Environmental Science/Agriculture Science / Environmental agriculture

日本生化学会

1998.04 - Now

日本農芸化学会

日本生化学会

日本ビタミン学会

Wax Ester Production Coupled with Anaerobic Respiratory Chain in <i>Euglena gracilis</i> Invited

NAKAZAWA Masami

Oleoscience   23 ( 2 )   73 - 78   2023.02（ ISSN:13458949 ）

A single mutation Asp43Arg was increased 2.5-fold the catalytic activity and maintained the stability of cold-adapted endo-1,4-beta glucanase (Ef-EG2) from Eisenia fetida

Kuroki C.

Current Research in Biotechnology   5   2023.01

Euglena International Network (EIN): Driving euglenoid biotechnology for the benefit of a challenged world

Ebenezer T.G.E.

Biology open   11 ( 11 )   2022.11

Novel acid trehalase belonging to glycoside hydrolase family 37 from <i>Pleurotus</i> sp.: cloning, expression and characterization

Tsutsumi Gaku, Kuroki Chikako, Kamei Kengo, Kusuda Mizuho, Nakazawa Masami, Sakamoto Tatsuji, Ishikawa Mariko, Harada Shinji, Kobayashi Hitoshi, Ouchi Kenji, Inatomi Satoshi, Sakaguchi Minoru, Iwamoto Takeo, Ueda Mitsuhiro

Mycoscience   63 ( 6 )   284 - 292   2022.10（ ISSN:13403540 ）

Gelation of konjac glucomannan by acetylmannan esterases from Aspergillus oryzae

Saito M.

Enzyme and Microbial Technology   160   110075   2022.10（ ISSN:01410229 ）

NADPH-to-NADH conversion by mitochondrial transhydrogenase is indispensable for sustaining anaerobic metabolism in Euglena gracilis Reviewed

Masami Nakazawa, Mutsuki Takahashi, Ryuta Hayashi, Yuki Matsubara, Yuichiro Kashiyama, Mitsuhiro Ueda, Hiroshi Inui, Tatsuji Sakamoto

FEBS Letters FEBS press   595 ( 23 )   2922 - 2930   2021.12

Homogalacturonan and xylogalacturonan region specificity of self-cloning vector-expressed pectin methylesterases (AoPME1–3) in Aspergillus oryzae. Reviewed

Yamada, H., Kubo, S., Kunishige, Y., Azuma, H., Kotani, Y., Handa, S., Nakazawa, M., Ueda, M., Hasegawa, Y., Sakamoto, T.

Enzyme Microb. Technol. Elsevier   150   2021.10

Novel cold-adapted raw-starch digesting α-amylases from Eisenia fetida: gene cloning, expression, and characterization. Reviewed

Tsukamoto, K., Ariki, S., Nakazawa, M., Sakamoto, T., Ueda, M.

Biotechnol. Rep. Elsevier   31   2021.09

コナサナギタケ Paecilomyces farinosus 由来のトリプシン様セリンエンドペプチダーゼの精製と性質 Reviewed

上田光宏，森本和樹，楠田瑞穂，亀井健吾，中澤昌美，阪本龍司，坂口　実，小林　仁，大内謙二，稲冨　聡

日本きのこ学会誌 日本きのこ学会   29 ( 1 )   30 - 33   2021.01

Determination of chemical structure of pea pectin by using pectinolytic enzymes. Reviewed

Noguchi M, Hasegawa Y, Suzuki S, Nakazawa M, Ueda M, Sakamoto T.

Carbohydr Polym 雑誌   231   2020.03

Characterization of three GH35 β-galactosidases, enzymes able to shave galactosyl residues linked to rhamnogalacturonan in pectin, from Penicillium chrysogenum 31B Reviewed

Kondo T, Nishimura Y, Matsuyama K, Ishimaru M, Nakazawa M, Ueda M, Sakamoto T.

Appl Microbiol Biotechnol 雑誌   104 ( 3 )   1135 - 1148   2020.02

Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. Reviewed

Kashiyama Y, Yokoyama A, Shiratori T, Hess S, Not F, Bachy C, Gutierrez-Rodriguez A, Kawahara J, Suzaki T, Nakazawa M, Ishikawa T, Maruyama M, Wang M, Chen M, Gong Y, Seto K, Kagami M, Hamamoto Y, Honda D, Umetani T, Shihongi A, Kayama M, Matsuda T, Taira J, Yabuki A, Tsuchiya M, Hirakawa Y, Kawaguchi A, Nomura M, Nakamura A, Namba N, Matsumoto M, Tanaka T, Yoshino T, Higuchi R, Yamamoto A, Maruyama T, Yamaguchi A, Uzuka A, Miyagishima S, Tanifuji G, Kawachi M, Kinoshita Y, Tamiaki H

