Updated on 2024/02/20

写真a

 
Fujieda Nobutaka
 
Organization
Graduate School of Agriculture Department of Applied Biological Chemistry Professor
School of Agriculture Department of Applied Biological Chemistry
Title
Professor
Affiliation
Institute of Agriculture
Contact information
メールアドレス
Affiliation campus
Nakamozu Campus

Position

  • Graduate School of Agriculture Department of Applied Biological Chemistry 

    Professor  2022.04 - Now

  • School of Agriculture Department of Applied Biological Chemistry 

    Professor  2022.04 - Now

Degree

  • 博士(農学) ( Kyoto University )

Research Areas

  • Nanotechnology/Materials / Analytical chemistry

  • Nanotechnology/Materials / Inorganic/coordination chemistry

  • Nanotechnology/Materials / Chemistry and chemical methodology of biomolecules

  • Life Science / Functional biochemistry

  • Nanotechnology/Materials / Bio chemistry

  • Nanotechnology/Materials / Chemistry and chemical methodology of biomolecules

  • Life Science / Bioorganic chemistry

  • Nanotechnology/Materials / Bio chemistry

  • Nanotechnology/Materials / Inorganic/coordination chemistry

▼display all

Research Interests

  • Bioinorganic Chemistry

  • Artificial Metalloenzyme

  • Biomolecular Technology

  • Biomimetics

  • Xenobiology

  • Chemomimetics

  • Artzyme

Professional Memberships

  • The Society of Biological Inorganic Chemistry

    2021

  • 日本蛋白質科学会

    2020

  • JAPAN SOCIETY OF COORDINATION CHEMISTRY

    2010

  • THE CHEMICAL SOCIETY OF JAPAN

    2006

  • JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY

    2000

Awards

  • Asian Core Program Lectureship Award (China)

    2022.07   ICCEOCA-15  

  • 2020年度学長顕彰

    2020.08   大阪府立大学  

  • 2019年度学長顕彰

    藤枝 伸宇

    2019.08   大阪府立大学  

  • 長瀬研究振興賞

    藤枝 伸宇

    2019.04   長瀬科学技術振興財団  

  • 日本農芸化学奨励賞

    藤枝 伸宇

    2019.03   日本農芸化学会  

  • 第99回春季年会 第33回 若い世代の特別講演証

    藤枝 伸宇

    2019.03   日本化学会  

▼display all

Job Career (off-campus)

  • Osaka Metropolitan University   Graduate School of Agriculture

    2022.04 - Now

  • Osaka Prefecture University   Graduate School of Life and Environmental Sciences

    2021.04 - 2022.03

  • Osaka Prefecture University   Graduate School of Life and Environmental Sciences

    2017.04 - 2021.03

  • 科学技術振興機構JSTさきがけ研究員

    2016.10 - Now

  • JST   PRESTO   Researcher

    2016.10 - 2020.03

  • 大阪大学蛋白質研究所共同研究員

    2013.04 - Now

  • Osaka University   Institute for Protein Research   Research Fellow

    2013.04 - Now

  • バーゼル大学化学科(スイス)客員教授

    2013.04 - 2014.03

  • 大阪大学大学院工学研究科生命先端工学専攻・助教

    2009.04 - 2017.03

  • 京都大学エネルギー理工学研究所・附属エネルギー複合機構研究センター共同研究者

    2007.04 - 2009.03

▼display all

Education

  • Kyoto University   Graduate School, Division of Agriculture   The second semester of doctoral program   Graduated/Completed

    2003.04 - 2006.03

  • Kyoto University   Graduate School, Division of Agriculture   The first semester of doctoral program   Graduated/Completed

    2001.04 - 2003.03

  • Kyoto University   Faculty of Agriculture   Bachelor's Course   Graduated/Completed

    1997.04 - 2001.03

Papers

  • Asymmetric Michael addition catalysed by copper–amyloid complexes Reviewed

    Nobutaka Fujieda, Atsushi Tonomura, Tomofumi Mochizuki, Shinobu Itoh

    RSC Advances   14 ( 1 )   206 - 210   2024.01

     More details

    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)  

    Amyloid proteins displaying a copper histidine-brace offer designable scaffolds for efficient and asymmetric chemical reactions.

    DOI: 10.1039/d3ra07900g

  • An Artificial Metallolyase with Pliable 2-His-1-Carboxylate Facial Triad for Stereoselective Michael Addition Reviewed

    Ryusei Matsumoto, Saho Yoshioka, Miho Yuasa, Yoshitsugu Morita, Genji Kurisu, Nobutaka Fujieda

    Chemical Science   2023.03( ISSN:2041-6520

     More details

    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    We repurposed the metal-binding site of a cupin superfamily protein into the 2-his-1-carboxylate facial triad, which is one of the common motifs in natural non-heme enzymes, to construct artificial metalloenzymes...

