Updated on 2024/03/22

写真a

 
KONDO TOMOHIRO
 
Organization
Graduate School of Veterinary Science Department of Veterinary Science Lecturer
School of Veterinary Science Department of Veterinary Science
Title
Lecturer
Affiliation
Institute of Veterinary Science
Contact information
メールアドレス

Position

  • Graduate School of Veterinary Science Department of Veterinary Science 

    Lecturer  2022.04 - Now

  • School of Veterinary Science Department of Veterinary Science 

    Lecturer  2022.04 - Now

Degree

  • 博士(獣医学) ( Osaka Prefecture University )

Professional Memberships

  • The Japan Society for Developmental Origins of Health and Disease

    2018.04 - Now   Domestic

  • 日本実験動物医学専門医協会

    2013.03 - Now

  • The Japanese Society of Veterinary Science

    2010.04 - Now   Domestic

  • Japanese Association for Laboratory Animal Medicine

    2010.04 - Now   Domestic

  • Japanese Association for Laboratory Animal Science

    2010.04 - Now   Domestic

  • The Japanese Teratology Society

    2010.04 - Now   Domestic

▼display all

Committee Memberships (off-campus)

  • 令和6年度プロポーザル事業企画提案事業者選定委員   大阪市健康局健康推進部  

    2024.01 - 2024.03 

  • 大阪市動物愛護推進会議 委員   大阪市健康局健康推進部  

    2023.04 - 2025.03 

  • 調査委員会委員   公私立大学実験動物施設協議会  

    2023.04 - 2024.03 

  • 委員   寝屋川市 動物愛護推進協議会  

    2023.04 - 2024.03 

  • 大阪市動物愛護推進会議 委員   大阪市健康局健康推進部 生活衛生課  

    2022.04 - 2023.03 

  • 委員   寝屋川市 動物愛護推進協議会  

    2022.04 - 2023.03 

▼display all

Papers

  • Oxygen-glucose deprivation-induced glial cell reactivity in the rat primary neuron-glia co-culture Reviewed

    INOUE Maiko, TANIDA Takashi, KONDO Tomohiro, TAKENAKA Shigeo, NAKAJIMA Takayuki

    Journal of Veterinary Medical Science   85 ( 8 )   799 - 808   2023.08( ISSN:09167250 ( eISSN:13477439

     More details

    Publishing type:Research paper (scientific journal)  

    <p>It has been demonstrated that <i>in vivo</i> brain ischemia induces activation and proliferation of astrocytes and microglia. However, the mechanism underlying the ischemia-induced activation and proliferation of these cells remains to be unclear. Oxygen-glucose deprivation (OGD), an <i>in vitro</i> ischemia mimic, has been extensively used to analyze the hypoxia response of various cell types. This study examined the OGD-induced changes in the expression level of astrocytes and microglia marker proteins and immunoreactivity for Ki-67, a marker protein for cell proliferation, using rat primary hippocampal neuron-glia co-culture (NGC) cells. Furthermore, OGD-induced changes in the expression of M1/M2 microglia phenotype-related genes were also examined. MTT assay indicated that 120 min of OGD decreased cell viability, and immunocytochemistry indicated that 120 min of OGD abolished most microtubule-associated protein 2 (MAP2)-immunopositive neurons. In contrast, glial fibrillary acidic protein (GFAP)-immunopositive astrocytes and ionized calcium-binding adapter protein-1 (Iba-1)-immunopositive microglia, and 2’,3’-cyclic nucleotide-3’-phosphodiesterase (CNPase)-immunopositive oligodendrocytes survived OGD. Western blot assays and double-immunofluorescent staining indicated that OGD increased the GFAP expression level and the Ki-67-immunopositive/GFAP-immunopositive cells’ ratio. Real-time PCR analysis showed that OGD altered M1 microglia phenotype-related genes. Specifically, OGD decreased the expression level of <i>CD32</i> and interleukin-1β (<i>IL-1β</i>) genes and increased that of the inducible nitric oxide synthase (<i>iNOS</i>) gene. Therefore, applying OGD to NGC cells could serve as a useful <i>in vitro</i> tool to elucidate the molecular mechanisms underlying brain ischemia-induced changes in GFAP expression, astrocyte proliferation, and M1 microglia phenotype-related gene expression.</p>

    DOI: 10.1292/jvms.23-0175

    PubMed

  • Novel intrauterine growth retardation model: effects of maternal subtotal nephrectomy on neonates. Reviewed

