Updated on 2024/02/13

写真a

 
Harada Atsushi
 
Organization
Graduate School of Engineering Division of Science and Engineering for Materials, Chemistry and Biology Professor
School of Engineering Department of Applied Chemistry
Title
Professor
Affiliation
Institute of Engineering
Contact information
メールアドレス
Affiliation campus
Nakamozu Campus

Position

  • Graduate School of Engineering Division of Science and Engineering for Materials, Chemistry and Biology 

    Professor  2022.04 - Now

  • School of Engineering Department of Applied Chemistry 

    Professor  2022.04 - Now

Degree

  • 博士(工学) ( Others )

Research Areas

  • Life Science / Biomedical engineering

  • Life Science / Biomaterials

  • Nanotechnology/Materials / Polymer chemistry

Research Interests

  • ナノメディシン

  • polymer self-assembly

  • nano-biomaterials

  • polymer biomaterials

  • ドラッグデリバリーシステム

Research subject summary

  • バイオマテリアルとしての有機・無機ハイブリッド材料設計

  • ドラッグデリバリー用高分子材料設計

  • 遺伝子ベクター用高分子材料設計

Research Career

  • 低侵襲治療のための有機・無機ハイブリッド材料設計

  • ドラッグデリバリー用高分子自己組織体設計

  • 遺伝子ベクターとしての種々機能を組み込んだ高分子材料設計

Professional Memberships

  • 日本バイオマテリアル学会

    1998.10 - Now

  • 高分子学会

    1998.05 - Now

  • 日本化学会

    1998.05 - Now

  • 高分子学会

  • 日本バイオマテリアル学会

  • 日本化学会

  • KINKI CHEMICAL SOCIETY, JAPAN

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Awards

  • 平成21年度特別研究員等審査会専門委員(書面担当)表彰

    2010.07   日本学術振興会  

  • 優秀講演賞

    2007.04   日本化学会  

  • Japan-Korea Biomaterials Research Young Investigator’s Award

    2000.11   日本バイオマテリアル学会・韓国バイオマテリアル学会  

Job Career (off-campus)

  • Osaka Metropolitan University   Graduate School of Engineering Division of Science and Engineering for Materials, Chemistry and Biology

    2022.04 - Now

  • Osaka Prefecture University   Graduate School of Engineering

    2018.04 - Now

  • 京都大学 客員准教授

    2016.04 - 2017.03

  • 九州大学先導化学研究所 非常勤講師

    2013.04 - 2014.03

  • 神戸大学理学部 非常勤講師

    2005.04 - 2006.03

  • 東京大学大学院工学系研究科助手

    1998.05 - 2003.03

  • 日本学術振興会特別研究員(DC2)

    1998.04 - 1998.05

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Papers

  • Reversible Stabilization of Nanofiber-Polyplexes through Introducing Cross-Linkages

    Aono R.

    Journal of Functional Biomaterials   15 ( 1 )   2024.01( ISSN:2079-4983

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  • Preparing Size-Controlled Liposomes Modified with Polysaccharide Derivatives for pH-Responsive Drug Delivery Applications

    Shin Yanagihara, Yukiya Kitayama, Eiji Yuba, Atsushi Harada

    Life   13 ( 11 )   2158 - 2158   2023.11( ISSN:2075-1729

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    Publishing type:Research paper (scientific journal)  

    The liposome particle size is an important parameter because it strongly affects content release from liposomes as a result of different bilayer curvatures and lipid packing. Earlier, we developed pH-responsive polysaccharide-derivative-modified liposomes that induced content release from the liposomes under weakly acidic conditions. However, the liposome used in previous studies size was adjusted to 100–200 nm. The liposome size effects on their pH-responsive properties were unclear. For this study, we controlled the polysaccharide-derivative-modified liposome size by extrusion through polycarbonate membranes having different pore sizes. The obtained liposomes exhibited different average diameters, in which the diameters mostly corresponded to the pore sizes of polycarbonate membranes used for extrusion. The amounts of polysaccharide derivatives per lipid were identical irrespective of the liposome size. Introduction of cholesterol within the liposomal lipid components suppressed the size increase in these liposomes for at least three weeks. These liposomes were stable at neutral pH, whereas the content release from liposomes was induced at weakly acidic pH. Smaller liposomes exhibited highly acidic pH-responsive content release compared with those from large liposomes. However, liposomes with 50 mol% cholesterol were not able to induce content release even under acidic conditions. These results suggest that control of the liposome size and cholesterol content is important for preparing stable liposomes at physiological conditions and for preparing highly pH-responsive liposomes for drug delivery applications.

    DOI: 10.3390/life13112158

    PubMed

  • Inkjet-Based Intracellular Delivery System that Effectively Utilizes Cell-Penetrating Peptides for Cytosolic Introduction of Biomacromolecules through the Cell Membrane

    Mika Omura, Kenta Morimoto, Yurina Araki, Hisaaki Hirose, Yoshimasa Kawaguchi, Yukiya Kitayama, Yuto Goto, Atsushi Harada, Ikuo Fujii, Tomoka Takatani-Nakase, Shiroh Futaki, Ikuhiko Nakase

    ACS Applied Materials & Interfaces   15 ( 41 )   47855 - 47865   2023.10( ISSN:1944-8244

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/acsami.3c01650

    PubMed

  • pH-Responsive Capsules Fabricated by Interfacial Photo-Cross-Linking Utilizing the Photoreactivity and pH-Responsiveness of Thymine

    Yukiya Kitayama, Akali Dosaka, Atsushi Harada

    ACS Applied Polymer Materials   5 ( 9 )   7390 - 7399   2023.08( ISSN:2637-6105

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/acsapm.3c01283

  • Biocompatible Polymer-Grafted TiO<inf>2</inf> Nanoparticle Sonosensitizers Prepared Using Phosphonic Acid-Functionalized RAFT Agent

    Kitayama Y.

    Polymers   15 ( 11 )   2023.06

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  • Effect of Poly(Vinyl Alcohol) Concentration and Chain Length on Polymer Nanogel Formation in Aqueous Dispersion Polymerization

    Yukiya Kitayama, Shunsuke Takigawa, Atsushi Harada

    Molecules   28 ( 8 )   3493 - 3493   2023.04

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    Publishing type:Research paper (scientific journal)  

    Nanotechnology has attracted increasing interest in various research fields for fabricating functional nanomaterials. In this study, we investigated the effect of poly(vinyl alcohol) (PVA) addition on the formation and thermoresponsive properties of poly(N-isopropyl acrylamide)-based nanogels in aqueous dispersion polymerizations. During dispersion polymerization, PVA appears to play three roles: (i) it bridges the generated polymer chains during polymerization, (ii) it stabilizes the formed polymer nanogels, and (iii) it regulates the thermoresponsive properties of the polymer nanogels. By regulating the bridging effect of PVA via changing the PVA concentration and chain length, the size of the obtained polymer gel particles was maintained in the nanometer range. Furthermore, we found that the clouding-point temperature increased when using low-molecular weight PVA. We believe that the knowledge gained in this study regarding the effect of PVA concentration and chain length on nanogel formation will aid in the future fabrication of functional polymer nanogels.

