Updated on 2026/04/09

写真a

 
KOHDA Tomoko
 
Organization
Graduate School of Veterinary Science Department of Veterinary Science Lecturer
School of Veterinary Science Department of Veterinary Science
Title
Lecturer
Affiliation
Institute of Veterinary Science
Contact information
メールアドレス
Affiliation campus
Rinku Campus

Position

  • Graduate School of Veterinary Science Department of Veterinary Science 

    Lecturer  2022.04 - Now

  • School of Veterinary Science Department of Veterinary Science 

    Lecturer  2022.04 - Now

Degree

  • 博士（獣医学） （ Others ） (   Osaka Prefecture University )

Research Areas

  • Life Science / Bacteriology  / 細菌毒素

  • Life Science / Veterinary medical science  / infection disease

Research Interests

  • bacterial toxin

Research subject summary

  • 細菌毒素に対する抗体創薬

  • ボツリヌス神経毒素の作用機序の解明

Papers

  • Development of Technical Specification for <i>Clostridium botulinum</i> Detection That Can Be Used as a Reference Method in Japan

    Ovelar Maria Gabriela Quintana, Endo Miho, Yamane Shiori, Monma Chie, Kohda Tomoko, Umeda Kaoru, Okumura Kayo, Kurazono Hisao, Yamasaki Eiki

    Food Safety   14 ( 1 )   2 - 14   2026（ eISSN:21878404 ）

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    <p>The committee for the National Institute of Health Sciences Japan—The Methods for the Microbiological Examination of Foods (NIHSJ-MMEF). aims to develop domestic standard methodologies for food microbiological testing that conforms with internationally recognized standards. In this study, we developed a new qualitative detection method for <i>Clostridium botulinum</i> (NIHSJ-20TS) based on ISO/TS 17919: 2013 and validated its performance through interlaboratory study. To facilitate interlaboratory studies under stringent legal regulations, a detailed protocol for inoculum preparation was provided instead of distributing inoculated samples. In addition, we evaluated commercial DNA extraction kits as a simpler alternative to the conventional Cetyltrimethylammonium bromide (CTAB) method. The collaborative study plan was optimized as a minimal yet effective plan to verify the reproducibility and applicability of the method. After completing the validation study, the level of detection 50% (LOD<sub>50</sub>), a proposed reference value for implementation verification described in ISO 16104-3:2021, was evaluated. We believe that NIHSJ-20TS may facilitate the international acceptance of microbiological testing results generated in Japan.</p>

    DOI： 10.14252/foodsafetyfscj.d-24-00019

    PubMed

    CiNii Research

  • Gut mucin fucosylation dictates the entry of botulinum toxin complexes

    Amatsu S.

    Nature Communications   16 ( 1 )   10442   2025.12

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  • An improved immunochromatographic test strip that sensitively detects heat-labile enterotoxin produced by enterotoxigenic Escherichia coli.

    Nana Fujimoto, Emika Inoue, Nonoka Yokomizo, Sakura Hayashi, Mana Yoneyama, Tomoko Kohda, Masahiro Kusumoto, Hideyuki Arimitsu

    Journal of microbiological methods   239   107299 - 107299   2025.10（ ISSN:01677012 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Enterotoxigenic Escherichia coli (ETEC) can cause watery diarrhea not only in humans but also in domestic animals, for example, causing post-weaning diarrhea in piglets. Because the major causative factors of ETEC are heat-labile enterotoxin (LT) and heat-stable enterotoxin (ST), rapid detection methods are required for these toxins. We previously reported a prototype immunochromatographic (IC) test strip for LT comprising a mouse monoclonal antibody (mAb) and rabbit polyclonal antibody. However, the toxin detection limit of this test strip was insufficient, and the test sample bacteria needed to be cultured overnight with lincomycin supplementation to enhance LT production. Moreover, no IC test strips were available for ST. Therefore, we attempted to create a chimera protein of the B subunit of LT (LTB) and ST and develop mAbs against both LTB and ST through immunization of mice. Although nine antigen-specific mAb clones were obtained, all were LTB-specific. IC test strips prepared with the mAb 31D11 and mAb 34D4 pair were able to detect 0.15 ng/150 μL of purified LT. In addition, this IC test strip was able to detect LT in 6-h culture supernatants of clinical isolates from swine and human without requiring lincomycin supplementation. Quantification of LT levels using sandwich ELISA corroborated the IC results, indicating that the improved IC test strip enabled the detection of LT at lower concentrations and with shorter culture times than the previous method, without the need for supplements. This test strip will be useful for ETEC detection in the food hygiene and livestock hygiene fields.

