Updated on 2024/04/08

写真a

 
Tezuka Takahiro
 
Organization
Graduate School of Agriculture Department of Agricultural Biology Associate Professor
School of Agriculture Department of Agricultural Biology
Title
Associate Professor
Affiliation
Institute of Agriculture
Contact information
メールアドレス
Affiliation campus
Nakamozu Campus

Position

  • Graduate School of Agriculture Department of Agricultural Biology 

    Associate Professor  2024.04 - Now

  • Graduate School of Agriculture Department of Agricultural Biology 

    Lecturer  2022.04 - 2024.03

  • School of Agriculture Department of Agricultural Biology 

    Associate Professor  2024.04 - Now

  • School of Agriculture Department of Agricultural Biology 

    Lecturer  2022.04 - 2024.03

Degree

  • 博士(農学) ( Tokyo University of Agriculture and Technology )

  • 修士(農学) ( Ibaraki University )

  • 学士(農学) ( Ibaraki University )

Research Areas

  • Environmental Science/Agriculture Science / Science in plant genetics and breeding

  • Environmental Science/Agriculture Science / Horticultural science

  • Life Science / Biodiversity and systematics

  • Life Science / Genetics

Research Interests

  • Breeding barriers

  • Hybrid seed lethality (Seed abortion)

  • Genetic resource

  • Wild species

  • DNA marker

  • Interploidy cross

  • Plant breeding

  • Reproductive isolation

  • Interspecific cross

  • Wide hybridization

  • Hybrid weakness

  • Hybrid lethality

  • Pepper

  • Tobacco

Research subject summary

  • 植物の遠縁交雑育種と生殖隔離に関する研究

  • 植物の種間交雑で認められる雑種致死・雑種弱勢に関する研究

  • 植物の種間・倍数体間交雑における種子発育不全に関する研究

Research Career

  • Research on wide hybridization and reproductive isolation in plants

    Wide hybridization, Reproductive isolation, Cultivated species, Wild species, Diversity  Individual

    2009 - Now 

  • Research on hybrid lethality observed in interspecific crosses in plants

    Hybrid lethality (hybrid necrosis), Hybrid weakness, Interspecific hybridization, Programmed cell death  Individual

    2001 - Now 

  • Research on seed abortion in interploidy-interspecific crosses in plants

    Seed abortion  Individual

    2009 - Now 

  • 解析対象の植物種

    タバコ属、トウガラシ属、イネ、その他  Individual

    2009 - Now 

Professional Memberships

  • Japanese Society of Breeding

    2006.04 - Now   Domestic

  • The Japanese Society for Horticultural Science

    2014.01 - Now

  • Japanese Society for Plant Biotechnology

    2011.01 - Now   Domestic

  • 近畿作物・育種研究会

    2008.04 - Now   Domestic

Committee Memberships (off-campus)

  • Review Editor for Plant Breeding   Frontiers in Plant Science  

    2023.08 - Now 

  • Guest Associate Editor   Frontiers in Plant Science  

    2022.09 - 2023.07 

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    Research Topic: Molecular Insights in Plant Reproductive Isolation Barriers

  • 第39回日本植物バイオテクノロジー学会(堺)大会実行委員   日本植物バイオテクノロジー学会  

    2022.09 

  • 日本農薬学会第45回大会組織委員会委員   日本農薬学会  

    2020.03 

  • シンポジウム委員   近畿作物・育種研究会  

    2016.04 - Now 

  • シンポジウム委員   近畿作物・育種研究会  

    2008.04 - 2012.03 

▼display all

Job Career (off-campus)

  • Osaka Metropolitan University   Graduate School of Agriculture, Department of Agricultural Biology   Lecturer

    2022.04 - Now

  • Osaka Prefecture University   Graduate School of Life and Environmental Sciences   Lecturer

    2015.04 - Now

  • Osaka Prefecture University   Graduate School of Life and Environmental Sciences   Assistant professor

    2008.04 - 2015.03

  • Plant Biotechnology Institute, Ibaraki Agricultural Center   Postdoctoral researcher

    2006.04 - 2008.03

Education

  • Tokyo University of Agriculture and Technology   Graduate School, Division of Agricltural Sciences   Doctor's Course   Graduated/Completed

    2003.04 - 2006.03

  • Ibaraki University   Graduate School, Division of Agriculture   Master's Course   Graduated/Completed

    2001.04 - 2003.03

  • Ibaraki University   Faculty of Agriculture   Bachelor's Course   Graduated/Completed

    1997.04 - 2001.03

Papers

  • Genetic cause of hybrid lethality observed in reciprocal interspecific crosses between Nicotiana simulans and N. tabacum Reviewed

    Takahiro Tezuka, Shota Nagai, Chihiro Matsuo, Toshiaki Okamori, Takahiro Iizuka, Wataru Marubashi

    International Journal of Molecular Sciences   25 ( 2 )   1226   2024.01( ISSN:16616596

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    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    DOI: 10.3390/ijms25021226

    PubMed

  • Editorial: Molecular insights in plant reproductive isolation barriers Reviewed

    Masanobu Mino, Clément Lafon Placette, Takahiro Tezuka

    Frontiers in Plant Science   14   1257823   2023.07( ISSN:1664-462X

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    DOI: 10.3389/fpls.2023.1257823

    PubMed

  • Understanding and overcoming hybrid lethality in seed and seedling stages as barriers to hybridization and gene flow Reviewed

    Hai He, Kumpei Shiragaki, Takahiro Tezuka

    Frontiers in Plant Science   14   1219417   2023.07( ISSN:1664-462X

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Hybrid lethality is a type of reproductive isolation barrier observed in two developmental stages, hybrid embryos (hybrid seeds) and hybrid seedlings. Hybrid lethality has been reported in many plant species and limits distant hybridization breeding including interspecific and intergeneric hybridization, which increases genetic diversity and contributes to produce new germplasm for agricultural purposes. Recent studies have provided molecular and genetic evidence suggesting that underlying causes of hybrid lethality involve epistatic interaction of one or more loci, as hypothesized by the Bateson–Dobzhansky–Muller model, and effective ploidy or endosperm balance number. In this review, we focus on the similarities and differences between hybrid seed lethality and hybrid seedling lethality, as well as methods of recovering seed/seedling activity to circumvent hybrid lethality. Current knowledge summarized in our article will provides new insights into the mechanisms of hybrid lethality and effective methods for circumventing hybrid lethality.

    DOI: 10.3389/fpls.2023.1219417

    PubMed

  • Capsicum annuum with causal allele of hybrid weakness is prevalent in Asia Reviewed

    Kumpei Shiragaki, Shonosuke Seko, Shuji Yokoi, Takahiro Tezuka

    PLoS ONE   17 ( 7 )   e0271091   2022.07

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Reproductive isolation, including hybrid weakness, plays an important role in the formation of species. Hybrid weakness in Capsicum, the cessation of plant growth, is caused by two complementary dominant genes, A from C. chinense or C. frutescens and B from C. annuum. In the present study, we surveyed whether 94 C. annuum accessions had B or b alleles by crossing with C. chinense having the A allele. Of the 94 C. annuum accessions, five had the B allele, three of which were native to Latin America and two were native to Asia. When combined with previous studies, the percentage of B carriers was 41% in Japan, 13% in Asia excluding Japan, 6% in Latin America, and 0% in Europe and Africa. In addition, 48 accessions of C. annuum from various countries were subjected to SSR analysis. Clades with high percentages of B-carriers were formed in the phylogenetic trees. In the principal coordinate analysis, most B-carriers were localized in a single group, although the group also included b-carriers. Based on these results, we presumed that the B allele was acquired in some C. annuum lines in Latin America, and B-carriers were introduced to the world during the Age of Discovery, as along with the b-carriers.

    DOI: 10.1371/journal.pone.0271091

    PubMed

  • Parental Genome Imbalance Causes Hybrid Seed Lethality as Well as Ovary Abscission in Interspecific and Interploidy Crosses in Nicotiana Reviewed

    Hai He, Kumi Sadahisa, Shuji Yokoi, Takahiro Tezuka

    Frontiers in Plant Science   13   899206   2022.05( ISSN:1664-462X

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Enhanced ovary abscission after pollination and hybrid seed lethality result in post-zygotic reproductive isolation in plant interspecific crosses. However, the connection between these barriers remains unclear. Here, we report that an imbalance in parental genomes or endosperm balance number (EBN) causes hybrid seed lethality and ovary abscission in both interspecific and intraspecific-interploidy crosses in the genus Nicotiana. Auxin treatment suppressed ovary abscission, but not hybrid seed lethality, in an interspecific cross between Nicotiana suaveolens and N. tabacum, suggesting that ovary abscission-related genes are located downstream of those involved in hybrid seed lethality. We performed interploidy crosses among N. suaveolens tetraploids, octoploids, and neopolyploids and revealed hybrid seed lethality and ovary abscission in interploid crosses. Furthermore, a higher maternal EBN than paternal EBN caused these barriers, as previously observed in N. suaveolens × N. tabacum crosses. Altogether, these results suggest that maternal excess of EBN causes hybrid seed lethality, which in turn leads to ovary abscission through the same mechanism in both interspecific and interploidy crosses.

    DOI: 10.3389/fpls.2022.899206

    PubMed

  • The hybrid lethality of interspecific F1 hybrids of Nicotiana: a clue to understanding hybrid inviability—a major obstacle to wide hybridization and introgression breeding of plants Reviewed

    Masanobu Mino, Takahiro Tezuka, Sachiko Shomura

    Molecular Breeding   42 ( 2 )   10   2022.02( ISSN:1380-3743

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    DOI: 10.1007/s11032-022-01279-8

    PubMed

    Other URL: https://link.springer.com/article/10.1007/s11032-022-01279-8/fulltext.html

  • Evaluation of crossability between Nicotiana benthamiana and Nicotiana excelsior Reviewed

    Takahiro Tezuka, Naoto Kitamura, Masanori Yanase, Toshinobu Morikawa

    Agronomy   11 ( 12 )   2583 - 2583   2021.12( ISSN:2073-4395

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    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    <jats:p>Wild tobacco species in the Nicotiana section Suaveolentes are promising genetic resources to introduce their disease resistance to cultivated tobacco, Nicotiana tabacum. However, hybrid lethality is observed in hybrid seedlings from crosses between most Suaveolentes species and N. tabacum. In particular, N. benthamiana belonging to the section Suaveolentes produces only viable hybrids after crossing with N. tabacum. In the present study, crossability between N. benthamiana and N. excelsior (section Suaveolentes) was investigated to test the possible usefulness of N. benthamiana as the bridge parent to transfer desirable genes of N. excelsior to N. tabacum via bridge crossing. After reciprocal crosses using three accessions of N. benthamiana and N. excelsior each, several crossing barriers such as cross-incompatibility, seed abortion, and male and female hybrid sterility were observed. Although reciprocal hybrids between N. benthamiana and N. excelsior showed a high degree of chromosome pairing in meiosis, univalents and multivalents, as well as chromosome bridges and lagging chromosomes, were observed. These meiotic abnormalities were thought to cause hybrid sterility. The possible usefulness of reciprocal hybrids between N. benthamiana and N. excelsior is discussed.</jats:p>

    DOI: 10.3390/agronomy11122583

  • Temperature-dependent sugar accumulation in interspecific Capsicum F1 plants showing hybrid weakness Reviewed

    Kumpei Shiragaki, Hajime Furukawa, Shuji Yokoi, Takahiro Tezuka

    Journal of Plant Research   134 ( 6 )   1199 - 1211   2021.11( ISSN:0918-9440

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    In plants, F1 hybrids showing hybrid weakness exhibit weaker growth than their parents. The phenotypes of hybrid weakness are often suppressed at certain temperatures. However, it is unclear whether hybrid weakness in Capsicum annuum × C. chinense is temperature-dependent or not. Our study showed that Capsicum hybrid weakness was suppressed at 30 and 35 °C and was induced at 15, 20, and 25 °C. Moreover, we investigated the time course of hybrid weakness in cell death, metabolite content, and gene expression in leaves of plants transferred to 20 °C after growing at 30 °C for 21 days. The expression of pathogen defense-related genes was upregulated at 1 day after transfer to 20 °C (DAT). Cell death was detected at 7 DAT, plant growth had almost stopped since 14 DAT, and sugars were accumulated at 42 DAT in hybrid plants. The study revealed that some sugar transporter genes, which had been upregulated since 7 DAT, were involved in sugar accumulation in Capsicum hybrid weakness. Thus, our results demonstrated that gene expression changes occur first, followed by physiological and morphological changes after induction of hybrid weakness. These responses observed in this study in Capsicum hybrid weakness are likely to be owed to plant defense responses-like reactions.

