Updated on 2024/07/12

写真a

 
FUKADA Takashi
 
Organization
Graduate School of Agriculture Department of Agricultural Biology Lecturer
School of Agriculture Department of Agricultural Biology
Title
Lecturer
Affiliation
Institute of Agriculture
Contact information
メールアドレス
Affiliation campus
Nakamozu Campus

Position

  • Graduate School of Agriculture Department of Agricultural Biology 

    Lecturer  2022.04 - Now

  • School of Agriculture Department of Agricultural Biology 

    Lecturer  2022.04 - Now

Degree

  • 博士(農学) ( Others )

Research Areas

  • Life Science / Molecular biology  / Molecular biology

  • Life Science / Cell biology  / Cell biology

  • Life Science / Molecular biology

  • Life Science / Cell biology

Research Interests

  • cytoskeleton

  • signal transduction

  • 細胞骨格

Research subject summary

  • 細胞の力学応答における細胞骨格の役割

Research Career

  • 哺乳類細胞の力学応答における細胞骨格構造と足場タンパク質の役割

    細胞骨格、力学応答、アクチン、フィラミン  Individual

Professional Memberships

  • 日本農芸化学会

    1997.04 - Now

  • 日本分子生物学会

    1993.04 - Now

  • 日本分子生物学会

  • 日本農芸化学会

Papers

  • Characterization of nucleolar localization and exclusion signals in terminal deoxynucleotidyltransferase interacting factor 2/estrogen receptor α-binding protein. Reviewed

    Takashi Fukada, Shun Shibata, Toshihiro Ueda, Katsuhiko Sasaki, Yukiko Shimoida, Kaori Senda-Murata, Kenji Sugimoto

    Bioscience, biotechnology, and biochemistry   83 ( 7 )   1255 - 1262   2019.07

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Terminal deoxynucleotidyltransferase interacting factor 2/estrogen receptor α-binding protein (TdIF2/ERBP) is a multifunctional nucleolar protein. The nucleolar localization of TdIF2/ERBP is important for its functions because it promotes ribosomal RNA transcription. However, signal sequences that direct TdIF2/ERBP to the nucleolus are not well characterized. We examined the TdIF2/ERBP sequence using truncation and mutation analyses to determine whether the nucleosome binding and C-terminal domains of TdIF2/ERBP possess nucleolar localization signals (NoLSs). In these domains, four NoLSs that could direct the mCherry protein to the nucleolus were detected. In addition, a short stretch of hydrophobic residues (VLLVL) in the center of TdIF2/ERBP acted as a nucleolar exclusion signal, which reduced the nucleolar accumulation of mCherry-NoLS fusion proteins. These results would contribute to improving the prediction of NoLSs from protein sequences. The short, transferrable localization signals would be valuable tools for understanding the association between localization and functions of nucleolar proteins. Abbreviations TdIF2: terminal deoxynucleotidyltransferase interacting factor 2; ERBP: estrogen receptor α-binding protein; EGFP: enhanced green fluorescent protein; NLS: nuclear localization signal; NoLS: nucleolar localization signal; NoES: nucleolar exclusion signal; DAPI: 4',6-diamidino-2-phenylindole.

    DOI: 10.1080/09168451.2019.1591265

    PubMed

  • Fluid shear stress applied by orbital shaking induces MG-63 osteosarcoma cells to activate ERK in two phases through distinct signaling pathways Reviewed

    Takashi Fukada, Hiroki Sakajiri, Mito Kuroda, Noriyuki Kioka, Kenji Sugimoto

    Biochem. Biophys. Rep.   9   257 - 265   2017.01

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    Authorship:Lead author, Corresponding author   Kind of work:Joint Work  

  • Live-cell imaging of HP1alpha throughout the cell cycle of mouse C3H10T1/2 cells and rhythmical flickering of heterochromatin dots in interphase Reviewed

    C. Nakagawa, A. Kawakita, T. Fukada, K. Sugimoto

    Biosci. Biotechnol. Biochem. 雑誌 Taylor & Francis   78 ( 4 )   556 - 564   2014.05

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    Kind of work:Joint Work  

  • Identification of novel nuclear protein interaction with N-terminal part of filamin A Reviewed

    H. Qiu, R. Nomiyama, K. Moriguchi, T. Fukada, K. Sugimoto

    Biosci. Biotechnol. Biochem. 雑誌 日本農芸化学会   75 ( 1 )   145 - 147   2011.01

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    Authorship:Corresponding author   Kind of work:Joint Work  

  • Development of disinfection method against drug-resistant bacteria using the antimicrobial protein HE2β1 Reviewed