    DOI： 10.1016/j.mimet.2025.107299

    PubMed

  • A novel ribosomal protection protein Tet(T2) in the tetracycline-resistant Clostridium tetani isolated from livestock bovine faeces

    Shitada C.

    Journal of Global Antimicrobial Resistance   44   432 - 434   2025.09（ ISSN:22137165 ）

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  • Gut mucin fucosylation dictates the entry of botulinum toxin complexes

    Sho Amatsu, Takuhiro Matsumura, Chiyono Morimoto, Sunanda Keisham, Yoshiyuki Goto, Tomoko Kohda, Jun Hirabayashi, Kengo Kitadokoro, Takane Katayama, Hiroshi Kiyono, Hiroaki Tateno, Masahiko Zuka, Yukako Fujinaga

    2025.08

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    Abstract

    Botulinum toxins (BoNTs) are the most potent known bacterial toxins. The BoNT complex from Clostridium botulinum B-Okra (large progenitor toxin complex (L-PTC)/B<sup>Okra</sup>, hyper-oral-toxic) exerts at least 80-fold higher oral toxicity<sup>1</sup> in mice compared with that from serotype A1 (L-PTC/A<sup>62A</sup>, non-hyper-oral-toxic). Here, we showed that L-PTC/B<sup>Okra</sup> was predominantly absorbed through enterocytes, whereas L-PTC/A<sup>62A</sup> targeted intestinal microfold cells. Furthermore, we demonstrated that α1,2-fucosylation of intestinal mucin determined the oral toxicity of L-PTCs as well as their entry routes; more specifically, these routes were governed by the carbohydrate-binding spectrum of hemagglutinin (HA) complex, which is one of the L-PTC components. Disruption of fucosylation in fucosyltrasferase-2 (Fut2)-null mice hindered the intestinal mucin penetration of L-PTC/B<sup>Okra</sup> via HA and reduced the susceptibility to its oral intoxication. Our data establish the molecular mechanism by which the oral toxicity of BoNTs is increased after they cross intestinal mucus layers.

    DOI： 10.1101/2025.08.30.672933

    PubMed

  • Characterization of α-hemolysin-specific monoclonal antibodies for detection in the culture supernatant of pathogenic Escherichia coli strains(タイトル和訳中)

    Fujimoto Haruki, Kida Sayaka, Kohda Tomoko, Mukamoto Masafumi, Kusumoto Masahiro, Arimitsu Hideyuki

    The Journal of Veterinary Medical Science   87 ( 8 )   919 - 923   2025.08（ ISSN:0916-7250 ）

  • Characterization of α-hemolysin-specific monoclonal antibodies for detection in the culture supernatant of pathogenic <i>Escherichia coli</i> strains

    FUJIMOTO Haruki, KIDA Sayaka, KOHDA Tomoko, MUKAMOTO Masafumi, KUSUMOTO Masahiro, ARIMITSU Hideyuki

    Journal of Veterinary Medical Science   87 ( 8 )   919 - 923   2025（ ISSN:09167250 ） （ eISSN:13477439 ）

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    Publishing type：Research paper (scientific journal)  

    <p>α-Hemolysin (α-Hly) is produced by pathogenic <i>Escherichia coli</i> and is implicated in a variety of diseases. In the swine, enterotoxigenic <i>E. coli</i> (ETEC) and Shiga toxin-producing <i>E. coli</i> (STEC) strains cause post-weaning diarrhea and edema disease, respectively, and most of these strains are hemolytic. However, the role of α-Hly in ETEC and STEC infection is unclear. Here, we prepared α-Hly-specific monoclonal antibodies by immunizing animals with one-third of the carboxy end of recombinant α-Hly. The four α-Hly-specific antibodies obtained reacted with α-Hly in the culture supernatant of clinical isolates in enzyme-linked immunosorbent assay and western blot analysis. In addition, because these four monoclonal antibodies inhibited <i>E. coli</i>-induced hemolysis, these antibodies might be useful tools for investigating the significance of α-Hly in pathogenic strains.</p>

    DOI： 10.1292/jvms.25-0072

    PubMed

    CiNii Research

  • Nsp14 of SARS-CoV-2 inhibits mRNA processing and nuclear export by targeting the nuclear cap-binding complex

    Katahira J. Ohmae T. Yasugi M. Sasaki R. Itoh Y. Kohda T. Hieda M. Hirai Y M. Okamoto T. Miyamoto Y.