    DOI: 10.1007/s10265-021-01340-1

    PubMed

    Other URL: https://link.springer.com/article/10.1007/s10265-021-01340-1/fulltext.html

  • Genetic mapping of the HLA1 locus causing hybrid lethality in Nicotiana interspecific hybrids Reviewed

    Takahiro Tezuka, Naoto Kitamura, Sae Imagawa, Akira Hasegawa, Kumpei Shiragaki, Hai He, Masanori Yanase, Yoshiyuki Ogata, Toshinobu Morikawa, Shuji Yokoi

    Plants   10 ( 10 )   2062   2021.09( ISSN:2223-7747

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    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Hybrid lethality, a postzygotic mechanism of reproductive isolation, is a phenomenon that causes the death of F1 hybrid seedlings. Hybrid lethality is generally caused by the epistatic interaction of two or more loci. In the genus Nicotiana, N. debneyi has the dominant allele Hla1-1 at the HLA1 locus that causes hybrid lethality in F1 hybrid seedlings by interaction with N. tabacum allele(s). Here, we mapped the HLA1 locus using the F2 population segregating for the Hla1-1 allele derived from the interspecific cross between N. debneyi and N. fragrans. To map HLA1, several DNA markers including random amplified polymorphic DNA, amplified fragment length polymorphism, and simple sequence repeat markers, were used. Additionally, DNA markers were developed based on disease resistance gene homologs identified from the genome sequence of N. benthamiana. Linkage analysis revealed that HLA1 was located between two cleaved amplified polymorphic sequence markers Nb14-CAPS and NbRGH1-CAPS at a distance of 10.8 and 10.9 cM, respectively. The distance between these markers was equivalent to a 682 kb interval in the genome sequence of N. benthamiana.

    DOI: 10.3390/plants10102062

    PubMed

  • Two Nicotiana occidentalis accessions enable gene identification for Type II hybrid lethality by the cross to N. sylvestris Reviewed

    Kenji Kawaguchi, Yuichiro Ohya, Maho Maekawa, Takahiro Iizuka, Akira Hasegawa, Kumpei Shiragaki, Hai He, Masayuki Oda, Toshinobu Morikawa, Shuji Yokoi, Takahiro Tezuka

    Scientific Reports   11 ( 1 )   17093   2021.08

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    <title>Abstract</title>Hybrid lethality, meaning the death of F<sub>1</sub> hybrid seedlings, has been observed in many plant species, including <italic>Nicotiana</italic>. Previously, we have revealed that hybrids of the selected <italic>Nicotiana occidentalis</italic> accession and <italic>N. tabacum</italic>, an allotetraploid with S and T genomes, exhibited lethality characterized by the fading of shoot color. The lethality was suggested to be controlled by alleles of loci on the S and T genomes derived from <italic>N. sylvestris</italic> and <italic>N. tomentosiformis</italic>, respectively. Here, we extended the analysis of hybrid lethality using other two accessions of <italic>N. occidentalis</italic> identified from the five tested accessions. The two accessions were crossed with <italic>N. tabacum</italic> and its two progenitors, <italic>N. sylvestris</italic> and <italic>N. tomentosiformis</italic>. After crosses with <italic>N. tabacum</italic>, the two <italic>N. occidentalis</italic> accessions yielded inviable hybrid seedlings whose lethality was characterized by the fading of shoot color, but only the T genome of <italic>N. tabacum</italic> was responsible for hybrid lethality. Genetic analysis indicated that first-mentioned <italic>N. occidentalis</italic> accession carries a single gene causing hybrid lethality by allelic interaction with the S genome.

    DOI: 10.1038/s41598-021-96482-6

    PubMed

    Other URL: https://www.nature.com/articles/s41598-021-96482-6

  • The role of chaperone complex HSP90-SGT1-RAR1 as the associated machinery for hybrid inviability between Nicotiana gossei Domin and N. tabacum L. Reviewed

    Yushi Katsuyama, Mizuho Doi, Sachi Shioya, Sanae Hane, Momoko Yoshioka, Shuichi Date, Chika Miyahara, Tomomichi Ogawa, Ryo Takada, Hanako Okumura, Rie Ikusawa, Sakihito Kitajima, Kenji Oda, Kenji Sato, Yoshikazu Tanaka, Takahiro Tezuka, Masanobu Mino

    Gene   776   145443   2021.04( ISSN:0378-1119

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Two cultured cell lines (GTH4 and GTH4S) of a Nicotiana interspecific F1 hybrid (N. gossei × N. tabacum) were comparatively analyzed to find genetic factors related to hybrid inviability. Both cell lines proliferated at 37 °C, but after shifting to 26 °C, GTH4 started to die similar to the F1 hybrid seedlings, whereas GTH4S survived. As cell death requires de novo expression of genes and proteins, we compared expressed protein profiles between the two cell lines, and found that NgSGT1, a cochaperone of the chaperone complex (HSP90-SGT1-RAR1), was expressed in GTH4 but not in GTH4S. Agrobacterium-mediated transient expression of NgSGT1, but not NtSGT1, induced cell death in leaves of N. tabacum, suggesting its possible role in hybrid inviability. Cell death in N. tabacum was also induced by transient expression of NgRAR1, but not NtRAR1. In contrast, transient expression of any parental combinations of three components revealed that NgRAR1 promoted cell death, whereas NtRAR1 suppressed it in N. tabacum. A specific inhibitor of HSP90, geldanamycin, inhibited the progression of hypersensitive response-like cell death in GTH4 and leaf tissue after agroinfiltration. The present study suggested that components of the chaperone complex are involved in the inviability of Nicotiana interspecific hybrid.

    DOI: 10.1016/j.gene.2021.145443

    PubMed

  • Phylogenetic analysis and molecular diversity of Capsicum based on rDNA-ITS region Reviewed

    Kumpei Shiragaki, Shuji Yokoi, Takahiro Tezuka

    Horticulturae   6 ( 4 )   87 - 13   2020.11

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    The genus Capsicum is comprised of 5 domesticated and more than 30 wild species. The region of nuclear ribosomal DNA internal transcribed spacers (rDNA-ITS) has widely been used for species identification, but has rarely been used in Capsicum. In this study, the evaluation of genetic diversity and a phylogenetic analysis were conducted using rDNA-ITS of 28 Capsicum accessions, including five domesticated and two wild species. We surveyed six conventional keys of domesticated species and another five traits in Capsicum accessions. Specific morphological characteristics were found in C. annuum, C. baccatum, and C. pubescens. Three subclones of each accession were sequenced, and rDNA-ITS polymorphisms were detected in all accessions excluding C. annuum, suggesting that incomplete concerted evolution occurred in rDNA-ITS of Capsicum. The genetic diversity was evaluated using nucleotide polymorphism and diversity. C. annuum had the lowest genetic diversity of all species in this study. The phylogenetic tree formed a species-specific clade for C. annuum, C. baccatum, and C. pubescens. The C. chinense clade existed in the C. frutescens clade, implying that it was a cultivated variant of C. frutescens. C. chacoense likely belonged to the C. baccatum complex according to its morphologic and genetic features. This study indicated that the rDNA-ITS region can be used for simple identification of domesticated Capsicum species.

    DOI: 10.3390/horticulturae6040087

  • Phenylalanine ammonia-lyase and phenolic compounds are related to hybrid lethality in the cross Nicotiana suaveolens × N. tabacum Reviewed

    Kumpei Shiragaki, Rie Nakamura, Shigeki Nomura, Hai He, Tetsuya Yamada, Wataru Marubashi, Masayuki Oda, Takahiro Tezuka

    PLANT BIOTECHNOLOGY   37 ( 3 )   327 - 333   2020.09( ISSN:1342-4580

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Hybrid lethality observed in hybrid seedlings between Nicotiana suaveolens and N. tabacum is characterized by browning, initially of the hypocotyls and eventually of entire seedlings. We investigated the mechanism underlying this browning of tissues. A phenylalanine ammonia-lyase (PAL) gene codes an enzyme involved in a pathway producing phenolic compounds related to the browning of plant tissues. The expression of PAL rapidly increased with the induction of hybrid lethality. Phenolic compounds were observed to be accumulated in whole parts of hybrid seedlings. Treatment of hybrid seedlings with L-2-aminooxy-3-phenylpropionic acid (AOPP), an inhibitor for PAL, suppressed browning and decreased the phenolic content of hybrid seedlings. Although programmed cell death (PCD) was involved in hybrid lethality, AOPP treatment also suppressed cell death and enhanced the growth of hybrid seedlings. These results indicated that PAL is involved in hybrid lethality, and phenolic compounds could be the cause of hybrid lethality-associated tissue browning.

    DOI: 10.5511/plantbiotechnology.20.0606a

    PubMed

  • A hypersensitive response-like reaction is involved in hybrid weakness in F1 plants of the cross Capsicum annuum × Capsicum chinense. Reviewed

    Kumpei Shiragaki, Shuji Yokoi, Takahiro Tezuka

    Breeding science   70 ( 4 )   430 - 437   2020.09( ISSN:1344-7610

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Hybrid weakness in Capsicum is characterized by the termination of leaf differentiation after the development of several leaves. F1 plants in some crosses between Capsicum annuum and Capsicum chinense show weakness; this phenomenon has not been investigated in detail since first reported. In the present study, we characterized morphologically and physiologically hybrid weakness in Capsicum. F1 plants did not show weaker growth than their parents 20 days after germination (DAG), but at 40 DAG, the hybrid weakness phenotype was evidenced by almost complete arrest of new leaf formation, delayed increase in plant height, and reduced upper internode length. The shoot apical meristem (SAM) of F1 plants exhibited delayed development and an abnormal structure characterized by a flat shape and the presence of fuzzy cell layers on the surface. These abnormal SAMs of F1 plants may lead to dwarfism. Dead cells and accumulation of H2O2 were visually detected in leaves of F1 plants, and cell death was considered to be programmed, as it was accompanied by internucleosomal fragmentation of DNA. The expression of immunity marker genes PR1 and PR2 was upregulated in leaves of F1 plants. These results suggest that a hypersensitive response-like reaction is involved in Capsicum hybrid weakness.

