A. Waclawiw, Prerna P. Chaudhary, Hideshi Ihara, Lin Qi, N. Tony Eissa, Pauline M. Barnes, Kateri M. Gabriele, Mary E. Ehrmantraut, Paola Rogliani, Joel Moss

    Am. J. Respir. Cell Mol. Biol. 雑誌   20 ( 3 )   441 - 447   1999.05

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    Kind of work:Joint Work  

  • Purification and partial amino acid sequences of the binding protein from Bombyx mori for CryIAa ?-endotoxin of Bacillus thuringiensis Reviewed

    H. Ihara, T. Uemura, M. Masuhara, S. Ikawa, K. Sugimoto, A. Wadano, M. Himeno

    Comp. Biochem. Physiol. 雑誌   120B ( 1 )   9197 - 9204   1998.05

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    Kind of work:Joint Work  

  • Molecular evolution of aspartyl tRNA synthetases and origin of universal genetic code and urkingdoms of living things Reviewed

    M. Ishigami, H. Ihara, H. Shinoda

    Viva Origino 雑誌   25   73 - 86   1997.11

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    Kind of work:Joint Work  

  • Molecular evolution of aminoacyl tRNA synthetases and origin of universal genetic code Reviewed

    M. Ishigami, H. Ihara, H. Shinoda

    Proceedings of the 5th International Conference on Bioastronomy, 雑誌   483 - 489   1997.07

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    Kind of work:Joint Work  

  • Three extracellular chitinases in suspension-cultured rice cells elicited by N-acetylchitooligosaccharides Reviewed

    H. Inui, Y. Yamaguchi, Yo. Ishigami, S. Kawaguchi, T. Yamada, H. Ihara, S. Hirano

    Biosci. Biotech. Biochem. 雑誌   60 ( 12 )   1956 - 1961   1996.12

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    Kind of work:Joint Work  

  • Mode of action of ?-endotoxin from Bacillus thuringiensis var. aizawai Reviewed

    M. Himeno, H. Ihara

    MOLECULAR ACTION OF INSECTICIDES ON ION CHANNELS ACS SYMPOSIUM SERIES 雑誌   591   330 - 343   1995.01

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    Kind of work:Joint Work  

  • Specific toxicity of ?-endotoxins from Bacillus thuringiensis to Bombyx mori Reviewed

    H. Ihara, E. Kuroda, A. Wadano, M. Himeno

    Biosci. Biotech. Biochem. 雑誌   57 ( 2 )   200 - 204   1993.02

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    Kind of work:Joint Work  

  • Fluorometric assay of potential change of Bombyx mori midgut brush border membrane induced by ?-endotoxin from Bacillus thuringiensis Reviewed

    T. Uemura, H. Ihara, A. Wadano, M. Himeno

    Biosci. Biotech. Biochem. 雑誌   56 ( 12 )   1976 - 1979   1992.12

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  • Purification and some properties of isocitrate dehydrogenase of a blowfly Aldrichina grahami Reviewed

    A. Wadano, K. Miura, H. Ihara, N. Kondo, M. Taniguch

    Comp. Biochem. Physiol. 雑誌   94B ( 1 )   189 - 194   1989.01

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    Kind of work:Joint Work  

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Books and Other Publications

  • 酸化イミダゾールジペプチドによる生体機能調節

    垣花優希、笠松真吾、居原秀( Role: Joint author)

    羊土社  2020.06  ( ISBN:ISBN978-4-7581-0387-

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    Responsible for pages:1637-1643  

  • 活性硫黄研究の新展開

    居原秀、本橋ほづみ、赤池孝章( Role: Joint author)

    生化学  2019.06 

  • タンパク質・核酸の分子修飾 ニトロ化・S-グアニル化

    津々木博康、澤智裕、居原秀( Role: Joint author)

    生体の科学  2018.10 

  • 活性イオウとNOシグナル

    渡邊泰男、居原秀( Role: Joint author)

    実験医学増刊  2018.03 

  • 活性イオウ分子による細胞毒性の調節機構

    居原秀( Role: Sole author)

    日本薬理学雑誌  2016.05 

  • RSSの生体内検出とイメージング

    居原秀、大内雅也( Role: Joint author)

    細胞工学  2015.04 

  • レドックスメタボロミクス

     ( Role: Joint author)

       2013.11 

  • 細胞の酸化ストレスを量る

     ( Role: Joint author)

       2013.04 

  • 活性酸素シグナルの調節機構-ROS毒性説から脱却した新たな概念

     ( Role: Joint author)

       2013.02 

  • 硫化水素と硫化水素シグナル関連物質の検出・定量

    居原秀、井田智章( Role: Joint author)

       2012.10 

  • 喫煙と酸化ストレス Oxidative stress induced bycigarette smoking.

