Updated on 2024/04/17

写真a

 
TSUMURAYA Takeshi
 
Organization
Graduate School of Science Department of Biological Chemistry Professor
School of Science Department of Biological Chemistry
Title
Professor
Affiliation
Institute of Science
Affiliation campus
Nakamozu Campus

Position

  • Graduate School of Science Department of Biological Chemistry 

    Professor  2022.04 - Now

  • School of Science Department of Biological Chemistry 

    Professor  2022.04 - Now

Degree

  • 理学博士 （ University of Tsukuba ）

Research Areas

  • Nanotechnology/Materials / Chemical biology

  • Nanotechnology/Materials / Bio chemistry

  • Nanotechnology/Materials / Chemistry and chemical methodology of biomolecules

  • Nanotechnology/Materials / Chemistry and chemical methodology of biomolecules  / 天然物化学

  • Nanotechnology/Materials / Bio chemistry  / タンパク質・酵素化学

  • Nanotechnology/Materials / Chemical biology  / 化学プローブ

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Research Interests

  • transition-state analog

  • directed evolution

  • biofunctional moletule

  • library

  • monoclonal antibody

  • hybridoma

Professional Memberships

  • 酵素工学研究会

    2006.10 - Now   Domestic

  • Protein Society

  • AOAC (The Association of Analytical Communities) International

  • コンビナトリアル・バイオエンジニアリング研究会

  • Japanese Society of Enzyme Engineering

  • KINKI CHEMICAL SOCIETY, JAPAN

  • American Chemical Society

  • THE SOCIETY OF SYNTHETIC ORGANIC CHEMISTRY, JAPAN

  • PROTEIN SCIENCE SOCIETY OF JAPAN

  • THE PHARMACEUTICAL SOCIETY OF JAPAN

  • THE CRYSTALLOGRAPHIC SOCIETY OF JAPAN

  • The Society for Biotechnology, Japan

  • 日本化学会 フロンティア生命化学研究会

  • THE CHEMICAL SOCIETY OF JAPAN

  • The Japanese Peptide Society

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Job Career (off-campus)

  • Osaka Metropolitan University   Department of Biological Chemistry, Graduate School of Science

    2022.04 - Now

  • Osaka Prefecture University   Graduate School of Science, Department of Biological Science

    2017.04 - Now

Papers

  • Sensitive Detection of Ciguatoxins Using a Neuroblastoma Cell-Based Assay with Voltage-Gated Potassium Channel Inhibitors

    Toshiaki Yokozeki, Madoka Kawabata, Kazuhiro Fujita, Masahiro Hirama, Takeshi Tsumuraya

    Toxins   16 ( 3 )   118 - 118   2024.02（ eISSN:2072-6651 ）

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    Publishing type：Research paper (scientific journal)  

    Ciguatoxins (CTXs) are neurotoxins responsible for ciguatera poisoning (CP), which affects more than 50,000 people worldwide annually. The development of analytical methods to prevent CP is a pressing global issue, and the N2a assay is one of the most promising methods for detecting CTXs. CTXs are highly toxic, and an action level of 0.01 μg CTX1B equivalent (eq)/kg in fish has been proposed. It is desirable to further increase the detection sensitivity of CTXs in the N2a assay to detect such low concentrations reliably. The opening of voltage-gated sodium channels (NaV channels) and blocking of voltage-gated potassium channels (KV channels) are thought to be involved in the toxicity of CTXs. Therefore, in this study, we developed an assay that could detect CTXs with higher sensitivity than conventional N2a assays, using KV channel inhibitors as sensitizing reagents for N2a cells. The addition of the KV channel inhibitors 4-aminopyridine and tetraethylammonium chloride to N2a cells, in addition to the traditional sensitizing reagents ouabain and veratridine, increased the sensitivity of N2a cells to CTXs by up to approximately 4-fold. This is also the first study to demonstrate the influence of KV channels on the toxicity of CTXs in a cell-based assay.

    DOI： 10.3390/toxins16030118

    PubMed

  • Evaluation of relative potency of calibrated ciguatoxin congeners by near-infrared fluorescent receptor binding and neuroblastoma cell-based assays

    Toshiaki Yokozeki, Yuka Hama, Kazuhiro Fujita, Tomoji Igarashi, Masahiro Hirama, Takeshi Tsumuraya

    Toxicon   230   107161 - 107161   2023.07（ ISSN:0041-0101 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.toxicon.2023.107161

    PubMed

  • Cell immunolocalization of ciguatoxin-like compounds in the benthic dinoflagellate Gambierdiscus australes M. Chinain & M.A. Faust by confocal microscopy International coauthorship

    120   102353   2022.12（ ISSN:15689883 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    DOI： 10.1016/j.hal.2022.102353

    PubMed

  • A smartphone-controlled amperometric immunosensor for the detection of Pacific ciguatoxins in fish Reviewed International coauthorship

    374   2022.04

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    Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    DOI： 10.1016/j.foodchem.2021.131687. Epub 2021 Dec 2.