    Shoji Ogawa, Tamaki Yana, Tomohiro Kondo, Toshiya Okada

    The Journal of veterinary medical science   84 ( 9 )   1261 - 1264   2022.09

     More details

    Publishing type:Research paper (scientific journal)   International / domestic magazine:Domestic journal  

    Changes in body weight (BW), systolic blood pressure (SBP), and localization of renin in the kidneys of neonates born to normal mothers (C neonates) or to five-sixths (5/6) nephrectomized (2/3 left kidney and right kidney) mothers (Nx neonates) were studied. Maternal 5/6 nephrectomy caused weight loss in neonates but no differences in SBP or renin localization. Culling Nx neonates to a litter of 3 at 1 day after birth resulted in growth catching up with C neonates from 3 weeks old and increases in both SBP and renin-positive cells in neonatal kidney. These findings revealed that maternal 5/6 nephrectomy results in low-birth-weight neonates and that these neonates are at increased risk of metabolic syndrome by catch-up growth.

    DOI: 10.1292/jvms.22-0025

    PubMed

  • The expression and phosphorylation of SMAD3 protein in microglia and astrocytes of the rat hippocampus after transient global cerebral ischemia. Reviewed

    J Chem Neuroanat,   125   102146   2022.08

  • Changes in Smad1/5/9 expression and phosphorylation in astrocytes of the rat hippocampus after transient global cerebral ischemia Reviewed

    Takayuki Nakajima, Yuji Kunieda, Yusuke Takahashi, Yuki Tanaka, Tomohiro Kondo, Shigeo Takenaka.

    J. Chem. Neuroanat. 雑誌   113   2021.03

     More details

    Kind of work:Joint Work  

  • Transient forebrain ischemia induces impairment in cognitive performance prior to extensive neuronal cell death in Mongolian gerbil (Meriones unguiculatus). Reviewed

    Kondo Tomohiro, Yoshida Suguru, Nagai Hiroaki, Takeshita Ai, Mino Masaki, Morioka Hiroshi, Nakajima Takayuki, Kusakabe Ken Takeshi, Okada Toshiya

    JOURNAL OF VETERINARY SCIENCE   2018.07

     More details

    Kind of work:Joint Work  

  • Distribution of Smad mRNA and proteins in the rat brain Reviewed

    Nakajima Takayuki, Hata Ryusuke, Kunieda Yuji, Kondo Tomohiro

    JOURNAL OF CHEMICAL NEUROANATOMY   2018.07

     More details

    Kind of work:Joint Work  

  • Morphological analyses of the retinal photoreceptor cells in the nocturnally adapted owl monkeys. Reviewed

    Kuniyoshi Nobue, Yoshida Yuji, Itoh Yoshiki, Yokota Shin-ichi, Kuraishi Takeshi, Hattori Shosaku, Kondo Tomohiro, Yoshizawa Midori, Kai Chieko, Kiso Yasuo, Kusakabe Ken Takeshi.

    JOURNAL OF VETERINARY MEDICAL SCIENCE   2018.03

     More details

    Kind of work:Joint Work  

  • Evidence that endogenous formaldehyde produces immunogenic and atherogenic adduct epitopes. Reviewed

    Nakamura Jun, Shimomoto Takasumi, Collins Leonard B., Holley Darcy W., Zhang Zhenfa, Barbee Jenna M., Sharma Vyom, Tian Xu, Kondo Tomohiro, Uchida Koji, Yi Xianwen, Perkins Diana O., Willis Monte S., Gold Avram, Bultman Scott J.

    Sci Rep   2017.09

     More details

    Kind of work:Joint Work  

  • A Novel Spontaneous Mutation of BCAR3 Results in Extrusion Cataracts in CF#1 Mouse Strain. Reviewed

    Kondo T, Nakamori T, Nagai H, Takeshita A, Kusakabe KT, Okada T.

    Mamm Genome   2016.10

  • Maternal protein restriction that does not have an influence on the birthweight of the offspring induces morphological changes in kidneys reminiscent of phenotypes exhibited by intrauterine growth retardation rats. Reviewed

    Yuasa K, Kondo T, Nagai H, Mino M, Takeshita A, Okada T.

    Congenit Anom   2016.03

  • Effects of maternal subtotal nephrectomy on the development of the fetal kidney: A morphometric study. Reviewed

    Kondo T, Kitano-Amahori Y, Nagai H, Mino M, Takeshita A, Kusakabe KT, Okada T.

    Congenit Anom   2015.11

  • Proteomic analysis of the hippocampus in naïve and ischemic-preconditioned rat. Reviewed

    Nakajima T, Hata R, Kondo T, Takenaka S.

