Updated on 2023/04/03

写真a

 
Hisamoto Hideaki
 
Organization
Graduate School of Engineering Division of Science and Engineering for Materials, Chemistry and Biology Professor
School of Engineering Department of Applied Chemistry
Title
Professor
Affiliation
Institute of Engineering
Contact information
メールアドレス
Affiliation campus
Nakamozu Campus

Position

  • Graduate School of Engineering Division of Science and Engineering for Materials, Chemistry and Biology 

    Professor  2022.04 - Now

  • School of Engineering Department of Applied Chemistry 

    Professor  2022.04 - Now

Degree

  • 博士(工学) ( Others )

Research Areas

  • Nanotechnology/Materials / Nano/micro-systems  / Micro/Nano devices

  • Nanotechnology/Materials / Analytical chemistry  / Analytical Chemistry

  • Life Science / Biomedical engineering  / Microanalytical Devices

  • Nanotechnology/Materials / Nano/micro-systems  / Microreactors

Research Interests

  • Functional dyes

  • Chemical sensors

  • Molecular recognition

  • Microfluidic devices

  • Capillary electrophoresis

  • Capillary-array devices

  • Optical sensors

  • Ion sensors

  • Ionophores

Research subject summary

  • 化学センサー用新規機能分子・ナノ材料の開発

  • マイクロ・ナノ分析デバイスの開発

  • 新しい化学センシング・分離機構の設計

Research Career

  • 高機能材料・マイクロデバイスを用いた先端計測・分析法の開発

    化学センシング、マイクロチップ、キャピラリー電気泳動 

Professional Memberships

  • Society fo Chemistry and Micro-Nano Systems

    2000.03 - Now   Domestic

  • Japan Society for Analytical Chemistry

    1996.04 - Now   Domestic

  • Chemical Society of Japan

    1996.04 - Now   Domestic

Committee Memberships (off-campus)

  • 第102年会会場・総務小委員会(総務)委員   日本化学会  

    2021 - 2022.03 

  • 第70年会実行委員   日本分析化学会  

    2021 

  • 奨励賞候補者選考委員会委員   近畿分析技術研究懇話会  

    2021 

  • 特別実行委員長   日本分析化学会分析イノベーション交流会主催ものつくり技術交流会2021in関西~分析に役立つ基礎技術~  

    2021 

  • 令和2年度JASISコンファレンス分科会委員   一般社団法人 日本分析機器工業会  

    2020.04 - 2021.03 

  • 功労賞・技術功績賞審査委員長   日本分析化学会  

    2020.03 - 2022.02 

  • 令和1年度JASISコンファレンス分科会委員   一般社団法人 日本分析機器工業会  

    2019.04 - 2020.03 

  • 平成30年度JASISコンファレンス分科会委員   一般社団法人 日本分析機器工業会  

    2018.04 - 2019.03 

  • 平成29年度JASISコンファレンス分科会委員   一般社団法人 日本分析機器工業会  

    2017.04 - 2018.03 

  • 平成28年度JASISコンファレンス分科会委員   一般社団法人 日本分析機器工業会  

    2016.04 - 2017.03 

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Awards

  • Outstanding Reviewer 2022 Sensors and Diagnostics

    2023.03   The Royal Society of Chemistry  

  • 2022 ACS PUBLICATIONS PEER REVIEWER CERTIFICATE OF RECOGNITION & APPRECIATION

    2023.03   ACS Publications  

  • The Japan Society for Analytical Chemistry Award

    2022.09   The Japan Society for Analytical Chemistry   Development and research of basic technology for high-performance sensing materials and multifunctional integrated micro-analytical devices

  • Best Paper Award in BUNSEKI KAGAKU 2021

    Yuko Kawai, Akihiro Shirai, Masaya Kakuta, Kotaro Idegami, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    2022.09   Japan Society for Analytical Chemistry   Development of the Single-step Bioassay Microdevice using Reagent Immobilization Method based on Inkjet Printing

  • Outstanding Reviewer for Analyst in 2020

    2021.03   The Royal Society of Chemistry  

  • Outstanding Reviewer for Analyst in 2019

    2020.03   The Royal Society of Chemistry  

  • Yagami Award

    2019.09   Faculty of Science and Technology, Keio University   Research and development of basic technology for multi-function integrated microanalytical devices for diagnostic / biological research applications

  • Outstanding Reviewer for Analyst in 2018

    2019.03   The Royal Society of Chemistry  

  • Outstanding Reviewer for Analyst in 2017

    2018.03   The Royal Society of Chemistry  

  • TOP REFEREE 2008

    2009.01   Analytica Chimica Acta (Elsevier)  

  • 化学とマイクロナノシステム研究会(CHEMINAS) 奨励賞

    2008.05   化学とマイクロナノシステム研究会  

  • TOP REFEREE 2007

    2008.02   Journal of Chromatography A (Elsevier)  

  • TOP REFEREE 2006

    2007.02   Analytica Chimica Acta (Elsevier)  

  • 奨励賞

    2004.09   日本分析化学会  

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Job Career (off-campus)

  • Osaka Metropolitan University   Graduate School of Engineering

    2022.04 - Now

  • Osaka Prefecture University   Graduate School of Engineering Division of Materials Science and Engineering

    2010.04 - 2022.03

  • Osaka Prefecture University   Graduate School of Engineering Division of Materials Science and Engineering

    2007.04 - 2010.03

  • University of Hyogo   Graduate School of Material Science

    2004.04 - 2007.03

  • Himeji Institute of Technology   Graduate School of Science   Associate Professor

    2003.01 - 2004.03

  • The University of Tokyo   The Graduate School of Engineering Department of Applied Chemistry

    1999.04 - 2002.12

  • Keio University   Faculty of Science and Technology Department of Applied Chemistry

    1996.04 - 1999.03

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Papers

  • Combining TiO<inf>2</inf> photonic crystals and reagents enhances the sensitivity of endotoxin detection

    Nishitsuji R.

    Chemical Papers   77 ( 1 )   611 - 615   2023.01( ISSN:03666352

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  • Aptamer Selection Based on Microscale Electrophoretic Filtration Using a Hydrogel-Plugged Capillary Device.

    Junku Takao, Reina Nagai, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi

    Molecules (Basel, Switzerland)   27 ( 18 )   2022.09

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    This study reports a novel aptamer selection method based on microscale electrophoretic filtration. Aptamers are versatile materials that recognize specific targets and are attractive for their applications in biosensors, diagnosis, and therapy. However, their practical applications remain scarce due to issues with conventional selection methods, such as complicated operations, low-efficiency separation, and expensive apparatus. To overcome these drawbacks, a selection method based on microscale electrophoretic filtration using a capillary partially filled with hydrogel was developed. The electrophoretic filtration of model target proteins (immunoglobulin E (IgE)) using hydrogel, the electrokinetic injection of DNAs to interact with the trapped proteins, the elimination of DNAs with weak interactions, and the selective acquisition of aptamer candidates with strong interactions were successfully demonstrated, revealing the validity of the proposed concept. Two aptamer candidates for IgE were obtained after three selection cycles, and their affinity for the target was confirmed to be less than 1 nM based on their dissociation constant (KD) values. Therefore, the proposed method allows for the selection of aptamers with simple operations, highly effective separation based on electrophoresis and filtration, and a relatively cheap apparatus with disposable devices.

    DOI: 10.3390/molecules27185818

    PubMed

  • Fabrication of Gold Nanostructures on Quartz Crystal Microbalance Surface Using Nanoimprint Lithography for Sensing Applications.

    Ryosuke Nishitsuji, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Micromachines   13 ( 9 )   2022.08( ISSN:2072-666X

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    A quartz crystal microbalance (QCM) is a sensor that uses the piezoelectric properties of quartz crystals sandwiched between conductive electrodes. Localized surface plasmon resonance (LSPR) is an analytical technique that uses the collective vibration of free electrons on metal surfaces. These measurements are known as analysis techniques that use metal surfaces and have been applied as biosensors because they allow for the label-free monitoring of biomolecular binding reactions. These measurements can be used in combination to analyze the reactions that occur on metal surfaces because different types of information can be obtained from them. However, as different devices are used for these measurements, the results often contain device-to-device errors and are not accurately evaluated. In this study, we directly fabricated gold nanostructures on the surface of a QCM to create a device that can simultaneously measure the mass and refractive index information of the analyte. In addition, the device could be easily fabricated because nanoimprint lithography was used to fabricate gold nanostructures. As a proof of concept, the nanoparticle adsorption on gold nanostructures was evaluated, and it was observed that mass and refractive index information were successfully obtained without device-to-device errors.

    DOI: 10.3390/mi13091430

    PubMed

  • Gold Nanocone Array with Extensive Electromagnetic Fields for Highly Reproducible Surface-Enhanced Raman Scattering Measurements.

    Satoko Fujiwara, Daiki Kawasaki, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Micromachines   13 ( 8 )   2022.07( ISSN:2072-666X

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Surface-enhanced Raman scattering (SERS) is a technique used to distinguish the constitution of disease-related biomarkers in liquid biopsies, such as exosomes and circulating tumor cells, without any recognition elements. Previous studies using metal nanoparticle aggregates and angular nanostructures have achieved the detection of various biomarkers owing to strong hot spots and electromagnetic (EM) fields by localized surface plasmon resonance (LSPR). Although these SERS platforms enable significant enhancement of Raman signals, they still have some problems with the fabrication reproducibility of platforms in obtaining reproducible SERS signals. Therefore, highly reproducible fabrication of SERS platforms is required. Here, we propose the application of a polymer-based gold (Au) nanocone array (Au NCA), which extensively generates an enhanced EM field near the Au NCA surface by LSPR. This approach was experimentally demonstrated using a 785 nm laser, typically used for SERS measurements, and showed excellent substrate-to-substrate reproducibility (relative standard deviation (RSD) &lt; 6%) using an extremely simple fabrication procedure and very low laser energy. These results proved that a Au NCA can be used as a highly reproducible SERS measurement to distinguish the constitution of biomarkers.

    DOI: 10.3390/mi13081182

    PubMed

  • Quantum dots / TiO2 hybrid photonic crystal: Fabrication and application for highly sensitive and visible region-responsive biosensor

    Shotaro Ueda, Daiki Kawasaki, Ryosuke Nishitsuji, Hirotaka Yamada, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    MICROELECTRONIC ENGINEERING   263   2022.07( ISSN:0167-9317

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    Publishing type:Research paper (scientific journal)  

    In this work, by combining a photonic crystal (PhC) and highly luminescent material, we developed an extremely sensitive PhC sensor that allows detection by single wavelength fluorescence intensity change in the visible wavelength region. We fabricated a titanium dioxide (TiO2) PhC containing quantum dots (QDs) inside the PhC structure and confirmed that the fluorescence intensity of the QDs was enhanced. Fluorescence enhancement occurs because of the total reflection and light confinement of the QD fluorescence inside the TiO2 PhC structure. The response to changes in the surrounding refractive index and protein adsorption was confirmed. Furthermore, for biosensing applications, troponin T (TnT), an acute myocardial marker, was determined using an antigen -antibody reaction. It was found that a TnT level of 100 fg/mL, which is considerably less than the cutoff value of 100 pg/mL, was successfully detected. The lower limit of detection (LOD) was found to be 58.2 fg/mL, which is 1/40 of that of the conventional enzyme-linked immunosorbent assay (ELISA) method (LOD: 2 pg/mL). It was demonstrated that the present TiO2 PhC-containing QDs can be used as a sensor device for the highly sensitive detection and determination of disease biomarkers.

    DOI: 10.1016/j.mee.2022.111842

  • Evaluation of the interactions between oligonucleotides and small molecules by partial filling-nonequilibrium affinity capillary electrophoresis.

    Mitsuno E, Endo T, Hisamoto H, Sueyoshi K

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry   38 ( 6 )   851 - 859   2022.06( ISSN:0910-6340

  • 【高感度解析に寄与する分離分析技術】デジタル分子ふるい電気泳動用キャピラリーデバイスの開発

    末吉 健志, 松田 景太, 遠藤 達郎, 久本 秀明

    分析化学   71 ( 6 )   325 - 331   2022.06( ISSN:0525-1931

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    デジタル電気泳動法は、各種機能性ヒドロゲルに対する試料分子の通過・非通過を利用した分離・濃縮法として近年注目されているが、ヒドロゲル充填デバイス作製の煩雑さが大きな課題であった。そこで、本研究では、簡易デバイスを用いたデジタル電気泳動技術の開発と、機能性ヒドロゲルによるタンパク質の分離・濃縮評価のため、異種ポリアクリルアミドゲル連続充填キャピラリーデバイスを作製し、デジタル分子ふるい電気泳動の基礎評価を行った。その結果、プレポリマー溶液中アクリルアミド総濃度(%T)と通過・非通過タンパク質の分子量との相関が明らかとなった。また、濃縮効率評価の結果、最大で約180倍の高感度化が確認された。さらに、5、15、25%Tポリアクリルアミドゲル連続充填デバイスを用いたデジタル分子ふるい電気泳動によって、分子量が異なるタンパク質の分離と濃縮が同時に達成された。以上の結果から、作製したデバイスを用いた簡便・高分離能・高感度なデジタル電気泳動が実証された。(著者抄録)

  • Wavelength-tunable dual-band edge plasmon mode based on gold edge-hole plasmonic nanostructure

    Hirotaka Yamada, Daiki Kawasaki, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    RESULTS IN PHYSICS   37   2022.06( ISSN:2211-3797

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    Publishing type:Research paper (scientific journal)  

    Metal nanostructures have attracted attention because of their unique optical characteristics derived from localized surface plasmon resonance. In this study, gold nanoholes with edge structures were designed and characterized to possess two plasmon bands at different wavelengths. These bands were assigned to the plasmon modes that generated a concentrated electric field at the tip of the individual edge structures. Furthermore, wavelengths of these bands were independently tuned from the visible to near-infrared wavelengths with shape and size of the gold nanoholes and edge structures as variables. To demonstrate wavelength tuning, a gold nanostructure with edge plasmon bands at wavelengths of approximately 785 and 1064 nm (typical laser wavelengths for surface-enhanced Raman scattering and optical tweezers, respectively) was successfully obtained via optical simulation. The results indicate that the structure designed in this study provides a guideline for the design of optical devices with efficient multiple optical functions.

    DOI: 10.1016/j.rinp.2022.105541

  • Au nanorods-TiO2 photonic crystal plasmonic-photonic hybrid sensor for label-free detection and identification of DNA molecules with single nucleotide polymorphisms

    Daiki Kawasaki, Hirotaka Yamada, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    SENSORS AND ACTUATORS B-CHEMICAL   361   2022.06( ISSN:09254005

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    Publishing type:Research paper (scientific journal)  

    Highly sensitive optical sensors for label-free detection of DNA must have high usability and wide applicability for achieving advances in the medical and biological science fields. However, current sensors do not simultaneously satisfy these criteria. Here, we developed a simple, cost-effective, and sensitive plasmonic-photonic hybrid sensor using gold nanorods as nanoantennae and a photonic crystal slab (PCS) as a microcavity. In this report, we performed simple microscopic detection and identification of DNA molecules with Alzheimer's disease-associated single nucleotide polymorphisms, which is an important indicator for early diagnosis. The PCS was fabricated using nanoimprint lithography; the gold nanorods were controllably coupled with the PCS. The device sensitivity was experimentally assessed by controlling the plasmonic-photonic coupling resonance, which was enabled by tuning the PCS-coupled AuNR amounts. The sensitivity was also investigated by theoretical modeling of the hybrid sensor, based on the temporal coupled mode theory (TCMT). The TCMT-based theoretical model well explained the experimental results. In a demonstration of ultrasensitive detection of DNA, the identification of single nucleotide mismatched DNA molecules (similar to 1 pM) and detection of several DNA molecules (similar to 1000), with a limit of detection of 5.9 aM, were successfully achieved. This sensor may thus have wide applications for DNA analysis.

    DOI: 10.1016/j.snb.2022.131747

  • Chloride ion-selective dye liquid nanoemulsion: improved sensor performance due to intermolecular interactions between dye and ionophore.

    Maki K, Oishi R, Mizuta T, Sueyoshi K, Endo T, Hisamoto H

    The Analyst   147 ( 8 )   1529 - 1533   2022.04( ISSN:0003-2654

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  • Imprinted Photonic Crystal-Film-Based Smartphone-Compatible Label-Free Optical Sensor for SARS-CoV-2 Testing.

    Kawasaki D, Yamada H, Sueyoshi K, Hisamoto H, Endo T

    Biosensors   12 ( 4 )   2022.03

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  • Fabrication of Metal-Insulator-Metal Nanostructures Composed of Au-MgF<sub>2</sub>-Au and Its Potential in Responding to Two Different Factors in Sample Solutions Using Individual Plasmon Modes.

    Yamada H, Kawasaki D, Sueyoshi K, Hisamoto H, Endo T

    Micromachines   13 ( 2 )   2022.02

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  • Modulating Optical Characteristics of Nanoimprinted Plasmonic Device by Re-Shaping Process of Polymer Mold.

    Hirotaka Yamada, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Micromachines   12 ( 11 )   2021.10

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Metal nanostructures exhibit specific optical characteristics owing to their localized surface plasmon resonance (LSPR) and have been studied for applications in various optical devices. The LSPR property strongly depends on the size and shape of metal nanostructures; thus, plasmonic devices must be designed and fabricated according to their uses. Nanoimprint lithography (NIL) is an effective process for repeatedly fabricating metal nanostructures with controlled sizes and shapes and require optical properties. NIL is a powerful method for mass-producible, low-cost, and large-area fabrication. However, the process lacks flexibility in adjusting the size and shape according to the desirable optical characteristics because the size and shape of metal nanostructures are determined by a single corresponding mold. Here, we conducted a re-shaping process through the air-plasma etching of a polymer's secondary mold (two-dimensional nanopillar array made of cyclo-olefin polymer (COP)) to modulate the sizes and shapes of nanopillars; then, we controlled the spectral characteristics of the imprinted plasmonic devices. The relationship between the structural change of the mold, which was based on etching time, and the optical characteristics of the corresponding plasmonic device was evaluated through experiments and simulations. According to evaluation results, the diameter of the nanopillar was controlled from 248 to 139 nm due to the etching time and formation of a pit structure. Consequently, the spectral properties changed, and responsivity to the surrounding dielectric environment was improved. Therefore, plasmonic devices based on the re-shaped COP mold exhibited a high responsivity to a refractive index of 906 nm/RIU at a wavelength of 625 nm.

    DOI: 10.3390/mi12111323

    PubMed

  • Enzyme-responsive fluorescent nanoemulsion based on lipophilic dye liquid.

    Ryoutarou Oishi, Kaho Maki, Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    The Analyst   146 ( 13 )   4121 - 4124   2021.06( ISSN:0003-2654

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    An enzyme-responsive fluorescent nanoemulsion (NE) based on lipophilic dye liquid (LDL) was developed for alkaline phosphatase (ALP). The response mechanism of the NE involved enzymatic reactions and simultaneous extraction of anions. The LDL-based NE exhibited 3.8 times higher sensitivity than plasticizer-based conventional NE. Detection limit and response range were 2.7 (U L-1) and 5-50 (U L-1), respectively. The response time was reduced to less than half that of the LDL-based membrane.

    DOI: 10.1039/d1an00447f

    PubMed

  • Fractionation of Single-stranded DNAs with/without Stable Preorganized Structures Using Capillary Sieving Electrophoresis for Aptamer Selection.

    Masahide Wada, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry   37 ( 6 )   799 - 802   2021.06

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:Domestic journal  

    Aptamers, single-stranded DNAs/RNAs with a strong and specific interaction towards a target molecule, have wide applications in the fields of medicine and biosensors. In conventional aptamer selection methods, it is difficult to obtain "preorganized" and/or "induced-fit" type of aptamers selectively. In this study, separation and fractionation of single-stranded DNAs with/without stable preorganized structures were carried out using capillary sieving electrophoresis. The fractionated DNAs showed different mobilities and thermodynamic stabilities of their secondary structures; this outcome is deemed to be necessary for the synthesis of novel aptasensors with a desirable sensing mechanism.

    DOI: 10.2116/analsci.21C003

    PubMed

  • Single-Step Trypsin Inhibitor Assay on a Microchannel Array Device Immobilizing Enzymes and Fluorescent Substrates by Inkjet Printing.

    Yuko Kawai, Kotaro Idegami, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry   37 ( 10 )   1473 - 1476   2021.04( ISSN:0910-6340

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:Domestic journal  

    DOI: 10.2116/analsci.21N011

    PubMed

  • Direct measurement of initial rate of enzyme reaction by electrokinetic filtration using a hydrogel-plugged capillary device.

    Junku Takao, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry   37 ( 10 )   1439 - 1446   2021.04( ISSN:0910-6340

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:Domestic journal  

    DOI: 10.2116/analsci.21P067

    PubMed

  • Simple Approach for Fluorescence Signal Amplification Utilizing a Poly(vinyl alcohol)-Based Polymer Structure in a Microchannel.

    Keine Nishiyama, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Hideaki Hisamoto, Manabu Tokeshi

    ACS omega   6 ( 12 )   8340 - 8345   2021.03

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Analytical methods with fluorescence detection are in widespread use for detecting low abundance analytes. Here, we report a simple method for fluorescence signal amplification utilizing a structure of an azide-unit pendant water-soluble photopolymer (AWP) in a microchannel. The AWP is a poly(vinyl alcohol)-based photocross-linkable polymer, which is often used in biosensors. We determined that the wall-like structure of the AWP (AWP-wall) constructed in a microchannel functioned as an amplifier of a fluorescence signal. When a solution of fluorescent molecules was introduced into the microchannel having the AWP-wall, the fluorescent molecules accumulated inside the AWP-wall by diffusion. Consequently, the fluorescence intensity inside the AWP-wall increased locally. Among the fluorescent molecules considered in this paper, 9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) (DDAO) showed the highest efficiency of fluorescence signal amplification. We prepared a calibration curve for DDAO using the fluorescence intensity inside the AWP-wall, and the sensitivity was 5-fold that for the microchannel without the AWP-wall. This method realizes the improved sensitivity of fluorescence detection easily because the fluorescence signal was amplified only by injecting the solution into the microchannel having the AWP-wall. Furthermore, since this method is not limited to only the use of microchannel, we expect it to be applicable in various fields.

    DOI: 10.1021/acsomega.1c00057

    PubMed

  • Lipophilic Fluorescent Dye Liquids: Förster Resonance Energy Transfer-Based Fluorescence Amplification for Ion Selective Optical Sensors Based on a Solvent Polymeric Membrane.

    Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Analytical chemistry   93 ( 9 )   4143 - 4148   2021.03( ISSN:0003-2700

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Optical sensors based on solvent polymeric membranes have the potential to measure analytes present in an aqueous solution through the development of a tailored method for a specific target. However, limits in the concentrations of the component dyes have prevented improvements in sensitivity. We propose a Förster resonance energy transfer (FRET)-based fluorescence amplification system for ion-selective optical sensors using a highly fluorescent liquid material composed of a lipophilic phosphonium cation and a pyrene modifying sulfonate anion ([P66614][HP-SO3]), as both the plasticizer and donor, in addition to a combination of the lipophilic phosphonium cation and the fluorescein dodecyl ester anion ([P66614][12-FL]) as the fluorescent sensing dye acceptor. For ion extraction-based sensing, the donor and acceptor were retained in the plasticized PVC membrane with negligible leaching upon exposure to acidic and basic aqueous solutions. Systematic investigation of the donor and acceptor ratios clarified the effect of the amplification factor and the sensitivity of the sensor. At an acceptor doping level of 0.5 mol % (vs donor), an approximately 22-fold higher sensitivity was obtained compared to that of a conventional PVC membrane optical sensor. During ion measurement based on the coextraction of protons and anions, selectivity following the Hofmeister order was observed, which was controlled by the addition of ionophores. The proposed FRET system based on a lipophilic fluorescent liquid material has the potential to significantly improve the sensitivities of optical sensors using solvent polymeric membranes with high selectivities for various target analytes.

    DOI: 10.1021/acs.analchem.0c05007

    PubMed

  • 【化学センシングの方法論・デバイス開発の新展開】インクジェットプリンティングによる試薬固定化法を利用する1ステップバイオアッセイマイクロデバイスの開発

    河相 優子, 白井 亮洋, 角田 正也, 井手上 公太郎, 末吉 健志, 遠藤 達郎, 久本 秀明

    分析化学   70 ( 3 )   125 - 131   2021.03( ISSN:0525-1931

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    本研究では、インクジェットプリンティングを用いて一つのポリジメチルシロキサン(Poly(dimethylsiloxane)、PDMS)製マイクロ流路内に2種類の反応試薬を固定化した1ステップ均一系競合型バイオアッセイマイクロデバイスを開発した。インクジェットプリンターは試薬をナノリットルサイズの液滴として正確な量を位置選択的に吐出するため、互いに反応する2種類の試薬を混ざることなく微少空間内に独立して固定化できる。そのため、先行研究でデバイスを作製する際に課題となった、相互に反応する試薬を固定化した二つのマイクロ流路を精密に組み合わせる操作が不要となる。本研究ではその一例として、ビオチン固定化酸化グラフェン(BG)と蛍光標識ストレプトアビジン(F-SA)を1本のマイクロ流路内に固定してビオチンアッセイマイクロデバイスを試作し、基礎検討として試薬の固定化位置や試薬及び添加剤種の評価を行った。その結果、液滴を流路壁面寄りに吐出すると再現性よく流路底面の両サイドに試薬固定できること、添加剤としてトレハロースが適すること及び、試料導入操作のみでの1ステップ検出で、検出下限は0.68ng mL-1となり、先行研究と比較して約1/600倍改善することを明らかにした。(著者抄録)

  • Highly sensitive optical ion sensor with ionic liquid-based colorimetric membrane/photonic crystal hybrid structure

    Daiki Kawasaki, Ryoutarou Oishi, Nao Kobayashi, Tatsumi Mizuta, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Scientific Reports   10 ( 1 )   16739   2020.12

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    Publishing type:Research paper (scientific journal)  

    © 2020, The Author(s). An ionic liquid-based thin (~ 1 µm) colorimetric membrane (CM) is a key nano-tool for optical ion sensing, and a two-dimensional photonic crystal slab (PCS) is an important nano-platform for ultimate light control. For highly sensitive optical ion sensing, this report proposes a hybrid of these two optical nano-elements, namely, a CM/PCS hybrid. This structure was successfully fabricated by a simple and rapid process using nanoimprinting and spin-coating, which enabled control of the CM thickness. Optical characterization of the hybrid structure was conducted by optical measurement and simulation of the reflection spectrum, indicating that the light confined in the holes of the PCS was drastically absorbed by the CM when the spectrum overlapped with the absorption spectrum of the CM. This optical property obtained by the hybridization of CM and PCS enabled drastic improvement in the absorption sensitivity in Ca ion sensing, by ca. 78 times compared to that without PCS. Experimental and simulated investigation of the relation between the CM thickness and absorption sensitivity enhancement suggested that the controlled light in the PCS enhanced the absorption cross-section of the dye molecules within the CM based on the enhanced local density of states. This highly sensitive optical ion sensor is expected to be applied for micro-scale bio-analysis like cell-dynamics based on reflectometric Ca ion detection.