    Nucleic Acids Research   51 ( 14 )   7602 - 7618   2023.08（ ISSN:03051048 ）

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  • A Pilot Study of A2NTX, a Novel Low-Molecular-Weight Neurotoxin Derived from Subtype A2 for Post-Stroke Lower Limb Spasticity: Comparison with OnabotulinumtoxinA

    Kaji R. Miyashiro A. Sato N. Fukumoto T. Takeuchi T. Miyamoto R. Kohda T. Izumi Y. Kozaki S.

    Toxins   14 ( 11 )   2022.10

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    International / domestic magazine：International journal  

    DOI： 10.3390/toxins14110739

    PubMed

  • 水様下痢を呈したカピバラ(Hydrochoerus hydrochaeris)2症例における共生繊毛虫の消失

    松尾 加代子, 竹内 由佳, 常盤 俊大, 所 正治, 酒井 洋樹, 幸田 知子

    日本野生動物医学会誌   27 ( 2 )   139 - 144   2022.09（ ISSN:1342-6133 ）

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    International / domestic magazine：Domestic journal  

    動物展示施設で飼育されたカピバラ6頭のうち2頭に下痢がみられ、メトロニダゾールの投与により一時的に便性状の改善を認めたが、その後に再度水様下痢を呈し死亡した。生存個体の糞便には腸管に寄生するオフリオスコレックス科繊毛虫を多数認めたが、これらの繊毛虫はメトロニダゾール投与歴のある1頭および死亡2症例では検出できなかった。これらの結果より、植物食であるカピバラの消化吸収の大部分を担う盲腸内発酵を支える共生繊毛虫への影響も今後考慮する必要があると思われた。(著者抄録)

  • 豚浮腫病の臨床検体のエンリッチメント培養における志賀毒素2eの免疫クロマトグラフィー試験の評価(Evaluation of immunochromatographic test of Shiga toxin 2e in enrichment cultures of swine edema disease clinical samples)

    Arimitsu Hideyuki, Kohda Tomoko, Mukamoto Masafumi, Kusumoto Masahiro

    The Journal of Veterinary Medical Science   83 ( 12 )   1913 - 1917   2021.12（ ISSN:0916-7250 ）

  • Clinical Safety and Tolerability of A2NTX, a Novel Low-Molecular-Weight Neurotoxin Derived from Botulinum Neurotoxin Subtype A2, in Comparison with Subtype A1 Toxins Reviewed

    Takeuchi T. Okuno T. Miyashiro A. Kohda T. Miyamoto R. Izumi Y. Kozaki S. Kaji R.

    Toxins   13 ( 824 )   2021.11

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    DOI： 10.3390/ toxins13110824

  • The cytotoxicity and molecular mechanisms of the Clostridium perfringens NetB toxin

    83 ( 2 )   187 - 194   2021.02（ ISSN:0916-7250 ）

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    International / domestic magazine：International journal  

  • Analysis of a plasmid encoding botulinum neurotoxin type G gene in Clostridium argentinense Reviewed

    Sakaguchi Y, Uchiyama J, Take A, Gotoh K, Sakaguchi M, Suzuki T, Yamamoto Y, Hosomi K, Kohda T, Mukamoto M, Kozaki S, Hayashi S, Oguma K.

    Anaerobe 雑誌   66   2020.12

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    Kind of work：Joint Work  

  • Analysis of a plasmid encoding botulinum neurotoxin type G gene in Clostridium argentinense.