    J Neurol Sci   2015.11

  • Hereditary and Histologic Characteristics of the CF1/b cac Mouse Cataract Model. Reviewed

    Kondo T, Nagai H, Kawashima T, Taniguchi Y, Koyabu N, Takeshita A, Kusakabe KT, Okada T.

    Comp Med 雑誌   2014.10

     More details

    Kind of work:Joint Work  

  • Expression of transforming growth factor β and fibroblast growth factor 2 in the lens epithelium of Morioka cataract mice.

    Kondo T, Ishiga-Hashimoto N, Nagai H, Takeshita A, Mino M, Morioka H, Kusakabe KT, Okada T.

    Congenit Anom 雑誌   2014.05

     More details

    Kind of work:Joint Work  

  • Dynamics and reproductive effects of complement factors in the spontaneous abortion model of CBA/J×DBA/2 mice. Reviewed

    Takeshita A, Kusakabe KT, Hiyama M, Kuniyoshi N, Kondo T, Kano K, Kiso Y, Okada T.

    Immunobiology 雑誌   2014.05

     More details

    Kind of work:Joint Work  

  • Effects of transient forebrain ischemia on the hippocampus of the Mongolian Gerbil (Meriones unguiculatus): An immunohistochemical study. Reviewed

    Okada T, Kataoka Y, Takeshita A, Mino M, Morioka H, Kusakabe KT, Kondo T.

    Zoolog Sci 雑誌   2013.06

     More details

    Kind of work:Joint Work  

  • An increase in apoptosis and reduction in aB-crystallin expression levels in the lens underlie the cataractogenesis of Morioka cataract (MCT) mice. Reviewed

    Kondo T, Ishiga-Hashimoto N, Nagai H, Takeshita A, Mino M, Morioka H, Kusakabe KT, Okada T.

    Med Mol Morphol   44   221 - 227   2011

     More details

    Kind of work:Joint Work  

  • Elevation of adipsin, a complement activating factor, in the mouse placenta during spontaneous abortion. Reviewed

    Takeshita A, Kondo T, Okada T, Kusakabe KT.

    J Reprod Dev   56   508 - 514   2010

     More details

    Kind of work:Joint Work  

  • Compensatory renal growth in uninephrectomized immature rats: Proliferative activity and epidermal growth factor. Reviewed

    Okada T, Omoto-Kitao M, Mukamoto M, Nakamura J, Mino M, Kondo T, Takeshita A, Kusakabe KT, Kato K.

    J Vet Med Sci   72   975 - 980   2010

     More details

    Kind of work:Joint Work  

  • Effects of low protein intake on the development of the remaining kidney in subtotally nephrectomized immature rats: Expression of inducible and endothelial NO synthase. Reviewed

    Mino M, Ihara H, Kozaki S, Kondo T, Takeshita A, Kusakabe KT, Okada T.

    Med Mol Morphol   43   116 - 122   2010

     More details

    Kind of work:Joint Work  

  • Novel cataract mouse model using ddY strain: Hereditary and histological characteristics. Reviewed

    Kondo T, Nagai H, Morioka H, Kusakabe KT, Okada T.

    J Vet Med Sci   72   203 - 209   2010

     More details

    Kind of work:Joint Work  

  • DNA microarray analysis with a mouse model for endometriosis and validation of candidate factors with human adenomyosis. Reviewed

    Kusakabe KT, Abe H, Kondo T, Kato K, Okada T, Otsuki Y.

    J Reprod Immunol   85   149 - 160   2010

     More details

    Kind of work:Joint Work  

  • Regulation of complement activity via the alternative pathway in placentas of mouse spontaneous abortions. Reviewed

    Takeshita A, Nagaishi S, Kondo T, Okada T, Kusakabe KT.

    J Vet Med Sci   72   1375 - 1377   2010

     More details

    Kind of work:Joint Work  

▼display all

Books and Other Publications

  • 獣医発生学第2版

    木曾康郎監修( Role: Joint author)

    学窓社  2019.02 

  • カラーアトラス動物発生学

    山本雅子, 谷口和美 他( Role: Joint author)

    緑書房  2014 

  • DNA Microarrays

    Ken Kusakabe, Ai Takeshita, Hideaki Abe, Tomohiro Kondo, Toshiya Okada.( Role: Joint author)

    Nova Science Publishers, Inc  2012 

     More details

    Responsible for pages:347- 361  

  • Renal Failure

    Toshiya Okada, Yoko Kitano-Amahori, Masaki Mino, Tomohiro Kondo, Ai Takeshita and Ken-Takeshi Kusakabe( Role: Joint author)