    DOI: 10.3390/molecules28083493

    PubMed

  • Reversible complexation mediated polymerization of methacrylates using amine catalysts in aqueous heterogeneous systems

    Kitayama Y.

    Polymer Journal   55 ( 1 )   1 - 12   2023.01( ISSN:00323896

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  • Cationic lipid potentiated the adjuvanticity of polysaccharide derivative-modified liposome vaccines.

    Eiji Yuba, Yuna Kado, Nozomi Kasho, Atsushi Harada

    Journal of controlled release : official journal of the Controlled Release Society   362   767 - 776   2022.10( ISSN:01683659

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Antigen carriers that can selectively deliver antigens to antigen presenting cells and which can simultaneously activate these cells (adjuvant property) are necessary for efficient cancer immunotherapy or vaccination. Delivery of a model antigen into dendritic cell cytosol has been achieved by pH-responsive polymer-modified liposomes via destabilization of endosomal membranes responding to acidic pH, which impelled antigen-specific cellular immunity. Furthermore, β-glucan-based pH-responsive polysaccharides have shown not only cytosolic antigen delivery performance but also adjuvant property, which further heightened cellular immune responses. Because pH-responsive polysaccharides have anionic carboxy groups, cationic lipid was introduced to liposomes in this study to improve the modification efficiency of pH-responsive polysaccharides and to improve their adjuvanticity and immunity-inducing functions. Introduction of cationic lipids increased the amounts of polysaccharide derivatives on the liposome and increased the cellular association of the liposomes to dendritic cells. Liposomes containing β-glucan-based pH-responsive polysaccharides and cationic lipids increased cytokine production from dendritic cells much more than other polysaccharide derivatives did. Furthermore, through improvement of intra-tumoral immunosuppression and induction of antigen-specific cellular immunity, administering these liposomes impelled tumor suppression even with a small antigen dose. These results suggest that introducing cationic lipids and using pH-responsive polysaccharides having intrinsically adjuvant function are effective for producing liposomal nanovaccines showing strong immunity-inducing function.

    DOI: 10.1016/j.jconrel.2022.10.016

    PubMed

  • Reversible chain transfer catalyzed polymerization in miniemulsion systems with tetraiodomethane as a catalyst

    Kitayama Y.

    Polymer Chemistry   13 ( 44 )   6179 - 6186   2022.09( ISSN:17599954

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  • Programed Thermoresponsive Polymers with Cleavage-Induced Phase Transition Reviewed

    Yukiya Kitayama, Yasumichi Yazaki, Junya Emoto, Eiji Yuba, Atsushi Harada

    Molecules   27 ( 18 )   6082   2022.09

  • Thermoresponsiveness of Carboxylated Polyallylamines Induced by Divalent Counterions as Ionic Effectors

    Junya Emoto, Yukiya Kitayama, Atsushi Harada

    Macromolecules   2022.07( ISSN:0024-9297

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/acs.macromol.2c00795

  • Systematic study on fabrication of hollow poly(vinyl cinnamate) particles by particulate interfacial photocrosslinking

    Yukiya Kitayama, Atsushi Harada

    Aggregate   3 ( 6 )   2022.06( ISSN:2692-4560

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/agt2.251

    Other URL: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/agt2.251

  • Carboxy-functionalized pH responsive capsule polymer particles fabricated by particulate interfacial photocrosslinking

    Yukiya Kitayama, Atsushi Harada

    Journal of Materials Chemistry B   10 ( 37 )   7570 - 7580   2022.03( ISSN:2050-750X

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    Publishing type:Research paper (scientific journal)  

    Carboxy-functionalized capsule polymer particles possessing neutral-alkali pH responsive controlled release capability were newly fabricated by interfacial photocrosslinking of spherical photoreactive polymer particles.

    DOI: 10.1039/d1tb02866a

    PubMed

  • Interfacial photocrosslinking of polymer particles possessing nucleobase photoreactive groups for hollow/capsule polymer fabrication

    Yukiya Kitayama, Akali Dosaka, Atsushi Harada

    Polymer Chemistry   13 ( 6 )   748 - 758   2022.01( ISSN:1759-9954

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    Publishing type:Research paper (scientific journal)  

    Herein, polystyrene-based particles possessing nucleobases in polymer side chains were prepared and nucleobase groups were applied to the interfacial photocrosslinking as photoreactive groups for the first time for fabricating hollow/capsule particles.

    DOI: 10.1039/d1py01438b

  • Interfacial Photocrosslinking of Polymer Particles Possessing Nucleobase Photoreactive Groups for Hollow/Capsule Fabrication Reviewed

    Yukiya Kitayama, Akali Dosaka, Atsushi Harada

    Polymer Chemistry 雑誌   in press   2022.01

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    Kind of work:Joint Work  

  • pH-Sensitive branched β-glucan-modified liposomes for activation of antigen presenting cells and induction of antitumor immunity Reviewed

    Shin Yanagihara, Nozomi Kasho, Koichi Sasaki, Naoto Shironaka, Yukiya Kitayama, Eiji Yuba, Atsushi Harada

    Journal of Materials Chemistry B 雑誌   9 ( 37 )   7713 - 7724   2021.10

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    Kind of work:Joint Work  

  • pH-Sensitive branched β-glucan-modified liposomes for activation of antigen presenting cells and induction of antitumor immunity.

    Shin Yanagihara, Nozomi Kasho, Koichi Sasaki, Naoto Shironaka, Yukiya Kitayama, Eiji Yuba, Atsushi Harada

    Journal of materials chemistry. B   9 ( 37 )   7713 - 7724   2021.09( ISSN:2050-750X

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Induction of cellular immunity is important for effective cancer immunotherapy. Although various antigen carriers for cancer immunotherapy have been developed to date, balancing efficient antigen delivery to antigen presenting cells (APCs) and their activation via innate immune receptors, both of which are crucially important for the induction of strong cellular immunity, remains challenging. For this study, branched β-glucan was selected as an intrinsically immunity-stimulating and biocompatible material. It was engineered to develop multifunctional liposomal cancer vaccines capable of efficient interactions with APCs and subsequent activation of the cells. Hydroxy groups of branched β-glucan (Aquaβ) were modified with 3-methylglutaric acid ester and decyl groups, respectively, to provide pH-sensitivity and anchoring capability to the liposomal membrane. The modification efficiency of Aquaβ derivatives to the liposomes was significantly high compared with linear β-glucan (curdlan) derivatives. Aquaβ derivative-modified liposomes released their contents in response to weakly acidic pH. As a model antigenic protein, ovalbumin (OVA)-loaded liposomes modified with Aquaβ derivatives interacted efficiently with dendritic cells, and induced inflammatory cytokine secretion from the cells. Subcutaneous administration of Aquaβ derivative-modified liposomes suppressed the growth of the E.G7-OVA tumor significantly compared with curdlan derivative-modified liposomes. Aquaβ derivative-modified liposomes induced the increase of CD8+ T cells, and polarized macrophages to the antitumor M1-phenotype within the tumor microenvironment. Therefore, pH-sensitive Aquaβ derivatives can be promising materials for liposomal antigen delivery systems to induce antitumor immune responses efficiently.