  • Electrochemical biosensor for the dual detection of Gambierdiscus australes and Gambierdiscus excentricus in field samples. First report of G. excentricus in the Balearic Islands. Reviewed

    Greta Gaiani, Francesca Cucchi, Anna Toldrà, Karl B Andree, María Rey, Takeshi Tsumuraya, Ciara K O'Sullivan, Jorge Diogène, Mònica Campàs

    The Science of the total environment   150915 - 150915   2021.10

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Several genera of marine dinoflagellates are known to produce bioactive compounds that affect human health. Among them, Gambierdiscus and Fukuyoa stand out for their ability to produce several toxins, including the potent neurotoxic ciguatoxins (CTXs), which accumulate through the food web. Once fishes contaminated with CTXs are ingested by humans, it can result in an intoxication named ciguatera. Within the two genera, only some species are able to produce toxins, and G. australes and G. excentricus have been highlighted to be the most abundant and toxic. Although the genera Gambierdiscus and Fukuyoa are endemic to tropical areas, their presence in subtropical and temperate regions has been recently recorded. In this work, the combined use of species-specific PCR primers for G. australes and G. excentricus modified with short oligonucleotide tails allowed the development of a multiplex detection system for these two toxin-producing species. Simultaneous detection was achieved using capture probes specific for G. australes and G. excentricus immobilized on maleimide-coated magnetic beads (MBs), separately placed on the working electrodes of a dual electrode array. Additionally, a rapid DNA extraction technique based on a portable bead beater system and MBs was developed, significantly reducing the extraction time (from several hours to 30 min). The developed technique was able to detect as low as 10 cells of both Gambierdiscus species and allowed the first detection of G. excentricus in the Balearic Islands in 8 out of the 12 samples analyzed. Finally, field samples were screened for CTXs with an immunosensor, successfully reporting 13.35 ± 0.5 pg CTX1B equiv. cell-1 in one sample and traces of toxins in 3 out of the 9 samples analyzed. These developments provide rapid and cost-effective strategies for ciguatera risk assessment, with the aim of guaranteeing seafood safety.

    DOI： 10.1016/j.scitotenv.2021.150915

    PubMed

  • Chemical Modification of Phage-Displayed Helix-Loop-Helix Peptides to Construct Kinase-Focused Libraries. Reviewed

    Daisuke Fujiwara, Kousuke Mihara, Ryo Takayama, Yusuke Nakamura, Mitsuhiro Ueda, Takeshi Tsumuraya, Ikuo Fujii

    Chembiochem : a European journal of chemical biology   2021.10

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Conformationally constrained peptides hold promise as molecular tools in chemical biology and as a new modality in drug discovery. The construction and screening of a target-focused library could be a promising approach for the generation of de novo ligands or inhibitors against target proteins. Here, we have prepared a protein kinase-focused library by chemically modifying helix-loop-helix (HLH) peptides displayed on phage and subsequently tethered to adenosine. The library was screened against aurora kinase A (AurA). The selected HLH peptide Bip-3 retained the α-helical structure and bound to AurA with a KD value of 13.7 μM. Bip-3 and the adenosine-tethered peptide Bip-3-Adc provided IC50 values of 103 μM and 7.7 μM, respectively, suggesting that Bip-3-Adc bivalently inhibited AurA. In addition, the selectivity of Bip-3-Adc to several protein kinases was tested, and was highest against AurA. These results demonstrate that chemical modification can enable the construction of a kinase-focused library of phage-displayed HLH peptides.