    DOI: 10.1039/d2sc06809e

  • Electrochemical Consideration of Electrostatic Interaction of Charged Molecules with Partially Overlapped Electric Field: Zwitterions and Proteins Reviewed

    Yu SUGIMOTO, Nobutaka FUJIEDA, Kenji KANO

    Electrochemistry   2021.03( ISSN:1344-3542

     More details

    Publishing type:Research paper (scientific journal)  

    DOI: 10.5796/electrochemistry.21-00030

  • Biochemical Characterization of a Group-5 Soluble Diiron Monooxygenase Hydroxylase and Related Chaperonin-like Component

    Chihiro Inoue, Yoshitaka Abe, Nobutaka Fujieda

    2020.10

     More details

    Authorship:Last author, Corresponding author  

    <p>Recently, the functional expression of group-5 hydroxylase component (MimA and MimC) in <i>Escherichia coli </i>along with its related chaperonin-like component (MimG) was reported by Furuya and Kino. In this study, we report the purification via a heterologous expression system and the biochemical characterization of MimAC, the complex of MimA and MimC and MimG to understand their exact roles. MimAC and MimG were fused with His-tags and purified using affinity chromatography in a homogenous state on SDS-PAGE. Blue native PAGE demonstrated that the quaternary structure of MimG was almost identical to that of chaperonin GroEL, indicating that its function was also similar to GroEL. Size-exclusion chromatography and ICP-AES analysis demonstrated that MimAC was assembled in the dimer of two sort of subunits and exhibited two iron atoms and at least one zinc atom per two subunits. This result indicated that MimAC possessed a dinuclear iron center, similar to other soluble diiron monooxygenase hydroxylases.</p>

    DOI: 10.26434/chemrxiv.13110602

  • Copper–Oxygen Dynamics in the Tyrosinase Mechanism Reviewed

    Nobutaka Fujieda, Kyohei Umakoshi, Yuta Ochi, Yosuke Nishikawa, Sachiko Yanagisawa, Minoru Kubo, Genji Kurisu, Shinobu Itoh

    Angewandte Chemie International Edition   59 ( 32 )   13385 - 13390   2020.08( ISSN:1433-7851

     More details

    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/anie.202004733

    Other URL: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/anie.202004733

  • Copper–Oxygen Dynamics in the Tyrosinase Mechanism

    Nobutaka Fujieda, Kyohei Umakoshi, Yuta Ochi, Yosuke Nishikawa, Sachiko Yanagisawa, Minoru Kubo, Genji Kurisu, Shinobu Itoh

    Angewandte Chemie   132 ( 32 )   13487 - 13492   2020.05( ISSN:0044-8249

     More details

    Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/ange.202004733

    Other URL: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/ange.202004733

  • Cupin Variants as a Macromolecular Ligand Library for Stereoselective Michael Addition of Nitroalkanes Reviewed

    Nobutaka Fujieda, Haruna Ichihashi, Miho Yuasa, Yosuke Nishikawa, Genji Kurisu, Shinobu Itoh

    Angewandte Chemie International Edition   59 ( 20 )   7717 - 7720   2020.05( ISSN:1433-7851

     More details

    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/anie.202000129

    Other URL: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/anie.202000129

  • His-Cys and Trp-Cys Cross-links Generated by Post-translational Chemical Modification Nobutaka Fujieda Reviewed

    Nobutaka Fujieda

    Biosci. Biotech. Biochem. 雑誌   84 ( 3 )   2020.03

     More details

    Kind of work:Single Work  

  • Tyrosinases in Organic Chemistry: a Versatile Tool for the α-Arylation of β-Dicarbonyl Compounds Reviewed

    Roxanne Krug, Dennis Schröder, Jan Gebauer, Sanel Suljić, Yuma Morimoto, Nobutaka Fujieda, Shinobu Itoh, and Jörg Pietruszkaa

    Euro. J. Org. Chem.   2018.07

     More details

    Kind of work:Joint Work  

  • Did catalytic amyloid play the significant role in chemical evolution? Reviewed

    Nobutaka Fujieda

    Viva Origino   2018.04

     More details

    Kind of work:Single Work  

  • 銅含有モノオキシゲナーゼによる酸素分子の活性化機構 Reviewed

    伊東 忍・藤枝伸宇

    生化学   2018.03

     More details

    Kind of work:Joint Work  

  • Geometric Effects on O–O bond Scission of Copper(II)-alkylperoxide Reviewed

    Tsukasa Abe, Yuma Morimoto, Kaoru Mieda, Hideki Sugimoto, Nobutaka Fujieda, Takashi Ogura, and Shinobu Itoh

    J. Inorg. Biochem.   2017.12

  • Tetrahedral Copper(II) Complexes with a Labile Coordination Site Supported by a tris-Tetramethylguanidinato Ligand