    DOI: 10.1039/d1tb00786f

    PubMed

  • Multifunctional Traceable Liposomes with Temperature-Triggered Drug Release and Neovasculature-Targeting Properties for Improved Cancer Chemotherapy.

    Eiji Yuba, Munenobu Takashima, Takaaki Hayashi, Daisuke Kokuryo, Ichio Aoki, Atsushi Harada, Sadahito Aoshima, Uma Maheswari Krishnan, Kenji Kono

    Molecular pharmaceutics   18 ( 9 )   3342 - 3351   2021.09( ISSN:1543-8384

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Poor distribution of nanocarriers at the tumor site and insufficient drug penetration into the tissue are major challenges in the development of effective and safe cancer therapy. Here, we aim to enhance the therapeutic effect of liposomes by accumulating doxorubicin-loaded liposomes at high concentrations in and around the tumor, followed by heat-triggered drug release to facilitate low-molecular-weight drug penetration throughout the tumor. A cyclic RGD peptide (cRGD) was incorporated into liposomes decorated with a thermosensitive polymer that allowed precise tuning of drug release temperature (i.e., Polymer-lip) to develop a targeted thermosensitive liposome (cRGD-Polymer-lip). Compared with conventional thermosensitive liposomes, cRGD-Polymer-lip enhanced the binding of liposomes to endothelial cells, leading to their accumulation at the tumor site upon intravenous administration in tumor-bearing mice. Drug release triggered by local heating strongly inhibited tumor growth. Notably, tumor remission was achieved via multiple administrations of cRGD-Polymer-lip and heat treatments. Thus, combining the advantages of tumor neovascular targeting and heat-triggered drug release, these liposomes offer high potential for minimally invasive and effective cancer chemotherapy.

    DOI: 10.1021/acs.molpharmaceut.1c00263

    PubMed

  • pH-Responsive Capsule Polymer Particles Prepared by Interfacial Photo-Cross-Linking: Effect of the Alkyl Chain Length of the pH-Responsive Monomer

    Yukiya Kitayama, Atsushi Harada

    ACS Applied Materials & Interfaces   13 ( 29 )   34973 - 34983   2021.07( ISSN:1944-8244

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/acsami.1c09203

    PubMed

  • pH-Responsive Capsule Polymer Particles Prepared by Interfacial Photo-Cross-Linking: Effect of the Alkyl Chain Length of the pH-Responsive Monomer Reviewed

    Yukiya Kitayama, Atsushi Harada

    ACS Applied Materials & Interfaces 雑誌   13 ( 29 )   34973 - 34983   2021.07

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    Kind of work:Joint Work  

  • Fabrication of gold nanohybrids modified with antibody and functional dendrimers for targeted photothermal theranostics

    Herlan Setiawan, Eiji Yuba, Atsushi Harada, Ichio Aoki, Kenji Kono

    Nano Select   2 ( 4 )   779 - 790   2021.04( ISSN:2688-4011

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/nano.202000218

    Other URL: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/nano.202000218

  • Hydrophilic Hyperbranched Polymer-Coated siRNA/Polyamidoamine Dendron-Bearing Lipid Complexes Preparation for High Colloidal Stability and Efficient RNAi.

    Eiji Yuba, Takashi Korenaga, Atsushi Harada

    Bioconjugate chemistry   32 ( 3 )   563 - 571   2021.03( ISSN:1043-1802

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    RNA interference (RNAi) using siRNA has gained much attention for use in therapies for cancer and genetic disorders. To establish RNAi-based therapeutics, the development of efficient siRNA nanocarriers is desired. Earlier, we developed polyamidoamine dendron-bearing lipids able to form complexes with nucleic acids as gene vectors. Especially, dendron lipids with unsaturated alkyl chains (DL-G1-U2) induced efficient endosomal escape by membrane fusion, leading to efficient transfection in vitro. For this study, dendron lipids having oleyl/linoleyl groups (DL-G1-U3) were designed to increase membrane fusogenic activity further. Indeed, DL-G1-U3/siRNA complexes achieved higher membrane fusogenic activity and knockdown of the target gene more efficiently than conventional DL-G1-U2/siRNA complexes did. A hydrophilic polymer, hyperbranched polyglycidol lauryl ester (HPG-Lau), was modified further on the surface of DL-G1-U3/siRNA complexes to provide colloidal stability. Surface modification of HPG-Lau increased the colloidal stability in a physiological condition more than complexes without HPG-Lau. Importantly, HPG-Lau-coated DL/siRNA complexes showed identical RNAi effects to those of parental DL/siRNA complexes, whereas the RNAi activity of poly(ethylene glycol)-bearing lipid (PEG-PE)-modified DL/siRNA complexes was hindered completely. Introduction of unsaturated bonds into dendron lipids and selection of suitable hydrophilic polymers for nanocarrier modification are important for obtaining efficient siRNA vectors toward in vivo siRNA delivery.

    DOI: 10.1021/acs.bioconjchem.1c00035

    PubMed

  • Interfacial Photo-Cross-Linking: Simple but Powerful Approach for Fabricating Capsule Polymer Particles with Tunable pH-Responsive Controlled Release Capability.

    Kitayama Y, Harada A

    ACS applied materials & interfaces   13 ( 8 )   10359 - 10375   2021.03( ISSN:1944-8244

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  • Hydrophilic hyperbranched polymer-coated siRNA/polyamidoamine dendron-bearing lipid complexes preparation for high colloidal stability and efficient RNAi Reviewed

    E. Yuba, T. Korenaga, A. Harada

    Bioconjug. Chem. 雑誌   32 ( 3 )   563 - 571   2021.03

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    Kind of work:Joint Work  

  • Hydrophilic hyperbranched polymer-coated siRNA/polyamidoamine dendron-bearing lipid complexes preparation for high colloidal stability and efficient RNAi Reviewed

    E. Yuba, T. Korenaga, A. Harada

    Bioconjug. Chem. 雑誌   32 ( 3 )   563 - 571   2021.03

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    Kind of work:Joint Work  

  • Interfacial photo-cross-linking: simple but powerful approach for fabricating capsule polymer particles with tunable pH-responsive controlled release capability Reviewed

    Y. Kitayama, H. Harada

    ACS Appl. Mater. Interface 雑誌   13 ( 8 )   10359 - 10375   2021.02

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    Kind of work:Joint Work  

  • Interfacial photo-cross-linking: simple but powerful approach for fabricating capsule polymer particles with tunable pH-responsive controlled release capability Reviewed

    Y. Kitayama, H. Harada

    ACS Appl. Mater. Interface 雑誌   13 ( 8 )   10359 - 10375   2021.02

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    Kind of work:Joint Work  

  • Development of Mannose-Modified Carboxylated Curdlan-Coated Liposomes for Antigen Presenting Cell Targeted Antigen Delivery

