Updated on 2024/05/01

写真a

 
KAMITANI SHIGEKI
 
Organization
Graduate School of Human Life and Ecology Division of Human Life and Ecology Professor
School of Human Life and Ecology Department of Nutrition
Title
Professor
Affiliation
Institute of Human Life and Ecology
Contact information
メールアドレス
Affiliation campus
Habikino Campus

Position

  • Graduate School of Human Life and Ecology Division of Human Life and Ecology 

    Professor  2022.04 - Now

  • School of Human Life and Ecology Department of Nutrition 

    Professor  2022.04 - Now

Degree

  • 博士(医学) ( Chiba University )

  • 理学修士 ( Kyoto University )

  • 工学士 ( Osaka Prefecture University )

Research Areas

  • Life Science / Food sciences

  • Humanities & Social Sciences / Family and consumer sciences, and culture and living

  • Life Science / Structural biochemistry  / Structural biochemistry

  • Life Science / Cell biology  / Cellular biology

  • Life Science / Molecular biology  / Molecular biology

  • Life Science / Bacteriology  / Bacteriology

  • Life Science / Food sciences

  • Life Science / Bacteriology

  • Life Science / Social dentistry

  • Life Science / Nutrition science and health science

  • Humanities & Social Sciences / Family and consumer sciences, and culture and living

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Research Interests

  • 生活習慣病

  • 口腔細菌叢

  • Periodontitis

  • crystal structure

  • host specificity

  • bacterial toxin

  • virulence factor

  • pathogenic bacteria

  • virulence factor

  • 生活習慣病

  • periodontitis

  • antibacterial activity

  • oral flora

  • メダカ

Research subject summary

  • 歯周病原細菌に対する抗菌作用を持つ食品成分の探索

  • 生活習慣病と歯周病現細菌と病態モデルの作製と応用

  • ライフステージと口腔細菌叢の構造との関連の解析

  • 細菌の病原因子の作用機構の解明

Research Career

  • 歯周病原細菌に対する抗菌作用を持つ食品成分の探索

    Joint Research in Japan

    2013.04 - Now 

  • 生活習慣病と歯周病現細菌と病態モデルの作製と応用

    Joint Research in Japan

    2016.04 - Now 

  • ライフステージと口腔細菌叢の構造との関連の解析

    Joint Research in Japan

    2016.04 - Now 

  • 歯周原細菌の病原因子の解析

    歯周病、病原因子  Joint Research in Japan

    2013.04 - Now 

Professional Memberships

  • 日本栄養・食糧学会

    2015.04 - Now   Domestic

  • 日本細菌学会

    2004 - Now   Domestic

  • 毒素シンポジウム

    2004 - Now   Domestic

  • 日本生化学会

    1990 - Now   Domestic

  • 日本分子生物学会

    1990 - Now   Domestic

  • 日本化学会

    1989 - 1992   Domestic

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Papers

  • Gαq modulates the energy metabolism of osteoclasts.

    Sushmita Chakraborty, Bianca Handrick, Dayoung Yu, Konrad A Bode, Anna Hafner, Judith Schenz, Dominik Schaack, Florian Uhle, Taro Tachibana, Shigeki Kamitani, Thomas Vogl, Katharina F Kubatzky

    Frontiers in cellular and infection microbiology   12   1016299 - 1016299   2023.01

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    INTRODUCTION: The bacterial protein toxin Pasteurella multocida toxin (PMT) mediates RANKL-independent osteoclast differentiation. Although these osteoclasts are smaller, their resorptive activity is high which helps in efficient destruction of nasal turbinate bones of pigs. METHODS: The proteome of bone marrow-derived macrophages differentiated into osteoclasts with either RANKL or PMT was analysed. The results were verified by characterizing the metabolic activity using Seahorse analysis, a protein translation assay, immunoblots, real-time PCR as well as flow cytometry-based monitoring of mitochondrial activity and ROS production. A Gαq overexpression system using ER-Hoxb8 cells was used to identify Gαq-mediated metabolic effects on osteoclast differentiation and function. RESULTS: PMT induces the upregulation of metabolic pathways, which included strong glycolytic activity, increased expression of GLUT1 and upregulation of the mTOR pathway. As OxPhos components were expressed more efficiently, cells also displayed increased mitochondrial respiration. The heterotrimeric G protein Gαq plays a central role in this hypermetabolic cell activation as it triggers mitochondrial relocalisation of pSerSTAT3 and an increase in OPA1 expression. This seems to be caused by a direct interaction between STAT3 and OPA1 resulting in enhanced mitochondrial respiration. Overexpression of Gαq mimicked the hypermetabolic phenotype observed for PMT-induced osteoclasts and resulted in higher glycolytic and mitochondrial activity as well as increased bone resorptive activity. In addition, rheumatoid arthritis (RA) patients showed an increase in GNAQ expression, especially in the synovial fluid. DISCUSSION: Our study suggests that Gαq plays a key role in PMT-induced osteoclastogenesis. Enhanced expression of GNAQ at the site of inflammation in RA patients indicates its pathophysiological relevance in the context of inflammatory bone disorders.

    DOI: 10.3389/fcimb.2022.1016299

    PubMed

  • Analysis of the Effects of Food Additives on Porphyromonas gingivalis. Reviewed

    Mai Shinohara, Miki Maetani, Chiharu Kitada, Yasuko Nishigami, Ayaka Yazawa, Shigeki Kamitani

    Pathogens (Basel, Switzerland)   11 ( 1 )   2022.01

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    This study aims to investigate six food additives (octanoic acid, decanoic acid, acesulfame K, aspartame, saccharin, and sucralose) used in foods for the elderly or people with dysphagia because of the effect of these food additives on Porphyromonas gingivalis (P. gingivalis), which is a keystone pathogen of periodontal diseases. The growth of P. gingivalis was inhibited by 5 mM octanoic acid, 1.25 mM decanoic acid, 1.25% acesulfame K, 0.0625% aspartame, 0.03125% saccharin, and 0.625% sucralose. In addition, these food additives showed bactericidal activity for planktonic P. gingivalis (5 mM octanoic acid, 5 mM decanoic acid, 0.25% aspartame, 0.25% saccharin, and 5% sucralose). Moreover, biofilm formation was inhibited by 10 mM octanoic acid, 10 mM decanoic acid, 10% acesulfame K, 0.35% aspartame, 0.5% saccharin, and 7.5% sucralose. Moreover, the same concentration of these food additives without aspartame killed P. gingivalis in the biofilm. Aspartame and sucralose did not show cytotoxicity to human cell lines at concentrations that affected P. gingivalis. These findings may be useful in clarifying the effects of food additives on periodontopathogenic bacteria.