    DOI: 10.1270/jsbbs.19137

    PubMed

  • A high maternal genome excess causes severe seed abortion leading to ovary abscission in Nicotiana interploidy-interspecific crosses Reviewed

    Hai He, Shuji Yokoi, Takahiro Tezuka

    Plant Direct   4 ( 8 )   e00257   2020.08( ISSN:2475-4455

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Seed abortion and ovary abscission, two types of postzygotic reproductive barriers, are often observed in interspecific and/or interploidy crosses in plants. However, the mechanisms underlying these reproductive barriers remain unclear. Here, we show that the distinct types of seed developmental abnormalities (type I and type II seed abortion) occur in a phased manner as maternal to paternal genome dosage increases and that type II seed abortion is followed by ovary abscission. We revealed that these two types of seed developmental abnormalities are observed during seed development in the interploidy-interspecific crosses of Nicotiana suaveolens and N. tabacum. Moreover, in the cross showing type II seed abortion, several events, such as changes in abscission-related gene expression and lignin deposition, occurred in the ovary abscission zone, eventually leading to ovary abscission. Notably, successive increases in maternal ploidy using ploidy manipulated lines resulted in successive type I and type II seed abortions, and the latter was accompanied by ovary abscission. Conversely, both types of seed abortion and ovary abscission could be overcome with a ploidy manipulation technique that balances parental ploidy levels. We thus concluded that a high maternal genome excess cross may cause severe seed developmental defects and ovary abscission. Based on our findings, we propose a model explaining the abortion phenomena, where an interaction between the promotive and inhibitive effects of the parental genomes determines the developmental destiny of seeds. Significance statement: We demonstrate that a stepwise increase in maternal ploidy results in a stepwise increase in seed abortion severity, leading to ovary abscission in plants. We propose a model explaining the abortion phenomena, where an interaction between the promotive and inhibitive effects of the parental genomes determines the developmental destiny of seeds.

    DOI: 10.1002/pld3.257

    PubMed

    Other URL: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/pld3.257

  • Morphological changes during juvenile-to-adult phase transition in sorghum Reviewed

    Shumpei Hashimoto, Takahiro Tezuka, Shuji Yokoi

    Planta   250 ( 5 )   1557 - 1566   2019.11( ISSN:0032-0935

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Main conclusion: Morphological and genetic markers indicate that in sorghum, the juvenile-to-adult phase transition occurs during the fourth and fifth leaf stages. This timing differs from those reported for other plants. Abstract: The juvenile-to-adult (JA) phase transition is an important event for optimizing vegetative growth and reproductive success in plants. Among the Poaceae crops, which are a vital food source for humans, studies of the JA phase transition have been restricted to rice and maize. We studied the morphological and genetic changes that occur during the early development of sorghum and found that dramatic changes occur in shoot architecture during the early vegetative stages. Changes were observed in leaf size, leaf shape, numbers of trichomes, and size of the shoot apical meristem. In particular, the length/width ratios of the leaf blades in the fifth and upper leaves were completely different from those of the second to fourth leaves. The fifth and upper leaves have trichomes on their adaxial sides, which were absent on the lower leaves. We also analyzed expression of two microRNAs that are known to be molecular markers of the JA phase transition and found that expression of miR156 was highest in the second to fourth leaves and then was gradually down-regulated, whereas miR172 expression followed the opposite pattern. These results suggest that in sorghum, the second and third leaves represent the juvenile phase, the fourth and fifth leaves are in the transition stage, and the sixth and upper leaves are in the adult phase. Thus, the JA phase transition occurs during the fourth and fifth leaf stages. These findings are expected to be useful for understanding the early development of sorghum.

    DOI: 10.1007/s00425-019-03251-x

    PubMed

  • HWA1- and HWA2-mediated hybrid weakness in rice involves cell death, reactive oxygen species accumulation, and disease resistance-related gene upregulation Reviewed

    Kumpei Shiragaki, Takahiro Iizuka, Katsuyuki Ichitani, Tsutomu Kuboyama, Toshinobu Morikawa, Masayuki Oda, Takahiro Tezuka

    Plants   8 ( 11 )   450   2019.10( ISSN:2223-7747

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    <jats:p>Hybrid weakness is a type of reproductive isolation in which F1 hybrids of normal parents exhibit weaker growth characteristics than their parents. F1 hybrid of the Oryza sativa Indian cultivars ‘P.T.B.7′ and ‘A.D.T.14′ exhibits hybrid weakness that is associated with the HWA1 and HWA2 loci. Accordingly, the aim of the present study was to analyze the hybrid weakness phenotype of the ‘P.T.B.7′ × ‘A.D.T.14′ hybrids. The height and tiller number of the F1 hybrid were lower than those of either parent, and F1 hybrid also exhibited leaf yellowing that was not observed in either parent. In addition, the present study demonstrates that SPAD values, an index correlated with chlorophyll content, are effective for evaluating the progression of hybrid weakness that is associated with the HWA1 and HWA2 loci because it accurately reflects degree of leaf yellowing. Both cell death and H2O2, a reactive oxygen species, were detected in the yellowing leaves of the F1 hybrid. Furthermore, disease resistance-related genes were upregulated in the yellowing leaves of the F1 hybrids, whereas photosynthesis-related genes tended to be downregulated. These results suggest that the hybrid weakness associated with the HWA1 and HWA2 loci involves hypersensitive response-like mechanisms.</jats:p>

    DOI: 10.3390/plants8110450

    PubMed

  • Nicotiana suaveolens accessions with different ploidy levels exhibit different reproductive isolation mechanisms in interspecific crosses with Nicotiana tabacum Reviewed

    Hai He, Takahiro Iizuka, Maho Maekawa, Kumi Sadahisa, Toshinobu Morikawa, Masanori Yanase, Shuji Yokoi, Masayuki Oda, Takahiro Tezuka

    Journal of Plant Research   132 ( 4 )   461 - 471   2019.07( ISSN:0918-9440

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Reproductive isolation, including prezygotic and postzygotic barriers, is a mechanism that separates species. Many species in the Nicotiana section Suaveolentes exhibit reproductive isolation in crosses with Nicotiana tabacum. In this study, we investigated whether the chromosome numbers and ploidy levels of eight Nicotiana suaveolens accessions are related to the reproductive isolation after crosses with N. tabacum by flow cytometry and chromosome analyses. Additionally, the internal transcribed spacer (ITS) regions of the eight N. suaveolens accessions were sequenced and compared with the previously reported sequences of 22 Suaveolentes species to elucidate the phylogenetic relationships in the section Suaveolentes. We revealed that four N. suaveolens accessions comprised 64 chromosomes, while the other four accessions carried 32 chromosomes. Depending on the ploidy levels of N. suaveolens, several types of reproductive isolation were observed after crosses with N. tabacum, including decreases in the number of capsules and the germination rates of hybrid seeds, as well as hybrid lethality and abscission of enlarged ovaries at 12–17 days after pollination. A phylogenetic analysis involving ITS sequences divided the eight N. suaveolens accessions into three distinct clades. Based on the results, we confirmed that N. suaveolens accessions vary regarding ploidy levels and reproductive isolation mechanisms in crosses with N. tabacum. These accessions will be very useful for revealing and characterizing the reproductive isolation mechanisms in interspecific crosses and their relationships with ploidy levels.

    DOI: 10.1007/s10265-019-01114-w

    PubMed

  • Toward the elucidation of reproductive isolation mechanisms in plants: diversity and universality

    Takahiro Tezuka, Katsuyuki Ichitani, Yuichi Matsumoto, Hai He, Tetsu Kinoshita, Shigeo Takumi, Tsutomu Kuboyama

    Breeding Research   21 ( 1 )   75 - 80   2019.06( ISSN:1344-7629

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    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)  

    DOI: 10.1270/jsbbr.21.W05

    J-GLOBAL

  • Storage of plug seedlings of tomato under limited fertilization, and growth, flowering and yield after planting Reviewed

    Hideyuki Tanaka, Koji Murai, Takao Nakanishi, Takahiro Tezuka, Shuji Shiozaki, Masayuki Oda

    The Journal of Horticultural Science and Biotechnology   93 ( 6 )   652 - 658   2018.11( ISSN:14620316

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    DOI: 10.1080/14620316.2018.1439408

  • Morphological variation, growth, and yield of tomato plants vegetatively propagated by the complete decapitation method Reviewed

    Masahumi Johkan, Masaki Ono, Hideyuki Tanaka, Hajime Furukawa, Takahiro Tezuka, Masayuki Oda

    International Journal of Vegetable Science   22 ( 1 )   58 - 65   2016.01( ISSN:1931-5260

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    DOI: 10.1080/19315260.2014.937022

    J-GLOBAL

  • Characterization of circadian rhythms through a bioluminescence reporter assay in Lactuca sativa L. Reviewed

    Takanobu Higashi, Akiko Kamitamari, Nobuya Okamura, Kazuya Ukai, Kenichi Okamura, Takahiro Tezuka, Hirokazu Fukuda

    Environmental Control in Biology   52 ( 1 )   21 - 27   2014( ISSN:1880554X

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Circadian rhythms are observed in many physiological events in plant, and clock genes orchestrate the rhythms of expression of many genes. Precise environmental control of circadian rhythms provides a key technology for enhancing plant growth in artificial environments. In this study, we investigated the basic properties of a circadian rhythm for establishment of its control engineering in lettuce (<i>Lactuca sativa</i> L.). Bioluminescence of transgenic lettuce carrying an <i>AtCCA1::LUC</i> construct as a reporter of circadian gene expression was measured in young lettuce seedlings. We observed three basic properties; free-running circadian rhythms under constant conditions without day-night cycles, entrainment to red and blue light cycles with 12 h light-12 h dark period, and temperature compensation of a free-running period in three lettuce cultivars (Cisco, Cos, Greenwave). In addition, a light-quality dependence of the free-running period and sensitivity to blue-light cycles with small amplitude (20% of variance in average light intensity) and non-24 h period were also observed. The results in this study indicated that it is possible to control lettuce circadian rhythms by non-24 h period light cycles in LED illumination. It will play an important role in the research of control engineering for the circadian clock of lettuce in closed plant factories with artificial lighting.

    DOI: 10.2525/ecb.52.21

    CiNii Article

    Other URL: https://jlc.jst.go.jp/DN/JALC/10032836331?from=CiNii

  • Intact roots promote shoot regeneration from hypocotyl independent of exogenous plant growth regulators in eggplant in vitro Reviewed

    Hideyuki Tanaka, Masahumi Johkan, Kazuhiko Mitsukuri, Takahiro Tezuka, Hajime Furukawa, Masayuki Oda

    Plant Root   7   5 - 11   2013.07( ISSN:1881-6754

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Eggplant (<I>Solanum melongena</I>) seedlings cultured <I>in vitro</I> were excised at the center of the hypocotyl to generate decapitated seedlings with intact roots. This modification of the complete decapitation method (CDM) developed <I>in vivo</I> by Harada et al. (2005) allowed <I>in vitro</I> culture (CDM <I>in vitro</I>; CDM<I>i</I>). As controls, rootless hypocotyl segment explants (approximately 1 cm) and cotyledon explants were cultured on media supplemented with 4.4 μM 6-benzyladenin (BA) and 0.2 μM thidiazuron (TDZ), respectively. Cotyledon explants had formed calli 2 weeks after excision but did not develop adventitious buds, despite the use of optimal conditions reported previously for a different eggplant cultivar. Calli formed at the cut ends of hypocotyls 1 week after excision in both CDM<I>i</I> and hypocotyl cultures, and adventitious buds regenerated 1 week earlier in CDM<I>i</I>. Six weeks after excision, CDM<I>i</I> yielded 11.4 adventitious buds per explant, but only 4.1 formed in hypocotyl culture. Moreover, shoots longer than 1 cm developed 2 weeks earlier in CDM<I>i</I> than in hypocotyl culture. The number of shoots per explant was 8.1 in CDM<I>i</I>, but only 2.4 in hypocotyl culture 6 weeks after cutting. All shoots that developed were rooted on MS medium in CDM<I>i</I>, but only 71% of shoots formed roots in hypocotyl culture. These results indicate that intact roots are important for explant shoot regeneration and development, and CDM<I>i</I> is a simple and efficient method for obtaining multiple shoots without the need to determine optimal concentrations of plant growth regulators and overcome inhibition of rooting in the obtained shoots.

