Updated on 2024/07/13

写真a

 
KOHDA Tomoko
 
Organization
Graduate School of Veterinary Science Department of Veterinary Science Lecturer
School of Veterinary Science Department of Veterinary Science
Title
Lecturer
Affiliation
Institute of Veterinary Science
Contact information
メールアドレス
Affiliation campus
Rinku Campus

Position

  • Graduate School of Veterinary Science Department of Veterinary Science 

    Lecturer  2022.04 - Now

  • School of Veterinary Science Department of Veterinary Science 

    Lecturer  2022.04 - Now

Degree

  • 博士（獣医学） （ Others ） (   Osaka Prefecture University )

Research Areas

  • Life Science / Bacteriology  / 細菌毒素

  • Life Science / Veterinary medical science  / infection disease

Research Interests

  • bacterial toxin

Papers

  • Nsp14 of SARS-CoV-2 inhibits mRNA processing and nuclear export by targeting the nuclear cap-binding complex

    Katahira J. Ohmae T. Yasugi M. Sasaki R. Itoh Y. Kohda T. Hieda M. Hirai Y M. Okamoto T. Miyamoto Y.

    Nucleic Acids Research   51 ( 14 )   7602 - 7618   2023.08（ ISSN:03051048 ）

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  • A Pilot Study of A2NTX, a Novel Low-Molecular-Weight Neurotoxin Derived from Subtype A2 for Post-Stroke Lower Limb Spasticity: Comparison with OnabotulinumtoxinA

    Kaji R. Miyashiro A. Sato N. Fukumoto T. Takeuchi T. Miyamoto R. Kohda T. Izumi Y. Kozaki S.

    Toxins   14 ( 11 )   2022.10

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    International / domestic magazine：International journal  

    DOI： 10.3390/toxins14110739

    PubMed

  • 水様下痢を呈したカピバラ(Hydrochoerus hydrochaeris)2症例における共生繊毛虫の消失

    松尾 加代子, 竹内 由佳, 常盤 俊大, 所 正治, 酒井 洋樹, 幸田 知子

    日本野生動物医学会誌   27 ( 2 )   139 - 144   2022.09（ ISSN:1342-6133 ）

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    International / domestic magazine：Domestic journal  

    動物展示施設で飼育されたカピバラ6頭のうち2頭に下痢がみられ、メトロニダゾールの投与により一時的に便性状の改善を認めたが、その後に再度水様下痢を呈し死亡した。生存個体の糞便には腸管に寄生するオフリオスコレックス科繊毛虫を多数認めたが、これらの繊毛虫はメトロニダゾール投与歴のある1頭および死亡2症例では検出できなかった。これらの結果より、植物食であるカピバラの消化吸収の大部分を担う盲腸内発酵を支える共生繊毛虫への影響も今後考慮する必要があると思われた。(著者抄録)

  • 豚浮腫病の臨床検体のエンリッチメント培養における志賀毒素2eの免疫クロマトグラフィー試験の評価(Evaluation of immunochromatographic test of Shiga toxin 2e in enrichment cultures of swine edema disease clinical samples)

    Arimitsu Hideyuki, Kohda Tomoko, Mukamoto Masafumi, Kusumoto Masahiro

    The Journal of Veterinary Medical Science   83 ( 12 )   1913 - 1917   2021.12（ ISSN:0916-7250 ）

  • Clinical Safety and Tolerability of A2NTX, a Novel Low-Molecular-Weight Neurotoxin Derived from Botulinum Neurotoxin Subtype A2, in Comparison with Subtype A1 Toxins Reviewed

    Takeuchi T. Okuno T. Miyashiro A. Kohda T. Miyamoto R. Izumi Y. Kozaki S. Kaji R.

    Toxins   13 ( 824 )   2021.11

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    DOI： 10.3390/ toxins13110824

  • The cytotoxicity and molecular mechanisms of the Clostridium perfringens NetB toxin

    83 ( 2 )   187 - 194   2021.02（ ISSN:0916-7250 ）

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    International / domestic magazine：International journal  

  • Analysis of a plasmid encoding botulinum neurotoxin type G gene in Clostridium argentinense Reviewed

    Sakaguchi Y, Uchiyama J, Take A, Gotoh K, Sakaguchi M, Suzuki T, Yamamoto Y, Hosomi K, Kohda T, Mukamoto M, Kozaki S, Hayashi S, Oguma K.