    DOI: 10.3390/pathogens11010065

    PubMed

  • Analysis of Chewing and Preference Characteristics of Raphanus sativus L. cv. Tanabe after Cooking using Different Methods. Reviewed

    Yamashita E, Takao R, Ogawa Y, Yoshida Y, Kamitani S.

    Jpn J Health Fit Nutr 雑誌   25・26 ( 1 )   1-8   2021

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    Authorship:Last author   Publishing type:Research paper (scientific journal)   Kind of work:Joint Work   International / domestic magazine:Domestic journal  

  • Crystal structure of pathogenic Staphylococcus aureus lipase complex with the anti-obesity drug orlistat. Reviewed

    Kengo Kitadokoro, Mutsumi Tanaka, Takaaki Hikima, Yukiko Okuno, Masaki Yamamoto, Shigeki Kamitani

    Scientific reports   10 ( 1 )   5469 - 5469   2020.03

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Staphylococcus aureus lipase (SAL), a triacylglycerol esterase, is an important virulence factor and may be a therapeutic target for infectious diseases. Herein, we determined the 3D structure of native SAL, the mutated S116A inactive form, and the inhibitor complex using the anti-obesity drug orlistat to aid in drug development. The determined crystal structures showed a typical α/β hydrolase motif with a dimeric form. Fatty acids bound near the active site in native SAL and inactive S116A mutant structures. We found that orlistat potently inhibits SAL activity, and it covalently bound to the catalytic Ser116 residue. This is the first report detailing orlistat-lipase binding. It provides structure-based information on the production of potent anti-SAL drugs and lipase inhibitors. These results also indicated that orlistat can be repositioned to treat bacterial diseases.

    DOI: 10.1038/s41598-020-62427-8

    PubMed

  • 噴門形成術後のダンピング症状に対してミルクの粘度調整が有効であったCHARGE症候群の一症例

    花井 美夢, 西本 裕紀子, 麻原 明美, 加嶋 倫子, 伊藤 真緒, 黒川 通典, 神谷 重樹, 岡田 洋介, 錦戸 知喜, 大沼 真輔, 惠谷 ゆり, 位田 忍

    New Diet Therapy   35 ( 3 )   3 - 9   2019.12( ISSN:0910-7258

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    症例は1歳4ヵ月男児で、CHARGE症候群に罹患しており、嚥下障害、食道裂孔ヘルニア、胃食道逆流のため、生後4ヵ月時に胃瘻造設、噴門形成術が施行された。1歳3ヵ月時に呼吸器感染により当院に入院となり、胃瘻からミルク(濃度14.7%)200mL×5回を各1時間で注入して栄養管理を行っていた。しかし、感染改善後も活気が乏しく、ミルク注入30分後に簡易血糖測定器を用いて血糖測定を行ったところ、2時間後に血糖値118mg/dLとなり、後期ダンピング症候群と診断された。治療方針として、増粘剤を用いてミルクの粘度調整を行い、血糖コントロールすることとした。ミルク200mLに対してトロミ調整食品「トロミスマイル」1.2g(容量比0.6%)を用いて粘度調整を行い、ポンプを用いて1時間で注入したところ、30分後の血糖値は200mg/dLとなった。さらにトロミスマイル1.4g(容量比0.7%)に増量し、その後5日間の各注入30分後の血糖値は中央値108mg/dLで安定して経過した。

  • Method for absolute quantification of microbial communities by using both microarrays and competitive PCR. Reviewed

    Ayaka Yazawa, Shigeki Kamitani, Naoyuki Togawa

    Journal of microbiological methods   165   105718 - 105718   2019.10( ISSN:0167-7012

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    International / domestic magazine:International journal  

    Methods for the robust quantification of bacterial communities are still under development. In this context, the present study aimed to evaluate a method combining competitive PCR (cPCR) and microarray assays for the determination of absolute content of total bacteria and individual bacterial species in samples. For this, a competitor DNA for cPCR and microarrays containing three types of DNA probes was prepared. A calibration curve was generated with genomic DNA samples as standards, which was then utilized for cPCR-based determination of the total number (in moles) of 16S rRNA genes in other bacterial samples. Moreover, scatter plots of species-specific probes versus total bacteria probe for each genomic DNA of known concentration was fit to the regression model, and the obtained slope value was defined as the hybridization affinity ratio. The cPCR assay was performed for both a commercially available mixed genomic DNA sample and human oral bacterial DNA samples, and the total number of moles of 16S rRNA genes was determined. These values were distributed among each species on the basis of the signal intensities of species-specific probes and the hybridization affinity ratio. The total number of bacterial genomes and those of individual species were determined by dividing the copy number of 16S rRNA genes per genome. The obtained results were confirmed by quantitative real-time PCR (qPCR). For values of >1 × 102 copies determined by qPCR, the ratio of the values measured by DNA chips to by qPCR was 1.53-fold on average and <2.6-fold for all data. These results show that the combined method of cPCR and microarray is useful to quantify the absolute numbers of several types of bacteria in a sample at one time.