    DOI: 10.1038/s41598-020-73858-8

    PubMed

  • A lipophilic ionic liquid-based dye for anion optodes: importance of dye lipophilicity and application to heparin measurement

    Tatsumi Mizuta, Shu Takai, Toshiki Nishihata, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    The Analyst   145 ( 16 )   5430 - 5437   2020.08( ISSN:0003-2654

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    Publishing type:Research paper (scientific journal)  

    Herein, a fully lipophilic ionic liquid (IL) comprising a lipophilic fluorescein anion and a trihexyltetradecylphosphonium cation was synthesized and used as the plasticizer for a plasticized poly(vinyl chloride) (PVC) membrane optode. Systematic investigation of the alkyl chain length of the fluorescein anion proved the significance of lipophilicity for obtaining the reversible absorbance measurements. A PVC membrane fabricated with the synthesized lipophilic IL was observed to comprise an unusually high dye concentration (915 mmol kg-1) and exhibited good sensitivity as well as response time in its sensor performance. The sensitivity of the presented PVC membrane was 26-fold higher than that of a conventional optode membrane with the same membrane thickness and the same lipophilic dye of typical dye content (1 wt%). The response time was observed to be >120-fold faster by using a significantly thinner PVC membrane (approx. 140 nm). Heparin is known to be a polyanionic anticoagulant, and the presented PVC membrane exhibited an extremely fast response (20-150 seconds) to the heparin in diluted serum within the required concentration region. Thus, the lipophilic IL-based dye could significantly improve the sensor performance in conventional optodes, especially for an analyte showing slow diffusion, such as macromolecular heparin.

    DOI: 10.1039/d0an00335b

    PubMed

  • Outstanding Reviewers for Analyst in 2019

    Hugh Byrne, Lingxin Chen, Yu Chung Chang, Jeremy Driskell, Ning Gan, Hideaki Hisamoto, Juewen Liu, Francis Martin, Muhammad Shiddiky, Chun Yang Zhang

    Analyst   145 ( 12 )   4068   2020.06( ISSN:0003-2654

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1039/d0an90052d

    PubMed

  • Size sorting of exosomes by tuning the thicknesses of the electric double layers on a micro-nanofluidic device

    Satoko Fujiwara, Kyojiro Morikawa, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi

    Micromachines   11 ( 5 )   2020.05

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    Publishing type:Research paper (scientific journal)  

    © 2020 by the authors. Exosomes, a type of extracellular vesicle with a diameter of 30-150 nm, perform key biological functions such as intercellular communication. Recently, size sorting of exosomes has received increasing attention in order to clarify the correlation between their size and components. However, such sorting remains extremely difficult.Here, we propose to sort their size by controlling their electrokineticmigration in nanochannels in a micro-nanofluidic device, which is achieved by tuning the thickness of the electric double layers in the nanochannels. This approach was demonstrated experimentally for exosomes smaller than 250 nm. Using different running buffer concentrations (1 × 10-3, 1 × 10-4, and 1 × 10-5 M), most of the exosomes larger than 140, 110, and 80 nm were successfully cut off at the downstream of the nanochannels, respectively. Therefore, it is clarified that the proposed method is applicable for the size sorting of exosomes.

    DOI: 10.3390/MI11050458

    PubMed

  • Inkjet Printing-Based Immobilization Method for a Single-Step and Homogeneous Competitive Immunoassay in Microchannel Arrays.

    Yuko Kawai, Akihiro Shirai, Masaya Kakuta, Kotaro Idegami, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Frontiers in chemistry   8   612132 - 612132   2020

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    In this study, we report an inkjet printing-based method for the immobilization of different reactive analytical reagents on a single microchannel for a single-step and homogeneous solution-based competitive immunoassay. The immunoassay microdevice is composed of a poly(dimethylsiloxane) microchannel that is patterned using inkjet printing by two types of reactive reagents as dissolvable spots, namely, antibody-immobilized graphene oxide and a fluorescently labeled antigen. Since nanoliter-sized droplets of the reagents could be accurately and position-selectively spotted on the microchannel, different reactive reagents were simultaneously immobilized onto the same microchannel, which was difficult to achieve in previously reported capillary-based single-step bioassay devices. In the present study, the positions of the reagent spots and amount of reagent matrix were investigated to demonstrate the stable and reproducible immobilization and a uniform dissolution. Finally, a preliminary application to a single-step immunoassay of C-reactive protein was demonstrated as a proof of concept.

    DOI: 10.3389/fchem.2020.612132

    PubMed

  • Enzyme-responsive fluorescent ionic liquid

    Ryoutarou Oishi, Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Analytical Sciences   36 ( 2 )   143 - 145   2020( ISSN:0910-6340

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    © The Japan Society for Analytical Chemistry. Herein, we describe the development of a novel material, "enzyme-responsive fluorescent ionic liquid", which enabled a highly sensitive detection of alkaline phosphatase (ALP). We prepared a plasticized poly(vinyl chloride) (PVC) membrane using this new material as a plasticizer and quantified ALP in aqueous solutions. Preliminary results suggested that the PVC membrane responded to ALP at an interface between the membrane and the sample solution with anion extraction to maintain electroneutrality in the membrane phase. The developed PVC membrane showed an approximately six-times higher sensitivity than the conventional membrane, thereby demonstrating highly sensitive ALP detection. These results suggested the potential applicability of the proposed membrane for highly sensitive protein detection by using ALPlabeled antibodies.

    DOI: 10.2116/analsci.19C013

    PubMed

  • An ionic liquid composed of purely functional sensing molecules: A colorimetrically calcium responsive ionic liquid

    Yusuke Niwa, Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Analyst   144 ( 23 )   6858 - 6861   2019.12( ISSN:0003-2654

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    Publishing type:Research paper (scientific journal)  

    © The Royal Society of Chemistry 2019. A highly lipophilic ionic liquid (IL) consisting of stoichiometrically equal amounts of two purely functional chemical sensing molecules, an anionic calcium ionophore and a cationic dye, was synthesized for the first time, and used as a component for a poly(vinyl chloride)-based or neat liquid membrane optical sensor. This is the first report of an IL consisting of purely functional chemical sensing molecules, which is completely different from the previously reported ionic liquids typically consisting of imidazolium or other lipophilic cations. Since the present IL contained an extremely high concentration of dyes, the IL-based sensor showed dramatically enhanced sensitivity (13 times higher compared to that of a conventional sensor), and fully reversible and selective response to Ca2+. Preliminary investigation on the unique response characteristics of the present liquid membrane IL-based sensor was performed with the responses to Ca2+ and Na+.

    DOI: 10.1039/c9an01769k

    PubMed

  • TiN-contained polymer-metal core-shell structured nanocone array: Engineering of sensor performance by controlling plasmonic properties

    Daiki Kawasaki, Kenichi Maeno, Hirotaka Yamada, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Sensors and Actuators, B: Chemical   299   2019.11( ISSN:0925-4005

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    Publishing type:Research paper (scientific journal)  

    © 2019 Elsevier B.V. Metal nanostructures have great potential for optical label-free biosensors based on localized surface plasmon resonance (LSPR). The sensitivity of a metal nanostructure-based label-free biosensor (i.e., plasmonic sensor) depends on its plasmonic properties, which suffer a decrease in sensitivity by energy losses in the metal material. Here, we demonstrate an approach to improve the plasmonic properties of metal nanostructures by controlling the carrier density in the base polymer material using titanium nitride (TiN). It is expected that the light energy absorbed by TiN is converted into excitons, and it will assist LSPs in the metal nanostructure; thus, the losses of the metal material are compensated by the excitons excited in TiN. We designed a TiN-contained polymer-metal/core-shell structured nanocone array (NCA), comprising TiN nanoparticles (NPs) in a polymer core and metal shell (Au or Ag), and realized improvement of the refractive index (RI) sensitivity of a label-free biosensor by optimizing the TiN-contained polymer composition. As a result, the TiN-contained polymer-metal NCA, with a TiN NP concentration of 10 wt% in the polymer core, had a 1.5-fold higher RI sensitivity than that of the same NCA without TiN NPs. The results of the resistance measurement of the metal surface with the TiN NP-contained polymer (10 wt%) under light exposure suggest the conversion of exposed light into LSPs of metal via TiN. It is suggested that plasmonic properties and sensor performances can be improved by the presented approach. Moreover, in DNA hybridization detection, an extremely low limit of detection of 117.5 fM was achieved.

    DOI: 10.1016/j.snb.2019.126932

  • Core-Shell-Structured Gold Nanocone Array for Label-Free DNA Sensing

    Daiki Kawasaki, Hirotaka Yamada, Kenichi Maeno, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    ACS Applied Nano Materials   2 ( 8 )   4983 - 4990   2019.08

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    Publishing type:Research paper (scientific journal)  

    Copyright © 2019 American Chemical Society. The refractive index sensitivity of a localized surface plasmon resonance (LSPR) sensor is correlated to an enhanced local electromagnetic (EM) field originating from noble metal nanostructures. Here, we demonstrated that extensive EM field enhancement by a gold (Au) nanocone array (AuNCA) allowed highly sensitive and label-free detection of biomolecules in the visible wavelength spectrum. The AuNCA consisted of a polymer core and an Au shell, which was fabricated by using simple and inexpensive nanoimprint lithography. Under LSPR excitation, AuNCA absorbs visible light of a specific wavelength and extensively enhances the EM field near its surface. It was shown that AuNCA had high refractive index sensitivity (417.5 nm/RIU) because of the large distribution of the enhanced EM field, covering a large surface of the NCA. Moreover, in DNA hybridization detection, a very low limit of detection of 161 fM was achieved, and 1-base mismatch DNA was successfully discriminated by using AuNCA.

    DOI: 10.1021/acsanm.9b00930

  • Quantitative analysis of liquid crystal-based immunoassay using rectangular capillaries as sensing platform

    Jhih Wei Huang, Hideaki Hisamoto, Chih Hsin Chen

    Optics Express   27 ( 12 )   17080 - 17090   2019.06

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    © 2019 Optical Society of America. In past studies, liquid crystal (LC)-based immunoassays were accomplished by fabricating an LC cell with two pieces of glass slides after immunobinding, which makes the determination of the immunoassay not in real-time and requires trained personnel. Herein, we developed the LC-based immunoassay by using rectangular capillaries as the substrate for immunobinding. The inner surface of rectangular capillaries was decorated with a long alkyl saline, dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride (DMOAP), followed by immobilization of human serum albumin (HSA) as the probe. In this situation, the orientation of LC was homeotropic and dark LC image was observed under polarized light. When the solution containing anti-human serum albumin (anti-HSA) were dispensed into the capillary through capillary action, the specific immunobinding between HSA and anti-HSA formed an immunocomplex on the inner surface of capillary, which disrupted the original orientation of LC and led to a dark-to-bright transition of the LC images. The quantification of anti-HSA can be achieved by measuring the length of the bright LC image in the rectangular capillary. By using this immunoassay, the limit of detection (LOD) for anti-HSA is 1 μg/mL, and it did not respond to HSA and anti-human immunoglobulin G (anti-h-IgG). On the other hand, the diversity of the LC-based immunoassay can be extended for HSA detection when we immobilized anti-HSA in the capillary. Because the post-fabrication of LC cell was waived by using rectangular capillaries to develop the LC-based immunoassay, it is more convenient for users to handle and collect more reliable data. Moreover, the results of the immunoassay were visualized through naked-eye and could be recorded by a smartphone; it is more suitable for portable and point-of-care applications.

    DOI: 10.1364/OE.27.017080

    PubMed

  • Graphene/polyethylene glycol hybrids for single-step immunoassay microdevice

    Akihiro Shirai, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    FlatChem   13   34 - 39   2019.01

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    Publishing type:Research paper (scientific journal)  

    © 2018 Elsevier B.V. In this study, we developed a novel particle-type material comprised of graphene and polyethylene glycol (PEG) to solve the slow response problem of the previously reported microdevice for single-step immunoassay. Characterization of these graphene/PEG hybrids (GPH) was carried out using a transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, and fluorescence analysis. We found that PEG attached to graphene surfaces behave like molecular sieves to separate free fluorescently-labeled antibodies from antigen-bound antibody immunocomplexes. The free antibody is PEG-permeable and directly adsorbs onto the graphene surface, where fluorescence is quenched by fluorescence resonance energy transfer. On the contrary, immunocomplexes are PEG-impermeable; thus, as the concentration of the antigen increases, the concentration of PEG-impermeable immunocomplexes also increases; consequently, increasing the fluorescence intensity of the solution. After characterizing GPH, single-step immunoassay microdevices were prepared by separately immobilizing the GPH and fluorescently-labeled antibodies on two independent microchannel arrays and combining them. Finally, we tested the proposed immunoassay microdevices with various known concentrations of C-reactive protein (CRP), an inflammatory marker protein, and successfully measured CRP concentration within an approximate time of 2 min by monitoring the fluorescence intensity change.

    DOI: 10.1016/j.flatc.2018.12.003

  • TiN-contained polymer-metal core-shell structured nanocone array: Engineering of sensor performance by controlling plasmonic properties Reviewed

    Daiki Kawasaki, Kenichi Maeno, Hirotaka Yamada, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Sens. Actuators B.   299   2019

  • Au "Edged Hole Array" for Sensor Application Reviewed

    Hirotaka Yamada, Daiki Kawasaki, Chigusa Inoue, Kenichi Maeno, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Journal of Photopolymer Science and Technology 雑誌   32   101 - 105   2019

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    Kind of work:Joint Work  

  • Development of a rapid and highly sensitive plasticized PVC membrane Optode utilizing an ionic liquid material composed of bromothymol blue

    Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Bunseki Kagaku   68 ( 12 )   945 - 951   2019( ISSN:0525-1931

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    Publishing type:Research paper (scientific journal)  

    © 2019 The Japan Society for Analytical Chemistry. In this work, ionic liquid materials composed of pH indicator dyes were synthesized and used as a plasticizer for a plasticized PVC membrane-based ion-selective optode. We chose several triphenylmethane dyes and synthesized ionic liquids with those by an ion-exchange procedure, and found that an ionic liquid composed of bromothymol blue (BTB) realized the highest ion-exchange ratio of approximately 100% (highest concentration of dye). The absorbance response of the PVC membrane using a BTB-based ionic liquid was decreased by ca. 37% using the repetitive introduction of acid and base solutions, and reached to exhibit a stable and reproducible response. The concentration of dye was 20~70-times higher than that of the conventional ones. In addition, the PVC membrane was applied for anion sensing based on the coextraction of protons and anions in aqueous solutions, and it was found that the ion selectivity followed the Hofmeister series. In the future, the present ionic liquid material is expected to be applied for highly-selective and sensitive ion-selective optodes by the modification of functional groups such as ion-recognition or reaction with the target molecule.

    DOI: 10.2116/bunsekikagaku.68.945

  • ⇜calcium-responsive ionic liquid⇝ for naked eye-based multiplexed ion detection on a pdms microchannel array device

    Mizuta T.

    23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019   1320 - 1321   2019( ISBN:9781733419000

  • An “enzyme-responsive ionic liquid” toward capillary array-based immunoassay microdevices

    Oishi R.

    23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019   1226 - 1227   2019( ISBN:9781733419000

  • Development of a polymer/TiO<inf>2</inf> hybrid two-dimensional photonic crystal for highly sensitive fluorescence-based ion sensing applications

    Shoma Aki, Kenichi Maeno, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Sensors and Actuators, B: Chemical   269   257 - 263   2018.09( ISSN:0925-4005

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    Publishing type:Research paper (scientific journal)  

    © 2018 In this study, we developed a polymer/TiO2 hybrid two-dimensional photonic crystal (2D-PhC) optical ion sensor that shows fluorescence enhancement based on the matching of the fluorescence wavelength and the confinable wavelength of a 2D-PhC. The polymer/TiO2 hybrid PhC was fabricated from a TiO2 pillar array, where the interspace was filled with a polymer containing a fluorescent dye. We developed a method of fabricating the TiO2 pillar array based on nanoimprint lithography using liquid phase deposition. The polymer/TiO2 hybrid PhC was fabricated on a poly(ethylene terephthalate) (PET) film. Thus, it can be easily cut because of its high flexibility and used for ion sensing applications. By using a fluorescent dye-containing polymer (poly(methyl methacrylate), PMMA) for the fabrication of the polymer/TiO2 hybrid PhC, the fluorescence intensity was drastically (25 times) increased. Subsequently, we used an ion-sensing polymer composed of plasticized poly(vinyl chloride) (PVC), a fluorescent dye, and ion recognition elements in the polymer/TiO2 hybrid PhC to enable its use as a highly sensitive ion sensor. As a result, the ion sensor based on the plasticized-PVC/TiO2 hybrid PhC exhibited four times greater fluorescence intensity compared with that based on the plasticized PVC without the PhC structure.

    DOI: 10.1016/j.snb.2018.05.004

  • Design and fabrication of a visible-light-compatible, polymer-based photonic crystal resonator and waveguide for sensing applications

    Jiayi Sun, Kenichi Maeno, Shoma Aki, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Micromachines   9 ( 8 )   2018.08

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    Publishing type:Research paper (scientific journal)  

    © 2018 by the authors. In this paper, we have proposed a polymer-based photonic crystal (PhC) resonator, with multiple sizes of cavities, and a waveguide to be used as highly sensitive optical sensor components. Properties of the proposed PhC were simulated by the finite-difference time-domain method, and the polymer-based PhC resonator and waveguide were fabricated on a photoresist (polymer) by electron beam lithography, which was prepared on an Au-layer-deposited Si substrate. We detected the resonant light that penetrated through the waveguide and was trapped in the PhC resonator. Optical characteristics of the fabricated PhC were evaluated by detecting the polymer layer deposition process by using the layer-by-layer (LbL) method to deposit polymer layers. As a result, by using an optimized design of a polymer-based PhC resonator with a long cavity (equivalent to a defect of three holes), the PhC structure changes caused by LbL deposition lead to changes in resonant light wavelength (peak shift: 5.26 nm/layer). Therefore, we suggest that a PhC resonator and a waveguide is applicable as an optical sensor.

    DOI: 10.3390/mi9080410

  • Ionic liquid-based dye: A “Dyed plasticizer” for rapid and highly sensitive anion optodes based on a plasticized PVC membrane Reviewed

    Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Sens. Actuators B.   258   1125 - 1130   2018

  • Development of a polymer/TiO2 hybrid two-dimensional photonic crystal for highly sensitive fluorescence-based ion sensing applications Reviewed

    Shoma Aki, Kenichi Maeno, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Sens. Actuators B.   269   257 - 263   2018

  • Design and Fabrication of Visible-light-compatible Polymer-based Photonic Crystal Resonator and Waveguide for Sensing Application Reviewed

    Jiayi Sun, Kenichi Maeno, Shoma Aki, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Micromachines   9   2018

  • Regioselective immobilization of PVC membrane composed of ionic liquid-based dye on convex-shaped PDMS surface for multiplexed microanalytical devices Reviewed

    Tatsumi Mizuta, Kenichi Maeno, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Anal. Sci.   34   517 - 519   2018

  • Regioselective immobilization of a PVC membrane composed of an ionic liquid-based dye on convex-shaped PDMS surface for multiplexed microanalytical devices

    Tatsumi Mizuta, Kenichi Maeno, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Analytical Sciences   34 ( 5 )   517 - 519   2018( ISSN:0910-6340

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    © The Japan Society for Analytical Chemistry. A novel method for the intact immobilization of a very-thin and soft PVC membrane on a convex-shaped poly(dimethylsiloxane) (PDMS) surface is described. The present method using PVA film as a sacrificial layer allowed successful immobilization of an intact PVC membrane using an ionic liquid-based dye on only the convex-shaped PDMS surface without any deformation or increase of the inhomogeneity. In addition, two different kinds of PVC membranes were successfully immobilized simultaneously toward multiplexed detection.

    DOI: 10.2116/analsci.18C008

    PubMed

  • Development of Element Technology for 1 STEP Biomarker Protein Analysis Device Using Silver Nanoparticle–Contained Hydrogel and Reagent-Immobilized Cartridge

    Kenzo Yamamoto, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Electronics and Communications in Japan   100 ( 6 )   45 - 53   2017.06( ISSN:1942-9533

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    Publishing type:Research paper (scientific journal)  

    © 2017 Wiley Periodicals, Inc. We fabricated 1 STEP biomarker protein analysis device using silver nanoparticles (AgNPs)-contained hydrogel and reagent-immobilized cartridge. Specific color change of AgNPs based on localized surface plasmon resonance (LSPR) was applied to develop the novel colorimetric protein analysis device. In this report, we constructed this device by four components, AgNPs-contained hydrogel, glucose oxidase (GOx) antibody release pad, glucose release pad, and antibody-immobilized membrane. AgNPs-contained hydrogel detected 0.001 to 10 μg/mL GOx-antibody. AgNPs-contained hydrogel absorbance became altered 90.0% GOx release pad as compared to GOx-antibody solution under the reaction time 60 min. AgNPs-contained hydrogel absorbance became altered 98.4% glucose release pad as compared to glucose solution under the reaction time 60 min. We applied AgNPs-contained hydrogel and antibody-immobilized membrane to detect human immunoglobulin G (IgG). As a result, we found these could detect 1 μg/mL or lower concentration of human IgG.

    DOI: 10.1002/ecj.11960

  • A Simple and Easy-to-Use Capillary Isoelectric Focusing Technique Using Reagent-Release Hydrogels Reviewed

    Yuji FUJII, Kenji SUEYOSHI*, Tatsuro ENDO, Hideaki HISAMOTO

    Chromatography   38   79 - 83   2017

  • Origin of the Optical Response of a Dye-doped Plasticized Poly(vinyl chloride)-based Photonic Crystal Ion Sensor Reviewed

    Shoma Aki, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Anal. Sci.   33   1247 - 1251   2017

  • Fast and Single-step Fluorescence-based Competitive Bioassay Microdevice Using Combined PDMS Microchannel Arrays Separately Immobilizing Graphene Oxide-Analyte Conjugates and Fluorescently-labelled Receptor Proteins Reviewed

    Akihiro Shirai, Kaho Nakashima, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Anal. Sci.   33   969 - 972   2017

  • Double sweeping: Highly effective sample preconcentration using cationic and anionic micelles and its application to a multiple enzyme activity assay Reviewed

    Ryota Sanuki, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Anal. Chem.   89   6505 - 6512   2017

  • Development of a single-step immunoassay microdevice based on a graphene oxide-containing hydrogel possessing fluorescence quenching and size separation functions Reviewed

    Akihiro Shirai, Kaho Nakashima, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Analyst   142   472 - 477   2017

  • Development of cartridge-based wash-free single-step plasmonic enzyme-linked immunosorbent assay using poly(vinylpyrrolidinone)-coated silver nanoparticles as a chromogenic substrate

    Tatsuro Endo, Keiizo Yamamoto, Kenji Sueyoshi, Hideaki Hisamoto

    Sensors and Materials   29 ( 9 )   1247 - 1252   2017( ISSN:0914-4935

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    In this study, polyvinylpyrrolidone) (PVP)-coated silver nanoparticles were used as a chromogenic substrate for the establishment of a colorimetric enzyme-linked immunosorbent assay (ELISA) based 011 localized surface plasmon resonance (LSPR) called "plasmonic ELISA". The PVP-coated silver nanoparticles exhibit a specific color due to the LSPR. In addition, the LSPR absorbance strength of the PVP-coated silver nanoparticles is changed by the aggregation of the silver nanoparticles which depends 011 the hydrogen peroxide concentration. From these changes in the LSPR absorbance strength in relation to hydrogen peroxide concentration, plasmonic ELISA was established using a glucose oxidase (GOx)-conjugated secondary antibody and glucose for producing hydrogen peroxide by an enzymatic reaction. Furthermore. 011 the basis of plasmonic ELISA. a cartridge-based single-step plasmonic ELISA device was fabricated for the realization of a wash-free ELISA system. This cartridge-based ELISA system is constructed with a GOx-Antibody (GOx-conjugated secondary antibody) release pad. a primary antibody immobilized membrane, a glucose release pad, and a PVP-coated silver nanoparticle-containing hydrogel. By using this construction, the antigen concentration can be determined by introducing only a sample solution. As a result, with this cartridge-based ELISA system, the antigen-Antibody reaction was successfully detected at different concentrations of antigen (0.1-10 μg/ml).

    DOI: 10.18494/SAM.2017.1642

  • Development of optical biosensor based on photonic crystal made of TiO

    Aono Keigo, Aki Shoma, Sueyoshi Kenji, Hisamoto Hideaki, Endo Tatsuro

    Jpn. J. Appl. Phys.   55 ( 8 )   08RE01   2016.07( ISSN:0021-4922

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    We fabricated a titanium dioxide (TiO<inf>2</inf>)-based photonic crystal (PhC) using liquid phase deposition (LPD) to develop highly sensitive optical biosensors. The optical characteristics of the PhCs in the visible region were sensitive to the change in the refractive index of the surrounding medium due to an antigen–antibody reaction; thus, applications using the optical biosensor are expected to be highly sensitive. However, a base material with a high refractive index is indispensable for the fabrication of the PhC. Here, TiO<inf>2</inf>, which has optical transparency in the visible region, was selected as the high refractive index base material. The present LPD method allowed fabrication using low-cost apparatus. Furthermore, the mild conditions of the LPD method led to formation of TiO<inf>2</inf>-based PhC with fewer crack structures. Finally, the anti-neuron-specific enolase antibody was immobilized onto the TiO<inf>2</inf>-based PhC surface, and 1–1000 ng/mL of the neuron-specific enolase antigen was successfully detected.