    S. Ikawa, T. Fukada

    大阪府立産業技術研究所報告 雑誌 大阪府立産業技術研究所   23   1 - 5   2009

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    Kind of work:Joint Work  

  • Differential responses of mitotic spindle pole formation to microtubule-stabilizing agents epothilones A and B at low concentrations Reviewed

    Shinji Sakaushi, Kumi Nishida, Takashi Fukada, Kaori Senda-Murata, Shigenori Oka, Kenji Sugimoto

    Cell Cycle 雑誌 Landes Bioscience   7 ( 4 )   477 - 483   2008.02

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    Kind of work:Joint Work  

  • A multi-fluorescent MDA435 cell line for mitosis inhibitor studies: Simultaneous visualization of chromatin, microtubules and nuclear envelope in living cells. Reviewed

    T. Fukada, K. Senda-Murata, K. Nishida, S. Sakaushi, H. Minamikawa, M. Dotsu, S. Oka, K. Sugimoto

    Biosci. Biotechnol. Biochem. 雑誌 日本農芸化学会   71 ( 10 )   2603 - 2605   2007.10

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    Kind of work:Joint Work  

  • Dynamic behavior of FCHO1revealed by live-cell imaging microscopy: Its possible involvement in clathrin-coated vesicle formation. Reviewed

    S. Sakaushi, K. Nishida, H. Minamikawa, T. Fukada, S. Oka, K. Sugimoto

    Biosci. Biotechnol. Biochem. 雑誌 日本農芸化学会   71 ( 7 )   1764 - 1768   2007.07

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    Kind of work:Joint Work  

  • Live imaging of spindle pole disorganization in docetaxel-treated multicolor cells Reviewed

    S. Sakaushi, K. Nishida, H. Minamikawa, T. Fukada, S. Oka, K. Sugimoto

    Biochem. Biophys. Res. Commun. 雑誌 Elsevier   357 ( 3 )   655 - 660   2007.06

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    Kind of work:Joint Work  

  • Rhythmic cycle of clathrin-coated pit formation at the trans-Golgi network in human MDA-MB-435 cells Reviewed

    S. Sakaushi, K. Senda-Murata, T. Fukada, S. Oka, K. Sugimoto

    Biosci. Biotechnol. Biochem. 雑誌 日本農芸化学会   71 ( 2 )   571 - 574   2007.02

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    Kind of work:Joint Work  

  • Visualization of chromosomes and nuclear envelope in living cells for molecular dynamics studies Reviewed

    T. Fukada, K. Inoue, T. Urano, K. Sugimoto

    BioTechniques 雑誌 Eaton Publishing   37 ( 4 )   552 - 556   2004.10

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    Kind of work:Joint Work  

  • Genomic structure and 5'-flanking sequences of rat N-acetylglucosaminyltransferase I gene and regulatory role of its transcriptional diversity Reviewed

    T. Fukada, M. Ono, S. Sakata, N. Kioka, H. Sakai, T. Komano

    Biosci. Biotechnol. Biochem. 雑誌 日本農芸化学会   67 ( 7 )   1515 - 1521   2003.07

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    Kind of work:Joint Work  

  • Cytotoxic activity of Bacillus thuringiensis Cry proteins on mammalian cells transfected with cadherin-like Cry receptor gene of Bombyx mori (silkworm) Reviewed

    Y. Tsuda, F. Nakatani, K. Hashimoto, S. Ikawa, C. Matsuura, T. Fukada, K. Sugimoto, M. Himeno

    Biochem. J 雑誌 The Biochemical Society   369 ( 3 )   697 - 703   2003.02

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    Kind of work:Joint Work  

  • cDNA cloning of the Cry1Aa receptor variants from Bombyx mori and their expression in mammalian cells Reviewed

    S. Ikawa, Y. Tsuda, T. Fukada, K. Sugimoto, M. Himeno

    Biosci. Biotechnol. Biochem. 雑誌 日本農芸化学会   64 ( 12 )   2682 - 2685   2000.12

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    Kind of work:Joint Work  

  • Different response to inflammation of the multiple mRNAs of rat N-acetylglucosaminyltransferase I with variable 5'-untranslated sequences Reviewed

    T. Fukada, N. Kioka, J. Nishiu, S. Sakata, H. Sakai, M. Yamada, T. Komano

    FEBS lett. 雑誌 Federation of European Biochemical Societies   436 ( 2 )   228 - 232   1998.12

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    Kind of work:Joint Work  

  • Characterization of rat N-acetylglucosaminyltransferase I expressed in Escherichia coli Reviewed