    DOI: 10.1016/j.mimet.2019.105718

    PubMed

  • 日本人母子の歯周病原性細菌の分布と生活習慣(Distribution of periodontal pathogens and lifestyle habits of Japanese mothers and their children)

    Yazawa Ayaka, Okuno Yuko, Asao Sakiko, Maetani Miki, Shinagawa Hideo, Kawase Hiroki, Yoshioka Machiko, Kamitani Shigeki

    International Journal of Analytical Bio-Science   7 ( 3 )   49 - 58   2019.09( ISSN:2187-7912

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    小児の歯周病原性細菌(PP)の感染経路を明らかにするため、PPの分布と生活習慣(喫煙者の存在、歯磨き習慣、母子間での食物や食器の共有)との関係を分析した。93組の母子の歯垢検体から8種の細菌の存在を調べた結果、小児におけるFusobacterium nucleatum、Campylobacter rectusおよびTannerella forsythiaの存在が母親における存在と密接に関連していた。家庭内の喫煙者の割合は44.1%で、喫煙者は殆どが父親であった。母親(2.1±0.6回)と子供(1.7±0.7回)の歯磨き回数との間、ならびに子供と食器を共有する母親(62%)と食物を共有する母親(69%)との間に、有意な相関関係があった。しかし、PPの分布と家庭内の喫煙者の存在、歯磨き習慣、または母親との食物や食器の共有との間には相関関係はなかった。

  • 日本人母子の歯周病原性細菌の分布と生活習慣(Distribution of periodontal pathogens and lifestyle habits of Japanese mothers and their children)

    Yazawa Ayaka, Okuno Yuko, Asao Sakiko, Maetani Miki, Shinagawa Hideo, Kawase Hiroki, Yoshioka Machiko, Kamitani Shigeki

    International Journal of Analytical Bio-Science   7 ( 3 )   49 - 58   2019.09( ISSN:2187-7912

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    小児の歯周病原性細菌(PP)の感染経路を明らかにするため、PPの分布と生活習慣(喫煙者の存在、歯磨き習慣、母子間での食物や食器の共有)との関係を分析した。93組の母子の歯垢検体から8種の細菌の存在を調べた結果、小児におけるFusobacterium nucleatum、Campylobacter rectusおよびTannerella forsythiaの存在が母親における存在と密接に関連していた。家庭内の喫煙者の割合は44.1%で、喫煙者は殆どが父親であった。母親(2.1±0.6回)と子供(1.7±0.7回)の歯磨き回数との間、ならびに子供と食器を共有する母親(62%)と食物を共有する母親(69%)との間に、有意な相関関係があった。しかし、PPの分布と家庭内の喫煙者の存在、歯磨き習慣、または母親との食物や食器の共有との間には相関関係はなかった。

  • BspR/BtrA, an Anti-σ Factor, Regulates the Ability of <i>Bordetella bronchiseptica</i> To Cause Cough in Rats. Reviewed

    Nakamura K, Shinoda N, Hiramatsu Y, Ohnishi S, Kamitani S, Ogura Y, Hayashi T, Horiguchi Y

    mSphere   4 ( 2 )   2019.04

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    Publishing type:Research paper (scientific journal)  

    Bordetella pertussis, B. parapertussis, and B. bronchiseptica cause respiratory infections, many of which are characterized by coughing of the infected hosts. The pathogenesis of the coughing remains to be analyzed, mainly because there were no convenient infection models of small animals that replicate coughing after Bordetella infection. Here, we present a coughing model of rats infected with B. bronchiseptica. Rats, which are one of natural hosts of B. bronchiseptica, were readily infected with the organisms and showed frequent coughing. B. pertussis also caused coughing in rats, which is consistent with previous reports, but the cough response was less apparent than the B. bronchiseptica-induced cough. By using the rat model, we demonstrated that adenylate cyclase toxin, dermonecrotic toxin, and the type III secretion system are not involved in cough production, but BspR/BtrA (different names for the same protein), an anti-σ factor, regulates the production of unknown factor(s) to cause coughing. Rat coughing was observed by inoculation of not only the living bacteria but also the bacterial lysates. Infection with bspR (btrA)-deficient strains caused significantly less frequent coughing than the wild type; however, intranasal inoculation of the lysates from a bspR (btrA)-deficient strain caused coughing similarly to the wild type, suggesting that BspR/BtrA regulates the production of the cough factor(s) only when the bacteria colonize host bodies. Moreover, the cough factor(s) was found to be heat labile and produced by B. bronchiseptica in the Bvg phase. We consider that our rat model provides insight into the pathogenesis of cough induced by the Bordetella infection. +

    DOI: 10.1128/mSphere.00093-19

    PubMed

  • Structural insights into the unique polylactate-degrading mechanism of Thermobifida alba cutinase. Reviewed

    Kitadokoro K, Kakara M, Matsui S, Osokoshi R, Thumarat U, Kawai F, Kamitani S

    The FEBS journal   286 ( 11 )   2087 - 2098   2019.02( ISSN:1742-464X

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  • ‘田辺’ダイコンの根部の物性および糖含量の特性 Reviewed

    山下 絵美, 高井 雄一郎, 北田 康祐, 山崎 基嘉, 神谷 重樹

    新近畿中国四国農業研究   2 ( 2 )   1 - 12   2019.01

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.24755/westernagrires.2.0_1

  • Method for absolute quantification of microbial communities by using both microarrays and competitive PCR Reviewed

    Ayaka Yazawa, Shigeki Kamitani, Naoyuki Togawa.

    Journal of Microbiological Methods 雑誌   2019

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    Kind of work:Joint Work  

  • Distribution of periodontal pathogens and lifestyle habits of Japanese mothers and their children Reviewed

    Ayaka YAZAWA1, Yuko OKUNO, Sakiko ASAO, Miki MAETANI, Hideo SHINAGAWA2, Hiroki KAWASE, Machiko YOSHIOKA, and Shigeki KAMITANI

    International Journal of Analytical Bio-Science 雑誌   2019

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    Kind of work:Joint Work  

  • Purification, kinetic characterization, crystallization, and crystallographic study of Staphylococcus aureus lipase. Reviewed

    Tanaka M, Kamitani S, Kitadokoro K.