    Ikuma Shimizu, Yuma Morimoto, Dieter Faltermeier, Marion Kerscher, Sayantan Paria, Tsukasa Abe, Hideki Sugimoto, Nobutaka Fujieda, Kaori Asano, Takeyuki Suzuki, Peter Comba, and Shinobu Itoh

    Inorg. Chem.   2017.10

  • Generation and Characterisation of a Stable Nickel(II)-Aminoxyl Radical Complex Reviewed

    Arnaud Parrot, Yuma Morimoto, Sayantan Paria, Hideki Sugimoto, Nobutaka Fujieda, and Shinobu Itoh

    Dalton Trans.   2017.09

     More details

    Kind of work:Joint Work  

  • A Well-Defined Osmium-Cupin Complex: Hyperstable Artificial Osmium Peroxygenase Reviewed

    Nobutaka Fujieda, Takumi Nakano, Yuki Taniguchi, Haruna Ichihashi, Hideki Sugimoto, Yuma Morimoto, Yosuke Nishikawa, Genji Kurisu, and Shinobu Itoh

    J. Am. Chem. Soc. 雑誌   139 ( 14 )   2017.04

     More details

    Kind of work:Joint Work  

  • Controlling dicopper protein functions Reviewed

    Nobutaka Fujieda and Shinobu Itoh

    Bull. Chem. Soc. Jpn.   2016.03

     More details

    Kind of work:Joint Work  

  • Enzyme Repurposing of a Hydrolase as an Emergent Peroxidase upon Metal Binding Reviewed

    Nobutaka Fujieda, Jonas Schätti, Edward Stuttfeld, Kei Ohkubo, Timm Maier, and Thomas R. Ward

    Chem. Sci.   2015.11

     More details

    Kind of work:Joint Work  

  • Generation, Characterization, and Reactivity of a CuII−Alkylperoxide/Anilino Radical Complex: Insight into the O−O Bond Cleavage Mechanism Reviewed

    Sayantan Paria, Takehiro Ohta, Yuma Morimoto, Takashi Ogura, Hideki Sugimoto, Nobutaka Fujieda, Kei Goto, Kaori Asano, Takeyuki Suzuki, and Shinobu Itoh

    J. Am. Chem. Soc.   2015.09

     More details

    Kind of work:Joint Work  

  • Direct Hydroxylation of Benzene to Phenol Using Hydrogen Peroxide Catalyzed by Nickel Complexes Supported by Pyridylalkylamine Ligands Reviewed

    Yuma Morimoto, Shuji Bunno, Nobutaka Fujieda, Hideki Sugimoto, and Shinobu Itoh

    J. Am. Chem. Soc.   2015.07

     More details

    Kind of work:Joint Work  

  • Redox behavior of novel nickel and palladium complexes supported by trianionic non-innocent ligand containing beta-diketiminate and phenol groups Reviewed

    Yuma Morimoto, June Takaichi, Shinichi Hanada, Kei Ohkubo, Hideki Sugimoto, Nobutaka Fujieda, Shunichi Fukuzumi and Shinobu Itoh

    J. Porphyrins Phthalocyanines   2015.06

     More details

    Kind of work:Joint Work  

  • Geometric Control of Nuclearity in Copper(I)/Dioxygen Chemistry Reviewed

    Tsukasa Abe, Yuma Morimoto, Tetsuro Tano, Kaoru Mieda, Hideki Sugimoto, Nobutaka Fujieda, Takashi Ogura, and Shinobu Itoh

    Inorg. Chem.   2014.12

     More details

    Kind of work:Joint Work  

  • Redox Chemistry of Nickel(II)-Complexes Supported by a Series of Non-innocent beta-Diketiminate Ligands Reviewed

    June Takaichi, Yuma Morimoto, Kei Ohkubo, Chizu Shimokawa, Takayuki Hojo, Seiji Mori, Haruyasu Asahara, Hideki Sugimoto, Nobutaka Fujieda, Nagatoshi Nishiwaki, Shunichi Fukuzumi, and Shinobu Itoh

    Inorg. Chem.   2014.10

     More details

    Kind of work:Joint Work  

  • 二核銅タンパク質の機能解明と触媒への応用 Reviewed

    伊東 忍・藤枝伸宇

    触媒年鑑   2014.08

     More details

    Kind of work:Joint Work  

  • Redox Properties of Mononuclear Copper(II)-Superoxide Complex Reviewed

    Tetsuro Tano, Yuri Okubo, Atsushi Kunishita, Minoru Kubo, Hideki Sugimoto, Nobutaka Fujieda, Takashi Ogura, and Shinobu Itoh

    Inorg. Chem.   2013.12

     More details

    Kind of work:Joint Work  

  • Crystal Structures of Copper-Depleted and Copper-Bound Fungal Pro-Tyrosinase: Insights Into Endogenous Cysteine-Dependent Copper Incorporation Reviewed

