Researcher Profiles - ENDO Tatsuro

写真a

ENDO Tatsuro

Organization

Graduate School of Engineering Division of Science and Engineering for Materials, Chemistry and Biology Associate Professor
School of Engineering Department of Applied Chemistry

Affiliation

Institute of Engineering

Affiliation campus

Nakamozu Campus

Position

Graduate School of Engineering Division of Science and Engineering for Materials, Chemistry and Biology

Associate Professor 2022.04 - Now
School of Engineering Department of Applied Chemistry

Associate Professor 2022.04 - Now

Degree

修士（材料科学）（ Japan Advanced Institute of Science and Technology ）
博士（材料科学）（ Japan Advanced Institute of Science and Technology ）

Research Areas

Life Science / Biomaterials
Nanotechnology/Materials / Bio chemistry
Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering
Nanotechnology/Materials / Nano/micro-systems
Life Science / Medical systems
Life Science / Biomaterials
Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Measurement engineering
Life Science / Biomedical engineering
Nanotechnology/Materials / Analytical chemistry
Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering
Nanotechnology/Materials / Nanomaterials
Nanotechnology/Materials / Nano/micro-systems
Nanotechnology/Materials / Nanobioscience

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Research Interests

Nanotechnology
プラズモニクス
Biochip
バイオセンサー
Bioelectronics
ナノフォトニクス
ナノバイオテクノロジー
Sensor
Nanobiotechnology
Biosensing device
BioMEMS
Nanotechnology

Research subject summary

半導体微細加工技術を駆使したバイオセンシングデバイスの開発
ナノバイオデバイスの開発と生体計測への応用
ナノ構造を利用したバイオ/化学センサの開発
機能性材料を用いたナノ光学デバイスの設計・作製
ナノテクノロジーを基盤技術としたデバイス設計・開発および応用
ナノ/メソスケール光機能構造設計およびセンサ応用

Research Career

ナノ光学素子を用いたセンシングデバイスの開発

Individual

2006.04 - Now
機能性材料を用いたバイオ/化学センサーの開発

Individual

2006.04 - Now
バイオMEMS/NEMS技術を用いた生体計測デバイスの開発

Individual

2006.04 - Now
ナノテクノロジーを基盤技術とした光学素子設計・作製

Individual

2006.04 - Now

Professional Memberships

分析化学会

2011.04 - Now Domestic
電気学会

2010.03 - Now Domestic
精密工学会

2008.04 - Now Domestic
応用物理学会

2006.04 - Now Domestic
電気化学会

2002 - Now Domestic
化学とマイクロ・ナノシステム学会

2001 - Now Domestic
日本化学会

1999 - Now Domestic
Japanese Society of Enzyme Engineering
THE INSTITUTE OF ELECTRICAL ENGINEERS OF JAPAN
電気化学会
THE JAPAN SOCIETY FOR PRECISION ENGINEERING
日本化学会
THE JAPAN SOCIETY FOR ANALYTICAL CHEMISTRY
応用物理学会
THE SOCIETY FOR CHEMISTRY AND MICRO-NANO SYSTEMS
Society for Biotechnology, Japan
Japan Society of Applied Physics
Electrochemical Society of Japan
Chemical Society of Japan

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Committee Memberships (off-campus)

電気学会論文誌　センサ・マイクロマシン部門誌　論文編集委員・幹事電気学会

2009.04 - Now
会長日本生体医工学会　専門別研究会　医療⽤トランスデューサデザイン研究会

2023.04 - Now
幹事生体医工学会専門別研究会医療用トランスデューサデザイン研究会

2020.04 - 2023.03
「論文委員会（Ｅグループ）」幹事一般社団法人　電気学会

2018.04 - 2019.03
第35回センサ・マイクロマシンと応用システムシンポジウム論文委員会委員一般社団法人　電気学会

2018.04 - 2019.03
専門調査員文部科学省科学技術・学術政策研究所（NISTEP）科学技術予測・政策基盤調査研究センター

2017.04 - Now
「第33回センサ・マイクロマシンと応用システムシンポジウム論文委員会」委員一般社団法人　電気学会

2016.04 - 2017.03
論文委員会　幹事一般社団法人　電気学会

2016.04 - 2017.03
「第33回センサ・マイクロマシンと応用システムシンポジウム論文委員会」委員一般社団法人　電気学会

2015.04 - 2016.03
日本分析化学会近畿支部　常任幹事日本分析化学会

2014.04 - Now
論文編集委員化学とマイクロ・ナノシステム学会

2013.04 - 2018.03

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Awards

「第5回ネイチャー・インダストリー・アワード―若手研究者からの発信―」特別賞

2016.11 大阪科学技術センター

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Country：Japan
2014年度「貴金属に関わる研究助成金制度」MMS賞

2015.03 田中貴金属工業

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Country：Japan
「第2回ネイチャー・インダストリー・アワード―若手研究者からの発信―」技術開発委員会賞

2013.11 大阪科学技術センター

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Country：Japan
日本分析化学会2013年度奨励賞

2013.09 日本分析化学会

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Country：Japan
第4回　ひがしんビジネス大賞

2012.10 大阪東信用金庫

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Country：Japan
第32回　応用物理学会講演奨励賞

2012.09 応用物理学会

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Country：Japan
平成23年度　化学とマイクロ・ナノシステム学会　若手優秀賞

2012.05 化学とマイクロ・ナノシステム学会

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Country：Japan
第2回化学センサー・バイオセンサーおよび計測技術合同ワークショップ　優秀賞

2008.09 日本分析化学会化学センサー研究懇談会

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Country：Japan
第10回「貴金属に関わる研究助成金制度」金賞

2008.09 田中貴金属工業

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Country：Japan
第9回「貴金属に関わる研究助成金制度」MMS賞

2007.09 田中貴金属工業

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Country：Japan
第8回「貴金属に関わる研究助成金制度」MMS賞

2006.08 田中貴金属工業

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Country：Japan
日本化学会第85春季年会　学生講演賞

2005.05 日本化学会

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Country：Japan
「第5回ネイチャー・インダストリー・アワード―若手研究者からの発信―」特別賞

遠藤達郎

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Job Career (off-campus)

大阪公立大学大学院工学研究科物質化学生命系専攻

2022.04 - Now
文部科学省　科学技術・学術政策研究所　科学技術予測センター　専門調査員

2017.04 - Now
科学技術振興機構さきがけ研究員

2016.10 - 2020.03
大阪大学　大学院医学系研究科　招聘准教授

2015.04 - 2017.03
Osaka Prefecture University Graduate School of Engineering

2011.04 - Now
東京工業大学大学院　総合理工学研究科　メカノマイクロ工学専攻　助手・助教

2006.04 - 2011.03

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Papers

Assessment of the structural change of DNA by binding with a small molecule based on capillary sieving electrophoresis.

Masahide Wada, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 2024.02（ ISSN:0910-6340 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

In this study, capillary sieving electrophoresis (CSE) using polymer solutions was used to evaluate the structural changes in nucleic acids upon complexation with small molecules. As the model target and nucleic acids, L-tyrosinamide (Tyr-Am) and its aptamer, which is a type of DNA specifically binding to Tyr-Am, were selected. CSE was conducted using a capillary filled with background solution (BGS) containing hydroxypropyl cellulose (HPC) as a sieving matrix. When Tyr-Am or tyrosine was added to the BGS in CSE, the ratio of mobility differences of the Tyr-Am-aptamer complex increased compared to that of the free aptamer without the addition of Tyr-Am. In contrast, when other amino acids or their analogs were added, results showed no apparent change or decreases in electrophoretic mobility. These results indicate that the proposed method can be applied to assess structural changes in nucleic acids that target small molecules.

DOI： 10.1007/s44211-024-00524-8

PubMed
Water detection in organic solvents using a copolymer membrane immobilised with a fluorescent intramolecular charge transfer-type dye: effects of intramolecular hydrogen bonds.

Ami Morimoto, Kei Shimizu, Naoya Suzuki, Shigeyuki Yagi, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

The Analyst 149 ( 6 ) 1939 - 1946 2024.02（ ISSN:0003-2654 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Numerous fluorescent dye-based optical sensors have been developed to detect water in organic solvents. However, only a few such sensors can detect water in polar solvents such as methanol or dimethyl sulfoxide, and their detection range is generally narrow. Therefore, in this study, a copolymer membrane incorporated with a pyridinium betaine dye (denoted PB1), which exhibited intramolecular charge transfer (ICT) characteristics, was developed to realise simple water detection in organic solvents. The pyridinium betaine structure, comprising intramolecular hydrogen bonds between the oxygen in the maleimide moiety and the hydrogen in the pyridinium, was vital for achieving efficient fluorescence emission. The membrane was prepared by copolymerising PB1 with the N,N-dimethyl acrylamide/acrylamide monomer on a glass plate, and the fluorescence in water-mixed organic solvents was investigated (λabs = 490 nm, λfl = 630 nm). The fluorescence intensity of the dye-immobilised membrane decreased with increasing water content of the organic solvents. The detection ranges in tetrahydrofuran, ethanol, methanol, and dimethyl sulfoxide were approximately <40, <40, <40, and <60 vol% water, respectively. In contrast, membranes based on a quaternary pyridinium dye (without intramolecular hydrogen bonds) did not detect water in methanol and dimethyl sulfoxide, although it was more sensitive than PB1 in the narrow region of low water concentration in THF. Theoretical calculations corroborated the importance of the pyridinium betaine structure in detecting water in organic solvents, with the increase in polarity and the formation of intermolecular hydrogen bonds between PB1 and water found to induce molecular rotation and fluorescence quenching.

DOI： 10.1039/d3an02165c

PubMed
Development of a Cartridge Device Integrated with Multiple Hydrogels Exhibiting Different Functionalities for Two-dimensional Digital Electrophoresis

KANAOKA Tadamasa, ENDO Tatsuro, HISAMOTO Hideaki, SUEYOSHI Kenji

BUNSEKI KAGAKU 72 ( 9 ) 369 - 375 2023.09（ ISSN:05251931 ）

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A two-dimensional electrophoresis method, involving involves isoelectric focusing in the first dimension and polyacrylamide gel electrophoresis in the second dimension, is commonly used for the separation and analysis of protein mixtures. However, conventional methods have some drawbacks such as complex experimental procedures, long analysis time, and high consumption of reagents. In this study, based on the knowledge gained from our previous research on digital electrophoresis, a cartridge device with heterogeneous functionalized gels arranged and integrated in a two-dimensional pattern was used. The fundamental evaluation using model proteins confirmed the feasibility of sample introduction using the developed devices, digital sieving electrophoresis, and digital isoelectric focusing using pH-buffered gels. Furthermore, the verification of two-dimensional digital electrophoresis using a cartridge device with multiple pH-buffered gels and molecular sieving gels demonstrated continuous two-dimensional digital separation by employing the single device.

DOI： 10.2116/bunsekikagaku.72.369
【"微"の液相分離分析】二次元デジタル電気泳動分離のための異種機能性ハイドロゲル集積カートリッジデバイスの開発

金岡忠政, 遠藤達郎, 久本秀明, 末吉健志

分析化学 72 ( 9 ) 369 - 375 2023.09（ ISSN:0525-1931 ）

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タンパク質混合試料の分離分析法として,一次元目に等電点電気泳動,二次元目にポリアクリルアミドゲル電気泳動を行う二次元電気泳動法が一般的に利用されている.しかしながら,従来法では煩雑な実験操作と長い分析時間を要するほか,多量の試薬を消費する点が課題であった.そこで本研究では,これまでに開発してきたデジタル電気泳動に関する知見を基に,異種機能性ゲルが二次元的に配置・集積された平板型カートリッジデバイスを開発した.モデルタンパク質を用いた基礎評価の結果,作製したデバイスと治具を用いた試料導入,デジタル分子ふるい電気泳動,及びpH緩衝ゲルを用いたデジタル等電点電気泳動がそれぞれ可能であることが確認された.さらに,複数のpH緩衝ゲル及び分子ふるいゲルが配置・集積されたカートリッジデバイスを用いた二次元デジタル電気泳動について検証した結果,同一デバイス内での連続的二次元デジタル分離が実証された.(著者抄録)
Dielectrophoretic trapping of nanosized biomolecules on plasmonic nanohole arrays for biosensor applications: simple fabrication and visible-region detection

Satoko Fujiwara, Misaki Hata, Ikumi Onohara, Daiki Kawasaki, Kenji Sueyoshi, Hideaki Hisamoto, Masato Suzuki, Tomoyuki Yasukawa, Tatsuro Endo

RSC Advances 13 ( 31 ) 21118 - 21126 2023.07（ eISSN:2046-2069 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Surface plasmon resonance is an optical phenomenon that can be applied for label-free, real-time sensing to directly measure biomolecular interactions and detect biomarkers in solutions. Previous studies using plasmonic nanohole arrays have monitored and detected various biomolecules owing to the propagating surface plasmon polaritons (SPPs). Extraordinary optical transmission (EOT) that occurs in the near-infrared (NIR) and infrared (IR) regions is usually used for detection. Although these plasmonic nanohole arrays improve the sensitivity and throughput for biomolecular detection, these arrays have the following disadvantages: (1) molecular diffusion in the solution (making the detection of biomolecules difficult), (2) the device fabrication's complexities, and (3) expensive equipments for detection in the NIR or IR regions. Therefore, there is a need to fabricate plasmonic nanohole arrays as biomolecular detection platforms using a simple and highly reproducible procedure based on other SPP modes in the visible region instead of the EOT in the NIR or IR regions while suppressing molecular diffusion in the solution. In this paper, we propose the combination of a polymer-based gold nanohole array (Au NHA) obtained through an easy process as a simple platform and dielectrophoresis (DEP) as a biomolecule manipulation method. This approach was experimentally demonstrated using SPP and LSPR modes (not EOT) in the visible region and simple, label-free, rapid, cost-effective trapping and enrichment of nanoparticles (trapping time: <50 s) and bovine serum albumin (trapping time: <1000 s) was realized. These results prove that the Au NHA-based DEP devices have great potential for real-time digital and Raman bioimaging, in addition to biomarker detection.

DOI： 10.1039/d3ra03245k

PubMed
Investigation of Probe DNA Immobilization Method for Label-free DNA Biosensing Using Polymer-based Photonic Crystal

Ryosuke Nishitsuji, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

Sensors and Materials 35 ( 3 ) 1035 - 1035 2023.03（ ISSN:0914-4935 ）（ eISSN:2435-0869 ）

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Publishing type：Research paper (scientific journal)

DOI： 10.18494/sam4329
Single Cycle Selection of CD63-targeting Aptamers Using a Microscale Electrophoretic Filtration Device

TOBITA Arisa, TAKAO Junku, ENDO Tatsuro, HISAMOTO Hideaki, SUEYOSHI Kenji

BUNSEKI KAGAKU 72 ( 3 ) 111 - 116 2023.03（ ISSN:05251931 ）

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Aptamers, which show properties of specific binding to their target molecules, are expected to become an alternative to antibodies for use as molecular recognition probes instead of antibodies. However, long and laborious selection procedures have hindered their application. To overcome the drawbacks, rapid and simple selection methods using a microscale electrophoretic filtration device have been developed. In the first study, the aptamers for immunoglobulin E were successfully selected by three cycle selection based on the developed method. To realize more rapid and simple selection, aptamer selection by single cycle procedure was demonstrated. It was confirmed that CD63 as a model target was successfully trapped by the capillary device partially plugged with a polyacrylamide hydrogel. A DNA library containing various sequences were then electrokinetically introduced into the device after trapping CD63, a large volume of DNAs bound with the trapped CD63. After washing and elution schemes, DNAs located in the eluents were enhanced by PCR, and then their sequences were analyzed by next generation sequencer. As a result, one of the obtained aptamer candidates shows similar motifs to the previously reported aptamer. Isothermal titration calorimetry verified the binding ability and specificity of the obtained candidate to CD63. Consequently, it was clarified that the developed method and device is applicable to the rapid and simple selection of aptamers using the single cycle procedure.

DOI： 10.2116/bunsekikagaku.72.111
【ナノ・マイクロ分析化学の新展開】ミクロスケール電気泳動フィルタリングデバイスを用いるCD63標的アプタマーの1サイクル選抜

飛田安梨沙, 高尾隼空, 遠藤達郎, 久本秀明, 末吉健志

分析化学 72 ( 3 ) 111 - 116 2023.03（ ISSN:0525-1931 ）

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標的分子に対する特異的結合能を示す配列を持つ核酸であるアプタマーは,抗体に代わる分子認識素子としての応用が期待される分子である.しかしながら,従来のアプタマー選抜法では煩雑な実験操作を10～15サイクル繰り返す必要があるため,アプタマー関連研究におけるボトルネックとなってしまっている.本研究では,これまでに開発したミクロスケール電気泳動フィルタリングに基づくアプタマー選抜法を細胞外小胞マーカー(CD63)のアプタマー選抜へと応用した.その結果,簡便な電気泳動に基づく標的分子導入,核酸導入,結合した核酸の洗浄・回収からなるわずか1サイクルの選抜操作によって,CD63アプタマー候補が獲得された.また,等温滴定カロリメトリーの結果から,選抜されたアプタマー候補の一つがCD63に対する結合能を持ち,非標的分子のウシ血清アルブミンには応答しないことが確認された.(著者抄録)
研究室だより：大阪公立大学　大学院工学研究科　物質化学生命系専攻　分析化学研究グループ

遠藤達郎

電気学会論文誌Ｅ（センサ・マイクロマシン部門誌） 143 ( 1 ) NL1_1 - NL1_1 2023.01（ ISSN:13418939 ）（ eISSN:13475525 ）

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DOI： 10.1541/ieejsmas.143.nl1_1
Combining TiO<inf>2</inf> photonic crystals and reagents enhances the sensitivity of endotoxin detection

Nishitsuji R.

Chemical Papers 77 ( 1 ) 611 - 615 2023.01（ ISSN:03666352 ）

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DOI： 10.1007/s11696-022-02485-6
Nanoemulsion-based silver ion-selective optode based on colorimetrically silver ion-responsive ionic liquid-based dye.

Shuto Oka, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 39 ( 8 ) 1249 - 1256 2023（ ISSN:09106340 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

In this study, we describe the fast-responsive nanoemulsion (NE)-based silver ion (Ag+)-selective optode based on colorimetrically silver ion-responsive ionic liquid-based dye (ILD). The ILD comprises purely functional sensing molecules, a protonated cationic merocyanine dye (KD-M13-H+) and an anionic Ag+ ionophore (BDM-SO3-), and thus, it can be used for highly sensitive silver ion (Ag+) sensing due to the extremely high content of dye in the organic phase (ionic-liquid phase). However, during the Ag+ sensing, the cationic merocyanine dye is converted into electrically neutral form by deprotonation of the dye, which leads to the conversion of liquified dye into solid form in the organic phase, which makes the response time slower when ILD is used for poly(vinyl chloride) (PVC) membrane-based ion-selective optode, especially for sensing of high Ag+ concentration. To solve this problem, we focused on the use of the nano-emulsification technique. The response time of the ILD-based nanoemulsion (NE) was considerably shorter (1 s) compared to that of the ILD-based PVC membrane (a few minutes) owing to the large surface area and excellent diffusivity of the emulsion. The ILD-based NE contained a very high dye concentration (833 mmol kg-1) and exhibited approximately 12 times higher sensitivity than that of the plasticizer-based conventional NE. In the cation measurements, the ILD-based NE responded to Ag+ via a cation-exchange mechanism and demonstrated a highly selective response to Ag+ (log [Formula: see text] = - 3.0). ILD-NE was successfully applied to the detection of spiked Ag+ in a tap water sample with recoveries of 98 - 103% with a relative standard deviation (RSD) of less than 5%. In comparison with NE based on non-ionic ionophores without charge, NE based on BDM-SO3- responded to lower Ag+ concentrations owing to the effect of negative charge on the binding property. The novel ILD-based NE was capable of highly sensitive, rapid, and selective Ag+ sensing, providing potential for analytical devices applicable to high-performance on-site analysis.

DOI： 10.1007/s44211-023-00337-1

PubMed
ナノフォトニクスを基盤技術とした生体情報計測

遠藤達郎

生体医工学 Annual61 ( Abstract ) 133_2 - 133_2 2023（ ISSN:1347443X ）（ eISSN:18814379 ）

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生体の化学情報を取得することが可能なバイオセンサは、将来の医療・創薬・看護等様々な分野において応用が期待されるデバイスであり、我々は、ナノメートルの構造より観察される光学特性を利活用する学術領域である「ナノフォトニクス」を基盤としたバイオセンサ開発を行っている。ナノメートルの構造より発現される光学特性は、バルク状態とは異なり、特定波長の光吸収・回折・干渉等を観察することができる。加えて観察される光学特性は、周辺の屈折率変化に対して鋭敏であるため、抗原抗体反応やDNAハイブリダイゼーション等種々の生化学反応によって誘起される屈折率変化を高感度に検出、生体の化学情報を取得可能なバイオセンサ開発が期待できる。我々は、ナノメートルサイズの構造が結晶様に周期的に配列した光学素子であるフォトニック結晶やプラズモニック結晶を用いたバイオセンサ開発を行ってきた。本発表では、前述したバイオセンサを用いた生体の化学情報取得への応用について紹介する。

DOI： 10.11239/jsmbe.annual61.133_2
Aptamer Selection Based on Microscale Electrophoretic Filtration Using a Hydrogel-Plugged Capillary Device.