The ISME journal   13 ( 8 )   2019.08

Anaerobic respiration coupled with mitochondrial fatty acid synthesis in wax ester fermentation by Euglena gracilis. Reviewed

Nakazawa M, Ando H, Nishimoto A, Ohta T, Sakamoto K, Ishikawa T, Ueda M, Sakamoto T, Nakano Y, Miyatake K, Inui H

FEBS letters   592 ( 24 )   2018.12

Gene cloning, expression, and X-ray crystallographic analysis of a β-mannanase from Eisenia fetida. Reviewed

Ueda M, Hirano Y, Fukuhara H, Naka Y, Nakazawa M, Sakamoto T, Ogata Y, Tamada T

Enzyme and microbial technology   117   2018.10

Crystal structure of exo-rhamnogalacturonan lyase from Penicillium chrysogenum as a member of polysaccharide lyase family 26. Reviewed

592 ( 8 )   2018.04

Cloning and expression of a chitinase gene from Eisenia fetida. Reviewed

Ueda M, Shioyama T, Nakadoi K, Nakazawa M, Sakamoto T, Iwamoto T, Sakaguchi M.

Int J Biol Macromol 雑誌   104 ( B )   2017.11

A novel α-galactosidase from Fusarium oxysporum and its application in determining the structure of the gum arabic side chain. Reviewed

Maruta A, Yamane M, Matsubara M, Suzuki S, Nakazawa M, Ueda M, Sakamoto T.

Enzyme Microb Technol 雑誌   103   2017.08

Physiological functions of pyruvate:NADP+ oxidoreductase and 2-oxoglutarate decarboxylase in Euglena gracilis under aerobic and anaerobic conditions. Reviewed

Nakazawa M, Hayashi R, Takenaka S, Inui H, Ishikawa T, Ueda M, Sakamoto T, Nakano Y, Miyatake K.

Biosci Biotechnol Biochem 雑誌   81 ( 7 )   2017.07

Identification of a novel Penicillium chrysogenum rhamnogalacturonan rhamnohydrolase and the first report of a rhamnogalacturonan rhamnohydrolase gene. Reviewed

Matsumoto S, Yamada H, Kunishige Y, Takenaka S, Nakazawa M, Ueda M, Sakamoto T

Enzyme Microb Technol 雑誌   98   76 - 85   2017.03

Analysis of carbohydrate binding module family 12 in a novel glycoside hydrolase family 23 chitinase (Ra-ChiC) from thermophilic Ralstonia sp. A-471

Chitin and Chitosan Research   23 ( 1 )   1 - 7   2017.01

C2 metabolism in Euglena.

Nakazawa M.

Adv Exp Med Biol 著書   979   2017

Critical Involvement of Environmental Carbon Dioxide Fixation to Drive Wax Ester Fermentation in Euglena.

Padermshoke A, Ogawa T, Nishio K, Nakazawa M, Nakamoto M, Okazawa A, Kanaya S, Arita M, Ohta D.

PLoS One 雑誌   11 ( 9 )   2016.09

Naringin lauroyl ester inhibits lipopolysaccharide-induced activation of nuclear factor κB signaling in macrophages.

Hattori H, Tsutsuki H, Nakazawa M, Ueda M, Ihara H, Sakamoto T.

Biosci Biotechnol Biochem 雑誌   80 ( 7 )   1403 - 1409   2016.07

Molecular characterization of a Penicillium chrysogenum exo-rhamnogalacturonan lyase that is structurally distinct from other polysaccharide lyase family proteins. Reviewed

Iwai M, Kawakami T, Ikemoto T, Fujiwara D, Takenaka S, Nakazawa M, Ueda M, Sakamoto T.

Appl Microbiol Biotechnol 雑誌   99 ( 20 )   8515 - 8525   2015.10

Identification and characterization of three Penicillium chrysogenum α-l-arabinofuranosidases (PcABF43B, PcABF51C, and AFQ1) with different specificities toward arabino-oligosaccharides. Reviewed

Shinozaki A, Hosokawa S, Nakazawa M, Ueda M, Sakamoto T.

Enzyme Microb Technol 雑誌   73-74   65 - 71   2015.06

Alteration of Wax Ester Content and Composition in Euglena gracilis with Gene Silencing of 3-ketoacyl-CoA Thiolase Isozymes.

Nakazawa M, Andoh H, Koyama K, Watanabe Y, Nakai T, Ueda M, Sakamoto T, Inui H, Nakano Y, Miyatake K.