    DOI: 10.7567/JJAP.55.08RE01

    CiNii Article

  • Fabrication of gold-deposited plasmonic crystal based on nanoimprint lithography for label-free biosensing application Reviewed

    Kiichi Nishiguchi, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Jpn. J. Appl. Phys.   55   2016

  • Development of optical biosensor based on photonic crystal made of TiO2using liquid phase deposition Reviewed

    Keigo Aono, Shoma Aki, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Jpn. J. Appl. Phys.   55   2016

  • Fast and single-step immunoassay based on fluorescence quenching within a square glass capillary immobilizing graphene oxide-antibody conjugate and fluorescently labelled antibody Reviewed

    Akihiro Shirai, Terence Henares, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Analyst   141   3389 - 3394   2016

  • A Simple and Rapid Immunoassay Based on Microchip Electrophoresis Using a Reagent-Release Cartridge Reviewed

    Kenji Sueyoshi, Yuta Miyahara, Tatsuro Endo, Hideaki Hisamoto

    Chromatography   37   29 - 33   2016

  • Polymer-based Photonic Crystal Cavity Sensor for Optical Detection in the Visible Wavelength Region Reviewed

    Kenichi Maeno, Shoma Aki, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    Anal. Sci.   32   117 - 120   2016

  • A single-step enzyme immunoassay capillary sensor composed of functional multilayer coatings for the diagnosis of marker proteins

    Shun Ichi Funano, Masato Sugahara, Terence G. Henares, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Analyst   140 ( 5 )   1459 - 1465   2015.03( ISSN:0003-2654

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    © The Royal Society of Chemistry. A single-step, easy-to-use enzyme immunoassay capillary sensor, composed of functional multilayer coatings, was developed in this study. The coatings were composed of substrate-immobilized hydrophobic coating, hydrogel coating, and soluble coating containing an enzyme-labeled antibody. The response mechanism involved a spontaneous immunoreaction triggered by capillary action-mediated introduction of a sample antigen solution and subsequent separation of unreacted enzyme-labeled antibodies and antigen-enzyme-labeled antibody complexes by the molecular sieving effect of the hydrogel. An enzyme reaction at the substrate-immobilized hydrophobic coating/hydrogel coating interface resulted in a protein-selective fluorescence response. An antigen concentration-dependent response was obtained for diagnostic marker protein samples (hemoglobin A1c (HbA1c), 7.14-16.7 mg mL-1; alpha-fetoprotein (AFP), 1.4-140 ng mL-1; C-reactive protein (CRP), 0.5-10 μg mL-1) that cover a clinically important concentration range. The successful measurement of CRP in diluted serum samples demonstrated the application of this capillary sensor. This journal is

    DOI: 10.1039/c4an01781a

    PubMed

  • Highly Sensitive and Multiple Enzyme Activity Assay Using Reagent-release Capillary-Isoelectric Focusing with Rhodamine 110-based Substrates Reviewed

    Kenji Sueyoshi, Yuto Nogawa, Kasumi Sugawara, Tatsuro Endo, Hideaki Hisamoto

    Anal. Sci.   31   1155 - 1161   2015

  • Development of element technology for 1 STEP biomarker protein analysis device using silver nanoparticle contained hydrogel and reagent immobilized cartridge

    Kenzo Yamamoto, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    IEEJ Transactions on Electronics, Information and Systems   135   1307 - 1313   2015

  • Development of Microchip Electrophoresis-Integrated Nanoimprinted Photonic Crystal Reviewed

    Tatsuro Endo, Kenzo Yamamoto, Kenji Sueyoshi, Hideaki Hisamoto

    Sens. Mater.   27   425 - 433   2015

  • Fabrication and packaging of a mass-producible capillary-assembled microchip for simple and multiplexed bioassay Reviewed

    Terence Henares, Akihiro Shirai, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Sens. Actuators B   218   245 - 252   2015

  • Separation Behavior of Short Oligonucleotides by Ion-Pair Reversed-Phase Capillary Liquid Chromatography Using a Silica-Based Monolithic Column Applied to Simple Detection of SNPs Reviewed

    Kunio Kawamura, Keisuke Ikoma, Yoshimi Maruoka, Hideaki Hisamoto

    Chromatographia   78   487 - 494   2015

  • A single-step enzyme immunoassay capillary sensor composed of functional multilayer coatings for diagnosis marker proteins Reviewed

    Shun-ichi Funano, Masato Sugahara, Terence Henares, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Analyst   140   1459 - 1465   2015

  • Efficient Immobilization of Enzyme and Substrate for Single-Step Caspase-3 Inhibitor Assay Using Combinable PDMS Capillary Sensor Array Reviewed

    Tadashi Ishimoto, Kaede Jigawa, Terence Henares, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    RSC Adv.   4   47682 - 7687   2014

  • Plasticized poly(vinyl chloride)-based photonic crystal for ion sensing Reviewed

    Shoma Aki, Tatsuro Endo, Kenji Sueyoshi, Hideaki Hisamoto

    Anal. Chem.   86   11986 - 11991   2014

  • Development of novel protease assay device using a nanoimprinted two dimensional photonic crystal Reviewed

    Wakana Hashimoto, Tatsuro Endo, Kenji Sueyoshi, Hideaki Hisamoto

    Chem. Lett.   43   1728 - 1730   2014

  • Signal amplified two-dimensional photonic crystal biosensor immobilized with glyco-nanoparticles

    Yuhei Terada, Wakana Hashimoto, Tatsuro Endo, Hirokazu Seto, Tatsuya Murakami, Hideaki Hisamoto, Yu Hoshino, Yoshiko Miura

    J. Mater. Chem. B   2   3324 - 3332   2014

  • Efficient immobilization of the enzyme and substrate for a single-step caspase-3 inhibitor assay using a combinable PDMS capillary sensor array

    Tadashi Ishimoto, Kaede Jigawa, Terence G. Henares, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    RSC Advances   4 ( 15 )   7682 - 7687   2014

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    The caspase-3 inhibitor assay, which is important for drug development was successfully integrated into a "single step" by solving the immobilization problem of enzymes with low activity. Here we used the soluble polyethylene glycol (PEG) coatings for enzyme immobilization to the concave-shaped PDMS surface, and substrate immobilization to the convex-shaped PDMS surface. Then these PDMS structures were combined to form a capillary-structure, which we call a combinable poly (dimethylsiloxane) (PDMS) capillary (CPC) sensor, enabling simultaneous immobilization of two different reactive reagents such as enzymes and fluorescent substrates. The present sensor was disposable, and the fluorescence response was simply obtained by introducing the sample solution by capillary action. The caspase-3 activity was able to be maintained at approximately 90% under -80 °C storage conditions even after 5 months. Importantly, the total reaction time was reduced from an hour to 3-5 min, and simultaneous acquisition of multiple data sets required to determine IC50 value was also successfully achieved using the CPC sensor array. © 2014 The Royal Society of Chemistry.

    DOI: 10.1039/c3ra46976j

  • Design of a single-step immunoassay principle based on the combination of an enzyme-labeled antibody release coating and a hydrogel copolymerized with a fluorescent enzyme substrate in a microfluidic capillary device

    Hideki Wakayama, Terence G. Henares, Kaede Jigawa, Shun Ichi Funano, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Lab on a Chip   13 ( 22 )   4304 - 4307   2013.11( ISSN:1473-0197

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    A combination of an enzyme-labeled antibody release coating and a novel fluorescent enzyme substrate-copolymerized hydrogel in a microchannel for a single-step, no-wash microfluidic immunoassay is demonstrated. This hydrogel discriminates the free enzyme-conjugated antibody from an antigen-enzyme- conjugated antibody immunocomplex based on the difference in molecular size. A selective and sensitive immunoassay, with 10-1000 ng mL-1 linear range, is reported. © 2013 The Royal Society of Chemistry.

    DOI: 10.1039/c3lc50775k

    PubMed

  • Capillary-based enzyme-linked immunosorbent assay for highly sensitive detection of thrombin-cleaved osteopontin in plasma

    Shun-ichi Funano, Terence Henares, Mie Kurata, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Anal. Biochem   440   137 - 141   2013.09

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  • Enhancement of the fluorescence intensity of DNA intercalators using nano-imprinted 2-dimensional photonic crystal

    Tatsuro Endo, China Ueda, Hiroshi Kajita, Norimichi Okuda, Satoru Tanaka, Hideaki Hisamoto

    Microchim. Acta   180   929 - 934   2013.06

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  • Novel fluorescent probe for highly sensitive bioassay using sequential enzyme-linked immunosorbent assay-capillary isoelectric focusing (ELISA-cIEF)

    Terence Henares, Yuta Uenoyama, Yuto Nogawa, Ken Ikegami, Daniel Citterio, Koji Suzuki, Shun-ichi Funano, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Analyst   138   3139 - 3141   2013.04

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  • Integration of neuraminidase inhibitor assay into a single-step operation using a combinable poly(dimethylsiloxane) capillary sensor

    Tadashi Ishimoto, Kaede Jigawa, Terence Henares, Tatsuro Endo, Hideaki Hisamoto

    Analyst   138   3158 - 3162   2013.03

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  • Design of Single-Step Immunoassay Principle Based on the Combination of Enzyme-Labeled Antibody Release Coating and Hydrogel Copolymerized with Fluorescent Enzyme Substrate in a Microfluidic Capillary Device

    Hideki Wakayama, Terence Henares, Kaede Jigawa, Shun-ichi Funano, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Lab Chip   13   4304 - 4307   2013

  • Open-Type Capillary-Assembled Microchip for Rapid, Single-Step, Simultaneous Multi-Component Analysis of Serum Sample

    Yusuke Kimura, Terence Henares, Shun-ichi Funano, Tatsuro Endo, Hideaki Hisamoto

    RSC Adv   2   9525 - 9530   2012.08

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  • Bulk- and surface-modified combinable PDMS capillary sensor array as an easy-to-use sensing device with enhanced sensitivity to elevated concentrations of multiple serum sample components

    Yuji Fujii, Terence G. Henares, Kunio Kawamura, Tatsuro Endo, Hideaki Hisamoto

    Lab on a Chip   12 ( 8 )   1522 - 1526   2012.04( ISSN:1473-0197

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    Publishing type:Research paper (scientific journal)  

    To enhance sensitivity and facilitate easy sample introduction into a combinable poly(dimethylsiloxane) (PDMS) capillary (CPC) sensor array, PDMS was modified in bulk and on its surface to prepare "black" PDMS coated with a silver layer and self-assembled monolayer (SAM). India ink, a traditional Japanese black ink, was added to the PDMS pre-polymer for bulk modification. The surface was modified by a silver mirror reaction followed by SAM formation using cysteine. These modifications enhanced the fluorescence signals by reflecting them from the surface and reducing background interference. A decrease in the water contact angle led to enhanced sensitivity and easy sample introduction. Furthermore, a CPC sensor array for multiplex detection of serum sample components was prepared that could quantify the analytes glucose, potassium, and alkaline phosphatase (ALP). When serum samples were introduced by capillary action, the CPC sensor array showed fluorescence responses for each analyte and successfully identified the components with elevated concentrations in the serum samples. © The Royal Society of Chemistry.

    DOI: 10.1039/c2lc21242k

  • Combinable Poly(Dimethyl siloxane) Capillary Sensor Array for Single-Step and Multiple Enzyme Inhibitor Assays

    Yosuke Uchiyama, Fumito Okubo, Kozo Akai, Yuji Fujii, Terence G. Henares, Kunio Kawamura, Toshio Yao, Tatsuro Endo, Hideaki Hisamoto

    Lab Chip   12   204 - 208   2012

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  • DESIGN AND SYNTHESIS OF FLUORESCENT ENZYME SUBSTRATE MONOMER AND ITS APPLICATION TO THE DEVELOPMENT OF HYDROGEL-BASED SINGLE STEP IMMUNOASSAY MICRODEVICE

    Hideki Wakayama, Seiji Odaka, Shun-ichi Funano, Terence Henares, Tatsuro Endo, Hideaki Hisamoto

    Proc. MicroTAS 2012   806 - 808   2012

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  • CAPILLARY SENSOR ARRAY CHIP AS A “SAMPLE-TO-ANSWER” DEVICE FOR SIMPLE, RAPID, AND MULTIPLE COMPONENT ANALYSIS OF SERUM SAMPLE

    Yusuke Kimura, Terence Henares, Shun-ichi Funano, Tatsuro Endo, Hideaki Hisamoto

    Proc. MicroTAS 2012   1384 - 1386   2012

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  • DEVELOPMENT OF LABEL-FREE BIOSENSOR FOR THE DETECTION OF ADENOSINE DIPHOSPHATE AS A UNIVERSAL KINASE/ATPASE ASSAY USING NANOIMPRINTED FLEXIBLE TWO-DIMENSIONAL PHOTONIC CRYSTAL

    Tatsuro Endo, Bernadette Henares, Hideaki Hisamoto

    Proc. MicroTAS 2012   950 - 952   2012

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  • Printed two-dimensional photonic crystals for single-step label-free biosensing of insulin under wet conditions

    Tatsuro Endo, Masaya Sato, Hiroshi Kajita, Norimichi Okuda, Satoru Tanaka, Hideaki Hisamoto

    Lab Chip   12   1995 - 1999   2012

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  • Bulk- and Surface-Modified Combinable PDMS Capillary Sensor Array as Easy-to-Use Sensing Device with Enhanced Sensitivity to Elevated Concentrations of Multiple Serum Sample Components

    Yuji Fujii, Terence G. Henares, Kunio Kawamura, Tatsuro Endo, Hideaki Hisamoto

    Lab Chip   12   1522 - 1526   2012

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  • Reagent-Release Capillary Array-Isoelectric Focusing Device as a Rapid Screening Device for IEF Condition Optimization Reviewed

    M.Kataoka, H.Yokoyama, T.G.Henares, K.Kawamura, T.Yao, and H. Hisamoto

    Lab Chip.   10 ( 24 )   3341 - 3347   2010.08

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  • Single-step ELISA Capillary Sensor Based on Surface-Bonded Glucose Oxidase, Antibody, and Physically-Adsorbed PEG membrane Containing Peroxidase-Labeled Antibody Reviewed

    M.Kataoka, H.Yokoyama, T.G.Henares, K.Kawamura, T.Yao, and H. Hisamoto

    Sens.Actuators B.   149 ( 1 )   319 - 324   2010.06

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  • Facile Preparation Method of a Disposable Capillary Biosensor Using an Ion-selective Optode Membrane and a Dissolvable Enzyme Membrane and Its Application to Urea Sensing Reviewed

    E.Tsutsumi, T.G.Henares, K.Kawamura, T.Yao, and H.Hisamoto

    Chem. Lett.   39   436 - 438   2010.03

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  • Facile preparation method of a disposable capillary biosensor using an ion-selective optode membrane and a dissolvable enzyme membrane and its application to urea

    Erina Tsutsumi, Terence G. Henares, Kunio Kawamura, Toshio Yao, Hideaki Hisamoto

    Chemistry Letters   39 ( 5 )   436 - 438   2010( ISSN:0366-7022

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    Publishing type:Research paper (scientific journal)  

    A facile preparation method of a disposable capillary biosensor immobilizing a plasticized poly(vinyl chloride) (PVC) membrane ion-selective optode containing a lipophilic pH indicator dye and an ionophore, and a dissolvable poly(ethylene glycol) (PEG) membrane containing an enzyme is developed. The method involves the sequential introduction of membrane cocktail solution followed by air introduction. Repeating this process allowed facile preparation of a capillary biosensor comprised of two layer structure, which can be used for single-step biosensing just by sipping the sample with capillary action. Here a urea biosensor was prepared as a proof-of-concept. © 2010 The Chemical Society of Japan.

    DOI: 10.1246/cl.2010.436

  • Enzyme-release capillary as a facile enzymatic biosensing part for a capillary-assembled microchip

    Terence G. Henares, Eiji Maekawa, Fumito Okubo, Fumio Mizutani, Toshio Yao, Ryuichi Sekizawa, Hideaki Hisamoto

    Analytical Sciences   25 ( 8 )   1025 - 1028   2009.08( ISSN:0910-6340

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    A simple capillary enzymatic biosensor was developed. This was prepared by simply coating a dissolvable membrane containing enzyme/s on the inner wall of a square glass capillary. An easy measurement was carried out by capillary force sample introduction with concurrent enzyme release and a reaction with a certain substrate. Enzyme-release capillary (ERC) biosensors showed long-term storage stability of at least two weeks for a β-galactoside derivative and glucose. Moreover, this could be integrated on a capillary-assembled microchip (CAs-CHIP) to broaden its multiple analyte sensing potential for clinical diagnostic applications. 2009 © The Japan Society for Analytical Chemistry.

    DOI: 10.2116/analsci.25.1025

    PubMed

  • Single-drop analysis of various proteases in a cancer cell lysate using a capillary-assembled microchip

    Terence G. Henares, Fumio Mizutani, Ryuichi Sekizawa, Hideaki Hisamoto

    Analytical and Bioanalytical Chemistry   391 ( 7 )   2507 - 2512   2008.08( ISSN:1618-2642

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    Publishing type:Research paper (scientific journal)  

    Single-drop analysis of two different real sample solutions (2 μL) while simultaneously monitoring the activity of two sets of ten different proteases on a single microfluidic device is presented. The device, called a capillary-assembled microchip (CAs-CHIP), is fabricated by embedding square glass sensing capillaries (reagent-release capillaries, RRC) in the polydimethylsiloxane (PDMS) lattice microchannel, and used for that purpose. First, the performance reliability was evaluated by measuring the fluorescence response of twenty caspase-3-sensing capillaries on a single CAs-CHIP, and a relative standard deviation of 1.5-8.2 (% RSD, n∈=∈5 or 10) was obtained. This suggests that precise multiplexed protease-activity sensing is possible by using a single CAs-CHIP with multiple RRCs embedded. Then, using a single CAs-CHIP, real sample analysis of the activity of ten different caspases/proteases in cervical cancer (HeLa) cell lysate treated and untreated with the cell-death-inducer drug, doxorubicin, was simultaneously carried out, and a significant difference in enzyme activity between these two samples was observed. These results suggested the usefulness of the CAs-CHIP in the field of drug discovery. © 2008 Springer-Verlag.

    DOI: 10.1007/s00216-008-2105-x

    PubMed

  • "Drop-and-sip" fluid handling technique for the reagent-release capillary (RRC)-based capillary-assembled microchip (CAs-CHIP): Sample delivery optimization and reagent release behavior in RRC

    Terence G. Henares, Fumio Mizutani, Ryuichi Sekizawa, Hideaki Hisamoto

    Analytical Sciences   24 ( 1 )   127 - 132   2008.01( ISSN:0910-6340

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    The experimental conditions of the sample delivery inside the reagent-release capillary-based capillary-assembled microchip (RRC-based CAs-CHIP) were optimized and the reagent release procedure in the RRC is discussed. Recently, our group introduced the basic concept of the "drop-and-sip" fluid handling technique (Anal. Chem., 2007, 79, 908). A microliter volume of sample solution is dropped on the inlet hole and is sipped into another hole, producing a sample plug flow in the main poly(dimethyl siloxane) (PDMS) channel, concurrently filling each sensing capillary that faces the main PDMS channel. However, the detailed evaluation of the successful sample delivery condition and the reagent release behavior in the RRC has not been fully discussed. Under our experimental conditions, ca. 0.6 - 2.4 s of sample plug-RRC contact time allowed the successful sample introduction into the RRC by capillary force without any reagent leakage or disturbance of the sample plug flow. On the other hand, reagent release behavior inside the RRC is governed by both convective and diffusive mass transport, which leads to a faster mixing time of the sample with reagents immobilized inside the RRC compared to that expected from the simple diffusion alone. 2008 © The Japan Society for Analytical Chemistry.

    DOI: 10.2116/analsci.24.127

    PubMed

  • Drop-and-Sip Fluid Handling Technique for the Reagent-Release Capillary (RRC)-based Capillary-Assembled Microchip (CAs-CHIP): Sample Delivery Optimization and Reagent Release Behavior in RRC Reviewed

    erence G. HenaresD, Fumio Mizutani, Ryuichi Sekizawa, and Hideaki Hisamoto

    Anal. Sci. 雑誌   24   127 - 132   2008

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  • Enzyme-Release Capillary as a Facile Enzymatic Biosensing Parts for Capillary-Assembled Microchip Reviewed

    Terence G. Henares, Eiji Maekawa, Fumito Okubo, Fumio Mizutani, Ryuichi Sekizawa, Toshio Yao, and Hideaki Hisamoto

    Anal. Sci. 雑誌   1025 - 1028   2008

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  • Single Drop Analysis of Various Proteases in a Cancer Cell Lysate using Capillary-Assembled Microchip Reviewed

    Terence G. HenaresD, Fumio Mizutani, Ryuichi Sekizawa, and Hideaki Hisamoto

    Anal. Bioanal. Chem. 雑誌   391   2507 - 2512   2008

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  • Integration of multianalyte sensing functions on a capillary-assembled microchip: Simultaneous determination of ion concentrations and enzymatic activities by a "drop-and-sip" technique

    Terence G. Henares, Masayuki Takaishi, Naoya Yoshida, Shigeru Terabe, Fumio Mizutani, Ryuichi Sekizawa, Hideaki Hisamoto

    Analytical Chemistry   79 ( 3 )   908 - 915   2007.02( ISSN:0003-2700

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    Publishing type:Research paper (scientific journal)  

    A general and simple implementation of simultaneous multiparametric sensing in a single microchip is presented by using a capillary-assembled microchip (CAs-CHIP) integrated with the plural different reagent-release capillaries (RRCs), acting as various biochemical sensors. A novel "drop-and-sip" technique of fluid handling is performed with a microliter droplet of a model sample solution containing proteases (trypsin, chymotrypsin, thrombin, elastase) and divalent cations (Ca2+, Zn2+, Mg2+) that passes through the microchannel with the aid of a micropipette as a vacuum pump, concurrently filling each RRC via capillary force. To avert the evaporation of the nanoliter sample volume in each capillary, PDMS oil is dropped on the outlet hole of the CAs-CHIP exploiting the capillary force that results in spontaneous sealing of all the RRCs. In addition, this high-speed sample introduction alleviates the possibility of protein adsorption and capillary cross-contamination, allowing a reliable and multianalyte determination of a sample containing many different proteases and divalent cations by using the fluorescence image analysis. Presented results suggested the possible application of this microchip in the field of drug discovery and systems biology. © 2007 American Chemical Society.

    DOI: 10.1021/ac061245i

    PubMed

  • pH-Independent Fluorescent Chemosensor for Highly Selective Lithium Ion Sensing Reviewed

    Daniel Citterio, Junichiro Takeda, Masaki Kosugi, Hideaki Hisamoto, Shin-ichi Sasaki, Hirokazu Komatsu and Koji Suzuki

    Anal.Chem. 雑誌   79 ( 3 )   1237 - 1242   2007.02

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  • Characterization of Electrokinetic Gating Valve in Microfluidic Channels Reviewed

    Guiseng Zhang, Wei Du, Bi-Feng Liu, Hideaki Hisamoto and Shigeru Terabe

    Anal. Chim. Acta 雑誌   584 ( 1 )   129 - 135   2007

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  • Capillary-assembled Microchip for the Electrochemical Determination of Glucose Reviewed

    Shingo Inadumi, Hideaki Hisamoto, and Fumio Mizutani

    Chem.Lett. 雑誌   36 ( 10 )   1242 - 1243   2007

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  • Characterization of Capillary Immunosensor for the Capillary-Assembled Microchip (CAs-CHIP) Integration Reviewed

    Terence G. Henares, Fumio Mizutani, Ryuichi Sekizawa and Hideaki Hisamoto

    Sens.Mater. 雑誌   19 ( 4 )   249 - 259   2007

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  • Multiple Enzyme Linked Immunosorbent Assay System on a Capillary-Assembled Microchip Integrating Valving and Immuno-Reaction Functions Reviewed

    Terence G. HenaresD, Shun-ichi Funano, Shigeru Terabe, Fumio Mizutani, Ryuichi Sekizawa and Hideaki Hisamoto

    Anal. Chim. Acta 雑誌   589 ( 2 )   173 - 179   2007

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  • Integration of Multi-Analyte Sensing Functions on a Capillary-Assembled Microchip: Simultaneous Determination of Ion Concentration and Enzymatic Activities by "Drop-and-Sip" Technique Reviewed

    Terence G. HenaresD, Masayuki Takaishi, Naoya Yoshida, Shigeru Terabe, Fumio Mizutani, Ryuichi Sekizawa and Hideaki Hisamoto

    Anal.Chem. 雑誌   79 ( 3 )   908 - 915   2007

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  • Characterization of capillary immunosensor for capillary-assembled microchip (CAs-CHIP) integration

    Terence G. Henares, Fumio Mizutani, Ryuichi Sekizawa, Hideaki Hisamoto

    Sensors and Materials   19 ( 4 )   249 - 259   2007( ISSN:0914-4935

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    A capillary immunosensor is developed as a sensing unit of a capillary-assembled microchip (CAs-CHIP) that is geared towards simultaneous multiple immunoassay. It is prepared by subsequently treating the inner surface of a square glass capillary (internal dimension 50 μm) with 3-aminopropyltriethoxysilane -glutaraldehyde-protein A (APTES-GA-Protein A), which significantly reduced the non-specific adsorption. Consequently, accurate quantitative immunoassay measurement is demonstrated using human and chicken IgG as model samples, yielding a detection limit of about 1 ng mL-1 for both antigens with a total analysis time of about 60 min. The immunosensor also displayed interesting practical properties like long-term stability of at least a month at 10°C and minimal consumption of secondary labeled-antibody with one-million-fold dilution. It is expected that the integration of this fluorescent immunosensing unit into the CAsCHIP will enhance the analytical performance of the microchip through simultaneous multiple immunoassay in a single microfluidic device. This type of biochip may have a significant impact in clinical diagnostics and drug-screening applications.

  • Capillary-assembled microchip as an on-line deproteinization device for capillary electrophoresis

    Hideaki Hisamoto, Seigi Takeda, Shigeru Terabe

    Analytical and Bioanalytical Chemistry   386 ( 3 )   733 - 738   2006.10( ISSN:1618-2642

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    A capillary-assembled microchip (CAs-CHIP), prepared by simply embedding square capillaries in a lattice polydimethylsiloxane (PDMS) channel plate with the same channel dimensions as the outer dimensions of the square capillaries, has been used as a diffusion-based pretreatment attachment in capillary electrophoresis (CE). Because the CAs-CHIPs employ square-section channels, diffusion-based separation of small molecules from sample solutions containing proteins is possible by using the multilayer flow formed in the square section channel. When a solution containing high-molecular-weight and low-molecular-weight species makes contact with a buffer solution, the low-molecular-weight species, which have larger diffusion coefficients than the high-molecular-weight species, can be collected in a buffer-solution phase. The collected solution containing the low-molecular-weight species is introduced into the separation capillary to be analyzed by CE. This type of system can be used for CE analysis in which pretreatment is required to remove proteins. In this work a fluorescently labeled protein and rhodamine-based molecules were chosen as model species and a feasibility study was performed. © Springer-Verlag 2006.