    Acta Crystallographica 雑誌   F74   567 - 570   2018

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    Kind of work:Joint Work  

  • Influence of Pasteurella multocida Toxin on the differentiation of dendritic cells into osteoclasts. Reviewed

    Chakrabortya S, Kloosa B, Roetza N, Schmidta S, Eigenbroda T, Kamitani S, and Kubatzky KF.

    Immunol 雑誌   223   142 - 150   2018

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    Kind of work:Joint Work  

  • Expression, Purification and Crystallization of Thermostable Mutant of Cutinase Est1 from Thermobifida alba. Reviewed

    Kitadokoro K, Matsui S, Osokoshi R, Nakata K, Kamitani S.

    Advs Biosci Biotechnol 雑誌   9   215 - 223   2018

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    Kind of work:Joint Work  

  • Association between the Porphyromonas gingivalis fimA type II genotype and the nutritional intake of elderly women. Reviewed

    Yazawa A, Maetani M, Takemura A, Kamitani S.

    J Life Sci Res 雑誌   15   1 - 7   2017

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    Kind of work:Joint Work  

    Repository URL: http://hdl.handle.net/10466/16082

  • Detection of genes expressed in Bordetella bronchiseptica colonizing rat trachea by in vivo expressed-tag immunoprecipitation method. Reviewed

    Abe H, Kamitani S, Fukui-Miyazaki A, Shinzawa N, Nakamura K, and Horiguchi Y.

    Microbiol Immunol 雑誌   59   249 - 261   2015

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    Kind of work:Joint Work  

  • Induction of neutral lipid-containing granules by staphylococcal lipase from clinical isolates. Reviewed

    Kamitani, S., Miyake, M., Hatano, M., Yutsudo, Y., Minamide, M., Kato, I., and Noda, M.

    SOJ Microbiol Infect Dis 雑誌   2 ( 2 )   1 - 7   2014.05

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    Kind of work:Joint Work  

  • 食中毒を引き起こすウェルシュ菌エンテロトキシンCPE の構造生物学的研究 Reviewed

    北所健吾,西村昂亮, 神谷重樹, 堀口安彦

    日本結晶学会誌 雑誌 日本結晶学会   55   223 - 229   2013.07

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    Kind of work:Joint Work  

  • Substrate specificity of Pasteurella multocida toxin for _ subunits of heterotrimeric G proteins. Reviewed

    Orth, J.H., Fester, I., Siegert, P., Weise, M., Lanner, U., Kamitani, S., Tachibana, T., Wilson, B.A., Schlosser, A., Horiguchi, Y., and Aktories, K.

    FASEB J 雑誌   27   832 - 842   2013.02

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    Kind of work:Joint Work  

  • IFN-γ elicits macrophage autophagy via the p38 MAPK signaling pathway. Reviewed

    Matsuzawa T, Kimb B-H, Kamitani S, Miyake M, and MacMicking JD.

    J Immunol 雑誌   189   813 - 818   2012

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    Kind of work:Joint Work  

  • Differences Between Group X and Group V Secretory Phospholipase A2 in Lipolytic Modification of Lipoproteins. Reviewed

    Kamitani S, Yamada K, Yamamoto S, Ishimoto Y, Ono T, Saiga A, and Hanasaki K.

    Cell Mol Biol Lett 雑誌   17   459 - 479   2012

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    Kind of work:Joint Work  

  • Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibody recognizing the deamidated alpha subunit of the heterotrimeric GTPase Gq. Reviewed

    Kamitani S, Ao S, Toshima H, Tachibana T, Hashimoto M, Kitadokoro K, Fukui-Miyazaki A, Abe H, and Horiguchi Y.

    FEBS J 雑誌   278   2702 - 2712   2011

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    Kind of work:Joint Work  

  • Bordetella dermonecrotic toxin binds to target cells via the N-terminal 30 amino acids. Reviewed

    Fukui-Miyazaki A, Ohnishi S, Kamitani S, Abe H, and Horiguchi Y.

    Microbiol Immunol 雑誌   55   154 - 159   2011

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    Kind of work:Joint Work  

  • Crystal Structure of Clostridium perfringens enterotoxin displays features of β-pore-forming toxins. Reviewed

    Kitadokoro K, Nishimura K, Kamitani S, Fukui-Miyazaki A, Toshima H, Abe H, Kamata Y, Sugita-Konishi Y, Yamamoto S, Karatani H, and Horiguchi Y.

    J Biol Chem 雑誌   286   2011

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    Kind of work:Joint Work  

  • Association of Bordetella dermonecrotic toxin with the extracellular matrix. Reviewed

    Fukui-Miyazaki A, Kamitani S, Miyake M, and Horiguchi Y.

    BMC Microbiology 雑誌   10   2010

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    Kind of work:Joint Work  

  • Clostridium perfringens Enterotoxin Interacts with Claudins via Electrostatic Attraction Reviewed

    Kimura J, Abe H, Kamitani S, Toshima H, Fukui A, Miyake M, Kamata Y, Sugita-Konishi Y, Yamamoto S, and Horiguchi Y.

    J Biol Chem 雑誌   285   401 - 408   2010

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    Kind of work:Joint Work  

  • Characterization of the membrane-targeting C1 domain in Pasteurella multocida toxin. Reviewed

    Kamitani S, Kitadokoro K, Miyazawa M, Toshima H, Fukui A, Abe H, Miyake M, and Horiguchi Y.

    J Biol Chem 雑誌   285   25467 - 25475   2010

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    Kind of work:Joint Work  

  • Pasteurella multocida由来の細菌毒素の細胞内機能領域の構造と機能解析 Reviewed

    北所 健悟, 神谷 重樹, 堀口 安彦

    日本結晶学会誌 雑誌   50   187 - 193   2008

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    Kind of work:Joint Work  

  • A colorimetric assay for studying 1 effector secretion through the bacterial type III secretion system. Reviewed

    Miyake M, Sakane S, Kobayashi C, Hanajima-Ozawa M, Fukui A, Kamitani S, and Horiguchi Y.