    DOI： 10.1002/cbic.202100450

    PubMed

  • Electrochemical biosensor for the dual detection of Gambierdiscus australes and Gambierdiscus excentricus in field samples. First report of Gambierdiscus excentricus in the Balearic Islands Reviewed

    Greta Gaiani, Francesca Cucchi, Anna Toldrà, Karl B. Andree, María Rey, Takeshi Tsumuraya, Ciara K. O'Sullivan, Jorge Diogène, Mònica Campàs

    Science of the Total Environment 雑誌 Elsevier   806 ( 4 )   2021.10

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    Kind of work：Joint Work  

  • Rapid detection of ciguatoxins in Gambierdiscus and Fukuyoa with immunosensing tools G. Reviewed

    G. Gaiani, S. Leonardo, A. Tudo, A. Toldra, M. Rey, K. B. Andree, T. Tsumuraya, M. Hirama, J. Diogene, C. K. O'Sullivan, C. Alcaraz, M. Campas

    Ecotoxicology and Environmental Safety 雑誌 ELSEVIER   204   2020.11

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    Kind of work：Joint Work  

  • Rapid detection of ciguatoxins in Gambierdiscus and Fukuyoa with immunosensing tools Reviewed

    G. Gaiani, S. Leonardo, Tudó, A. Toldrà, M. Rey, K. B. Andree, T. Tsumuraya, M. Hirama, J. Diogène, C. K. O'Sullivan, C. Alcaraz, M. Campàs

    Ecotoxicology and Environmental Safety   204   2020.11（ ISSN:0147-6513 ） （ eISSN:1090-2414 ）

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    Publishing type：Research paper (scientific journal)  

    Consumption of seafood contaminated with ciguatoxins (CTXs) leads to a foodborne disease known as ciguatera. Primary producers of CTXs are epibenthic dinoflagellates of the genera Gambierdiscus and Fukuyoa. In this study, thirteen Gambierdiscus and Fukuyoa strains were cultured, harvested at exponential phase, and CTXs were extracted with an implemented rapid protocol. Microalgal extracts were obtained from pellets with a low cell abundance (20,000 cell/mL) and were then analyzed with magnetic bead (MB)-based immunosensing tools (colorimetric immunoassay and electrochemical immunosensor). It is the first time that these approaches are used to screen Gambierdiscus and Fukuyoa strains, providing not only a global indication of the presence of CTXs, but also the ability to discriminate between two series of congeners (CTX1B and CTX3C). Analysis of the microalgal extracts revealed the presence of CTXs in 11 out of 13 strains and provided new information about Gambierdiscus and Fukuyoa toxin profiles. The use of immunosensing tools in the analysis of microalgal extracts facilitates the elucidation of further knowledge regarding these dinoflagellate genera and can contribute to improved ciguatera risk assessment and management.

    DOI： 10.1016/j.ecoenv.2020.111004

    PubMed

  • Further advance of Gambierdiscus Species in the Canary Islands, with the First Report of Gambierdiscus belizeanus. Reviewed

    Àngels Tudó, Greta Gaiani, Maria Rey Varela, Takeshi Tsumuraya, Karl B Andree, Margarita Fernández-Tejedor, Mònica Campàs, Jorge Diogène

    Toxins   12 ( 11 )   2020.10

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Ciguatera Poisoning (CP) is a human food-borne poisoning that has been known since ancient times to be found mainly in tropical and subtropical areas, which occurs when fish or very rarely invertebrates contaminated with ciguatoxins (CTXs) are consumed. The genus of marine benthic dinoflagellates Gambierdiscus produces CTX precursors. The presence of Gambierdiscus species in a region is one indicator of CP risk. The Canary Islands (North Eastern Atlantic Ocean) is an area where CP cases have been reported since 2004. In the present study, samplings for Gambierdiscus cells were conducted in this area during 2016 and 2017. Gambierdiscus cells were isolated and identified as G. australes, G. excentricus, G. caribaeus, and G. belizeanus by molecular analysis. In this study, G. belizeanus is reported for the first time in the Canary Islands. Gambierdiscus isolates were cultured, and the CTX-like toxicity of forty-one strains was evaluated with the neuroblastoma cell-based assay (neuro-2a CBA). G. excentricus exhibited the highest CTX-like toxicity (9.5-2566.7 fg CTX1B equiv. cell-1) followed by G. australes (1.7-452.6.2 fg CTX1B equiv. cell-1). By contrast, the toxicity of G. belizeanus was low (5.6 fg CTX1B equiv. cell-1), and G. caribaeus did not exhibit CTX-like toxicity. In addition, for the G. belizeanus strain, the production of CTXs was evaluated with a colorimetric immunoassay and an electrochemical immunosensor resulting in G. belizeanus producing two types of CTX congeners (CTX1B and CTX3C series congeners) and can contribute to CP in the Canary Islands.

    DOI： 10.3390/toxins12110692

    PubMed

  • Further advance of Gambierdiscus Species in the Canary Islands, with the First Report of Gambierdiscus belizeanus Reviewed

    Tudó À, Gaiani G, Varela MR, Tsumuraya T, Andree KB, Fernández-Tejedor M, Campàs M, Diogène J.