    DOI: 10.1007/s00216-006-0331-7

  • Healthcare Chip Based on Integrated Electrochemical Sensors Used for Clinical Diagnostics of Bun Reviewed

    Chia-Hsien Chang, Hiroki Ogawa, Akio Oki, Madoka Takai, Masao Nagai, Hideaki Hisamoto and Yasuhiro Horiike

    Jpn. J. Appl. Phys. 雑誌   45 ( 5A )   4241 - 4247   2006.05

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  • Measurement of monomolecular binding constants of neutral phenols into the β-cyclodextrin by continuous frontal analysis in capillary and microchip electrophoresis via a competitive assay

    Thomas Le Saux, Hideaki Hisamoto, Shigeru Terabe

    Journal of Chromatography A   1104 ( 1-2 )   352 - 358   2006.02( ISSN:0021-9673

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    Measurement of binding constant by chip electrophoresis is a very promising technique for the high throughput screening of non-covalent interactions. Among the different electrophoretic methods available that yield the binding parameters, continuous frontal analysis is the most appropriate for a transposition from capillary electrophoresis (CE) to microchip electrophoresis. Implementation of this methodology in microchip was exemplified by the measurement of inclusion constants of 2-naphtalenesulfonate and neutral phenols (phenol, 4-chlorophenol and 4-nitrophenol) into β-cyclodextrin by competitive assays. The issue of competitor choice is discussed in relation to its appropriateness for proper monitoring of the interaction. © 2005 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.chroma.2005.11.125

    PubMed

  • Determination of Monomolecular Binding Constants by Continuous Frontal Analysis in Capillary and Microchip Electrophoresis: Application to Measurement of Neutral Inclusion Constants of Neutral Phenols into the ?-Cyclodextrin via a Competitive Assay Reviewed

    Thomas LeSaux, Hideaki Hisamoto, and Shigeru Terabe

    J. Chromatogr. A 雑誌   1104 ( 1-2 )   352 - 358   2006.02

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  • Integration of Multiple-Ion Sensing on a Capillary-Assembled Microchip Reviewed

    Hideaki Hisamoto, Midori Yasuoka, and Shigeru Terabe

    Anal. Chim. Acta 雑誌   556 ( 1 )   164 - 170   2006.01

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  • Capillary-Assembled Microchip as a Deproteinization Device for Capillary Electrophoresis Separation Reviewed

    Hideaki Hisamoto, Seigi Takeda, and Shigeru Terabe

    Anal. Bioanal. Chem.. 雑誌   2006

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  • Continuous-flow chemical processing in three-dimensional microchannel network for on-chip integration of multiple reactions in a combinatorial mode

    Yoshikuni Kikutani, Masaharu Ueno, Hideaki Hisamoto, Manabu Tokeshi, Takehiko Kitamori

    QSAR and Combinatorial Science   24 ( 6 )   742 - 757   2005.08( ISSN:1611-020X

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    Chemical syntheses using microchemical chips have been intensively investigated in recent years, but they have still not fully reached the stage of practical use. Combinatorial synthesis has been expected to be one of their useful applications. Benefits of using microchips, such as reduction in quantity of reagents and wastes, are thought to be suited for combinatorial syntheses, where numerous variations of products have to be synthesized each in small amounts. There are two modes of combinatorial syntheses using microchemical chips. One is sequential synthesis, in which reactant solutions are introduced sequentially in various combinations to a reaction microchannel. The other is parallel synthesis, where the number of reaction channels is the same as the number of final products, and starting materials are distributed to all the reaction channels in different combinations. Sequential synthesis has been considered as the more favorable way, because, especially when libraries of starting materials are large, numerous microchannels and complicated pipings for distribution of reagents to the channels are required for the parallel mode. Therefore, research on parallel microcombinatorial syntheses has been done only rarely, whereas there have been many reports of sequential microcombinatorial syntheses. Against this trend, our research group has taken an alternative path and tried to integrate both parallel reaction microchannels onto a glass microchip and channels for distributing reactant solutions to each reaction channel in a combinatorial mode. Microchips fabricated by laminating multiple layers of glass substrates have been used to construct three-dimensional microchannel networks necessary for mixing reagent flows in different combinations. We utilized low Reynolds number multi-phase laminar flow and developed micro-unit operations (MUOs). By combining MUOs in the multi-phase continuous laminar flows, microchemical systems were constructed. We called this micro-integration methodology "continuous-flow chemical processing (CFCP)", and by using it, a sequence of various operations in chemical syntheses could be integrated on a microchemical chip. Not just simple mixing of two reagents, which was most commonly done in microreactors, but also multi-step processes including other chemical operations, such as extraction and separation, could be carried out via CFCP. In the first part of this short review article, we explain CFCP with an example. Then, a microchemical chip with a three-dimensional microchannel network for a parallel reaction system is introduced. Finally, we present a case in which CFCP was realized in a three-dimensional microchannel network. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

    DOI: 10.1002/qsar.200540002

  • Integration of Valving and Sensing on a Capillary-Assembled Microchip Reviewed

    Hideaki Hisamoto, Shun-ichi Funano, and Shigeru Terabe

    Anal. Chem. 雑誌   77 ( 7 )   2266 - 2271   2005.04

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  • Development of novel chemical sensing systems based on microfluidic devices

    Hideaki Hisamoto

    Bunseki Kagaku   54 ( 4 )   267 - 278   2005.04( ISSN:0525-1931

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    A recent microfabrication technology opened the new application field such as micro total analysis systems (Micro TAS) or microreactors. These systems employed physical characteristics provided by the liquid microspace, involving short diffusion distance, large surface to volume ratio, or small heat capacity. In contrast to these works, we focused on the chemical functions of highly-selective molecular recognition molecules or functional dyes of which we have been developed, to be integrated into microfluidic chemical sensing systems. The present paper describes our recent results concerning chemical sensing systems based on microfluidic devices. Here, a multi ion sensing system based on the intermittent reagent pumping and ion-pair extraction, a new biosensing scheme based on the enzyme-immobilized polymer membrane, and a novel multi ion sensing scheme based on capillary-assembled microchip, are presented. © 2005 The Japan Society for Analytical Chemistry.

    DOI: 10.2116/bunsekikagaku.54.267

  • Microfluidic Chip toward Cellular ATP and ATP-Conjugated Metabolic Analysis with Bioluminescence Detection Reviewed

    Bi-Feng Liu, Motoaki Ozaki, Hideaki Hisamoto, Qingming Luo, Yuichi Utsumi, Tadashi Hattori, and Shigeru Terabe

    Anal. Chem. 雑誌   77 ( 2 )   573 - 578   2005.01

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  • Capillary-Assembled Microchip for Universal Integration of Various Chemical Functions onto a Single Microfluidic Device Reviewed

    Hideaki Hisamoto, Yuya Nakashima, Chihiro Kitamura, Shun-ichi Funano, Midori Yasuoka, Keisuke Morishima, Yoshikuni Kikutani, Takehiko Kitamori and Shigeru Terabe

    Anal. Chem. 雑誌   76 ( 11 )   3222 - 3228   2004.06

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  • Characterization of molecular transport in poly(dimethylsiloxane) microchannels for electrophoresis fabricated with synchrotron radiation-lithography and UV-photolithography Reviewed

    Bi-Feng Liu, Motoaki Ozaki, Hideaki Hisamoto, Yuichi Utsumi, Tadashi Hattori and Shigeru Terabe

    Lab on a Chip, 雑誌   4 ( 4 )   368 - 371   2004.03

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  • Subsecond Separation of Cellular Flavin Coenzymes by Microchip Capillary Electrophoresis with Laser-Induced Fluorescence Detection Reviewed

    Bi-Feng Liu, Hideaki Hisamoto and Shigeru Terabe

    J. Chromatogr. A 雑誌   1021 ( 1-2 )   201 - 207   2003.12

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  • Micro integrated chemical systems for general use

    Yoshikuni Kikutani, Akihide Hibara, Hideaki Hisamoto, Manabu Tokeshi, Takehiko Kitamori

    Lab-on-a-Chip: Miniaturized Systems for (Bio) Chemical Analysis and Synthesis   285 - 307   2003.10

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    This chapter provides information on micro and nano-fabrication technologies that have been introduced into chemical and biotechnologies. It also explains molecular and energy transport between multiphase laminar flow in a micro channel. As for chemical synthesis, micro reactors with sub-millimeter-sized micro channels are now eagerly investigated. Though there are some examples of electrokinetically-driven flow control in micro reactors, pressure-driven flow control is more commonly used for micro reactors. The aim is to develop methodology to integrate as many chemical processes as possible on microchips and to make full use of characteristics of micro space. Therefore, pressure-driven flow and developed techniques to operate chemical processing in pressure-driven laminar flow in micro channels are selected and for this microchips made of glass are used. Glass vessels are commonly used in many chemical experiments because of their chemical inertness and optical transparency. In order to realize these micro chemical systems based on multiphase laminar flow, some stabilization techniques for the multiphase flow has been inevitable. A micro channel with guide structures and surface modification to stabilize multiphase flows has been used. The chapter also presents a micro integration methodology, continuous flow chemical processing (CFCP), based on spontaneous motion of molecules in multi-phase laminar flow network in micro channel circuits. © 2003 Elsevier B.V. All rights reserved.

    DOI: 10.1016/B978-044451100-3/50015-2

  • Molecular Design, Characterization, and Application of Multiinformation Dyes for Optical Chemical Sensing IV. Multiinformation Dyes with Extended Spectral Sensitivity in the Near-Infrared Spectral Range Reviewed

    Daniel Citterio, Takashi Kawada, Jun Yagi, Tomonori Ishigaki, Hideaki Hisamoto, Shin-ichi Sasaki, Koji Suzuki

    Anal. Chim. Acta 雑誌   482 ( 1 )   19 - 28   2003.04

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  • Phase-transfer Alkylation Reactions Using Microreactors Reviewed

    Masaharu Ueno, Hideaki Hisamoto, Takehiko Kitamori and Shu_ Kobayashi

    Chem. Commun. 雑誌   ( 8 )   936 - 937   2003.04

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  • Molecular design, characterization, and application of multiinformation dyes for optical chemical sensing: IV. Multiinformation dyes with extended spectral sensitivity in the near-infrared spectral range

    Daniel Citterio, Takashi Kawada, Jun Yagi, Tomonori Ishigaki, Hideaki Hisamoto, Shin Ichi Sasaki, Koji Suzuki

    Analytica Chimica Acta   482 ( 1 )   19 - 28   2003.04( ISSN:0003-2670

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    In this paper, new "multiinformation dyes" (MIDs) with extended spectral sensitivity reporting on both changes in solvent polarity (solvatochromism) and in pH (halochromism) by changes in their visible absorption spectra, are presented. The merocyanine dyes 2,6-dibromo-4-[4-(1-dodecyl-4(1H)-pyridinylidene)-2-butenylidene]-2,5- cyclohexadien-1-one (KD-M301) and 2,6-dibromo-4-[4-(1-dodecyl-4(1H)-quinolinylidene)-2-butenylidene]-2,5- cyclohexadien-1-one (KD-M402) were designed, synthesized, and characterized regarding analytical applications. The spectral sensitivity for the MIDs was extended into the near-infrared (NIR) wavelength range (λmax = 800 nm in CHCl3 for KD-M402) and at the same time, the degree of solvent polarity-induced spectral shifts was strongly enhanced (Δλmax = 202 nm for KD-M402 between tetrahydrofuran (THF) and methanol), reaching similar values to the standard solvatochromic dye Dimroth-Reichardt's betaine on which the empirical ET(30) classification of solvent polarity is based. Compared to Dimroth-Reichardt's betaine dye, the molar absorption coefficient ε, is increased more than 10-fold (ε of KD-M402 in THF: 7.7 × 104 M-1 cm-1), allowing sensitive measurements at low concentrations of the dye. The dodecyl-substituted KD-M402 is suitable as a lipophilic pH indicator in ion-exchange type optical sensors (optodes) with optical detection in the near-infrared wavelength range. © 2003 Elsevier Science B.V. All rights reserved.

    DOI: 10.1016/S0003-2670(03)00200-9

  • Chemicofunctional membrane for integrated chemical processes on a microchip

    Hideaki Hisamoto, Yuki Shimizu, Kenji Uchiyama, Manabu Tokeshi, Yoshikuni Kikutani, Akihide Hibara, Takehiko Kitamori

    Analytical Chemistry   75 ( 2 )   350 - 354   2003.01( ISSN:0003-2700

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    Here we report a design and synthesis of a chemically functional polymer membrane by an interfacial polycondensation reaction and multilayer flow inside a microchannel. Single and parallel dual-membrane structures are successfully prepared by using organic/aqueous two-layer flow and organic/aqueous/organic three-layer flow inside the microchannel followed by an interfacial polycondensation reaction. By using the inner-channel membrane, permeation of ammonia species through the inner-channel membrane is successfully achieved. Furthermore, horseradish peroxidase is immobilized on one side of the membrane surface to integrate the chemical transform function onto the inner-channel membrane. Here substrate permeation through the membrane and subsequent chemical transformation at the membrane surface are realized. The polymer membrane prepared inside the microchannel has an important role in ensuring stable contact of different phases such as gas/liquid or liquid/ liquid and the permeation of chemical species through the membrane. Furthermore, membrane surface modification chemistry allows chemical transformation of permeated chemical species. These methods are expected to lead to development of complicated and sophisticated chemical systems involving membrane permeation and chemical reactions.

    DOI: 10.1021/ac025794+

    PubMed

  • Chemico-Functional Membrane for Integrated Chemical Processes on a Microchi Reviewed

    Hideaki Hisamoto, Yuki Shimizu, Kenji Uchiyama, Manabu Tokeshi, Yoshikuni Kikutani, Akihide Hibara, and Takehiko Kitamor

    Anal. Chem. 雑誌   75 ( 2 )   350 - 354   2003.01

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  • An Interface Chip Connection between Capillary Electrophoresis and Thermal Lens Microscope Reviewed

    Kenji Uchiyama, Akihide Hibara, Kiichi Sato, Hideaki Hisamoto, Manabu Tokeshi, Takehiko Kitamori

    Electrophoresis 雑誌   24 ( 1-2 )   179 - 184   2003.01

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  • Fundamental Study on the Segmented Flow Injection - Multiphase Flow Formation Towards Microchip-Based Multi-Ion Sensing

    Hideaki Hisamoto, Takayuki Horiuchi, Akihide Hibara, Manabu Tokeshi, Takehiko Kitamori

    IEEJ Transactions on Sensors and Micromachines   123 ( 4 )   124 - 127   2003( ISSN:1341-8939

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    A new fluid flow inside the microchannel was successfully developed. The flow created here involves segmented flow injection of plural organic phases into a microchannel followed by contact with a single aqueous phase to form stable organic-aqueous two-layer flow inside the microchannel. Fundamental study on the developed flow inside the microchannel was performed by monitoring the dye-doped segmented organic phases by thermal lens microscopy (TLM). Excellent repeatability and very small injection volume in developing segmented flow were realized. The new fluid flow created here is expected to allow us multi-ion sensing, which is not easily demonstrated by conventional ion sensor technology using a solvent polymeric membrane, by combining with neutral ionophore-based ion pair extraction using plural numbers of organic phases containing different ionophore molecules. Keywords : Segmented flow injection, Microchip, μ-TAS, Multi-ion, Multiphase flow. © 2003, The Institute of Electrical Engineers of Japan. All rights reserved.

    DOI: 10.1541/ieejsmas.123.124

  • Glass microchip with three-dimensional microchannel network for 2 × 2 parallel synthesis

    Yoshikuni Kikutani, Takayuki Horiuchi, Kenji Uchiyama, Hideaki Hisamoto, Manabu Tokeshi, Takehiko Kitamori

    Lab on a Chip   2 ( 4 )   188 - 192   2002.11( ISSN:1473-0197

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    An integrated multireactor system for 2 × 2 parallel organic synthesis has been developed on a single glass microchip. Three-dimensional channel circuits in the chip were fabricated by laminating three glass plate layers. The fabrication method is a straightforward extension of the conventional one, and topological equivalence for any three-dimensional circuits can be constructed easily with it. 2 × 2 phase-transfer amide formation reactions, which constitute a simple model for combinatorial synthesis, were successfully carried out on the microchip, and the integrity of the three-dimensional circuits was confirmed. Combinatorial chemistry with multi-microreactors, in conjunction with a high-throughput screening method based on μ-TAS technologies, is expected to provide an efficient tool for drug discovery.

    DOI: 10.1039/b208382p

    PubMed

  • Glass Microchip with Three-Dimensional Microchannel Network for 2x2 Parallel Synthesis Reviewed

    Yoshikuni Kikutani, Takayuki Horiuchi, Kenji Uchiyama, Hideaki Hisamoto, Manabu Tokeshi and Takehiko Kitamori

    Lab on a Chip, 雑誌   ( 4 )   188 - 192   2002.11

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  • Pile-up Glass Microreactor Reviewed

    oshikuni Kikutani, Akihide Hibara, Kenji Uchiyama, Hideaki Hisamoto, Manabu Tokeshi and Takehiko Kitamori

    Lab on a Chip, 雑誌   ( 4 )   193 - 196   2002.11

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  • Comparison of two molecular design strategies for the development of an ammonium ionophore more highly selective than nonactin

    Shin ichi Sasaki, Tsuyoshi Amano, Gou Monma, Takeshi Otsuka, Naoko Iwasawa, Daniel Citterio, Hideaki Hisamoto, Koji Suzuki

    Analytical Chemistry   74 ( 18 )   4845 - 4848   2002.09( ISSN:0003-2700

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    A series of ionophores for ammonium ion-selective electrodes was designed and synthesized, and their characteristics were examined. The design of the ionophores is based on two different strategies: (1) introduction of bulky blocking subunits (decalino groups) in 20- or 21-membered crown ethers (TD20C6 and TD21C6), the ring size of which is expected to be suitable for selective NH4+ recognition, as compared to the slightly smaller K+; and (2) preorganized tripodal ionophores based on a 6-fold substituted benzene ring in order to complementarily recognize the tetrahedral NH4+, in contrast to the spherical K+. Compared to nonactin, a natural product that is used as a representative NH4+ ionophore, the newly developed TD20C6 showed higher NH4+ selectivity over K+ while retaining the selectivity over Na+(log KNH4+,K+pot≡ -1.5 and log KNH4+,Na+pot = -2.5). On the other hand, a tripodal ionophore with pyrazole nitrogen atoms as NH4+ binding sites showed high NH4+/K+. selectivity but suffered from increased Ca2+ interference (log KNH4+,K+pot = -2.1 and log KNH4+,Ca2+pot = -1.6). As an overall conclusion, the cyclic ionophores TD19C6 and TD20C6 are the best ammonium-selective ionophores developed to date.

    DOI: 10.1021/ac025713+

  • Comparison of Two Molecular Design Strategies for the Development of a More Highly Selective Ammonium Ionophore Than Nonactin Reviewed

    Shin-ichi Sasaki, Tsuyoshi Amano, Gou Monma, Takeshi Otsuka, Naoko Iwasawa, Daniel Citterio, Hideaki Hisamoto, and Koji Suzuki

    Anal. Chem. 雑誌   74 ( 18 )   4845 - 4848   2002.09

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  • Three-Layer Flow Membrane System on a Microchip for Investigation of Molecular Transport Reviewed

    Mariana Surmeian, Maxim N. Slyadnev, Hideaki Hisamoto, Akihide Hibara, Kenji Uchiyama, Takehiko Kitamor

    Anal. Chem. 雑誌   74 ( 9 )   2014 - 2020   2002.05

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  • Continuous Flow Chemical Processing on a Microchip by Combining Micro Unit Operations and a Multiphase Flow Network Reviewed

    Manabu Tokeshi, Tomoko Minagawa, Kenji Uchiyama, Akihide Hibara, Kiichi Sato, Hideaki Hisamoto, and Takehiko Kitamori

    Anal. Chem. 雑誌   74 ( 7 )   1565 - 1571   2002.04

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  • Design and synthesis of Mg<sup>2+</sup>-selective fluoroionophores based on a coumarin derivative and application for Mg<sup>2+</sup> measurement in a living cell

    Yoshio Suzuki, Hirokazu Komatsu, Takafumi Ikeda, Naohiko Saito, Sawa Araki, Daniel Citterio, Hideaki Hisamoto, Yoshiichiro Kitamura, Takeshi Kubota, Jun Nakagawa, Kotaro Oka, Koji Suzuki

    Analytical Chemistry   74 ( 6 )   1423 - 1428   2002.03( ISSN:0003-2700

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    Novel Mg2+ fluorescent molecular probes (KMG-20-AM and KMG-27-AM; where AM is an acetoxymethyl group) based on a coumarin possessing a charged β-diketone structure were designed and synthesized. These fluorescent probes produced a red shift from 425 to 445 nm in the absorption spectra after formation of a complex with Mg2+. The fluorescence spectra of these probes also showed a red shift from 485 to 495 nm and an increasing fluorescence intensity after formation of a complex with Mg2+. The optimum experimental conditions were excitation wavelength of 445 nm and a monitored wavelength of 500 nm, where these probes functioned as an indicator showing an image of increasing fluorescence in the presence of Mg2+. These probes showed a "seesaw-type" fluorescent spectral change with the isosbestic point at 480 nm due to the light excitation at 445 nm, which indicates that ratiometry can be used for the measurement. The molecular probes formed a 1:1 complex with Mg2+ and the dissociation constant (Kd) was 10.0 mM for KMG-20. The association constants of the probes with Mg2+ were ∼3 times higher than that with Ca2+, which showed that the selectivity of Mg2+ versus Ca2+ for these probes was over 200 times higher than that for commercially available Mg2+ fluorescent molecular probes such as mag-fura-2, Magnesium Green. As an application of these probes, intracellular fluorescent imaging of Mg2+ was demonstrated using a fluorescent microscope. After the addition of KMG-20-AM and KMG-27-AM into PC12 cells, a strong fluorescence was observed in the cytoplasm and a weak fluorescence in the nuclei region. After treatment with a high-K+ medium, the fluorescence intensity increased due to increasing intracellular Mg2+. The real image of Mg2+ release from the magnesium store was successfully observed with these Mg2+ fluorescent probes.

    DOI: 10.1021/ac010914j

    PubMed

  • Design and Synthesis of Mg2+ Selective Fluoroionophores Based on Coumarin Derivative and Application for the Mg2+ Measurement in a Living Cell Reviewed

    Yoshio Suzuki, Hirokazu Komatsu, Takafumi Ikeda, Naohiko Saito, Sawa Araki, Daniel Citterio, Hideaki Hisamoto, Yoshiichiro Kitamura, Takeshi Kubota, Jun Nakagawa, Kotaro Oka, and Koji Suzuki

    Anal. Chem. 雑誌   74 ( 6 )   1423 - 1428   2002.03

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  • Fast and High Conversion Phase-Transfer Synthesis Exploiting Liquid/Liquid Interface Formed in Microchannel Chip Reviewed

    Hideaki Hisamoto, Takumi Saito, Manabu Tokeshi, Akihide Hibara, and Takehiko Kitamori

    Chem. Commun. 雑誌   ( 24 )   2662 - 2663   2001.12

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  • Optical Determination of Low-Level Water Concentrations in Organic Solvents Using Fluorescent Acridinyl Dyes and Dye-Immobilized Polymer Membranes Reviewed

    Daniel Citterio, Katsuya Minamihashi, Yuka Kuniyoshi, Hideaki Hisamoto, Shin-ichi Sasaki, and Koji Suzuki

    Anal.Chem. 雑誌   73 ( 21 )   5339 - 5345   2001.11

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  • On-Chip Integration of Sequential Ion Sensing System Based on Intermittent Reagent Pumping and Formation of Two-Layer Flow Reviewed

    Hideaki Hisamoto, Takayuki Horiuchi, Kenji Uchiyama, Manabu Tokeshi, Akihide Hibara, and Takehiko Kitamori

    Anal.Chem. 雑誌   73 ( 22 )   5551 - 5556   2001.11

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  • Synthesis of Novel Hg2+ Receptors Based on N-Benzyloxyamide Derivatives and Their Application to Anion-Selective Electrodes Reviewed

    Shin-ichi Sasaki, Tsuyoshi Amano, Satoru Ozawa, Tomomi Masuyama, Daniel Citterio, Hideaki Hisamoto, Hisao Hori, and Koji Suzuki

    J. Chem.Soc., Perkin Trans 1 雑誌   ( 12 )   1366 - 1371   2001.05

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  • Thermal lens microscope for integrated chemistry laboratory on glass microchips Reviewed

    A Hibara, K Sato, H Hisamoto, K Uchiyama, MN Slyadnev, M Tokeshi, T Kitamori

    PROGRESS IN NATURAL SCIENCE   11   S237 - S241   2001.05( ISSN:1002-0071

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    Thermal lens microscope (TLM) can be a powerful tool for a detection method for integrated chemistry laboratory (ICL). In spite of great potential of ICL, its application has been restricted due to the lack of highly applicable and sensitive detection methods. TLM developed by our group meets requirements of detection method for ICL, such as high sensitivity, high spatial resolution, and wide applicability. In this review, the principle and characteristics of TLM are introduced and usefulness of TLM in ICL is demonstrated in some analytical applications.

  • Stabilization of Liquid Interface and Control of Two-Phase Confluence and Separation in Glass Microchips by Utilizing Octadecylsilane Modification of Microchannels Reviewed

    Akihide HibaraD, Masaki Nonaka, Hideaki Hisamoto, Kenji Uchiyama, Yoshikuni Kikutani,Manabu Tokeshi, and Takehiko Kitamori

    Anal. Chem. 雑誌   74 ( 7 )   1724 - 1728   2001.04

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  • On-Chip Integration of Neutral Ionophore-Based Ion Pair Extraction Reaction Reviewed

    Hideaki Hisamoto, Takayuki Horiuchi, Akihide Hibara, Manabu Tokeshi, and Takehiko Kitamori

    Anal. Chem. 雑誌   73 ( 6 )   1382 - 1386   2001.03

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  • Micrometer-sized lithium ion-selective microoptodes based on a 'tailed' neutral ionophore and a fluorescent anionic dye

    Kazuyoshi Kurihara, Motoichi Ohtsu, Takeo Yoshida, Toshihito Abe, Hideaki Hisamoto, Koji Suzuki

    Analytica Chimica Acta   426 ( 1 )   11 - 18   2001.01( ISSN:0003-2670

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    The preparation and response features of a micrometer-sized lithium ion-selective optode based on a liquid membrane were examined. The optode membrane was a plasticized poly(vinyl chloride) (PVC)-based copolymer incorporating a lipophilic 14-crown-4 derivative as the neutral lithium ionophore and a dibromofluorescein derivative as the fluorescent anionic dye. The detection mode was a fluorescence change based on the ion-pair extraction/ion exchange principle caused by the fluorescent anionic dye and the lithium ionophore. The 5-μm-sized microoptode was prepared by the micropipette fabrication method and characterized by measuring the optical responses to Li+ concentrations with the time-resolved photon counting method. The microoptode responded to lithium ion concentrations of ~0.5 to ~500mM. The micrometer-sized lithium ion-selective microoptode was successfully obtained when a tailed ionophore was used. The anchor effect of the tailed ionophore was useful for the lithium ion-selective microoptode to resolve leaching of the ionophore, which is a significant problem in a microoptode based on a liquid membrane. This demonstration indicates that other ion-selective microoptodes can be obtained simply by replacing the tailed ionophore. (C) 2001 Elsevier Science B.V.

    DOI: 10.1016/S0003-2670(00)01148-X

  • Micrometer-Sized Lithium Ion-Selective Microoptodes Based on a "Tailed" Neutral Ionophore and a Fluorescent Anionic Dye Reviewed

    Kazuyoshi Kurihara, Motoichi Ohtsu, Takeo Yoshida, Toshihito Abe, Hideaki Hisamoto, and Koji Suzuki

    Anal. Chim. Acta 雑誌   426 ( 1 )   11 - 18   2001.01

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  • Integrated Multilayer Flow System on a Microchip Reviewed

    Akihide HibaraD, Manabu Tokeshi, Kenji Uchiyama, Hideaki Hisamoto, and Takehiko Kitamori

    Anal. Sci. 雑誌   17   89 - 93   2001.01

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  • Distribution of Methyl Red on Water-Organic Liquid Interface in Microchannel Reviewed

    Mariana Surmeian, Akihide Hibara, Maxim N. Slyadnev, Kenji Uchiyama, Hideaki Hisamoto, Takehiko Kitamori

    Anal. Lett. 雑誌   34 ( 9 )   1421 - 1429   2001

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  • Distribution of methyl red on the water-organic liquid interface in a microchannel

    Mariana Surmeian, A. Hibara, M. Slyadnev, K. Uchiyama, H. Hisamoto, T. Kitamori

    Analytical Letters   34 ( 9 )   1421 - 1429   2001( ISSN:0003-2719

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    Molecular transport across a water-organic liquid interface formed parallel to the side wall of a microchannel on microchips has been studied using methyl red as model compound and cyclohexane and n-octanol as organic solvents. The molecular transport was monitored by thermal lens microscopy and the kinetics of the process was studied for different concentrations and flow velocities. The influence of physico-chemical interactions between the model compound and organic solvents on liquid-liquid interface was demonstrated in the microspace and corresponding partition coefficients were calculated which confirmed the experimental results.