    FEMS Microbiol Lett 雑誌   278   36 - 42   2008

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    Kind of work:Joint Work  

  • The morphological changes in cultured cells caused by Bordetella pertussis adenylate cyclase toxin. Reviewed

    Ohnishi H, Miyake M, Kamitani S, Horiguchi Y.

    FEMS Microbiol Lett 雑誌   279   174 - 179   2008

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    Kind of work:Joint Work  

  • Crystal structure of the intracellular active region of Pasteurella multocida toxin: a membrane target domain and a catalytic domain with a cysteine protease-like catalytic triad. Reviewed

    Kitadokoro K*, Kamitani S*, Miyazawa M, Fukui A, Miyake M, and Horiguchi Y.(*: These authors are equally contributed.)

    Proc Natl Acad Sci USA 雑誌   104   5139 - 5144   2007

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    Kind of work:Joint Work  

  • The Effects of Long-term Smoking on Endothelial Nitric Oxide Synthase mRNA Expression in Human Platelets as Detected With Real-time Quantitative RT-PCR. Reviewed

    Shimasaki Y, Saito Y, Yoshimura M, Kamitani S, Miyamoto Y, Masuda I, Nakayama M, Mizuno Y, Ogawa H, Yasue H, and Nakao K.

    Clin Appl Thromb Hemost 雑誌   13   43 - 51   2007

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    Kind of work:Joint Work  

  • Enteropathogenic Escherichia coli, Shigella flexneri, and Listeria monocytogenes recruit a junctional protein, zonula occludens-1, to actin tails and pedestals. Reviewed

    Hanajima-Ozawa M, Matsuzawa T, Fukui A, Kamitani S, Ohnishi H, Abe A, Horiguchi Y, and Miyake M.

    Infect Immun 雑誌   75   565 - 57   2007

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    Kind of work:Joint Work  

  • Crystallization and preliminary crystallographic studies on the Pasteurella multocida toxin catalytic domain. Reviewed

    Miyazawa M, Kitadokoro K, Kamitani S, Shime H, and Horiguchi Y.

    Acta Crystallographica 雑誌   F62   906 - 908   2006

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    Kind of work:Joint Work  

  • Synthesis and properties of new macrocyclic compounds: Utilization of bond character of hypervalent sulfur in tetraazathiapentalene derivatives. Reviewed

    Matsumura N, Hirase R, Kamitani S, Okumura Y, and Mizuno K.

    J Heterocyclic Chem 雑誌   42   1175 - 1180   2005

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    Kind of work:Joint Work  

  • Large-scale Production of Functional Human Adrenomedullin: Expression, Cleavage, Amidation, and Purification. Reviewed

    Mitsuda Y, Takimoto A, Kamitani S, Kitamura K, Sakata T, and Mitsushima K.

    Protein Expr Purif 雑誌   25   448 - 455   2002

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    Kind of work:Joint Work  

  • Identification of a soluble-form phospholipase A2 receptor as a circulating endogenous inhibitor for secretory phospholipase A2 Reviewed

    Higashino K, Yokota Y, Ono T, Kamitani S, Arita H, and Hanasaki K.

    J Biol Chem 雑誌   277   13583 - 13588   2002

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    Kind of work:Joint Work  

  • Potent modification of low density lipoprotein by group X secretory phospholipase A2 is linked to macrophage foam cell formation. Reviewed

    Hanasaki K, Yamada K, Yamamoto S, Ishimoto Y, Saiga A, Ono T, Ikeda M, Notoya M, Kamitani S, and Arita H.

    J Biol Chem 雑誌   277   29116 - 29124   2002

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    Kind of work:Joint Work  

  • Glycosylation of human CRLR at Asn123 is required for ligand binding and signaling. Reviewed

    Kamitani S, and Sakata T.

    Biochim. Biophys. Acta. 雑誌   1539   131 - 139   2001

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    Kind of work:Joint Work  

  • Replication protein A1 reduces transcription of the endothelial nitric oxide synthase gene containing a -786T-->C mutation associated with coronary spastic angina. Reviewed

    Miyamoto Y, Saito Y, Nakayama M, Shimasaki Y, Yoshimura T, Yoshimura M, Harada M, Kajiyama N, Kishimoto I, Kuwahara K, Hino J, Ogawa E, Hamanaka I, Kamitani S, Takahashi N, Kawakami R, Kangawa K, Yasue H, and Nakao K.

    Hum Mol Genet 雑誌   9   2629 - 2637   2000

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    Kind of work:Joint Work  

  • Outside-in signaling of fibronectin stimulates cardiomyocyte hypertrophy in cultured neonatal rat ventricular myocytes. Reviewed

    Ogawa E, Saito Y, Harada M, Kamitani S, Kuwahara K, Miyamoto Y, Ishikawa M, Hamanaka I, Kajiyama N, Takahashi N, Nakagawa O, Masuda I, Kishimoto I, and Nakao K.

    J Mol Cell Cardiol 雑誌   32   765 - 776   2000

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    Kind of work:Joint Work  

  • Effects of cardiotrophin-1 on hemodynamics and endocrine function of the heart. Reviewed

    Hamanaka I, Saito Y, Nishikimi T, Magaribuchi T, Kamitani S, Kuwahara K, Ishikawa M, Miyamoto Y, Harada M, Ogawa E, Kajiyama N, Takahashi N, Izumi T, Shirakami G, Mori K, Inobe Y, Kishimoto I, Masuda I, Fukuda K, and Nakao K.

    Am J Physiol Heart Circ Physiol 雑誌   279   H388 - 396   2000

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    Kind of work:Joint Work  

  • Genetic risk factors for coronary artery spasm: significance of endothelial nitric oxide synthase gene T-786-->C and missense Glu298Asp variants. Reviewed

    Yoshimura M, Yasue H, Nakayama M, Shimasaki Y, Ogawa H, Kugiyama K, Saito Y, Miyamoto Y, Ogawa Y, Kaneshige T, Hiramatsu H, Yoshioka T, Kamitani S, Teraoka H, and Nakao K.