    DOI: 10.3117/plantroot.7.5

    CiNii Article

  • Tumor formation in hybrids between Solanum lycopersicum and S. habrochaites Reviewed

    Takahiro Tezuka, Hidenobu Okinaka, Masayuki Oda

    African Journal of Biotechnology   12 ( 15 )   1745 - 1753   2013.04( ISSN:1684-5315

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    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    DOI: 10.5897/AJB12.1812

  • Factors affecting seed germination of Ilex latifolia and I. rotunda Reviewed

    Takahiro Tezuka, Hisa Yokoyama, Hideyuki Tanaka, Shuji Shiozaki, Masayuki Oda

    HortScience   48 ( 3 )   352 - 356   2013.03( ISSN:00185345

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    DOI: 10.21273/HORTSCI.48.3.352

  • L-2-aminooxy-3-phenylpropionic acid (AOPP) promoting shoot formation from hypocotyl explants of eggplant Reviewed

    Hideyuki Tanaka, Haruka Ueda, Kazuhiko Mitsukuri, Takahiro Tezuka, Shuji Shiozaki, Masayuki Oda

    Research Journal of BioTechnology   8 ( 2 )   4 - 8   2013.02

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

  • Seed and embryo germination in Ardisia crenata Reviewed

    Takahiro Tezuka, Hisa Yokoyama, Hideyuki Tanaka, Shuji Shiozaki, Masayuki Oda

    Journal of Botany   2012   679765   2012.12( ISSN:2090-0120

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    <jats:p><jats:italic>Ardisia crenata</jats:italic> is an evergreen shrub with attractive bright red berries. Although this species is usually propagated by seed, the seeds take a long time to germinate with conventional sowing methods. We investigated the germination capacity of seeds and embryos collected in different months and the effects of seed storage conditions, germination temperature, water permeability of the seed coat, and the endosperm on seed germination. Seeds and embryos collected in late September or later showed good germination rates. Seeds germinated more rapidly after longer periods of storage at low temperature (approximately 5°C), and those stored in dry conditions showed lower emergence frequency than those stored in wet conditions. Seeds germinated at 15–30°C, but not at 5–10°C. Removal of the seed coat enhanced water uptake and seed germination. Seeds with various proportions of the removed seed coat were sown on a medium supplemented with sucrose. The germination frequency increased as the size of the remaining endosperm decreased. However, the opposite results were obtained when seeds were sown on a medium without sucrose. We concluded that the optimal temperature of 25°C is the most critical factor for seed germination in <jats:italic>A. crenata</jats:italic>.</jats:p>

    DOI: 10.1155/2012/679765

  • Adventitious bud formation promoted by auxin treatment in explants from the apical portion of leaves in Begonia × tuberhybrida Reviewed

    Hideyuki Tanaka, Shuji Shiozaki, Satoshi Yamasaki, Kazuhiko Mitsukuri, Yukiko Shimada, Takahiro Tezuka, Masayuki Oda

    Environment Control in Biology   50 ( 4 )   319 - 327   2012.12( ISSN:1880-554X

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    An <i>in vivo</i> method to promote adventitious bud formation in leaf apex explants of <i>Begonia</i>×<i>tuberhybrida</i> was developed based on exogenous treatment with BA, IAA and NAA. Endogenous IAA concentrations of the leaf blade were highest in the apical portion, followed by the middle and basal portions. Treatment with 0.5 mg L<sup>−1</sup> BA increased the percentage of adventitious bud formation to 15% from 0% at 0 mg L<sup>−1</sup> BA, but treatment with BA at concentrations higher than 0.5 mg L<sup>−1</sup> had no additional promotive effect. Adventitious bud formation was increased to 53% by treatment with 0.1 mg L<sup>−1</sup> IAA and 0.5 mg L<sup>−1</sup> BA, and inhibited by 1.0 and 5.0 mg L<sup>−1</sup> IAA. The optimal duration of treatment with 0.1 mg L<sup>−1</sup> IAA was 2–8 days after embedding. No explants formed adventitious buds when treated with fresh 0.1 mg L<sup>−1</sup> IAA at 2-day intervals until 16 days after initial embedding. Similar responses were observed in explants treated with 0.1 mg L<sup>−1</sup> NAA. These results suggested that temporary IAA treatment at 0.1 mg L<sup>−1</sup> during the initial period of embedding is an efficient <i>in vivo</i> method to induce adventitious bud formation in leaf apex explants in <i>B.×tuberhybrida</i>.

    DOI: 10.2525/ecb.50.319

    CiNii Article

  • Nicotiana debneyi has a single dominant gene causing hybrid lethality in crosses with N. tabacum Reviewed

    Takahiro Iizuka, Tsutomu Kuboyama, Wataru Marubashi, Masayuki Oda, Takahiro Tezuka

    Euphytica   186 ( 2 )   321 - 328   2012.07( ISSN:0014-2336

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    Authorship:Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    DOI: 10.1007/s10681-011-0570-3

    CiNii Article

  • Identification of Nicotiana tabacum linkage group corresponding to the Q chromosome gene(s) involved in hybrid lethality Reviewed

    Takahiro Tezuka, Chihiro Matsuo, Takahiro Iizuka, Masayuki Oda, Wataru Marubashi

    PLoS ONE   7 ( 5 )   e37822   2012.05

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    DOI: 10.1371/journal.pone.0037822

    PubMed

    CiNii Article

  • Genes in S and T subgenomes are responsible for hybrid lethality in interspecific hybrids between Nicotiana tabacum and Nicotiana occidentalis Reviewed

    Takahiro Tezuka, Wataru Marubashi

    PLoS ONE   7 ( 4 )   e36204   2012.04

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    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    DOI: 10.1371/journal.pone.0036204

    PubMed

    CiNii Article

  • Effects of auxin and cytokinin on in vivo adventitious shoot regeneration from decapitated tomato plants Reviewed

    Takahiro Tezuka, Masashi Harada, Masahumi Johkan, Satoshi Yamasaki, Hideyuki Tanaka, Masayuki Oda

    HortScience   46 ( 12 )   1661 - 1665   2011.12( ISSN:0018-5345

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Adventitious shoots can be regenerated from the cut surface of the primary shoot and lateral branches in decapitated plants in vivo. This inherent regenerative ability of plants is useful for mass propagation. In the present study, we conducted histological observations of shoot regeneration and applied auxin and cytokinin to decapitated seedlings in four tomato cultivars. The cultivars produced different numbers of adventitious shoots after decapitation; 'Petit' produced the largest number of adventitious shoots (78.5 +/- 10.2) and 'Momotaro' produced the fewest (12.1 +/- 3.3). Histological observation of 'Petit' revealed that adventitious shoots regenerated from calli formed at the cut surface of stems. Adventitious shoot formation was inhibited by the presence of lateral branches. Shoot regeneration was prevented by application of 1-naphthaleneacetic acid to 'Petit'. Application of 6-benzyladenine promoted shoot regeneration in 'Momotaro'. These results suggest auxin synthesized de novo from the lateral branches inhibited shoot regeneration after decapitation and endogenous cytokinin might stimulate shoot regeneration. Chemical names: 1-naphthaleneacetic acid (NAA); 6-benzyladenine (BA)

    DOI: 10.21273/HORTSCI.46.12.1661

  • Chromosomal location of HWA1 and HWA2, complementary hybrid weakness genes in rice Reviewed

    Katsuyuki Ichitani, Satoru Taura, Takahiro Tezuka, Yuuya Okiyama, Tsutomu Kuboyama

    Rice   4 ( 2 )   29 - 38   2011.06( ISSN:1939-8425

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Hybrid weakness phenomena in rice reportedly have two causes: those of HWC1 and HWC2 genes and those of HWA1 and HWA2 genes. No detailed study of the latter has been reported. For this study, we first produced crosses among cultivars carrying the weakness-causing allele on the HWA1 and HWA2 loci to confirm the phenotype of the hybrid weakness and the genotypes of the cultivars on the two loci, as reported earlier. We then confirmed that these cultivars belong to Indica. Subsequent linkage analysis of HWA1 and HWA2 genes conducted using DNA markers revealed that both genes are located in the 1,637-kb region, surrounded by the same DNA markers on the long arm of chromosome 11. The possibility of allelic interaction inducing hybrid weakness is discussed.

    DOI: 10.1007/s12284-011-9062-2

  • Development of new DNA markers linked to the Fusarium wilt resistance locus Fom-1 in melon Reviewed

    Takahiro Tezuka, Keisuke Waki, Maki Kuzuya, Tomoko Ishikawa, Yasumasa Takatsu, Makoto Miyagi

    Plant Breeding   130 ( 2 )   261 - 267   2011.04( ISSN:0179-9541

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    P&gt;Use of resistance genes would be the most effective way to address Fusarium wilt caused by Fusarium oxysporum f.sp. melonis in melon. In this study, based on the BAC 31O16 sequence which contains the cluster of melon resistance gene homologue, we developed four new DNA markers linked to Fom-1, a dominant resistance gene against F. oxysporum f.sp. melonis races 0 and 2. Linkage analysis using 125 F-2 plants derived from the cross between P11 (fom-1fom-1) and MR-1 (Fom-1Fom-1) indicated that Fom-1 was located between CAPS markers C-MRGH12 and 62-CAPS at a distance of 0.4 and 1.2 cM, respectively. Map position of Fom-1 was confirmed using another mapping population consisting of 104 recombinant inbred lines derived from the cross P11 x MR-1. The analysis of 43 fixed lines and 27 commercial F-1 hybrids showed that the developed DNA markers linked to Fom-1 correctly predicted the genotype in relation to the variety, suggesting that the region around the Fom-1 locus is very polymorphic or there is no linkage disequilibrium between the locus and the marker loci.

    DOI: 10.1111/j.1439-0523.2010.01800.x

  • Hybrid lethality observed in interspecific hybrids between wild tobacco species in section Suaveolentes and cultivated tobacco

    Takahiro Tezuka, Wataru Marubashi

    Journal of Crop Research   56   9 - 15   2011( ISSN:1882-885X

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    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)  

    Inviability of hybrids, often referred to as hybrid lethality, is a type of reproductive isolation. Hybrid lethality is observed in some plant species, including <i>Nicotiana</i> species, and can be an obstacle when desirable genes are to be introduced into cultivated species by wide hybridization. Wild species in <i>Nicotiana</i> section <i>Suaveolentes</i> (which consists of species restricted to Australasia and Africa) yield inviable hybrids after crosses with the cultivated species <i>N. tabacum</i>. Genetic studies have revealed that hybrid lethality in the cross <i>N. tabacum</i>×<i>N. debneyi</i> is caused by the interaction between gene(s) on the Q chromosome of <i>N. tabacum</i> and a single dominant gene in <i>N. debneyi</i>. Furthermore, programmed cell death with apoptotic features is involved in hybrid lethality. Some factors related to hybrid lethality have been elucidated in the cross <i>N. suaveolens</i>×<i>N. tabacum</i>.