    DOI: 10.1081/AL-100104916

  • Synthesis of novel Hg<sup>2+</sup> receptors based on N-benzyloxyamide derivatives and their application to anion-selective electrodes

    S. I. Sasaki, T. Amano, S. Ozawa, T. Masuyama, D. Citterio, H. Hisamoto, H. Hori, K. Suzuki

    Journal of the Chemical Society. Perkin Transactions 1   ( 12 )   1366 - 1371   2001( ISSN:1472-7781

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    Novel Hg2+ receptors having two N-benzyloxyamide groups are synthesized, and their complexation ability toward metal cations is examined. The structure of the formed complexes was elucidated by ESI-MS and 1H NMR spectroscopy. Deprotonation of the amide groups allows the receptors to form stable complexes with Hg2+. As an application of this neutral Hg2+ complex, an anion-selective electrode combined with a cationic additive is prepared, and exhibits an anti-Hofmeister selectivity pattern with enhanced selectivity for the iodide anion. The overall selectivity pattern of the electrode is I- > ClO4- > SCN- ≈ Br- > salicylate- > NO2- > Cl- > NO3- ≫ HCO3- > SO42- > F- > H2PO4-.

    DOI: 10.1039/b008978h

  • Fast and high conversion phase-transfer synthesis exploiting the liquid–liquid interface formed in a microchannel chip

    Hideaki Hisamoto, Takumi Saito, Manabu Tokeshi, Akihide Hibara, Takehiko Kitamori

    Chemical Communications   1 ( 24 )   2662 - 2663   2001( ISSN:1359-7345

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    The large specific interfacial areas and short molecular diffusion distances provided by glass microchips play important roles not only for effective phase-transfer synthetic reaction, but also for avoiding an undesirable side reaction. © 2001 The Royal Society of Chemistry.

    DOI: 10.1039/b106494k

  • Design and Synthesis of a More Highly Selective Ammonium Ionophore Than Nonactin and Its Application as an Ion-Sensing Component for an Ion-Selective Electrode Reviewed

    Koji Suzuki, Dwi Siswanta, Takeshi Otsuka, Tsuyoshi Amano, Takafumi Ikeda, Hideaki Hisamoto, Ryoko Yoshihara, and Shigeru Ohba

    Anal. Chem. 雑誌   72 ( 10 )   2200 - 2205   2000.05

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    Kind of work:Joint Work  

  • Visual and Colorimetric Lithium Ion Sensing Based on Digital Color Analysis Reviewed

    Etsuko Hirayama, Tsunemi Sugiyama, Hideaki Hisamoto, and Koji Suzuki

    Anal. Chem. 雑誌   72 ( 3 )   465 - 474   2000.02

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  • Ultrasmall ion-selective fiber optodes

    Kazuyoshi Kurihara, Motoichi Ohtsu, Hideaki Hisamoto, Koji Suzuki

    Bunseki Kagaku   49 ( 12 )   961 - 967   2000( ISSN:0525-1931

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    The preparation and response features of a micrometer-sized sodium ion-selective fiber optode based on a liquid membrane were described. The sensing membrane is a plasticized poly (vinyl chloride)-based copolymer with a neutral ionophore and an anionic dye. In order to fabricate a micrometer-sized fiber optode, a "micropipette fabrication method" was newly proposed to fix a liquid membrane-based optode on the small tip of an optical fiber probe. At the first stage of the investigation, it was found that the ionophore including a sodium ion in its cavity leached from the membrane phase. However, we have discovered that the problem can be resolved by using a "tailed" ionophore, which is an ionophore possessing a lipophilic long alkyl chain. The "'tail" of the ionophore functions as an "anchor", which prevents leaching of die ionophore from the membrane phase into the water phase. The anchor effect of the tailed ionophore was clearly demonstrated with 6 μm-sized sodium ion-selective optodes. In addition, the problem of fluorescence distortion due to photobleaching and solvent effect was resolved by a ratiometric calibration in which the sensor response is monitored by the spectral shift of the dual-emission fluorescence. The sensor response of an 8 μm-sized fiber optode having ratiometric calibration was examined and successfully explained by response theory. Our method gives a general preparation for ultrasmall ion-selective fiber optodes because other ion-selective optodes can be obtained simply by replacing the tailed ionophore.

    DOI: 10.2116/bunsekikagaku.49.961

  • Theory and practice of rapid flow-through analysis based on ion-selective optode detection and its application to sodium ion determination

    Hideaki Hisamoto, Sayaka Satoh, Kazunari Satoh, Michiko Tsubuku, Dwi Siswanta, Yushi Shichi, Yasuhiro Koike, Koji Suzuki

    Analytica Chimica Acta   396 ( 2-3 )   131 - 141   1999.09( ISSN:0003-2670

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    One simple example of a rapid flow-through analysis (RFA) system based on ion-selective optode detection was demonstrated, and its performance was discussed. The RFA is a flow analytical method based on a reagentless analyte flow injection system combined with an analyte selective monitor/sensor such as an optical chemical sensor (optode) involving mathematical response simulation using a semi-empirical theoretical response equation. The response of RFA with optode detection can basically be expressed by the equation derived from Fick's law of diffusion and Kubelka-Munk's law, and two other variable parameters in relation to the experimental response. Mathematically simulated results using the proposed equation indicated that the equation can be useful for the prediction of the flow analytical response. In the present demonstration of RFA, highly selective ionophores based on 16-crown-5 derivatives were used for the ion-sensing component of the optode for sodium ion determination. A sodium ion-selective optode based on C14-DD16C5 and KD-A3 exhibited high selectivity for sodium ion over potassium ion, with a selectivity coefficient (logK(i,j)(opt)) of -2.6. This optode responded to sodium ion in the concentration range from 10-5 to 10-1M at pH 8.0 by measuring the absorbance change at 510nm in the sensing phase of the optode. The RFA system equipped with a sodium ion-selective optode offers a reagent-less advanced ion-determination methodology for conventional flow injection analysis. Copyright (C) 1999 Elsevier Science B.V.

    DOI: 10.1016/S0003-2670(99)00482-1

  • Theory and Practice of Rapid Flow-Through Analysis (RFA) 2; RFA Based on Ion-Selective Optode Detection and its Application to Sodium Ion Determination Reviewed

    Hideaki Hisamoto, Sayaka Satoh, Kazunari Satoh, Michiko Tsubuku, Dwi Siswanta, Yushi Shichi, Yasuhiro Koike, and Koji Suzuki

    Anal. Chim. Acta 雑誌   396 ( 2-3 )   131 - 141   1999.09

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  • Micrometer-sized sodium ion-selective optodes based on a 'tailed' neutral ionophore

    Kazuyoshi Kurihara, Motoichi Ohtsu, Takeo Yoshida, Toshihito Abe, Hideaki Hisamoto, Koji Suzuki

    Analytical Chemistry   71 ( 16 )   3558 - 3566   1999.08( ISSN:0003-2700

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    The preparation and response features of a micrometer-sized sodium ion- selective fiber optode based on a liquid membrane were described. The sensing membrane is a plasticized poly(vinyl chloride)-based copolymer with a neutral sodium ionophore and an anionic dye. To fabricate a micrometer-sized fiber optode, a 'micropipet fabrication method' was proposed to fix a liquid membrane-based optode on the small tip of an optical fiber probe, in which the optode size can be varied from ~1 μm to more than 10 μm. Our first optode incorporated a 16-crown-5 derivative as the neutral sodium ionophore and a dibromofluorescein derivative as the single-emission anionic dye. The sensor response was monitored by measuring the fluorescence intensity under a time-resolved photon-counting method. At the first stage of the investigation, it was found that the ionophore including a sodium ion in its cavity leached from the membrane phase. However, we have discovered that the problem can be resolved by using a 'tailed' ionophore, which is an ionophore possessing a lipophilic long alkyl chain. The 'tail' of the ionophore functions as an 'anchor' that prevents leaching of the ionophore from the membrane phase into the water phase. The anchor effect of the tailed ionophore was clearly demonstrated with 6-μm-sized sodium ion-selective optodes. Using a 3-μm-sized optode, the sensor response was examined and successfully explained by the response theory. The size limitation of the optode was also examined using the response features of a 1.5-μm-sized fiber optode. The second optode incorporated a tailed 16-crown-5 derivative as the neutral sodium ionophore and a coumarin derivative as the dual-emission anionic dye. The second optode is more practical than the first optode because the problem of fluorescence distortion due to photobleaching and solvent effect is resolved by ratiometric calibration where the sensor response is monitored by the spectral shift of the dual-emission fluorescence. The sensor response of an 8-μm-sized fiber optode having ratiometric calibration was examined and successfully explained by the response theory.

    DOI: 10.1021/ac981206+

  • Micrometer-Sized Sodium Ion-Selective Optodes Based on a "Tailed" Neutral Ionophore Reviewed

    Kazuyoshi Kurihara, Motoichi Ohtsu, Takeo Yoshida, Toshihito Abe, Hideaki Hisamoto, and Koji Suzuki

    Anal. Chem. 雑誌   71 ( 16 )   3558 - 3566   1999.08

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  • Ion-selective optodes: Current developments and future prospects

    Hideaki Hisamoto, Koji Suzuki

    TrAC - Trends in Analytical Chemistry   18 ( 8 )   513 - 524   1999.08( ISSN:0165-9936

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    The chemistry of the ion-selective optode has recently been recognized as a highly selective ion-sensing methodology which involves a highly selective complexation between an ionophore and a primary ion. This simple methodology allowed the construction of several kinds of ion-sensing devices which enabled highly selective ion determination for analytical purposes. In this review, sensing devices are divided into the following four types with different analytical uses: (1) flow analytical devices (flow-through optode); (2) waveguide devices (waveguide optode); (3) sensing plate devices (sensing plate optode, film optode); (4) fiber-optic devices (fiber optode). The characteristics of each device are explained and discussed with experimental data, and the future prospects for ion-selective optode methodology are described. Copyright (C) 1999 Elsevier Science B.V.

    DOI: 10.1016/S0165-9936(99)00137-5

  • Molecular Design, Characterization, and Application of Multiinformation Dyes for Multi-Dimensional Optical Chemical Sensings.3. Application of MIDs for ?max-Tunable Ion-Selective Optodes Reviewed

    Hideaki Hisamoto, Mihoko Tani, Sachiko Mori, Toshiki Yamada, Tomonori Ishigaki, Hajime Tohma, and Koji Suzuki

    Anal. Chem. 雑誌   71 ( 1 )   259 - 264   1999.01

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  • Molecular design, characterization, and application of multiinformation dyes (MIDs) for optical chemical sensings. 3. Application of MIDs for λ<inf>max</inf>-tunable ion-selective optodes

    Hideaki Hisamoto, Mihoko Tani, Sachiko Mori, Toshiki Yamada, Tomonori Ishigaki, Hajime Tohma, Koji Suzuki

    Analytical Chemistry   71 ( 1 )   259 - 264   1999( ISSN:0003-2700

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    By utilizing "multiinformation dyes (MIDs)", which have plural spectral change characteristics such as an absorption maximum wavelength (λmax) shift based on a polarity change and an absorbance change due to protonation, novel λmax-tunable ion-selective optodes were proposed and prepared by employing MIDs with membrane solvents having different polarities. For controlling the detecting λmax of the optode, the novel polar membrane solvent [2-[[6-(2-nita)phenoxy)hexyl]oxy]methyl]isobutane-1,3-diol was designed and synthesized, which was used together with a typical membrane solvent nitrophenyl octyl ether. By mixing these two membrane solvents, the λmax position of the optode detection wavelength can be shifted and controlled and was successfully applied to a λmax-tunable Li+-selective optode based on a highly Li+-selective ionophore TTD14C4. The λmax tuning technique is useful for preparing an optode system using a low-cost light source such as a light-emitting diode or a popular laser. © 1998 American Chemical Society.

    DOI: 10.1021/ac980757x

  • Molecular design, characterization, and application of multi-information dyes for multi-dimensional optical chemical sensing. Molecular design concepts of the dyes and their fundamental spectral characteristics

    Hideaki Hisamoto, Hajime Tohma, Toshiki Yamada, Ki Ichiro Yamauchi, Dwi Siswanta, Naoki Yoshioka, Koji Suzuki

    Analytica Chimica Acta   373 ( 2-3 )   271 - 289   1998.11( ISSN:0003-2670

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    The molecular design of multi-functional dyes, called 'multi-information dyes' (MIDs), and their application to several methods of optical chemical sensing were proposed. The concepts for the molecular design of the dyes were as follows: (1) the introduction of multiple (both positive and negative) charges on both ends of the large conjugated π-electron system of the dye molecule, so that the dye interacts with many chemical species or environments; (2) the introduction of different substitution (electron-donating or electron-accepting) groups in the conjugated π-electron system of the dye molecule, so that the dye has a different pK(a) and solvatochromic property; and (3) the introduction of an immobilization site in the dye molecule, so that the dye can be easily prepared as a sensing probe.Based on these concepts, sixteen kinds of dyes were synthesized, all being of the merocyanine type. The basic color-change characteristics of the MIDs were evaluated with a homogeneous system and a solvent extraction two-phase system. Some of these dyes (e.g., KD-M5) offered two-dimensional sensing information for sensing the water content in organic solvents, in which the absorbance maximum wavelength (λ(max)) shift and absorbance change were both utilized as the detecting signals.From these results, the MIDs developed in this study have several possibilities for analytical applications in optical chemical sensing such as pH sensing, ion sensing, neutral molecule sensing, and water content-sensing in organic solvents. Copyright (C) 1998 Elsevier Science B.V.

    DOI: 10.1016/S0003-2670(98)00421-8

  • Molecular Design, Characterization, and Application of Multiinformation Dyes for Multi-Dimensional Optical Chemical Sensings.1. Molecular Design Concepts of the Dyes and Their Fundamental Spectral Characteristics Reviewed

    Hideaki Hisamoto, Hajime Tohma, Toshiki Yamada, Ki-ichiro Yamauchi, Dwi Siswanta and Koji Suzuki

    Anal. Chim. Acta 雑誌   373 ( 2-3 )   271 - 289   1998.11

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  • Molecular Design, Characterization, and Application of Multiinformation Dyes for Multi-Dimensional Optical Chemical Sensings.2. Preparation of the Optical Sensing Membranes for the Simultaneous Measurements of pH and Water Content in Organic Media Reviewed

    Hideaki Hisamoto, Yukiko Manabe, Hiroko Yanai, Hajime Tohma, Toshiki Yamada, and Koji Suzuki

    Anal. Chem. 雑誌   70 ( 7 )   1255 - 1261   1998.04

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  • Preparation of Sodium Ion-Sensing Plates Based on a Thin Layer Liquid Membrane Containing a Neutral Ionophore and a Lipophilic Anionic Dye Reviewed

    Hideaki Hisamoto, Sayaka Sato, Kazunari Sato, Dwi Siswanta and Koji Suzuki

    Anal.Sci. 雑誌   14 ( 1 )   127 - 131   1998.02

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  • Magnesium Ion-Selective Optodes Based on a Neutral Ionophore and a Lipophilic Cationic Dye Reviewed

    Dwi Siswanta, Hideaki Hisamoto, Sayaka Sato, Yukihiro Matsumoto, Yasuhiro Koike, Shinji Yamamori, and Koji Suzuki

    Anal.Sci. 雑誌   13 ( 3 )   429 - 435   1997.06

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  • Active optical thin-film waveguide sensor for ion sensing

    Kyungho Kim, Haruyuki Minamitani, Hideaki Hisamoto, Koji Suzuki, Shinwon Kang

    Analytica Chimica Acta   343 ( 3 )   199 - 208   1997.05( ISSN:0003-2670

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    In this paper, an active PVC optical thin-film waveguide sensor is proposed which can select a specific ion and measure its concentration. The waveguide was fabricated using the plasticized poly(vinyl chloride) (PVC), bis ethyl hexyl phthalate (BEHP), lipophilic anionic dye (LAD-3), and ionophores (K23E1 for calcium, or C14DD16C5 for sodium) in which the PVC membrane plays important roles for both incident light propagation and selective ion sensing. In order to investigate the applicability of this sensor to Ca2+ and Na+ measurement, the sensor was applied to sample solutions whose CaCl2 concentration was gradually altered. Also, we applied it to a NaCl solution. In this experiment, we found that this PVC thin-film waveguide possessed good light propagation for argon laser light at 488 nm. Also, the comparison of the experimental responses between the active waveguide sensor and a commonly utilized waveguide sensor (evanescent waveguide spectrometry), indicated that the sensitivity of the active waveguide sensor is obviously higher than that of the conventional waveguide sensor. These findings suggested that the proposed PVC waveguide ion sensor is applicable to the determination of various ions with high sensitivity.

    DOI: 10.1016/S0003-2670(97)00006-8

  • Ion Sensitive and Selective Active Waveguide Optodes Reviewed

    Hideaki Hisamoto, Kyung-Ho Kim, Yukiko Manabe, Keisuke Sasaki, Haruyuki Minamitani and Koji Suzuki

    Anal. Chim. Acta 雑誌   342 ( 1 )   31 - 39   1997.04

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  • Novel Neutral Anion Ionophores Based on Fluorinated (Poly) Ether Compounds as a Sensory Molecule for an Ion-Selective Electrode Reviewed

    Dwi SiswantaD, Jun Takenaka, Takeshi Suzuki, Hideshi Sasakura, Hideaki Hisamoto and Koji Suzuki

    Chem.Lett. 雑誌   26 ( 3 )   195 - 196   1997.03

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  • Structural Ion Selectivity of Thia Crown Ether Compounds with a Bulky Block Subunit and their Application as an Ion Sensing Component for an Ion-Selective Electrode Reviewed

    Dwi SiswantaD, Kazuhisa Nagatsuka, Hiroyuki Yamada, Kazuhiro Kumakura, Hideaki Hisamoto, Yushi Shichi, Kazuaki Toshima, and Koji Suzuki

    Anal.Chem. 雑誌   68 ( 23 )   4166 - 4172   1996.12

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  • Theory and Practise of Rapid Flow-Through Analysis Based on Optode Detection and Its Application to pH Measurement as a Model Case

    Hideaki Hisamoto, Michiko Tsubuku, Takuya Enomoto, Kazuhiko Watanabe, Haruma Kawaguchi, Yasuhiro Koike, Koji Suzuki

    Analytical Chemistry   68 ( 21 )   3871 - 3878   1996.11( ISSN:0003-2700

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    Rapid flow-through analysis (RFA) based on optode (optical chemical sensor) detection is proposed, and its performance is discussed using the RFA system equipped with a pH-sensitive optode as a model case. To demonstrate and understand the usefulness of the RFA system, long-lifetime pH-sensitive optodes were prepared using a hydrophilic poly(hydroxyethyl methacrylate) (poly-HEMA) membrane covalently immobilized with a dye containing an amino group such as Congo Red or Nile Blue. The response equation for the optode with the RFA system was proposed, and satisfactory estimation for rapid pH analysis was obtained. The proposed RFA can stand as an advanced method for conventional flow injection analysis.

    DOI: 10.1021/ac951149+

  • Theory and Practice of Rapid Flow-Through Analysis Based on Optode Detection and its Application to pH measurement as a Model Case Reviewed

    Hideaki Hisamoto, Michiko Tsubuku, Takuya Enomoto, Kazuhiko Watanabe, Haruma Kawaguchi, Yasuhiro Koike, and Koji Suzuki

    Anal.Chem. 雑誌   68 ( 21 )   3871 - 3878   1996.11

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  • Novel Hg2+-Ionophores Based on N-Hydroxylamide Derivatives as a Sensory Molecule for an Ion-Selective Electrode Reviewed

    Dwi SiswantaD, Masanori Kin, Hideaki Hisamoto, and Koji Suzuki

    Chem.Lett. 雑誌   25 ( 11 )   1011 - 1012   1996.11

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  • POFChemicalSensors

    HISAMOTO Hideaki, SUZUKI Koji

    Kobunshi   45 ( 2 )   100 - 100   1996.02( ISSN:0454-1138

  • Design and Synthesis of Sodium Ion-Selective Ionophores Based on 16-Crown-5 Derivatives for an Ion-Selective Electrode Reviewed

    Koji Suzuki, Kazunari Sato, Hideaki Hisamoto, Dwi Siswanta, Kazuo Hayashi, Noriko Kasahara, Kazuhiko Watanabe, Noriko Yamamoto and Hideshi Sasakura

    Anal.Chem. 雑誌   68 ( 1 )   208 - 215   1996.01

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  • Novel Hg<sup>2+</sup>-ionophores based on N-hydroxylamide derivatives as a sensory molecule for an ion-selective electrode

    Dwi Siswanta, Masanori Kin, Hideaki Hisamoto, Koji Suzuki

    Chemistry Letters   ( 11 )   1011 - 1012   1996( ISSN:0366-7022

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    Several N-hydroxylamide derivatives were developed, exhibiting high selectivity for Hg2+, which are useful as sensory molecules for a potentiometric ion sensor.

    DOI: 10.1246/cl.1996.1011

  • Ion Sensing Film Optodes: disposable ion sensing probes for the determination of Na+, K+, Ca2+ and Cl- concentrations in serum Reviewed

    Hideaki Hisamoto, Nami Miyashita, Kazuhiko Watanabe, Eriko Nakagawa and Koji Suzuki

    Sens.Actuators B. 雑誌   29 ( 1-3 )   378 - 385   1995.10

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  • Ion sensing film optodes: disposable ion sensing probes for the determination of Na<sup>+</sup>, K<sup>+</sup>, Ca<sup>2+</sup> and Cl<sup>-</sup> concentrations in serum

    Hideaki Hisamoto, Nami Miyashita, Kazuhiko Watanabe, Eriko Nakagawa, Noriko Yamamoto, Koji Suzuki

    Sensors and Actuators: B. Chemical   29 ( 1-3 )   378 - 385   1995.10( ISSN:0925-4005

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    Highly selective ion sensing film optodes for the determination of four kinds of ions (Na+, K+, Ca2+, Cl-) were prepared with plasticized PVC membranes containing the respective ionophores and a newly prepared lipophilic anionic dye. The principle of the optical response of these sensing films is based on ion-pair extraction of the ion-ionophore complex and a deprotonated or protonated lipophilic anionic dye. These film optodes are for disposable use and can detect ions by simply immersing them into a glass vessel which is filled with the samples solution and determining the ion by measuring the absorbance change at the respective maximum absorption wavelength of the lipophilic anionic dyes in the visible region. The theoretical response of these films was also discussed based on the ion-extraction model. The ion selectivity of these films was sufficient for the determination of Na+, K+, Ca2+ and Cl- in diluted serum samples, and satisfactory results were obtained in serum analysis. © 1995.

    DOI: 10.1016/0925-4005(95)01711-9

  • Silver Ion Selective Optodes Based on Novel Thia Ether Compounds Reviewed

    Hideaki Hisamoto, Eriko Nakagawa, Kazuhisa Nagatsuka, Yoko Abe, Sayaka Sato, Dwi Siswanta and Koji Suzuki

    Anal.Chem. 雑誌   67 ( 8 )   1315 - 1321   1995.04

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  • A Computer Program for Evaluation and Graphical Representation of the Potentiometric Selectivity Coefficients of Ion-Selective Electrodes Reviewed

    Dwi SiswantaD, Hideaki Hisamoto and Koji Suzuki

    Trends Anal.Chem. 雑誌   14 ( 3 )   101 - 104   1995.03

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  • Anion Selective Polymeric Membrane Electrodes Based on Metallocenes Reviewed

    Hideaki Hisamoto, Dwi Siswanta, Hiroshi Nishihara, and Koji Suzuki

    Anal. Chim. Acta 雑誌   304 ( 2 )   171 - 176   1995.03

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  • Design and Synthesis of Calcium and Magnesium Ionophores Based on Double-Armed Diazacrown Ether Compounds and their Application to an Ion Sensing Component for an Ion-Selective Electrode Reviewed

    Koji Suzuki, Kazuhiko Watanabe, Yukihiro Matsumoto, Mitsuru Kobayashi, Sayaka Sato, Dwi Siswanta and Hideaki Hisamoto

    Anal.Chem. 雑誌   67 ( 2 )   324 - 334   1995.01

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  • Flow-through Type Calcium Ion Selective Optodes Based on Novel Neutral Ionophores and a Lipophilic Anionic Dye Reviewed

    Hideaki Hisamoto, Kazuhiko Watanabe, Eriko Nakagawa, Yushi Shichi and Koji Suzuki

    Anal. Chim. Acta 雑誌   299 ( 2 )   179 - 187   1994.12

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  • Flow-through Type Chloride Ion Selective Optodes Based on Lipophilic Organometallic Chloride Adducts and a Lipophilic Anionic Dye Reviewed

    Hideaki Hisamoto, Kazuhiko Watanabe, Hitoshi Oka, Eriko Nakagawa, Ursula E.Spichger, and Koji Suzuki

    Anal.Sci. 雑誌   10 ( 4 )   615 - 622   1994.08

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  • Novel Ammonium Ionophores Based on Glycol Dibenzyl Ethers for an Ion-Selective Electrode Reviewed

    Dwi SiswantaD, Hideaki Hisamoto, Hajime Tohma, Noriko Yamamoto, and Koji Suzuki

    Chem.Lett. 雑誌   23 ( 5 )   945 - 948   1994.05

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  • Design and Synthesis of Highly Selective Ionophores for Lithium Ion Based on 14-Crown-4 Derivatives for an Ion-Selective Electrode Reviewed

    Koji Suzuki, Hiroyuki Yamada, Kazunari Sato, Kazuhiko Watanabe, Hideaki Hisamoto, Yoshito Tobe, and Kazuya Kobiro

    Anal.Chem. 雑誌   65 ( 23 )   3404 - 3410   1993.12

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  • Lithium Ion Selective Optical Sensor Based on a Novel Neutral Ionophore and a Lipophilic Anionic Dye Reviewed

    Kazuhiko Watanabe, Eriko Nakagawa, Hiroyuki Yamada, Hideaki Hisamoto, and Koji Suzuki

    Anal.Chem. 雑誌   65 ( 19 )   2704 - 2710   1993.10

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MISC

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  • 核酸アプタマー高効率選抜のためのミクロスケール電気泳動フィルタリングデバイス

    末吉健志, 末吉健志, 永井伶奈, 遠藤達郎, 久本秀明

    日本分析化学会年会講演要旨集(Web)   69th   2020

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  • 多段階部分的注入キャピラリー電気泳動法による核酸-低分子化合物間相互作用解析

    光野恵理子, 遠藤達郎, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    分析化学討論会講演要旨集(Web)   80th   2020

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  • 多段階部分的注入アフィニティーキャピラリー電気泳動法による疾患関連核酸-低分子化合物間相互作用解析

    光野恵理子, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    キャピラリー電気泳動シンポジウム講演要旨集   40th (CD-ROM)   2020

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  • 多段階部分的注入アフィニティーキャピラリー電気泳動法による物質間相互作用解析

    光野恵理子, 久本秀明, 遠藤達郎, 末吉健志, 末吉健志

    日本分析化学会年会講演要旨集(Web)   69th   2020

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  • Fabrication and characterization of YAG:Ce<sup>3+</sup> phosphor powder contained polymer-based photonic crystal for optical sensor application

    植田渉太郎, 小林奈緒, 川崎大輝, 山田大空, 末吉健志, 末吉健志, 久本秀明, 遠藤達郎, 遠藤達郎

    応用物理学会春季学術講演会講演予稿集(CD-ROM)   67th   2020

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  • ミクロスケール電気泳動フィルタリングに基づく核酸アプタマー高効率選抜デバイスの開発

    飛田安梨沙, 高尾隼空, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    化学とマイクロ・ナノシステム学会研究会講演要旨集   42nd (Web)   2020

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  • ミクロスケール電気泳動フィルタリングによるアプタマー選抜法の開発

    飛田安梨沙, 上野楓, 高尾隼空, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    キャピラリー電気泳動シンポジウム講演要旨集   40th (CD-ROM)   2020

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  • プラズモニック結晶を用いた非染色PCR産物検出デバイスの開発

    古川ふう大, 井上千種, 川崎大輝, 山田大空, 末吉健志, 末吉健志, 久本秀明, 遠藤達郎

    日本分析化学会年会講演要旨集(Web)   69th   2020

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  • キャピラリー分子ふるい電気泳動を利用した低分子標的アプタマー選抜法

    和田将英, 遠藤達郎, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    分析化学討論会講演要旨集(Web)   80th   2020

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  • キャピラリー分子ふるい電気泳動に基づく低分子標的アプタマー選抜法

    和田将英, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    日本分析化学会年会講演要旨集(Web)   69th   2020

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  • キャピラリー分子ふるい電気泳動による低分子標的核酸アプタマー選抜法

    和田将英, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    キャピラリー電気泳動シンポジウム講演要旨集   40th (CD-ROM)   2020

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  • Rapid, simple, sensitive, and multiple enzyme activity assay using a reagent-release capillary-assembled microdevice with double sweeping

    Kenji Sueyoshi, Ryota Sanuki, Tatsuro Endo, Hideaki Hisamoto

    21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017   1291 - 1292   2020

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    © 17CBMS-0001. In this study, we developed a novel microfluidic platform combining reagent-release capillaries and hydrogels (RRCs and RRGs) for multiple enzyme activity assay. Developed RRC-assembled device provided a rapid, simple and multiple enzyme activity assay by only introducing a sample solution by capillary action. The enzyme reaction products were then concentrated on the basis of double sweeping using both the cationic and anionic micelles from the RRGs by applying voltage. As a result, a rapid, simple, sensitive, and simultaneous assay of caspase-3, trypsin, and alkaline phosphatase was successfully realized by the developed device.