    J Investig Med 雑誌   48   367 - 374   2000

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  • Involvement of Cardiotrophin-1 in Cardiac Myocyte-Nonmyocyte Interactions During Hypertrophy of Rat Cardiac Myocytes In Vitro. Reviewed

    Kuwahara K, Saito Y, Harada M, Ishikawa M, Ogawa E, Miyamoto Y, Hamanaka I, Kamitani S, Kajiyama N, Takahashi N, Nakagawa O, Masuda I, and Nakao K.

    Circulation 雑誌   100   1116 - 1124   1999

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  • RAMP2/CRLR complex is a functional adrenomedullin receptor in human endothelial and vascular smooth muscle cells. Reviewed

    Kamitani S, Asakawa M, Shimekake Y, Kuwasako K, Nakahara K, and Sakata T.

    FEBS Lett. 雑誌   448   111 - 114   1999

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    Kind of work:Joint Work  

  • 内皮型一酸化窒素合成酵素遺伝子Glu298Asp変異と本態性高血圧症との連関研究.

    宮本 恵宏, 斎藤 能彦, 梶山 登, 上野 利恵, 神谷 重樹, 原田 昌樹, 石川 匡洋, 桑原 宏一郎, 小川 惠美子, 浜中 一郎, 高橋 伸基, 井野邊 義人, 桝田 出, 伊藤 裕, 吉政 孝明, 中尾 一和, 嶋崎 幸生, 中山 雅文, 吉村 道博, 泰江 弘文

    Therapeutic Research 雑誌   19   3072 - 3073   1998

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    Kind of work:Joint Work  

  • Endothelin-1 and cardiotrophin-1 induce brain natriuretic peptide gene expression by distinct transcriptional mechanisms. Reviewed

    Kuwahara K, Saito Y, Ogawa Y, Tamura N, Ishikawa M, Harada M, Ogawa E, Miyamoto Y, Hamanaka I, Kamitani S, Kajiyama N, Takahashi N, Nakagawa O, Masuda I, and Nakao K.

    J Cardiovasc Pharmacol 雑誌   31 (suppl. 1)   S354 - 356   1998

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    Kind of work:Joint Work  

  • Interaction of myocytes and nonmyocytes is necessary for mechanical stretch to induce ANP/BNP production in cardiocyte culture. Reviewed

    Harada M, Saito Y, Kuwahara K, Ogawa E, Ishikawa M, Nakagawa O, Miyamoto Y, Kamitani S, Hamanaka I, Kajiyama N, Takahashi N, Masuda I, Itoh H, and Nakao K.

    J Cardiovasc Pharmacol 雑誌   31 (suppl. 1)   S357 - 359   1998

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    Kind of work:Joint Work  

  • Endothelial Nitric Oxide Synthase Gene Is Positively Associated With Essential Hypertension. Reviewed

    Miyamoto Y, Saito Y, Kajiyama N, Yoshimura M, Shimasaki Y, Nakayama M, Kamitani S, Harada M, Ishikawa M, Kuwahara K, Ogawa E, Hamanaka I, Takahashi N, Kaneshige T, Teraoka H, Akamizu T, Azuma N, Yoshimasa Y, Yoshimasa T, Itoh H, Masuda I, Yasue H, and Nakao K.

    Hypertension 雑誌   32   3 - 8   1998

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    Kind of work:Joint Work  

  • ROLE OF CARDIAC NONMYOCYTES IN CYCLIC MECHANICAL STRETCH- INDUCED MYOCYTES HYPERTROPHY. Reviewed

    Harada M, Saito Y, Nakagawa O, Miyamoto Y, Ishikawa M, Kuwahara K, Ogawa E, Nakayama M, Kamitani S, Hamanaka I, Kajiyama N, Masuda I, Itoh H, and Nakao K.

    Heart and Vessels Suppl 雑誌   12   198 - 200   1997

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    Kind of work:Joint Work  

  • OPA(orphan peak analysis)法による遺伝子変異の検出

    神谷 重樹, 服部 正平

    検査と技術 雑誌   22   78 - 80   1994

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    Kind of work:Joint Work  

  • Four newly identified ornithine transcarbamylase (OTC) mutations (D126G, R129H, I172M and W332X) in Japanese male patients with early-onset OTC deficiency. Reviewed

    Matsuura T, Hoshide R, Kiwaki K, Komaki S, Koike E, Endo F, Oyanagi K, Suzuki Y, Kato I, Ishikawa K, Yoda H, Kamitani S, Sasaki Y, and Matsuda I.

    Hum Mutat 雑誌   3   402 - 406   1994

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    Kind of work:Joint Work  

  • The gene encoding a new mitogenic factor in a Streptococcus pyogenes strain is distributed only in group A streptococci. Reviewed

    Yutsudo T, Okumura K, Iwasaki M, Hara A, Kamitani S, Minamide W, Igarashi H, and Hinuma Y.

    Infect Immun 雑誌   62   4000 - 4004   1994

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    Kind of work:Joint Work  

  • In vitro catalysis of oxidative folding of disulfide-bonded proteins by Escherichia coli dsbA (ppfA) gene products. Reviewed

    Akiyama Y, Kamitani S, Kusukawa N and Ito K.

    J Biol Chem 雑誌   267   22440 - 22445   1992

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    Kind of work:Joint Work  

  • Identification and characterization of an Escherichia coli gene required for the formation of correctly folded alkaline phosphatase, a periplasmic enzyme. Reviewed

    Kamitani S, Akiyama Y, and Ito K.

    EMBO J 雑誌   11   57 - 62   1992

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    Kind of work:Joint Work  

  • Ring-Opening Reaction in Acidic Conditions of Tetraazapentalene Derivatives Containing a Hypervalent Sulfur. Reviewed

    Tomura M, Matsumura N, Mori O, Chikusa H, Kamitani S, and Inoue H.