    Eiji Yuba, Yoshiki Fukaya, Shin Yanagihara, Nozomi Kasho, Atsushi Harada

    Pharmaceutics   12 ( 8 )   754 - 754   2020.08

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    Specific delivery to antigen presenting cells (APC) and precise control of the intracellular fate of antigens are crucial to induce cellular immunity that directly and specifically attacks cancer cells. We previously achieved cytoplasmic delivery of antigen and activation of APC using carboxylated curdlan-modified liposomes, which led to the induction of cellular immunity in vivo. APCs express mannose receptors on their surface to recognize pathogen specifically and promote cross-presentation of antigen. In this study, mannose-residue was additionally introduced to carboxylated curdlan as a targeting moiety to APC for further improvement of polysaccharide-based antigen carriers. Mannose-modified curdlan derivatives were synthesized by the condensation between amino group-introduced mannose and carboxy group in pH-sensitive curdlan. Mannose residue-introduced carboxylated curdlan-modified liposomes showed higher pH-sensitivity than that of liposomes modified with conventional carboxylated curdlan. The introduction of mannose-residue to the liposomes induced aggregation in the presence of Concanavalin A, indicating that mannose residues were presented onto liposome surface. Mannose residue-introduced carboxylated curdlan-modified liposomes exhibited high and selective cellular association to APC. Furthermore, mannose residue-introduced carboxylated curdlan-modified liposomes promoted cross-presentation of antigen and induced strong antitumor effects on tumor-bearing mice. Therefore, these liposomes are promising as APC-specific antigen delivery systems for the induction of antigen-specific cellular immunity.

    DOI: 10.3390/pharmaceutics12080754

    PubMed

  • Development of mannose-modified carboxylated curdlan-coated liposomes for antigen presenting cell targeted antigen delivery Reviewed

    E. Yuba, Y. Fukaya, S. Yanagihara, N. Kasho, A. Harada

    Pharmaceutics 雑誌   12   2020.08

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    Kind of work:Joint Work  

  • Comparison of In Vitro Performances of Nanorod and Nanofiber Polyplexes Prepared from Plasmid DNA and Poly(L-lysine) Terminally Bearing Multi-Arm PEG Reviewed

    R. Aono, K. Nomura, E. Yuba, A. Harada

    ACS Symposium Series   1350   13 - 21   2020.05

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    Kind of work:Joint Work  

  • Preparation of photothermal-chemotherapy nanohybrids by complexation of gold nanorods with polyamidoamine dendrimers having poly(ethylene glycol) and hydrophobic chains. Reviewed

    Takuya Hashimoto, Eiji Yuba, Atsushi Harada, Kenji Kono

    Journal of materials chemistry. B   8 ( 14 )   2826 - 2833   2020.04( ISSN:2050-750X

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    The combination of anticancer drugs and laser hyperthermia could lead to efficient cancer treatment with less-adverse effects. This study combined anticancer drug-loaded functional dendrimers and light-responsive gold nanorods to fabricate nanohybrids that can provide anticancer-drug delivery and subsequent heat generation under near-infrared laser irradiation. A condensation reaction was used to conjugate poly(ethylene glycol)-modified polyamidoamine dendrimers to carboxylated gold nanorod surfaces. Oleoyl groups were incorporated into dendrimers to improve the drug loading capacity. Doxorubicin loading capacity was improved by incorporation of oleoyl chains into dendrimers in the nanohybrid, indicating increased hydrophobic interaction between anticancer drugs and nanohybrids. The nanohybrids exhibited heat generation properties under near infrared laser irradiation. They released anticancer drugs over time. The combination of doxorubicin-loaded nanohybrids and laser irradiation showed markedly better cytotoxicity than that of the nanohybrids used with lasers and drug-loaded nanohybrids without the use of lasers. After intravenous or intratumoral injection of nanohybrids to tumor-bearing mice, a sharp temperature increase was observed at the tumor site under laser irradiation. Especially, intratumorally injected doxorubicin-loaded nanohybrids showed almost complete tumor growth suppression under laser irradiation. The results demonstrate that functional dendrimer-gold nanorod nanohybrids are promising as multi-functional nanomaterials to achieve synergistic effects of anticancer drugs and heat ablation to support effective cancer treatments.

    DOI: 10.1039/c9tb02163a

    PubMed

  • Temperature-Responsive Molecular Assemblies Using Oligo(Ethylene Glycol)-Attached Polyamidoamine Dendron Lipids and their Functions as Drug Carriers. Reviewed

    Takuya Hashimoto, Yuji Hirai, Eiji Yuba, Atsushi Harada, Kenji Kono

    Journal of functional biomaterials   11 ( 1 )   2020.03

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Temperature-responsive nanocarrier systems using external stimuli are one of the most widely investigated stimuli-responsive strategies because heat is easy and safe to use for hyperthermia and controlled drug delivery. Polyamidoamine dendron lipids (PAMAM-DLs) composed of PAMAM dendron as head group and two alkyl chains can exhibit temperature-responsive morphological change through the attachment of suitable moieties to terminal of PAMAM dendron. In this study, oligo(ethylene glycol)s including ethoxy- or methoxy-diethylene glycols were attached to the terminals of PAMAM-DL, and temperature-responsive properties of their self-assemblies were evaluated by calorimetric and turbidity measurements. In the evaluation of temperature-responsive properties, ethoxy diethylene glycol (EDEG)-attached PAMAM-DL composed of two saturated alkyl chains and PAMAM dendron with 1st generation had lipid bilayer structure and suitable cloud point for the application as drug carrier. In vitro performances of the assemblies combining EDEG-attached PAMAM-DLs with cholesteryl-oxy-poly(ethylene glycol) (PEG-Chol) was evaluated using doxorubicin (DOX) as an anticancer drug. Cellular uptake of DOX-loaded EDEG-attached PAMAM-DL/PEG-Chol assemblies was promoted at 42 °C rather than 37 °C, resulting in an effective decrease in cell viability.

    DOI: 10.3390/jfb11010016

    PubMed

  • Light-Activatable Transfection System Using Hybrid Vectors Composed of Thermosensitive Dendron Lipids and Gold Nanorods. Reviewed

    Takuya Hashimoto, Tomoya Hirata, Eiji Yuba, Atsushi Harada, Kenji Kono

    Pharmaceutics   12 ( 3 )   2020.03

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    BACKGROUND: Gene delivery to target cells is crucially important to establish gene therapy and regenerative medicine. Although various virus-based and synthetic molecule-based gene vectors have been developed to date, selective transfection in a site or a cell level is still challenging. For this study, both light-responsive and temperature-responsive synthetic gene vectors were designed for spatiotemporal control of a transfection system. METHODS: 11-Mercaptoundecanoic acid-coated gold nanorods were mixed with polyamidoamine dendron-bearing lipids of two types having amino-terminus or ethoxydiethylene glycol-terminus to obtain hybrid vectors. Hybrid vectors were mixed further with pDNA. Then we investigated their physicochemical properties and transfection efficacy with or without near infrared laser irradiation. RESULTS: Hybrid vectors formed complexes with pDNA and exhibited enhanced photothermal property under near infrared laser irradiation compared with parent gold nanorods. Transfection efficacy of complexes was promoted considerably by brief laser irradiation soon after complex application to the cells. Analysis of intracellular distribution revealed that laser irradiation promoted the adsorption of complexes to the cells and cytosolic release of pDNA, which is derived from the change in surface hydrophobicity of complexes through dehydration of temperature-responsive groups. CONCLUSIONS: Hybrid vector is promising as a light-activatable transfection system.