    INTECH  2012  ( ISBN:979-953-307-465-7

     More details

    Responsible for pages:81-104  

Grant-in-Aid for Scientific Research

  • Functional significance of ischemia-induced TGF-beta/Smad signaling in glial cells

    Grant-in-Aid for Scientific Research(C)  2026

  • 5/6腎臓摘出母体から生まれた子の成長に関する研究:新たな子癇前症モデルの開発

    Grant-in-Aid for Scientific Research(C)  2025

  • Functional significance of ischemia-induced TGF-beta/Smad signaling in glial cells

    Grant-in-Aid for Scientific Research(C)  2025

  • 5/6腎臓摘出母体から生まれた子の成長に関する研究:新たな子癇前症モデルの開発

    Grant-in-Aid for Scientific Research(C)  2024

  • Functional significance of ischemia-induced TGF-beta/Smad signaling in glial cells

    Grant-in-Aid for Scientific Research(C)  2024

Charge of on-campus class subject

  • 実験動物学実習

    2024   Weekly class   Undergraduate

  • 獣医療倫理・動物福祉学

    2024   Weekly class   Undergraduate

  • 実験動物学・遺伝育種学

    2024   Weekly class   Undergraduate

  • 小動物基礎臨床実習

    2024   Intensive lecture   Undergraduate

  • 実験動物学特論

    2024   Intensive lecture   Graduate school

  • 動物倫理特別講義

    2024   Intensive lecture   Graduate school

  • 動物行動解析学

    2024   Intensive lecture   Undergraduate

  • 獣医科学英語演習

    2024   Intensive lecture   Undergraduate

  • 生物統計学演習

    2024   Intensive lecture   Undergraduate

  • 実験動物学

    2024   Intensive lecture   Undergraduate

  • 研究公正A

    2024   Intensive lecture   Graduate school

  • 実験動物学実習

    2023   Weekly class   Undergraduate

  • 獣医療倫理・動物福祉学

    2023   Weekly class   Undergraduate

  • 実験動物学・遺伝育種学

    2023   Weekly class   Undergraduate

  • 小動物基礎臨床実習

    2023   Intensive lecture   Undergraduate

  • 獣医科学特別研究1

    2023   Intensive lecture   Graduate school

  • 獣医科学特別研究1

    2023   Intensive lecture   Graduate school

  • 国際・国内インターンシップ

    2023   Intensive lecture   Graduate school

  • 獣医科学特別研究1

    2023   Intensive lecture   Graduate school

  • 獣医科学特別演習1

    2023   Intensive lecture   Graduate school

  • 獣医科学演習

    2023   Intensive lecture   Graduate school

  • 実験動物学特論

    2023   Intensive lecture   Graduate school

  • 臨床基礎実習

    2023   Weekly class   Undergraduate

  • 動物行動解析学

    2023   Intensive lecture   Undergraduate

  • 生物統計学演習

    2023   Weekly class   Undergraduate

  • 実験動物学

    2023   Intensive lecture   Undergraduate

  • 研究公正A

    2023   Intensive lecture   Graduate school

  • 遺伝育種学

    2023   Intensive lecture   Undergraduate

  • 疾患モデル動物学

    2023   Intensive lecture   Undergraduate

  • 実験動物学実習

    2023   Intensive lecture   Undergraduate

  • 獣医療倫理・動物福祉学

    2023   Intensive lecture   Undergraduate

  • 生物学実験

    2023   Intensive lecture   Graduate school

  • 獣医科学特別演習1

    2023   Intensive lecture   Graduate school

  • 獣医科学ミニレビュー

    2023   Intensive lecture   Graduate school

  • 獣医科学演習

    2023   Intensive lecture   Graduate school

  • 国際・国内インターンシップ

    2023   Intensive lecture   Graduate school

  • 獣医科学特別研究1

    2023   Intensive lecture   Graduate school

  • 発生学

    2023   Weekly class   Undergraduate

  • 獣医体験演習

    2023   Weekly class   Undergraduate

  • 基礎実験動物学

    2023   Weekly class   Undergraduate

  • Special Lecture: Integrated Structural Biosciences C

    2021    

  • Animal Behavior Analysis

    2021    

  • Pathological Animal Model Science

    2021    

  • Fundamental Experiments of Biology

    2021   Practical Training  

  • Veterinary Medical Ethics, Animal Welfare

    2021    

  • Practice in Laboratory Animal Science

    2021   Practical Training  

  • Basic clinical practices

    2021   Practical Training  

  • Practice in Biological Statistics

    2021    

  • Animal Breeding and Genetics

    2021    

  • Veterinary Embryology

    2021    

  • Laboratory Animal Science

    2021    

▼display all