    J Heterocyclic Chem 雑誌   27   2215 - 2217   1990

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    Kind of work:Joint Work  

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MISC

  • Evaluation of the effects of microplastic ingestion on condition in a NASH medaka model International journal

    岡部華子, 山本麻衣, 坂本丞, 坂本丞, 亀井保博, 神谷重樹, 神谷重樹

    日本分子生物学会年会プログラム・要旨集(Web)   46th   2023.12

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    Authorship:Corresponding author   Publishing type:Research paper, summary (national, other academic conference)   International / domestic magazine:Domestic journal  

    J-GLOBAL

  • Effects of Periodontal Pathogenic Bacterium Porphyromonas gingivalis (Pg) Administration on NASH Medaka Model Pathology International journal

    下本歩, 黒柳美和, 吉浦康寿, 坂本丞, 坂本丞, 亀井保博, 神谷重樹

    日本分子生物学会年会プログラム・要旨集(Web)   46th   2023.12

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    Authorship:Corresponding author   Publishing type:Research paper, summary (national, other academic conference)   International / domestic magazine:Domestic journal  

    J-GLOBAL

  • Investigation of vector-mediated administration of bacteria to Medaka International journal

    郷龍希, 彦坂悠衣, 亀井保博, 坂本丞, 坂本丞, 安齋賢, 神谷重樹

    日本分子生物学会年会プログラム・要旨集(Web)   46th   2023.12

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    J-GLOBAL

  • 最先端医療の今 抗肥満薬が黄色ブドウ球菌の病原因子を阻害するメカニズムを解明 International journal

    北所 健悟, 奥野 友紀子, 引間 孝明, 山本 雅貴, 神谷 重樹

    Medical Science Digest   49 ( 11 )   598 - 599   2023.10( ISSN:1347-4340

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    黄色ブドウ球菌(Staphylococcus aureus)リパーゼ(SAL)は重要な病原因子であり,感染症の治療標的となる可能性がある。抗肥満薬であるオルリスタットとSALとの複合体の立体構造を決定し,リパーゼとオルリスタットの結合様式を初めて明らかにした。構造はα/β hydrolase foldを持つ2量体であり,オルリスタットはSALと開環反応し,触媒残基Ser116に共有結合し,活性を阻害していた。ドラッグリポジショニングとして,オルリスタットの感染症治療薬への適応の可能性が示唆された。(著者抄録)

  • 最先端医療の今 抗肥満薬が黄色ブドウ球菌の病原因子を阻害するメカニズムを解明

    北所 健悟, 奥野 友紀子, 引間 孝明, 山本 雅貴, 神谷 重樹

    Medical Science Digest   49 ( 11 )   598 - 599   2023.10( ISSN:1347-4340

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    黄色ブドウ球菌(Staphylococcus aureus)リパーゼ(SAL)は重要な病原因子であり,感染症の治療標的となる可能性がある。抗肥満薬であるオルリスタットとSALとの複合体の立体構造を決定し,リパーゼとオルリスタットの結合様式を初めて明らかにした。構造はα/β hydrolase foldを持つ2量体であり,オルリスタットはSALと開環反応し,触媒残基Ser116に共有結合し,活性を阻害していた。ドラッグリポジショニングとして,オルリスタットの感染症治療薬への適応の可能性が示唆された。(著者抄録)

  • 歯周病原細菌Pg-LPS投与によるNASHモデルメダカ病態への影響 International journal

    上木 綾乃, 下本 歩, 伊藤 由佳子, 神谷 重樹

    日本栄養・食糧学会大会講演要旨集   77回   245 - 245   2023.05

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    Authorship:Corresponding author   Publishing type:Research paper, summary (national, other academic conference)   International / domestic magazine:Domestic journal  

  • アルテミアを利用した細菌のメダカへの投与の検討

    郷 龍希, 彦坂 悠衣, 坂本 丞, 亀井 保博, 神谷 重樹

    日本細菌学雑誌   78 ( 1 )   95 - 95   2023.02( ISSN:0021-4930 ( eISSN:1882-4110

  • NASHメダカモデルにおけるマイクロプラスチック摂取が腸内細菌叢に及ぼす影響の評価 International journal

    岡部 華子, 山本 麻衣, 坂本 丞, 亀井 保博, 神谷 重樹

    日本細菌学雑誌   78 ( 1 )   73 - 73   2023.02( ISSN:0021-4930 ( eISSN:1882-4110

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  • 骨粗しょう症モデルメダカの確立

    彦坂 悠衣, 安齋 賢, 浅尾 桃子, 青山 智絵, 斎田 美佐子, 坂本 丞, 亀井 保博, 神谷 重樹

    日本栄養・食糧学会大会講演要旨集   76回   296 - 296   2022.05

  • 哺乳類以外のモデル生物を用いた栄養学研究の展開 メダカを用いた生活習慣病研究

    神谷 重樹

    日本栄養・食糧学会大会講演要旨集   76回   156 - 156   2022.05

  • 羽曳野産イチジク葉抽出物に含まれる歯周病原細菌に対する増殖阻害活性成分の解析

    藤井 智也, 三宅 彩優奈, 宮下 千穂, 倉橋 雪乃, 北田 康裕, 高井 雄一郎, 神谷 重樹

    日本栄養・食糧学会大会講演要旨集   74回   264 - 264   2020.04

  • 女子大学生スポーツ選手の口腔細菌叢と生活背景に関する検討

    矢澤 彩香, 古宮 綾乃, 和田 敏美, 徳本 勇人, 尾形 善之, 小川 由紀子, 神谷 重樹

    日本スポーツ栄養研究誌   13   177 - 177   2020.01( ISSN:2188-8922

  • 抗酸菌症治療薬を目指した標的蛋白質の発現と精製

    大原 由貴子, 小林 悠, 尾関 百合子, 西山 晃史, 立石 善隆, 奥田 修二郎, 神谷 重樹, 北所 健悟, 松本 壮吉

    日本細菌学雑誌   75 ( 1 )   74 - 74   2020.01( ISSN:0021-4930 ( eISSN:1882-4110

  • 抗菌活性を持つプルニンラウリン酸エステルによる歯周病抑制効果の評価

    和田 衣里香, 伊藤 千陽, 篠原 舞, 前谷 実希, 矢澤 彩香, 阪本 龍司, 安木 真世, 三宅 眞実, 神谷 重樹

    日本細菌学雑誌   75 ( 1 )   151 - 151   2020.01( ISSN:0021-4930 ( eISSN:1882-4110

  • Evaluation of antibacterial prunin lauroyl ester on mouse model of experimental periodontitis