    岡本竜哉, 居原秀, 赤池孝章( Role: Joint author)

       2011.07 

  • 細菌性神経毒素とガングリオシド

    居原秀、小崎俊司( Role: Joint author)

       2009.06 

  • クロストリジウム神経毒素の受容体認識

    小崎俊司、居原秀( Role: Joint author)

       2009.06 

  • 生物化学実験法47「PCR実験マニュアル」原理から応用まで

    居原秀 他16名( Role: Joint author)

    学会出版センター  2002.06 

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    Responsible for pages:164-171  

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MISC

  • 【環境化学物質と生体応答】食品の超硫黄による環境化学物質の毒性制御

    笠松 真吾, 居原 秀

    Medical Science Digest   49 ( 1 )   25 - 28   2022.01( ISSN:1347-4340

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    食品中の硫黄成分の健康増強効果は古代エジプトの時代から認識されてきたが,その詳細な分子メカニズムは不明な点が多い。近年,超硫黄分子が生体内に豊富に存在し,様々な細胞機能調節に寄与していることがわかってきた。本項では,生体内および食品中に存在する超硫黄分子について概説するとともに,超硫黄分子による環境化学物質の毒性制御機構に関する最新の知見を紹介する。(著者抄録)

  • 超硫黄分子の新規分析方法の開発

    笠松 真吾,居原 秀

    生化学   93   2021.10

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  • 【生命を支える超硫黄分子の代謝と革新的な計測技術】超硫黄分子の新規分析方法の開発

    笠松 真吾, 居原 秀

    生化学   93 ( 5 )   613 - 620   2021.10( ISSN:0037-1017

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    生体内レドックスホメオスタシスは,親電子性物質の産生・除去・代謝を介して,精密に制御されている.システインパースルフィド(CysSSH)などの超硫黄分子は,種横断的に生体内で内因的に産生され,強力な抗酸化活性・レドックスシグナル制御活性を示す生理活性物質である.近年,超硫黄分子のユニークな化学特性が徐々に明らかにされるとともに,さまざまな新規分析技術が開発され,タンパク質翻訳時にCysSSHが新生鎖ペプチドに取り込まれることや,哺乳動物のミトコンドリアにおいてCysSSHとその関連硫黄代謝物がエネルギー生成およびミトコンドリア生合成に利用されていることなど,多彩な細胞機能に関与することが報告されてきている.さらに,疾患との関連や,食品中の超硫黄分子の存在様式についての知見が集まりつつある.(著者抄録)

  • 質量分析法を用いた生体内活性硫黄分子の網羅的解析

    笠松真吾 居原秀

    実験医学増刊   39 ( 13 )   2021.08

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  • いま知りたい!!硫黄生物学 みえてきた細胞内での役割 質量分析法を用いた生体内活性硫黄分子の網羅的解析

    笠松 真吾, 居原 秀

    実験医学   39 ( 13 )   2106 - 2108   2021.08( ISSN:0288-5514

  • 活性イオウ分子種によるレドックスシグナル伝達の制御(Recent Developments in Research of Reactive Sulfur Species: Regulation of redox signaling by reactive sulfur species)

    Kasamatsu Shingo, Ihara Hideshi

    Journal of Clinical Biochemistry and Nutrition   68 ( 2 )   111 - 115   2021.03( ISSN:0912-0009

  • Regulation of redox signaling by reactive sulfur species Reviewed

    Kasamatsu S, Ihara H

    J Clin Biochem Nutr   68   111 - 115   2020.11

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  • Enzymatic Regulation and Biological Functions of Reactive Cysteine Persulfides and Polysulfides Reviewed