    Yoshihiko Sakaguchi, Jumpei Uchiyama, Akira Také, Kazuyoshi Gotoh, Masakiyo Sakaguchi, Tomonori Suzuki, Yumiko Yamamoto, Koji Hosomi, Tomoko Kohda, Masafumi Mukamoto, Shunji Kozaki, Shunji Hayashi, Keiji Oguma

    Anaerobe   66   102281 - 102281   2020.12

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Clostridium argentinense produces botulinum neurotoxin type G (BoNT/G). We sequenced and analyzed the plasmid harboring the bont/G gene, designated pCAG, in C. argentinense strain 2740. The pCAG consisted of 140,070 bp containing the bont/G gene cluster. Although this gene cluster showed high similarities in its DNA sequence and ORF arrangement to those of other bont gene clusters, the other regions of the plasmid did not. A phylogenetic study suggested that pCAG had a unique evolutionary history compared with other clostridial bont-harboring plasmids. This suggests that pCAG is possibly a novel type of plasmid expressing the bont/G gene in C. argentinense.

    DOI： 10.1016/j.anaerobe.2020.102281

    PubMed

  • ボツリヌス神経毒素A亜型2の細胞内移行領域は神経細胞への侵入を強く誘導する(Translocation domain of botulinum neurotoxin A subtype 2 potently induces entry into neuronal cells)

    Kohda Tomoko, Tsukamoto Kentaro, Torii Yasushi, Kozaki Shunji, Mukamoto Masafumi

    Microbiology and Immunology   64 ( 7 )   502 - 511   2020.07（ ISSN:0385-5600 ）

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    神経細胞へのボツリヌス神経毒素(BoNT)侵入と関連のあるN末端(HN)領域について調べるため、BoNT血清型A(BoNT/A1)とBoNT/A2のHNをBoNT/A2とBoNT/A1のHNに置換したキメラBoNP/A121とBoNT/A212を作成した。野生型とキメラのBoNT/Aの毒性は類似していた。しかし、神経細胞のSNAP-25開裂活性は異なっていた。BoNP/A121とBoNT/A2は同じような高いSNAP-25開裂活性を示した。以上より、BoNT/A1よりもBoNT/A2の活性が高いのは、BoNT/A2HNによると考えられた。

  • Fully Human Monoclonal Antibodies Effectively Neutralizing Botulinum Neurotoxin Serotype B.

    Takuhiro Matsumura, Sho Amatsu, Ryo Misaki, Masahiro Yutani, Anariwa Du, Tomoko Kohda, Kazuhito Fujiyama, Kazuyoshi Ikuta, Yukako Fujinaga

    Toxins   12 ( 5 )   2020.05

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Botulinum neurotoxin (BoNT) is the most potent natural toxin known. Of the seven BoNT serotypes (A to G), types A, B, E, and F cause human botulism. Treatment of human botulism requires the development of effective toxin-neutralizing antibodies without side effects such as serum sickness and anaphylaxis. In this study, we generated fully human monoclonal antibodies (HuMAbs) against serotype B BoNT (BoNT/B1) using a murine-human chimera fusion partner cell line named SPYMEG. Of these HuMAbs, M2, which specifically binds to the light chain of BoNT/B1, showed neutralization activity in a mouse bioassay (approximately 10 i.p. LD50/100 µg of antibody), and M4, which binds to the C-terminal of heavy chain, showed partial protection. The combination of two HuMAbs, M2 (1.25 µg) and M4 (1.25 µg), was able to completely neutralize BoNT/B1 (80 i.p. LD50) with a potency greater than 80 i.p. LD50/2.5 µg of antibodies, and was effective both prophylactically and therapeutically in the mouse model of botulism. Moreover, this combination showed broad neutralization activity against three type B subtypes, namely BoNT/B1, BoNT/B2, and BoNT/B6. These data demonstrate that the combination of M2 and M4 is promising in terms of a foundation for new human therapeutics for BoNT/B intoxication.

    DOI： 10.3390/toxins12050302

    PubMed

  • Response to “Standardized methods must be used tocompare the properties of botulinum toxin serotypes. Reviewed

    Kohda T, Nakamura K, Hosomi K, Torii Y, Kozaki S, Mukamoto M.