    Anaerobe 雑誌   66   2020.12

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    Kind of work：Joint Work  

  • Analysis of a plasmid encoding botulinum neurotoxin type G gene in Clostridium argentinense.

    Yoshihiko Sakaguchi, Jumpei Uchiyama, Akira Také, Kazuyoshi Gotoh, Masakiyo Sakaguchi, Tomonori Suzuki, Yumiko Yamamoto, Koji Hosomi, Tomoko Kohda, Masafumi Mukamoto, Shunji Kozaki, Shunji Hayashi, Keiji Oguma

    Anaerobe   66   102281 - 102281   2020.12

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Clostridium argentinense produces botulinum neurotoxin type G (BoNT/G). We sequenced and analyzed the plasmid harboring the bont/G gene, designated pCAG, in C. argentinense strain 2740. The pCAG consisted of 140,070 bp containing the bont/G gene cluster. Although this gene cluster showed high similarities in its DNA sequence and ORF arrangement to those of other bont gene clusters, the other regions of the plasmid did not. A phylogenetic study suggested that pCAG had a unique evolutionary history compared with other clostridial bont-harboring plasmids. This suggests that pCAG is possibly a novel type of plasmid expressing the bont/G gene in C. argentinense.

    DOI： 10.1016/j.anaerobe.2020.102281

    PubMed

  • ボツリヌス神経毒素A亜型2の細胞内移行領域は神経細胞への侵入を強く誘導する(Translocation domain of botulinum neurotoxin A subtype 2 potently induces entry into neuronal cells)

    Kohda Tomoko, Tsukamoto Kentaro, Torii Yasushi, Kozaki Shunji, Mukamoto Masafumi

    Microbiology and Immunology   64 ( 7 )   502 - 511   2020.07（ ISSN:0385-5600 ）

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    神経細胞へのボツリヌス神経毒素(BoNT)侵入と関連のあるN末端(HN)領域について調べるため、BoNT血清型A(BoNT/A1)とBoNT/A2のHNをBoNT/A2とBoNT/A1のHNに置換したキメラBoNP/A121とBoNT/A212を作成した。野生型とキメラのBoNT/Aの毒性は類似していた。しかし、神経細胞のSNAP-25開裂活性は異なっていた。BoNP/A121とBoNT/A2は同じような高いSNAP-25開裂活性を示した。以上より、BoNT/A1よりもBoNT/A2の活性が高いのは、BoNT/A2HNによると考えられた。

  • Fully Human Monoclonal Antibodies Effectively Neutralizing Botulinum Neurotoxin Serotype B.

    Takuhiro Matsumura, Sho Amatsu, Ryo Misaki, Masahiro Yutani, Anariwa Du, Tomoko Kohda, Kazuhito Fujiyama, Kazuyoshi Ikuta, Yukako Fujinaga

    Toxins   12 ( 5 )   2020.05

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Botulinum neurotoxin (BoNT) is the most potent natural toxin known. Of the seven BoNT serotypes (A to G), types A, B, E, and F cause human botulism. Treatment of human botulism requires the development of effective toxin-neutralizing antibodies without side effects such as serum sickness and anaphylaxis. In this study, we generated fully human monoclonal antibodies (HuMAbs) against serotype B BoNT (BoNT/B1) using a murine-human chimera fusion partner cell line named SPYMEG. Of these HuMAbs, M2, which specifically binds to the light chain of BoNT/B1, showed neutralization activity in a mouse bioassay (approximately 10 i.p. LD50/100 µg of antibody), and M4, which binds to the C-terminal of heavy chain, showed partial protection. The combination of two HuMAbs, M2 (1.25 µg) and M4 (1.25 µg), was able to completely neutralize BoNT/B1 (80 i.p. LD50) with a potency greater than 80 i.p. LD50/2.5 µg of antibodies, and was effective both prophylactically and therapeutically in the mouse model of botulism. Moreover, this combination showed broad neutralization activity against three type B subtypes, namely BoNT/B1, BoNT/B2, and BoNT/B6. These data demonstrate that the combination of M2 and M4 is promising in terms of a foundation for new human therapeutics for BoNT/B intoxication.