    Nobutaka Fujieda, Shintaro Yabuta, Takuya Ikeda, Takuji Oyama, Norifumi Muraki, Genji Kurisu, and Shinobu Itoh

    J. Biol. Chem.   2013.09

     More details

    Kind of work:Joint Work  

  • Copper Complexes of the Non-innocent beta-Diketiminate Ligand Containing Phenol Groups Reviewed

    June Takaichi, Kei Ohkubo, Hideki Sugimoto, Motohiro Nakano, Daisuke Usa, Hiroaki Maekawa, Nobutaka Fujieda, Nagatoshi Nishiwaki, Shu Seki, Shunichi Fukuzumi, and Shinobu Itoh

    Dalton Trans.   2013.07

     More details

    Kind of work:Joint Work  

  • Activation Mechanism of MelB Tyrosinase from Aspergillus oryzae by Acidic Treatment Reviewed

    Nobutaka Fujieda, Michiaki Murata, Shintaro Yabuta, Takuya Ikeda, Chizu Shimokawa, Yukihiro Nakamura, Yoji Hata, Shinobu Itoh

    J. Biol. Inorg. Chem.   2013.05

     More details

    Kind of work:Joint Work  

  • Active Site Models for the CuB Site of Peptidylclycine alpha-Hydroxylating Monooxygenase and Dopamine alpha-Monooxygenase Reviewed

    Atsushi Kunishita, Mehmed Z. Ertem, Yuri Okubo, Tetsuro Tano, Hideki Sugimoto, Kei Ohkubo, Nobutaka Fujieda, Shuichi Fukuzumi, Christopher J. Cramer, and Shinobu Itoh

    Inorg. Chem.   2012.12

     More details

    Kind of work:Joint Work  

  • Heterolytic Alkylhydroperoxide O–O Bond Cleavage by Copper(I) Complexes Reviewed

    Tetsuro Tano, Hideki Sugimoto, Nobutaka Fujieda, and Shinobu Itoh

    Eur. J. Inorg. Chem.   2012.10

     More details

    Kind of work:Joint Work  

  • Copper(I)-Dioxygen Reactivity in a Sterically Demanding Tripodal Tetradentate tren Ligand. Formation and Reactivity of a Mononuclear Copper(II) End-on Superoxo Complex Reviewed

    Yuki Kobayashi, Kei Okubo, Takashi Nomura, Minoru Kubo, Nobutaka Fujieda, Hideki Sugimoto, Shunichi Fukuzumi, Kei Goto, Takashi Ogura, and Shinobu Itoh

    Eur. J. Inorg. Chem.   2012.08

     More details

    Kind of work:Joint Work  

  • Artificial Dicopper Oxidase: Rational Reprogramming of Bacterial Metallo-beta-lactamase into a Catechol Oxidase Reviewed

    Nobutaka Fujieda, Atsushi Hasegawa, Kenichi Ishihama, and Shinobu Itoh

    Chem. Asian J.   2012.06

     More details

    Kind of work:Joint Work  

  • Multifunctions of MelB, a Fungal Tyrosinase from Aspergillus oryzae Reviewed

    Nobutaka Fujieda, Michiaki Murata, Shintaro Yabuta, Takuya Ikeda, Chizu Shimokawa, Yukihiro Nakamura, Yoji Hata, and Shinobu Itoh

    ChemBioChem.   2012.05

     More details

    Kind of work:Joint Work  

  • Reactivity of Copper(II)-alkylperoxo Complexes Reviewed

    Tetsuro Tano, Mehmed Z. Ertem, Satoru Yamaguchi, Atsushi Kunishita, Hideki Sugimoto, Nobutaka Fujieda, Takashi Ogura, Christopher J. Cramer*, and Shinobu Itoh

    Dalton Trans.   2011.12

     More details

    Kind of work:Joint Work  

  • Post-translational His-Cys Cross Linkage Formation in Tyrosinase Induced by Copper(II)-Peroxo Species Reviewed

    Nobutaka Fujieda, Takuya Ikeda, Michiaki Murata, Sachiko Yanagisawa, Shigetoshi Aono, Kei Ohkubo, Satoshi Nagao, Takashi Ogura, Shun Hirota, Shunichi Fukuzumi, Yukihiro Nakamura, Yoji Hata, and Shinobu Itoh

    J. Am. Chem. Soc.   2011.10

     More details

    Kind of work:Joint Work  

  • Reactions of Copper(II)-Phenol Systems with O2. Models for TPQ Biosynthesis in Copper Amine Oxidases Reviewed

    Kae Tabuchi, Mehmed Z. Ertem, Hideki Sugimoto, Atsushi Kunishita, Tetsuro Tano, Nobutaka Fujieda, Christopher J. Cramer, and Shinobu Itoh