    Microbiol Immunol. 雑誌   61   2017.11

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    Kind of work：Joint Work  

  • Botulinum neurotoxin A2 reduces incidence of seizures in mouse models of temporal lobe epilepsy. Reviewed

    Kato K, Akaike N, Kohda T, Torii Y, Goto Y, Harakawa T, Ginnaga A, Kaji R, Kozaki S.

    Toxicon 雑誌   74   109 - 115   2013.11

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    Kind of work：Joint Work  

  • Botulinum neurotoxin A2 reduces incidence of seizures in mouse models of temporal lobe epilepsy. Reviewed

    Kato K, Akaike N, Kohda T, Torii Y, Goto Y, Harakawa T, Ginnaga A, Kaji R, Kozaki S.

    Toxicon 雑誌   74   109 - 115   2013.11

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    Kind of work：Joint Work  

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Books and Other Publications

  • 動物衛生学　第2版

    幸田　知子（ Role： Contributor ,  牛ボツリヌス症）

    文栄堂出版  2024.03 

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    Total pages：467   Responsible for pages：445-445   Book type：Textbook, survey, introduction

MISC

  • 我が国における牛ボツリヌス症の現状と課題

    幸田 知子, 中村 佳司

    家畜感染症学会誌   14 ( 1 )   11 - 19   2025.04（ ISSN:2186-7208 ）

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    ボツリヌス症は、ボツリヌス菌が産生するボツリヌス神経毒素によって起こる弛緩性麻痺疾患である。ボツリヌス菌は偏性嫌気性グラム陽性の有芽細胞菌であり、生化学的性状によりI-IV群に分類されるほか、産生する神経毒素の抗原性によってA～Gの7型に分けられる。家畜・家禽のボツリヌス症はIII群ボツリヌス菌に属するCおよびD型菌によって起こり、牛のボツリヌス症の原因は主にD型菌であることが多い。牛ボツリヌス症の発生は国内外で報告され、特に国内においては、散発的な発生が毎年報告されており、畜産業界に少なからぬ被害を与えている一方、国内における正確な発生件数、発生頭数は不明でありその現況は広く知られていない。本稿では牛におけるボツリヌス症の概要について、特に感染経路を含めた発症機序、主要病原因子であるD/Cモザイク毒素の特性、検査方法を中心に報告する。さらに、現在NCBIのデータベースで公開されている海外株のゲノム配列に、我々が取得した未公開の国内株のゲノム配列を加えた、合計73株の牛ボツリヌス症由来菌の全ゲノム系統遺伝学的解析結果を示し、本症の原因菌の遺伝的な特性について考察する。今後の本症への対策において、汎用的かつ効率的な検査法の確立は特に重要な課題のひとつであり、従来の毒素検出法に加えて、毒素産生菌の性状に着目した新たな選択的分離法の開発が必要であると考えられる。(著者抄録)

  • 牛ボツリヌス症

    幸田　知子

    一般社団法人　全国肉用牛振興基金協会   2023.11

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    Authorship：Lead author   Publishing type：Article, review, commentary, editorial, etc. (other)  

Presentations

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Grant-in-Aid for Scientific Research