  • Imprinted photonic crystal nanocavity for p-spectrometer

    Kenichi Maeno, Shoma Aki, Kazuo Satoh, Shuichi Murakami, Yusuke Sando, Yusuke Kanaoka, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

    21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017   561 - 562   2020

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    © 17CBMS-0001. In this study, novel concept of micrometer-scale spectrometer (P-spectrometer) composed of imprinted photonic crystal nanocavities (PCNs) was proposed. The P-spectrometer has sufficient size to integrate into microfluidic devices and possible to detect light absorption high sensitively. Here we fabricated a PCN as unit cell of P-spectrometer using nanoimprint lithography, and experimentally confirmed for the first time that the fabricated PCN worked as a detection module for 560 nm wavelength.

  • Design of a PDMS microchannel array-based fast and single-step immunoassay principle using hydrophilic polymer-coated graphene oxide possessing multiple functions

    Akihiro Shirai, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017   1297 - 1298   2020

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    © 17CBMS-0001. This paper reports the preparation of the polyethylene glycol-coated graphene oxide (PEG-GO) possessing highly dispersive, fluorescence quenching and size separation functions, and its application to “single-step” immunoassay. The present immunoassay microdevice was fabricated by combining PDMS microchannel arrays separately immobilizing PEG-GO and fluorescently-labelled antibody as soluble coatings. When a sample solution was introduced into the microdevice by capillary action, all the reagents spontaneously mixed, reacted, and free fluorescently-labelled antibody was penetrated into the PEG structure and effectively quenched. Thus the antigen was successfully detected by single-step operation from the change of fluorescence intensity.

  • A “dyed plasticizer” for rapid and highly sensitive sensing based on a plasticized PVC membrane for capillary array-based microanalytical devices

    T. Mizuta, K. Sueyoshi, T. Endo, H. Hisamoto

    21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017   521 - 522   2020

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    © 17CBMS-0001. In this work, we synthesized a new material, “dyed plasticizer”, [P66614]2[NP] which consists of two trihexyltetradecylphospohonium cations and a naphtholphthalein anion, and prepared the plasticized PVC membrane. We systematically investigated the reversibility of response, sensitivity and response time, and demonstrated rapid and highly-sensitive anion sensing based on the coextraction of anions and protons into the bulk of the plasticized PVC membrane. We prepared a microchannel array device combined with plasticized PVC membrane, and Cl- sensing using flat-bed scanner was successfully demonstrated.

  • 色素液体ナノエマルションの創製と高感度イオンセンシングの基礎検討

    牧佳穂, 大石綾太郎, 水田巽, 末吉健志, 遠藤達郎, 久本秀明

    分析化学討論会講演要旨集(Web)   80th   2020

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  • 高精度な細胞外微粒子分級用マイクロ・ナノ流体デバイスの開発

    藤原聡子, 金子紗也, 森川響二朗, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    日本分析化学会年会講演要旨集(Web)   69th   2020

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  • 高感度イオンセンシングを志向した色素液体ナノエマルションの創製

    牧佳穂, 大石綾太郎, 水田巽, 末吉健志, 遠藤達郎, 久本秀明

    日本分析化学会年会講演要旨集(Web)   69th   2020

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  • Evaluation of surface treatment conditions for reduction of non-specific adsorption on polymer-based photonic crystal

    中野萌, 山田大空, 川崎大輝, 末吉健志, 久本秀明, 遠藤達郎

    日本化学会春季年会講演予稿集(CD-ROM)   100th   2020

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  • 電気泳動フィルタリングによる標的分子捕捉とDNA結合・解離を利用した新規アプタマー選抜法の開発

    高尾隼空, 飛田安梨沙, 上野楓, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    キャピラリー電気泳動シンポジウム講演要旨集   40th (CD-ROM)   2020

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  • 蛍光性色素液体を用いた超高感度FRET検出型PVC膜オプトードの開発

    水田巽, 末吉健志, 遠藤達郎, 久本秀明

    分析化学討論会講演要旨集(Web)   80th   2020

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  • 蛍光性色素液体を用いたFRET検出型PVC膜オプトードの開発と超高感度イオンセンシングへの応用

    水田巽, 末吉健志, 遠藤達郎, 久本秀明

    日本分析化学会年会講演要旨集(Web)   69th   2020

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  • ナノ流路内電気二重層厚さ制御に基づくエクソソーム分級

    藤原聡子, 森川響二朗, 遠藤達郎, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    キャピラリー電気泳動シンポジウム講演要旨集   39th   2019

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  • キャピラリー分子ふるい電気泳動による低分子認識アプタマーの高効率選抜法

    和田将英, 遠藤達郎, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    キャピラリー電気泳動シンポジウム講演要旨集   39th   2019

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  • Inkjet Printingを用いた試薬固定に基づく1stepビオチン測定マイクロデバイスの開発

    河相優子, 角田正也, 末吉健志, 遠藤達郎, 久本秀明

    化学とマイクロ・ナノシステム学会研究会講演要旨集   39th   2019

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  • Inkjet Printingを用いた1step競合型イムノアッセイマイクロデバイスの開発

    河相優子, 角田正也, 末吉健志, 遠藤達郎, 久本秀明

    日本分析化学会年会講演要旨集(Web)   68th   2019

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  • 部分的注入アフィニティキャピラリー電気泳動に基づく核酸標的薬剤スクリーニング

    末吉健志, 末吉健志, 光野恵理子, 西野智哉, 遠藤達郎, 遠藤達郎, 久本秀明

    キャピラリー電気泳動シンポジウム講演要旨集   39th   2019

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  • 電気泳動フィルタリングによる酵素濃縮・生成物分離を利用した酵素反応初速度測定

    高尾隼空, 遠藤達郎, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

    キャピラリー電気泳動シンポジウム講演要旨集   39th   2019

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  • "Dyed Plasticizer": Application to rapid and highly sensitive heparin detection based on PDMS microchannel array devices

    Toshiki Nishihata, Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018   2   884 - 885   2018

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    Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved. A plasticized PVC sensor membrane using “dyed plasticizer”[1], where exceptionally high concentration of dye was retained, exhibits rapid and highly-sensitive color change. In this study, we applied a PVC membrane based on “dyed plasticizer” to heparin detection, and prepared the microchannel array integrated with the plasticized PVC membrane. The results presented here demonstrated that our developed micro analytical device was proved to be effective for rapid and highly-sensitive measurement of heparin.

  • Calcium-selective “dyed plasticizer” on PDMS microchip: Rapid and highly sensitive naked eye-based quantitation of calcium ion

    Y. Niwa, T. Mizuta, K. Sueyoshi, T. Endo, H. Hisamoto

    22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018   2   947 - 949   2018

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    Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved. In this work, we developed a PDMS-based microdevice capable of highly sensitive and rapid calcium ion quantitation based on poly(vinylchloride) (PVC) membrane containing newly-developed calcium-selective “dyed plasticizer”. First, we demonstrated rapid and highly-sensitive cation sensing based on cation-proton exchange at aqueous/membrane interface. Finally, we fabricated a PDMS microchip using this new material for the single step and simultaneous data collection of Ca2+ calibration data, and preliminary results for Ca2+ calibration was obtained in the range of 10-4 ~ 10-1M.

  • A multi-dimensional fractionation microdevice based on digital electrophoresis using convex-concave cartridges

    Yuki Uwagawa, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi

    22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018   4   2016 - 2018   2018

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    Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved. Digital electrophoresis microdevices consisting of convex-concave-type cartridges (CCCs) were developed for a multi-dimensional fractionation of proteins. The various CCCs containing a different functionalized hydrogel allow simple connection and detachment of each other, which enables multi-dimensional separation, preconcentration, and fractionation of proteins based on digital electrophoresis using the serially connected hydrogels. As a typical result, two-dimensional fractionation of model proteins based on isoelectric focusing and molecular sieving was successfully achieved by the developed microdevices.

  • 2波長の共振モードによる蛍光増強を指向したハニカム格子フォトニック結晶の設計

    志水友哉, 安藝翔馬, 前野権一, 寺尾京平, 塩見太朗, 末吉健志, 久本秀明, 遠藤達郎, 遠藤達郎

    応用物理学会春季学術講演会講演予稿集(CD-ROM)   65th   2018( ISSN:2436-7613

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  • Functionalized graphene nanoparticle toward rapidly measurable and easy-to-use immunoassay microdevice

    Akihiro Shirai, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018   4   1880 - 1881   2018

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    Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved. Here, we report the newly prepared polyethylene glycol (PEG)-coated graphene nanoparticle (PGN) possessing fluorescence quenching and size separation functions, and its application to single-step immunoassay. The PGN was prepared by the coacervation method, and by optimizing the preparation condition, PGN successfully separated the free fluorescently-labelled antibody and immunocomplex by size difference. By separately adsorbing fluorescently-labelled antibody and PGN onto the inner wall of two PDMS microchannel arrays and combining, the present immunoassay microdevice was prepared. Finally, by introducing a sample solution by capillary action, we successfully measured the C-reactive protein (CRP) as a model antigen within 2 min.

  • 色素分子ドープポリマー製フォトニック結晶ナノ共振器の開発

    前野権一, 安藝翔馬, 寺尾京平, 塩見太朗, 末吉健志, 久本秀明, 遠藤達郎, 遠藤達郎

    応用物理学会春季学術講演会講演予稿集(CD-ROM)   65th   2018( ISSN:2436-7613

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  • Development of hydrogel-supported plasmonic device for label-free biosensor with molecular sieving ability

    山田大空, 川崎大輝, 井上千種, 前野権一, 末吉健志, 久本秀明, 遠藤達郎, 遠藤達郎

    センサ・マイクロマシンと応用システムシンポジウム(CD-ROM)   35th   2018

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  • 低濃度試料測定を指向した鎖状交差プラズモニック結晶構造

    山田大空, 川崎大輝, 前野権一, 安藝翔馬, 末吉健志, 久本秀明, 遠藤達郎, 遠藤達郎

    応用物理学会春季学術講演会講演予稿集(CD-ROM)   65th   2018( ISSN:2436-7613

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  • プラズモニックナノコーンアレイの作製と高感度遺伝子診断デバイスへの応用

    川崎大輝, 山田大空, 西辻凌輔, 前野権一, 安藝翔馬, 末吉健志, 久本秀明, 遠藤達郎, 遠藤達郎

    応用物理学会春季学術講演会講演予稿集(CD-ROM)   65th   2018( ISSN:2436-7613

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  • デザイナブル・デジタル電気泳動デバイスを用いる多次元電気泳動法の開発

    末吉健志, 青木優太, 宇和川悠己, 遠藤達郎, 久本秀明

    分析化学討論会講演要旨集   78th   2018

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  • Evaluation of the relationship between modified surface property of gold nano cone array and its LSPR response toward sensor applications

    川崎大輝, 山田大空, 前野権一, 井上千種, 末吉健志, 久本秀明, 遠藤達郎, 遠藤達郎

    センサ・マイクロマシンと応用システムシンポジウム(CD-ROM)   35th   2018

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  • カートリッジ接続型デバイスを用いたタンパク質のデジタル二次元分画

    宇和川悠己, 遠藤達郎, 久本秀明, 末吉健志

    キャピラリー電気泳動シンポジウム講演要旨集   38th   2018

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  • Position-selective immobilization of sensor membranes on convex-shaped PDMS chip for naked eye-based multiplexed detection

    Tatsumi Mizuta, Kenichi Maeno, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018   2   994 - 995   2018

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    Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved. Herein, a novel method for the intact immobilization of a very thin and soft PVC membrane on a convex-shaped PDMS surface is described. The present method using PET film mask and PVA film as a sacrificial layer allowed successful immobilization of an intact PVC membrane using an ionic liquid-based dye only on the convex-shaped PDMS surface. The present method is applicable for various shapes of PDMS structure without any deformation or increase of the inhomogeneity of PVC membrane.

  • 高選択・高感度センシングを指向したハイドロゲル支持プラズモニックセンサの開発

    山田大空, 川崎大輝, 井上千種, 前野権一, 末吉健志, 久本秀明, 遠藤達郎, 遠藤達郎

    日本バイオマテリアル学会大会予稿集(Web)   40th   2018

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  • TiO₂/ポリマーハイブリッド型フォトニック結晶スラブを用いた高感度蛍光イオンセンサの開発

    安藝 翔馬, 前野 権一, 末吉 健志, 久本 秀明, 遠藤 達郎

    「センサ・マイクロマシンと応用システム」シンポジウム論文集 電気学会センサ・マイクロマシン部門 [編]   34   4p   2017.10

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  • Design and fabrication of room-temperature-curing glass-based photonic crystal waveguide

    34   1 - 4   2017.10

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  • Design and optical characterization of plasmonic crystal for highly sensitive biosensor in visible region

    34   4p   2017.10

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  • Nanoimprinted photonic crystal nanocavity for single cell analysis

    34   4p   2017.10

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  • Fast and single-step fluorescence-based competitive bioassay microdevice combined PDMS microchannel arrays separately immobilizing graphene oxide-analyte conjugates and fluorescently-labelled receptor proteins

    Akihiro Shirai, Kaho Nakashima, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    Analytical Sciences   33 ( 8 )   969 - 972   2017( ISSN:0910-6340 ( eISSN:1348-2246

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    Publishing type:Rapid communication, short report, research note, etc. (scientific journal)  

    © The Japan Society for Analytical Chemistry. In this study, a fast and easy-to-use capillary-type microdevice for competitive bioassay is proposed. The device is composed of polydimethylsiloxane (PDMS) microchannel arrays that separately immobilize polyethylene glycol (PEG) coating, which contained graphene oxide (GO)-analyte conjugates and fluorescently-labelled receptor proteins. The working principle of the device involved the spontaneous dissolution of the PEG coating, subsequent mixing and reaction with analyte to give fluorescence response, triggered by the capillary action-mediated introduction of a sample solution. For principle verification, a competitive biotin assay was successfully demonstrated within 20 s in a single-step operation by detecting the change in fluorescence via microscopy.

    DOI: 10.2116/analsci.33.969

    PubMed

  • 脂質二重膜チャンバーを有するTiO<sub>2</sub>製フォトニック結晶の作製と光学特性評価

    安藝翔馬, 前野権一, 佐藤和郎, 村上修一, 山東悠介, 金岡祐介, 末吉健志, 久本秀明, 遠藤達郎, 遠藤達郎

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)   78th   2017( ISSN:2436-7613

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  • 可視光を用いた高感度センシングを指向したプラズモニック結晶構造の検討

    山田大空, 西口輝一, 前野権一, 安藝翔馬, 久本秀明, 末吉健志, 遠藤達郎, 遠藤達郎

    応用物理学会春季学術講演会講演予稿集(CD-ROM)   64th   2017( ISSN:2436-7613

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  • 可視光での高感度測定を志向した積層構造を有するプラズモニック結晶構造の検討と光学特性評価

    山田大空, 前野権一, 安藝翔馬, 久本秀明, 末吉健志, 遠藤達郎, 遠藤達郎

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)   78th   2017( ISSN:2436-7613

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  • ポリマー製フォトニック結晶ナノ共振器

    前野権一, 安藝翔馬, 佐藤和郎, 村上修一, 山東悠介, 金岡祐介, 末吉健志, 久本秀明, 遠藤達郎, 遠藤達郎

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)   78th   2017( ISSN:2436-7613

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  • ナノインプリント製フォトニック結晶を用いるDNA非標識検出法の検討

    西辻凌輔, 末吉健志, 三宅眞実, 久本秀明, 遠藤達郎, 遠藤達郎

    日本バイオマテリアル学会大会予稿集(Web)   39th   2017

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  • 機能性ヒドロゲルカートリッジ接続によるデジタル電気泳動分析用デザイナブルデバイスの開発

    青木 優太, 末吉 健志, 遠藤 達郎, 久本 秀明

    日本分析化学会講演要旨集   65年会   46 - 46   2016.08

  • Nanoimprinted two-dimensional photonic crystal for detection of fibrinogen using antigen-antibody reaction

    Tatsuro Endo, Kenji Sueyoshi, Hideaki Hisamoto

    MOC 2015 - Technical Digest of 20th Microoptics Conference   2016.02

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    © 2015 The Japan Society of Applied Physics. In this study, for medical diagnostics, the polymer-based two-dimensional photonic crystal (2D-PhC) was fabricated using nanoimprint lithography (NIL)-based nanooptical device fabrication technology that called as "printable photonics". In addition, polymer-based 2D-PhC was applied for detection of fibrinogen which is a biomarker to diagnose the chronic obstructive pulmonary disease (COPD) using antigen-antibody reaction. Based on printable photonics technology, the polymer-based nanooptical device which act in visible region could be fabricated cost effectively and easily. Furthermore, by using our polymer-based 2D-PhC, fibrinogen could detect sensitively and rapidly.

    DOI: 10.1109/MOC.2015.7416439

  • Development of a designable digital electrophoresis device by connecting capillary cartridges filled with a different functionalized hydrogel

    Y. Aoki, T. Kanaoka, K. Matsuda, K. Sueyoshi, T. Endo, H. Hisamoto

    20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016   1448 - 1449   2016

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    To realize a simple, highly sensitive, and "designable" assay of proteins, a novel digital electro-phoresis device is developed by connecting capillary cartridges filled with a different "functionalized hydrogel". The multi-step digital separation based on different electrophoresis modes could be easily demonstrated by designing, arranging, and connecting the capillary cartridges without any complicat-ed fabrications. The model proteins were easily concentrated and separated on the basis of digital molecular sieving electrophoresis by using the developed device, indicating that desirable assays could be realized by the proposed concept.

  • ポリマー/TiO<sub>2</sub>ハイブリッド型フォトニック結晶スラブを用いた蛍光イオンセンシング

    安藝翔馬, 前野権一, 久本秀明, 末吉健志, 遠藤達郎

    応用物理学会秋季学術講演会講演予稿集(CD-ROM)   77th   2016( ISSN:2436-7613

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  • Single-step immunoassay microdevice based on graphene oxide-containing hydrogel possessing fluorescence quenching and size separation functions

    A. Shirai, K. Nakashima, K. Sueyoshi, T. Endo, H. Hisamoto

    20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016   581 - 582   2016

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    This paper reports the preparation and characterization of graphene oxide (GO)-containing hydrogel possessing fluorescence quenching and size separation functions, and its application to single-step immunoassay. Immunoassay microdevice was fabricated by combining PDMS microchannel and GO-containing hydrogel prepared by 2-hydroxyethyl methacrylate (HEMA). Free fluorescently-labeled antibody and immunocomplex was successfully separated by size difference and the former was selectively penetrated into hydrogel to directly adsorb onto bare GO surface for fluorescence quenching-based immunoassay. Here, the concentration of model antigen (human IgG) was successfully measured.

  • DNA-脂質複合体を基材としたナノ光学デバイスの作製

    遠藤 達郎, 末吉 健志, 久本 秀明

    日本バイオマテリアル学会大会予稿集   37回   182 - 182   2015.11

  • ポリマー製フォトニック結晶バイオセンシングデバイス高感度化の検討

    松元 陽子, 遠藤 達郎, 末吉 健志, 久本 秀明

    日本バイオマテリアル学会大会予稿集   37回   314 - 314   2015.11

  • ナノインプリント製フォトニック結晶バイオセンサー用表面修飾剤の合成と特性評価

    神川 楓, 遠藤 達郎, 末吉 健志, 久本 秀明

    日本バイオマテリアル学会大会予稿集   37回   180 - 180   2015.11

  • Double sweeping using reagent-release hydrogels for a highly sensitive electrophoretic bioassay microdevice

    R. Sanuki, K. Sueyoshi, T. Endo, H. Hisamoto

    MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences   1080 - 1082   2015

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    © 15CBMS-0001. This paper reports a newly proposed online sample concentration method named "double sweeping" using hydrogels containing mixed micelles with cationic or anionic charges. Highly effective online concentration of fluorescent molecules typically employed in enzyme activity assays was achieved by the proposed method using the mixed micelles, which overcame the sensitivity problems in previous microdevices and provided up to 200-fold enhancement of the obtained signal. The application of the developed device to the enzyme activity assay was successfully achieved, resulting in the highly sensitive enzyme activity assay with small consumptions of the sample and reagents.

  • Rapid and sensitive enzyme activity assay microdevice by electrophoretic filtration and a hydrogel immobilizing fluorescent substrates

    T. Nishiwaki, S. Miyamoto, K. Sueyoshi, T. Endo, H. Hisamoto

    MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences   939 - 941   2015

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    © 15CBMS-0001. This paper reports a rapid, sensitive, and simple enzyme activity assay device by the combination of electrophoretic filtration of enzymes and detection using a hydrogel immobilizing fluorescent substrates (detection gel). The detection gel and a dense hydrogel to filtrate enzymes were successively prepared in a capillary. The effective filtration of low-concentration enzymes using the dense hydrogel accelerated the enzyme reaction of the fluorescent substrate in the detection gel, providing the rapid response and the enhancement of the fluorescence intensity.

  • Rapid and highly sensitive immunoassay device based on electrophoretic filtration using a hydrogel immobilizing fluorescent substrates

    S. Miyamoto, K. Sueyoshi, T. Endo, H. Hisamoto

    MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences   948 - 950   2015

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    © 15CBMS-0001. This paper reports the development of a rapid and highly sensitive electrophoretic immunoassay device using a hydrogel immobilizing fluorescent substrates. Electrophoretic filtration of immunocomplex using a hydrogel allowed the bound/free separation and sensitive detection. Enzyme reaction of the immobilized substrate was accelerated by electrophoretic filtration of the enzyme-labeled antibody, providing the rapid and sensitive detection. The proposed device using the hydrogel immobilizing fluorescent substrates overcame the problems in conventional immunoassays such as the long analysis time, low sensitivity, and complex procedure.

  • Mass-producible capillary-assembled microchip (cas-chip) for multiple sensing with single-step operation toward point of care testing

    A. Shirai, T. G. Henares, K. Sueyoshi, T. Endo, H. Hisamoto

    MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences   826 - 828   2015

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    © 15CBMS-0001. Here, we report newly fabricated mass-producible capillary-assembled microchip (CAs-CHIP) for multiple bioanalytical sensing with single-step operation that is expected to be utilized in the field of point of care testing (POCT). Today, there are huge number of studies on Micro-Total Analysis Systems toward medical diagnosis, however the usability and portability are still the problems. To improve these, we developed the new CAs-CHIP that is mainly composed of square glass capillaries possessing chemical sensor functions arrayed side-by-side. The integration of various capillary-type sensors would allow us to develop the universal and on-demand microdevices for use in POCT.

  • Highly sensitive enzyme activity assay micro device based on isoelectric focusing using bifunctional fluorescent substratesand reagent-release hydrogels

    K. Sugawara, K. Sueyoshi, T. Endo, H. Hisamoto

    MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences   1987 - 1989   2015

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    © 15CBMS-0001. A microfluidic isoelectric focusing (IEF) device with reagent-release hydrogels (RRGs) were newly developed for a highly sensitive enzyme assay using a two-steps enzyme reaction of bifunctional substrates. Problems in previous assays such as low sensitivity and a difficulty in the separation of an intermediate and a final product of the reaction were solved by the developed IEF device, providing the accurate kinetic assay of the two-steps enzyme reaction with high sensitivity and a simple experimental procedure.