    Inorg. Chem.   2011.05

     More details

    Kind of work:Joint Work  

  • Five Monomeric Hemocyanin Subunits from Portunus trituberculatus: Purification, Spectroscopic Characterization, and Quantitative Evaluation of Phenol Monooxygenase Activity Reviewed

    Nobutaka Fujieda, Aki Yakiyama, and Shinobu Itoh

    Biochem. Biophys. Acta   2010.10

     More details

    Kind of work:Joint Work  

  • 銅タンパク質の水酸化触媒としての応用 Reviewed

    藤枝伸宇・伊東 忍

    化学工業   2010.09

     More details

    Kind of work:Joint Work  

  • Oxygenation Chemistry at Mononuclear Copper(II)-Hydroquinone Sysetm with O2 Reviewed

    Kae Tabuchi, Hideki Sugimoto, Atsushi Kunishita, Nobutaka Fujieda, and Shinobu Itoh

    Inorg. Chem.   2010.07

     More details

    Kind of work:Joint Work  

  • Catalytic Oxygenation of Phenols by Arthropod Hemocyanin, an Oxygen Carrier Protein, from Portunus trituberculatus Reviewed

    Nobutaka Fujieda, Aki Yakiyama, and Shinobu Itoh

    Dalton Trans.   2010.05

     More details

    Kind of work:Joint Work  

  • Site-directed mutation at residues near the catalytic site of histamine dehydrogenase from Nocardioides simplex and its effects on substrate inhibition Reviewed

    Maiko Tsutsumi, Noriaki Tsuse, Nobutaka Fujieda, and Kenji Kano

    J. Biochem.   2010.03

     More details

    Kind of work:Joint Work  

  • A single circularly permuted GFP sensor for inositol-1,3,4,5-tetrakisphosphate based on a split PH domain Reviewed

    Reiko Sakaguchi, Takashi Endoh, Seigo Yamamoto, Kazuki Tainaka, Kenji Sugimoto, Nobutaka Fujieda, Shigeki Kiyonaka, Yasuo Mori, and Takashi Morii

    Bioorg. Med. Chem.   2009.10

     More details

    Kind of work:Joint Work  

  • The Silent Form of Quinohemoprotein Amine Dehydrogenase from Paracoccus denitrificans Reviewed

    Nobutaka Fujieda, Megumi Mori, Tokuji Ikeda, and Kenji Kano

    Biosci. Biotech. Biochem.   2008.12

     More details

    Kind of work:Joint Work  

  • Bioelectrochemical Determination at Histamine Dehydrogenase-based Electrodes Reviewed

    Ryhei Yamada. Nobutaka Fujieda, Maiko Tsutsumi, Seiya Tsujimura, Osamu Shirai and Kenji Kano

    Electrochemistry   2008.10

     More details

    Kind of work:Joint Work  

  • Context-dependent Fluorescence Detection of a Phosphorylated Tyrosine Residue by a Ribonucleopeptide Reviewed

    Tetsuya Hasegawa, Masaki Hagihara, Masatora Fukuda, Shun Nakano, Nobutaka Fujieda and Takashi Morii

    J. Am. Chem. Soc.   2008.07

     More details

    Kind of work:Joint Work  

  • Thermodynamic Redox Properties Governing the Half-Reduction Characteristics of Histamine Dehydrogenase from Nocardioides simplex Reviewed

    Maiko Tsutsumi, Nobutaka Fujieda, Seiya Tsujimura, Osamu Shirai and Kenji Kano

    Biosci. Biotech. Biochem.   2008.05

     More details

    Kind of work:Joint Work  

  • Model Studies of 6,7-Indolequinone Cofactors of Quinohemoprotein Amine Dehydrogenases Reviewed

    Murakami, Y., Yoshimoto, Nobutaka Fujieda, Kei Ohkubo, Kenji Kano, Shunichi Fukuzumi and Shinobu Itoh

    J. Org. Chem.   2007.10

     More details

    Kind of work:Joint Work  

  • Production of Completely Flavinylated Histamine Dehydrogenase, Unique Covalently Bound Flavin and Iron-Sulfur Cluster Containing Enzyme, of Nocardioides simplex in Escherichia coli and Its Properties Reviewed

    Nobutaka Fujieda, Noriaki Tsuse, Astuko Satoh, Tokuji Ikeda, and Kenji Kano

    Biosci. Biotech. Biochem.   2005.10

     More details

    Kind of work:Joint Work  

  • Mediated Spectroelectrochemical Titration of Proteins for Redox Potential Measurements by a Separator-less One-compartment Bulk Electrolysis Method Reviewed

    Seiya Tsujimura, Atsushi Kuriyama, Nobutaka Fujieda, Kenji Kano, and Tokuji Ikeda