    DOI: 10.3390/pharmaceutics12030239

    PubMed

  • Photothermal-chemotherapy nanohybrid preparation by complexation of gold nanorods with polyamidoamine dendrimers having poly(ethylene glycol) and hydrophobic chains Reviewed

    T. Hashimoto, E. Yuba, A. Harada, K. Kono

    J. Mater. Chem. B 雑誌   8   2826 - 2833   2020.03

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    Kind of work:Joint Work  

  • Preparation of photothermal-chemotherapy nanohybrids by complexation of gold nanorods with polyamidoamine dendrimers having poly(ethylene glycol) and hydrophobic chains Reviewed

    T. Hashimoto, E. Yuba, A. Harada, K. Kono

    J. Mater. Chem. B 雑誌   8 ( 14 )   2826 - 2833   2020.03

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    Kind of work:Joint Work  

  • Preparation of photothermal-chemotherapy nanohybrids by complexation of gold nanorods with polyamidoamine dendrimers having poly(ethylene glycol) and hydrophobic chains Reviewed

    T. Hashimoto, E. Yuba, A. Harada, K. Kono

    J. Mater. Chem. B 雑誌   8 ( 14 )   2826 - 2833   2020.03

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    Kind of work:Joint Work  

  • Temperature-responsive molecular assemblies using oligo(ethylene glycol)-attached polyamidoamine dendron lipids and their functions as drug carriers Reviewed

    T. Hashimoto, Y. Hirai, E. Yuba, A. Harada, K. Kono

    J. Funct. Biomater. 雑誌   11   2020.03

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    Kind of work:Joint Work  

  • Light-activatable transfection system using hybrid vectors composed of thermosensitive dendron lipids and gold nanorods Reviewed

    T. Hashimoto, T. Hirata, E. Yuba, A. Harada, K. Kono

    Pharmaceutics 雑誌   12   2020.03

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    Kind of work:Joint Work  

  • Comparison of in Vitro Performances of Nanorod and Nanofiber Polyplexes Prepared from Plasmid DNA and Poly(L-lysine) Terminally Bearing Multi-Arm PEG

    Ryuta Aono, Kenta Nomura, Eiji Yuba, Atsushi Harada

    ACS Symposium Series   1350   13 - 21   2020( ISSN:0097-6156

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    Publishing type:Part of collection (book)  

    Poly(L-lysine)-bearing multi-arm poly(ethylene glycol) terminary (maPEG-PLL) can form nanorod and nanofiber polyplexes with plasmid DNA (pDNA) depending on the size (exclusion volume) of maPEG. Nanorod and nanofiber polyplexes were prepared using maPEG-PLL bearing different maPEG sizes. Gene expression of nanofiber and nanorod polyplexes was comparable, although the cellular uptake of nanofiber polyplexes was less than that of nanorod polyplexes. This suggests that there is a difference in cellular activities after polyplex enters the cell. The release of pDNA from polyplexes through the exchange with heparin occurred more easily in nanorod polyplexes than it did in nanofiber polyplexes. However, it was confirmed by real-time PCR measurements that pDNA molecules in nanofiber polyplexes exhibit high reactivity with polymerase compared with that in nanorod polyplexes. Further, higher reactivity of pDNA with polymerase in nanofiber polyplexes was maintained even in the presence of crowding cosolutes including Ficoll and dextran. This suggests that pDNA in nanofiber polyplex might reflect a higher efficiency of transcription process in the cell compared with pDNA in nanorod polyplex. These results provide valuable information in the design of nonviral gene vectors using cationic polymers.

    DOI: 10.1021/bk-2020-1350.ch002

  • Development of pH-Responsive Hyaluronic Acid-Based Antigen Carriers for Induction of Antigen-Specific Cellular Immune Responses.

    Maiko Miyazaki, Eiji Yuba, Hiroshi Hayashi, Atsushi Harada, Kenji Kono

    ACS biomaterials science & engineering   5 ( 11 )   5790 - 5797   2019.11

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Cancer immunotherapy has gained much attention because of the recent success of immune checkpoint inhibitors. Nevertheless, clinical therapeutic effects of immune checkpoint inhibitors remain limited, probably because most patients have other immune checkpoint molecules or because they lack cancer-specific cytotoxic T lymphocytes. Induction of cancer-specific cytotoxic T lymphocytes requires efficient antigen delivery systems that can convey cancer antigens specifically to antigen presenting cells, promote the endosomal escape of antigen into cytosol, and activate immune cells. Earlier, we reported cytoplasmic delivery systems of antigen using pH-sensitive polymer-modified liposomes. Adjuvant molecules were further incorporated into these liposomes to provide activation properties of cellular immune responses. This study further introduced cell specificity to these liposomal systems using hyaluronic acid-based pH-sensitive polymers, which are recognized by CD44 expressing on antigen presenting cells. pH-Sensitive hyaluronic acid derivative-modified liposomes showed much higher cellular association to antigen presenting cells than to fibroblasts with less CD44 expression. These liposomes achieved the delivery of model antigenic proteins into cytosol of dendritic cells and promoted Th1 cytokine production from the cells. Subcutaneous administration of these liposomes to mice induced antigen-specific cellular immune response in the spleen, leading to tumor regression in tumor-bearing mice. The results show that pH-sensitive hyaluronic acid derivative-modified liposomes are promising as multifunctional antigen carriers having cell-specificity, cytoplasmic antigen delivery performance, and adjuvant property to induce antigen-specific cellular immunity.

    DOI: 10.1021/acsbiomaterials.9b01278

    PubMed

  • Gene Expression of Aspect Ratio-Controlled Polyplexes Based on the Effect of Multi-Arm Poly(ethylene glycol).

    Atsushi Harada, Kenta Nomura, Eiji Yuba, Kenji Kono

    ACS biomaterials science & engineering   5 ( 11 )   5681 - 5687   2019.11

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Controlling the aspect ratio of polyplexes prepared by mixing pDNA with a polycation mixture of a poly(l-lysine) (PLL) homopolymer and PLL terminally bearing a multiarm poly(ethylene glycol) (PEG) part (maPEG-PLL) was examined. By varying the maPEG-PLL content in the polycation mixture, the condensation of pDNA accompanied by polyplex formation and the morphology of the polyplexes were evaluated by a dye exclusion assay and AFM observations, respectively. Increasing the maPEG-PLL content caused elongation of the polyplex, and polyplexes with aspect ratios from 2 to 10 were prepared successfully by controlling the maPEG-PLL content. The reactivity of pDNA in the polyplexes with varying aspect ratios against DNase I and polymerase were examined by agarose gel electrophoresis and real-time PCR measurements, respectively. Moreover, cellular uptake and transfection efficiency of the polyplexes by HeLa cells was evaluated. The results revealed that an increase in aspect ratio of the polyplexes caused an increase in PCR efficiency with a concomitant decrease in cellular uptake.