Junku Takao, Reina Nagai, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi

Molecules (Basel, Switzerland) 27 ( 18 ) 2022.09

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

This study reports a novel aptamer selection method based on microscale electrophoretic filtration. Aptamers are versatile materials that recognize specific targets and are attractive for their applications in biosensors, diagnosis, and therapy. However, their practical applications remain scarce due to issues with conventional selection methods, such as complicated operations, low-efficiency separation, and expensive apparatus. To overcome these drawbacks, a selection method based on microscale electrophoretic filtration using a capillary partially filled with hydrogel was developed. The electrophoretic filtration of model target proteins (immunoglobulin E (IgE)) using hydrogel, the electrokinetic injection of DNAs to interact with the trapped proteins, the elimination of DNAs with weak interactions, and the selective acquisition of aptamer candidates with strong interactions were successfully demonstrated, revealing the validity of the proposed concept. Two aptamer candidates for IgE were obtained after three selection cycles, and their affinity for the target was confirmed to be less than 1 nM based on their dissociation constant (KD) values. Therefore, the proposed method allows for the selection of aptamers with simple operations, highly effective separation based on electrophoresis and filtration, and a relatively cheap apparatus with disposable devices.

DOI： 10.3390/molecules27185818

PubMed
Fabrication of Gold Nanostructures on Quartz Crystal Microbalance Surface Using Nanoimprint Lithography for Sensing Applications.

Ryosuke Nishitsuji, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

Micromachines 13 ( 9 ) 2022.08（ ISSN:2072-666X ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

A quartz crystal microbalance (QCM) is a sensor that uses the piezoelectric properties of quartz crystals sandwiched between conductive electrodes. Localized surface plasmon resonance (LSPR) is an analytical technique that uses the collective vibration of free electrons on metal surfaces. These measurements are known as analysis techniques that use metal surfaces and have been applied as biosensors because they allow for the label-free monitoring of biomolecular binding reactions. These measurements can be used in combination to analyze the reactions that occur on metal surfaces because different types of information can be obtained from them. However, as different devices are used for these measurements, the results often contain device-to-device errors and are not accurately evaluated. In this study, we directly fabricated gold nanostructures on the surface of a QCM to create a device that can simultaneously measure the mass and refractive index information of the analyte. In addition, the device could be easily fabricated because nanoimprint lithography was used to fabricate gold nanostructures. As a proof of concept, the nanoparticle adsorption on gold nanostructures was evaluated, and it was observed that mass and refractive index information were successfully obtained without device-to-device errors.

DOI： 10.3390/mi13091430

PubMed
Gold Nanocone Array with Extensive Electromagnetic Fields for Highly Reproducible Surface-Enhanced Raman Scattering Measurements.

Satoko Fujiwara, Daiki Kawasaki, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

Micromachines 13 ( 8 ) 2022.07（ ISSN:2072-666X ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Surface-enhanced Raman scattering (SERS) is a technique used to distinguish the constitution of disease-related biomarkers in liquid biopsies, such as exosomes and circulating tumor cells, without any recognition elements. Previous studies using metal nanoparticle aggregates and angular nanostructures have achieved the detection of various biomarkers owing to strong hot spots and electromagnetic (EM) fields by localized surface plasmon resonance (LSPR). Although these SERS platforms enable significant enhancement of Raman signals, they still have some problems with the fabrication reproducibility of platforms in obtaining reproducible SERS signals. Therefore, highly reproducible fabrication of SERS platforms is required. Here, we propose the application of a polymer-based gold (Au) nanocone array (Au NCA), which extensively generates an enhanced EM field near the Au NCA surface by LSPR. This approach was experimentally demonstrated using a 785 nm laser, typically used for SERS measurements, and showed excellent substrate-to-substrate reproducibility (relative standard deviation (RSD) < 6%) using an extremely simple fabrication procedure and very low laser energy. These results proved that a Au NCA can be used as a highly reproducible SERS measurement to distinguish the constitution of biomarkers.

DOI： 10.3390/mi13081182

PubMed
Quantum dots / TiO2 hybrid photonic crystal: Fabrication and application for highly sensitive and visible region-responsive biosensor

Shotaro Ueda, Daiki Kawasaki, Ryosuke Nishitsuji, Hirotaka Yamada, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

MICROELECTRONIC ENGINEERING 263 2022.07（ ISSN:0167-9317 ）（ eISSN:1873-5568 ）

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Publishing type：Research paper (scientific journal)

In this work, by combining a photonic crystal (PhC) and highly luminescent material, we developed an extremely sensitive PhC sensor that allows detection by single wavelength fluorescence intensity change in the visible wavelength region. We fabricated a titanium dioxide (TiO2) PhC containing quantum dots (QDs) inside the PhC structure and confirmed that the fluorescence intensity of the QDs was enhanced. Fluorescence enhancement occurs because of the total reflection and light confinement of the QD fluorescence inside the TiO2 PhC structure. The response to changes in the surrounding refractive index and protein adsorption was confirmed. Furthermore, for biosensing applications, troponin T (TnT), an acute myocardial marker, was determined using an antigen -antibody reaction. It was found that a TnT level of 100 fg/mL, which is considerably less than the cutoff value of 100 pg/mL, was successfully detected. The lower limit of detection (LOD) was found to be 58.2 fg/mL, which is 1/40 of that of the conventional enzyme-linked immunosorbent assay (ELISA) method (LOD: 2 pg/mL). It was demonstrated that the present TiO2 PhC-containing QDs can be used as a sensor device for the highly sensitive detection and determination of disease biomarkers.

DOI： 10.1016/j.mee.2022.111842
Development of Capillary Devices for Digital Molecular Sieving Electrophoresis

SUEYOSHI Kenji, MATSUDA Keita, ENDO Tatsuro, HISAMOTO Hideaki

BUNSEKI KAGAKU 71 ( 6 ) 325 - 331 2022.06（ ISSN:05251931 ）

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Recently, digital electrophoresis has attracted attention as a separation and preconcentration method based on the passage or non-passage of sample molecules through various functional hydrogels. However, the complex fabrication of the device filled with various hydrogels has been a major issue. In this study, an easy-to-fabricate capillary device for digital electrophoresis is proposed and developed by the successive introduction of prepolymer solutions. Digital molecular sieving electrophoresis using polyacrylamide hydrogels with different densities was employed for a fundamental characterization of the developed device. As a result, the correlation between the total concentration of acrylamide species in the prepolymer solution (%T) and the molecular weights of the passing or non-passing proteins was clarified. The evaluation of the concentration efficiency showed that the sensitivity increased up to about 180 times. In addition, digital molecular sieve electrophoresis using the device successively plugged with 5, 15, and 25 %T polyacrylamide hydrogels achieved simultaneous separation and preconcentration of proteins with different molecular weights. These results demonstrate high-resolution with high-sensitivity digital electrophoresis using the simply fabricated device.

DOI： 10.2116/bunsekikagaku.71.325
【高感度解析に寄与する分離分析技術】デジタル分子ふるい電気泳動用キャピラリーデバイスの開発

末吉健志, 松田景太, 遠藤達郎, 久本秀明

分析化学 71 ( 6 ) 325 - 331 2022.06（ ISSN:0525-1931 ）

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デジタル電気泳動法は、各種機能性ヒドロゲルに対する試料分子の通過・非通過を利用した分離・濃縮法として近年注目されているが、ヒドロゲル充填デバイス作製の煩雑さが大きな課題であった。そこで、本研究では、簡易デバイスを用いたデジタル電気泳動技術の開発と、機能性ヒドロゲルによるタンパク質の分離・濃縮評価のため、異種ポリアクリルアミドゲル連続充填キャピラリーデバイスを作製し、デジタル分子ふるい電気泳動の基礎評価を行った。その結果、プレポリマー溶液中アクリルアミド総濃度(%T)と通過・非通過タンパク質の分子量との相関が明らかとなった。また、濃縮効率評価の結果、最大で約180倍の高感度化が確認された。さらに、5、15、25%Tポリアクリルアミドゲル連続充填デバイスを用いたデジタル分子ふるい電気泳動によって、分子量が異なるタンパク質の分離と濃縮が同時に達成された。以上の結果から、作製したデバイスを用いた簡便・高分離能・高感度なデジタル電気泳動が実証された。(著者抄録)
Wavelength-tunable dual-band edge plasmon mode based on gold edge-hole plasmonic nanostructure

Hirotaka Yamada, Daiki Kawasaki, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

RESULTS IN PHYSICS 37 2022.06（ ISSN:2211-3797 ）

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Publishing type：Research paper (scientific journal)

Metal nanostructures have attracted attention because of their unique optical characteristics derived from localized surface plasmon resonance. In this study, gold nanoholes with edge structures were designed and characterized to possess two plasmon bands at different wavelengths. These bands were assigned to the plasmon modes that generated a concentrated electric field at the tip of the individual edge structures. Furthermore, wavelengths of these bands were independently tuned from the visible to near-infrared wavelengths with shape and size of the gold nanoholes and edge structures as variables. To demonstrate wavelength tuning, a gold nanostructure with edge plasmon bands at wavelengths of approximately 785 and 1064 nm (typical laser wavelengths for surface-enhanced Raman scattering and optical tweezers, respectively) was successfully obtained via optical simulation. The results indicate that the structure designed in this study provides a guideline for the design of optical devices with efficient multiple optical functions.

DOI： 10.1016/j.rinp.2022.105541
Evaluation of the interactions between oligonucleotides and small molecules by partial filling-nonequilibrium affinity capillary electrophoresis.

Eriko Mitsuno, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 38 ( 6 ) 851 - 859 2022.06（ ISSN:0910-6340 ）（ eISSN:1348-2246 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Recently, high-throughput analysis with minimal reagent consumption has been desired to assess interactions between drug candidates and disease-related oligonucleotides. To realize an ideal assay for drug screening, a rapid assay based on affinity capillary electrophoresis was generated to reduce the consumption of samples/reagents by a partial-filling technique under nonequilibrium conditions. In the proposed method, the first sample, oligonucleotide as a ligand, and second sample zones were injected into a capillary with spacers of background solution between the samples and oligonucleotide zones. After applying voltages, only the second sample zone passed through the partially filled oligonucleotide zone, resulting in variations in the peak parameters owing to this interaction. The electropherograms obtained were analyzed using equilibrium, reaction kinetics, and moment theories. In the interaction analyses between small molecules and DNA aptamers, only the small molecules binding to the aptamer showed significant changes in their peak heights and shapes. The estimated kinetic parameters were in good agreement with the reported values, indicating the applicability of the proposed method for drug screening. When interactions between drug candidates and disease-related RNAs were analyzed, one of the candidates showed remarkable variation in the peak parameters upon the addition of potassium ions. Consequently, the proposed method could be one of the ideal assays for drug screening.

DOI： 10.1007/s44211-022-00101-x

PubMed
Chloride ion-selective dye liquid nanoemulsion: improved sensor performance due to intermolecular interactions between dye and ionophore.

Kaho Maki, Ryoutarou Oishi, Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

The Analyst 147 ( 8 ) 1529 - 1533 2022.04（ ISSN:0003-2654 ）（ eISSN:1364-5528 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Ionophore-based dye liquid nanoemulsion sensors exhibiting rapid response, high selectivity, and high sensitivity to chloride were developed. Since nanoemulsions contain extremely high concentrations of dyes and have large surface areas, rapid and highly sensitive measurements were possible. 1H-NMR measurements revealed intermolecular interactions between the dye and the ionophore, which contributed to the suppression of the background signal.

DOI： 10.1039/d2an00115b

PubMed
Imprinted Photonic Crystal-Film-Based Smartphone-Compatible Label-Free Optical Sensor for SARS-CoV-2 Testing.

Daiki Kawasaki, Hirotaka Yamada, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

Biosensors 12 ( 4 ) 2022.03（ eISSN:2079-6374 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

The coronavirus disease (COVID-19) caused by SARS-CoV-2 has caused a global pandemic. To manage and control the spread of the infection, it is crucial to develop and implement technologies for the early identification of infected individuals and rapid informatization in communities. For the realization of such a technology, a widely available and highly usable sensor for sensitive and specific assay of the virus plays a fundamental role. In this study, we developed an optical sensor based on an imprinted photonic crystal film (IPCF) for quick, simple, and cost-effective detection of SARS-CoV-2 spike protein in artificial saliva. Our IPCF sensor enabled label-free and highly sensitive detection with a smartphone-equipped optical setup. The IPCF surface was functionalized with an anti-SARS-CoV-2 spike protein antibody for immunoassay. We evaluated the specificity and sensitivity of the IPCF sensor for quantitative detection of the spike protein in artificial saliva using simple reflectometry with a spectrometer-equipped optical setup. Specific and quantitative detection of the spike protein was successfully achieved, with a low detection limit of 429 fg/mL. In the demonstration of reflectometric detection with a smartphone-equipped setup, the sensitivity was comparable with that with a spectrometer-equipped setup. The test result is returned immediately and can be saved to cloud storage. In addition, it costs less than USD 1 for one IPCF to be used for diagnosis. Thus, the developed IPCF has the potential to realize a widely available and highly usable sensor.

DOI： 10.3390/bios12040200

PubMed
Fabrication of Metal-Insulator-Metal Nanostructures Composed of Au-MgF2-Au and Its Potential in Responding to Two Different Factors in Sample Solutions Using Individual Plasmon Modes.

Hirotaka Yamada, Daiki Kawasaki, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

Micromachines 13 ( 2 ) 2022.02（ eISSN:2072-666X ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

In this paper, metal-insulator-metal (MIM) nanostructures, which were designed to exhibit two absorption peaks within 500-1100 nm wavelength range, were fabricated using magnesium difluoride (MgF2) as the insulator layer. Since the MIM nanostructures have two plasmon modes corresponding to the absorption peaks, they independently responded to the changes in two phases: the surrounding medium and the inside insulator layer, the structure is expected to obtain multiple information from sample solution: refractive index (RI) and molecular interaction between solution components and the insulator layer. The fabricated MIM nanostructure had a diameter of 139.6 ± 2.8 nm and a slope of 70°, and exhibited absorption peaks derived from individual plasmon modes at the 719 and 907 nm wavelengths. The evaluation of the response to surrounding solution component of the MIM nanostructures revealed a linear response of one plasmon mode toward the RI of the surrounding medium and a large blue shift of the other plasmon mode under conditions where glycerol was present at high concentration. From optical simulation and the evaluation of the MgF2 fabricated by deposition, the blue shift was expected to be due to the swelling of MgF2 interacting with the hydroxyl groups abundantly included in the glycerol molecules. The results indicated the individual responses of two plasmon modes in MIM nanostructures toward medium components, and brought the prospect for the simultaneous measurement of multiple elements using two or more plasmon modes.

DOI： 10.3390/mi13020257

PubMed
ナノ光学素子を用いた非標識バイオセンシング

遠藤達郎

生体医工学 Annual60 ( Abstract ) 104_2 - 104_2 2022（ ISSN:1347443X ）（ eISSN:18814379 ）

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　ナノメートルサイズの構造は、特定波長の光吸収・回折・干渉といったバルク状態とは異なる特異的な光学特性を観察することが可能である。加えて観察される光学特性は、周辺の屈折率変化に対して鋭敏であることから、抗原抗体反応やDNAハイブリダイゼーション等種々の生化学反応によって誘起される屈折率変化を高感度に検出可能なセンサへの応用が期待できる。　我々は、ナノメートルサイズの構造が結晶様に周期的に配列した光学素子であるフォトニック結晶に着目し、バイオセンサへの応用を行ってきた。フォトニック結晶は、周期や周辺屈折率分布が変化に依存した特定波長光を回折・反射させる特徴を有する。またフォトニック結晶をバイオセンサへと応用するには、抗原抗体反応等種々の生化学反応によって誘起される周辺屈折率変化を光回折・反射ピーク/強度変化として測定するものである。　我々は、ポリマーを基材としてフォトニック結晶を作製することに成功し、各種疾病に関連するマーカー分子を検出・定量するバイオセンサ開発に成功してきたが、可視領域中において屈折率の低い材料であるポリマーを基材としてフォトニック結晶を作製し、バイオセンサへと応用するには検出可能な濃度域の改善は困難という課題があった。前述した課題を解決するため、本研究では可視領域中において高い屈折率を有する基材を用いてフォトニック結晶を作製し、バイオセンサへと応用したので報告する。

DOI： 10.11239/jsmbe.annual60.104_2
Development of Capillary Devices for Digital Molecular Sieving Electrophoresis

Kenji Sueyoshi, Keita Matsuda, Tatsuro Endo, Hideaki Hisamoto

BUNSEKI KAGAKU 71 ( 6 ) 325 - 331 2022（ ISSN:0525-1931 ）

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Recently, digital electrophoresis has attracted attention as a separation and preconcentration method based on the passage or non-passage of sample molecules through various functional hydrogels. However, the complex fabrication of the device filled with various hydrogels has been a major issue. In this study, an easy-to-fabricate capillary device for digital electrophoresis is proposed and developed by the successive introduction of prepolymer solutions. Digital molecular sieving electrophoresis using polyacrylamide hydrogels with differcni densities was employed for a fundamental characterization of the developed device. As a result, the correlation between the total concentration of acrylamide species in the prepolymer solution (%T) and the molecular weights of the passing or non-passing proteins was clarified. The evaluation of the concentration efficiency showed that the sensitivity increased up to about 180 times. In addition, digital molecular sieve electrophoresis using the device successively plugged with 5, 15, and 25 %T polyacrylamide hydrogels achieved simultaneous separation and preconcentration of proteins with different molecular weights. These results demonstrate high-resolution with high-sensitivity digital electrophoresis using the simply fabricated device.

J-GLOBAL
Au nanorods-TiO2 photonic crystal plasmonic-photonic hybrid sensor for label-free detection and identification of DNA molecules with single nucleotide polymorphisms Reviewed

Daiki Kawasaki, Hirotaka Yamada, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

SENSORS AND ACTUATORS B-CHEMICAL 361 131747 - 131747 2022（ eISSN:0925-4005 ）

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Publishing type：Research paper (scientific journal)

Highly sensitive optical sensors for label-free detection of DNA must have high usability and wide applicability for achieving advances in the medical and biological science fields. However, current sensors do not simultaneously satisfy these criteria. Here, we developed a simple, cost-effective, and sensitive plasmonic-photonic hybrid sensor using gold nanorods as nanoantennae and a photonic crystal slab (PCS) as a microcavity. In this report, we performed simple microscopic detection and identification of DNA molecules with Alzheimer's disease-associated single nucleotide polymorphisms, which is an important indicator for early diagnosis. The PCS was fabricated using nanoimprint lithography; the gold nanorods were controllably coupled with the PCS. The device sensitivity was experimentally assessed by controlling the plasmonic-photonic coupling resonance, which was enabled by tuning the PCS-coupled AuNR amounts. The sensitivity was also investigated by theoretical modeling of the hybrid sensor, based on the temporal coupled mode theory (TCMT). The TCMT-based theoretical model well explained the experimental results. In a demonstration of ultrasensitive detection of DNA, the identification of single nucleotide mismatched DNA molecules (similar to 1 pM) and detection of several DNA molecules (similar to 1000), with a limit of detection of 5.9 aM, were successfully achieved. This sensor may thus have wide applications for DNA analysis.

DOI： 10.1016/j.snb.2022.131747
Modulating Optical Characteristics of Nanoimprinted Plasmonic Device by Re-Shaping Process of Polymer Mold.

Hirotaka Yamada, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

Micromachines 12 ( 11 ) 2021.10（ eISSN:2072-666X ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Metal nanostructures exhibit specific optical characteristics owing to their localized surface plasmon resonance (LSPR) and have been studied for applications in various optical devices. The LSPR property strongly depends on the size and shape of metal nanostructures; thus, plasmonic devices must be designed and fabricated according to their uses. Nanoimprint lithography (NIL) is an effective process for repeatedly fabricating metal nanostructures with controlled sizes and shapes and require optical properties. NIL is a powerful method for mass-producible, low-cost, and large-area fabrication. However, the process lacks flexibility in adjusting the size and shape according to the desirable optical characteristics because the size and shape of metal nanostructures are determined by a single corresponding mold. Here, we conducted a re-shaping process through the air-plasma etching of a polymer's secondary mold (two-dimensional nanopillar array made of cyclo-olefin polymer (COP)) to modulate the sizes and shapes of nanopillars; then, we controlled the spectral characteristics of the imprinted plasmonic devices. The relationship between the structural change of the mold, which was based on etching time, and the optical characteristics of the corresponding plasmonic device was evaluated through experiments and simulations. According to evaluation results, the diameter of the nanopillar was controlled from 248 to 139 nm due to the etching time and formation of a pit structure. Consequently, the spectral properties changed, and responsivity to the surrounding dielectric environment was improved. Therefore, plasmonic devices based on the re-shaped COP mold exhibited a high responsivity to a refractive index of 906 nm/RIU at a wavelength of 625 nm.