    Toxins 雑誌 MDPI, Basel   12   2020.10

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    Kind of work：Joint Work  

  • Addressing the Analytical Challenges for the Detection of Ciguatoxins Using an Electrochemical Biosensor. Reviewed

    Sandra Leonardo, Greta Gaiani, Takeshi Tsumuraya, Masahiro Hirama, Jean Turquet, Núria Sagristà, Maria Rambla-Alegre, Cintia Flores, Josep Caixach, Jorge Diogène, Ciara K O'Sullivan, Carles Alcaraz, Mònica Campàs

    Analytical chemistry   92 ( 7 )   4858 - 4865   2020.04

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    The importance of ciguatoxins (CTXs) in seafood safety and their emerging occurrence in locations far away from tropical areas highlight the need for simple and low-cost methods for the sensitive and rapid detection of these potent marine toxins to protect seafood consumers. Herein, an electrochemical immunosensor for the detection of CTXs is presented. A sandwich configuration is proposed, using magnetic beads (MBs) as immobilization supports for two capture antibodies, with their combination facilitating the detection of CTX1B, CTX3C, 54-deoxyCTX1B, and 51-hydroxyCTX3C. PolyHRP-streptavidin is used for the detection of the biotinylated detector antibody. Experimental conditions are first optimized using colorimetry, and these conditions are subsequently used for electrochemical detection on electrode arrays. Limits of detection at the pg/mL level are achieved for CTX1B and 51-hydroxyCTX3C. The applicability of the immunosensor to the analysis of fish samples is demonstrated, attaining detection of CTX1B at contents as low as 0.01 μg/kg and providing results in correlation with those obtained using mouse bioassay (MBA) and cell-based assay (CBA), and confirmed by liquid chromatography coupled to high-resolution mass spectrometry (LC-ESI-HRMS). This user-friendly bioanalytical tool for the rapid detection of CTXs can be used to mitigate ciguatera risk and contribute to the protection of consumer health.

    DOI： 10.1021/acs.analchem.9b04499

    PubMed

  • Addressing the Analytical Challenges for the Detection of Ciguatoxins Using an Electrochemical Biosensor Reviewed

    Leonardo S, Gaiani G, Tsumuraya T, Hirama M, Turquet J, Sagristà N, Rambla-Alegre M, Flores C, Caixach J, Diogène J, O'Sullivan CK, Alcaraz C, Campàs M.

    Analytical Chemistry 雑誌 American Chemical Society   92 ( 7 )   4858 - 4865   2020.03

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    Kind of work：Joint Work  

  • Rationally Designed Synthetic Haptens to Generate Anti-Ciguatoxin Monoclonal Antibodies, and Development of a Practical Sandwich ELISA to Detect Ciguatoxins. Reviewed

    Takeshi Tsumuraya, Masahiro Hirama

    Toxins   11 ( 9 )   2019.09

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    Authorship：Lead author   Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    "Ciguatera" fish poisoning (CFP) is one of the well-known food poisoning caused by the ingestion of fish that have accumulated trace amounts of ciguatoxins (CTXs). CFP affects more than 50,000 individuals annually. The difficulty in preventing CFP comes from the lack of reliable methods for analysis of CTXs in contaminated fish, together with the normal appearance, taste, and smell of CTX-contaminated fish. Thus, a sensitive, accurate, routine, and portable analytical method to detect CTXs is urgently required. Monoclonal antibodies (mAbs) specific against either wing of major CTX congeners (CTX1B, 54-deoxyCTX1B, CTX3C, and 51-hydroxyCTX3C) were generated by immunizing mice with rationally designed synthetic haptens-KLH conjugates instead of the CTXs. Haptenic groups with a surface area greater than 400 Å2 are required to produce mAbs that can strongly bind to CTXs. Furthermore, a highly sensitive fluorescence-based sandwich enzyme-linked immunosorbent assay (ELISA) was developed. This protocol can detect and quantify four major CTX congeners (CTX1B, 54-deoxyCTX1B, CTX3C, and 51-hydroxyCTX3C) with a limit of detection (LOD) of less than 1 pg/mL. The LOD determined for this sandwich ELISA is sufficient to detect CTX1B-contaminated fish at the FDA guidance level of 0.01 ppb.