  • UV硬化性PDMSを用いたナノインプリント製二次元フォトニック結晶の作製とセンサー応用

    遠藤 達郎, 梶田 浩志, 末吉 健志, 田中 覚, 久本 秀明

    日本バイオマテリアル学会大会予稿集   36回   243 - 243   2014.11

  • 機能性グラフェン放出キャピラリーを用いた簡便・迅速なサンドイッチ型イムノアッセイデバイスの開発

    白井 亮洋, Terence Henares, 末吉 健志, 遠藤 達郎, 久本 秀明

    日本分析化学会講演要旨集   63年会   27 - 27   2014.09

  • 2D photonic crystal biosensor immobilized with sugar-incorporating nanogelparticles

    Yuhei Terada, Tatsuro Endo, Hirokazu Seto, Hideaki Hisamoto, Yu Hoshino, Yoshiko Miura

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY   248   2014.08( ISSN:0065-7727

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    Publishing type:Research paper, summary (international conference)  

  • Evaluation of the stacked functional hydrogels towards microfluidic western blotting based on multi-demensional digital electrophoresis using capillary-assembled microchip

    K. Matsuda, T. Kanaoka, K. Sueyoshi, T. Endo, H. Hisamoto

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014   2396 - 2398   2014

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    © 14CBMS. This paper reports an evaluation of a separation and online concentration of proteins based on molecular sieving and affinity trapping using stacked functional hydrogels prepared within capillaries towards a development of a novel microfluidic western blotting device based on multi-dimensional digital electrophoresis using a capillary-assembled microchip (CAs-CHIP). To realize the rapid, sensitive, and selective multi-detection of proteins, various capillaries filled with a different functionalized hydrogels were prepared. The prepared capillaries successfully provided the separation and up to 180-fold concentration due to a molecular sieving effect, or selective capturing using antibodies.

  • Ultrafast and single-step immunoassay using functional graphene oxide for capillary-assembled microchip

    Akihiro Shirai, Terence G. Henares, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014   956 - 958   2014

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    © 14CBMS. Here, we report ultrafast and single-step immunoassay within 30 seconds using functional graphene oxide and chemically-modified capillary-type microdevice. Today, there are huge number of studies on bioassay using graphene oxide (GO) which has exceptional fluorescence quenching ability, however there are some problems that are the needs for step-by-step operation and long analysis time depending on the reaction scale. We solved such problems by using capillary-type microdevice immobilizing polyethylene glycol (PEG) coating inside capillary, which contains antibody-conjugated GO and fluorescently-labeled antibody. By using the present device, simply introducing a sample solution by capillary action allowed successful single-step immunoassay.

  • Two-dimensional digital electrophoresis of proteins using mosaic hydrogel

    T. Kanaoka, K. Matsuda, K. Sueyoshi, T. Endo, H. Hisamoto

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014   67 - 69   2014

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    © 14CBMS. We developed two-dimensional digital electrophoresis using a mosaic-like multi-layered hydrogel plate (mosaic hydrogel). In two-dimensional digital electrophoresis, proteins are separated and concentrated nearby the interface of the layered hydrogels on the basis of IEF and molecular sieving by functionalized mosaic hydrogel. The mosaic hydrogels was prepared by photolithographic technique, and it was confirmed that the prepared hydrogel enables a discontinuous separation and on-line concentration of proteins simultaneously. Two-dimensional digital electrophoresis of proteins based on isoelectric focusing and molecular sieving were successfully demonstrated using the developed device integrating the mosaic hydrogel.

  • Rapid and highly sensitive electrophoretic immunoassay device based on the on-line concentration of enzyme-labeled antibody using hydrogel immobilizing fluorescent substrate

    S. Miyamoto, K. Sueyoshi, T. Endo, H. Hisamoto

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014   2423 - 2425   2014

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    © 14CBMS. This paper reports the development of a rapid, highly sensitive and simple electrophoretic immunoassay device using a hydrogel immobilizing fluorescent substrate. To achieve the proposed electrophoretic immunoassay, electrophoretic behavior of enzyme-labeled antibody and subsequent reaction with fluorescent substrate immobilized within hydrogel were investigated. Especially, it was confirmed that a novel detection scheme based on an on-line concentration of enzyme-labeled antibody using the hydrogel could accelerate the enzyme reaction, providing a rapid and highly sensitive detection with a simple procedure.

  • Preparation of hydrogel immobilizing fluorescent substrate on a flexible polymer sheet and its application to mass-producible and single-step multi-sensing device

    S. Odaka, K. Jigawa, S. Funano, T. G. Henares, K. Sueyoshi, T. Endo, H. Hisamoto

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014   947 - 949   2014

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    © 14CBMS. Here, we report the development of functional hydrogel array on a flexible polymer sheet, allowing fabrication of mass-producible and single-step multi-sensing device, which can be used for the discrimination of sick patient serum among many serum samples. In the present study, we designed and synthesized a fluorescent substrate monomer for hydrogen peroxide (H<inf>2</inf>O<inf>2</inf>) which was immobilizable to hydrogel by copolymerization, and preliminary investigation for the development of single-step multi-sensing device for glucose, cholesterol, and uric acid, using this monomer was carried out.

  • POlymer/TIO<inf>2</inf> hybrid two-dimensional photonic crystal for enhanced fluorescence detection

    Shoma Aki, Tatsuro Endo, Kenji Sueyoshi, Hideaki Hisamoto

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014   2291 - 2293   2014

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    © 14CBMS. In this study, we developed polymer/TiO2 hybrid two-dimensional photonic crystal (2D-PhC) optical sensor that has the ability of fluorescence enhancement based on the matching of fluorescence wavelength and confineable wavelength of 2D-PhC for the first time. Ten and three times fluorescence enhancement were achieved for PMMA/TiO2 and plasticized PVC/TiO2 2D-PhCs, respectively.

  • Polymer based photonic crystal cavity for highly sensitive optical detection

    K. Maeno, S. Aki, T. Endo, K. Sueyoshi, H. Hisamoto

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014   2288 - 2290   2014

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    © 14CBMS. In this study, a polymer-based 2-dimensional photonic crystal (PhC) cavity for highly sensitive optical sensor was successfully designed and fabricated. By using this sensor, label-free, highly sensitive, and cost effective biosensor can be realized. We demonstrated introduction of a cavity into polymer-based PhC to confine the visible light (650 nm) into the cavity by the photonic bandgap (PBG) and found that the present device possess single polymer layer detection sensitivity in Layer-by-Layer (LbL) deposition process.

  • Hydrogel based 2D-photonic crystal including acrylic acid for biosensing apprication

    Yoko Matsumoto, Takashi Araki, Tatsuro Endo, Kenji Sueyoshi, Hideaki Hisamoto

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014   2038 - 2040   2014

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    © 14CBMS. In this study, hydrogel-based two-dimensional photonic crystal (2D-PhC) including acrylic acid for biosensing application was successfully developed by using nanoimprint lithography (NIL). This hydrogel-based 2D-PhC enables to reduce non-specific adsorption of proteins and to simply immobilize biorecognition elements such as an antibody onto the 2D-PhC surface via a carboxyl group of acrylic acid. By using this hydrogel-based 2D-PhC, refractive index change was detected as a reflection intensity change, and the detection of antigen-antibody reaction could be achieved.

  • ウロキナーゼ活性センシングを指向したペプチド固定化フォトニック結晶の開発

    橋本 わかな, 末吉 健志, 久本 秀明, 遠藤 達郎

    日本分析化学会講演要旨集   62年会   166 - 166   2013.08

  • 試薬放出キャピラリー/試薬含有ハイドロゲル組み合わせ型マイクロデバイスの開発 糖質分解酵素活性の簡便・高感度アッセイ

    安倉 直希, 末吉 健志, 遠藤 達郎, 久本 秀明

    日本分析化学会講演要旨集   62年会   145 - 145   2013.08

  • 点欠陥二次元フォトニック結晶を用いたラベルフリーDNAセンシング

    上田 知奈, 末吉 健志, 久本 秀明, 遠藤 達郎

    日本分析化学会講演要旨集   62年会   166 - 166   2013.08

  • 新規脂溶性蛍光基質固定化キャピラリーを用いたガン診断マーカーの1ステップイムノアッセイ

    舩野 俊一, 菅原 将人, Henares Terence, 末吉 健志, 遠藤 達郎, 久本 秀明

    日本分析化学会講演要旨集   62年会   377 - 377   2013.08

  • ドライケミストリー組み合わせ型電気泳動マイクロチップの開発 ドライ試薬担持ろ紙積層カートリッジを用いたタンパク検出の基礎検討

    宮原 佑太, 末吉 健志, 遠藤 達郎, 久本 秀明

    日本分析化学会講演要旨集   62年会   257 - 257   2013.08

  • Development of novel label-free enzyme activity assay using nanoimprinted photonic crystal for urokinase activity measurement

    Wakana Hashimoto, Tatsuro Endo, Kenji Sueyoshi, Hideaki Hisamoto

    17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013   2   859 - 861   2013

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    The urokinase (uPA) activity is widely recognized as a potential parameter for diagnosis of breast cancer metastasis. In this study, we developed novel sensing mechanism using peptides-immobilized two dimentional photonic crystal (PhC) for determination of uPA activity. The detection limit of developed device was 80 mIU/mL, and this value is lower than diagnosis concentration of breast cancer metastasis[1].

  • Single-step enzyme immunoassay using lipophilic fluorescent substrate for capillary-assembled microchip

    Masato Sugahara, Shun Ichi Funano, Terence G. Henares, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013   1   497 - 499   2013

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    Here, we report a new principle of single-step enzyme immunoassay by using a newly-synthesized lipophilic fluorescent substrate, which can be immobilized to a plasticized polyvinyl chloride (PVC) coating by hydrophobic interaction. The plasticized PVC was coated inside a square glass capillary and further coated by hydrogel coating as a second layer which worked for discriminating antigen-antibody complex and free antibody. Then soluble coating containing enzyme-labeled antibody was coated as a third layer to successfully prepare a capillary for single-step immunoassay. Introducing a sample solution by capillary action allowed successful single-step immunoassay of cancer marker protein, alphafeto protein (AFP). Copyright © (2013) by the Chemical and Biological Microsystems Society All rights reserved. All rights reserved.

  • Single-step caspase-3 inhibitor assay by using combinable PDMS capillary (CPC) sensor array

    Tadashi Ishimoto, Kaede Jigawa, Terence G. Henares, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013   3   1544 - 1546   2013

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    Caspase-3 inhibitor assay important in drug development was successfully integrated into "Single-Step" by solving the problem of low-activity enzyme immobilization. We developed a combinable PDMS capillary (CPC) sensor for enzyme inhibition assay using the concave- and convex-type PDMS substrates. In the conventional enzyme inhibitor assay, complicated analytical procedures, such as mixing and dissolution, are required. In contrast, this sensor allows single-step assay possible only by sample introduction via capillary action. Total reaction time was reduced from lh to 3-5 min, and simultaneous acquisition of multiple data required to determine IC50 value was successfully achieved by using CPC sensor array.

  • Simple and rapid immunoassay using micro isoelectric focusing device and reagent release hydrogels

    Y. Fujii, K. Sueyoshi, T. Endo, H. Hisamoto

    17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013   2   973 - 975   2013

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    Simple and rapid immunoassay based on micro isoelectric focusing (IEF) was newly developed by using hydrogels containing acid or base as ion suppliers. The prepared hydrogels have enough mechanical strength and can eliminate a handling operation of acid and base solutions which often causes an unfavorable hydrodynamic flow in conventional IEF. As a result, the proposed device provided the easy-to-use reagent release capillary-IEF (RRC-IEF). The RRC-IEF with reagent release hydrogels was also applied to microscale immunoassay, realizing a simple and rapid immunoassay by concentration and separation of the bound/free analytes within 10 min.

  • Simple and highly-sensitive enzyme activity assay microdevice based on the combination of reagent-release hydrogel and capillary array

    Naoki Agura, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013   1   672 - 674   2013

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    This paper reports the development of simple and highly-sensitive enzyme assay microdevice by the combination of reagent-release hydrogel (RRG) and reagent-release capillary (RRC). On-line concentration of fluorescent enzyme reaction products and change of pH condition in RRC were demonstrated by using RRGs containing micellar or basic solution, providing highly-sensitive assay of enzyme activity without a problem of pH mismatching. Copyright © (2013) by the Chemical and Biological Microsystems Society All rights reserved. All rights reserved.

  • Plasticized PVC-based photonic crystal for ion sensing application

    Shoma Aki, Tatsuro Endo, Kenji Sueyoshi, Hideaki Hisamoto

    17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013   2   925 - 927   2013

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    In this study, we developed plasticized poly (vinyl chloride) (PVC) based two-dimensional photonic crystal (2D-PhC) optical sensor for the first time, that has the ability of ion selective extraction. Plasticized PVC is well known as an optical ion sensor material[1]. This novel plasticized PVC based 2D-PhC was fabricated by nanoimprint lithography (NIL). Pattern transfer was confirmed using scanning electron microscopy (SEM). The reflection peak of PVC-based PhC could be observed at 580 nm by diffraction of light. Potassium sensing selective elementswere dissolved in plasticized PVC-based PhC, and successfully observed the K+-selective response by the reflection peak intensity change.

  • Flatbed scanner-based detection for capillary-assembled microchip

    Shohei Kubo, Terence G. Henares, Shun Ichi Funano, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013   1   269 - 271   2013

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    A conventional flatbed scanner was employed for direct detection of colored solution in capillary-based micro analytical device. Since the flatbed scanner is a common and inexpensive detection equipment (usually ∼ 50 USD), establishing the method for detecting colored solution within capillary or microchannel would allow us widespread use of microfluidic devices including point-of-care (POC) testing. Here, scan conditions (resolution, scan direction) were systematically investigated, and determined detection limits of various colored solutions within 100 jam-sized square capillary. Finally, pH sensing was performed as a demonstration and successfully obtained a calibration curve. Copyright © (2013) by the Chemical and Biological Microsystems Society All rights reserved.

  • Fabrication of hydrogel-based two-dimensional photonic crystal for optical sensor application

    Takashi Araki, Tatsuro Endo, Kenji Sueyoshi, Hideaki Hisamoto

    17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013   3   1562 - 1564   2013

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    We fabricated hydrogel-based photonic crystals (PhC) using poly(ethyleneglycol) diacrylate (PEGDA-PhC) which included with tungsten trioxide (WO3) for refractive index sensor applications. Reduction of nonspecific adsorption by using PEGDA as base material and sensitivity enhancement by adding WO3particle were successfully achieved. Moreover, simple functionalization by amino group using allylamine during polymerization was investigated.

  • Capillary sensor array chip as a "sample-to-answer" device for simple, rapid, and multiple component analysis of serum sample

    Yusuke Kimura, Terence G. Henares, Shun Ichi Funano, Tatsuro Endo, Hideaki Hisamoto

    Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012   1384 - 1386   2012

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    This paper reports a novel microdevice showcasing the rapid and simultaneous multi-analyte sensing of different serum components; glucose, cholesterol, pH and alkaline phosphatase (ALP). Discrimination between normal and high analyte concentration in a serum sample was achieved and results were obtained within minutes after sample introduction. The complexity of microdevice fabrication and fluid handling from our previous system, capillary-assembled microchip (CAs-CHIP) [1-2], has been circumvented in this work that led to a simple fabrication and easy operation. Furthermore, we have exploited the fluorescein-based probes for simultaneous multi-analyte sensing by using only one fluorescence filter.

  • "Advances in micro/nano-bioanalysis - from molecular science to device applications-"

    Hideaki Hisamoto Prof, Naoki Hirayama Prof

    Analytical Sciences   28 ( 1 )   1 - 2   2012( ISSN:0910-6340 ( eISSN:1348-2246

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  • ELISA-CIEF using capillary-based microdevice: Highly-sensitive elisa based on capillary-isoelectric focusing of enzyme reaction product

    Yuta Uenoyama, Ken Ikegami, Daniel Citterio, Koji Suzuki, Shun Ichi Funano, Terence G. Henares, Tatsuro Endo, Hideaki Hisamoto

    Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012   1735 - 1737   2012

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    Sensitivity enhancement of enzyme-linked immunosorbent assay (ELISA) in capillary-based microdevice was achieved by the concentration of fluorescent final enzyme reaction product using capillary-isoelectric focusing (CIEF). In order to realize this, fluorescent substrate molecule, which can change its structure into ampholyte ion after an enzyme reaction, was newly synthesized and used. Approximately seven-fold enhancement of sensitivity and 1-2 orders of magnitude lower the detection limit were achieved.

  • Advances in micro/nano-bioanalysis.

    Hideaki Hisamoto, Naoki Hirayama

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry   27 ( 4 )   349   2011( eISSN:1348-2246

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  • Combinable-PDMS capillary (CPC) sensor array as a universal and mass-producible microfluidic platform towards all single-step assay of multiple analytes on a single microfluidic device

    Terence G. Henares, Yosuke Uchiyama, Fumito Okubo, Kozo Akai, Toshio Yao, Hideaki Hisamoto

    Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences   899 - 901   2009

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    In this study, we fabricated the Combinable-PDMS Capillary (CPC) sensor allowing single step assay by simply introducing a sample solution by capillary force. CPC is composed of a convex-shaped PDMS plate immobilizing undissolvable reagents and a concave-shaped PDMS plate immobilizing dissolvable reagents. Since the concave-shaped PDMS has a deeper channel size compared to that of convex structure, combining these PDMS plates allowed easy fabrication of capillary structure possessing different reagents inside the same capillary. This method is useful for immobilizing two different reagents which reacts each other. As an application, we carried out a single-step enzyme inhibitor assay using the CPC. First, a fluorescent substrate is immobilized with Polyethylene glycol (PEG) on concave-shaped PDMS plate. Secondly, trypsin, an enzyme, is quantitatively immobilized using Layer-by-Layer (LbL) technique on convex-shaped PDMS plate. Then these PDMS plates are combined to fabricate CPC for enzyme inhibitor assay. Buffer solution containing various concentrations of leupeptin, a trypsin inhibitor, is introduced into the capillary, then inhibition curve was obtained. © 2009 CBMS.

  • Current development in microfluidic immunosensing chip

    Terence G. Henares, Fumio Mizutani, Hideaki Hisamoto

    Analytica Chimica Acta   611 ( 1 )   17 - 30   2008.03( ISSN:0003-2670

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    Publishing type:Book review, literature introduction, etc.  

    This review accounts for the current development in microfluidic immunosensing chips. The basic knowledge of immunoassay in relation to its microfluidic material substrate, fluid handling and detection mode are briefly discussed. Here, we mainly focused on the surface modification, antibody immobilization, detection, signal enhancement and multiple analyte sensing. Some of the clinically important currently implemented on the microfluidic immunoassay chips are C-reactive protein (CRP), prostate specific antigen (PSA), ferritin, vascular endothelial growth factor (VEGF), myoglobin (Myo), cardiac troponin T (cTnT), cardiac troponin I (cTnI), and creatine kinase-cardiac muscle isoform (CK-MB). The emerging microfludic immunosensor technology may be a promising prospect that can propel the improvement of clinical and medical diagnosis. © 2008 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.aca.2008.01.064

    PubMed

  • Development of single-step heterogenous sandwich capillary immunosensor for capillary-assembled microchip (CAs-CHIP) Integration

    T. G. Henares, H. Yoshimura, T. Yao, H. Hisamoto

    12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference   895 - 897   2008

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    A novel capillary immunosensor capable of single-step heterogeneous sandwich immunoassay for capillary-assembled microchip (CAs-CHIP) integration is presented. It is mainly composed of a covalently attached primary antibody and non-covalently immobilized secondary labeled antibody in the inner wall of the square glass capillary. Sample solution containing antigen enters via capillary force resulting to the release of the secondary labeled antibody and immunocomplex formation occurs on the capillary wall followed by fluorescence detection. Here, the capillary immunosensor contains the fluorescein-labeled and unlabeled anti-human IgG for non-covalent and covalent immobilization, respectively. The immunosensor respond successfully to changes in human IgG concentration. © 2008 CBMS.

  • A capillary-assembled microchip with an electrochemical detection system

    Proceedings of the Chemical Sensor Symposium   43   16 - 18   2007.03

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  • Development of enzyme-release capillary for capillary-assembled microchip towards multiple metabolite sensing

    T. G. Henares, E. Maekawa, F. Mizutani, R. Sekizawa, H. Hisamoto

    Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007   427 - 429   2007

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    © 2007 CBMS. A simple sensing conduit for capillary-assembled microchip (CAs-CHIP) integration that is capable of metabolite detection by fluorescence microscopy is developed. This sensing capillary called enzyme-release capillary (ERC) contains enzyme/s that are adsorbed inside the square glass capillary with the aid of polyethylene glycol acting as scaffold. Metabolite solution enters the conduit via capillary force and the enzyme/s are released leading to the detection of a fluorescent biochemical product. Here, the stability of the E-galactosidase immobilized capillary is observed to last for almost a month. Moreover, the co-immobilized glucose oxidase-peroxidase-thiamine system for glucose measurement showed a detection limit of about 30 PM.

  • “Drop-and-sip⇝ fluid handling technique for reagent-release capillary-based capillary-assembled microchip (cas-chip): Multiple cell lysate sample delivery and caspase-activity sensing

    T. G. Henares, F. Mizutani, R. Sekizawa, H. Hisamoto

    Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007   874 - 876   2007

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    © 2007 CBMS. The basic concept of “drop-and-sip” fluid delivery on a reagent-release capillary-based capillary-assembled microchip (RRC-based CAs-CHIP) has been published elsewhere [1]. Here, we intend to present significant details on the active and passive microfluidics present in the RRC-based CAs-CHIP. More importantly, this fluid handling technique is implemented on a real sample, HeLa cell lysate, wherein chemotheraphy-induced cell death with a certain drug, doxorubicin, is profiled with ten different protease-sensing capillaries embedded on the CAs-CHIP comparing the enzyme activities in a doxorubicin-treated and untreated cell lysates simultaneously.

  • III Chemically-Functionalized Microchip(Analytical Chemistry)

    Hisamoto H., Terabe S.

    Annual review, Graduate School of Material Science and Graduate School of Life Science, University of Hyogo   17   92 - 92   2006.10( ISSN:1349-5011

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  • Microchip Electrophoresis(Analytical Chemistry)

    Matsubara N., Hisamoto H., Terabe S.

    Annual review, Graduate School of Material Science and Graduate School of Life Science, University of Hyogo   16   98 - 98   2005.10( ISSN:1349-5011

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  • Capillary-assembled microchip (CAs-CHIP): A new method for integrating multiple chemical functions onto a single microfluidic device

    H Hisamoto, Y Nakashima, C Kitamura, S Funano, M Yasuoka, K Morishima, Y Kikutani, T Kitamori, S Terabe

    Micro Total Analysis Systems 2004, Vol 1   1 ( 296 )   33 - 35   2005( ISSN:0260-6291

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    A novel concept of assembling various chemical functions onto a single microfluidic device is proposed. The concept, "CAPILLARY-ASSEMBLED MICROCHIP (CAs-CHIP), involves embedding chemically functionalized square capillaries onto a lattice microchannel network fabricated on polydimethyl siloxane (PDMS) possessing same channel dimensions to outer dimensions of square capillaries (Figure 1). This approach would allow easy fabrication of chemically-functionalized microfluidic device by freely embedding functional square capillaries on PDMS microchannel network. Here we report a fabrication method of CAs-CHIP, and an application for preparing a multiple chemical sensing chip as an example.

  • Health care chip based on integrated electrochemical sensors used for clinical diagnostics, bun and creatine

    Chia Hsien Chang, Hiroki Ogawa, Masao Nagai, Akio Oki, Madoka Takai, Hideaki Hisamoto, Yasuhiro Horiike

    Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences   1   1312 - 1314   2005

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    In this study, focus has been placed on potentiometric measurement of ammonia ions for BUN (blood urea nitrogen) and Creatine sensors by using new 19-membered crown ionophore, TD19C6 (3wt%), PVC (30wt%) membranes along with anionic additives (10mol %) K-TCPB and TFPB, and plasticizers (67wt%) BBPA and TOTM. The screen printed electrodes Ag/AgCl (250mm in diameter), newly developed non-liquid junction reference electrode, painless needle and disposable polycarbonate chip (PC), designed of using a trace of amount of whole blood extracted from painless needle is demonstrated. Copyright © 2005 by the Transducer Research Foundation, Inc.

  • Chemical processing on microchips for analysis, synthesis, and bioassay

    Manabu Tokeshi, Yoshikuni Kikutani, Akihide Hibara, Kiichi Sato, Hideaki Hisamoto, Takehiko Kitamori

    Electrophoresis   24 ( 21 )   3583 - 3594   2003.11( ISSN:0173-0835

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    Publishing type:Book review, literature introduction, etc.  

    This review describes our recent research on miniaturization of chemical systems. We have developed a miniaturization methodology based on pressure-driven multiphase laminar flow and a highly sensitive detection tool, the thermal lens microscope. Some representative applications of the methodology in the fields of analysis, synthesis, and bioassay are described.

    DOI: 10.1002/elps.200305661

    PubMed

  • マイクロ膜化学システムの構築 (特集 インテグレーテッド・ケミストリー--最新動向と今後の展望)

    久本 秀明

    ファインケミカル   32 ( 16 )   37 - 42   2003.09( ISSN:0913-6150

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  • Microchip-based chemical and biochemical analysis systems

    Kiichi Sato, Akihide Hibara, Manabu Tokeshi, Hideaki Hisamoto, Takehiko Kitamori

    Advanced Drug Delivery Reviews   55 ( 3 )   379 - 391   2003.02( ISSN:0169-409X

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    Publishing type:Book review, literature introduction, etc.  

    This review focuses on chemical and biochemical analysis systems using pressure-driven microfluidic devices or microchips. Liquid microspace in a microchip has several characteristic features, for example, short diffusion distances, high specific interfacial area and small heat capacity. These characteristics are the key to controlling micro unit operations and constructing new integrated chemical systems. By combining multiphase laminar flow and the micro unit operations, such as mixing, reaction, extraction and separation, continuous flow chemical processing systems are realized in the microchip format. By applying these concepts, several different analysis systems were successfully integrated on a microchip. In this paper, we introduce the microchip-based chemical systems for wet analysis of cobalt ion, multi-ion sensors, immunoassay, and cellular analysis. © 2002 Elsevier Science B.V. All rights reserved.

    DOI: 10.1016/S0169-409X(02)00225-9

    PubMed

  • Integration of chemical and biochemical analysis systems into a glass microchip

    Kiichi Sato, Akihide Hibara, Manabu Tokeshi, Hideaki Hisamoto, Takehiko Kitamori

    Analytical Sciences   19 ( 1 )   15 - 22   2003.01( ISSN:0910-6340

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    Publishing type:Book review, literature introduction, etc.  

    This review focuses on the integration of chemical and biochemical analysis systems into glass microchips for general use. By combining multiphase laminar flow driven by pressure and micro unit operations, such as mixing, reaction, extraction and separation, continuous-flow chemical processing systems can be realized in the microchip format, while the application of electrophoresis-based chip technology is limited. The performances of several analysis systems were greatly improved by microchip integration because of some characteristics of microspace, i.e., a large specific interface area, a short molecular diffusion time, a small heat capacity and so on. By applying these concepts, several different analysis systems, i.e., wet analysis of cobalt ion, multi-ion sensor, immunoassay, and cellular analysis, were successfully integrated on a microchip. These microchip technologies are promising for meeting the future demands of high-throughput chemical processing.