    DOI: 10.1021/acsbiomaterials.8b01498

    PubMed

  • Development of pH-responsive hyaluronic acid-based antigen carriers for induction of antigen-specific cellular immune responses Reviewed

    M. Miyazaki, E. Yuba, H. Hayashi, A. Harada, K. Kono

    ACS Biomaterials Science & Engineering 雑誌   5 ( 11 )   5790 - 5797   2019.10

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    Kind of work:Joint Work  

  • Sustained expression of HeyL is critical for the proliferation of muscle stem cells in overloaded muscle

    Fukuda S.

    eLife   8   2019.09

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  • Chondroitin Sulfate-Based pH-Sensitive Polymer-Modified Liposomes for Intracellular Antigen Delivery and Induction of Cancer Immunity. Reviewed

    Okubo M, Miyazaki M, Yuba E, Harada A

    Bioconjugate chemistry   30 ( 5 )   1518 - 1529   2019.05( ISSN:1043-1802

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Induction of cancer-specific cytotoxic T lymphocytes is crucially important to complement therapeutic effects of immune checkpoint inhibitors and to achieve efficient cancer immunotherapy. To induce cancer-specific cytotoxic T lymphocytes, cancer antigen carriers must have multiple functions to deliver cancer antigens to antigen presenting cells, release antigens into cytosol, and promote the maturation of these cells. We earlier achieved cytosolic delivery of antigens and induction of antigen-specific cytotoxic T lymphocytes using carboxylated polyglycidol or polysaccharide derivative-modified liposomes that can induce membrane fusion with endosomes in response to weakly acidic pH. Furthermore, pH-sensitivity and adjuvant properties of these polymers were enhanced strongly by introduction of hydrophobic carboxylated units to dextran. Against our expectations, these polymer-modified liposomes only slightly induce cancer immunity, probably because of the high hydrophobicity of spacer units. This study used a polysaccharide with charged groups (chondroitin sulfate) instead of dextran as a backbone to reduce hydrophobicity. Chondroitin sulfate derivative-modified liposomes showed almost equal pH-sensitivity to that of dextran derivative-modified liposomes and achieved selective delivery to dendritic cells, whereas dextran derivative-modified liposomes were highly taken up by both dendritic cells and fibroblasts. Chondroitin sulfate derivative-modified liposomes delivered model antigenic proteins into cytosol of dendritic cells and promoted cytokine production from the cells, leading to tumor regression on tumor-bearing mice after subcutaneous administration. Results demonstrate that charged groups having polysaccharide as a backbone can be used in an effective strategy to balance strong hydrophobicity of spacer units with their utilization for immunity-inducing systems.

    DOI: 10.1021/acs.bioconjchem.9b00221

    PubMed

  • Sonodynamic therapeutic effects of sonosensitizers with different intracellular distribution delivered by hollow nanocapsules exhibiting cytosol specific release Reviewed

    R. Teranishi, T. Matsuda, E. Yuba, K. Kono, A. Harada

    Macromolecular Bioscience 雑誌   19 ( 4 )   2019.04

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    Kind of work:Joint Work  

  • Chondroitin sulfate-based pH-sensitive polymer-modified liposomes for intracellular antigen delivery and induction of cancer immunity Reviewed

    M. Okubo, M. Miyazaki, E. Yuba, A. Harada

    Bioconjugate Chemistry 雑誌   30 ( 5 )   1518 - 1529   2019.04

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    Kind of work:Joint Work  

  • Emerging paradigms in nanotechnology for imaging and treatment of cerebral ischemia Reviewed

    S. Kaviarasi, E. Yuba, A. Harada, U. M. Krishnan

    J. Control. Release 雑誌   300 ( 1 )   22 - 45   2019.04

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    Kind of work:Joint Work  

  • Sonodynamic Therapeutic Effects of Sonosensitizers with Different Intracellular Distribution Delivered by Hollow Nanocapsules Exhibiting Cytosol Specific Release. Reviewed

    Teranishi R, Matsuda T, Yuba E, Kono K, Harada A

    Macromolecular bioscience   19 ( 4 )   e1800365   2019.04( ISSN:1616-5187

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  • Synthesis, characterization, and biomedical applications of an alkylated quercetin–gadolinium complex Reviewed

    S. Kaviarasi, K. S. S. Devi, P. Vinoth, V. Sridharan, E. Yuba, A. Harada, U. M. Krishnan

    ACS Biomaterials Science & Engineering 雑誌   5 ( 3 )   1215 - 1227   2019.03

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    Kind of work:Joint Work  

  • Gene expression of aspect ratio-controlled polyplexes based on the effect of multi-arm poly(ethylene glycol) Reviewed

    A. Harada, K. Nomura, E. Yuba, K. Kono

    ACS Biomaterials Science & Engineering 雑誌   5 ( 11 )   5681 - 5687   2019.03

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    Kind of work:Joint Work  

  • Synthesis, Characterization, and Biomedical Applications of an Alkylated Quercetin-Gadolinium Complex Reviewed

    Kayiarasi Sathyasivam, Devi K, S. Shalini, Vinoth Perumal, Sridharan Vellaisamy, Yuba Eiji, Harada Atsushi, Krishnan Uma Maheswari

    ACS BIOMATERIALS SCIENCE & ENGINEERING   5 ( 3 )   1215 - 1227   2019.03( ISSN:2373-9878

  • Highly Ordered Polypeptide with UCST Phase Separation Behavior.

    Kuroyanagi S, Shimada N, Fujii S, Furuta T, Harada A, Sakurai K, Maruyama A

    Journal of the American Chemical Society   141 ( 3 )   1261 - 1268   2019.01( ISSN:0002-7863

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  • Highly ordered polypeptide with UCST phase-separation behavior Reviewed

    S. Kuroyanagi, N. Shimada, S. Fujii, T. Furuta, A. Harada, K. Sakurai, A. Maruyama

    Journal of the American Chemical Society 雑誌   141 ( 3 )   1261 - 1268   2019.01

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    Kind of work:Joint Work  

  • Hyaluronic acid-based pH-sensitive polymer-modified liposomes for cell-specific intracellular drug delivery systems Reviewed

    M. Miyazaki, E. Yuba, H. Hayashi, A. Harada, K. Kono

    Bioconjugate Chem. 雑誌   29   2018.01

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    Kind of work:Joint Work  

  • Bleomycin-loaded pH-sensitive polymer-lipid-incorporated liposomes for cancer chemotherapy Reviewed