    DOI： 10.3390/toxins11090533

    PubMed

  • Rationally Designed Synthetic Haptens to Generate Anti-Ciguatoxin Monoclonal Antibodies, and Development of a Practical Sandwich ELISA to Detect Ciguatoxins Reviewed

    Takeshi Tsumuraya and Masahiro Hirama

    Toxins 雑誌 MDPI   11   1 - 16   2019.09

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    Kind of work：Joint Work  

  • Highly Sensitive and Fluorescent Sandwich ELISA for Ciguatoxins Reviewed

    *Takeshi Tsumuraya, Takeshi Sato, Masahiro Hirama, and Ikuo Fujii

    Anal. Chem. 雑誌   90   7318 - 7324   2018

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    Kind of work：Joint Work  

  • Expanding the Scope of Functionalized Small Nonprotein Components for Holoabzyme 27C1 Reviewed

    Fumihiro Ishikawa, Masato Shirahashi, Hiroshi Hayakawa, Genzoh Tanabe, Takeshi Tsumuraya, Ikuo Fujii

    CHEMISTRY SELECT 雑誌   3   9313 - 9317   2018

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    Kind of work：Joint Work  

  • Structural basis of the broad substrate tolerance of the antibody 7B9-catalyzed hydrolysis of p-nitrobenzyl esters Reviewed

    Naoki Miyamoto, Miho Yoshimura, Yuji Okubo, Kayo Suzuki-Nagata, Takeshi Tsumuraya, Nobutoshi Ito, Ikuo Fujii

    Bioorg. Med. Chem., 雑誌   26   1412 - 1417   2018

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    Kind of work：Joint Work  

  • Effects of conformational strain of substrate on binding kinetics of catalytic antibody Reviewed

    *Masayuki Oda, Takeshi Tsumuraya, and Ikuo Fujii

    Biophysics and Physicobiology 雑誌   13   135 - 138   2016

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    Kind of work：Joint Work  

  • Abzyme engeering by site-directed chemical mutation Reviewed

    Fumihiro Ishikawa, Masato Shirahashi, Hiroshi Hayakawa, Asako Yamaguchi, Takatsugu Hirokawa, Takeshi Tsumuraya, and *Ikuo Fujii

    ACS Chemical Biology 雑誌   11   2803 - 2811   2016

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    Kind of work：Joint Work  

  • Preparation of Anti-ciguatoxin monoclonal antibodies using synthetic haptens. Sandwich ELISA detection of ciguatoxins Reviewed

    *Takeshi Tsumuraya, Ikuo Fujii, and Masahiro Hirama

    J. AOAC (The Association of Analytical Communities) Int. 雑誌   97   373 - 379   2014

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    Kind of work：Joint Work  

  • Antibody-catalyzed decarboxylation and aldol reactions using a primary amine molecule as a functionalized small nonprotein component Reviewed

    Fumihiro Ishikawa, Kouki Uno, Masato Nishikawa, Takeshi Tsumuraya, *Ikuo Fujii

    Bioorg. Med. Chem. 雑誌   21   7011 - 7017   2013

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    Kind of work：Joint Work  

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Books and Other Publications

  • 酵素の反応速度解析「バイオサイエンス実験：入門から応用へ」

    藤井　郁雄，円谷　健，藤原　大佑（ Role： Joint author）

    大阪公立大学共同出版会  2016.04 

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    Responsible for pages：89-107  

  • タンパク質の定量「バイオサイエンス実験：入門から応用へ」

    藤井　郁雄，円谷　健，藤原　大佑（ Role： Joint author）

    大阪公立大学共同出版会  2016.04 

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    Responsible for pages：25-32  

  • パパイン処理による抗体のFabの調製

    円谷健，藤井郁雄（ Role： Joint author）

    NTS  2012.08 

  • 抗体酵素ーテーラーメイド人工酵素の創出

    円谷健，藤井郁雄（ Role： Joint author）

    NTS  2012.08 

  • 新しいくすりづくり：ケミカルバイオロジー

    藤井郁雄，円谷健，藤原大佑，多田俊治，木下誉富，恩田真紀，切畑光統，谷森紳治，神川憲，乾隆（ Role： Joint author）

    NTS  2011.12 

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    Responsible for pages：257-273  

  • 進化分子工学による人工酵素の創出

    円谷健，藤井郁雄（ Role： Joint author）

    化学同人  2010.09 

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    Responsible for pages：102-108  

  • 抗体エンジニアリングの最前線

    円谷　健，藤井　郁雄（ Role： Joint author）

    シーエムシー出版  2004.02 

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    Responsible for pages：17-28  