DOI： 10.3390/mi12111323

PubMed
Direct measurement of initial rate of enzyme reaction by electrokinetic filtration using a hydrogel-plugged capillary device Reviewed

Junku Takao, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi

Analytical Sciences 雑誌 37 ( 10 ) 1439 - 1446 2021.10

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Kind of work：Joint Work
Modulating Optical Characteristics of Nanoimprinted Plasmonic Device by Re-Shaping Process of Polymer Mold Reviewed

Hirotaka Yamada, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

Micromachines 雑誌 12 ( 11 ) 2021.10

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Kind of work：Joint Work
Single-step trypsin inhibitor assay on a microchannel array device immobilizing enzymes and fluorescent substrates by inkjet printing Reviewed

Yuko Kawai, Kotaro Idegami, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

Analytical Sciences 雑誌 37 ( 10 ) 1473 - 1476 2021.10

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Kind of work：Joint Work
インクジェットプリンティングによる、マイクロチャネルアレイデバイス固定化酵素と蛍光基質を用いた1ステップトリプシンインヒビターアッセイ(Single-step Trypsin Inhibitor Assay on a Microchannel Array Device Immobilizing Enzymes and Fluorescent Substrates by Inkjet Printing)

Kawai Yuko, Ideggami Kotaro, Sueyoshi Kenji, Endo Tatsuro, Hisamoto Hideaki

Analytical Sciences 37 ( 10 ) 1473 - 1476 2021.10（ ISSN:0910-6340 ）

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本報ではインクジェットプリンティングを用いて、反応試薬を固定化したバイオアッセイマイクロデバイスによる、1ステップトリプシンインヒビターアッセイデバイスについて報告した。なお、本デバイスは、ポリジルメチルシロキサン(PDMS)製マイクロチャネルアレイで構成されており、インクジェットプリンターにより、マイクロチャネル底面に試薬をナノリットルサイズの液滴として、簡便且つ正確に位置選択的に固定化することが可能である。また、本デバイスにより、トリプシンと蛍光基質が互いに混合することなく、同じマイクロチャネル内の異なる位置にそれぞれ固定化された。さらに、トリプシン固定化のため、スポッティング剤に0.8mM HCl溶液に加えると、トリプシンの自己消化により、蛍光強度低下が抑制されることも明らかにされ、本デバイスを用いた、1ステップトリプシンインヒビタースクリーニングでは、ロイペプチンを含む3種類のトリプシンインヒビターにおいて、トリプシン阻害作用も確認された。本デバイスは10日間以上も安定して保存が可能で、位置ずれによるばらつきの問題も改善されたことからも、大量生産が期待され、本デバイスが創薬分野で、種々の酵素阻害剤アッセイに有用性が高いことが示唆された。
Enzyme-responsive fluorescent nanoemulsion based on lipophilic dye liquid

Ryoutarou Oishi, Kaho Maki, Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

ANALYST 146 ( 13 ) 4121 - 4124 2021.07（ ISSN:0003-2654 ）（ eISSN:1364-5528 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

An enzyme-responsive fluorescent nanoemulsion (NE) based on lipophilic dye liquid (LDL) was developed for alkaline phosphatase (ALP). The response mechanism of the NE involved enzymatic reactions and simultaneous extraction of anions. The LDL-based NE exhibited 3.8 times higher sensitivity than plasticizer-based conventional NE. Detection limit and response range were 2.7 (U L-1) and 5-50 (U L-1), respectively. The response time was reduced to less than half that of the LDL-based membrane.

DOI： 10.1039/d1an00447f

PubMed
Fractionation of single-stranded DNAs with/without stable preorganized structures using capillary sieving electrophoresis for aptamer selection Reviewed

Masahide Wada, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi

Analytical Sciences 雑誌 37 ( 6 ) 799 - 802 2021.06

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Kind of work：Joint Work
Fractionation of Single-stranded DNAs with/without Stable Preorganized Structures Using Capillary Sieving Electrophoresis for Aptamer Selection

Masahide Wada, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi

ANALYTICAL SCIENCES 37 ( 6 ) 799 - 802 2021.06（ ISSN:0910-6340 ）（ eISSN:1348-2246 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：Domestic journal

Aptamers, single-stranded DNAs/RNAs with a strong and specific interaction towards a target molecule, have wide applications in the fields of medicine and biosensors. In conventional aptamer selection methods, it is difficult to obtain "preorganized" and/or "induced-fit" type of aptamers selectively. In this study, separation and fractionation of single-stranded DNAs with/without stable preorganized structures were carried out using capillary sieving electrophoresis. The fractionated DNAs showed different mobilities and thermodynamic stabilities of their secondary structures; this outcome is deemed to be necessary for the synthesis of novel aptasensors with a desirable sensing mechanism.

DOI： 10.2116/analsci.21C003

PubMed
アプタマー選択のためのキャピラリー篩電気泳動法を用いた、安定的な事前に組織化した構造の有無による一本鎖DNAの分画(Fractionation of Single-stranded DNAs with/without Stable Preorganized Structures Using Capillary Sieving Electrophoresis for Aptamer Selection)

Wada Masahide, Endo Tatsuro, Hisamoto Hideaki, Sueyoshi Kenji

Analytical Sciences 37 ( 6 ) 799 - 802 2021.06（ ISSN:0910-6340 ）

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キャピラリー篩電気泳動法を用いて、安定的な事前に組織化された構造を持つ一本鎖DNAと持たない一本鎖DNAの分離・分画した。その結果、分画したDNAはその二次構造とは異なった移動度と熱力学的安定性を示した。これらの結果は、望ましい感知機序を持つ新規アプタセンサーの合成に必要と考えられた。
Enzyme-responsive fluorescent nanoemulsion based on lipophilic dye liquid Reviewed

Ryoutarou Oishi, Kaho Maki, Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

Analyst 雑誌 146 ( 13 ) 4121 - 4124 2021.05

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Kind of work：Joint Work
Single-Step Trypsin Inhibitor Assay on a Microchannel Array Device Immobilizing Enzymes and Fluorescent Substrates by Inkjet Printing.

Yuko Kawai, Kotaro Idegami, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 37 ( 10 ) 1473 - 1476 2021.04（ ISSN:0910-6340 ）（ eISSN:1348-2246 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：Domestic journal

In this paper, we report a single-step trypsin inhibitor assay on a microchannel array device immobilizing enzymes and substrates by inkjet printing. The microdevice is composed of a poly(dimethylsiloxane) (PDMS) microchannel array that immobilizes trypsin and fluorescent substrates as reactive reagents at the two bottom corners of a microchannel. Inkjet printers allow simple, accurate, and position-selective immobilization of reagents as nanoliter spots. Therefore, plural reactive reagents, such as enzymes and substrates, can be separately immobilized at different positions in the same microchannel without mixing, and thus allowing for single-step operation by simply introducing a sample solution through capillary action. Furthermore, reproducible fabrication and mass production of the device could be expected. In this study, the efficiency of an acidic solution as a spotting agent for protease immobilization to prevent decrease in the fluorescence intensity was confirmed. Additionally, single-step trypsin inhibitor screening was performed using three inhibitors. Finally, we investigated the storage stability of the device and confirmed that it remained stable for at least 10 days.

DOI： 10.2116/analsci.21N011

PubMed
Direct measurement of initial rate of enzyme reaction by electrokinetic filtration using a hydrogel-plugged capillary device.

Junku Takao, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 37 ( 10 ) 1439 - 1446 2021.04（ ISSN:0910-6340 ）（ eISSN:1348-2246 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：Domestic journal

A novel electrokinetic filtration device using a plugged hydrogel was developed to directly measure the initial rate of enzyme reactions. In the proposed method, the enzyme reaction proceeded only for a short time when the substrate was passed through a thin layer of enzyme trapped by the hydrogel without any lag times for mixing and detection. In experimental conditions, alkaline phosphatase (enzyme) was filtrated at a cathodic-side interface of the plugged hydrogel by molecular sieving effect, providing the thin enzyme zone whose thickness was approximately 100 μm. When 4-methylumberiferyl phosphate (substrate) was electrokinetically introduced into the device after trapping the enzyme, 4-methylumberiferone (product) was generated by the enzyme reaction for only 1.26 s as the substrate passed through the trapped enzyme zone. As a result, the initial rate of the enzyme reaction could be directly calculated to 31.0 μM/s by simply dividing the concentration of the product by the tunable reaction time. Compared to the initial rate obtained by mixing the enzyme and substrate solutions, the value of the maximum velocity of the enzyme reaction was 30-fold larger than that in the mixing method due to the preconcentration of the enzyme by trapping. The Michaelis-Menten constant in the proposed method was 2.7-fold larger than that in the mixing method, suggesting the variation of changes in the equilibrium of complex formation under the experimental conditions.

DOI： 10.2116/analsci.21P067

PubMed
インクジェットプリンティングによる試薬固定化法を利用する1ステップバイオアッセイマイクロデバイスの開発 Reviewed

河相優子，白井亮洋，角田正也，井手上公太郎，末吉健志，遠藤達郎，久本秀明

分析化学雑誌 70 ( 3 ) 125 - 131 2021.03

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【化学センシングの方法論・デバイス開発の新展開】インクジェットプリンティングによる試薬固定化法を利用する1ステップバイオアッセイマイクロデバイスの開発

河相優子, 白井亮洋, 角田正也, 井手上公太郎, 末吉健志, 遠藤達郎, 久本秀明

分析化学 70 ( 3 ) 125 - 131 2021.03（ ISSN:0525-1931 ）

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本研究では、インクジェットプリンティングを用いて一つのポリジメチルシロキサン(Poly(dimethylsiloxane)、PDMS)製マイクロ流路内に2種類の反応試薬を固定化した1ステップ均一系競合型バイオアッセイマイクロデバイスを開発した。インクジェットプリンターは試薬をナノリットルサイズの液滴として正確な量を位置選択的に吐出するため、互いに反応する2種類の試薬を混ざることなく微少空間内に独立して固定化できる。そのため、先行研究でデバイスを作製する際に課題となった、相互に反応する試薬を固定化した二つのマイクロ流路を精密に組み合わせる操作が不要となる。本研究ではその一例として、ビオチン固定化酸化グラフェン(BG)と蛍光標識ストレプトアビジン(F-SA)を1本のマイクロ流路内に固定してビオチンアッセイマイクロデバイスを試作し、基礎検討として試薬の固定化位置や試薬及び添加剤種の評価を行った。その結果、液滴を流路壁面寄りに吐出すると再現性よく流路底面の両サイドに試薬固定できること、添加剤としてトレハロースが適すること及び、試料導入操作のみでの1ステップ検出で、検出下限は0.68ng mL-1となり、先行研究と比較して約1/600倍改善することを明らかにした。(著者抄録)
Lipophilic Fluorescent Dye Liquids: Forster Resonance Energy Transfer-Based Fluorescence Amplification for Ion Selective Optical Sensors Based on a Solvent Polymeric Membrane

Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

ANALYTICAL CHEMISTRY 93 ( 9 ) 4143 - 4148 2021.03（ ISSN:0003-2700 ）（ eISSN:1520-6882 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Optical sensors based on solvent polymeric membranes have the potential to measure analytes present in an aqueous solution through the development of a tailored method for a specific target. However, limits in the concentrations of the component dyes have prevented improvements in sensitivity. We propose a Forster resonance energy transfer (FRET)-based fluorescence amplification system for ion-selective optical sensors using a highly fluorescent liquid material composed of a lipophilic phosphonium cation and a pyrene modifying sulfonate anion ([P-66614][HP-SO3]), as both the plasticizer and donor, in addition to a combination of the lipophilic phosphonium cation and the fluorescein dodecyl ester anion ([ P-66614][12- FL]) as the fluorescent sensing dye acceptor. For ion extraction-based sensing, the donor and acceptor were retained in the plasticized PVC membrane with negligible leaching upon exposure to acidic and basic aqueous solutions. Systematic investigation of the donor and acceptor ratios clarified the effect of the amplification factor and the sensitivity of the sensor. At an acceptor doping level of 0.5 mol % (vs donor), an approximately 22-fold higher sensitivity was obtained compared to that of a conventional PVC membrane optical sensor. During ion measurement based on the coextraction of protons and anions, selectivity following the Hofmeister order was observed, which was controlled by the addition of ionophores. The proposed FRET system based on a lipophilic fluorescent liquid material has the potential to significantly improve the sensitivities of optical sensors using solvent polymeric membranes with high selectivities for various target analytes.

DOI： 10.1021/acs.analchem.0c05007

PubMed
Lipophilic Fluorescent Dye Liquids: Förster Resonance Energy Transfer-Based Fluorescence Amplification for Ion Selective Optical Sensors Based on a Solvent Polymeric Membrane Reviewed

Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

Analytical Chemistry 雑誌 93 ( 9 ) 4143 - 4148 2021.02

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Inkjet Printing-Based Immobilization Method for a Single-Step and Homogeneous Competitive Immunoassay in Microchannel Arrays Reviewed

Yuko Kawai, Akihiro Shirai, Masaya Kakuta, Koutarou Idegami, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

Frontiers in Chemistry 雑誌 8 2020.12

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メンブレンフィルタを基材としたプラズモニックメンブレンの作製とセンサ応用 Reviewed

遠藤達郎，山田大空

電気学会論文誌Ｅ雑誌電気学会 140 ( 12 ) 2020.12

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Inkjet Printing-Based Immobilization Method for a Single-Step and Homogeneous Competitive Immunoassay in Microchannel Arrays

Yuko Kawai, Akihiro Shirai, Masaya Kakuta, Kotaro Idegami, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

FRONTIERS IN CHEMISTRY 8 612132 - 612132 2020.12（ ISSN:2296-2646 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

In this study, we report an inkjet printing-based method for the immobilization of different reactive analytical reagents on a single microchannel for a single-step and homogeneous solution-based competitive immunoassay. The immunoassay microdevice is composed of a poly(dimethylsiloxane) microchannel that is patterned using inkjet printing by two types of reactive reagents as dissolvable spots, namely, antibody-immobilized graphene oxide and a fluorescently labeled antigen. Since nanoliter-sized droplets of the reagents could be accurately and position-selectively spotted on the microchannel, different reactive reagents were simultaneously immobilized onto the same microchannel, which was difficult to achieve in previously reported capillary-based single-step bioassay devices. In the present study, the positions of the reagent spots and amount of reagent matrix were investigated to demonstrate the stable and reproducible immobilization and a uniform dissolution. Finally, a preliminary application to a single-step immunoassay of C-reactive protein was demonstrated as a proof of concept.

DOI： 10.3389/fchem.2020.612132

PubMed
Highly sensitive optical ion sensor with ionic liquid-based colorimetric membrane/photonic crystal hybrid structure Reviewed

D. Kawasaki, R. Oishi, N. Kobayashi, T. Mizuta, K. Sueyoshi, H. Hisamoto, T. Endo

Scientific Reports 雑誌 2020.10

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Template stripping method-based Au nanoarray for surface-enhanced Raman scattering detection of antiepileptic drug Reviewed

T. Endo, H. Yamada, K. Yamada

Micromachines 雑誌 2020.10

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Highly sensitive optical ion sensor with ionic liquid-based colorimetric membrane/photonic crystal hybrid structure Reviewed

Daiki Kawasaki, Ryoutarou Oishi, Nao Kobayashi, Tatsumi Mizuta, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

SCIENTIFIC REPORTS 10 ( 1 ) 16739 - 16739 2020.10（ ISSN:2045-2322 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

An ionic liquid-based thin (similar to 1 mu m) colorimetric membrane (CM) is a key nano-tool for optical ion sensing, and a two-dimensional photonic crystal slab (PCS) is an important nano-platform for ultimate light control. For highly sensitive optical ion sensing, this report proposes a hybrid of these two optical nano-elements, namely, a CM/PCS hybrid. This structure was successfully fabricated by a simple and rapid process using nanoimprinting and spin-coating, which enabled control of the CM thickness. Optical characterization of the hybrid structure was conducted by optical measurement and simulation of the reflection spectrum, indicating that the light confined in the holes of the PCS was drastically absorbed by the CM when the spectrum overlapped with the absorption spectrum of the CM. This optical property obtained by the hybridization of CM and PCS enabled drastic improvement in the absorption sensitivity in Ca ion sensing, by ca. 78 times compared to that without PCS. Experimental and simulated investigation of the relation between the CM thickness and absorption sensitivity enhancement suggested that the controlled light in the PCS enhanced the absorption cross-section of the dye molecules within the CM based on the enhanced local density of states. This highly sensitive optical ion sensor is expected to be applied for micro-scale bio-analysis like cell-dynamics based on reflectometric Ca ion detection.

DOI： 10.1038/s41598-020-73858-8

PubMed
Template Stripping Method-Based Au Nanoarray for Surface-Enhanced Raman Scattering Detection of Antiepileptic Drug Reviewed

Tatsuro Endo, Hirotaka Yamada, Kenji Yamada

MICROMACHINES 11 ( 10 ) 2020.10（ eISSN:2072-666X ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Surface-enhanced Raman scattering (SERS) is a potential candidate for highly sensitive detection of target molecules. A SERS active substrate with a noble metal nanostructure is required for this. However, a SERS active substrate requires complicated fabrication procedures. This in turn makes it difficult to fabricate highly sensitive SERS active substrates with high reproducibility. To overcome this difficulty, a plasmonic crystal (PC) with periodic noble metal nanostructures was fabricated via the template-stripping method using a polymer-based template. Using SERS active substrates, SERS was successfully achieved using the PC by detecting low concentrations of phenobarbital which is an antiepileptic drug using a commercially available portable Raman module. The PC can be fabricated by demolding the deposited gold layer from a polymer-based template. This method is rapid, economic, and has high reproducibility. SERS can be achieved easily using this PC for a wide variety of applications such as medical, pharmaceutical, and environmental protection.

DOI： 10.3390/mi11100936

PubMed
A lipophilic ionic liquid-based dye for anion optodes: importance of dye lipophilicity and application to heparin measurement Reviewed

Tatsumi Mizuta, Shu Takai, Toshiki Nishihata, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

ANALYST 145 ( 16 ) 5430 - 5437 2020.08（ ISSN:0003-2654 ）（ eISSN:1364-5528 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Herein, a fully lipophilic ionic liquid (IL) comprising a lipophilic fluorescein anion and a trihexyltetradecylphosphonium cation was synthesized and used as the plasticizer for a plasticized poly(vinyl chloride) (PVC) membrane optode. Systematic investigation of the alkyl chain length of the fluorescein anion proved the significance of lipophilicity for obtaining the reversible absorbance measurements. A PVC membrane fabricated with the synthesized lipophilic IL was observed to comprise an unusually high dye concentration (915 mmol kg(-1)) and exhibited good sensitivity as well as response time in its sensor performance. The sensitivity of the presented PVC membrane was 26-fold higher than that of a conventional optode membrane with the same membrane thickness and the same lipophilic dye of typical dye content (1 wt%). The response time was observed to be >120-fold faster by using a significantly thinner PVC membrane (approx. 140 nm). Heparin is known to be a polyanionic anticoagulant, and the presented PVC membrane exhibited an extremely fast response (20-150 seconds) to the heparin in diluted serum within the required concentration region. Thus, the lipophilic IL-based dye could significantly improve the sensor performance in conventional optodes, especially for an analyte showing slow diffusion, such as macromolecular heparin.

DOI： 10.1039/d0an00335b

PubMed
Size Sorting of Exosomes by Tuning the Thicknesses of the Electric Double Layers on a Micro-Nanofluidic Device Reviewed

Satoko Fujiwara, Kyojiro Morikawa, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi

MICROMACHINES 11 ( 5 ) 2020.05（ eISSN:2072-666X ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Exosomes, a type of extracellular vesicle with a diameter of 30-150 nm, perform key biological functions such as intercellular communication. Recently, size sorting of exosomes has received increasing attention in order to clarify the correlation between their size and components. However, such sorting remains extremely difficult. Here, we propose to sort their size by controlling their electrokinetic migration in nanochannels in a micro-nanofluidic device, which is achieved by tuning the thickness of the electric double layers in the nanochannels. This approach was demonstrated experimentally for exosomes smaller than 250 nm. Using different running buffer concentrations (1 x 10(-3), 1 x 10(-4), and 1 x 10(-5) M), most of the exosomes larger than 140, 110, and 80 nm were successfully cut off at the downstream of the nanochannels, respectively. Therefore, it is clarified that the proposed method is applicable for the size sorting of exosomes.

DOI： 10.3390/mi11050458

PubMed
Enzyme-responsive Fluorescent Ionic Liquid Reviewed

Ryoutarou Oishi, Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

ANALYTICAL SCIENCES 36 ( 2 ) 143 - 145 2020.02（ ISSN:0910-6340 ）（ eISSN:1348-2246 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：Domestic journal

Herein, we describe the development of a novel material, "enzyme-responsive fluorescent ionic liquid", which enabled a highly sensitive detection of alkaline phosphatase (ALP). We prepared a plasticized poly(vinyl chloride) (PVC) membrane using this new material as a plasticizer and quantified ALP in aqueous solutions. Preliminary results suggested that the PVC membrane responded to ALP at an interface between the membrane and the sample solution with anion extraction to maintain electroneutrality in the membrane phase. The developed PVC membrane showed an approximately six-times higher sensitivity than the conventional membrane, thereby demonstrating highly sensitive ALP detection. These results suggested the potential applicability of the proposed membrane for highly sensitive protein detection by using ALP-labeled antibodies.