Lipids 雑誌   50 ( 5 )   483 - 492   2015.04

Cloning and expression of the cold-adapted endo-1,4-β-glucanase gene from Eisenia fetida.

Ueda M, Ito A, Nakazawa M, Miyatake K, Sakaguchi M, Inouye K.

Carbohydr Polym 雑誌   101   511 - 516   2014.01

Characterization of a bifunctional glyoxylate cycle enzyme, malate synthase/isocitrate lyase, of Euglena gracilis. Reviewed

Nakazawa M, Nishimura M, Inoue K, Ueda M, Inui H, Nakano Y, Miyatake K.

J Eukaryot Microbiol 雑誌   58 ( 2 )   128 - 133   2011

Purification and characterization of a new serine protease (EF-SP2) with anti-plant viral activity from Eisenia foetida: Analysis of anti-plant viral activity of EF-SP2 Reviewed

Ueda M, Yamashita T, Nakazawa M, Miyatake K, Ohki S, Sakaguchi M, Inouye K

Process Biochemistry 雑誌   46 ( 9 )   1711 - 1716   2011

Purification and characterization of 1,3-β-D-glucanase from Eisenia foetida Reviewed

Ueda M, Yamaki K, Goto T, Nakazawa M, Miyatake K, Sakaguchi M, Inouye K

Carbohydrate Polymers 雑誌   86 ( 1 )   271 - 276   2011

Crystallization and preliminary X-ray diffraction studies of the catalytic domain of a novel chitinase, a member of GH family 23, from the moderately thermophilic bacterium Ralstonia sp. A-471. Reviewed

Okazaki N, Arimori T, Nakazawa M, Miyatake K, Ueda M, Tamada T.

Acta Crystallogr Sect F Struct Biol Cryst Commun 雑誌   67 ( 4 )   494 - 497   2011

A novel cold-adapted cellulase complex from Eisenia foetida: Characterization of a multienzyme complex with carboxymethylcellulase, beta-glucosidase, beta-1,3 glucanase, and beta-xylosidase. Reviewed

Ueda M, Goto T, Nakazawa M, Miyatake K, Sakaguchi M, Inouye K

Comp Biochem Physiol B Biochem Mol Biol 雑誌   157 ( 1 )   26 - 32   2010

A novel goose-type lysozyme gene with chitinolytic activity from the moderately thermophilic bacterium Ralstonia sp. A-471: cloning, sequencing, and expression. Reviewed

Ueda M, Ohata K, Konishi T, Sutrisno A, Okada H, Nakazawa M, Miyatake K

Appl Microbiol Biotechnol 雑誌   81 ( 6 )   1077 - 1085   2009.01

A novel anti-plant viral protein from coelomic fluid of the earthworm Eisenia foetida: purification, characterization and its identification as a serine protease. Reviewed

Ueda M, Noda K, Nakazawa M, Miyatake K, Ohki S, Sakaguchi M, Inouye K

Comp Biochem Physiol B Biochem Mol Biol 雑誌   151 ( 4 )   381 - 385   2008.12

Purification and characterization of novel raw-starch-digesting and cold-adapted alpha-amylases from Eisenia foetida Reviewed

Ueda M, Asano T, Nakazawa M, Miyatake K, Inouye K

Comp Biochem Physiol B Biochem Mol Biol 雑誌   150 ( 1 )   125 - 130   2008.05

Molecular characterization of a bifunctional glyoxylate cycle enzyme, malate synthase/isocitrate lyase, in Euglena gracilis. Reviewed

Nakazawa M, Minami T, Teramura K, Kumamoto S, Hanato S, Takenaka S, Ueda M, Inui H, Nakano Y, Miyatake K.

Comp Biochem Physiol B Biochem Mol Biol. 雑誌   141 ( 4 )   445 - 452   2005.08

Purification and characterization of chitinase B from moderately thermophilic bacterium Ralstonia sp. A-471. Reviewed

Ueda M, Kotani Y, Sutrisno A, Nakazawa M, Miyatake K.

Biosci Biotechnol Biochem 雑誌   69 ( 4 )   842 - 844   2005.04

A chitinase with high activity toward partially N-acetylated chitosan from a new, moderately thermophilic, chitin-degrading bacterium, Ralstonia sp. A-471. Reviewed

Sutrisno A, Ueda M, Abe Y, Nakazawa M, Miyatake K.

Appl Microbiol Biotechnol 雑誌   Vol.63 ( No. 4 )   398 - 406   2004.01

Effect of UV screens and preservatives on vitellogenin and choriogenin production in male medaka (Oryzias latipes). Reviewed

Inui M, Adachi T, Takenaka S, Inui H, Nakazawa M, Ueda M, Watanabe H, Mori C, Iguchi T, Miyatake K.