    DOI: 10.18964/jcr.56.0_9

    CiNii Article

  • L-2-aminooxy-3-phenylpropionic acid (AOPP) controls browning and promotes adventitious bud formation in Neofinetia falcata in vitro Reviewed

    Kazuhiko Mitsukuri, Masahumi Johkan, Satoshi Yamasaki, Hideyuki Tanaka, Takahiro Tezuka, Kei-ichiro Mishiba, Masayuki Oda

    Journal of the Japanese Society for Horticultural Science   79 ( 4 )   367 - 371   2010.10( ISSN:1882-3351

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    DOI: 10.2503/jjshs1.79.367

    CiNii Article

    J-GLOBAL

  • Seven of eight species in Nicotiana section Suaveolentes have common factors leading to hybrid lethality in crosses with Nicotiana tabacum Reviewed

    Takahiro Tezuka, Tsutomu Kuboyama, Toshiaki Matsuda, Wataru Marubashi

    Annals of Botany   106 ( 2 )   267 - 276   2010.08( ISSN:0305-7364

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    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    DOI: 10.1093/aob/mcq114

    PubMed

  • Effects of anti-browning conditioning on adventitious bud formation in Neofinetia falcata Hu. in vitro Reviewed

    Kazuhiko Mitsukuri, Masahumi Johkan, Satoshi Yamasaki, Takahiro Tezuka, Kei-ichiro Mishiba, Masayuki Oda

    Horticulture, Environment, and Biotechnology   50 ( 4 )   295 - 299   2009.08

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

  • Construction of a linkage map and identification of DNA markers linked to Fom-1, a gene conferring resistance to Fusarium oxysporum f.sp. melonis race 2 in melon Reviewed

    Takahiro Tezuka, Keisuke Waki, Kazutoshi Yashiro, Maki Kuzuya, Tomoko Ishikawa, Yasumasa Takatsu, Makoto Miyagi

    Euphytica   168 ( 2 )   177 - 188   2009.07( ISSN:00142336

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    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    DOI: 10.1007/s10681-009-9881-z

  • Characteristic evaluation for gene resources of genus Cucumis

    Yuichi Matsumoto, Yasumasa Takatsu, Kenji Miyamoto, Takahiro Tezuka, Yoshiteru Sakata, Makoto Miyagi

    Bulletin of the Plant Biotechnology Institute, Ibaraki Agricultural Center   11   23 - 32   2009.03

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    Publishing type:Research paper (bulletin of university, research institution)   International / domestic magazine:Domestic journal  

    CiNii Article

  • Possible involvement of genes on the Q chromosome of Nicotiana tabacum in expression of hybrid lethality and programmed cell death during interspecific hybridization to Nicotiana debneyi Reviewed

    Takahiro Tezuka, Tsutomu Kuboyama, Toshiaki Matsuda, Wataru Marubashi

    Planta   226 ( 3 )   753 - 764   2007.08( ISSN:0032-0935

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    DOI: 10.1007/s00425-007-0522-2

    PubMed

    CiNii Article

  • Genomic factors lead to programmed cell death during hybrid lethality in interspecific hybrids between Nicotiana tabacum and N. debneyi Reviewed

    Takahiro Tezuka, Wataru Marubashi

    SABRAO Journal of Breeding and Genetics   38 ( 2 )   69 - 81   2006.12

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    CiNii Article

  • Hybrid lethality in interspecific hybrids between Nicotiana tabacum and N. suaveolens: evidence that the Q chromosome causes hybrid lethality based on Q-chromosome-specific DNA markers Reviewed

    Takahiro Tezuka, Wataru Marubashi

    Theoretical and Applied Genetics   112 ( 6 )   1172 - 1178   2006.04( ISSN:0040-5752

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    DOI: 10.1007/s00122-006-0219-0

    PubMed

    CiNii Article

  • Nicotiana属種間雑種に現れるプログラム細胞死を伴う雑種致死に関する遺伝学的研究

    手塚孝弘

    東京農工大学大学院連合農学研究科生物生産学専攻植物生産学連合講座博士論文   2006.03

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    Authorship:Lead author, Corresponding author   Publishing type:Doctoral thesis  

    指導教員:丸橋亘;指導教員・主査:松田智明;第1副指導教員・副査:久保山勉;第2副指導教員・副査:金子幸雄;副査:松澤康男、平田豊

  • The Q chromosome of Nicotiana tabacum controls hybrid lethality in interspecific hybrids between N. tabacum and N. debneyi

    Takahiro Tezuka, Tsutomu Kuboyama, Toshiaki Matsuda, Wataru Marubashi

    B-21   2005.03

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    Authorship:Lead author   Publishing type:Research paper (international conference proceedings)  

  • Development of Q-chromosome-specific DNA markers in tobacco and their use for identification of a tobacco monosomic line Reviewed

    Takahiro Tezuka, Katsura Onosato, Saori Hijishita, Wataru Marubashi

    Plant and Cell Physiology   45 ( 12 )   1863 - 1869   2004.12( ISSN:0032-0781

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    DOI: 10.1093/pcp/pch204

    PubMed

    CiNii Article

  • Apoptotic cell death observed during the expression of hybrid lethality in interspecific hybrids between Nicotiana tabacum and N. suaveolens Reviewed

    Takahiro Tezuka, Wataru Marubashi

    Breeding Science   54 ( 1 )   59 - 66   2004.03( ISSN:1344-7610

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Hybrid seedlings from the cross <i>Nicotiana tabacum</i> ×<i>N. suaveolens</i>, obtained by test-tube pollination and ovule culture, expressed lethality at 28°C. Characteristic lethal symptoms in these hybrid seedlings consisted of browning of hypocotyls and roots. One hundred and seventeen hybrid seedlings were eventually obtained by the use of test-tube pollination and ovule culture. Hybrid seedlings maintained at 36°C did not express any lethal symptoms. Hybrid seedlings used for further experiments were transferred to 36°C immediately after germination at 28°C. When hybrid seedlings cultured at 36°C were transferred to 28°C, their growth stopped and lethal symptoms were expressed. During the progressive expression of lethality, apoptotic features such as chromatin condensation, nuclear fragmentation and DNA fragmentation were detected. On the other hand, there was no sign of apoptotic cell death in the hybrid seedlings at 36°C. Based on the observation that the same lethal symptoms and the same apoptotic features were observed in the reciprocal cross, <i>N. suaveolens</i> ×<i>N. tabacum</i>, we suggest that not only the underlying causes of hybrid lethality but also the underlying causes of apoptotic cell death are due to the interaction of coexisting heterogeneous genomes, rather than to the effect of cytoplasmic genes. Furthermore, the progression of apoptotic cell death in the cross <i>N. tabacum</i> × <i>N. suaveolens</i> began in stems and roots, followed by leaves.<br>

    DOI: 10.1270/jsbbs.54.59

    CiNii Article

  • The role of ethylene in hybrid lethality accompanied by programmed cell death in tobacco Reviewed

    Wataru Marubashi, Tetsuya Yamada, Takahiro Tezuka

    77 - 84   2003.11

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    Publishing type:Research paper (international conference proceedings)  

  • タバコ属種間雑種(Nicotiana tabacum × N. debneyiおよびN. tabacum × N. suaveolens)の致死過程において認められたアポトーシス

    手塚孝弘

    茨城大学大学院農学研究科資源生物科学専攻修士論文   2003.03

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    Authorship:Lead author, Corresponding author   Publishing type:Master’s thesis  

    指導教員・主査:丸橋亘;副査:久保山勉、久留主泰朗

  • ワサビダイコン(Armoracia rusticana Gaertn.)の根ざし繁殖株および再分化個体においてInter-SSR PCRによって検出された変異

    手塚孝弘

    茨城大学農学部資源生物科学科卒業論文   2001.03

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    Authorship:Lead author, Corresponding author   Publishing type:Thesis (other)  

    指導教員:丹羽勝

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Books and Other Publications

  • Herbaceous Plants: Cultivation Methods, Grazing and Environmental Impacts

    Takahiro Tezuka( Role: Sole author ,  Hybrid lethality in Nicotiana: a review with special attention to interspecific crosses between species in sect. Suaveolentes and N. tabacum)

    Nova Science Publishers, New York  2013.05  ( ISBN:9781626187290

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    Total pages:171   Responsible for pages:69-94   Book type:Scholarly book Participation form:Corresponding Author

    Scopus

  • Botany Reviewed

    Takahiro Tezuka( Role: Sole author ,  Hybrid lethality in the genus Nicotiana)

    InTech, Rijeka, Croatia  2012.03  ( ISBN:9789535103554

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    Total pages:238   Responsible for pages:191-210   Book type:Scholarly book Participation form:Corresponding Author

    DOI: 10.5772/33544

MISC

  • DNAマーカーを用いた緑肉ネット系メロン「ひたち交3号」の判別法 International journal

    石川友子, 宮城慎, 葛谷真輝, 手塚孝弘

    平成20年度「関東東海北陸農業」研究成果情報   2008

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    Publishing type:Internal/External technical report, pre-print, etc.   International / domestic magazine:Domestic journal  

Presentations

  • SSRマーカーを用いたトウガラシ属の系統解析および種間交雑における雑種弱勢をはじめとする生殖隔離機構の調査 Domestic conference

    世故捷之助, 横井修司, 手塚孝弘

    日本育種学会第145回講演会  2024.03  日本育種学会

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    Presentation type:Oral presentation (general)  

    Venue:東京大学  

  • Production and identification of an interspecific hybrid between Artemisia princeps and A. vulgaris Domestic conference

    Xu Tianying, Shuji Yokoi, Takahiro Tezuka

    日本育種学会第145回講演会  2024.03  日本育種学会

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    Presentation type:Oral presentation (general)  

    Venue:東京大学  

  • Bulked segregant RNA-Seqを用いたタバコ雑種致死原因遺伝HLA1の遺伝的マッピング Domestic conference

    永井翔大, 横井修司, 手塚孝弘

    日本育種学会第145回講演会  2024.03  日本育種学会

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    Presentation type:Oral presentation (general)  

    Venue:東京大学  

  • タバコ致死性種間交雑(Nicotiana amplexicaulis × N. tabacum)において高頻度で出現する生存雑種で見出された雑種致死原因遺伝子HLA1周辺領域の欠失 Domestic conference

    永井翔大, 松本果歩, 川口謙二, 横井修司, 手塚孝弘

    日本育種学会第143回講演会  2023.03  日本育種学会

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    Presentation type:Oral presentation (general)  

    Venue:静岡大学  

  • タバコ種間交雑における雑種致死原因遺伝子HLA1の起源の探索 Domestic conference

    西出七虹, 横井修司, 手塚孝弘

    日本育種学会第143回講演会  2023.03  日本育種学会

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    Presentation type:Oral presentation (general)  

    Venue:静岡大学  

  • タバコ異倍数性種間交雑に生じる種子発育不全および子房落下の遺伝学的解析 Domestic conference

    木田茉奈美, 横井修司, 手塚孝弘

    日本育種学会第143回講演会  2023.03  日本育種学会

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    Presentation type:Oral presentation (general)  

    Venue:静岡大学  

  • タバコ雑種致死原因遺伝子HLA1周辺領域のハプロタイプ解析 Domestic conference

    永井翔大, 横井修司, 手塚孝弘

    日本育種学会第141回講演会  2022.03  日本育種学会

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    Presentation type:Poster presentation  

    Venue:摂南大学(オンライン開催)  