    DOI： 10.3390/toxins12050302

    PubMed

  • Response to “Standardized methods must be used tocompare the properties of botulinum toxin serotypes. Reviewed

    Kohda T, Nakamura K, Hosomi K, Torii Y, Kozaki S, Mukamoto M.

    Microbiol Immunol. 雑誌   61   2017.11

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    Kind of work：Joint Work  

  • Botulinum neurotoxin A2 reduces incidence of seizures in mouse models of temporal lobe epilepsy. Reviewed

    Kato K, Akaike N, Kohda T, Torii Y, Goto Y, Harakawa T, Ginnaga A, Kaji R, Kozaki S.

    Toxicon 雑誌   74   109 - 115   2013.11

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    Kind of work：Joint Work  

  • Botulinum neurotoxin A2 reduces incidence of seizures in mouse models of temporal lobe epilepsy. Reviewed

    Kato K, Akaike N, Kohda T, Torii Y, Goto Y, Harakawa T, Ginnaga A, Kaji R, Kozaki S.

    Toxicon 雑誌   74   109 - 115   2013.11

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    Kind of work：Joint Work  

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Books and Other Publications

  • 動物衛生学　第2版

    幸田　知子（ Role： Contributor ,  牛ボツリヌス症）

    文栄堂出版  2024.03 

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    Total pages：467   Responsible for pages：445-445   Book type：Textbook, survey, introduction

MISC

  • 牛ボツリヌス症

    幸田　知子

    一般社団法人　全国肉用牛振興基金協会   2023.11

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    Authorship：Lead author   Publishing type：Article, review, commentary, editorial, etc. (other)  

Presentations

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Grant-in-Aid for Scientific Research

  • ヒト化ボツリヌス毒素抗体の抗体医薬品としての応用

    Grant-in-Aid for Scientific Research(C)  2024

  • ヒト化ボツリヌス毒素抗体の抗体医薬品としての応用

    Grant-in-Aid for Scientific Research(C)  2023

  • ヒト化ボツリヌス毒素抗体の抗体医薬品としての応用

    Grant-in-Aid for Scientific Research(C)  2022

Acceptance of Researcher

  • 2021  Number of researchers：1

Charge of on-campus class subject

  • 獣医感染制御学実習

    2024   Intensive lecture   Undergraduate

  • 産業動物病性鑑定実習

    2024   Intensive lecture   Undergraduate

  • 獣医衛生学

    2024   Weekly class   Undergraduate

  • 小動物基礎臨床実習

    2024   Intensive lecture   Undergraduate

  • 獣医伝染病学B

    2024   Weekly class   Undergraduate

  • 獣医伝染病学A

    2024   Weekly class   Undergraduate

  • 感染症制御学特論

    2024   Intensive lecture   Graduate school

  • 獣医科学英語演習

    2024   Intensive lecture   Undergraduate

  • 獣医衛生学実習

    2024   Weekly class   Undergraduate

  • 生命環境科学概論

    2024   Intensive lecture   Undergraduate

  • 畜産学実習

    2023   Intensive lecture   Undergraduate

  • 獣医体験演習

    2023   Weekly class   Undergraduate

  • 産業動物病性鑑定実習

    2023   Intensive lecture   Undergraduate

  • 獣医伝染病学A

    2023   Weekly class   Undergraduate

  • 獣医感染制御学実習

    2023   Intensive lecture   Undergraduate

  • Practice in Food Hygiene

    2021   Practical Training  

  • Practice in Pathobiological Diagnoses for Live Stock Diseases

    2021   Practical Training  

  • Practice in Veterinary Infectious Disease Control

    2021   Practical Training  

  • Practice in Veterinary Hygiene

    2021   Practical Training  

  • Practice in Veterinary Hygiene

    2021   Practical Training  

  • Infectious Diseases of Animals A

    2021    

  • First Year Seminar

    2021    

  • Biology B

    2021    

  • Basic clinical practices

    2021   Practical Training  

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Number of instructed thesis, researches

  • 2024

    Number of instructed the graduation thesis：2  Number of graduation thesis reviews：2

  • 2023

    Number of instructed the graduation thesis：1  Number of graduation thesis reviews：2

  • 2022

    Number of instructed the graduation thesis：1 

  • 2021

    Number of instructed the graduation thesis：1