Toxicology 雑誌   Vol.194 ( No. 1-2 )   43 - 50   2003.12

Pyruvate:NADP+ oxidoreductase is stabilized by its cofactor, thiamin pyrophosphate, in mitochondria of Euglena gracilis. Reviewed

Nakazawa M, Takenaka S, Ueda M, Inui H, Nakano Y, Miyatake K.

Arch Biochem Biophys 雑誌   Vol.411 ( No. 2 )   183 - 188   2003.03

Occurrence of a novel NADP(+)-linked alcohol dehydrogenase in Euglena gracilis. Reviewed

Munir I, Nakazawa M, Harano K, Yamaji R, Inui H, Miyatake K, Nakano Y.

Comp Biochem Physiol B Biochem Mol Biol 雑誌   Vol.132 ( No. 3 )   535 - 540   2002.07

The origin of pyruvate: NADP+ oxidoreductase in mitochondria of Euglena gracilis. Reviewed

Nakazawa M, Inui H, Yamaji R, Yamamoto T, Takenaka S, Ueda M, Nakano Y, Miyatake K.

FEBS Letters 雑誌   Vol.479 ( No. 3 )   155 - 156   2000.08

細胞内電子伝達の改変による嫌気下物質生産向上を目指した研究 Domestic conference

佐々木晴紀、中澤昌美、柏山祐一郎、戸谷吉博、清水 浩、上田光宏、阪本龍司

第38回ユーグレナ研究会若手の会研究集会  2023.11  ユーグレナ研究会若手の会

ミミズ由来の低温適応性α-アミラーゼ(Ef-Amyll)の酵素機能向上に関する研究 Domestic conference

大西 爽太, 平野 優, 中澤 昌美, 阪本 龍司, 玉田 太郎, 上田 光宏

日本生化学会大会プログラム・講演要旨集  2023.10  (公社)日本生化学会

Development of anaerobic bioproduction by modification of electron transfer capability International conference

Haruki Sasaki

Euglena day, ULiege  2023.09  University of Liege

Analysis of anaerobic metabolism in Euglena gracilis International conference

Rikuto Oishi

Euglena day, ULiege  2023.09  University of Liege

Anaerobic wax ester production in Euglena gracilis International conference

Masami Nakazawa

Euglena day, ULiege  2023.09  University of Liege

Anaerobic Respiration Coupled with Wax Ester Production in Euglena gracilis Invited International conference

Masami Nakazawa

3rd Annual International Congress on Euglenoids 2023  2023.07  Euglena International Network

Wax ester production coupled with anaerobic respiration in Euglena gracilis International conference

Masami Nakazawa

ISEP Virtual Meeting 2023  2023.01  The International Society for Evolutionary Protistology