DOI： 10.2116/analsci.19C013

PubMed
Angle-Sensitive Photonic Crystals for Simultaneous Detection and Photocatalytic Degradation of Hazardous Diazo Compounds Reviewed

Kenichi Maeno, Bhargav R. Patel, Tatsuro Endo, Kagan Kerman

MICROMACHINES 11 ( 1 ) 2020.01（ eISSN:2072-666X ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Congo Red (CR) and Amido Black 10B (AB-10B) are anionic diazo dyes, which are metabolized to produce a bioaccumulative and persistent carcinogen, benzidine. In this regard, an angle sensitive sensor composed of photonic crystal supported photocatalyst was fabricated for the simultaneous detection and photocatalytic degradation of diazo dyes from aqueous solutions. Reflectance spectroscopy was used in the detection of CR and AB-10B, which was based on the emergence of the incident angle dependent reflection peaks from the TiO2 coated two-dimensional photonic crystal (2D-PhC) surfaces and their subsequent quenching due to the presence of dye molecules whose absorbance peak intensity overlapped the reflection peak intensity of TiO2 at the respective angle. Interestingly, ultraviolet (UV) mediated photocatalytic degradation of CR and AB-10B was achieved using the same TiO2 coated 2D-PhC surfaces. 2D-PhC underneath the TiO2 layer was able to confine and localize the light on the TiO2 coated 2D-PhC surface, which enhanced the light absorption by dye molecules on the TiO2 surface and the photocatalytic efficiency in the degradation of CR and AB-10B. Finally, this proof-of-concept study demonstrated the fabrication of copolymer film based photonic crystal supported photocatalytic device, which can be used for developing miniaturized sensors competent in on-field detection and degradation of pollutants.

DOI： 10.3390/mi11010093

PubMed
Angle-Sensitive Photonic Crystals for Simultaneous Detection and Photocatalytic Degradation of Hazardous Diazo Compounds

K. Maeno, B. R. Patel, T. Endo, K. Kerman

Micromachines 雑誌 11 ( 1 ) 2020

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A lipophilic ionic liquid-based dye for anion optodes: importance of dye lipophilicity and application to heparin measurement

T. Mizuta, S. Takai, T. Nishihata, K. Sueyoshi, T. Endo, H. Hisamoto

Analyst 雑誌 145 5430 - 5437 2020

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Kind of work：Joint Work
Size Sorting of Exosomes by Tuning the Thicknesses of the Electric Double Layers on a Micro-Nanofluidic Device

S. Fujiwara, K. Morikawa, T. Endo, H. Hisamoto, K. Sueyoshi

Micromachines 雑誌 11 ( 5 ) 2020

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Kind of work：Joint Work
Enzyme-responsive Fluorescent Ionic Liquid Reviewed

R. Oishi, T. Mizuta, K. Sueyoshi, T. Endo, H. Hisamoto

Analytical Sciences 雑誌 36 ( 2 ) 143 - 145 2020

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Kind of work：Joint Work
Rapid, simple, sensitive, and multiple enzyme activity assay using a reagent-release capillary-assembled microdevice with double sweeping

Kenji Sueyoshi, Ryota Sanuki, Tatsuro Endo, Hideaki Hisamoto

21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 1291 - 1292 2020

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Publishing type：Research paper (international conference proceedings)

© 17CBMS-0001. In this study, we developed a novel microfluidic platform combining reagent-release capillaries and hydrogels (RRCs and RRGs) for multiple enzyme activity assay. Developed RRC-assembled device provided a rapid, simple and multiple enzyme activity assay by only introducing a sample solution by capillary action. The enzyme reaction products were then concentrated on the basis of double sweeping using both the cationic and anionic micelles from the RRGs by applying voltage. As a result, a rapid, simple, sensitive, and simultaneous assay of caspase-3, trypsin, and alkaline phosphatase was successfully realized by the developed device.
Imprinted photonic crystal nanocavity for p-spectrometer

Kenichi Maeno, Shoma Aki, Kazuo Satoh, Shuichi Murakami, Yusuke Sando, Yusuke Kanaoka, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 561 - 562 2020

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Publishing type：Research paper (international conference proceedings)

© 17CBMS-0001. In this study, novel concept of micrometer-scale spectrometer (P-spectrometer) composed of imprinted photonic crystal nanocavities (PCNs) was proposed. The P-spectrometer has sufficient size to integrate into microfluidic devices and possible to detect light absorption high sensitively. Here we fabricated a PCN as unit cell of P-spectrometer using nanoimprint lithography, and experimentally confirmed for the first time that the fabricated PCN worked as a detection module for 560 nm wavelength.
Design of a PDMS microchannel array-based fast and single-step immunoassay principle using hydrophilic polymer-coated graphene oxide possessing multiple functions

Akihiro Shirai, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 1297 - 1298 2020

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Publishing type：Research paper (international conference proceedings)

© 17CBMS-0001. This paper reports the preparation of the polyethylene glycol-coated graphene oxide (PEG-GO) possessing highly dispersive, fluorescence quenching and size separation functions, and its application to “single-step” immunoassay. The present immunoassay microdevice was fabricated by combining PDMS microchannel arrays separately immobilizing PEG-GO and fluorescently-labelled antibody as soluble coatings. When a sample solution was introduced into the microdevice by capillary action, all the reagents spontaneously mixed, reacted, and free fluorescently-labelled antibody was penetrated into the PEG structure and effectively quenched. Thus the antigen was successfully detected by single-step operation from the change of fluorescence intensity.
Development of a Rapid and Highly Sensitive Plasticized PVC Membrane Optode Utilizing an Ionic Liquid Material Composed of Bromothymol Blue

Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

BUNSEKI KAGAKU 68 ( 12 ) 945 - 951 2019.12（ ISSN:0525-1931 ）

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Publishing type：Research paper (scientific journal)

In this work, ionic liquid materials composed of pH indicator dyes were synthesized and used as a plasticizer for a plasticized PVC membrane-based ion-selective optode. We chose several triphenylmethane dyes and synthesized ionic liquids with those by an ion-exchange procedure, and found that an ionic liquid composed of bromothymol blue (BTB) realized the highest ion-exchange ratio of approximately 100 % (highest concentration of dye). The absorbance response of the PVC membrane using a BTB-based ionic liquid was decreased by ca. 37 % using the repetitive introduction of acid and base solutions, and reached to exhibit a stable and reproducible response. The concentration of dye was 20 similar to 70-times higher than that of the conventional ones. In addition, the PVC membrane was applied for anion sensing based on the coextraction of protons and anions in aqueous solutions, and it was found that the ion selectivity followed the Hofmeister series. In the future, the present ionic liquid material is expected to be applied for highly-selective and sensitive ion-selective optodes by the modification of functional groups such as ion-recognition or reaction with the target molecule.

DOI： 10.2116/bunsekikagaku.68.945

CiNii Article

J-GLOBAL
An ionic liquid composed of purely functional sensing molecules: a colorimetrically calcium responsive ionic liquid Reviewed

Yusuke Niwa, Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

ANALYST 144 ( 23 ) 6858 - 6861 2019.12（ ISSN:0003-2654 ）（ eISSN:1364-5528 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

A highly lipophilic ionic liquid (IL) consisting of stoichiometrically equal amounts of two purely functional chemical sensing molecules, an anionic calcium ionophore and a cationic dye, was synthesized for the first time, and used as a component for a poly (vinyl chloride)-based or neat liquid membrane optical sensor. This is the first report of an IL consisting of purely functional chemical sensing molecules, which is completely different from the previously reported ionic liquids typically consisting of imidazolium or other lipophilic cations. Since the present IL contained an extremely high concentration of dyes, the IL-based sensor showed dramatically enhanced sensitivity (13 times higher compared to that of a conventional sensor), and fully reversible and selective response to Ca2+. Preliminary investigation on the unique response characteristics of the present liquid membrane IL-based sensor was performed with the responses to Ca2+ and Na+.

DOI： 10.1039/c9an01769k

PubMed
TiN-contained polymer-metal core-shell structured nanocone array: Engineering of sensor performance by controlling plasmonic properties Reviewed

Daiki Kawasaki, Kenichi Maeno, Hirotaka Yamada, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

Sensors and Actuators B: Chemical 雑誌 2019.11

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TiN-contained polymer-metal core-shell structured nanocone array: Engineering of sensor performance by controlling plasmonic properties Reviewed

Daiki Kawasaki, Kenichi Maeno, Hirotaka Yamada, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

SENSORS AND ACTUATORS B-CHEMICAL 299 2019.11（ eISSN:0925-4005 ）

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Publishing type：Research paper (scientific journal)

Metal nanostructures have great potential for optical label-free biosensors based on localized surface plasmon resonance (LSPR). The sensitivity of a metal nanostructure-based label-free biosensor (i.e., plasmonic sensor) depends on its plasmonic properties, which suffer a decrease in sensitivity by energy losses in the metal material. Here, we demonstrate an approach to improve the plasmonic properties of metal nanostructures by controlling the carrier density in the base polymer material using titanium nitride (TiN). It is expected that the light energy absorbed by TiN is converted into excitons, and it will assist LSPs in the metal nanostructure; thus, the losses of the metal material are compensated by the excitons excited in TiN. We designed a TiN-contained polymer-metal/core-shell structured nanocone array (NCA), comprising TiN nanoparticles (NPs) in a polymer core and metal shell (Au or Ag), and realized improvement of the refractive index (RI) sensitivity of a label-free biosensor by optimizing the TiN-contained polymer composition. As a result, the TiN-contained polymer-metal NCA, with a TiN NP concentration of 10 wt% in the polymer core, had a 1.5-fold higher RI sensitivity than that of the same NCA without TiN NPs. The results of the resistance measurement of the metal surface with the TiN NP-contained polymer (10 wt%) under light exposure suggest the conversion of exposed light into LSPs of metal via TiN. It is suggested that plasmonic properties and sensor performances can be improved by the presented approach. Moreover, in DNA hybridization detection, an extremely low limit of detection of 117.5 fM was achieved.

DOI： 10.1016/j.snb.2019.126932
TiO2-coated 2D photonic crystals for reflectometric determination of malachite green Reviewed

Bhargav R. Patel, Kenichi Maeno, Hashwin V. S. Ganesh, Tatsuro Endo, Kagan Kerman

MICROCHIMICA ACTA 186 ( 12 ) 844 - 844 2019.11（ ISSN:0026-3672 ）（ eISSN:1436-5073 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

A "detect and destroy" strategy is reported for the spectroscopic determination and photocatalytic degradation of Malachite Green (MG) in aqueous solutions. The intensity of the reflection peak maxima from the TiO2-coated 2D-photonic crystal (PhC) at 633 nm wavelength undergoes a gradual decrease with increasing concentrations of MG. The determination of MG was readily achieved in the nanomolar range due to the quenching of the reflection intensity of the peak, measured using a fiber optic probe. The assay works in the 1.0 nM to 10 mu M MG concentration range with a detection limit of 1.3 nM. The same TiO2-coated 2D-PhC surface can photocatalytically degradeMGin aqueous solutions under UVirradiation. The photocatalytic degradation in the presence of TiO2-coated 2D-PhC becomes evident as the blue color of MG changes to colorless with increasing irradiation time. The decrease in absorption is detected at 617 nm. It was found that the photocatalytic efficiency of TiO2 was synergistically enhanced in the presence of 2D-PhCs. It is concluded that each component of the TiO2-coated 2D-PhC system plays a key role in the detection and degradation of MG.

DOI： 10.1007/s00604-019-3976-6

PubMed
Core-Shell-Structured Gold Nanocone Array for Label-Free DNA Sensing Reviewed

Daiki Kawasaki, Hirotaka Yamada, Kenichi Maeno, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

ACS APPLIED NANO MATERIALS 2 ( 8 ) 4983 - 4990 2019.08（ ISSN:2574-0970 ）

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The refractive index sensitivity of a localized surface plasmon resonance (LSPR) sensor is correlated to an enhanced local electromagnetic (EM) field originating from noble metal nanostructures. Here, we demonstrated that extensive EM field enhancement by a gold (Au) nanocone array (AuNCA) allowed highly sensitive and label-free detection of biomolecules in the visible wavelength spectrum. The AuNCA consisted of a polymer core and an Au shell, which was fabricated by using simple and inexpensive nanoimprint lithography. Under LSPR excitation, AuNCA absorbs visible light of a specific wavelength and extensively enhances the EM field near its surface. It was shown that AuNCA had high refractive index sensitivity (417.5 nm/RIU) because of the large distribution of the enhanced EM field, covering a large surface of the NCA. Moreover, in DNA hybridization detection, a very low limit of detection of 161 fM was achieved, and 1-base mismatch DNA was successfully discriminated by using AuNCA.

DOI： 10.1021/acsanm.9b00930
Core-Shell-Structured Gold Nanocone Array for Label-Free DNA Sensing Reviewed

Daiki Kawasaki, Hirotaka Yamada, Kenichi Maeno, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

ACS Applied Nano Materials 雑誌 2019.07

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Smart Golden Leaves Fabricated by Integrating Au Nanoparticles and Cellulose Nanofibers Reviewed

Hiroshi Shiigi, Tomohiro Tomiyama, Maki Saito, Kengo Ishiki, Dung Q. Nguyen, Tatsuro Endo, Yojiro Yamamoto, Xueling Shan, Zhidong Chen, Tomoaki Nishino, Hidenobu Nakao, Tsutomu Nagaoka

CHEMNANOMAT 5 ( 5 ) 581 - 585 2019.05（ ISSN:2199-692X ）

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A simple processing technology based on spontaneous arrangement of gold nanoparticles (AuNPs) on cellulose nanofibers (CNFs) makes it possible to form a gold leaf with superior mechanical, chemical and electrical characteristics with a small amount of gold (<15vol.%). The electrical resistivity of the AuNP/CNF hybrid film reached 8.7x10(-6)cm at 13vol.% corresponding to that of a gold plate (2.9x10(-6)cm) through a distribution of AuNP inside the film in the form of a band. The AuNPs work not only as linkers between various CNFs in the hybrid structure but also as scaffolds. Self-assembly allows the formation of leaves on three-dimensional micrometer-sized substrates, on which it is impossible to form a uniform flexible metallic layer using conventional techniques such as sputtering, vapor deposition, and electroless plating. The hybrid film holds great promise as a novel gold leaf for a wide range of applications, including coatings, smart papers, electronic skins, and wearable devices owing to its unique combination of superior properties, as well as good productivity, processability, and renewability.

DOI： 10.1002/cnma.201800643
長波長スーパールミネッセントダイオードモジュールの開発 Reviewed

小野　純, 中山貴司, 花藤文希, 杜　暁恩, 小山長規, 遠藤達郎, 森　浩, 山田　誠

電子情報通信学会誌C 雑誌 2019.02

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Smart Golden Leaves Fabricated by Integrating Au Nanoparticles and Cellulose Nanofibers Reviewed

H. Shiigi, T. Tomiyama, M. Saito, K. Ishiki, D. Q. Nguyen, T. Endo, Y. Yamamoto, X. Shan, Z. Chen, T. Nishino, H. Nakao, T. Nagaoka

ChemNanoMat 雑誌 2019.01

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7. Graphene/polyethylene glycol hybrids for single-step immunoassay microdevice Reviewed

Akihiro Shirai, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

FlatChem 2019 ( 13 ) 2019.01
Au "Edged Hole Array" for Sensor Application Reviewed

H. Yamada, D. Kawasaki, K. Sueyoshi, H. Hisamoto, T. Endo

Journal of Photopolymer Science and Technology 雑誌 2019.01

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Graphene/polyethylene glycol hybrids for single-step immunoassay microdevice Reviewed

Akihiro Shirai, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

FLATCHEM 13 34 - 39 2019.01（ ISSN:2452-2627 ）

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In this study, we developed a novel particle-type material comprised of graphene and polyethylene glycol (PEG) to solve the slow response problem of the previously reported microdevice for single-step immunoassay. Characterization of these graphene/PEG hybrids (GPH) was carried out using a transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, and fluorescence analysis. We found that PEG attached to graphene surfaces behave like molecular sieves to separate free fluorescently-labeled antibodies from antigen-bound antibody immunocomplexes. The free antibody is PEG-permeable and directly adsorbs onto the graphene surface, where fluorescence is quenched by fluorescence resonance energy transfer. On the contrary, immunocomplexes are PEG-impermeable; thus, as the concentration of the antigen increases, the concentration of PEG-impermeable immunocomplexes also increases; consequently, increasing the fluorescence intensity of the solution. After characterizing GPH, single-step immunoassay microdevices were prepared by separately immobilizing the GPH and fluorescently-labeled antibodies on two independent microchannel arrays and combining them. Finally, we tested the proposed immunoassay microdevices with various known concentrations of C-reactive protein (CRP), an inflammatory marker protein, and successfully measured CRP concentration within an approximate time of 2 min by monitoring the fluorescence intensity change.

DOI： 10.1016/j.flatc.2018.12.003
ブロモチモールブルーを構成成分とするイオン液体材料に基づく高速・高感度可塑化PVC膜オプトードの開発 Reviewed

水田巽，末吉健志，遠藤達郎，久本秀明

分析化学雑誌 2019

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TiO2-coated 2D photonic crystals for reflectometric determination of malachite green Reviewed

Bhargav R. PatelKenichi MaenoHashwin V. S. GaneshTatsuro EndoKagan Kerman

Microchimica Acta 2019

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Ionic liquid composed of purely functional sensing molecules: Colorimetrically calcium responsive ionic liquid Reviewed

Yusuke Niwa, Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

Analyst 雑誌 2019

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Au "Edged Hole Array" for Sensor Application Reviewed

Hirotaka Yamada, Daiki Kawasaki, Chigusa Inoue, Kenichi Maeno, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

JOURNAL OF PHOTOPOLYMER SCIENCE AND TECHNOLOGY 32 ( 1 ) 101 - 105 2019（ ISSN:0914-9244 ）

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This paper described the design and fabrication of precursor for Au hole structure with four edges (edged hole array: EHA) for sensor application. In this paper, effect of the gap length of opposite edges g and hole side length l was investigated systematically by simulation. As the result, optimal design of EHA that shows strong light absorption (> 90%) and electric field (1.79x10(5)-fold increase against the incident light) at lambda = 785 nm was revealed. Finally, the precursor resist pattern for EHA was fabricated using electron beam lithography and cold development. As the result, the precursor structure that was expected to generate strong electric field at lambda = 785 nm was successfully fabricated.