    E. Yuba, T. Osaki, M. Ono, S. Park, A. Harada, M. Yamashita, K. Azuma, T. Tsuka, N. Ito, T. Imagawa, Y. Okamoto

    Polymer 雑誌   10   2018.01

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    Kind of work:Joint Work  

  • TiO2ナノ粒子内包ポリイオンコンプレックスミセルによる疎水性プロドラッグの可溶化と超音波照射効果 Reviewed

    山本聡, 古川和樹, 小野雅文, 弓場英司, 原田敦史, 河野健司

    高分子論文集 雑誌   75   42 - 47   2018.01

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    Kind of work:Joint Work  

  • In vitro sonodynamic therapeutic effect of polyion complex micelles incorporating titanium dioxide nanoparticles Reviewed

    S. Yamamoto, M. Ono, E. Yuba, A. Harada

    Nanomaterials 雑誌   7   2017.09

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    Kind of work:Joint Work  

  • 93. Ultrasound-dependent cytoplasmic internalization of a peptide-sonosensitizer conjugate Reviewed

    Y. Inaba, K. Watanabe, M. Kitamatsu, E. Nakata, A. Harada, T. Ohtsuki

    Bioorganic & Medicinal Chemistry 雑誌   25   4212 - 4217   2017.08

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    Kind of work:Joint Work  

  • Development of pH-sensitive dextran derivatives with strong adjuvant function and their application to antigen delivery Reviewed

    E. Yuba, S. Uesugi, M. Miyazaki, Y. Kado, A. Harada, K. Kono

    Membranes 雑誌   7   2017.08

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    Kind of work:Joint Work  

  • pH-sensitive polymer-modified liposome-based immunity-inducing system: effects of inclusion of cationic lipid and CpG-DNA Reviewed

    Y. Yoshizaki, E. Yuba, N. Sakaguchi, K. Koiwai, A. Harada, K. Kono

    Biomaterials 雑誌 Elsevier B.V.   141   272 - 283   2017.07

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    Kind of work:Joint Work  

  • Effect of the side chain spacer structure on the pH-responsive properties of polycarboxylates Reviewed

    A. Harada, R. Teranishi, E. Yuba, K. Kono

    Journal of Biomaterials Science: Polymer Edition, 雑誌 Taylor & Francis online   28   1028 - 1035   2017.05

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    Kind of work:Joint Work  

  • Potentiation of cancer immunity-inducing effect by pH-sensitive polysaccharide-modified liposomes with combination of TGF-β type I receptor inhibitor-embedded liposomes Reviewed

    E. Yuba, S. Uesugi, Y. Yoshizaki, A. Harada, K. Kono

    Med. Res. Arch. 雑誌 KEI Journals   5 ( 5 )   2017.05

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    Kind of work:Joint Work  

  • Preparation of dual-stimuli-responsive liposomes using methacrylate-based copolymers with pH and temperature sensitivities for precisely controlled release Reviewed

    T.Sugimoto, N. Yamazaki, T. Hayashi, E. Yuba, A. Harada, A. Kotaka, C. Shinde, T. Kumei, Y. Sumida, M. Fukushima, Y. Munekata, K. Maruyama, K. Kono

    Colloids and Surfaces B: Biointerfaces 雑誌 Elsevier B.V.   155   449 - 458   2017.04

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    Kind of work:Joint Work  

  • Bioactive polysaccharide-based pH-sensitive polymers for cytoplasmic delivery of antigen and activation of antigen-specific immunity Reviewed

    E. Yuba, A. Yamaguchi, Y. Yoshizaki, A. Harada, K. Kono

    Biomaterials 雑誌 Elsevier B.V.   120   32 - 45   2016.12

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    Kind of work:Joint Work  

  • Doxorubicin delivery using pH and redox dual-responsive hollow nanocapsules with a cationic electrostatic barrier Reviewed

    R. Teranishi, R. Matsuki, E. Yuba, A. Harada, K. Kono

    Pharmaceutics 雑誌 MDPI   9 ( 1 )   2016.12

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    Kind of work:Joint Work  

  • Improvement of peptide-based tumor immunotherapy using pH-sensitive fusogenic polymer-modified liposomes Reviewed

    Y. Yoshizaki, E. Yuba, T. Komatsu, K. Udaka, A. Harada, K. Kono

    Molecules 雑誌 MDPI   21 ( 10 )   2016.09

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    Kind of work:Joint Work  

  • An oncofetal antigen, IMP-3-derived long peptides induce immune responses of both helper T cells and CTLs Reviewed

    M. Hirayama, Y. Tomita, A. Yuno, H. Tsukamoto, S. Senju, Y. Imamura, M. A. Sayem, A. Irie, Y. Yoshitake, D. Fukuma, M. Shinohara, A. Hamada, H. Jono, E. Yuba, K. Kono, K. Yoshida, T. Tsunoda, H. Nakayama, Y. Nishimura

    OncoImmunology 雑誌 Taylor & Francis online   5   2016.01

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    Kind of work:Joint Work  

  • Multifunctional liposomes having target specificity, temperature-triggered release, and near-infrared fluorescence imaging for tumor-specific chemotherapy Reviewed

    K. Kono, M. Takashima, E. Yuba, A. Harada, Y. Hiramatsu, H. Kitagawa, T. Otani, K. Maruyama, S. Aoshima

    J. Control. Release 雑誌 Elsevier B.V.   216   67 - 77   2015.10

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    Kind of work:Joint Work  

  • pH-Sensitive polymer-liposome-based antigen delivery systems potentiated with interferon-γ gene lipoplex for efficient cancer immunotherapy Reviewed

    E. Yuba, Y. Kanda, Y. Yoshizaki, R. Teranishi, A. Harada, K. Sugiura, T. Izawa, J. Yamate, N. Sakaguchi, K. Koiwai, K. Kono

    Biomaterials 雑誌 Elsevier B.V.   67   214 - 224   2015.10

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    Kind of work:Joint Work  

  • Gene expression of ternary complexes through the compaction of nanofiber-polyplexes by mixing with Lipofectamine Reviewed

    R. Aono, K. Nomura, E. Yuba, A. Harada, K. Kono

    Biomaterials Science 雑誌 RSC publishing   3   764 - 770   2015.05

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    Kind of work:Joint Work  

  • PAMAM dendron lipid assemblies that undergo structural transition in response to weakly acidic pH and their cytoplasmic delivery capability Reviewed

    T. Doura, M. Yamada, R. Teranishi, Y. Yamamoto, T. Sugimoto, E. Yuba, A. Harada, K. Kono

    Langmuir 雑誌 ACS publication   31 ( 18 )   5105 - 5114   2015.05

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    Kind of work:Joint Work  

  • Facile construction of well-defined fullerene-dendrimer supramolecular nanocomposites for bioapplications Reviewed

    X. Li, Y. Watanabe, E. Yuba, A. Harada, T. Haino, K. Kono

    Chem. Commun. 雑誌 RSC publishing   51   2851 - 2854   2015.01

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    Kind of work:Joint Work  

  • Dually functionalized dendrimers by temperature-sensitive surface modification and gold nanoparticle loading for biomedical application Reviewed