    DOI: 10.2116/analsci.19.15

    PubMed

  • ミクロ空間における分子輸送と溶媒抽出--マイクロ化学システムの鍵

    久本 秀明, 渡慶次 学, 火原 彰秀

    日本イオン交換学会誌   14 ( 1 )   38 - 43   2003.01( ISSN:0915-860X

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  • マイクロチップ抽出場の創製からマイクロチップ化学へ

    渡慶次 学, 火原 彰秀, 久本 秀明, 北森 武彦

    ぶんせき   329   257 - 262   2002.05( ISSN:0386-2178

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  • マイクロチップにおける検出法とマイクロ分析システム (新春企画 近未来テクノロジー"マイクロ化学プラント"の可能性)

    渡慶次 学, 久本 秀明, 北森 武彦

    計装   45 ( 1 )   21 - 24   2002.01( ISSN:0368-5780

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  • マイクロ分析システム

    佐藤 記一, 久本 秀明, 渡慶次 学, 木村 博子, 北森 武彦

    BME : bio medical engineering   15 ( 10 )   24 - 30   2001.10( ISSN:0913-7556

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  • マイクロチップが開くポストゲノムテクノロジー

    北森 武彦, 久本 秀明

    生物物理化学 = Journal of Electrophoresis   45   4 - 4   2001.05( ISSN:0031-9082

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  • インテグレーテッド・ケミストリー - 化学技術のオーダーシフト革命 -

    久本 秀明, 渡慶次 学, 北森 武彦

    化学と工業 = Chemistry and chemical industry   54 ( 5 )   564 - 568   2001.05( ISSN:0022-7684

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  • Chemical system integrated on microchips - Beneficial effects expected for liquid micro space

    Hideaki Hisamoto, Takehiko Kitamori

    Petrotech   23 ( 11 )   924 - 927   2000.11( ISSN:0386-2763

  • PVC waveguide sensor for determination of ion concentration

    Kyungho Kim, Haruyuki Minamitani, Hideaki Hisamoto, Kouji Suzuki, Shinwon Kang

    Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings   ( 1 )   93 - 94   1996( ISSN:0589-1019

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    In this paper, a PVC optical thin-film waveguide sensor is proposed which can select a specific ion and measure its concentration depending on materials added into waveguide membrane. In order to investigate the applicability of this sensor to Ca++ and Na+ measurement, the waveguide was fabricated with poly vinyl chloride (PVC), bis ethyl hexyl phthalate (BEHP), lipophilic anionic dye (LAD-3), and ionophores (K23E1 for Ca++ or C14DD16C5 for Na+). For this purpose, we applied the sensor to sample solution whose CaCl2 concentration was gradually altered. Also, we applied it to NaCl solution. In this experiment, we found that this PVC thin film waveguide possessed good ray propagation at 488 nm of argon laser. In addition, it showed the chemical reaction to ion enough to select it and measure its concentration. These findings suggested that the proposed PVC waveguide ion sensor is applicable to various ions.

  • オプティカルファイバーケミカルセンサー

    久本秀明, 鈴木孝治

    高分子 45   1996

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  • PVC waveguide sensor for measurement of ion concentration

    Kim Kyungho, Minamitani Haruyuki, Hisamoto Hideaki, Suzuki Kouji, Sasaki Keisuke

    Proceedings of the IEICE General Conference   1995 ( 1 )   301 - 301   1995.03

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Presentations

  • ナノインプリント製フォトニック結晶を用いたメチル化シトシン検出 Domestic conference

    中島悠佑,川崎大輝,末吉健志,久本秀明,遠藤達郎

    第70回応用物理学会 春季学術講演会  2023.03 

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    Presentation type:Poster presentation  

  • 蛍光性A–π–D型色素の設計・合成と有機溶媒中水分センシング膜の作製 Domestic conference

    森本あみ,末吉健志,遠藤達郎,久本秀明

    日本化学会 第103春季年会  2023.03 

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    Presentation type:Poster presentation  

  • Selection of Structure-Induced Aptamer Targeting Small Molecules Based on Capillary Sieving Electrophoresis International conference

    Masahide Wada, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi

    RSC Tokyo International Conference 2022  2022.12 

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    Presentation type:Poster presentation  

  • 極限濃度色素液体材料の創製と 高性能化学センシングへの展開 Invited

    久本秀明

    センサ&IoTコンソーシアム公開シンポジウム  2022.11 

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    Presentation type:Public lecture, seminar, tutorial, course, or other speech  

  • イオン選択性色素液体ナノエマルション含有ゲルの開発と1ステップ分析デバイスへの応用 Domestic conference

    小泉有右,末吉健志,遠藤達郎,久本秀明

    化学とマイクロ・ナノシステム学会 第46回研究会   2022.11 

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    Presentation type:Poster presentation  

  • ミクロスケール電気泳動フィルタリングデバイスを用いた高効率アプタマー選抜 Domestic conference

    高尾隼空,上野楓,飛田安梨沙,遠藤達郎,久本秀明,末吉健志

    化学とマイクロ・ナノシステム学会 第46回研究会   2022.11 

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  • フォトニック結晶を用いたDNAメチル化解析 Domestic conference

    中島悠佑,川﨑大輝,末吉健志,久本秀明,遠藤達郎

    化学とマイクロ・ナノシステム学会 第46回研究会   2022.11 

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    Presentation type:Poster presentation  

  • ミクロスケール電気泳動フィルタリングによる異種標的分子混合試料に対するアプタマー同時選抜 Domestic conference

    上野楓,遠藤達郎,久本秀明,末吉健志

    第42回キャピラリー電気泳動シンポジウム   2022.10 

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    Presentation type:Poster presentation  

  • 蛍光性膜溶媒 / 蛍光色素間のFRET機構を利用した高感度カチオンセンシング用蛍光色素液体薄膜の開発 Domestic conference

    西海豪祐,水田巽,末吉健志,遠藤達郎,久本秀明

    日本分析化学会第71年会  2022.09 

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    Presentation type:Poster presentation  

  • 電気泳動フィルタリングデバイスを用いたエクソソームアプタマー選抜・解析 Domestic conference

    末吉健志,飛田安梨沙,上野楓,高尾隼空,遠藤達郎,久本秀明

    日本分析化学会第71年会  2022.09 

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    Presentation type:Oral presentation (general)  

  • キャピラリー分子ふるい電気泳動法による低分子標的・構造誘起型アプタマー選抜 Domestic conference

    和田将英,遠藤達郎,久本秀明,末吉健志

    日本分析化学会第71年会  2022.09 

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    Presentation type:Poster presentation  

  • インクジェットプリンティングによる試薬固定化法を利用する1ステップバイオアッセイマイクロデバイスの開発 Domestic conference

    相優子,白井亮洋,角田正也,井手上公太郎,末吉健志,遠藤達郎,久本秀明

    日本分析化学会第71年会  2022.09 

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    Presentation type:Oral presentation (invited, special)  

  • 高機能センシング材料・多機能集積マイクロ分析デバイスの基盤技術開発研究 Domestic conference

    久本秀明

    日本分析化学会第71年会  2022.09 

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    Presentation type:Oral presentation (invited, special)  

  • ミクロスケール電気泳動フィルタリングに基づく簡便なアプタマー選抜 Domestic conference

    高尾隼空,遠藤達郎,久本秀明,末吉健志

    日本分析化学会第71年会  2022.09 

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    Presentation type:Oral presentation (general)  

  • 化学センサーからマイクロ分析デバイス・システムへ :現在の研究に至るまで

    久本秀明

    九州分析化学若手の会 第35回若手研究講演会および第40回夏季セミナー  2022.07  九州分析化学若手の会・日本分析化学会九州支部

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    Presentation type:Oral presentation (invited, special)  

  • 部分的注入-非平衡アフィニティキャピラリー電気泳動に基づく核酸-低分子間相互作用解析 Domestic conference

    末吉健志,光野恵理子,遠藤達郎,久本秀明

    第29回クロマトグラフィーシンポジウム   2022.06 

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    Presentation type:Oral presentation (general)  

  • キャピラリー分子ふるい電気泳動による低分子標的構造誘起型アプタマー選抜 Domestic conference

    和田将英,遠藤達郎,久本秀明,末吉健志

    第29回クロマトグラフィーシンポジウム   2022.06 

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  • 疎水性極限濃度色素液体材料の開発と 高性能化学センシング Invited

    久本秀明

    中央大学講演会  2022.05 

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  • 高機能センシング材料に基づく 多機能集積マイクロ分析デバイスの 基盤技術開発研究

    久本秀明

    日本女子大学講演会  2022.05 

  • 核酸アプタマー選抜におけるキャピラリー分子ふるい電気泳動に基づく構造特性分離 Domestic conference

    末吉健志,和田将英,遠藤達郎,久本秀明

    日本分析化学会 第82回分析化学討論会   2022.05 

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  • アニオン選択性色素液体ナノエマルションの開発:エマルション内色素-イオノフォア間相互作用による応答性能向上 Domestic conference

    牧佳穂,大石綾太郎,水田巽,末吉健志,遠藤達郎,久本秀明

    日本分析化学会 第82回分析化学討論会   2022.05 

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  • 酵素基質・色素液体に基づく酵素応答性オプトードの基礎検討 Domestic conference

    和田奈由子,末吉健志,遠藤達郎,久本秀明

    日本分析化学会 第82回分析化学討論会   2022.05 

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  • マイクロ・ナノ流路内電気二重層厚さ調節に基づくナノ粒子分級用デバイスの開発 Domestic conference

    阿部友哉,藤原聡子,森川響二朗,遠藤達郎,久本秀明,末吉健志

    化学とマイクロ・ナノシステム学会 第45回研究会  2022.05 

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  • プラズモニック/フォトニック複合構造体による高感度DNA解析 Domestic conference

    川﨑大輝,末吉健志,久本秀明,遠藤達郎

    化学とマイクロ・ナノシステム学会 第45回研究会  2022.05 

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  • ミクロスケール電気泳動フィルタリングデバイスを用いた1ラウンドアプタマー選抜 Domestic conference

    末吉健志,高尾隼空,飛田安梨沙,上野楓,遠藤達郎,久本秀明

    化学とマイクロ・ナノシステム学会 第45回研究会  2022.05 

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    Presentation type:Oral presentation (general)  

  • TiO2フォトニック結晶を用いたエンドトキシン検出法の開発 Domestic conference

    西辻凌輔,植田渉太郎,末吉健志,久本秀明,遠藤達郎

    化学とマイクロ・ナノシステム学会 第45回研究会  2022.05 

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  • 広範囲増強電場を有するプラズモニックナノコーンアレイの作製とSERSセンサへの展開 Domestic conference

    藤原聡子,川﨑大輝,末吉健志,久本秀明,遠藤達郎

    化学とマイクロ・ナノシステム学会 第45回研究会  2022.05 

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  • Quantum dots / TiO2 ハイブリット型フォトニック結晶の作製及び可視域応答バイオセンサへの応用 Domestic conference

    植田渉太郎,川﨑大輝,西辻凌輔,山田大空,末吉健志,久本秀明,遠藤達郎

    第69回応用物理学会春季学術講演会  2022.03 

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  • プラズモニック結晶を用いた非標識PCR法の開発 Domestic conference

    古川渢大,山田大空,川崎大輝,西辻凌輔,遠藤達郎,末吉健志,久本秀明

    第69回応用物理学会春季学術講演会  2022.03 

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  • フォトニック結晶を用いた細胞代謝産物の非標識検出 Domestic conference

    王彦康,中島悠佑,植田渉太郎,末吉健志,久本秀明,遠藤達郎

    第69回応用物理学会春季学術講演会  2022.03 

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  • ミクロスケール電気泳動フィルタリングデバイスを用いた核酸アプタマー選抜 Invited Domestic conference

    末吉健志,高尾隼空,飛田安梨沙,上野楓,遠藤達郎,久本秀明

    日本化学会 第102春季年会   2022.03 

  • Quantum Dots / TiO2 Hybrid Photonic Crystal: Fabrication and Characterization for Optical Biosensor Application in Visible Region International conference

    Shotaro Ueda, Daiki Kawasaki, Hirotaka Yamada, Ryosuke Nishitsuji, Kenji Sueyoshi, Hideaki Hisamoto, Tasuro Endo

    RSC Tokyo International Conference 2021  2021.12 

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  • Development of highly sensitive and selective plasticized PVC membrane optical sensor based on silver-responsive ionic liquid based-dye International conference

    Shuto Oka, Kenji Sueyoshi , Tatsuro Endo, Hideaki Hisamoto

    RSC Tokyo International Conference 2021  2021.12 

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  • Highly-sensitive cation sensing optodes based on FRET-enhanced fluorescence using fluorescent dyed plasticizer International conference

    Kosuke Nishiumi , Tatsumi Mizuta, Kenji Sueyoshi , Tatsuro Endo, Hideaki Hisamoto

    RSC Tokyo International Conference 2021  2021.12 

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    Presentation type:Poster presentation  

  • キャピラリー分子ふるい電気泳動によるランダム配列DNAの構造安定性に基づく分離 Domestic conference

    和田将英,遠藤達郎,久本秀明,末吉健志

    第41回キャピラリー電気泳動シンポジウム   2021.12 

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    Presentation type:Poster presentation  

  • 部分的注入アフィニティキャピラリー電気泳動による四重鎖核酸-低分子間相互作用解析 Domestic conference

    光野恵理子,遠藤達郎,久本秀明,末吉健志

    第41回キャピラリー電気泳動シンポジウム   2021.12 

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    Presentation type:Poster presentation  

  • ミクロスケール電気泳動フィルタリングによるエクソソームアプタマー選抜 Domestic conference

    飛田安梨沙,上野楓,高尾隼空,遠藤達郎,久本秀明,末吉健志

    第41回キャピラリー電気泳動シンポジウム   2021.12 

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    Presentation type:Poster presentation  

  • キャピラリー分子ふるい電気泳動に基づく低分子標的構造誘起型アプタマー選抜法 Domestic conference

    和田将英,遠藤達郎,久本秀明,末吉健志

    第32回クロマトグラフィー科学会議  2021.11 

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    Presentation type:Poster presentation  

  • ヒドロゲル部分充填キャピラリーデバイスを用いた高効率アプタマー電気泳動選抜法の開発 Domestic conference

    末吉健志,高尾隼空,飛田安梨沙,上野楓,遠藤達郎,久本秀明

    第32回クロマトグラフィー科学会議  2021.11 

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    Presentation type:Poster presentation  

  • 高感度イオン検出を志向した色素液体ナノエマルションの開発とマイクロ分析デバイスへの応用 Domestic conference

    牧佳穂,末吉健志,遠藤達郎,久本秀明

    化学とマイクロ・ナノシステム学会 第44回研究会  2021.11 

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    Presentation type:Poster presentation  

  • ミクロスケール電気泳動フィルタリングデバイスを用いた核酸アプタマー選抜 Domestic conference

    高尾隼空,飛田安梨沙,上野楓,遠藤達郎,久本秀明,末吉健志

    化学とマイクロ・ナノシステム学会 第44回研究会  2021.11 

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    Presentation type:Poster presentation  

  • ポリマー製フォトニック結晶シートを用いたスマートフォンによるSERS-CoV-2スパイクタンパク質の高感度検出 Domestic conference

    川崎大輝,山田大空,末吉健志,久本秀明,遠藤達郎

    化学とマイクロ・ナノシステム学会 第44回研究会  2021.11 

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    Presentation type:Poster presentation  

  • イオン選択性可塑化PVC複合型プラズモニックイオンセンサの開発 Domestic conference

    遠藤達郎,井上千種,川崎大輝,山田大空,末吉健志,久本秀明

    第38回「センサ・マイクロマシンと応用システム」シンポジウム   2021.11 

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    Presentation type:Poster presentation  

  • FRET型蛍光色素液体ナノエマルションの応答機構の基礎検討 Domestic conference

    小泉有右,水田巽,末吉健志,遠藤達郎,久本秀明

    日本分析化学会第70年会  2021.10 

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    Presentation type:Poster presentation  

  • IONIC LIQUID-BASED DYE (IL-DYE) NANOEMULSION (NE) AS A HIGH-SENSITIVITY ION SENSING COMPONENT OF MICRO ANALYTICAL DEVICES International conference

    Kaho Maki, Kenji Sueyoshi, Tatsuro Endo, and Hideaki Hisamoto

    The 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021)  2021.10 

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    Presentation type:Poster presentation  

  • ミクロスケール電気泳動フィルタリングに基づくアプタマー選抜法の基礎評価・高効率化 Domestic conference

    上野楓,高尾隼空,飛田安梨沙,遠藤達郎,久本秀明,末吉健志

    第11回CSJ化学フェスタ2021  2021.09 

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    Presentation type:Poster presentation  

  • 固定化不要な標的分子捕捉および高効率なDNA結合を利用したアプタマーの電気泳動選抜法 Domestic conference

    高尾隼空,飛田安梨沙,上野楓,久本秀明,遠藤達郎,末吉健志

    日本分析化学会第70年会  2021.09 

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    Presentation type:Poster presentation  

  • キャピラリー分子ふるい電気泳動に基づく低分子標的構造誘起型アプタマー選抜法 Domestic conference

    末吉健志,和田将英,遠藤達郎,久本秀明

    日本分析化学会第70年会  2021.09 

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  • 銀イオン応答性色素液体の開発と高感度・高選択的オプティカルセンサーへの応用 Domestic conference

    岡嵩人,末吉健志,遠藤達郎,久本秀明

    日本分析化学会第70年会  2021.09 

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  • キャピラリー分子ふるい電気泳動に基づく低分子標的アプタマー選抜のための構造形成型/非形成型核酸ライブラリの構築 Domestic conference

    末吉健志,和田将英,遠藤達郎,久本秀明

    第 28 回クロマトグラフィーシンポジウム  2021.06 

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    Presentation type:Poster presentation  

  • アプタマー選抜のための安定構造形成型・非形成型DNAライブラリーの調製 Domestic conference

    和田将英,遠藤達郎,久本秀明,末吉健志

    日本核酸医薬学会 第6回年会   2021.06 

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    Presentation type:Poster presentation  

  • ミクロスケール電気泳動フィルタリングによるアプタマー選抜法の開発 Domestic conference

    飛田安梨沙,上野楓,高尾隼空,遠藤達郎,久本秀明,末吉健志

    日本核酸医薬学会 第6回年会   2021.06 

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    Presentation type:Poster presentation  

  • 低分子標的アプタマー選抜のためのキャピラリー分子ふるい電気泳動 Domestic conference

    和田将英,遠藤達郎,久本秀明,末吉健志

    日本分析化学会 第81回分析化学討論会  2021.05 

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    Presentation type:Poster presentation  

  • 多段階部分的注入アフィニティキャピラリー電気泳動に基づく核酸-低分子化合物間相互作用解析 Domestic conference

    光野恵理子,遠藤達郎,久本秀明,末吉健志

    日本分析化学会 第81回分析化学討論会  2021.05 

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  • FRET機構を利用した超高感度カチオンセンシング用蛍光色素液体薄膜の開発 Domestic conference

    西海豪祐,水田巽,末吉健志,遠藤達郎,久本秀明

    日本分析化学会 第81回分析化学討論会  2021.05 

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    Presentation type:Poster presentation  

  • ミクロスケール電気泳動フィルタリングによる高効率アプタマー選抜法の開発 Domestic conference

    上野楓,高尾隼空,飛田安梨沙,遠藤達郎,久本秀明,末吉健志

    日本分析化学会 第81回分析化学討論会  2021.05 

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  • フォトニック結晶を用いたDNAメチル化の光検出 Domestic conference

    中島悠佑,小林奈緒,植田渉太郎,川崎大輝,末吉健志,久本秀明,遠藤達郎

    化学とマイクロ・ナノシステム学会 第43回研究会  2021.05 

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    Presentation type:Poster presentation  

  • 疎水性極限濃度色素液体材料に基づく高性能 化学センシング系の設計 Invited Domestic conference

    久本秀明

    日本化学会 第101春季年会 特別企画 | 分析化学と材料化学・界面化学の融合による新学問 領域の提案  2021.03 

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    Presentation type:Oral presentation (invited, special)  

  • An enzyme-responsive fluorescent ionic liquid-based nanosensor toward highly sensitive biosensing International conference

    Ryoutarou Oishi, Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    RSC Tokyo International Conference 2020  2020.12 

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    Presentation type:Poster presentation  

  • Size sorting of exosomes by tuning the thicknesses of the electric double layers in nanochannels International conference

    Satoko Fujiwara, Kyojiro Morikawa, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi

    RSC Tokyo International Conference 2020  2020.12 

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    Presentation type:Poster presentation  

  • Preparation of ionic liquid-based dye (IL-Dye) nanoemulsion (NE) for high-sensitivity ion sensing International conference

    Kaho Maki, Ryoutarou Oishi, Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

    RSC Tokyo International Conference 2020  2020.12 

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    Presentation type:Poster presentation  

  • 電気泳動フィルタリングに基づく酵素反応初速度直接測定法の開発

    高尾 隼空, 遠藤 達郎, 久本 秀明, 末吉 健志

    日本分析化学会講演要旨集  2019.08  (公社)日本分析化学会

  • 簡便・迅速・高感度な1stepELISAへの応用を指向した酵素応答性疎水性色素液体の開発

    大石 綾太郎, 水田 巽, 末吉 健志, 遠藤 達郎, 久本 秀明

    日本分析化学会講演要旨集  2019.08  (公社)日本分析化学会

  • キャピラリー分子ふるい電気泳動に基づく低分子標的アプタマー選抜法の開発

    和田 将英, 遠藤 達郎, 久本 秀明, 末吉 健志

    日本分析化学会講演要旨集  2019.08  (公社)日本分析化学会

  • Inkjet Printingを用いた1 step競合型イムノアッセイマイクロデバイスの開発

    河相 優子, 角田 正也, 末吉 健志, 遠藤 達郎, 久本 秀明

    日本分析化学会講演要旨集  2019.08  (公社)日本分析化学会

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Industrial Property Rights

  • 液状色素を含む組成物、膜、その製造方法、化学センサー、液状色素、液状色素の製造方法、簡易検査用(創薬および/または診断分析)デバイスまたはキット、並びに薄膜発光材料

    久本 秀明

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    property_type:Patent 

    Application no:再表2018/151311 

    Announcement no:WO2018/151311 

    Patent/Registration no:7127834 

Outline of collaborative research (seeds)

  • Development of Chemical Sensors and Biosensors

Acceptance of Researcher

  • 2022  Number of researchers:1

Charge of on-campus class subject

  • Advanced Applied Chemistry III

    2021    

  • Advanced Applied Chemistry I

    2021    

  • Special Topics in Applied Chemistry II

    2021    

  • Analytical Chemistry A

    2021    

  • Introduction to Materials, Chemistry and Chemical Engineering

    2021    

  • Instrumental Methods of Analysis

    2021    

  • Advanced Analytical Chemistry in Technology

    2021    

  • Selected Topics in Industrial Analytical Chemistry

    2021    

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Faculty development activities

  • FDセミナー「大阪公立大学におけるFDのあり方について考える(1)」に参加  2022

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    「大阪公立大学におけるFDのあり方について考える(1)」に参加した。(2022年11月4日)

  • 「工学FDセミナー『教育改善のための、ポートフォリオシステム「てぃら・みす」活用法』」に参加  2022

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    「工学FDセミナー『教育改善のための、ポートフォリオシステム「てぃら・みす」活用法』」に参加した。(2022年9月2日)

  • 全学FDセミナー「feedback studioとwebクリッカー 教育をよりインタラクティブにする2つのツールの活用法」に参加  2022

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    全学FDセミナー「feedback studioとwebクリッカー 教育をよりインタラクティブにする2つのツールの活用法」に参加。(2022年7月8日)

  • 2021年度工学FDセミナー「「自分でできる国際研究広報」〜SNSを用いたミクロ研究広報のすすめ〜」に参加  2021

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    9/1

  • 2021年度FDセミナー「THEランキングを通して授業カリキュラム、授業実践を振り返る」に参加  2021

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    11/1

  • 2020年度FDセミナー「反転授業実践セミナー」に参加  2020

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    FDセミナー「反転授業実践セミナー」
    日時:2021年 3月15日(月)14:00~16:30
       【第1部】14:00~15:00
        講師 森澤正之氏(山梨大学 大学院総合研究部 工学域 教授)
       【第2部】15:30~16:30
        講師 鹿住大助氏(島根大学 教育・学生支援機構 大学教育センター 准教授)

  • 2020年度第2回工学全体FDセミナーに参加  2020

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    2020年度第2回工学全体FDセミナー
    「研究室指導に必要なコーチング技能入門WS
    -学生が自己効力感を高める工夫-」

    日時:9月7日(月)13:30-15:30
    場所:Zoomによるオンライン開催 

  • FDセミナー「ルーブリック評価入門」に参加  2019

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Number of papers published by graduate students

  • 2022

    Number of graduate students presentations:10

Number of instructed thesis, researches

  • 2022

    Number of instructed the graduation thesis:Number of graduation thesis reviews:7

    [Number of instructed the Master's Program] (previous term):[Number of instructed the Master's Program] (letter term):5

    [Number of master's thesis reviews] (chief):[Number of master's thesis reviews] (vice-chief):10

    [Number of doctoral thesis reviews] (chief):[Number of doctoral thesis reviews] (vice-chief):1

Social Activities

  • RSC Tokyo Editorial and Advisory Board Member Summit

    Role(s): Panelist

    2023.03

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    Type:Seminar, workshop

    Number of participants:13(人)

  • 大阪府立生野高校「SSH探究Ⅱ成果発表会」の見学と指導助言・運営指導委員会出席

    Role(s): Consultant, Logistic support

    大阪府立生野高校  2022.09 - Now

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    Type:Cooperation with government and educational institutions

    Number of participants:1(人)

Academic Activities

  • Sensing and Bio-Sensing Research(1報)

    Role(s): Peer review

    2023.01

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    Type:Scientific advice/Review 

  • Analytical Chemistry(9報)

    Role(s): Peer review

    2022.04 - 2023.03

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    Type:Scientific advice/Review 

  • Sensors and Actuators B: Chemical(6報)

    Role(s): Peer review

    2022.04 - 2023.03

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    Type:Scientific advice/Review 

  • Sensors & Diagnostics(3報)

    Role(s): Peer review

    2022.04 - 2023.03

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    Type:Scientific advice/Review 

  • Lab on a Chip (5報)

    Role(s): Peer review

    2022.04 - 2023.03

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    Type:Scientific advice/Review 

  • Biosensors and Bioelectronics(3報)

    Role(s): Peer review

    2022.04 - 2023.03

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    Type:Scientific advice/Review 

  • Analyst(3報)

    Role(s): Peer review

    2022.04 - 2023.03

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    Type:Scientific advice/Review 

  • Journal of Analysis and Testing(1報)

    Role(s): Peer review

    2022.04

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    Type:Scientific advice/Review 

  • ACS Biomaterials Science & Engineering(1報)

    Role(s): Peer review

    2022.04

  • Analytical Chemistry

    2021.08 - Now

  • Analyst

    2021.08 - Now

  • Materials Research Bulletin

    2021.06

  • Chemical Engineering Journal

    2021.05 - 2021.06

  • Sensors and Actuators B

    2021.04 - 2021.10

  • Journal of Materials Chemistry B

    2021.04

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