    Tomohiro Sawa,Hozumi Motohashi , Hideshi Ihara and Takaaki Akaike

    Biomolecules   10 ( 9 )   2020.08

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  • 【食と健康を結ぶメディカルサイエンス 生体防御系を亢進し、健康の維持に働く分子機構】(第2章)食による生体防御系の活性化 酸化イミダゾールジペプチドによる生体機能調節

    垣花 優希, 笠松 真吾, 居原 秀

    実験医学   38 ( 10 )   1637 - 1643   2020.06( ISSN:0288-5514

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    イミダゾールジペプチド(imidazole-containing-dipeptides:IDPs)は、抗酸化活性などの多様な生体機能を調節する機能性食品成分として注目されている。しかし、その詳細な分子メカニズムは不明な点が多い。最近われわれは、IDPsの新規酸化修飾体である酸化IDPsの生体内生成を世界に先駆けて発見した。驚くべきことに、酸化IDPsの抗酸化活性はIDPsの数万倍高いことが明らかになった。本稿では、IDPsの基礎的な生理機能を概説するとともに、酸化IDPsの最新の知見を紹介する。(著者抄録)

  • 活性硫黄研究の新展開

    居原 秀, 本橋 ほづみ, 赤池 孝章

    生化学   91 ( 3 )   388 - 398   2019.06( ISSN:0037-1017

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    システインのチオール(SH)基に過剰な硫黄原子が付加されたシステインパースルフィドなどの「活性硫黄分子」は、生体内に多量に存在し、強い抗酸化能、レドックスシグナル制御能を有することが知られている。最近、活性硫黄分子の持つユニークな化学特性が明らかになり、それに伴い分析技術が進歩し、生体内における活性硫黄の存在様式がより詳細に明らかになってきている。セントラルドグマを成立させる上で重要な働きをするアミノアシルtRNA合成酵素の一つであるシステイニルtRNA合成酵素(CARS)が、生体内におけるシステインパースルフィドの主要な産生酵素であること、タンパク質のポリスルフィド化は、翻訳"時"に起こること、哺乳動物のミトコンドリアにおいて、システインパースルフィドとその関連代謝物が酸素分子の代わりにエネルギー産生に利用されていることなど、従来の生物学の概念を覆すような知見も得られている。さらに、疾患との関連、硫黄供給源である食品中の活性硫黄に関する知見も蓄積しつつある。(著者抄録)

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Presentations

  • イミダゾールジペプチド研究の新展開 2-オキソ-イミダゾールジペプチドは機能的な酸化生成物である

    居原 秀

    日本栄養・食糧学会大会講演要旨集  2022.05  (公社)日本栄養・食糧学会

  • 2-オキソカルノシンの抗酸化メカニズムの解明 Domestic conference

    小前奏明、笠松真吾、内田浩二、居原秀

    第94回日本生化学会大会  2021.11 

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    Presentation type:Poster presentation  

  • アルツハイマー病モデルマウス脳内における活性硫黄分子オミクス解析

    金野文香、笠松真吾、赤池孝章、居原秀

    第94回日本生化学会大会  2021.11 

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    Presentation type:Poster presentation  

  • 新規プローブによって革新された高精度超硫黄メタボロミクス Invited Domestic conference

    居原秀

    第94回日本生化学会大会  2021.11 

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    Presentation type:Poster presentation  