DOI： 10.2494/photopolymer.32.101
Design and Fabrication of Visible-light-compatible Polymer-based Photonic Crystal Resonator and Waveguide for Sensing Application Reviewed

Jiayi Sun, Kenichi Maeno, Shoma Aki, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

Micromachines 雑誌 2018 ( 9 ) 2018.09

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Development of a polymer/TiO2 hybrid two-dimensional photonic crystal for highly sensitive fluorescence-based ion sensing applications Reviewed

Shoma Aki, Kenichi Maeno, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

Sensors and Actuators B: Chemical 雑誌 269 ( 15 ) 2018.09

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Regioselective immobilization of PVC membrane composed of ionic liquid-based dye on convex-shaped PDMS surface for multiplexed microanalytical devices Reviewed

Tatsumi Mizuta, Kenichi Maeno, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

Analytical Sciences 雑誌 34 ( 5 ) 2018.05

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Photonic crystals on copolymer film for label-free detection of DNA hybridization Reviewed

Han Su, Xin R. Cheng, Tatsuro Endo, Kagan Kerman

Biosensors and Bioelectronics 雑誌 103 2018.04

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Ionic liquid-based dye: A “Dyed plasticizer” for rapid and highly sensitive anion optodes based on a plasticized PVC membrane Reviewed

Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

Sensors and Actuators B: Chemical 雑誌 258 2018.04

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Origin of the Optical Response of a Dye-doped Plasticized Poly(vinyl chloride)-based Photonic Crystal Ion Sensor Reviewed

Shoma Aki, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

Analytical Sciences 雑誌 33 ( 11 ) 2017.11

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Fast and Single-step Fluorescence-based Competitive Bioassay Microdevice Using Combined PDMS Microchannel Arrays Separately Immobilizing Graphene Oxide-Analyte Conjugates and Fluorescently-labelled Receptor Proteins Reviewed

Akihiro Shirai, Kaho Nakashima, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

Analytical Sciences 雑誌 33 ( 8 ) 2017.08

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Double sweeping: Highly effective sample preconcentration using cationic and anionic micelles and its application to a multiple enzyme activity assay Reviewed

Ryota Sanuki, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

Analytical Chemistry 雑誌 89 ( 12 ) 2017.05

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Label-Free Optical Detection of Fibrinogen in Visible Region Using Nanoimprint Lithography-Based Two-Dimensional Photonic Crystal Reviewed

Tatsuro Endo, Hiroshi Kajita

IEICE Transactions on Electronics 雑誌 E100.C ( 2 ) 2017.02

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Development of a single-step immunoassay microdevice based on a graphene oxide-containing hydrogel possessing fluorescence quenching and size separation functions Reviewed

Akihiro Shirai, Kaho Nakashima, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

Analyst 雑誌 142 2017.01

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Fabrication of gold-deposited plasmonic crystal based on nanoimprint lithography for label-free biosensing application Reviewed

Kiichi Nishiguchi, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

Japanese Journal of Applied Physics 雑誌 55 ( 8S3 ) 2016.07

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Development of optical biosensor based on photonic crystal made of TiO2using liquid phase deposition Reviewed

Keigo Aono, Shoma Aki, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

Japanese Journal of Applied Physics 雑誌 55 ( 8S3 ) 2016.07

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Label-free optical detection of C-reactive protein by nanoimprint lithography-based 2D-photonic crystal film Reviewed

Tatsuro Endo, Hiroshi Kajita, Yukio Kawaguchi, Terumasa Kosaka, Toshiyuki Himi

Biotechnology Journal 雑誌 11 ( 6 ) 2016.05

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ナノインプリントリソグラフィを用いたポリマー製二次元フォトニック結晶の作製と化学センサへの応用 Reviewed

遠藤達郎, 梶田浩志

電気学会論文誌Ｅ雑誌 136 ( 4 ) 2016.04

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Fast and single-step immunoassay based on fluorescence quenching within a square glass capillary immobilizing graphene oxide-antibody conjugate and fluorescently labelled antibody Reviewed

Akihiro Shirai, Terence Henares, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

Analyst 雑誌 141 ( 11 ) 2016.04

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A Simple and Rapid Immunoassay Based on Microchip Electrophoresis Using a Reagent-Release Cartridge Reviewed

Kenji Sueyoshi, Yuta Miyahara, Tatsuro Endo, Hideaki Hisamoto

Chromatography 雑誌 37 ( 1 ) 2016.01

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Polymer-based Photonic Crystal Cavity Sensor for Optical Detection in the Visible Wavelength Region Reviewed

Kenichi Maeno, Shoma Aki, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

Analytical Sciences 雑誌 32 ( 1 ) 2016.01

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銀ナノ粒子包含ハイドロゲルと試薬固定化カートリッジを用いた1ステップバイオマーカータンパク質測定デバイスのための要素技術開発 Reviewed

山本健蔵, 末吉健志, 久本秀明, 遠藤達郎

電気学会論文誌Ｃ雑誌 135 ( 11 ) 2015.11

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Highly Sensitive and Multiple Enzyme Activity Assay Using Reagent-release Capillary-Isoelectric Focusing with Rhodamine 110-based Substrates Reviewed

Kenji Sueyoshi, Yuto Nogawa, Kasumi Sugawara, Tatsuro Endo, Hideaki Hisamoto

Analytical Sciences 雑誌 31 ( 11 ) 2015.11

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Fabrication and packaging of a mass-producible capillary-assembled microchip for simple and multiplexed bioassay Reviewed

Terence Henares, Akihiro Shirai, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

Sensors and Actuators B: Chemical 雑誌 215 2015.10

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Development of Microchip Electrophoresis-Integrated Nanoimprinted Photonic Crystal Reviewed

Tatsuro Endo, Kenzo Yamamoto, Kenji Sueyoshi, Hideaki Hisamoto

Sensors and Materials 雑誌 27 ( 5 ) 2015.01

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A single-step enzyme immunoassay capillary sensor composed of functional multilayer coatings for diagnosis marker proteins Reviewed

Shun-ichi Funano, Masato Sugahara, Terence Henares, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

Analyst 雑誌 140 ( 5 ) 2015.01

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Effective Electron Transfer of Self-Assembled Redox-Active Peptide with an Affinity for Glucose Oxidase Reviewed

Shu Taira, Kenji Yokoyama, Daisaku Kaneko, Natsuki Yamaguchi, Tatsuro Endo

Sensors and Materials 雑誌 27 ( 5 ) 2015.01

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Broadband Light Source and Its Application to Near-Infrared Spectroscopy Reviewed

Jun Ono, Tatsuro Endo, Kazuya Ohta, Hirotaka Ono, Yukina Maeda, Kousuke Senda, Osanori Koyama, Makoto Yamada

Sensors and Materials 雑誌 27 ( 5 ) 2015.01

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Plasticized poly(vinyl chloride)-based photonic crystal for ion sensing Reviewed

Shoma Aki, Tatsuro Endo, Kenji Sueyoshi, Hideaki Hisamoto

Analytical Chemistry 雑誌 86 ( 24 ) 2014.11

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Development of novel protease assay device using a nanoimprinted two dimensional photonic crystal Reviewed

Wakana Hashimoto, Tatsuro Endo, Kenji Sueyoshi, Hideaki Hisamoto

Chemistry Letters 雑誌 43 ( 11 ) 2014.08

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Au nanoparticle-modified DNA sensor based on simultaneous electrochemical impedance spectroscopy and localized surface plasmon resonance Reviewed

Xin R. Cheng, Ben Y.H. Hau, Tatsuro Endo, Kagan Kerman

Biosensors and Bioelectronics 雑誌 53 2014.03

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Signal amplified two-dimensional photonic crystal biosensor immobilized with glyco-nanoparticles Reviewed

Yuhei Terada, Wakana Hashimoto, Tatsuro Endo, Hirokazu Seto, Tatsuya Murakami, Hideaki Hisamoto, Yu Hoshino, Yoshiko Miura

Journal of Materials Chemistry B 雑誌 2 ( 21 ) 2014.02

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Efficient Immobilization of Enzyme and Substrate for Single-Step Caspase-3 Inhibitor Assay Using Combinable PDMS Capillary Sensor Array Reviewed

Tadashi Ishimoto, Kaede Jigawa, Terence Henares, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

RSC Advances 雑誌 4 ( 15 ) 2014.01

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Advancements in Capillary-Assembled Microchip (CAs-CHIP) Development for Multiple Analyte Sensing and Microchip Electrophoresis Reviewed

Terence Henares, Shun-ichi Funano, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

Analytical Sciences 雑誌 30 ( 1 ) 2014.01

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電子線描画パターンを用いたプラズモニック結晶の作製とセンサ応用 Reviewed

遠藤達郎, 滝澤光, 栁田保子, 初澤毅

電気学会論文誌Ｅ雑誌 133 ( 12 ) 2013.12

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アルデヒド化合物検出用プラズモニック化学センサの開発 Reviewed

遠藤達郎, 龍野功幸, 栁田保子, 初澤毅

電気学会論文誌Ｅ雑誌 133 ( 12 ) 2013.12

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Capillary-based enzyme-linked immunosorbent assay for highly sensitive detection of thrombin-cleaved osteopontin in plasma Reviewed

Shun-ichi Funano, Terence Henares, Mie Kurata, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

Analytical Biochemistry 雑誌 440 ( 2 ) 2013.09

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Design of Single-Step Immunoassay Principle Based on the Combination of Enzyme-Labeled Antibody Release Coating and Hydrogel Copolymerized with Fluorescent Enzyme Substrate in a Microfluidic Capillary Device Reviewed

Hideki Wakayama, Terence Henares, Kaede Jigawa, Shun-ichi Funano, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

Lab on a Chip 雑誌 13 ( 22 ) 2013.08

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Enhancement of the fluorescence intensity of DNA intercalators using nano-imprinted 2-dimensional photonic crystal Reviewed

Tatsuro Endo, China Ueda, Hiroshi Kajita, Norimichi Okuda, Satoru Tanaka, Hideaki Hisamoto

Microchimica Acta 雑誌 180 ( 9-10 ) 2013.05

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Novel Fluorescent Probe for Highly-Sensitive Bioassay using Sequential Enzyme-Linked Immunosorbent Assay - Capillary Isoelectric Focusing (ELISA-cIEF) Reviewed

Terence Henares, Yuta Uenoyama, Yuto Nogawa, Ken Ikegami, Daniel Citterio, Koji Suzuki, Shun-ichi Funano, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

Analyst 雑誌 138 ( 11 ) 2013.04

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Integration of Neuraminidase Inhibitor Assay into a Single-Step Operation Using Combinable Poly(Dimethylsiloxane) Capillary Sensor Reviewed

Tadashi Ishimoto, Kaede Jigawa, Terence Henares, Tatsuro Endo, Hideaki Hisamoto

Analyst 雑誌 138 ( 11 ) 2013.03

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Photonic crystals on copolymer film for bacteria detection Reviewed

Nan Li, Xin R. Cheng, Arujun Brahmendra, Akriti Prashar, Tatsuro Endo, Cyril Guyard, Mauricio Terebiznik, Kagan Kerman

Biosensors and Bioelectronics 雑誌 41 2013.03

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Open-Type Capillary-Assembled Microchip for Rapid, Single-Step, Simultaneous Multi-Component Analysis of Serum Sample Reviewed

Yusuke Kimura, Terence Henares, Shun-ichi Funano, Tatsuro Endo, Hideaki Hisamoto

RSC Advances 雑誌 2 ( 25 ) 2012.08

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Printed two-dimensional photonic crystals for single-step label-free biosensing of insulin under wet conditions Reviewed

Tatsuro Endo, Masaya Sato, Hiroshi Kajita, Norimichi Okuda, Satoru Tanaka, Hideaki Hisamoto

Lab on a Chip 雑誌 12 ( 11 ) 2012.04

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Photonic Crystal Nanolaser Biosensors Reviewed

Shota Kita, Shota Otsuka, Shoji Hachuda, Tatsuro Endo, Yasunori Imai, Yoshiaki Nishijima, Hiroaki Misawa, Toshihiko Baba

IEICE Transactions on Electronics 雑誌 E95C ( 2 ) 2012.02

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Bulk- and Surface-Modified Combinable PDMS Capillary Sensor Array as Easy-to-Use Sensing Device with Enhanced Sensitivity to Elevated Concentrations of Multiple Serum Sample Components Reviewed

Yuji Fujii, Terence G. Henares, Kunio Kawamura, Tatsuro Endo, Hideaki Hisamoto

Lab on a Chip 雑誌 12 ( 8 ) 2012.01

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Single-Step Sandwich Immunoreaction in a Square Glass Capillary Immobilizing Capture and Enzyme-linked Antibodies for Simplified Enzyme-linked Immunosorbent Assay Reviewed

Erina Tsutsumi, Terence G. Henares, Shun-ichi Funano, Kunio Kawamura, Tatsuro Endo, Hideaki Hisamoto

Analytical Sciences 雑誌 28 ( 1 ) 2012.01

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Direct observation of nodule growth on electroless Ni-P deposition in supercritical CO 2 emulsion Reviewed

Hiroki Uchiyama, Tatsuro Endo, Masato Sone

Journal of The Electrochemical Society 雑誌 159 ( 2 ) 2011.12
プラズモニックナノアレイバイオチップと解析システムの開発 Reviewed

遠藤達郎, 栗田昌昭, 金子努, 齊藤真人, 民谷栄一

電気学会論文誌Ｅ雑誌 131 ( 12 ) 2011.12

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Combinable Poly(Dimethyl siloxane) Capillary Sensor Array for Single-Step and Multiple Enzyme Inhibitor Assays Reviewed

Yosuke Uchiyama, Fumito Okubo, Kozo Akai, Yuji Fujii, Terence G. Henares, Kunio Kawamura, Toshio Yao, Tatsuro Endo, Hideaki Hisamoto

Lab on a Chip 雑誌 12 ( 1 ) 2011.11

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Super-sensitivity in label-free protein sensing using a nanoslot nanolaser Reviewed

Shota Kita, Shoji Hachuda, Shota Otsuka, Tatsuro Endo, Yasunori Imai, Yoshiaki Nishijima, Hiroaki Misawa, Toshihiko Baba

Optics Express 雑誌 19 ( 18 ) 2011.08

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Label-Free Detection of Oligosaccharide-Lectin Interaction Using Plasmonic Optical Device for Glycomics Application Reviewed

Tatsuro Endo, Sayoko Matsuda, Takakiyo Obara, Yasushi Chuma, Makoto Ogata, Yasuko Yanagida, Takeshi Hatsuzawa, Taichi Usui

Sensors and Materials 雑誌 23 ( 2 ) 2011.01

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Study of electrical field distribution of gold-capped nanoparticle for excitation of localized surface plasmon resonance Reviewed

Tatsuro Endo, Hikaru Takizawa, Yasunori Imai, Yasuko Yanagida, Takeshi Hatsuzawa

Applied Surface Science 雑誌 257 ( 7 ) 2011.01

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Localized surface plasmon resonance optical characteristics for hydrogen peroxide using polyvinylpyrrolidone coated silver nanoparticles Reviewed

Tatsuro Endo, Akinobu Shibata, Yasuko Yanagida, Yakichi Higo, Takeshi Hatsuzawa

Materials Letters 雑誌 64 ( 19 ) 2010.10

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プリンタブルフォトニクスによる光学素子作製とバイオセンサヘの応用 Reviewed

遠藤達郎, 奥田覚, 栁田保子，田中覚，初澤毅

電気学会論文誌Ｅ雑誌 130 ( 9 ) 2010.09

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Reflectometric detection of influenza virus in human saliva using nanoimprint lithography-based flexible two-dimensional photonic crystal biosensor Reviewed

Tatsuro Endo, Satoshi Ozawa, Norimichi Okuda, Yasuko Yanagida, Satoru Tanaka, Takeshi Hatsuzawa

Sensors and Actuators B: Chemical 雑誌 148 ( 1 ) 2010.06

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Nanostructured biochip for label-free and real-time optical detection of polymerase chain reaction Reviewed

Ha Minh Hiep, Kagan Kerman, Tatsuro Endo, Masato Saito, Eiichi Tamiya

Analytica Chimica Acta 雑誌 661 ( 2 ) 2010.02

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Fabrication of core-shell structured nanoparticle layer substrate for excitation of localized surface plasmon resonance and its optical response for DNA in aqueous conditions Reviewed

Tatsuro Endo, Daisuke Ikeda, Yukari Kawakami, Yasuko Yanagida, Takeshi Hatsuzawa

Analytica Chimica Acta 雑誌 661 ( 2 ) 2010.02

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Design and fabrication of a dielectrophoresis-based cell-positioning and cell-culture device for construction of cell networks Reviewed

Yasuhiro Tanaka, Tatsuro Endo, Yasuko Yanagidaa, Takeshi Hatsuzawa

Microchemical Journal 雑誌 91 ( 2 ) 2009.03

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Design and fabrication of cell alignment device based on electrolytically-generated air bubbles, and its practical realization using polystyrene microbeads Reviewed

Tatsuro Endo, Kazuhiro Tsuji, Yasuhiro Tanaka, Yasuko Yanagida, Takeshi Hatsuzawa

Microchimica Acta 雑誌 164 ( 3-4 ) 2009.03

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Enhancement of Thermal Properties of Polyvinylpyrrolidone (PVP)-Coated Silver Nanoparticles by Using Plasmid DNA and their Localized Surface Plasmon Resonance (LSPR) Characteristics Reviewed

Tatsuro Endo, Yasuko Yanagida, Takeshi Hatsuzawa

NanoBiotechnology 雑誌 4 ( 1-4 ) 2008.12

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DNA Binding and Bending Protein-Based DNA Actuator and its Practical Realization Reviewed

Tatsuro Endo, Hiroki Yoda, Yasuko Yanagida, Takeshi Hatsuzawa

NanoBiotechnology 雑誌 4 ( 1-4 ) 2008.12

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Quantitative determination of hydrogen peroxide using polymer coated Ag nanoparticles Reviewed

TatsuroEndo, YasukoYanagida, Takeshi Hatsuzawa

Measurement: Journal of the International Measurement Confederation 雑誌 41 ( 9 ) 2008.11

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Evaluation of Cell Adhesion Characteristics on the Porous Silicon Substrates with Various Surface Structures Reviewed

Yasuko Yanagida, Shinsuke Naito, Yasuhiro Tanaka, Tatsuro Endo, Takeshi Hatsuzawa

Electrochemistry 雑誌 76 ( 8 ) 2008.08

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Construction of a biosensor operating on the combined principles of electrochemical analysis and localized surface plasmon resonance for multiple detection of antigen-antibody and enzymatic reactions on the single biosensor Reviewed

Tatsuro Endo, Hikaru Takizawa, Yasuko Yanagida, Takeshi Hatsuzawa, Eiichi Tamiya

Sensors and Materials 雑誌 20 ( 6 ) 2008.06

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Design and fabrication of nanostructures based on DNA ring-protein complex Reviewed

Hideki Furukawa, Tatsuro Endo, Yasuko Yanagida, Takeshi Hatsuzawa

Japanese Journal of Applied Physics 雑誌 47 ( 6 ) 2008.06

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Metallization on polymer by catalyzation in supercritical CO2 and electroless plating in dense CO2 emulsion Reviewed

Byung-Hoon Woo, Masato Sone, Akinobu Shibata, Chiemi Ishiyama, Kaoru Masuda, Masahiro Yamagata, TatsuroEndo, Takeshi Hatsuzawa, Yakichi Higo

Surface and Coatings Technology 雑誌 202 ( 16 ) 2008.05

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環状DNAとタンパク質を用いたナノ構造体の作製－DNA複合構造体作製の基本原理の提案と検証－ Reviewed

古川秀樹, 遠藤達郎, 柳田保子, 初澤毅

精密工学会誌雑誌 74 ( 4 ) 2008.04

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Stimuli-responsive hydrogel-silver nanoparticles composite for development of localized surface plasmon resonance-based optical biosensor Reviewed

TatsuroEndo, Ryuzoh Ikeda, Yasuko Yanagida, Takeshi Hatsuzawa

Analytica Chimica Acta 雑誌 611 ( 2 ) 2008.03

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Label-Free Detection of Melittin Binding to a Membrane Using Electrochemical-Localized Surface Plasmon Resonance Reviewed

Ha Minh Hiep, Tatsuro Endo, Masato Saito, Miyuki Chikae, Do Kyun Kim, Shohei Yamamura, Yuzuru Takamura, Eiichi Tamiya

Analytical Chemistry 雑誌 80 ( 6 ) 2008.02

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Gold nanoparticle-based redox signal enhancement for sensitive detection of human chorionic gonadotropin hormone Reviewed

Koutarou Idegami, Miyuki Chikae, Kagan Kerman, Naoki Nagatani, Teruko Yuhi, Tatsuro Endo, Eiichi Tamiya

Electroanalysis 雑誌 20 ( 1 ) 2008.01

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Enhancement of thermal properties of polymer-coated noble metal nanoparticles using plasmid DNA Reviewed

Tatsuro Endo, Yasuko Yanagida, Takeshi Hatsuzawa

ECS Transactions 雑誌 16 ( 14 ) 2008

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Kind of work：Joint Work
Evaluation of alkyl-chain-length dependency for localized surface plasmon resonance optical characteristics Reviewed

Yukari Kawakami, Tatsuro Endo, Yasuko Yanagida, Takeshi Hatsuzawa, Eiichi Tamiya

ECS Transactions 雑誌 16 ( 14 ) 2008

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Kind of work：Joint Work
Cell cultivation on the porous silicon surface for cell chip application Reviewed

Yasuko Yanagida, Shinsuke Naito, Yasuhiro Tanaka, Tatsuro Endo, Takeshi Hatsuzawa

ECS Transactions 雑誌 16 ( 14 ) 2008

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Kind of work：Joint Work
Rapid and sensitive visual detection of residual pesticides in food using acetylcholinesterase-based disposable membrane chips Reviewed

Naoki Nagatani, Akihiro Takeuchi, M. Anwar Hossain, Teruko Yuhi, Tatsuro Endo, Kagan Kerman, YuzuruTakamura, Eiichi Tamiya

Food Control 雑誌 18 ( 8 ) 2007.08

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Kind of work：Joint Work
Colorimetric detection of volatile organic compounds using a colloidal crystal-based chemical sensor for environmental applications Reviewed

Tatsuro Endo, Yasuko Yanagida, Takeshi Hatsuzawa

Sensors and Actuators B: Chemical 雑誌 125 ( 2 ) 2007.08

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Kind of work：Joint Work
A localized surface plasmon resonance based immunosensor for the detection of casein in milk Reviewed

Ha Mihn Hiep, Tatsuro Endo, Kagan Kerman, Miyuki Chikae, Do-Kyun Kim, Shohei Yamamura, Yuzuru Takamura, Eiichi Tamiya

Science and Technology of Advanced Materials 雑誌 8 ( 4 ) 2007.05

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Kind of work：Joint Work
Excitation of localized surface plasmon resonance using a core-shell structured nanoparticle layer substrate and its application for label-free detection of biomolecular interactions Reviewed

Tatsuro Endo, Kagan Kerman, Naoki Nagatani, Eiichi Tamiya

Journal of Physics Condensed Matter 雑誌 19 ( 21 ) 2007.05

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Kind of work：Joint Work
Label-Free DNA Biosensor Based on Localized Surface Plasmon Resonance Coupled with Interferometry Reviewed