    Anal. Biochem.   2005.06

     More details

    Kind of work:Joint Work  

  • 6-S-Cysteinyl Flavin Mononucleotide-containing Histamine Dehydrogenase from Nocardioides simplex: Molecular Cloning, Sequencing, Over-Expression and Characterization of Redox Centers of Enzyme Reviewed

    Nobutaka Fujieda, Astuko Satoh, Noriaki Tsuse, Kenji Kano, and Tokuji Ikeda

    Biochemistry   2004.10

     More details

    Kind of work:Joint Work  

  • Separator-less One-compartment Bulk Electrolysis with a Small Auxiliary Electrode and Its Application to Spectroelectrochemistry Reviewed

    Atsushi Kuriyama, Moriaki Arasaki, Nobutaka Fujieda, Seiya Tsujimura, Kenji Kano, and Tokuji Ikeda

    Electrochemistry   2004.06

     More details

    Kind of work:Joint Work  

  • DsbB Elicits a Red-shift of Bound Ubiquinone during the Catalysis of DsbA Oxidation Reviewed

    Kenji Inaba, Yoh-hei Takahashi, Nobutaka Fujieda, Kenji Kano, Hideto Miyoshi, and Koreaki Ito

    J. Biol. Chem.   2004.04

     More details

    Kind of work:Joint Work  

  • Redox Properties of Quinohemoprotein Amine Dehydrogenase from Paracoccus denitrificans Reviewed

    Nobutaka Fujieda, Megumi Mori, Kenji Kano, and Tokuji Ikeda

    Biochim. Biophys. Acta   2003.10

     More details

    Kind of work:Joint Work  

  • Spectroelectrochemical Evaluation of Redox Potentials of Cysteine Tryptophylquinone and Two Hemes c in Quinohemoprotein Amine Dehydrogenase from Paracoccus denitrificans Reviewed

    Nobutaka Fujieda, Megumi Mori, Kenji Kano, and Tokuji Ikeda

    Biochemistry   2002.12

     More details

    Kind of work:Joint Work  

▼display all

Books and Other Publications

  • Encyclopedia of Catalysts and Catalysis

    ( Role: Contributor ,  8-24)

    2023.02  ( ISBN:9784254252743

     More details

    Total pages:xiv, 530p  

  • 銅酵素の化学(生命金属ダイナミクス)

    藤枝伸宇、伊東 忍( Role: Joint author)

    エヌ・ティー・エス   2021.01 

  • 生命金属ダイナミクス ~生体内における金属の挙動と制御~

    藤枝伸宇, 伊東 忍( Role: Contributor ,  第4章第5節 銅酸素の化学)

    エヌ・ティー・エス  2021  ( ISBN:9784860437060

  • 非ヘム人工金属酵素の機能創製

    藤枝伸宇

    化学と工業  2019.12 

  • 高立体選択的 C-C 結合反応を触媒する人工金属酵素の創製

    藤枝伸宇

    日本応用酵素協会誌  2019.04 

  • Assembly of Dinuclear Copper Center in Tyrosinase and Hemocyanins [Metalloprotein Active Site Assembly (Handbook of Metalloproteins to the Encyclopedia of Inorganic and Bioinorganic Chemistry)]

    Nobutaka Fujieda and Shinobu Itoh( Role: Joint author)

    John Wiley & Sons, Ltd  2017.09 

  • Fungal Pro-tyrosinase (Handbook of Metalloproteins to the Encyclopedia of Inorganic and Bioinorganic Chemistry)

    Nobutaka Fujieda and Shinobu Itoh( Role: Joint author)

    John Wiley & Sons, Ltd  2015.06 

▼display all

MISC

  • 立体選択的反応を触媒する人工金属酵素のデザイン Invited

    藤枝伸宇, 松本隆聖

    バイオサイエンスとインダストリー   81 ( 6 )   520 - 521   2023.06

     More details

    Authorship:Lead author, Corresponding author  

  • チロシナーゼ反応機構における銅活性酸素種の動的挙動 Reviewed

    藤枝伸宇・伊東 忍

    生化学   93 ( 3 )   2021.08

     More details

  • チロシナーゼ反応機構における銅活性酸素種の動的挙動

    藤枝 伸宇, 伊東 忍

    生化学   93 ( 4 )   521 - 525   2021.08( ISSN:0037-1017

     More details

    近年、非ヘム金属酵素の一つである銅蛋白質チロシナーゼについて、銅中心の動的挙動が多数報告されている。筆者らは、麹菌由来チロシナーゼの高分解能X線結晶構造解析を行い、チロシナーゼのモノオキシゲナーゼ反応における分子機構の解明に取り組んできた。これまでに提唱されたチロシナーゼ反応機構の変遷、銅活性酸素種の挙動に関する最新の反応機構について概説した。