  • ボツリヌス神経毒素のエンドソーム膜通過機構の解析

    Grant-in-Aid for Scientific Research(C)  2028

  • ボツリヌス神経毒素のエンドソーム膜通過機構の解析

    Grant-in-Aid for Scientific Research(C)  2027

  • ボツリヌス神経毒素のエンドソーム膜通過機構の解析

    Grant-in-Aid for Scientific Research(C)  2026.04

  • ボツリヌス神経毒素のエンドソーム膜通過機構の解析

    Grant-in-Aid for Scientific Research(C)  2026

  • ヒト化ボツリヌス毒素抗体の抗体医薬品としての応用

    Grant-in-Aid for Scientific Research(C)  2024

  • ヒト化ボツリヌス毒素抗体の抗体医薬品としての応用

    Grant-in-Aid for Scientific Research(C)  2023

  • ヒト化ボツリヌス毒素抗体の抗体医薬品としての応用

    Grant-in-Aid for Scientific Research(C)  2022

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Acceptance of Researcher

  • 2021  Number of researchers：1

Charge of on-campus class subject

  • 獣医体験演習

    2025   Weekly class   Undergraduate

  • 畜産学実習

    2025   Intensive lecture   Undergraduate

  • 畜産学実習

    2025   Intensive lecture   Undergraduate

  • 獣医衛生学実習

    2025   Weekly class   Undergraduate

  • 臨床基礎実習

    2025   Weekly class   Undergraduate

  • 公務員獣医師インターンシップ

    2025   Intensive lecture   Undergraduate

  • 獣医伝染病学A

    2025   Weekly class   Undergraduate

  • 獣医感染制御学実習

    2025   Intensive lecture   Undergraduate

  • 産業動物病性鑑定実習

    2025   Intensive lecture   Undergraduate

  • 臨床基礎実習

    2025   Weekly class   Undergraduate

  • 食品衛生学基礎実習

    2025   Intensive lecture   Undergraduate

  • 獣医衛生学実習

    2025   Weekly class   Undergraduate

  • 獣医科学英語演習

    2025   Intensive lecture   Undergraduate

  • 感染症制御学特論

    2025   Intensive lecture   Graduate school

  • 獣医衛生学

    2025   Weekly class   Undergraduate

  • 獣医伝染病学A

    2025   Weekly class   Undergraduate

  • 獣医伝染病学B

    2025   Weekly class   Undergraduate

  • 獣医伝染病学B

    2025   Intensive lecture   Undergraduate

  • 臨床基礎実習

    2025   Weekly class   Undergraduate

  • 臨床基礎実習

    2024   Weekly class   Undergraduate

  • 獣医伝染病学A

    2024   Weekly class   Undergraduate

  • 獣医感染制御学実習

    2024   Intensive lecture   Undergraduate

  • 産業動物病性鑑定実習

    2024   Intensive lecture   Undergraduate

  • 獣医体験演習

    2024   Weekly class   Undergraduate

  • 畜産学実習

    2024   Intensive lecture   Undergraduate

  • 畜産学実習

    2024   Intensive lecture   Undergraduate

  • 獣医衛生学実習

    2024   Weekly class   Undergraduate

  • 臨床基礎実習

    2024   Weekly class   Undergraduate

  • 感染症制御学特論

    2024   Intensive lecture   Graduate school

  • 生命環境科学概論

    2024   Intensive lecture   Undergraduate

  • 獣医衛生学実習

    2024   Weekly class   Undergraduate

  • 獣医科学英語演習

    2024   Intensive lecture   Undergraduate

  • 小動物基礎臨床実習

    2024   Intensive lecture   Undergraduate

  • 獣医衛生学

    2024   Weekly class   Undergraduate

  • 獣医伝染病学A

    2024   Weekly class   Undergraduate

  • 獣医伝染病学B

    2024   Weekly class   Undergraduate

  • 産業動物病性鑑定実習

    2024   Intensive lecture   Undergraduate

  • 獣医感染制御学実習

    2024   Intensive lecture   Undergraduate

  • 獣医感染制御学実習

    2023   Intensive lecture   Undergraduate

  • 獣医伝染病学A

    2023   Weekly class   Undergraduate

  • 産業動物病性鑑定実習

    2023   Intensive lecture   Undergraduate

  • 獣医体験演習

    2023   Weekly class   Undergraduate

  • 畜産学実習

    2023   Intensive lecture   Undergraduate

  • Practice in Food Hygiene

    2021   Practical Training  

  • Practice in Pathobiological Diagnoses for Live Stock Diseases

    2021   Practical Training  

  • Practice in Veterinary Infectious Disease Control

    2021   Practical Training  

  • Practice in Veterinary Hygiene

    2021   Practical Training  

  • Practice in Veterinary Hygiene

    2021   Practical Training  

  • Infectious Diseases of Animals A

    2021    

  • First Year Seminar

    2021    

  • Biology B

    2021    

  • Basic clinical practices

    2021   Practical Training  

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Number of instructed thesis, researches

  • 2025

    Number of instructed the graduation thesis：2  Number of graduation thesis reviews：2

  • 2024

    Number of instructed the graduation thesis：2  Number of graduation thesis reviews：2

  • 2023

    Number of instructed the graduation thesis：1  Number of graduation thesis reviews：2

  • 2022

    Number of instructed the graduation thesis：1 

  • 2021

    Number of instructed the graduation thesis：1