  • 抗体エンジニアリングの最前線

    円谷 健, 藤井 郁雄（ Role： Joint author ,  第3章 抗体酵素：免疫システムを用いた新規生体触媒の創出）

    シーエムシー出版 

  • 社会との垣根を越える大学の挑戦

    藤井 郁雄, 円谷 健, 藤原 大佑, 多田 俊治, 木下 誉富, 恩田 真紀, 切畑 光統, 谷森 紳治, 神川 憲, 乾 隆（ Role： Joint author ,  第14章 新しいくすりづくり：ケミカルバイオロジー）

    NTS 

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Presentations

  • An approach to ciguatera research based on anti-ciguatoxin monoclonal antibodies Invited International conference

    Takeshi Tsumuraya

    International Symposium in Okinawa, 2023, on Ciguatera and Related Marine Biotoxins: Post symposium of the ICHA2023 Hiroshima  2023.11  Organizing committee of International Symposium in Okinawa, 2023, on Ciguatera and Related Marine Biotoxins

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    Presentation type：Oral presentation (invited, special)  

  • Evaluation of relative potency of calibrated ciguatoxin congeners by near-infrared fluorescent receptor binding and neuroblastoma cell-based assays International conference

    Toshiaki Yokozeki, Yuka Hama, Kazuhiro Fujita, Tomoji Igarashi, Masahiro Hirama, Takeshi Tsumuraya

    International Symposium in Okinawa, 2023, on Ciguatera and Related Marine Biotoxins: Post symposium of the ICHA2023 Hiroshima  2023.11  Organizing committee of International Symposium in Okinawa, 2023, on Ciguatera and Related Marine Biotoxins: Post symposium of the ICHA2023 Hiroshima

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    Presentation type：Poster presentation  

  • Evaluation of differences in acid stability of ciguatoxin congeners from the Pacific by LC-MS/MS International conference

    Toshiaki Yokozeki, Kazuhiro Fujita, Tomoji Igarashi, Masahiro Hirama, Takeshi Yasumoto, Takeshi Tsumuraya

    The 20th International Conference on Harmful Algae (ICHA2023)  2023.11  International Society for the Study of Harmful Algae

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    Presentation type：Poster presentation  

  • Development of purification method for ciguatoxins in fish using lipid hydrolysis and application to near-infrared fluorescent receptor binding assay International conference

    Toshiaki Yokozeki, Kazuhiro Fujita, Tomoji Igarashi, Takeshi Tsumuraya, Takeshi Yasumoto

    International Symposium in Okinawa, 2023, on Ciguatera and Related Marine Biotoxins: Post symposium of the ICHA2023 Hiroshima  2023.11  Organizing committee of International Symposium in Okinawa, 2023, on Ciguatera and Related Marine Biotoxins: Post symposium of the ICHA2023 Hiroshima

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    Presentation type：Poster presentation  

  • Evaluation of Relative Potency of Calibrated Ciguatoxin Congeners by Near-infrared Fluorescent Receptor Binding and Neuroblastoma Cell-based Assays Domestic conference

    2023.09 

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    Presentation type：Oral presentation (general)  

  • Rationally Designed Synthetic Haptens to Generate Anti-Ciguatoxin Monoclonal Antibodies, and Development of a Practical Sandwich ELISA to Detect Ciguatoxins Invited International conference

    Takeshi Tsumuraya

    AOAC INTERNATIONAL 137th Annual Meeting & Exposition  2023.08  AOAC Int.

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    Presentation type：Oral presentation (invited, special)  

    Venue：New Orleans  

  • Evaluation of relative potency of calibrated ciguatoxin congeners by near-infrared fluorescent receptor binding and neuroblastoma cell-based assays International conference

    Toshiaki Yokozeki, Yuka Hama, Kazuhiro Fujita, Tomoji Igarashi, Masahiro Hirama, Takeshi Tsumuraya

    2023.08  AOAC International

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    Presentation type：Poster presentation  

  • 近赤外蛍光レセプターバインディングアッセイおよびN2aアッセイによる　　　　　　　　　　　　　　　　　シガトキシン同族体の相対効力評価 Domestic conference

    横関俊昭、濱悠佳、藤田和弘、五十嵐友二、平間正博、円谷　健

    2023 AOAC Japan Section  2023.07  AOAC Japan Section

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    Presentation type：Poster presentation  

    Venue：東京大学  

  • Smart biosensors for the detection of ciguatoxins International coauthorship International conference

    2022.10 

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    Presentation type：Poster presentation  

  • Cell immunolocalization of ciguatoxins in the benthic dinoflagellate Gambierdiscus australes International coauthorship International conference

    2022.10 

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    Presentation type：Poster presentation  