Gum arabicに作用するFusarium oxysporum由来3種のL-アラビノフラノシダーゼの反応特性解析 Domestic conference

井上 早希, 大前 晴香, 松尾 佳実, 近藤 辰哉, 中澤 昌美, 上田 光宏, 石渡 明弘, 伊藤 幸成, 阪本 龍司

応用糖質科学  2023  (一社)日本応用糖質科学会

キャッサバパルプペクチンの糖鎖構造解析 Domestic conference

小谷侑加、林孟宜、柴田望、中澤昌美、上田光宏、阪本龍司

日本応用糖質科学会大会  2022.09  (一社)日本応用糖質科学会

Rapaza viridisの盗葉緑体獲得過程におけるピレノイドの再構成メカニズム Domestic conference

丸山 萌、加賀本 剛、中澤 昌美、蘆田 弘樹、粟井 光一郎、柏山 祐一郎

日本植物学会第86回大会  2022.09  日本植物学会

広範な真核生物に保存された新規クロロフィル代謝遺伝子候補の機能解析 Domestic conference

柏山 祐一郎、中澤 昌美、谷藤 吾朗

日本植物学会第86回大会  2022.09  日本植物学会

皮膚常在菌叢改善を目的とした糖質資化性評価 International coauthorship Domestic conference

松末 海央, 飯田 聡史, 橋爪 雄志, Tandia Mahamadou, 中澤 昌美, 上田 光宏, 阪本 龍司

日本応用糖質科学会大会  2022.09  (一社)日本応用糖質科学会

ユーグレナワックスエステル合成系におけるミトコンドリア補酵素変換の重要性 Domestic conference

中澤 昌美、髙橋 夢月、柏山祐一郎、乾　博、上田 光宏、阪本 龍司

日本農芸化学会2022年度大会  2022.03 

アミスギタケの子実体形成におけるスレオニン添加効果 Domestic conference

上坂 友了, 中澤 昌美, 阪本 龍司, 岩本 武夫, 岸本 通雅, 上田 光宏

第94回日本生化学会大会  2021.11 

Aspergillus oryzae 由来アラビナン分解系遺伝子の大量発現系の構築とアラビノース生産への利用 Domestic conference

清水智菜美、中澤昌美、上田光宏、長谷川芳則、阪本龍司

日本応用糖質科学会　第47回近畿支部会  2021.11 

Aspergillus oryzae 由来2種のマンナンアセチルエステラーゼの機能解析とコンニャクゲル化への応用 Domestic conference

斉藤美穂、中澤昌美、上田光宏、長縄省吾、長谷川芳則、阪本龍司

日本応用糖質科学会　第47回近畿支部会  2021.11 

Anaerobic mitochondria in Euglenoids: metabolic and evolutionary aspects of anaerobic mitochondria. Invited International conference

Masami Nakazawa

1st Annual International Congress on Euglenoids  2021.11 

ユーグレナワックスエステル発酵における補酵素変換の重要性 Domestic conference

髙橋 夢月、中澤 昌美、乾　博、上田 光宏、阪本 龍司

ユーグレナ研究会第36回研究集会  2021.10 

ワックスエステル発酵制御因子WSRKがピルビン酸：NADP+ 酸化還元酵素のリン酸化および酵素活性におよぼす影響 Domestic conference

藏前 由衣、駒井 陽輔、石井 侑樹、小川 貴央、丸田 隆典、重岡　成、中澤 昌美、石川 孝博

ユーグレナ研究会第36回研究集会  2021.10 

ユーグレナのミトコンドリア脂肪酸合成系とロドキノンの関与について

乾 博, 中澤 昌美

ビタミン  2021.10  (公社)日本ビタミン学会

ユーグレナのミトコンドリア脂肪酸合成系とロドキノンの関与について

乾 博, 中澤 昌美

ビタミン  2021.10  (公社)日本ビタミン学会

電子伝達体キノンの構成改変による物質生産転換法の開発 Domestic conference

長谷川雅樹、 中澤 昌美、 坂元 君年、柏山祐一郎、上田 光宏、阪本 龍司

第31回イソプレノイド研究会  2021.09 

生化学的手法によるGum arabic の糖鎖構造解析 Domestic conference

松尾佳実、清水智菜美、吉條美由、近藤辰哉、中澤昌美、上田光宏、阪本龍司

第21回関西グライコサイエンスフォーラム  2021.05 

糸状菌由来ペクチンエステラーゼの反応特性とペクチン構造解析への応用 Domestic conference

東ほたる、中澤昌美、上田光宏、山田浩之、長谷川芳則、阪本龍司

日本応用糖質科学会第46回近畿支部会  2020.11 

Anaerobic ATP production coupled with fatty acid synthesis in Euglena mitochondria International conference

Masami Nakazawa

Euglena International Network inaugural meeting   2020.11  Euglena International Network

ロドキノンを介する嫌気的呼吸鎖と共役した長鎖脂肪酸合成系の解明 Domestic conference

中澤昌美、髙橋夢月、乾　博、坂元君年、上田光宏、阪本龍司

第30回イソプレノイド研究会  2020.09 

エキソ-β-1,3-ガラクタナーゼとβ-L-アラビノフラノシダーゼ遺伝子の異種発現系の構築とガムアラビックに対する反応性 Domestic conference

大前 晴香、高濱 里帆、中澤 昌美、上田 光宏、石渡 明弘、伊藤 幸成、藤田 清貴、阪本 龍司

2020年度日本応用糖質科学会 応用糖質科学シンポジウム  2020.09  日本応用糖質科学会

コエンザイムQの合成経路の多様性

Grant-in-Aid for Scientific Research(B)  2022

多様な栄養戦略を採る「増やしにくい生物」の増殖能強化による実験生物化への挑戦

Grant-in-Aid for Challenging Research (Pioneering)/(Exploratory)  2022

生物環境化学

2022   Weekly class   Undergraduate

Bioenviromental Chemistry

2021    

Laboratory : Chemistry of Biological Molecules

2021   Practical Training  

General Principles of Applied Biological Chemistry

2021    

Topics in Bioresource Cycle Engineering

2021    

「女子中高生のための関西科学塾」運営委員および実験講師

2016.04 - Now

女子中高生のための関西科学塾運営委員

Role(s)： Planner,　Logistic support,　Performer

2015 - Now