  • 超硫黄が切り拓く革新的オミックス研究とレドックスバイオロジー 新規プローブによって革新された高精度超硫黄メタボロミクス

    居原 秀

    日本生化学会大会プログラム・講演要旨集  2021.11  (公社)日本生化学会

  • 新規アルキル化試薬N-iodoacetyl tyrosine methyl esterを用いた超硫黄メタボローム・プロテオーム解析系の構築

    笠松 真吾, 井田 智章, 古賀 大聖, 浅田 康勝, 本橋 ほづみ, 赤池 孝章, 居原 秀

    日本生化学会大会プログラム・講演要旨集  2021.11  (公社)日本生化学会

  • アルツハイマー病モデルマウス脳内における活性硫黄分子オミクス解析

    金野 文香, 笠松 真吾, 赤池 孝章, 居原 秀

    日本生化学会大会プログラム・講演要旨集  2021.11  (公社)日本生化学会

  • 2-オキソカルノシンの抗酸化メカニズムに関する研究

    小前 奏明, 笠松 真吾, 内田 浩二, 居原 秀

    日本生化学会大会プログラム・講演要旨集  2021.11  (公社)日本生化学会

  • 2-オキソ-カルノシンはカルノシンの抗酸化活性の本体か? Domestic conference

    居原秀、笠松真吾、垣花優希、 小前奏明、松倉加奈、内田浩二

    第75回日本栄養・食糧学会大会  2021.07 

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  • 2- オキソイミダゾールジペプチドの抗酸化 活性測定 Domestic conference

    小前奏明、笠松真吾、松倉加奈、 内田浩二、居原秀

    第75回日本栄養・食糧学会大会  2021.07 

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  • 2- オキソカルノシンの活性測定 Domestic conference

    松倉加奈、笠松真吾、小前奏明、 内田浩二、居原秀

    第75回日本栄養・食糧学会大会  2021.07 

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  • 2-オキソカルノシンの活性測定

    松倉 加奈, 笠松 真吾, 小前 奏明, 内田 浩二, 居原 秀

    日本栄養・食糧学会大会講演要旨集  2021.07  (公社)日本栄養・食糧学会

  • 2-オキソイミダゾールジペプチドの抗酸化活性測定

    小前 奏明, 笠松 真吾, 松倉 加奈, 内田 浩二, 居原 秀

    日本栄養・食糧学会大会講演要旨集  2021.07  (公社)日本栄養・食糧学会

  • 2-オキソ-カルノシンはカルノシンの抗酸化活性の本体か?

    居原 秀, 笠松 真吾, 垣花 優希, 小前 奏明, 松倉 加奈, 内田 浩二

    日本栄養・食糧学会大会講演要旨集  2021.07  (公社)日本栄養・食糧学会

  • 超硫黄分子メタボロミクス Invited Domestic conference

    居原

    第1回レドックスR&D戦略委員会シンポジウム  2021.03 

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  • 2-オキソーイミダゾールジペプチドの抗酸化活性 Invited Domestic conference

    居原

    日本農芸化学会2020年度大会  2021.03 

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  • 硫黄メタボローム解析の先端技術開発 Invited Domestic conference

    居原

    第20回日本NO学会/第73回日本酸化ストレス学会合同学会  2020.10 

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  • アルツハイマー病と活性硫黄代謝系の連関の解析 Domestic conference

    居原ら

    第20回日本NO学会/第73回日本酸化ストレス学会合同学会  2020.10 

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  • 2-オキソ-カルノシンの抗炎症効果 Domestic conference

    居原ら

    第20回日本NO学会/第74回日本酸化ストレス学会合同学会  2020.10 

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  • タンパク質活性硫黄の選択的検出系の開発 Domestic conference

    居原ら

    第20回日本NO学会/第76回日本酸化ストレス学会合同学会  2020.10 

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  • 活性イオウ分子種特異的新規アルキル化試薬の合成 Domestic conference

    居原ら

    第20回日本NO学会/第76回日本酸化ストレス学会合同学会  2020.10 

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  • 新規アルキル化剤を用いた活性イオウ分子種の網羅的検出 Domestic conference

    居原ら

    第20回日本NO学会/第76回日本酸化ストレス学会合同学会  2020.10 

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  • 硫黄解析の先端技術開発 Invited Domestic conference

    居原

    第93回日本生化学会大会  2020.09 

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  • 2-オキソ-イミダゾールジペプチドは内在性の強力な抗酸化物質である Domestic conference

    居原ら

    第74回日本栄養・食糧学会大会  2020.09 

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  • 硫黄の化学的理解に立脚したカルコゲン・バイオロジー 硫黄解析の先端技術開発

    居原 秀

    日本生化学会大会プログラム・講演要旨集  2020.09  (公社)日本生化学会

  • 活性硫黄による親電子ストレス制御 Invited Domestic conference

    居原

    第47回日本毒性学会学術年会  2020.06 

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  • 硫黄代謝研究の最前線が切り拓く毒性学 活性硫黄による親電子ストレス制御