  • チロシナーゼ反応機構における銅活性酸素種の動的挙動 Invited

    藤枝伸宇, 伊東 忍

    生化学   93 ( 3 )   521 - 525   2021

     More details

    Authorship:Lead author   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    DOI: 10.14952/SEIKAGAKU.2021.930521

  • His-Cys and Trp-Cys Cross-links Generated by Post-translational Chemical Modification Reviewed

    Nobutaka Fujieda

    Biosci. Biotech. Biochem.   84 ( 3 )   2020.07

     More details

  • His-Cys and Trp-Cys cross-links generated by post-translational chemical modification Invited Reviewed

    Nobutaka Fujieda

    Bioscience, Biotechnology, and Biochemistry   84 ( 3 )   445 - 454   2020.03( ISSN:0916-8451 ( eISSN:1347-6947

     More details

    Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    ABSTRACT

    Galactose oxidase and amine oxidase contain a cofactor which is generated by post-translational chemical modification to the corresponding amino acid side chains near the copper active center. Such cofactors provide proteins unusual catalytic ability that canonical amino acids cannot exert as well as their structural stability, and thereby are called as protein-derived cofactors. These cofactors and modifications are mostly derived from aromatic amino acid residues, especially Tyr, Trp, and His. Current information about unusual cofactors derived from two of those, heteroaromatic residues (Trp and His) is summarized, especially chemical properties and maturation process of the cross-links between cysteine and heteroaromatic amino acids (His–Cys and Trp–Cys cross-links).

    Abbreviations: FMN: flavin mononucleotide; FAD: flavin adenine nucleotide; RNA: ribonucleic acid; PDC: protein-derived cofactor; GFP: green fluorescent protein; MIO: 3,5-dihydro-5-methylidene-4-imidazol-4-one; LTQ: lysyl tyrosylquinone; CTQ: cysteine tryptophylquinone; TTQ: tryptophan tryptophylquinone; E.coli: Escherichia coli; WT: wild type.

    DOI: 10.1080/09168451.2019.1696178

  • 非ヘム人工金属酵素の機能創製

    藤枝伸宇

    化学と工業   72 ( 12 )   1051 - 1052   2019.12

     More details

    Authorship:Lead author, Last author, Corresponding author  

▼display all

Presentations

  • Artificial Metallolyases with Pliable Copper Center for Stereoselective Michael Addition Invited

    Nobutaka Fujieda

    Pre-ISBC2024 Symposium in Nara  2024.04 

  • 酵素をだましてわがままな合成試薬開発 Invited

    藤枝伸宇

    CSJ化学フェスタ2023  2023.10 

  • Artificial Mononuclear Non-heme Metalloenzymes for Stereoselective Synthesis Invited Domestic conference

    藤枝伸宇

    錯体化学討論会シンポジウムS6  2023.09 

  • 柔軟な金属中心を有する非ヘム金属酵素 Invited Domestic conference

    藤枝伸宇

    第34回日本微量元素学会学術集会(BRTE2023)  2023.09 

     More details

    Presentation type:Oral presentation (invited, special)  

  • Simple Designing Artificial Non-heme Metalloenzymes and Their Application to Stereoselective Synthesis Invited

    Nobutaka Fujieda

    The 14th International Symposium of Advanced Energy Science  2023.08 

     More details

    Presentation type:Oral presentation (invited, special)  

  • ミネラル・金属を含むバイオファクター:その巧妙なつくられ方とはたらき メラニン生成酵素チロシナーゼの成熟

    藤枝 伸宇

    ビタミン  2022.09  (公社)日本ビタミン学会

  • Stereoselective Synthesis with Protein as Metal Ligands Invited

    Nobutaka Fujieda

    The 15th International Conference on Cutting-Edge Organic Chemistry in Asia  2022.07 

     More details

    Presentation type:Oral presentation (invited, special)  

  • タンパク質金属配位子を使った反応開発 Invited Domestic conference

    藤枝伸宇

    第90回白鷺セミナー  2021.12 

     More details

    Presentation type:Oral presentation (invited, special)  

  • タンパク質金属配位子を使った反応創製 Invited Domestic conference

    藤枝伸宇

    高難度物質変換反応の開発を指向した精密制御反応の創出 事後シンポジウム  2021.11 

     More details

    Presentation type:Oral presentation (invited, special)  

  • タンパク質金属配位子を使った反応制御 Invited Domestic conference

    藤枝伸宇

    2021年度 有機合成化学講演会 合成有機化学のフロンティア  2021.06 

     More details

    Presentation type:Oral presentation (invited, special)  

  • チロシナーゼにおける銅イオンの位置遷移 Invited Domestic conference

    藤枝伸宇

    日本農芸化学会2020年度大会  2021.03 

     More details

    Presentation type:Oral presentation (invited, special)  