  • トウガラシ属種間交雑で生じる雑種弱勢における温度感受性および糖蓄積 Domestic conference

    白柿薫平, 古川一, 横井修司, 手塚孝弘

    日本育種学会第138回講演会  2020.10  日本育種学会

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    Presentation type:Poster presentation  

    Venue:オンライン  

  • トウガラシ雑種弱勢における系統地理学的解析 Domestic conference

    白柿薫平, 横井修司, 手塚孝弘

    日本進化学会第22回オンライン大会  2020.09  日本進化学会

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    Presentation type:Poster presentation  

    Venue:オンライン  

  • イネコアコレクションを用いた栄養生長期の相転移因子の解析 Domestic conference

    山本真璃, 手塚孝弘, 横井修司

    日本育種学会第137回講演会  2020.03  日本育種学会

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    Presentation type:Poster presentation  

  • イネ種子のγ-オリザノール含量を規定する候補因子の探索 Domestic conference

    船越孝之, 桧原健一郎, 小川拓水, 手塚孝弘, 太田大策, 横井修司

    日本育種学会第137回講演会  2020.03  日本育種学会

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    Presentation type:Oral presentation (general)  

  • トウガラシ属種間交雑で生じる雑種弱勢の表現型解析 Domestic conference

    白柿薫平, 横井修司, 手塚孝弘

    日本育種学会第137回講演会  2020.03  日本育種学会

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    Presentation type:Oral presentation (general)  

  • トウガラシ栽培種におけるSSRマーカーを用いた系統解析および雑種弱勢原因遺伝子の分布の調査 Domestic conference

    立田智輝, 横井修司, 手塚孝弘

    日本育種学会第137回講演会  2020.03  日本育種学会

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    Presentation type:Poster presentation  

  • 葉緑体DNAを用いたNicotiana suaveolens 8系統の系統解析 Domestic conference

    定久公美, 何海, 横井修司, 手塚孝弘

    日本育種学会第137回講演会  2020.03  日本育種学会

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    Presentation type:Poster presentation  

  • イネコアコレクションを用いたγ-オリザノール含量に相関のある因子の形態学的・分子遺伝学的解析 Domestic conference

    船越孝之, 小川拓水, 手塚孝弘, 太田大策, 横井修司

    日本育種学会第136回講演会  2019.09  日本育種学会

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    Presentation type:Poster presentation  

  • タバコ種間交雑における子房落下現象には母親の過剰な高次倍数性および種子発育不全が関与する Domestic conference

    何海, 横井修司, 手塚孝弘

    日本育種学会第136回講演会  2019.09  日本育種学会

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    Presentation type:Oral presentation (general)  

  • イネコアコレクションを用いた種子中のγ­オリザノール含量を規定する因子の探索 Domestic conference

    船越孝之, 小川拓水, 手塚孝弘, 太田大策, 横井修司

    日本育種学会第135回講演会  2019.03  日本育種学会

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    Presentation type:Oral presentation (general)  

  • ソルガムにおけるjuvenile-to-adult相転換について Domestic conference

    橋本舜平, 手塚孝弘, 横井修司

    日本育種学会第135回講演会  2019.03  日本育種学会

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    Presentation type:Oral presentation (general)  

  • ソルガムにおける夜低温誘導型開花にはSbPRR37が関与する Domestic conference

    橋本舜平, 手塚孝弘, 横井修司

    第60回日本植物生理学会年会  2019.03  日本植物生理学会

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    Presentation type:Oral presentation (general)  

  • Distinct mechanisms are involved in seed abortion and ovary abscission in interploidy-interspecific crosses between Nicotiana suaveolens and N. tabacum International conference

    Hai He, Shuji Yokoi, Takahiro Tezuka

    6th Plant Dormancy Symposium 2018  2018.10 

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    Presentation type:Poster presentation  

  • 植物の生殖隔離機構解明に向けて ―多様性と統一性― Domestic conference

    手塚孝弘, 一谷勝之, 久保山勉

    日本育種学会2018年秋季大会ワークショップ  2018.09  日本育種学会

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    Presentation type:Symposium, workshop panel (public)  

  • イネ雑種弱勢の遺伝と病徴 Domestic conference

    一谷勝之, 久保山勉, 手塚孝弘

    日本育種学会第134回講演会,W05 植物の生殖隔離機構解明に向けて ―多様性と統一性―  2018.09  日本育種学会

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    Presentation type:Symposium, workshop panel (public)  

  • タバコの異倍数性種間交雑に認められる子房落下および種子発育不全 Domestic conference

    手塚孝弘, 何海

    日本育種学会第134回講演会,W05 植物の生殖隔離機構解明に向けて ―多様性と統一性―  2018.09  日本育種学会

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    Presentation type:Symposium, workshop panel (public)  

  • タバコの異倍数性種間交雑後の子房落下現象における脱離関連遺伝子の発現解析 Domestic conference

    何海, 横井修司, 手塚孝弘

    日本育種学会第134回講演会  2018.09  日本育種学会

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  • 形態形質とrDNAのITS領域を用いたトウガラシ属栽培種5種の系統解析 Domestic conference

    白柿薫平, 横井修司, 手塚孝弘

    日本育種学会第134回講演会  2018.09  日本育種学会

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  • イネ種子のγ-oryzanol含量を規定する因子の解明と形態マーカーの開発 Domestic conference

    船越孝之, 小川拓水, 手塚孝弘, 太田大策, 横井修司

    近畿作物・育種研究会第185回例会  2018.06  近畿作物・育種研究会

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    Presentation type:Oral presentation (general)  

  • Hybrid dwarfism observed in the cross between Capsicum annuum and C. chinense International conference

    Kumpei Shiragaki, Shuji Yokoi, Takahiro Tezuka

    The 25th International Congress on Sexual Plant Reproduction  2018.06  International Association of Sexual Plant Reproduction Research

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    Presentation type:Poster presentation  

  • Comparative analysis of different types of reproductive isolation observed in interspecific-interploidy crosses between two Nicotiana suaveolens accessions and N. tabacum International conference

    Hai He, Shuji Yokoi, Takahiro Tezuka

    The 25th International Congress on Sexual Plant Reproduction  2018.06  International Association of Sexual Plant Reproduction Research

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    Presentation type:Poster presentation  

  • Genetic mapping of the HLA1 locus causing hybrid lethality in tobacco interspecific hybrids International conference

    Takahiro Tezuka, Sae Imagawa, Akira Hasegawa, Hai He, Shuji Yokoi

    The 25th International Congress on Sexual Plant Reproduction  2018.06  International Association of Sexual Plant Reproduction Research

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    Presentation type:Poster presentation  

  • ヒエ[Echinochloa esculenta (A. Braun) H. Scholz]半矮性変異系統の表現型解析 Domestic conference

    濱本亮, 手塚孝弘, 横井修司

    日本育種学会第133回講演会  2018.03  日本育種学会

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    Presentation type:Poster presentation  

  • イネにおける塩ストレスと光周性開花経路とのクロストークに関わる因子の解析 Domestic conference

    橋本舜平, 手塚孝弘, 横井修司

    日本育種学会第133回講演会  2018.03  日本育種学会

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    Presentation type:Oral presentation (general)  

  • タバコ野生種Nicotiana suaveolens 2系統と栽培種N. tabacumとの異倍数性種間交雑に生じる異なる生殖隔離の比較解析 Domestic conference

    何海, 横井修司, 手塚孝弘

    日本育種学会第133回講演会  2018.03  日本育種学会

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    Presentation type:Oral presentation (general)  

  • Nicotiana tabacumとの種間雑種において異なる致死性を示すN. occidentalis 6系統の系統解析 Domestic conference

    川口謙二, 大矢祐一郎, 森川利信, 横井修司, 手塚孝弘

    日本育種学会第132回講演会  2017.10  日本育種学会

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    Presentation type:Oral presentation (general)  

  • イネにおけるHWA1とHWA2およびHWC1とHWC2による雑種弱勢の比較解析 Domestic conference

    白柿薫平, 佐原敏基, 一谷勝之, 久保山勉, 松村篤, 森川利信, 手塚孝弘

    日本育種学会第132回講演会  2017.10  日本育種学会

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  • タバコの異倍数性種間交雑(Nicotiana suaveolens PI 555565 × N. tabacum)における子房落下現象の組織学的解析 Domestic conference

    何海, 横井修司, 手塚孝弘

    日本育種学会第132回講演会  2017.10  日本育種学会

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  • 高温条件がHWA1とHWA2を原因とするイネ雑種弱勢の表現型と遺伝子発現に及ぼす影響 Domestic conference

    小幡正彬, 白柿薫平, 一谷勝之, 久保山勉, 松村篤, 森川利信, 横井修司, 手塚孝弘

    日本育種学会第132回講演会  2017.10  日本育種学会

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  • ITS領域およびSSRマーカーによる沖縄のコーヒーノキの系統解析 Domestic conference

    川口謙二, 森田裕之, 手塚孝弘

    園芸学会平成29年度秋季大会  2017.09  園芸学会

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    Presentation type:Oral presentation (general)  

  • AFLPおよびSSRマーカーによるタバコ野生種Nicotiana suaveolens 8系統の系統解析 Domestic conference

    何海, 森川利信, 横井修司, 手塚孝弘

    日本育種学会第130回講演会  2016.09  日本育種学会

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  • タバコ野生種Nicotiana suaveolensの倍数性が栽培種N. tabacumとの交雑に認められる生殖隔離に及ぼす影響 Domestic conference

    何海, 飯塚貴大, 前川真穂, 森川利信, 簗瀬雅則, 横井修司, 小田雅行, 手塚孝弘

    日本育種学会第129回講演会  2016.03  日本育種学会

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  • Nicotiana tabacumとN. occidentalis 5系統との種間雑種で認められる雑種致死に関与するゲノムの推定 Domestic conference

    大矢祐一郎, 前川真穂, 飯塚貴大, 森川利信, 簗瀬雅則, 横井修司, 小田雅行, 手塚孝弘

    日本育種学会第129回講演会  2016.03  日本育種学会

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  • タバコ種間雑種(Nicotiana suaveolens × N. tabacum)に生じる雑種致死に及ぼすフェニルアラニンアンモニアリアーゼ(PAL)阻害剤の影響 Domestic conference

    中村理恵, 山田哲也, 丸橋亘, 小田雅行, 手塚孝弘

    日本育種学会第129回講演会  2016.03  日本育種学会

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  • タバコ種間交雑(Nicotiana amplexicaulis × N. tabacum)に認められた生存雑種高頻度出現現象 Domestic conference

    板倉広樹, 中村理恵, 森川利信, 簗瀬雅則, 横井修司, 手塚孝弘

    日本育種学会第128回講演会  2015.09  日本育種学会

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  • 生物研世界イネコアコレクションにおける雑種弱勢遺伝子Hwa1-1,Hwa2-1の分布 Domestic conference

    一谷勝之, 古賀功明, 坂口瑛進, 田浦悟, 石川隆二, 手塚孝弘, 久保山勉

    日本育種学会第127回講演会  2015.03  日本育種学会

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  • HWA1とHWA2によって生じるイネ雑種弱勢の遺伝子発現解析 Domestic conference

    白柿薫平, 一谷勝之, 久保山勉, 簗瀬雅則, 森川利信, 手塚孝弘

    日本育種学会第127回講演会  2015.03  日本育種学会

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  • Nicotiana debneyiがもつ雑種致死原因遺伝子HLA1の連鎖解析 Domestic conference

    喜多村直人, 森川利信, 簗瀬雅則, 尾形善之, 小田雅行, 手塚孝弘

    日本育種学会第127回講演会  2015.03  日本育種学会

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  • SSR,RAPDおよびSCARから見た日本在来イネ品種の遺伝的多様性 Domestic conference