    J. Nishiu, N. Kioka, T. Fukada, H. Sakai, T. Komano

    Biosci. Biotechnol. Biochem. 雑誌 日本農芸化学会   59 ( 9 )   1750 - 1752   1995.12

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    Kind of work:Joint Work  

  • Cloning of a cDNA encoding N-acetylglucosaminyltransferase I from rat liver and analysis of its expression in rat tissues Reviewed

    T. Fukada, K. Iida, N. Kioka, H. Sakai, T. Komano

    Biosci. Biotechnol. Biochem. 雑誌 日本農芸化学会   58 ( 1 )   200 - 201   1994.12

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    Kind of work:Joint Work  

  • Transcriptional regulation of Bacillus thuringiensis subsp. israelensis mosquito larvicidal crystal protein gene cryIVA Reviewed

    H. Yoshisue, T. Fukada, K. Yoshida, K. Sen, S. Kurosawa, H. Sakai, T. Komano

    J. Bacteriol. 雑誌 American Society for Microbiology   175 ( 9 )   2750 - 2753   1993.12

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    Kind of work:Joint Work  

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Books and Other Publications

  • 生化学実験法47 PCR実験マニュアル 原理から応用まで

    深田 尚、ほか16名( Role: Joint author)

    学会出版センター  2002.06 

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    Responsible for pages:156-160  

  • 遺伝子の構造と機能

    深田 尚、ほか28名( Role: Joint author)

    学会出版センター  1998.02 

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    Responsible for pages:173-183  

Charge of on-campus class subject

  • 応用生物科学基礎実験C

    2024   Weekly class   Undergraduate

  • 応用生物科学概論

    2024   Weekly class   Undergraduate

  • 生化学

    2024   Weekly class   Undergraduate

  • 細胞分子生物学特論

    2024   Weekly class   Graduate school

  • 応用生物科学研究実験2A

    2024   Intensive lecture   Graduate school

  • 応用生物科学研究実験1A

    2024   Intensive lecture   Graduate school

  • 応用生物科学ゼミナール2A

    2024   Intensive lecture   Graduate school

  • 応用生物科学ゼミナール1A

    2024   Intensive lecture   Graduate school

  • 応用生物科学特別研究実験3A

    2024   Intensive lecture   Graduate school

  • 応用生物科学特別研究実験2A

    2024   Intensive lecture   Graduate school

  • 応用生物科学特別研究実験1A

    2024   Intensive lecture   Graduate school

  • 生化学基礎実験

    2024   Weekly class   Undergraduate

  • 生物学実験A

    2024   Weekly class   Graduate school

  • 生物学実験A

    2024   Weekly class   Graduate school

  • 生物学実験A

    2024   Weekly class   Graduate school

  • 初年次ゼミナール

    2024   Weekly class   Graduate school

  • 生化学

    2023   Weekly class   Undergraduate

  • 生物学実験A /応用生物

    2023   Practical Training   Undergraduate

  • 生物学実験A /生命機能

    2023   Practical Training   Undergraduate

  • 生物学実験A /緑地環境

    2023   Practical Training   Undergraduate

  • 応用生物科学特別研究実験2A

    2023   Practical Training   Graduate school

  • 応用生物科学特別研究実験1A

    2023   Practical Training   Graduate school

  • 細胞分子生物学特論

    2023   Weekly class   Graduate school

  • 応用生物科学研究実験2A

    2023   Practical Training   Graduate school

  • 応用生物科学研究実験1A

    2023   Practical Training   Graduate school

  • 植物バイオサイエンス卒業研究

    2023   Intensive lecture   Undergraduate

  • 生化学基礎実験

    2023   Practical Training   Undergraduate

  • 植物バイオサイエンス演習

    2023   Intensive lecture   Undergraduate

  • 植物科学英語

    2023   Intensive lecture   Undergraduate

  • 基礎化学実験

    2023   Practical Training   Undergraduate

  • 応用生物科学特別研究実験3A

    2023   Practical Training   Graduate school

  • 応用生物科学研究プレゼンテーション

    2023   Intensive lecture   Graduate school

  • 応用生物科学特別研究実験3B

    2023   Practical Training   Graduate school

  • 応用生物科学特別研究実験2B

    2023   Practical Training   Graduate school

  • 応用生物科学特別研究実験1B

    2023   Practical Training   Graduate school

  • 応用生物科学研究実験2B

    2023   Practical Training   Graduate school

  • 応用生物科学研究実験1B

    2023   Practical Training   Graduate school

  • Research program in Plant Bioscience

    2021    

  • Special Practice in Plant Bioscience

    2021    

  • ICT Practice for Plant Bioscience

    2021    

  • Introduction to Plant Biotechnology

    2021    

  • Cellular Biochemistry

    2021    

  • Laboratory : Basic Biochemistry

    2021   Practical Training  

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