    和田衣里香, 伊藤千陽, 篠原舞, 前谷実希, 矢澤彩香, 阪本龍司, 安木真世, 三宅眞実, 神谷重樹

    日本細菌学雑誌(Web)   75 ( 1 )   2020( ISSN:1882-4110

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  • 噴門形成術後のダンピング症状に対してミルクの粘度調整が有効であったCHARGE症候群の一症例

    花井 美夢, 西本 裕紀子, 麻原 明美, 加嶋 倫子, 伊藤 真緒, 黒川 通典, 神谷 重樹, 岡田 洋介, 錦戸 知喜, 大沼 真輔, 惠谷 ゆり, 位田 忍

    New Diet Therapy   35 ( 3 )   3 - 9   2019.12( ISSN:0910-7258

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    症例は1歳4ヵ月男児で、CHARGE症候群に罹患しており、嚥下障害、食道裂孔ヘルニア、胃食道逆流のため、生後4ヵ月時に胃瘻造設、噴門形成術が施行された。1歳3ヵ月時に呼吸器感染により当院に入院となり、胃瘻からミルク(濃度14.7%)200mL×5回を各1時間で注入して栄養管理を行っていた。しかし、感染改善後も活気が乏しく、ミルク注入30分後に簡易血糖測定器を用いて血糖測定を行ったところ、2時間後に血糖値118mg/dLとなり、後期ダンピング症候群と診断された。治療方針として、増粘剤を用いてミルクの粘度調整を行い、血糖コントロールすることとした。ミルク200mLに対してトロミ調整食品「トロミスマイル」1.2g(容量比0.6%)を用いて粘度調整を行い、ポンプを用いて1時間で注入したところ、30分後の血糖値は200mg/dLとなった。さらにトロミスマイル1.4g(容量比0.7%)に増量し、その後5日間の各注入30分後の血糖値は中央値108mg/dLで安定して経過した。

  • 構造生物学から観た寄生適応の分子戦略 黄色ブドウ球菌由来毒素リパーゼと阻害剤のドラッグデザイン

    北所 健悟, 田中 睦美, 古澤 昌大, 神谷 重樹

    日本生化学会大会プログラム・講演要旨集   92回   [2S06m - 06]   2019.09

  • 「なにわの伝統野菜」'田辺'および'守口'ダイコンの糖含量および物理的特性

    山下 絵美, 高井 雄一郎, 北田 康祐, 山崎 基嘉, 神谷 重樹

    日本栄養・食糧学会大会講演要旨集   73回   263 - 263   2019.04

  • 羽曳野産イチジク葉抽出物の歯周病原細菌に対する抗菌活性の検討

    三宅 彩優奈, 宮下 千穂, 倉橋 雪乃, 藤井 智也, 北田 康祐, 高井 雄一郎, 神谷 重樹

    日本栄養・食糧学会大会講演要旨集   73回   270 - 270   2019.04

  • 女子大学生スポーツ選手の口腔細菌叢と口腔ケアに関する検討

    矢澤 彩香, 古宮 綾乃, 和田 敏美, 渡邊 完児, 松木 優也, 徳本 勇人, 尾形 善之, 小川 由紀子, 神谷 重樹

    日本栄養・食糧学会大会講演要旨集   73回   304 - 304   2019.04

  • プルニンラウリン酸エステルの歯周病抑制効果の検討

    和田 衣里香, 伊藤 千陽, 篠原 舞, 前谷 実希, 矢澤 彩香, 安木 真世, 三宅 眞実, 阪本 龍司, 神谷 重樹

    日本栄養・食糧学会大会講演要旨集   73回   271 - 271   2019.04

  • ‘田辺’ダイコンの根部の物性および糖含量の特性

    山下絵美, 山下絵美, 高井雄一郎, 北田康祐, 山崎基嘉, 神谷重樹

    新近畿中国四国農業研究(Web)   ( 2 )   1‐12 (WEB ONLY)   2019.03( ISSN:2433-796X

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  • 黄色ブドウ球菌が分泌するリパーゼのX線結晶構造解析

    田中 睦美, 神谷 重樹, 北所 健悟

    日本細菌学雑誌   74 ( 1 )   140 - 140   2019.03( ISSN:0021-4930

  • 女子大学生スポーツ選手の歯周病原細菌感染状況に関する検討

    矢澤 彩香, 和田 敏美, 渡邊 完児, 松木 優也, 徳本 勇人, 尾形 善之, 神谷 重樹

    日本スポーツ栄養研究誌   12   122 - 122   2019.01( ISSN:2188-8922

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Presentations

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Industrial Property Rights

  • 遺伝子の変異同定法

    松田一郎,遠藤文夫,信国好俊,三渕浩,松浦稔展,浅香純一郎,兼重俊彦,矢追毅,神谷重樹

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    property_type:Patent 

Outline of collaborative research (seeds)

  • メダカを用いた生活習慣病モデル動物の作製と応用

  • 歯周病原細菌に対する抗菌作用を持つ食品成分の探索

  • 食品などの口腔細菌叢に与える影響の解析

Outline of education staff

  • 生活科学部、生活科学研究科にて、食品衛生学、感染防御学を担当。

Charge of on-campus class subject

  • Social Health Science I

    2021    

  • Special Topics in Foods and Human Wellness

    2021    

  • Advanced Seminar in Nutrition Sciences C

    2021    

  • Advanced Seminar in Nutrition Sciences B

    2021    

  • Advanced Seminar in Nutrition Sciences A

    2021    

  • Food Sanitation (Laboratory)

    2021   Practical Training  

  • Basic genomics

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Job title

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    School of Human Life and Ecology Department of Nutrition 

    学科長  2024.04