Do-Kyun Kim, Kagan Kerman, Masato Saito, Ramachandra Rao Sathuluri, Tatsuro Endo, Shohei Yamamura, Young-Soo Kwon, Eiichi Tamiya

Analytical Chemistry 雑誌 79 ( 5 ) 2007.03

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Kind of work：Joint Work
Quantum dot-based immunosensor for the detection of prostate-specific antigen using fluorescence microscopy Reviewed

Kagan Kerman, Tatsuro Endo, Masatoshi Tsukamoto, Miyuki Chikae, Yuzuru Takamura, Eiichi Tamiya

Talanta 雑誌 74 ( 4 ) 2007.03

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Kind of work：Joint Work
Uniform Ni – P Film Using an Electroless Plating Method with an Emulsion of Supercritical Carbon Dioxide Reviewed

Hiroki Uchiyama, Masato Sone, Chiemi Ishiyama, Tatsuro Endo, Takeshi Hatsuzawa, Yakichi Higo

Journal of The Electrochemical Society 雑誌 154 ( 7 ) 2007.03

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Kind of work：Joint Work
A sensitive immunochromatographic assay using gold nanoparticles for the semiquantitative detection of prostate-specific antigen in serum Reviewed

Naoki Nagatani, Teruko Yuhi, Miyuki Chikae, Kagan Kerman, Tatsuro Endo, Yoshitomo Kobori, Masayuki Takata, Hiroyuki Konaka, Mikio Namiki, Hiromi Ushijima, Yuzuru Takamura, Eiichi Tamiya

NanoBiotechnology 雑誌 2 ( 3-4 ) 2006.09

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Kind of work：Joint Work
Multiple Label-Free Detection of Antigen−Antibody Reaction Using Localized Surface Plasmon Resonance-Based Core−Shell Structured Nanoparticle Layer Nanochip Reviewed

Tatsuro Endo, Kagan Kerman, Naoki Nagatani, Ha Minh Hiep, Do-Kyun Kim, Yuji Yonezawa, Koichi Nakano, Eiichi Tamiya

Analytical Chemistry 雑誌 78 ( 18 ) 2006.09

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Kind of work：Joint Work
A novel enhancement assay for immunochromatographic test strips using gold nanoparticles Reviewed

Ryo Tanaka, Teruko Yuhi, Naoki Nagatani, Tatsuro Endo, Kagan Kerman, Yuzuru Takamura, Eiichi Tamiya

Analytical and Bioanalytical Chemistry 雑誌 385 ( 8 ) 2006.08

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Kind of work：Joint Work
Resin-based micropipette tip for immunochromatographic assays in urine samples Reviewed

Teruko Yuhi, Naoki Nagatani, Tatsuro Endo, Kagan Kerman, Masayuki Takata, Hiroyuki Konaka, Mikio Namiki, Yuzuru Takamura, Eiichi Tamiya

Journal of Immunological Methods 雑誌 312 ( 1-2 ) 2006.05

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Kind of work：Joint Work
Gold nanoparticle based immunochromatography using a resin modified micropipette tip for rapid and simple detection of human chorionic gonadotropin hormone and prostate-specific antigen Reviewed

Teruko Yuhi, Naoki Nagatani, Tatsuro Endo, Kagan Kerman, Masayuki Takata, Hiroyuki Konaka, Mikio Namiki, Yuzuru Takamura, Eiichi Tamiya

Science and Technology of Advanced Materials 雑誌 7 ( 3 ) 2006.04

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Kind of work：Joint Work
Gold nanoparticle-based novel enhancement method for the development of highly sensitive immunochromatographic test strips Reviewed

Naoki Nagatani, Ryou Tanaka, Teruko Yuhi, Tatsuro Endo, Kagan Kerman, Yuzuru Takamura, Eiichi Tamiya

Science and Technology of Advanced Materials 雑誌 7 ( 3 ) 2006.04

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Kind of work：Joint Work
Label-free detection of peptide nucleic acid-DNA hybridization using localized surface plasmon resonance based optical biosensor Reviewed

Tatsuro Endo, Kagan Kerman, Naoki Nagatani, Yuzuru Takamura, Eiichi Tamiya

Analytical Chemistry 雑誌 77 ( 21 ) 2005.11

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Kind of work：Joint Work
Localized surface plasmon resonance based optical biosensor using surface modified nanoparticle layer for label-free monitoring of antigen-antibody reaction Reviewed

Tatsuro Endo, Shohei Yamamura, Naoki Nagatani, Yasutaka Morita, Yuzuru Takamura, Eiichi Tamiya

Science and Technology of Advanced Materials 雑誌 6 ( 5 ) 2005.07

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Kind of work：Joint Work
Fluorescence-based assay with enzyme amplification on a micro-flow immunosensor chip for monitoring coplanar polychlorinated biphenyls Reviewed

Tatsuro Endo, Akira Okuyama, Yasutaka Matsubara, Kazuto Nishi, Masaaki Kobayashi, Shouhei Yamamura, Yasutaka Morita, Yuzuru Takamura, Haruki Mizukami, Eiichi Tamiya

Analytica Chimica Acta 雑誌 531 ( 1 ) 2005.02

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Kind of work：Joint Work
On-chip micro-flow polystyrene bead-based immunoassay for quantitative detection of tacrolimus (FK506) Reviewed

Yoshihiro Murakami, Tatsuro Endo, Shouhei Yamamura, Naoki Nagatani, Yuzuru Takamura, Eiichi Tamiya

Analytical Biochemistry 雑誌 334 ( 1 ) 2004.11

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Kind of work：Joint Work
Optical-transparent and flexible glucose sensor with ITO electrode Reviewed

Kohji Mitsubayashi, Yoshihiko Wakabayashi, Satoshi Tanimoto, Daisuke Murotomi, Tatsuro Endo

Biosensors and Bioelectronics 雑誌 19 ( 1 ) 2003.10

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Kind of work：Joint Work
A film-type glucose sensor with optical transparency Reviewed

Yoshihiro OYA, Tatsuro ENDOH, Kohji MITSUBAYASHI

Journal of Advanced Science 雑誌 14 ( 1-2 ) 2002

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Kind of work：Joint Work
Study of a film-type oxygen sensor with optical transparency Reviewed

Tatsuro ENDOH, Kohji MITSUBAYASHI

Journal of Advanced Science 雑誌 13 ( 1-2 ) 2001

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Kind of work：Joint Work
Optical-Transparent Oxygen Sensor with a Thinner Membrane Structure Reviewed

Kohji MITSUBAYASHI, Tatsuro ENDO,Satoshi SASAKI, Isao KARUBE

Analytical Sciences 雑誌 17 2001

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Kind of work：Joint Work

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Books and Other Publications

MDB技術予測レポート ─2050年未来・世界を変える技術─

遠藤達郎、安藝翔馬（ Role： Joint author）

株式会社日本能率協会総合研究所 2021.05
暮らしと人を見守る水センシング技術

遠藤達郎（ Role： Joint author）

シーエムシー出版 2019.06

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Responsible for pages：7
IoTを指向するバイオセンシング・デバイス技術

遠藤達郎（ Role： Joint author）

シーエムシー出版 2016.11
ヘルスケアを支えるバイオ計測

遠藤達郎（ Role： Joint author）

シーエムシー出版 2016.03
バイオセンサの先端科学技術と新製品への応用開発

遠藤達郎（ Role： Joint author）

技術情報協会 2014.04
Biochemical Sensors

Tatsuro Endo（ Role： Joint author）

PAN STANFORD PUBLISHING 2013.09
ヘルスケアとバイオ医療のための先端デバイス機器

遠藤達郎（ Role： Joint author）

シーエムシー出版 2009.05
バイオセンサーの先端科学技術と応用

遠藤達郎（ Role： Joint author）

シーエムシー出版 2007.12
動物実験代替のためのバイオマテリアル・デバイス

遠藤達郎（ Role： Joint author）

シーエムシー出版 2007.08
ナノバイオ大辞典

遠藤達郎（ Role： Joint author）

テクノシステム 2007.01
Handbook of Biosensors and Biochips

K. Kerman, T. Endo, E. Tamiya（ Role： Joint author）

Wiley & Sons 2006.10
プラズモンナノ材料の設計と応用技術

遠藤達郎, 民谷栄一（ Role： Joint author）

シーエムシー出版 2006.06

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MISC

Fabrication of Quantum Dots / TiO2 Hybrid Photonic Crystal for Optical Biosensor Application in Visible Region

植田渉太郎, 川崎大輝, 西辻凌輔, 山田大空, 末吉健志, 末吉健志, 久本秀明, 遠藤達郎

応用物理学会春季学術講演会講演予稿集(CD-ROM) 69th 2022（ ISSN:2436-7613 ）

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J-GLOBAL
Development of label-free PCR by using plasmonic crystal

古川ふうた, 山田大空, 川崎大輝, 西辻凌輔, 遠藤達郎, 末吉健志, 末吉健志, 久本秀明

応用物理学会春季学術講演会講演予稿集(CD-ROM) 69th 2022（ ISSN:2436-7613 ）

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J-GLOBAL
Label-free detection of cell metabolites using photonic crystals

WANG Yankang, 中島悠佑, 植田渉太郎, 末吉健志, 末吉健志, 久本秀明, 遠藤達郎

応用物理学会春季学術講演会講演予稿集(CD-ROM) 69th 2022（ ISSN:2436-7613 ）

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J-GLOBAL
ナノ光学素子を用いた非標識バイオセンシング

遠藤達郎

日本生体医工学会大会プログラム・抄録集(Web) 61st 2022

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J-GLOBAL
FRET機構を利用した超高感度カチオンセンシング用蛍光色素液体薄膜の開発

西海豪祐, 水田巽, 末吉健志, 遠藤達郎, 久本秀明

分析化学討論会講演要旨集(Web) 81st 2021

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J-GLOBAL
多段階部分的注入アフィニティキャピラリー電気泳動に基づく核酸-低分子化合物間相互作用解析

光野恵理子, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

分析化学討論会講演要旨集(Web) 81st 2021

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J-GLOBAL
低分子標的アプタマー選抜のためのキャピラリー分子ふるい電気泳動

和田将英, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

分析化学討論会講演要旨集(Web) 81st 2021

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J-GLOBAL
ミクロスケール電気泳動フィルタリングによる高効率アプタマー選抜法の開発

上野楓, 高尾隼空, 飛田安梨沙, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

分析化学討論会講演要旨集(Web) 81st 2021

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J-GLOBAL
フォトニック結晶を用いたDNAメチル化の光検出

中島悠佑, 小林奈緒, 植田渉太郎, 川崎大輝, 末吉健志, 末吉健志, 久本秀明, 遠藤達郎

化学とマイクロ・ナノシステム学会研究会講演要旨集 43rd (CD-ROM) 2021

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J-GLOBAL
Development of a Single-step Bioassay Microdevice Using a Reagent Immobilization Method Based on Inkjet Printing

河相優子, 白井亮洋, 角田正也, 井手上公太郎, 末吉健志, 末吉健志, 遠藤達郎, 久本秀明

分析化学 70 ( 3 ) 125 - 131 2021（ ISSN:0525-1931 ）

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In this work, an inkjet-printed single-step and homogeneous bioassay microdevice was developed with a poly(dimethylsiloxane) (PDMS) microchannel for easy fabrication and mass production. An inkjet printer can dispense reactive reagents as nanoliter droplets on a PDMS microchannel accurately. Therefore, homogeneous and reproducible dispensing of reagents can be achieved. In a previous report, a complicated fabrication procedure for combining two PDMS microchannel arrays immobilizing two reactive reagents was required. Therefore, in the present report, a new immobilizing method, which involves the patterning of two reactive reagents on two bottom corners of a PDMS microchannel as reagent spots separately based on inkjet printing, was developed. Based on this method, we developed single-step and homogeneous bioassay microdevice that patterns a biotin-sulfonic acid group-introduced graphene oxide conjugate and a fluorescently labeled streptavidin with the same microchannel. For a homogeneous bioassay, the dissolution of immobilized reactive reagents by sample introduction is essential. Therefore, the immobilization matrix, which was mixed with reactive reagents, was investigated. We found that trehalose exhibited favorable characteristics concerning rapid dissolution and uniformity. Then, a single-step detection of biotin was carried out. The detection limit was 0.68 mL(-1), ng which was approximately 600-times lower than that of previously reported method. Furthermore, biotin selectivity based on a bioassay microdevice was confirmed. In the future, an immobilization method based on inkjet printing can be applied to various bioassay devices based on the microchannel.

DOI： 10.2116/bunsekikagaku.70.125

J-GLOBAL
高精度な細胞外微粒子分級用マイクロ・ナノ流体デバイスの開発

藤原聡子, 金子紗也, 森川響二朗, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

日本分析化学会年会講演要旨集(Web) 69th 2020

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J-GLOBAL
高感度イオンセンシングを志向した色素液体ナノエマルションの創製

牧佳穂, 大石綾太郎, 水田巽, 末吉健志, 遠藤達郎, 久本秀明

日本分析化学会年会講演要旨集(Web) 69th 2020

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J-GLOBAL
Evaluation of surface treatment conditions for reduction of non-specific adsorption on polymer-based photonic crystal

中野萌, 山田大空, 川崎大輝, 末吉健志, 久本秀明, 遠藤達郎

日本化学会春季年会講演予稿集(CD-ROM) 100th 2020

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J-GLOBAL
電気泳動フィルタリングによる標的分子捕捉とDNA結合・解離を利用した新規アプタマー選抜法の開発

高尾隼空, 飛田安梨沙, 上野楓, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

キャピラリー電気泳動シンポジウム講演要旨集 40th (CD-ROM) 2020

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J-GLOBAL
蛍光性色素液体を用いた超高感度FRET検出型PVC膜オプトードの開発

水田巽, 末吉健志, 遠藤達郎, 久本秀明

分析化学討論会講演要旨集(Web) 80th 2020

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J-GLOBAL
蛍光性色素液体を用いたFRET検出型PVC膜オプトードの開発と超高感度イオンセンシングへの応用

水田巽, 末吉健志, 遠藤達郎, 久本秀明

日本分析化学会年会講演要旨集(Web) 69th 2020

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J-GLOBAL
色素液体ナノエマルションの創製と高感度イオンセンシングの基礎検討

牧佳穂, 大石綾太郎, 水田巽, 末吉健志, 遠藤達郎, 久本秀明

分析化学討論会講演要旨集(Web) 80th 2020

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J-GLOBAL
液状イオノフォアと固体色素に基づく高感度アンモニウムイオンセンシング液体開発の基礎検討

岡嵩人, 水田巽, 末吉健志, 末吉健志, 遠藤達郎, 遠藤達郎, 久本秀明

分析化学討論会講演要旨集(Web) 80th 2020

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J-GLOBAL
核酸アプタマー高効率選抜のためのミクロスケール電気泳動フィルタリングデバイス

末吉健志, 末吉健志, 永井伶奈, 遠藤達郎, 久本秀明

日本分析化学会年会講演要旨集(Web) 69th 2020

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J-GLOBAL
多段階部分的注入キャピラリー電気泳動法による核酸-低分子化合物間相互作用解析

光野恵理子, 遠藤達郎, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

分析化学討論会講演要旨集(Web) 80th 2020

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J-GLOBAL
多段階部分的注入アフィニティーキャピラリー電気泳動法による疾患関連核酸-低分子化合物間相互作用解析

光野恵理子, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

キャピラリー電気泳動シンポジウム講演要旨集 40th (CD-ROM) 2020

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J-GLOBAL
多段階部分的注入アフィニティーキャピラリー電気泳動法による物質間相互作用解析

光野恵理子, 久本秀明, 遠藤達郎, 末吉健志, 末吉健志

日本分析化学会年会講演要旨集(Web) 69th 2020

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J-GLOBAL
Fabrication and characterization of YAG:Ce3+ phosphor powder contained polymer-based photonic crystal for optical sensor application

植田渉太郎, 小林奈緒, 川崎大輝, 山田大空, 末吉健志, 末吉健志, 久本秀明, 遠藤達郎, 遠藤達郎

応用物理学会春季学術講演会講演予稿集(CD-ROM) 67th 2020

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J-GLOBAL
Fabrication of Plasmonic Membrane for Sensor Application using Membrane Filter as a Template

遠藤達郎, 山田大空

電気学会論文誌 E 140 ( 12 ) 2020（ ISSN:1341-8939 ）

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J-GLOBAL
ミクロスケール電気泳動フィルタリングに基づく核酸アプタマー高効率選抜デバイスの開発

飛田安梨沙, 高尾隼空, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

化学とマイクロ・ナノシステム学会研究会講演要旨集 42nd (Web) 2020

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J-GLOBAL
ミクロスケール電気泳動フィルタリングによるアプタマー選抜法の開発

飛田安梨沙, 上野楓, 高尾隼空, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

キャピラリー電気泳動シンポジウム講演要旨集 40th (CD-ROM) 2020

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J-GLOBAL
Label-free monitoring of cell stimulation kinetics using polymer-based photonic crystal

遠藤達郎, 柳田保子

生体医歯工学共同研究拠点成果報告会 2019 2020

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J-GLOBAL
プラズモニック結晶を用いた非染色PCR産物検出デバイスの開発

古川ふう大, 井上千種, 川崎大輝, 山田大空, 末吉健志, 末吉健志, 久本秀明, 遠藤達郎

日本分析化学会年会講演要旨集(Web) 69th 2020

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J-GLOBAL
Development of cell analysis platform based on nanophotonics

遠藤達郎, 柳田保子

生体医歯工学共同研究拠点成果報告会 2019 2020

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J-GLOBAL
Development of Bio Analytical Devices Based on Nanoimprint Lithography

ENDO Tatsuro

Journal of the Japan Society for Precision Engineering 86 ( 4 ) 251 - 254 2020（ ISSN:0912-0289 ）

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DOI： 10.2493/jjspe.86.251

CiNii Article
キャピラリー分子ふるい電気泳動を利用した低分子標的アプタマー選抜法

和田将英, 遠藤達郎, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

分析化学討論会講演要旨集(Web) 80th 2020

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J-GLOBAL
キャピラリー分子ふるい電気泳動に基づく低分子標的アプタマー選抜法

和田将英, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

日本分析化学会年会講演要旨集(Web) 69th 2020

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J-GLOBAL
キャピラリー分子ふるい電気泳動による低分子標的核酸アプタマー選抜法

和田将英, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

キャピラリー電気泳動シンポジウム講演要旨集 40th (CD-ROM) 2020

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J-GLOBAL
電気泳動フィルタリングによる酵素濃縮・生成物分離を利用した酵素反応初速度測定

高尾隼空, 遠藤達郎, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

キャピラリー電気泳動シンポジウム講演要旨集 39th 2019

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J-GLOBAL
部分的注入アフィニティキャピラリー電気泳動に基づく核酸標的薬剤スクリーニング

末吉健志, 末吉健志, 光野恵理子, 西野智哉, 遠藤達郎, 遠藤達郎, 久本秀明

キャピラリー電気泳動シンポジウム講演要旨集 39th 2019

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J-GLOBAL
ナノ流路内電気二重層厚さ制御に基づくエクソソーム分級

藤原聡子, 森川響二朗, 遠藤達郎, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

キャピラリー電気泳動シンポジウム講演要旨集 39th 2019

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J-GLOBAL
キャピラリー分子ふるい電気泳動による低分子認識アプタマーの高効率選抜法

和田将英, 遠藤達郎, 遠藤達郎, 久本秀明, 末吉健志, 末吉健志

キャピラリー電気泳動シンポジウム講演要旨集 39th 2019

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Inkjet Printingを用いた試薬固定に基づく1stepビオチン測定マイクロデバイスの開発

河相優子, 角田正也, 末吉健志, 遠藤達郎, 久本秀明

化学とマイクロ・ナノシステム学会研究会講演要旨集 39th 2019

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J-GLOBAL
Inkjet Printingを用いた1step競合型イムノアッセイマイクロデバイスの開発

河相優子, 角田正也, 末吉健志, 遠藤達郎, 久本秀明

日本分析化学会年会講演要旨集(Web) 68th 2019

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Presentations

可塑化PVC被覆フォトニック結晶イオンセンサの開発 Domestic conference

山崎友暉、末吉健志、久本秀明、遠藤達郎

2024年第71回応用物理学会春季学術講演会 2024.03

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Presentation type：Oral presentation (general)
疾病の早期診断を志向したナノインプリンテッド光学センサの開発 Invited Domestic conference

遠藤達郎

センシング技術応用研究会　第226回研究例会 2024.03 センシング技術応用研究会

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Presentation type：Public lecture, seminar, tutorial, course, or other speech
マイクロチップ電気泳動フィルタリングデバイスを用いたアプタマー選抜 Domestic conference

河野歩夢、遠藤達郎、久本秀明、末吉健志

第43回キャピラリー電気泳動シンポジウム 2023.11

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Presentation type：Poster presentation
アプタマー選抜用PMMA製マイクロチップ電気泳動フィルタリングデバイスの作製・評価 Domestic conference