    石田真依, 簗瀬雅則, 手塚孝弘, 森川利信

    日本育種学会第127回講演会  2015.03  日本育種学会

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  • Genetics of F1 hybrid lethality in tobacco Invited International conference

    Takahiro Tezuka

    Japanese Solanaceae Genomics Initiative (JSOL) 10th International Symposium on Solanaceae Genomics  2013.11  Japanese Solanaceae Genomics Initiative (JSOL)

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    Presentation type:Oral presentation (invited, special)  

  • Nicotiana benthamianaとN. excelsiorの種間雑種で認められる不稔性の評価 Domestic conference

    喜多村直人, 森川利信, 簗瀬雅則, 小田雅行, 手塚孝弘

    日本育種学会第124回講演会  2013.10  日本育種学会

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  • エンバクのサツマイモネコブセンチュウ抵抗性の遺伝分析とDNAマーカーの探索 Domestic conference

    中岡史裕, 桂真昭, 立石靖, 簗瀬雅則, 手塚孝弘, 森川利信

    日本育種学会第124回講演会  2013.10  日本育種学会

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  • 硼素中性子捕捉反応(BNCR)によるオオムギとタバコの突然変異誘発 Domestic conference

    岩本千里, 簗瀬雅則, 手塚孝弘, 森川利信, 服部能英, 切畑光統

    日本育種学会第124回講演会  2013.10  日本育種学会

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  • イネ雑種弱勢遺伝子HWA1,HWA2の高密度連鎖解析 Domestic conference

    一谷勝之, 浦田千恵子, 田浦悟, 手塚孝弘, 沖山友哉, 久保山勉, 佐藤宗治

    日本育種学会第123回講演会  2013.03  日本育種学会

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  • トマト全茎切断苗における形態異常の発生 Domestic conference

    小野正貴, 田中秀幸, 手塚孝弘, 古川一, 小田雅行

    園芸学会平成25年度春季大会  2013.03  園芸学会

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  • ナスおよびピーマンのin vitro全茎切断におけるAOPP処理が不定芽形成に及ぼす影響 Domestic conference

    廣瀬亮平, 藤岡ももこ, 田中秀幸, 手塚孝弘, 小田雅行

    園芸学会平成25年度春季大会  2013.03  園芸学会

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  • ナスのin vitro全茎切断における窒素欠乏前処理がシュート形成に及ぼす影響 Domestic conference

    田中秀幸, 藤岡ももこ, 黒田恵子, 古川一, 手塚孝弘, 小田雅行

    園芸学会平成25年度春季大会  2013.03  園芸学会

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  • 硼素中性子捕捉反応(BNCR)の植物突然変異育種への応用 Domestic conference

    岩本千里, 手塚孝弘, 簗瀬雅則, 森川利信

    近畿作物・育種研究会第174回例会  2012.12  近畿作物・育種研究会

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  • トマト全茎切断苗の特性および着果の季節変動 Domestic conference

    小野正貴, 田中秀幸, 手塚孝弘, 古川一, 小田雅行

    平成24年度園芸学会近畿支部大阪大会  2012.09  園芸学会近畿支部

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  • 概日時計が遺伝子組換えレタスの有用物質生産に及ぼす影響 Domestic conference

    岡村憲一, 福田弘和, 手塚孝弘

    日本生物環境工学会2012年大会  2012.09  日本生物環境工学会

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    Presentation type:Oral presentation (general)  

  • Complete decapitation and rootstock culture in vitro are efficient methods for shoot regeneration from eggplant hypocotyls International conference

    Hideyuki Tanaka, Yuta Yokoyama, Satoshi Yamasaki, Masataka Ono, Takahiro Tezuka, Masayuki Oda

    American Society for Horticultural Science 109th Annual Conference  2012.08  American Society for Horticultural Science

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    Presentation type:Poster presentation  

  • Adventitious bud formation promoted by L-2-aminooxy-3-phenylpropionic acid in tomato plants treated with the complete decapitation method in vitro International conference

    Hideyuki Tanaka, Kazuhiko Mitsukuri, Satoshi Yamasaki, Ryohei Hirose, Takahiro Tezuka, Masayuki Oda

    American Society for Horticultural Science 109th Annual Conference  2012.08  American Society for Horticultural Science

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    Presentation type:Poster presentation  

  • HWA1とHWA2を原因遺伝子とするイネ雑種弱勢における細胞死 Domestic conference

    飯塚貴大, 一谷勝之, 久保山勉, 小田雅行, 手塚孝弘

    日本育種学会第121回講演会  2012.03  日本育種学会

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  • The Q chromosome is responsible for hybrid lethality in crosses between Nicotiana tabacum and Nicotiana simulans International conference

    Takahiro Tezuka, Chihiro Matsuo, Toshiaki Okamori, Takahiro Iizuka, Masayuki Oda, Wataru Marubashi

    SOL & ICuGI 2011, 8th Solanaceae and 2nd Cucurbitaceae Genome Joint Conference  2011.11 

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  • Evaluation of RAPD variation and ploidy changes in genetic tumor of hybrids between Solanum lycopersicum and S. habrochaites International conference

    Hidenobu Okinaka, Masayuki Oda, Takahiro Tezuka

    SOL & ICuGI 2011, 8th Solanaceae and 2nd Cucurbitaceae Genome Joint Conference  2011.11 

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  • A simple and efficient method for shoot regeneration from hypocotyl in eggplant in vitro International conference

    Hideyuki Tanaka, Yuta Yokoyama, Satoshi Yamasaki, Takahiro Tezuka, Masayuki Oda

    SOL & ICuGI 2011, 8th Solanaceae and 2nd Cucurbitaceae Genome Joint Conference  2011.11 

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    Presentation type:Poster presentation  

  • トマトのin vitro全茎切断におけるPAL阻害剤AOPPによる不定芽形成の促進 Domestic conference

    箕作和彦, 田中秀幸, 山崎識知, 手塚孝弘, 小田雅行

    園芸学会平成23年度秋季大会  2011.09  園芸学会

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    Presentation type:Oral presentation (general)  

  • トマト種間雑種Solanum lycopersicum × S. habrochaitesにおける遺伝的腫瘍の発現 Domestic conference

    冲中秀伸, 小田雅行, 手塚孝弘

    第29回日本植物細胞分子生物学会(福岡)大会  2011.09  日本植物細胞分子生物学会

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    Presentation type:Oral presentation (general)  

  • ナスの胚軸培養におけるPAL阻害剤AOPPによる不定芽形成の促進 Domestic conference

    田中秀幸, 上田晴香, 箕作和彦, 手塚孝弘, 小田雅行

    園芸学会平成23年度秋季大会  2011.09  園芸学会

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  • Nicotiana debneyiはN. tabacumとの交雑における雑種致死の原因となる単一の優性遺伝子をもつ Domestic conference

    飯塚貴大, 久保山勉, 丸橋亘, 小田雅行, 手塚孝弘

    日本育種学会第119回講演会  2011.03  日本育種学会

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    Presentation type:Oral presentation (general)  

  • Q染色体特異的SSRマーカーを用いたタバコ種間雑種(Nicotiana tabacum × N. ingulba)における雑種致死原因染色体の特定 Domestic conference

    松尾千廣, 飯塚貴大, 丸橋亘, 小田雅行, 手塚孝弘

    日本育種学会第119回講演会  2011.03  日本育種学会

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    Presentation type:Oral presentation (general)  

  • イネ雑種弱勢遺伝子HWA1,HWA2の連鎖分析 Domestic conference

    一谷勝之, 田浦悟, 手塚孝弘, 沖山友哉, 久保山勉

    日本育種学会第119回講演会  2011.03  日本育種学会

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    Presentation type:Oral presentation (general)  

  • タバコ種間雑種における雑種致死の原因となるQ染色体に相当する連鎖群の同定 Domestic conference

    松尾千廣, 丸橋亘, 小田雅行, 手塚孝弘

    日本育種学会第119回講演会  2011.03  日本育種学会

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    Presentation type:Oral presentation (general)  

  • ユーカリ・グロブラス挿し穂の調製および化学的処理による発根促進 Domestic conference

    井本洋輔, 手塚孝弘, 箕作和彦, 山崎識知, 田中秀幸, 小田雅行

    園芸学会平成23年度春季大会  2011.03  園芸学会

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    Presentation type:Oral presentation (general)  

  • 球根ベゴニアの逆挿しにおける植物成長調節物質による不定芽形成の促進 Domestic conference

    田中秀幸, 塩崎修志, 山崎識知, 箕作和彦, 手塚孝弘, 小田雅行

    園芸学会平成23年度春季大会  2011.03  園芸学会

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    Presentation type:Poster presentation  

  • Nicotiana tabacumとN. debneyiの種間交雑において認められる雑種致死の遺伝解析 Domestic conference

    飯塚貴大, 久保山勉, 丸橋亘, 小田雅行, 手塚孝弘

    近畿作物;育種研究会第170回例会  2010.12  近畿作物・育種研究会

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    Presentation type:Oral presentation (general)  

  • タバコ栽培種とSuaveolentes節に属するタバコ野生種との種間雑種に認められる雑種致死 Invited Domestic conference

    手塚孝弘, 丸橋亘

    近畿作物・育種研究会第170回例会  2010.12  近畿作物・育種研究会

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    Presentation type:Oral presentation (invited, special)  

  • トマトの台木培養による不定芽形成の促進 Domestic conference

    箕作和彦, 廣瀬亮平, 山崎識知, 田中秀幸, 手塚孝弘, 小田雅行

    園芸学会平成22年度秋季大会  2010.09  園芸学会

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    Presentation type:Oral presentation (general)  

  • Nicotiana tabacumとSuaveolentes節に属する21種50系統の野生種との交雑における雑種致死の発現 Domestic conference

    飯塚貴大, 小田雅行, 手塚孝弘

    日本育種学会第117回講演会  2010.03  日本育種学会

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    Presentation type:Poster presentation  

  • 球根ベゴニアの小葉片挿しにおける内生オーキシン濃度と不定芽形成との関係 Domestic conference

    田中秀幸, 塩崎修志, 淨閑正史, 箕作和彦, 山崎識知, 手塚孝弘, 小田雅行

    平成21年度園芸学会近畿支部和歌山大会  2009.08  園芸学会近畿支部

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    Presentation type:Oral presentation (general)  

  • Nicotiana tabacumとN. occidentalisの種間雑種で認められる雑種致死に関与するゲノムの推定 Domestic conference

    手塚孝弘, 小田雅行, 丸橋亘

    日本育種学会第115回講演会  2009.03  日本育種学会

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    Presentation type:Poster presentation  

  • Effects of anti-browning conditioning on adventitious bud formation in Neofinetia falcata H. H. Hu in vitro International conference

    Kazuhiko Mitsukuri, Masahumi Johkan, Satoshi Yamasaki, Takahiro Tezuka, Kei-ichiro Mishiba, Masayuki Oda

    The first Asian Horticultural Congress (AHC) 2008  2008.12 

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    Presentation type:Poster presentation  

  • トマト肥料制限苗化の主要因 Domestic conference

    中西孝夫, 手塚孝弘, 小田雅行

    平成20年度園芸学会近畿支部京都大会  2008.09  園芸学会近畿支部

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    Presentation type:Poster presentation  

  • フウランの幼植物上部培養におけるAOPPによる不定芽形成の促進 Domestic conference

    箕作和彦, 淨閑正史, 森源治郎, 手塚孝弘, 小田雅行

    園芸学会平成20年度秋季大会  2008.09  園芸学会

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    Presentation type:Oral presentation (general)  