  • Development of novel fluorescent-labeled brevetoxins for receptor binding assay International conference

    Toshiaki Yokozeki, Takeshi Tsumuraya, Kazuhiro Fujita, Tomoji Igarashi, Takeshi Yasumoto

    PACIFICHEM 2021  2021.12 

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    Presentation type：Poster presentation  

  • Detection of Ciguatoxins based on anti-Ciguatoxin Monoclonal Antibodies Invited International conference

    T. Tsumuraya

    PACIFICHEM 2021  2021.12 

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    Presentation type：Poster presentation  

  • 有機合成化学との連携による海洋毒認識モノクローナル抗体の作製と微量検出法の開発 Invited Domestic conference

    円谷　健

    バイオインタラクション研究会代12回ワークショップ  2021.03  バイオインタラクション研究会

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    Presentation type：Oral presentation (invited, special)  

  • Smartpone-based electrochemical immunosensor for ciguatoxin detection International conference

    Sandra Keonardo, Takeshi Tsumuraya, Oshiro Naomasa, Masahiro Hirama, Jorge Digene, Monica Campas

    1st International Electronic Conference on Toxins  2021.01 

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    Presentation type：Poster presentation  

  • Detection of ciguatoxins in fish and algal samples with an electrochemical biosensor International conference

    Greta Gaiani, Sandra Leonardo, Takeshi Tsumuraya, Maria Rambla, Jorge Digene, Ciara Kathleen O'Sullivan, Carles Alcaraz, Monica Campas

    1st International Electronic Conference on Toxins  2021.01 

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    Presentation type：Poster presentation  

  • レセプターバインディングアッセイによるシガトキシン検出のための新規蛍光ブレベトキシンの作製 Domestic conference

    横関俊昭、円谷健、藤田和弘、五十嵐友二、安元健

    日本食品衛生学会代116回学術講演会  2020.11  日本食品衛生学会

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    Presentation type：Poster presentation  

  • レセプターバインディングアッセイによるシガトキシン検出のための新規蛍光ブレベトキシンの作製

    横関 俊昭, 円谷 健, 藤田 和弘, 五十嵐 友二, 安元 健

    日本食品衛生学会学術講演会講演要旨集  2020.11  (公社)日本食品衛生学会

  • Development of a Practical Sandwich ELISA to Detect Ciguatoxins Invited International conference

    Takeshi Tsumuraya

    EUROCIGUA INTERNATIONAL WORKSHOP 2020  2020.10 

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    Presentation type：Oral presentation (invited, special)  

  • ELISAによるシガトキシンの検出 Domestic conference

    伊藤史織、国吉杏子、大野祐美、岩屋あまね、佐久川さつき、小島尚、円谷健、平間正博、安元健、大城直雅、朝倉宏

    食品衛生学会代115回学術講演会  2019.11  食品衛生学会

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    Presentation type：Poster presentation  

  • ELISAによるシガトキシンの検出

    伊藤 史織, 國吉 杏子, 大野 祐美, 岩屋 あまね, 佐久川 さつき, 小島 尚, 円谷 健, 平間 正博, 安元 健, 大城 直雅, 朝倉 宏

    日本食品衛生学会学術講演会講演要旨集  2019.09  (公社)日本食品衛生学会

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Grant-in-Aid for Scientific Research

  • Development of Caribbean ciguatoxin-specific antibodies for prevention and treatment of ciguatera