河野歩夢、遠藤達郎、久本秀明、末吉健志

第43回キャピラリー電気泳動シンポジウム 2023.11
マイクロチップ電気泳動フィルタリングデバイスを用いたアプタマー選抜の検討 Domestic conference

河野歩夢、遠藤達郎、久本秀明、末吉健志

化学とマイクロ・ナノシステム学会第48回研究会 2023.11
エクソソームの粒子径選択的分離用マイクロ・ナノスケール電気泳動デバイスの開発 Domestic conference

阿部友哉、森川響二朗、遠藤達郎、久本秀明、末吉健志

第10回日本細胞外小胞学会学術集会 2023.10
Ionic liquid-based dye nanoemulsion using merocyanine dye: highly-sensitive and rapidly-responsive ion-sensing component of hydrogel-based microanalytical devices International conference

Yu Koizumi, Shuto Oka, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

The 27th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2023) 2023.10

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Presentation type：Poster presentation
Selection of aptamers binding small molecules by capillary sieving electrophoresis International conference

Masahide Wada, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi

大阪公立大学大学院工学研究科国際シンポジウム～世界をリードする工学研究～ 2023.09

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Presentation type：Poster presentation
Biomolecules detection in visible region with gold nanohole arrays via dielectrophoresis International conference

Satoko Fujiwara, Daiki Kawasaki, Kenji Sueyoshi, Hideaki Hisamoto, Masato Suzuki, Tomoyuki Yasukawa, Tatsuro Endo

大阪公立大学大学院工学研究科国際シンポジウム～世界をリードする工学研究～ 2023.09
Fluorescence optical sensors for water detection based on intramolecular charge transfer-type dyes International conference

Ami Morimoto, Naoya Suzuki, Shigeyuki Yagi, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

大阪公立大学大学院工学研究科国際シンポジウム～世界をリードする工学研究～ 2023.09

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Presentation type：Poster presentation
次元削減手法を用いたSERSスペクトルの解析 Domestic conference

西辻凌輔、中島智晴、末吉健志、久本秀明、遠藤達郎

第84回応用物理学会秋季学術講演会 2023.09
基質部位・発光部位を独立させた新規酵素応答性ナノエマルションセンサー開発の基礎検討 Domestic conference

和田奈由子、末吉健志、遠藤達郎、久本秀明

日本分析化学会第72年会 2023.09
フッ化アルキル含有ナノエマルション型オプトードに基づく高選択的PFOSセンシングの検討 Domestic conference

岩本空果、末吉健志、遠藤達郎、久本秀明

日本分析化学会第72年会 2023.09

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Presentation type：Poster presentation
アプタマー選抜⽤マイクロチップ電気泳動フィルタリングデバイスの開発 Domestic conference

河野歩夢、遠藤達郎、久本秀明、末吉健志

日本分析化学会第72年会 2023.09

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Presentation type：Poster presentation
デジタル分⼦ふるい電気泳動⽤キャピラリーデバイスの開発 Domestic conference

末吉健志、松田景太、遠藤達郎、久本秀明

日本分析化学会第72年会 2023.09

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Presentation type：Oral presentation (general)
イオンセンサー用ナノエマルション内での色素-イオノフォア相互作用の検討 Domestic conference

松本尚也、末吉健志、遠藤達郎、久本秀明

日本分析化学会第72年会 2023.09
Selection of aptamers binding small molecules by capillary sieving electrophoresis International conference

Masahide Wada, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi

RSC Tokyo International Conference 2023 2023.09

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Presentation type：Poster presentation
Detection of biomolecules in visible region with gold nanohole arrays via dielectrophoresis for biosensor application International conference

Satoko Fujiwara, Daiki Kawasaki, Kenji Sueyoshi, Hideaki Hisamoto, Masato Suzuki, Tomoyuki Yasukawa, Tatsuro Endo

RSC Tokyo International Conference 2023 2023.09

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Presentation type：Poster presentation
Label-free detection of cell metabolites using nanoimprint lithography-based photonic crystals International conference

Yankang Wang, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

The 9th TKU-ECUST-KIST-OMU-UH-IHU-KMITL-UTAR-TNU-HUFI Joint Symposium onAdvanced Materials and Applications (JSAMA-9) 2023.09

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Presentation type：Poster presentation
可塑化PVC被覆型フォトニック結晶の開発とイオンセンシングへの応用 Domestic conference

山崎友暉、末吉健志、久本秀明、遠藤達郎

第17回近畿支部夏季セミナー～ぶんせき秘帖巻ノ拾七～ 2023.08

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Presentation type：Poster presentation
pH非依存性ナノエマルション型イオンセンサーのイオノフォア構造と応答挙動の検討 Domestic conference

松本尚也、末吉健志、遠藤達郎、久本秀明

第17回近畿支部夏季セミナー～ぶんせき秘帖巻ノ拾七～ 2023.08
アプタマー選抜用マイクロチップ電気泳動フィルタリングデバイスの開発 Domestic conference

河野歩夢、遠藤達郎、久本秀明、末吉健志

第17回近畿支部夏季セミナー～ぶんせき秘帖巻ノ拾七～ 2023.08

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フッ化アルキル含有ナノエマルション型オプトードに基づくPFOSセンシングの検討 Domestic conference

岩本空果、末吉健志、遠藤達郎、久本秀明

第17回近畿支部夏季セミナー～ぶんせき秘帖巻ノ拾七～ 2023.08

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マイクロ流体デバイスによる色素液体ナノエマルション作製方法の検討 Domestic conference

安田悠一郎、末吉健志、遠藤達郎、真栄城正寿、渡慶次学、久本秀明

第17回近畿支部夏季セミナー～ぶんせき秘帖巻ノ拾七～ 2023.08

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マイクロ・ナノ流路内電気二重層厚さ制御に基づくエクソソーム分級法の開発 Domestic conference

阿部友哉、藤原聡子、森川響二朗、遠藤達郎、久本秀明、末吉健志

日本分析科学会近畿支部創設70周年記念式典 2023.06

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ナノエマルション型オプトードに基づくPFOSセンシングの検討 Domestic conference

岩本空果、末吉健志、遠藤達郎、久本秀明

第83回分析化学討論会 2023.05

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キャピラリー分子ふるい電気泳動に基づく低分子標的-構造誘起型アプタマー選抜 Domestic conference

和田将英、遠藤達郎、久本秀明、末吉健志

第83回分析化学討論会 2023.05

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マイクロ流体デバイスによる色素液体ナノエマルション作製方法の検討 Domestic conference

安田悠一郎、末吉健志、遠藤達郎、真栄城正寿、渡慶次学、久本秀明

第83回分析化学討論会 2023.05

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分子内水素結合をもつICT型色素を利用した有機溶媒中水分センシング膜の設計と開発 Domestic conference

森本あみ、末吉健志、遠藤達郎、久本秀明

第83回分析化学討論会 2023.05

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Presentation type：Poster presentation
pH非依存ナノエマルション型イオンセンサーのイオノフォア構造と応答挙動の検討 Domestic conference

松本尚也、末吉健志、遠藤達郎、久本秀明

第83回分析化学討論会 2023.05

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Presentation type：Poster presentation
ポリマーフォトニック結晶およびプラズモニック結晶の近接場光学応答評価 Domestic conference

延本美優、川﨑大輝、末吉健志、久本秀明、遠藤達郎

第83回分析化学討論会 2023.05

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電気泳動フィルタリングに基づくアプタマー選抜法の開発とその応用 Domestic conference

末吉健志、高尾隼空、上野楓、遠藤達郎、久本秀明

第83回分析化学討論会 2023.05

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ナノフォトニクスを基盤技術とした生体情報計測 Domestic conference

遠藤達郎

第62回日本生体医工学会大会 2023.05 日本生体医工学会

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Presentation type：Oral presentation (general)

Venue：名古屋国際会議場
Discrimination of self-assembled monolayer composition on gold nanostructure surface by combining machine learning and surface enhanced Raman scattering International conference

Ryosuke Nishitsuji, Tomoharu Nakashima, Kenji Sueyoshi, Hideaki Hisamoto, Tatsuro Endo

SPIE Future Sensing Technologies 2023 2023.04

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Presentation type：Poster presentation
蛍光性A–π–D型色素の設計・合成と有機溶媒中水分センシング膜の作製 Domestic conference

森本あみ，末吉健志，遠藤達郎，久本秀明

日本化学会第103春季年会 2023.03

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Venue：東京理科大学
ナノインプリント製フォトニック結晶を用いたメチル化シトシン検出 Domestic conference

中島悠佑，川崎大輝，末吉健志，久本秀明，遠藤達郎

第70回応用物理学会春季学術講演会 2023.03

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Venue：上智大学
イオン選択性色素液体ナノエマルション含有ゲルの開発と1ステップ分析デバイスへの応用 Domestic conference

小泉有右，末吉健志，遠藤達郎，久本秀明

化学とマイクロ・ナノシステム学会第46回研究会 2022.11
フォトニック結晶を用いたDNAメチル化解析 Domestic conference

中島悠佑，川﨑大輝，末吉健志，久本秀明，遠藤達郎

化学とマイクロ・ナノシステム学会第46回研究会 2022.11

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Venue：アスティとくしま
ミクロスケール電気泳動フィルタリングデバイスを用いた高効率アプタマー選抜 Domestic conference

高尾隼空，上野楓，飛田安梨沙，遠藤達郎，久本秀明，末吉健志

化学とマイクロ・ナノシステム学会第46回研究会 2022.11

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Venue：アスティとくしま
ミクロスケール電気泳動フィルタリングによる異種標的分子混合試料に対するアプタマー同時選抜 Domestic conference

上野楓，遠藤達郎，久本秀明，末吉健志

第42回キャピラリー電気泳動シンポジウム 2022.10

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Venue：鶴岡市先端研究産業支援センター
インクジェットプリンティングによる試薬固定化法を利用する1ステップバイオアッセイマイクロデバイスの開発 Domestic conference

河相優子，白井亮洋，角田正也，井手上公太郎，末吉健志，遠藤達郎，久本秀明

日本分析化学会第71年会 2022.09

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Venue：岡山大学
キャピラリー分子ふるい電気泳動法による低分子標的・構造誘起型アプタマー選抜 Domestic conference

和田将英，遠藤達郎，久本秀明，末吉健志

日本分析化学会第71年会 2022.09

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Venue：岡山大学
電気泳動フィルタリングデバイスを用いたエクソソームアプタマー選抜・解析 Domestic conference

末吉健志，飛田安梨沙，上野楓，高尾隼空，遠藤達郎，久本秀明

日本分析化学会第71年会 2022.09

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Venue：岡山大学
蛍光性膜溶媒 / 蛍光色素間のFRET機構を利用した高感度カチオンセンシング用蛍光色素液体薄膜の開発 Domestic conference

西海豪祐，水田巽，末吉健志，遠藤達郎，久本秀明

日本分析化学会第71年会 2022.09

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Venue：岡山大学
ミクロスケール電気泳動フィルタリングに基づく簡便なアプタマー選抜 Domestic conference

高尾隼空，遠藤達郎，久本秀明，末吉健志

日本分析化学会第71年会 2022.09

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Venue：岡山大学
キャピラリー分子ふるい電気泳動による低分子標的構造誘起型アプタマー選抜 Domestic conference

和田将英，遠藤達郎，久本秀明，末吉健志

第29回クロマトグラフィーシンポジウム 2022.06

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Venue：沖縄県石垣市
マイクロ・ナノ流路内電気二重層厚さ調節に基づくナノ粒子分級用デバイスの開発 Domestic conference

阿部友哉，藤原聡子，森川響二朗，遠藤達郎，久本秀明，末吉健志

化学とマイクロ・ナノシステム学会第45回研究会 2022.05
広範囲増強電場を有するプラズモニックナノコーンアレイの作製とSERSセンサへの展開 Domestic conference

藤原聡子，川﨑大輝，末吉健志，久本秀明，遠藤達郎

化学とマイクロ・ナノシステム学会第45回研究会 2022.05

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Venue：中央大学
TiO2フォトニック結晶を用いたエンドトキシン検出法の開発 Domestic conference

西辻凌輔，植田渉太郎，末吉健志，久本秀明，遠藤達郎

化学とマイクロ・ナノシステム学会第45回研究会 2022.05

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Venue：中央大学
ミクロスケール電気泳動フィルタリングデバイスを用いた1ラウンドアプタマー選抜 Domestic conference

末吉健志，高尾隼空，飛田安梨沙，上野楓，遠藤達郎，久本秀明

化学とマイクロ・ナノシステム学会第45回研究会 2022.05

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Venue：中央大学
プラズモニック/フォトニック複合構造体による高感度DNA解析 Domestic conference

川﨑大輝，末吉健志，久本秀明，遠藤達郎

化学とマイクロ・ナノシステム学会第45回研究会 2022.05
酵素基質・色素液体に基づく酵素応答性オプトードの基礎検討 Domestic conference

和田奈由子，末吉健志，遠藤達郎，久本秀明

日本分析化学会第82回分析化学討論会 2022.05

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Venue：茨城大学
アニオン選択性色素液体ナノエマルションの開発：エマルション内色素－イオノフォア間相互作用による応答性能向上 Domestic conference

末吉健志，和田将英，遠藤達郎，久本秀明

日本分析化学会第82回分析化学討論会 2022.05

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Venue：茨城大学
核酸アプタマー選抜におけるキャピラリー分子ふるい電気泳動に基づく構造特性分離

牧佳穂，大石綾太郎，水田巽，末吉健志，遠藤達郎，久本秀明

日本分析化学会第82回分析化学討論会 2022.05

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Venue：茨城大学
Quantum Dots / TiO2 Hybrid Photonic Crystal: Fabrication and Characterization for Optical Biosensor Application in Visible Region International conference

Shotaro Ueda, Daiki Kawasaki, Hirotaka Yamada, Ryosuke Nishitsuji, Kenji Sueyoshi, Hideaki Hisamoto, and Tatsuro Endo

Royal Society of Chemistry Tokyo International Conference 2021 (RSC-TIC2021) 2021.12

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Presentation type：Poster presentation
Highly-sensitive cation sensing optodes based on FRET-enhanced fluorescence using fluorescent dyed plasticizer International conference

Kosuke Nishiumi, Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

Royal Society of Chemistry Tokyo International Conference 2021 (RSC-TIC2021) 2021.12

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Presentation type：Poster presentation
Development of highly sensitive and selective plasticized PVC membrane optical sensor based on silver-responsive ionic liquid based-dye International conference

Shuto Oka, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

Royal Society of Chemistry Tokyo International Conference 2021 (RSC-TIC2021) 2021.12

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Presentation type：Poster presentation
キャピラリー分子ふるい電気泳動によるランダム配列DNAの構造安定性に基づく分離 Domestic conference

和田将英，遠藤達郎，久本秀明，末吉健志

第41回キャピラリー電気泳動シンポジウム 2021.12

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部分的注入アフィニティキャピラリー電気泳動による四重鎖核酸－低分子間相互作用解析 Domestic conference

光野恵理子，遠藤達郎，久本秀明，末吉健志

第41回キャピラリー電気泳動シンポジウム 2021.12

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ミクロスケール電気泳動フィルタリングによるエクソソームアプタマー選抜 Domestic conference

飛田安梨沙，上野楓，高尾隼空，遠藤達郎，久本秀明，末吉健志

第41回キャピラリー電気泳動シンポジウム 2021.12

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Presentation type：Poster presentation
ナノインプリント技術を用いた光学素子の作製とバイオセンサ応用 Invited Domestic conference

遠藤達郎

電子情報通信学会POC研究会 2021.11

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Presentation type：Oral presentation (invited, special)
ポリマーを基材としたナノバイオセンシングデバイスの創製 Invited Domestic conference

遠藤達郎

第30回ポリマー材料フォーラム 2021.11

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キャピラリー分子ふるい電気泳動に基づく低分子標的構造誘起型アプタマー選抜法 Domestic conference

和田将英，遠藤達郎，久本秀明，末吉健志

第32回クロマトグラフィー科学会議 2021.11

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Presentation type：Poster presentation
ヒドロゲル部分充填キャピラリーデバイスを用いた高効率アプタマー電気泳動選抜法の開発 Domestic conference

末吉健志，高尾隼空，飛田安梨沙，上野楓，遠藤達郎，久本秀明

第32回クロマトグラフィー科学会議 2021.11

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Presentation type：Poster presentation
高感度イオン検出を志向した色素液体ナノエマルションの開発とマイクロ分析デバイスへの応用 Domestic conference

牧佳穂，末吉健志，遠藤達郎，久本秀明

化学とマイクロ・ナノシステム学会第44回研究会 2021.11

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ミクロスケール電気泳動フィルタリングデバイスを用いた核酸アプタマー選抜 Domestic conference

高尾隼空，飛田安梨沙，上野楓，遠藤達郎，久本秀明，末吉健志

化学とマイクロ・ナノシステム学会第44回研究会 2021.11

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ポリマー製フォトニック結晶シートを用いたスマートフォンによるSERS-CoV-2スパイクタンパク質の高感度検出 Domestic conference

川崎大輝，山田大空，末吉健志，久本秀明，遠藤達郎

化学とマイクロ・ナノシステム学会第44回研究会 2021.11

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イオン選択性可塑化PVC複合型プラズモニックイオンセンサの開発 Domestic conference

遠藤達郎，井上千種，川崎大輝，山田大空，末吉健志，久本秀明

第38回「センサ・マイクロマシンと応用システム」シンポジウム 2021.11

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IONIC LIQUID-BASED DYE (IL-DYE) NANOEMULSION (NE) AS A HIGH-SENSITIVITY ION SENSING COMPONENT OF MICRO ANALYTICAL DEVICES International conference

Kaho Maki, Kenji Sueyoshi, Tatsuro Endo, and Hideaki Hisamoto

The 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021) 2021.10

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ミクロスケール電気泳動フィルタリングに基づくアプタマー選抜法の基礎評価・高効率化 Domestic conference

上野楓，高尾隼空，飛田安梨沙，遠藤達郎，久本秀明，末吉健志

第11回CSJ化学フェスタ2021 2021.10

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固定化不要な標的分子捕捉および高効率なDNA結合を利用したアプタマーの電気泳動選抜法 Domestic conference

高尾隼空，飛田安梨沙，上野楓，久本秀明，遠藤達郎，末吉健志

日本分析化学会第70年会 2021.09

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キャピラリー分子ふるい電気泳動に基づく低分子標的構造誘起型アプタマー選抜法 Domestic conference

末吉健志，和田将英，遠藤達郎，久本秀明

日本分析化学会第70年会 2021.09

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FRET型蛍光色素液体ナノエマルションの応答機構の基礎検討 Domestic conference

小泉有右，水田巽，末吉健志，遠藤達郎，久本秀明

日本分析化学会第70年会 2021.09

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銀イオン応答性色素液体の開発と高感度・高選択的オプティカルセンサーへの応用 Domestic conference

岡嵩人，末吉健志，遠藤達郎，久本秀明

日本分析化学会第70年会 2021.09

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酵素含有刺激応答性ハイドロゲル/フォトニック結晶ハイブリッドの作製とグルコースセンサへの応用 Domestic conference

遠藤達郎, 小林奈緒, 川崎大輝, 山田大空, 末吉健志, 久本秀明

第60回日本生体医工学会大会・第36回日本生体磁気学会大会 2021.06

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バイオセンシング応用を指向したプラズモニックデバイスの開発 Invited Domestic conference

遠藤達郎

第20回プラズモニック化学シンポジウム 2021.06

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アプタマー選抜のための安定構造形成型・非形成型DNAライブラリーの調製 Domestic conference

和田将英，遠藤達郎，久本秀明，末吉健志

日本核酸医薬学会第6回年会 2021.06

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ミクロスケール電気泳動フィルタリングによるアプタマー選抜法の開発 Domestic conference

飛田安梨沙，上野楓，高尾隼空，遠藤達郎，久本秀明，末吉健志

日本核酸医薬学会第6回年会 2021.06

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キャピラリー分子ふるい電気泳動に基づく低分子標的アプタマー選抜のための構造形成型／非形成型核酸ライブラリの構築 Domestic conference

末吉健志，和田将英，遠藤達郎，久本秀明

第 28 回クロマトグラフィーシンポジウム 2021.06

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フォトニック結晶を用いたDNAメチル化の光検出 Domestic conference

中島悠佑，小林奈緒，植田渉太郎，川崎大輝，末吉健志，久本秀明，遠藤達郎

化学とマイクロ・ナノシステム学会第43回研究会 2021.05

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低分子標的アプタマー選抜のためのキャピラリー分子ふるい電気泳動 Domestic conference

和田将英，遠藤達郎，久本秀明，末吉健志

日本分析化学会第81回分析化学討論会 2021.05

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多段階部分的注入アフィニティキャピラリー電気泳動に基づく核酸-低分子化合物間相互作用解析 Domestic conference

光野恵理子，遠藤達郎，久本秀明，末吉健志

日本分析化学会第81回分析化学討論会 2021.05

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FRET機構を利用した超高感度カチオンセンシング用蛍光色素液体薄膜の開発 Domestic conference