    居原 秀

    The Journal of Toxicological Sciences  2020.06  (一社)日本毒性学会

  • 酸化イミダゾールジペプチドによる細胞機能調節

    垣花 優希, 笠松 真吾, 内田 浩二, 居原 秀

    日本栄養・食糧学会大会講演要旨集  2020.04  (公社)日本栄養・食糧学会

  • 2-オキソ-イミダゾールジペプチドは内在性の強力な抗酸化物質である

    居原 秀, 垣花 優希, 内田 浩二

    日本栄養・食糧学会大会講演要旨集  2020.04  (公社)日本栄養・食糧学会

  • 細菌のタンパク質翻訳共役型システインパースルフィド合成機構の解明 Domestic conference

    居原ら

    第93回日本細菌学会総会  2020.03 

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  • 新規の抗酸化物質2-オキソ-カルノシンによる細胞機能調節 Domestic conference

    居原ら

    第19回分子予防環境医学研究会大会  2020.03 

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  • レドックスシグナルを介した神経毒性の調節機構 Domestic conference

    居原ら

    第19回分子予防環境医学研究会大会  2020.03 

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  • 1-Methyl-4-phenylpyridinium ion神経毒性における一酸化窒素/活性酸素種レドックスシグナル経路の関与 Domestic conference

    居原ら

    第19回分子予防環境医学研究会大会  2020.03 

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  • ホモアンセリンおよび酸化ホモアンセリンの測定方法の確立と生体内局在の解明 Domestic conference

    居原ら

    日本農芸化学会2020年度大会  2020.03 

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  • 培地中の硫酸塩がユーグレナの活性イオウ分子の生成動態に及ぼす影響 Domestic conference

    居原ら

    日本農芸化学会2020年度大会  2020.03 

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  • 2-オキソ-イミダゾールジペプチドの抗酸化活性 Domestic conference

    居原ら

    日本農芸化学会2020年度大会  2020.03 

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  • 食品中の活性硫黄の定量解析 Domestic conference

    居原ら

    日本農芸化学会2020年度大会  2020.03 

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  • 新規のレドックスアミノ酸;システインパースルフィド、オキソヒスチジン Invited Domestic conference

    居原

    脳内アミノ酸代謝研究会  2020.01 

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    Presentation type:Oral presentation (invited, special)  

  • 細菌のタンパク質翻訳共役型システインパースルフィド合成機構の解明

    松永 哲郎, 井田 智章, 西村 明, ジョン・ミンギョン , 守田 匡伸, 澤 智裕, 居原 秀, 本橋 ほづみ, 赤池 孝章

    日本細菌学雑誌  2020.01  日本細菌学会

  • 活性イオウ分子による親電子シグナル制御系の破綻に起因する有機水銀の神経毒性発現機構 Domestic conference

    居原ら

    メタルバイオサイエンス研究会2019  2019.10 

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  • Disruption of reactive persulfide-dependent redox signal by exposure of electrophile in neuronal cells International conference

    Ihara et al

    Persulfide Conference 2019 (1st International Conference on Persulfides and Sulfur Metabolism in Biology and Medicine)  2019.09 

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  • 活性イオウメタボロームの開発と応用展開 Domestic conference

    居原ら

    第92回日本生化学会大会  2019.09 

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  • 生体エネルギーと電子共役の複雑性制御 活性イオウメタボロームの開発と応用展開

    居原 秀

    日本生化学会大会プログラム・講演要旨集  2019.09  (公社)日本生化学会

  • 活性イオウ安定化と高精度イオウ代謝解析法の確立

    井田 智章, 松永 哲郎, 西村 明, 守田 匡伸, 居原 秀, 澤 智裕, 本橋 ほづみ, 赤池 孝章

    日本生化学会大会プログラム・講演要旨集  2019.09  (公社)日本生化学会

  • 新規の機能性物質2-オキソイミダゾールジペプチドの発見 Invited Domestic conference

    居原

    日本プロテオーム学会2019年大会 第70回日本電気泳動学会総会  2019.07 

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  • 生体成分のレドックス修飾と機能解析 新規の機能性物質2-オキソイミダゾールジペプチドの発見