    Grant-in-Aid for Scientific Research(C)  2024

Charge of on-campus class subject

  • 生物化学実験2

    2024   Weekly class   Undergraduate

  • 基礎有機化学

    2024   Weekly class   Undergraduate

  • ケミカルバイオロジー特論

    2024   Weekly class   Graduate school

  • 生物化学特別研究2A

    2024   Intensive lecture   Graduate school

  • 生物化学特別研究1A

    2024   Intensive lecture   Graduate school

  • 生物化学特別演習2A

    2024   Intensive lecture   Graduate school

  • 生物化学特別演習1A

    2024   Intensive lecture   Graduate school

  • 生物化学特別研究5A

    2024   Intensive lecture   Graduate school

  • 生物化学特別研究4A

    2024   Intensive lecture   Graduate school

  • 生物化学特別研究3A

    2024   Intensive lecture   Graduate school

  • 生物化学特別演習5A

    2024   Intensive lecture   Graduate school

  • 生物化学特別演習4A

    2024   Intensive lecture   Graduate school

  • 生物化学特別演習3A

    2024   Intensive lecture   Graduate school

  • 生物化学への招待

    2024   Weekly class   Graduate school

  • 基礎有機化学

    2024   Intensive lecture   Undergraduate

  • 研究企画ゼミナール1

    2023   Intensive lecture   Graduate school

  • 生物化学実験1

    2023   Weekly class   Undergraduate

  • 生物有機化学

    2023   Weekly class   Undergraduate

  • 生物化学概論

    2023   Weekly class   Undergraduate

  • 基礎有機化学B

    2023   Weekly class   Graduate school

  • 生物化学特別研究4B

    2023   Intensive lecture   Graduate school

  • 生物化学特別研究2B

    2023   Intensive lecture   Graduate school

  • 生物化学特別研究1B

    2023   Intensive lecture   Graduate school

  • 生物化学特別演習2B

    2023   Intensive lecture   Graduate school

  • 生物化学特別演習1B

    2023   Intensive lecture   Graduate school

  • 生物化学への招待

    2022   Weekly class   Undergraduate

  • 生物化学特別研究3A

    2022   Intensive lecture   Graduate school

  • 生物化学特別演習3A

    2022   Intensive lecture   Graduate school

  • ケミカルバイオロジー特論

    2022   Weekly class   Graduate school

  • 生物化学特別研究1A

    2022   Intensive lecture   Graduate school

  • 生物化学特別演習1A

    2022   Intensive lecture   Graduate school

  • 海外特別研究1

    2022   Intensive lecture   Graduate school

  • 生物化学特別研究1B

    2022   Intensive lecture   Graduate school

  • 生物化学特別演習1B

    2022   Intensive lecture   Graduate school

  • 研究企画ゼミナール1

    2022   Intensive lecture   Graduate school

  • 基礎有機化学

    2022   Weekly class   Undergraduate

  • 生物科学実験I

    2022   Weekly class   Undergraduate

  • 生物化学概論

    2022   Weekly class   Undergraduate

  • 海外特別研究2

    2022   Intensive lecture   Graduate school

  • 生物化学特別研究3B

    2022   Intensive lecture   Graduate school

  • 生物化学特別演習3B

    2022   Intensive lecture   Graduate school

  • 研究企画ゼミナール2

    2022   Intensive lecture   Graduate school

  • 基礎有機化学B/必:理<生化>選:<数物生地>市:時間割参照S

    2022   Weekly class   Undergraduate

  • 化学II２組生命環境科学域（理学）

    2022   Weekly class   Undergraduate

  • Fundamental Organic Chemistry

    2021    

  • Introduction to Life, Environment, and Advanced Sciences

    2021    

  • Experiment in Biological Science II

    2021   Practical Training  

  • Principles of Organic Chemistry

    2021    

  • Chemical Biology

    2021    

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Charge of off-campus class subject

  • 科学英語

    Institution：Osaka Prefecture University

  • 社会に生きる科学

    Institution：Osaka Prefecture University

  • 生物科学課題実験

    Institution：Osaka Prefecture University

  • 生物科学演習II

    Institution：Osaka Prefecture University

  • 生物科学演習I

    Institution：Osaka Prefecture University

  • 生物科学実験II

    Institution：Osaka Prefecture University

  • 生物科学実験I

    Institution：Osaka Prefecture University

  • 生物科学卒業研究

    Institution：Osaka Prefecture University

  • 生物学実験

    Institution：Osaka Prefecture University

  • 生命化学特論

  • 生化学IV

    Institution：Osaka Prefecture University

  • 生体分子構造特論

  • 機器分析学

    Institution：Osaka Prefecture University

  • 教職総合ゼミナール

    Institution：Osaka Prefecture University

  • 基礎有機化学

    Institution：Osaka Prefecture University

  • 化学II

    Institution：Osaka Prefecture University

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Media Coverage

  • 朝日新聞 Newspaper, magazine

    2019.04

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    シガテラ食中毒およびシガテラ食中毒原因毒素の検出に関して，朝日新聞 アピタルで専門家としてコメントした．(4/16, 17, 18, 19に掲載）

Visiting Lectures　⇒ Link to the list of Visiting Lectures

  • どのようにして食べてはいけない魚を見分けるのか？

    Category：Science (mathematics, physics, chemistry, biology, geology, biochemistry),　Agricultural science (applied biology, biofunctional chemistry, green space environmental science)

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    Audience：Junior high school students,　High school students,　College students,　Teachers,　Researchers,　General,　Company,　Civic organization

    Keyword：シガテラ　微量検出　バイオテクノロジー　モノクローナル抗体 

    バイオテクノロジーを用いたシガテラ毒の微量検出法の開発について講義する．