西海豪祐，水田巽，末吉健志，遠藤達郎，久本秀明

日本分析化学会第81回分析化学討論会 2021.05

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ミクロスケール電気泳動フィルタリングによる高効率アプタマー選抜法の開発 Domestic conference

上野楓，高尾隼空，飛田安梨沙，遠藤達郎，久本秀明，末吉健志

日本分析化学会第81回分析化学討論会 2021.05

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細胞機能解析プラットフォーム構築に向けたナノデバイスの開発

遠藤達郎, 柳田保子

生体医歯工学共同研究拠点成果報告書 2021.04 東京医科歯科大学生体材料工学研究所
細胞機能解析プラットフォーム構築に向けたナノデバイスの開発

遠藤達郎, 柳田保子

生体医歯工学共同研究拠点成果報告書 2021.04 東京医科歯科大学生体材料工学研究所
細胞機能解析プラットフォーム構築に向けたナノデバイスの開発

遠藤達郎, 柳田保子

生体医歯工学共同研究拠点成果報告書 2021.04 東京医科歯科大学生体材料工学研究所
細胞機能解析プラットフォーム構築に向けたナノデバイスの開発

遠藤達郎, 柳田保子

生体医歯工学共同研究拠点成果報告書 2021.04 東京医科歯科大学生体材料工学研究所
細胞機能解析プラットフォーム構築に向けたナノデバイスの開発 Invited Domestic conference

遠藤達郎

生体医歯工学共同研究拠点 2020年度(令和2年度)成果報告会 2021.03

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Preparation of ionic liquid-based dye (IL-Dye) nanoemulsion (NE) for high-sensitivity ion sensing International conference

Kaho Maki, Ryoutarou Oishi, Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

RSC Tokyo International Conference 2020 2020.11

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An enzyme-responsive fluorescent ionic liquid-based nanosensor toward highly sensitive biosensing International conference

Ryoutarou Oishi, Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto

RSC Tokyo International Conference 2020 2020.11

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Size sorting of exosomes by tuning the thicknesses of the electric double layers in nanochannels International conference

Satoko Fujiwara, Kyojiro Morikawa, Tatsuro Endo, Hideaki Hisamoto, Kenji Sueyoshi

RSC Tokyo International Conference 2020 2020.11

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高精度な細胞外微粒子分級用マイクロ・ナノ流体デバイスの開発

藤原聡子, 金子紗也, 森川響二朗, 遠藤達郎, 久本秀明, 末吉健志

日本分析化学会講演要旨集 2020.09 (公社)日本分析化学会
多段階部分的注入アフィニティーキャピラリー電気泳動法による物質間相互作用解析

光野恵理子, 久本秀明, 遠藤達郎, 末吉健志

日本分析化学会講演要旨集 2020.09 (公社)日本分析化学会
プラズモニック結晶を用いた非染色PCR産物検出デバイスの開発

古川ふう大, 井上千種, 川崎大輝, 山田大空, 末吉健志, 久本秀明, 遠藤達郎

日本分析化学会講演要旨集 2020.09 (公社)日本分析化学会
高精度な細胞外微粒子分級用マイクロ・ナノ流体デバイスの開発

藤原聡子, 金子紗也, 森川響二朗, 遠藤達郎, 久本秀明, 末吉健志

日本分析化学会講演要旨集 2020.09 (公社)日本分析化学会
多段階部分的注入アフィニティーキャピラリー電気泳動法による物質間相互作用解析

光野恵理子, 久本秀明, 遠藤達郎, 末吉健志

日本分析化学会講演要旨集 2020.09 (公社)日本分析化学会
プラズモニック結晶を用いた非染色PCR産物検出デバイスの開発

古川ふう大, 井上千種, 川崎大輝, 山田大空, 末吉健志, 久本秀明, 遠藤達郎

日本分析化学会講演要旨集 2020.09 (公社)日本分析化学会
高精度な細胞外微粒子分級用マイクロ・ナノ流体デバイスの開発

藤原聡子, 金子紗也, 森川響二朗, 遠藤達郎, 久本秀明, 末吉健志

日本分析化学会講演要旨集 2020.09 (公社)日本分析化学会
多段階部分的注入アフィニティーキャピラリー電気泳動法による物質間相互作用解析

光野恵理子, 久本秀明, 遠藤達郎, 末吉健志

日本分析化学会講演要旨集 2020.09 (公社)日本分析化学会
プラズモニック結晶を用いた非染色PCR産物検出デバイスの開発

古川ふう大, 井上千種, 川崎大輝, 山田大空, 末吉健志, 久本秀明, 遠藤達郎

日本分析化学会講演要旨集 2020.09 (公社)日本分析化学会
プラズモニック結晶を用いた非染色PCR産物検出デバイスの開発

古川ふう大, 井上千種, 川崎大輝, 山田大空, 末吉健志, 久本秀明, 遠藤達郎

日本分析化学会講演要旨集 2020.09 (公社)日本分析化学会
高精度な細胞外微粒子分級用マイクロ・ナノ流体デバイスの開発

藤原聡子, 金子紗也, 森川響二朗, 遠藤達郎, 久本秀明, 末吉健志

日本分析化学会講演要旨集 2020.09 (公社)日本分析化学会
多段階部分的注入アフィニティーキャピラリー電気泳動法による物質間相互作用解析

光野恵理子, 久本秀明, 遠藤達郎, 末吉健志

日本分析化学会講演要旨集 2020.09 (公社)日本分析化学会
ナノフォトニクスを基盤技術とした細胞機能解析プラットフォームの開発

遠藤達郎, 柳田保子

生体医歯工学共同研究拠点成果報告書 2020.04 東京医科歯科大学生体材料工学研究所
ポリマー製フォトニック結晶を用いた培養細胞の薬剤刺激応答観察

遠藤達郎, 柳田保子

生体医歯工学共同研究拠点成果報告書 2020.04 東京医科歯科大学生体材料工学研究所
ナノフォトニクスを基盤技術とした細胞機能解析プラットフォームの開発

遠藤達郎, 柳田保子

生体医歯工学共同研究拠点成果報告書 2020.04 東京医科歯科大学生体材料工学研究所
ポリマー製フォトニック結晶を用いた培養細胞の薬剤刺激応答観察

遠藤達郎, 柳田保子

生体医歯工学共同研究拠点成果報告書 2020.04 東京医科歯科大学生体材料工学研究所
ポリマー製フォトニック結晶を用いた培養細胞の薬剤刺激応答観察

遠藤達郎, 柳田保子

生体医歯工学共同研究拠点成果報告書 2020.04 東京医科歯科大学生体材料工学研究所
ナノフォトニクスを基盤技術とした細胞機能解析プラットフォームの開発

遠藤達郎, 柳田保子

生体医歯工学共同研究拠点成果報告書 2020.04 東京医科歯科大学生体材料工学研究所
ナノフォトニクスを基盤技術とした細胞機能解析プラットフォームの開発

遠藤達郎, 柳田保子

生体医歯工学共同研究拠点成果報告書 2020.04 東京医科歯科大学生体材料工学研究所
ポリマー製フォトニック結晶を用いた培養細胞の薬剤刺激応答観察

遠藤達郎, 柳田保子

生体医歯工学共同研究拠点成果報告書 2020.04 東京医科歯科大学生体材料工学研究所
電気泳動フィルタリングに基づく酵素反応初速度直接測定法の開発

高尾隼空, 遠藤達郎, 久本秀明, 末吉健志

日本分析化学会講演要旨集 2019.08 (公社)日本分析化学会
簡便・迅速・高感度な1stepELISAへの応用を指向した酵素応答性疎水性色素液体の開発

大石綾太郎, 水田巽, 末吉健志, 遠藤達郎, 久本秀明

日本分析化学会講演要旨集 2019.08 (公社)日本分析化学会
キャピラリー分子ふるい電気泳動に基づく低分子標的アプタマー選抜法の開発

和田将英, 遠藤達郎, 久本秀明, 末吉健志

日本分析化学会講演要旨集 2019.08 (公社)日本分析化学会
Inkjet Printingを用いた1 step競合型イムノアッセイマイクロデバイスの開発

河相優子, 角田正也, 末吉健志, 遠藤達郎, 久本秀明

日本分析化学会講演要旨集 2019.08 (公社)日本分析化学会
Inkjet Printingを用いた1 step競合型イムノアッセイマイクロデバイスの開発

河相優子, 角田正也, 末吉健志, 遠藤達郎, 久本秀明

日本分析化学会講演要旨集 2019.08 (公社)日本分析化学会
電気泳動フィルタリングに基づく酵素反応初速度直接測定法の開発

高尾隼空, 遠藤達郎, 久本秀明, 末吉健志

日本分析化学会講演要旨集 2019.08 (公社)日本分析化学会
簡便・迅速・高感度な1stepELISAへの応用を指向した酵素応答性疎水性色素液体の開発

大石綾太郎, 水田巽, 末吉健志, 遠藤達郎, 久本秀明

日本分析化学会講演要旨集 2019.08 (公社)日本分析化学会
キャピラリー分子ふるい電気泳動に基づく低分子標的アプタマー選抜法の開発

和田将英, 遠藤達郎, 久本秀明, 末吉健志

日本分析化学会講演要旨集 2019.08 (公社)日本分析化学会
電気泳動フィルタリングに基づく酵素反応初速度直接測定法の開発

高尾隼空, 遠藤達郎, 久本秀明, 末吉健志

日本分析化学会講演要旨集 2019.08 (公社)日本分析化学会
簡便・迅速・高感度な1stepELISAへの応用を指向した酵素応答性疎水性色素液体の開発

大石綾太郎, 水田巽, 末吉健志, 遠藤達郎, 久本秀明

日本分析化学会講演要旨集 2019.08 (公社)日本分析化学会
キャピラリー分子ふるい電気泳動に基づく低分子標的アプタマー選抜法の開発

和田将英, 遠藤達郎, 久本秀明, 末吉健志

日本分析化学会講演要旨集 2019.08 (公社)日本分析化学会
Inkjet Printingを用いた1 step競合型イムノアッセイマイクロデバイスの開発

河相優子, 角田正也, 末吉健志, 遠藤達郎, 久本秀明

日本分析化学会講演要旨集 2019.08 (公社)日本分析化学会
Inkjet Printingを用いた1 step競合型イムノアッセイマイクロデバイスの開発

河相優子, 角田正也, 末吉健志, 遠藤達郎, 久本秀明

日本分析化学会講演要旨集 2019.08 (公社)日本分析化学会
電気泳動フィルタリングに基づく酵素反応初速度直接測定法の開発

高尾隼空, 遠藤達郎, 久本秀明, 末吉健志

日本分析化学会講演要旨集 2019.08 (公社)日本分析化学会
簡便・迅速・高感度な1stepELISAへの応用を指向した酵素応答性疎水性色素液体の開発

大石綾太郎, 水田巽, 末吉健志, 遠藤達郎, 久本秀明

日本分析化学会講演要旨集 2019.08 (公社)日本分析化学会
キャピラリー分子ふるい電気泳動に基づく低分子標的アプタマー選抜法の開発

和田将英, 遠藤達郎, 久本秀明, 末吉健志

日本分析化学会講演要旨集 2019.08 (公社)日本分析化学会
細胞の機械的応答観察を指向したポリマー製フォトニック結晶の開発

遠藤達郎, 柳田保子

生体医歯工学共同研究拠点成果報告書 2019.04 東京医科歯科大学生体材料工学研究所
細胞の機械的応答観察を指向したポリマー製フォトニック結晶の開発

遠藤達郎, 柳田保子

生体医歯工学共同研究拠点成果報告書 2019.04 東京医科歯科大学生体材料工学研究所
細胞の機械的応答観察を指向したポリマー製フォトニック結晶の開発

遠藤達郎, 柳田保子

生体医歯工学共同研究拠点成果報告書 2019.04 東京医科歯科大学生体材料工学研究所
細胞の機械的応答観察を指向したポリマー製フォトニック結晶の開発

遠藤達郎, 柳田保子

生体医歯工学共同研究拠点成果報告書 2019.04 東京医科歯科大学生体材料工学研究所

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Industrial Property Rights

検出装置、および、検出方法

遠藤達郎, 山田大空, 川崎大輝

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property_type：Patent

Application no：特願2021-151831

Announcement no：特開2023-044017

J-GLOBAL
被検出物質の検出キット、検出システムおよび検出方法

遠藤達郎, 山田大空, 川崎大輝, 小林奈緒

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property_type：Patent

Application no：特願2021-135765

Announcement no：特開2023-030564

J-GLOBAL
フォトニック結晶素子およびその製造方法、イオンセンサおよびそれを用いるイオン検出システム、ならびに、味物質センサおよびそれを用いる味物質検出システム

遠藤達郎, 森田考則

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property_type：Patent

Application no：特願2019-206561

Announcement no：特開2020-085905

J-GLOBAL
検査方法、検査用器具及び検査装置

赤木良教, 浅野元彦, 土居淳, 遠藤達郎

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property_type：Patent

Application no：JP2019030010

Publication no：WO2020-027197

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検査方法、検査用器具及び検査装置

赤木良教, 浅野元彦, 土居淳, 遠藤達郎

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property_type：Patent

Application no：JP2019030010

Patent/Registration no：特許第7345782号

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プラズモン共鳴構造体、それを用いたプラズモン共鳴センサおよびプラズモン共鳴測定システム、ならびに、プラズモン共鳴構造体の製造方法

遠藤達郎, 山田大空

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property_type：Patent

Application no：特願2018-116733

Announcement no：特開2019-219272

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フォトニック結晶素子およびそれを備えた分光システム、被検出物質の検出キットおよび検出システム、ならびに、フォトニック結晶素子の製造方法

遠藤達郎, 前野権一

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property_type：Patent

Application no：特願2018-107401

Announcement no：特開2019-211326

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フォトニック結晶素子およびその製造方法、ならびに、イオンセンサおよびそれを用いるイオン検出システム

遠藤達郎

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property_type：Patent
プラズモン共鳴構造体、それを用いたプラズモン共鳴センサおよびプラズモン共鳴測定システム、ならびに、プラズモン共鳴構造体の製造方法

遠藤達郎，山田大空

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property_type：Patent
フォトニック結晶素子およびそれを備えた分光システム、被検出物質の検出キットおよび検出システム、ならびに、フォトニック結晶素子の製造方法

遠藤達郎，前野権一

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property_type：Patent
分析プレート、分析方法、及び分析プレートの製造方法

遠藤達郎, 矢賀部裕

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property_type：Patent

Application no：特願2017-566938

Patent/Registration no：特許第6968365号

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シート、その製造方法およびシート作製用鋳型

遠藤達郎, 塚本啓司, 伊田寛之

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property_type：Patent
光学式センサー及び光学式センサーの製造方法

遠藤達郎, 福田真林

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property_type：Patent
検査用器具、検査装置及び検査方法

赤木良教, 乾延彦, 今村一彦, 河野隆昌, 野村　茂, 遠藤達郎

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property_type：Patent
測定用チップ、測定装置、および測定方法

遠藤達郎, 多田啓二, 山林　潤

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property_type：Patent
測定用チップ、測定装置、および測定方法

遠藤達郎, 多田啓二, 山林潤

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property_type：Patent

Application no：特願2015-035426

Announcement no：特開2016-156727

Patent/Registration no：特許第6676236号

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培養基材及び培養方法

赤木良教, 野村　茂, 鹿毛崇至, 河野隆昌, 今村一彦, 遠藤達郎

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property_type：Patent
アルコール濃度評価装置

山田誠，遠藤達郎

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property_type：Patent
光学センサー及び該センサーの作製方法

遠藤達郎, 安藝翔馬

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property_type：Patent
光測定装置

柳田保子, 今井泰徳, 初澤　毅, 遠藤達郎

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property_type：Patent
光学式センサーおよびその製造方法並びに光学式センサーを用いた検出方法

遠藤達郎, 高谷佳輝, 田中　覚

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property_type：Patent
バイオセンサ

初澤　毅, 遠藤達郎

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property_type：Patent
微小粒子単層の製造方法

遠藤達郎, 池田大輔, 滝澤　光, 龍野功幸

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property_type：Patent
バイオセンサー

遠藤達郎

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property_type：Patent
分析方法及び分析装置

民谷栄一, 永谷尚紀, 由比光子, 遠藤達郎, 田中　亮

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property_type：Patent
被検物質の検出方法、増感剤及びイムノクロマトグラフィー用キット

民谷栄一, 永谷尚紀, 由比光子, 遠藤達郎, 田中　亮

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property_type：Patent
物質の定量方法及び物質の定量デバイス

民谷栄一, 遠藤達郎, 村上佳裕

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property_type：Patent
非標識バイオチップ

民谷栄一, 遠藤達郎

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property_type：Patent

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Collaborative research (seeds) keywords

化学センサー開発
バイオセンサー開発
ナノテクノロジーを基盤技術としたデバイス作製

Outline of collaborative research (seeds)

生体計測用バイオセンシングデバイスの開発
ナノ構造を利用したバイオ/化学センサの開発
疾病診断用バイオセンサデバイスの開発
フォトニクス/エレクトロニクスを基盤技術としたバイオセンシングデバイスの開発

Grant-in-Aid for Scientific Research

Development of photonic crystal nanocavity arrays for epigenomic analysis

Grant-in-Aid for Scientific Research(B) 2024

Charge of on-campus class subject

構造解析演習

2024 Weekly class Undergraduate
応用化学実験2

2024 Weekly class Undergraduate
応用化学概論

2024 Weekly class Undergraduate
分析化学特論

2024 Weekly class Graduate school
物質化学生命系特別研究第1

2024 Intensive lecture Graduate school
物質化学生命系特別演習第1

2024 Intensive lecture Graduate school
物質化学生命系特別演習

2024 Intensive lecture Graduate school
応用化学実験III

2024 Intensive lecture Undergraduate
物質化学生命系特別演習

2023 Intensive lecture Graduate school
分析化学特論

2023 Weekly class Graduate school
物質化学生命系特別研究第1

2023 Intensive lecture Graduate school
物質化学生命系特別演習第1

2023 Intensive lecture Graduate school
応用化学実験III

2023 Weekly class Undergraduate
機器分析学

2023 Weekly class Undergraduate
応用化学実験1

2023 Weekly class Undergraduate
応用化学実験IV

2023 Weekly class Undergraduate
応用化学実験II

2023 Intensive lecture Undergraduate
応用化学実験V

2023 Weekly class Undergraduate
物質化学生命系特別研究

2023 Intensive lecture Graduate school
応用化学特論3

2023 Weekly class Graduate school
物質化学生命系特別研究第2

2023 Intensive lecture Graduate school
物質化学生命系特別演習第2

2023 Intensive lecture Graduate school
応用化学実験I

2022 Weekly class Undergraduate
応用化学実験III

2022 Weekly class Undergraduate
初年次ゼミナール

2022 Weekly class Undergraduate
物質化学生命系特別演習

2022 Intensive lecture Graduate school
物質化学生命系特別演習第1

2022 Intensive lecture Graduate school
物質化学生命系特別演習第2 （応用化学分野）

2022 Intensive lecture Graduate school
応用化学特論3 （応用化学分野）

2022 Weekly class Graduate school
機器分析学

2022 Weekly class Undergraduate
物理化学演習IA

2022 Weekly class Undergraduate
物質化学生命系特別研究（応用化学分野）

2022 Intensive lecture Graduate school
応用化学実験II

2022 Weekly class Undergraduate
応用化学実験IV

2022 Weekly class Undergraduate
応用化学実験V

2022 Weekly class Undergraduate
Exercises in Physical Chemistry IA

2021
Laboratory Exercises for Applied Chemistry II

2021 Practical Training
Laboratory Exercises for Applied Chemistry I

2021 Practical Training
Laboratory Exercises for Applied Chemistry V

2021 Practical Training
Laboratory Exercises for Applied Chemistry III

2021 Practical Training
Instrumental Methods of Analysis

2021
Laboratory Exercises for Applied Chemistry IV

2021 Practical Training
Advanced Analytical Chemistry in Technology

2021

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Number of papers published by graduate students

2023

Number of graduate students presentations：7
2022

Number of graduate students presentations：9
2021

Number of graduate students presentations：7
2020

Number of graduate students presentations：10
2019

Number of graduate students presentations：6

Number of instructed thesis, researches

2023

Number of instructed the graduation thesis：7

[Number of instructed the Master's Program] (previous term)：4 [Number of instructed the Master's Program] (letter term)：4
2022

Number of instructed the graduation thesis：7

[Number of instructed the Master's Program] (previous term)：4 [Number of instructed the Master's Program] (letter term)：2
2021

Number of instructed the graduation thesis：5

[Number of instructed the Master's Program] (previous term)：6 [Number of instructed the Master's Program] (letter term)：1
2020

Number of instructed the graduation thesis：5

[Number of instructed the Master's Program] (previous term)：6 [Number of instructed the Master's Program] (letter term)：1
2019

Number of instructed the graduation thesis：6

[Number of instructed the Master's Program] (previous term)：4

Academic Activities

電気学会論文誌Ｅ（センサ・マイクロマシン部門誌）

2011.10 - Now