    居原 秀

    電気泳動  2019.07  日本電気泳動学会

  • アルツハイマー病およびてんかんモデルマウスにおける8-nitro-cGMP産生と神経活動への影響 Domestic conference

    居原他

    第19回日本NO学会学術集会  2019.06 

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  • 腸管出血性大腸菌毒素SubABによる自然免疫抑制を介した病原性発現機構の解析 Domestic conference

    居原ら

    第19回日本NO学会学術集会  2019.06 

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  • 活性イオウ構造保護を目的とした新規アルキル化剤の作製 Domestic conference

    居原ら

    第72回 日本酸化ストレス学会学術集会  2019.06 

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  • 大豆タンパク質中の活性イオウの解析 Domestic conference

    居原ら

    第72回日本酸化ストレス学会学術集会  2019.06 

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  • 酸化イミダゾールジペプチドは新規内在性抗酸化物質である Domestic conference

    居原ら

    第72回日本酸化ストレス学会学術集会  2019.06 

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  • 全生物種横断的に発現されるシステインパースルフィド合成酵素の発見と機能解明

    井田 智章, 守田 匡伸, 松永 哲郎, 西村 明, 居原 秀, 澤 智裕, 本橋 ほづみ, 赤池 孝章

    日本細菌学雑誌  2019.03  日本細菌学会

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Industrial Property Rights

  • ポリスルフィドの安定化

    居原秀、笠松真吾

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    property_type:Patent 

  • 抗酸化剤、抗糖化剤又は抗炎症剤

    居原秀、垣花優希、内田浩二

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    property_type:Patent 

  • 特定のサルファイド化合物に結合する抗体およびその使用

    居原秀

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    property_type:Patent 

Grant-in-Aid for Scientific Research

  • 定量的超硫黄オミックス・イメージング技術の開発と標準化プロトコールの確立

    学術変革領域研究(A)  2024

Charge of on-campus class subject

  • 生物化学実験2

    2024   Weekly class   Undergraduate

  • 細胞生物化学3

    2024   Weekly class   Undergraduate

  • 細胞生物化学1

    2024   Intensive lecture   Undergraduate

  • 細胞生物化学1

    2024   Weekly class   Undergraduate

  • 生物化学特別講義B

    2024   Intensive lecture   Graduate school

  • 病態生物化学特論

    2024   Weekly class   Graduate school

  • 生物化学特別研究2A

    2024   Intensive lecture   Graduate school

  • 生物化学特別研究1A

    2024   Intensive lecture   Graduate school

  • 生物化学特別演習2A

    2024   Intensive lecture   Graduate school

  • 生物化学特別演習1A

    2024   Intensive lecture   Graduate school

  • 生物化学への招待

    2024   Weekly class   Graduate school

  • 生物化学特別講義F

    2024   Intensive lecture   Graduate school

  • 生物化学特別研究5A

    2024   Intensive lecture   Graduate school

  • 生物化学特別研究4A

    2024   Intensive lecture   Graduate school

  • 生物化学特別研究3A

    2024   Intensive lecture   Graduate school

  • 生物化学特別演習5A

    2024   Intensive lecture   Graduate school

  • 生物化学特別演習4A

    2024   Intensive lecture   Graduate school

  • 生物化学特別演習3A

    2024   Intensive lecture   Graduate school

  • 細胞生物学I

    2024   Intensive lecture   Undergraduate

  • Cell biology

    2021    

  • Biochemistry II

    2021    

  • Experiment in Biological Science II

    2021   Practical Training  

  • Instrumental Analysis in Biology

    2021    

  • Cell Biology I

    2021    

  • Molecular Biology II

    2021    

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Number of instructed thesis, researches

  • 2021

    Number of instructed the graduation thesis:

  • 2020

    Number of instructed the graduation thesis:

  • 2019

    Number of instructed the graduation thesis:

Job title

  • Job title within the department

    Graduate School of Science Department of Biological Chemistry 

    専攻長  2022.04 - 2024.03

  • Job title within the department

    School of Science Department of Biological Chemistry 

    学科長  2022.04 - 2024.03