    K. Kono, K. Takeda, X. Li, E. Yuba, A. Harada, T. Ozaki, S. Mori

    RSC Adv. 雑誌 RSC publishing   4   27811 - 27819   2014.06

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    Kind of work:Joint Work  

  • Synthesis of oligoethylene glycol-modified hyperbranched polyglycidols for dual sensitization of liposomes to pH and temperature Reviewed

    K. Kono, T. Kaiden, E. Yuba, Y. Sakanishi, A. Harada

    J. Taiwan Institute of Chemical Engineers 雑誌 Elsevier B.V.   45   3054 - 3061   2014.06

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    Kind of work:Joint Work  

  • Cationic polymers with inhibition ability of DNA condensation elevate gene expression Reviewed

    A. Harada, Y. Kimura, K. Kono

    ChemBioChem 雑誌   11 ( 14 )   1985 - 1988   2010.09

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    Kind of work:Joint Work  

  • Effective tolerance to serum proteins of head-tail type polycation vectors by PEGylation at the periphery of the head block Reviewed

    A. Harada, Y. Kimura, C. Kojima, K. Kono

    Biomacromolecules 雑誌   11 ( 4 )   1036 - 1042   2010.04

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    Kind of work:Joint Work  

  • Spontaneous formation of narrowly-distributed self-assembly from polyamidoamine dendron-poly(L-lysine) block copolymers through helix-coil transition of poly(L-lysine) block Reviewed

    A. Harada. K. Nakanishi, S. Ichimura, C. Kojima, K. Kono

    Journal of Polymer Science Part A: Polymer Chemistry 雑誌   47 ( 4 )   1217 - 1223   2009.02

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    Kind of work:Joint Work  

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Books and Other Publications

  • 金属ナノ粒子の合成・設計・制御と応用技術 : 種々の合成法、サイズ・形状の制御と評価、分散・配合技術、応用展開

    米澤 徹、有村 英俊、中村 貴宏、中川 政俊、安田 啓司、熊木 大介、横山 俊、時任 静士、宮川 雅矢、武田 真一、田中 秀樹、永田 達己、浜本 真央、大洞 康嗣、柳生 裕聖、 棚橋 一郎、小川 智之、?橋 幸奈、清長 友和、新森 英之、森 康貴、須川 晃資、 蟹江 澄志、金子 真大、塩見 昌平、井藤 彰、徳永 智春、孫 術益、北山 雄己哉、板垣 賢広、原田 敦史、椎木 弘、バラチャンドラン ジャヤデワン、松井 響平、森 崇充、田邉 壮、和田 仁、山本 陽二郎( Role: Joint author)

    サイエンス&テクノロジー  2022.12  ( ISBN:9784864282482

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    Total pages:533 p, 図版 [25] p  

MISC

  • 【バイオマテリアル解析の基礎】高分子ナノ組織体の物理化学的特性解析

    原田 敦史

    バイオマテリアル-生体材料-   40 ( 1 )   44 - 47   2022.01( ISSN:1347-7080

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    ドラッグデリバリーシステムとしての利用を目的として、高分子鎖が自己組織化することによって形成される高分子ナノ組織体(高分子ミセルやポリマーソーム)の開発されている。有用なDDS開発においては、in vitroやin vivoでの機能評価だけでなく高分子ナノ組織体の物理化学的特性解析が重要である。本稿では、形態観察、動的光散乱測定による粒径評価、蛍光プローブを利用した特性解析について概説する。(著者抄録)

Presentations

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Grant-in-Aid for Scientific Research

  • 微粒子界面光反応を基盤とした生分解性カプセル創製技術の確立と機能性微粒子創出

    Grant-in-Aid for Scientific Research(B)  2022

  • 高感度・高精度癌診断を実現する蛍光Turn-on型人工抗体ナノセンサの開発

    Grant-in-Aid for Challenging Research (Pioneering)/(Exploratory)  2022

  • 低侵襲癌治療のための音増感剤デリバリーナノコンポジットの開発

    Grant-in-Aid for Scientific Research(B)  2022

  • 低侵襲癌治療のための音増感剤デリバリーナノコンポジットの開発

    Grant-in-Aid for Scientific Research(B)  2021

  • 微粒子界面光反応を基盤とした生分解性カプセル創製技術の確立と機能性微粒子創出

    Grant-in-Aid for Scientific Research(B)  2021

Charge of on-campus class subject

  • 高分子化学II

    2022   Weekly class   Undergraduate

  • 応用化学実験I

    2022   Weekly class   Undergraduate

  • 応用化学概論

    2022   Weekly class   Undergraduate

  • 応用化学実験III

    2022   Weekly class   Undergraduate

  • 基礎化学実験

    2022   Weekly class   Undergraduate

  • 物質化学生命系特別演習

    2022   Intensive lecture   Graduate school

  • 物質化学生命系特別演習第1

    2022   Intensive lecture   Graduate school

  • Advanced Applied Chemistry III

    2021    

  • Polymer Materials Chemistry

    2021    

  • Biopolymer

    2021    

  • Polymer Chemistry II

    2021    

  • Laboratory Chemistry

    2021   Practical Training  

  • Advanced Biopolymer Chemistry

    2021    

  • Selected Topics in Biopolymer Chemistry

    2021    

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Faculty development activities

  • 大阪府立大手前高等学校サイエンス探求最終報告会 指導助言  2022

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    大阪府立大手前高等学校SSH事業における課題研究「サイエンス探究」の研究成果(3年生SSコース)を最終報告会に出席し、指導助言を行った。

  • 大阪府立大手前高等学校サイエンス探求中間報告会 指導助言  2022

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    大阪府立大手前高等学校のSSH事業における課題研究「サイエンス探究」の研究成果(2年生SSコース)の中間報告会に出席し、指導助言を行った。

Number of instructed thesis, researches

  • 2022

    Number of instructed the graduation thesis:

    [Number of instructed the Master's Program] (previous term):[Number of instructed the Master's Program] (letter term):2

    [Number of master's thesis reviews] (chief):[Number of master's thesis reviews] (vice-chief):10

    [Number of doctoral thesis reviews] (chief):[Number of doctoral thesis reviews] (vice-chief):0

Visiting Lectures ⇒ Link to the list of Visiting Lectures

  • 医療に貢献する高分子材料

    Category:Science (mathematics, physics, chemistry, biology, geology, biochemistry), Engineering (machinery, electronics / physics, electrical / electronics, electrical information, chemical biotechnology, architecture, cities (civil engineering / environment), material chemistry, aerospace, marine systems, applied chemistry, chemistry, materials)

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    Audience:Schoolchildren, Junior high school students, High school students, College students, Teachers, Researchers, General

    Keyword:高分子・バイオマテリアル 

    コンタクトレンズやiPS細胞を培養する器材などに、さまざまな高分子材料が利用されており、高分子材料は医療には欠かせないものになっている。医療応用されている高分子材料の性質と用途について概説する。