  • トマトにおける肥料制限苗の生育と収量 Domestic conference

    中西孝夫, 手塚孝弘, 大森弘美, 小田雅行

    園芸学会平成20年度秋季大会  2008.09  園芸学会

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    Presentation type:Poster presentation  

  • メロンつる割病抵抗性遺伝子Fom-1に連鎖するDNAマーカーの有効性の評価 Domestic conference

    手塚孝弘, 和気慶介, 葛谷真輝, 石川友子, 高津康正, 宮城慎

    日本育種学会第113回講演会  2008.03  日本育種学会

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    Presentation type:Poster presentation  

  • メロンつる割病菌レース2に対する抵抗性遺伝子のマッピング Domestic conference

    手塚孝弘, 和気慶介, 八城和敏, 葛谷真輝, 石川友子, 高津康正, 宮城慎

    日本育種学会第112回講演会  2007.09  日本育種学会

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    Presentation type:Oral presentation (general)  

  • SSRマーカーを用いたメロンF1系統へのトレーサビリティ付与 Domestic conference

    石川友子, 手塚孝弘, 葛谷真輝, 大澤良, 高津康正

    園芸学会平成19年度春季大会  2007.03  園芸学会

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    Presentation type:Oral presentation (general)  

  • Nicotiana tabacumとSuaveolentes節に属する9種の野生種との種間交雑における雑種致死の発現とその原因となる染色体 Domestic conference

    手塚孝弘, 久保山勉, 松田智明, 丸橋亘

    日本育種学会第110回講演会  2006.09  日本育種学会

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    Presentation type:Oral presentation (general)  

  • Nicotiana tabacumのQ染色体はタバコ属種間雑種(N. tabacum × N. debneyi)に認められる雑種致死に伴うプログラム細胞死を引き起こす Domestic conference

    手塚孝弘, 久保山勉, 松田智明, 丸橋亘

    日本育種学会第109回講演会  2006.03  日本育種学会

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    Presentation type:Oral presentation (general)  

  • The Q chromosome of Nicotiana tabacum controls hybrid lethality in interspecific hybrids between N. tabacum and N. debneyi International conference

    Takahiro Tezuka, Tsutomu Kuboyama, Toshiaki Matsuda, Wataru Marubashi

    10th International Congress of SABRAO  2005.08 

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    Presentation type:Poster presentation  

  • タバコ属種間雑種Nicotiana goodspeedii × N. tabacumでみられる致死において検出されたプログラム細胞死 Domestic conference

    佐藤義典, 手塚孝弘, 久留主泰郎, 丸橋亘

    日本育種学会第107・108回講演会  2005.08  日本育種学会

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    Presentation type:Poster presentation  

  • Q染色体特異的DNAマーカーは,Q染色体がタバコ属種間雑種(Nicotiana tabacum × N. suaveolens)に認められる致死性を制御していることを証明した Domestic conference

    手塚孝弘, 丸橋亘

    日本育種学会第106回講演会  2004.09  日本育種学会

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  • The role of ethylene in hybrid lethality accompanied by programmed cell death in tobacco Invited International conference

    Wataru Marubashi, Tetsuya Yamada, Takahiro Tezuka

    International Colloquium on Plant Biotechnology: Further Progress in Productive and Sustainable Agriculture  2003.11 

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    Presentation type:Oral presentation (invited, special)  

  • Identification of a tobacco monosomic line using Q chromosome-specific DNA markers International conference

    Takahiro Tezuka, Katsura Onosato, Saori Hijishita, Wataru Marubashi

    International Colloquium on Plant Biotechnology: Further Progress in Productive and Sustainable Agriculture  2003.11 

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    Presentation type:Poster presentation  

  • Identification of genes specifically expressed during hybrid lethality in the cells of interspecific hybrids (N. suaveolens × N. tabacum) by cDNA subtraction method International conference

    Yu Masuda, Tetsuya Yamada, Takahiro Tezuka, Tsutomu Kuboyama, Wataru Marubashi

    International Colloquium on Plant Biotechnology: Further Progress in Productive and Sustainable Agriculture  2003.11 

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    Presentation type:Poster presentation  

  • Q染色体特異的DNAマーカーを用いたタバコモノソミック系統の識別 Domestic conference

    手塚孝弘, 小野里桂, 肱下さおり, 丸橋亘

    日本育種学会第104回講演会  2003.09  日本育種学会

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    Presentation type:Oral presentation (general)  

  • cDNAサブトラクション法を用いたNicotiana suaveolens × N. tabacumの細胞における雑種致死特異的遺伝子群の同定 Domestic conference

    増田優, 山田哲也, 手塚孝弘, 久保山勉, 丸橋亘

    日本育種学会第104回講演会  2003.09  日本育種学会

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    Presentation type:Oral presentation (general)  

  • タバコ属種間雑種(Nicotiana tabacum × N. suaveolens)に認められたアポトーシス Domestic conference

    手塚孝弘, 丸橋亘

    日本育種学会第103回講演会  2003.04  日本育種学会

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    Presentation type:Oral presentation (general)  

  • タバコ属種間雑種(Nicotiana tabacum × N. debneyi)に認められたアポトーシス Domestic conference

    手塚孝弘, 丸橋亘

    日本育種学会第102回講演会  2002.08  日本育種学会

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    Presentation type:Oral presentation (general)  

  • Hot spots of somaclonal variation as detected by inter-SSR PCR in regenerants of horseradish Domestic conference

    SHIMATANI Z, TEZUKA T, ANZAI H, MARUBASHI W, NIWA M

    2000.09 

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    Presentation type:Oral presentation (general)  

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Outline of collaborative research (seeds)

  • 植物の遠縁交雑育種

  • 植物の生殖隔離に関する研究

  • 植物の分子マーカー利用育種に関する研究

  • フローサイトメトリーによる植物倍数性の解析

Grant-in-Aid for Scientific Research

  • 植物の種間・倍数性間交雑において種子発育不全が誘発する子房落下現象の分子機構解明

    Grant-in-Aid for Scientific Research(C)  2023

  • 植物の種間・倍数性間交雑において種子発育不全が誘発する子房落下現象の分子機構解明

    Grant-in-Aid for Scientific Research(C)  2022

  • 植物の種間・倍数性間交雑において種子発育不全が誘発する子房落下現象の分子機構解明

    Grant-in-Aid for Scientific Research(C)  2021

  • 植物の種間・倍数性間交雑において種子発育不全が誘発する子房落下現象の分子機構解明

    Grant-in-Aid for Scientific Research(C)  2020

  • トウガラシ属の種間雑種植物に認められる受精後交雑障壁の解明

    Grant-in-Aid for Young Scientists(B)  2020

  • トウガラシ属の種間雑種植物に認められる受精後交雑障壁の解明

    Grant-in-Aid for Young Scientists(B)  2019

  • トウガラシ属の種間雑種植物に認められる受精後交雑障壁の解明

    Grant-in-Aid for Young Scientists(B)  2018

  • トウガラシ属の種間雑種植物に認められる受精後交雑障壁の解明

    Grant-in-Aid for Young Scientists(B)  2017

  • イネにおける全3雑種弱勢現象の多様性と普遍性の遺伝育種学

    2015

  • タバコの致死性種間交雑における原因遺伝子および正常雑種高頻度出現現象の解明

    2015

  • イネにおける全3雑種弱勢現象の多様性と普遍性の遺伝育種学

    Grant-in-Aid for Scientific Research(C)  2014

  • タバコの致死性種間交雑における原因遺伝子および正常雑種高頻度出現現象の解明

    Grant-in-Aid for Young Scientists(B)  2014

  • イネにおける全3雑種弱勢現象の多様性と普遍性の遺伝育種学

    Grant-in-Aid for Scientific Research(C)  2013

  • タバコの致死性種間交雑における原因遺伝子および正常雑種高頻度出現現象の解明

    Grant-in-Aid for Young Scientists(B)  2013

  • イネにおける全3雑種弱勢現象の多様性と普遍性の遺伝育種学

    Grant-in-Aid for Scientific Research(C)  2012

  • タバコの種間交雑で認められる雑種致死の遺伝学的研究

    若手研究(スタートアップ)  2009

  • タバコの種間交雑で認められる雑種致死の遺伝学的研究

    若手研究(スタートアップ)  2008

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Other subsidies, etc.

  • タバコにおける雑種致死原因遺伝子の連鎖解析

    大阪府立大学  大阪府立大学若手研究者シーズ育成事業  2012

Charge of on-campus class subject

  • 応用生命科学特別研究実験CI

    2023   Intensive lecture   Graduate school

  • 応用生命科学特別研究実験CII

    2023   Intensive lecture   Graduate school

  • フィールド実習A

    2023   Weekly class   Undergraduate

  • 遺伝学

    2023   Weekly class   Undergraduate

  • 初年次ゼミナール

    2023   Weekly class   Undergraduate

  • 応用生物科学特別研究実験2A

    2023   Intensive lecture   Graduate school

  • 応用生物科学特別研究実験1A

    2023   Intensive lecture   Graduate school

  • 応用生物科学研究実験2B

    2023   Intensive lecture   Graduate school

  • 応用生物科学研究実験1B

    2023   Intensive lecture   Graduate school

  • 応用生物科学ゼミナール2B

    2023   Intensive lecture   Graduate school

  • 応用生物科学ゼミナール1B

    2023   Intensive lecture   Graduate school

  • 応用生物科学ゼミナール2A

    2023   Intensive lecture   Graduate school

  • 応用生物科学ゼミナール1A

    2023   Intensive lecture   Graduate school

  • 応用生物科学特別研究実験3B

    2023   Intensive lecture   Graduate school

  • 応用生物科学特別研究実験2B

    2023   Intensive lecture   Graduate school

  • 応用生物科学特別研究実験1B

    2023   Intensive lecture   Graduate school

  • 応用生物科学研究実験2A

    2023   Intensive lecture   Graduate school

  • 応用生物科学研究実験1A

    2023   Intensive lecture   Graduate school

  • 植物バイオサイエンス卒業研究

    2023   Intensive lecture   Undergraduate

  • 植物繁殖学

    2023   Weekly class   Undergraduate

  • 植物バイオサイエンス演習

    2023   Intensive lecture   Undergraduate

  • 育種・病理学基礎実験

    2023   Weekly class   Undergraduate

  • 植物科学基礎実験

    2023   Weekly class   Undergraduate

  • 植物科学英語

    2023   Intensive lecture   Undergraduate

  • 応用生物科学特別研究実験3A

    2023   Intensive lecture   Graduate school

  • フィールド実習B

    2023   Weekly class   Undergraduate

  • 遺伝育種学特論

    2023   Weekly class   Graduate school

  • 応用生物科学研究プレゼンテーション

    2023   Intensive lecture   Graduate school

  • 応用生物科学基礎実験B

    2023   Weekly class   Undergraduate

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Social Activities

  • 植物の育種と生殖隔離

    Role(s): Lecturer

    大阪府立茨木高等学校  学問発見講座  2022.07

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    Audience: High school students, Teachers

    Type:Visiting lecture

    Number of participants:18(人)

Academic Activities

  • ワークショップ企画・責任者(植物の生殖隔離機構解明に向けて ―多様性と統一性―)

    Role(s): Planning, management, etc.

    手塚孝弘, 一谷勝之, 久保山勉  2018.09

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    Type:Competition, symposium, etc. 

    日本育種学会2018年秋季大会ワークショップ