  • 「生命金属科学」分野の創成による生体内金属動態の統合的研究:生命金属研究のさらなる発展に向けて チロシナーゼの成熟と反応機構

    藤枝 伸宇

    Biomedical Research on Trace Elements  2020.11  (一社)日本微量元素学会

  • Development of Artificial Metalloenzymes with a Small Barrel Protein Invited International conference

    FUJIEDA Nobutaka

    4th International Symposium on Precisely Designed Catalysts with Customized Scaffolding  2019.12 

     More details

    Presentation type:Oral presentation (invited, special)  

  • Development of Artificial Metalloenzymes with a Small Barrel Protein Invited International conference

    Nobutaka Fujieda

    4th International Symposium on Precisely Designed Catalysts with Customized Scaffolding  2019.12 

     More details

    Presentation type:Oral presentation (invited, special)  

  • Artificial Metalloenzymes Bearing a Small Barrel Protein Invited International conference

    FUJIEDA Nobutaka

    第10回日英シンポジウム『生命を理解するための化学』 ―Chemistry for Complex Biological Systems―  2019.09 

     More details

    Presentation type:Poster presentation  

  • Artificial Metalloenzymes Bearing a Cupin Fold Invited International conference

    FUJIEDA Nobutaka

    ArtZymes 2.0  2019.08 

     More details

    Presentation type:Oral presentation (keynote)  

  • Cupin Superfamily Proteins as Versatile Macromolecular Ligands Invited International conference

    FUJIEDA Nobutaka

    15th International Symposium on Applied Bioinorganic Chemistry  2019.06 

     More details

    Presentation type:Oral presentation (invited, special)  

  • Development of Artificial Metalloenzymes Using a Cupin Protein as a Metal-binding Platform Invited International conference

    FUJIEDA Nobutaka

    The International Symposium on Biofunctional Chemistry  2019.06 

     More details

    Presentation type:Oral presentation (invited, special)  

  • 高立体選択的C-C結合反応を触媒する人工金属酵素の創製

    藤枝 伸宇

    日本応用酵素協会誌  2019.03  (公財)日本応用酵素協会

▼display all

Industrial Property Rights

  • アルコールの製造方法

    藤枝伸宇・森井孝

     More details

    property_type:Patent 

  • タンパク質によるタングステンの回収

    藤枝伸宇

     More details

    property_type:Patent 

    Application no:特願2023-132888 

Outline of collaborative research (seeds)

  • タングステン、モリブデン、バナジウムの海洋回収

  • 高選択的不斉合成

  • 低級アルカンのバイオ資化

Charge of on-campus class subject

  • Bionanotechnology

    2021    

  • Biophysical Chemistry II

    2021    

  • Topics in Biophysical Chemistry

    2021    

  • Biophysical Chemistry I

    2021    

  • Introduction to Life, Environment, and Advanced Sciences

    2021    

  • General Principles of Applied Biological Chemistry

    2021    

  • Laboratory : Biophysical Chemistry

    2021   Practical Training  

  • Reviews for Applied Life Sciences

    2021    

▼display all

Charge of off-campus class subject

  • 生物物理化学特論

  • 生物物理化学実験

  • 生物物理化学

    Institution:Osaka Prefecture University

  • 物理化学実験

  • 有機化学実験

  • 情報活用基礎

  • 分析化学実験

  • 共通教育化学実験

  • バイオナノテクノロジー

▼display all

Social Activities

  • 光ナノサイエンス・マテリアル特別講義

    Role(s): Lecturer

    奈良先端技術大学院大学  2022.10

     More details

    Type:Visiting lecture

  • 学問発見講座

    Role(s): Lecturer

    茨木高校  2022.07

     More details

    Type:Visiting lecture

  • 第12回大阪府生徒研究発表会~大阪サイエンスデイ~第1部

    Role(s): Consultant

    天王寺高校スーパーサイエンスハイスクール  2019.10

  • 第11回大阪府生徒研究発表会~大阪サイエンスデイ~第1部

    Role(s): Consultant

    天王寺高校スーパーサイエンスハイスクール  2018.10

     More details

    Type:Research consultation

Media Coverage

  • Chem-Station スポットライトリサーチ:銅中心が動く人工非ヘム金属酵素の簡便な構築に成功- Internet

    https://www.chem-station.com/blog/2023/04/am.html  2023.04

  • 体を動かす「エンジン」、酵素の複雑なカラクリを明らかに Internet

    若手研究が世界を変える! みらいぶっく学問・大学ナビ  https://miraibook.jp/researcher/s2448s  2020

  • 生体内の酵素の特性を利用し、効率的な物質生産を目指す Internet

    マイナビニュース  https://news.mynavi.jp/kikaku/20201021-1427172/  2020

  • 人工金属酵素が世界を変える!? Internet

    MICHI?TAKE!PLUS  http://michitake.osakafu-u.ac.jp/2020/01/08/nobutaka _fujieda/  2020

Academic Activities

  • 第73回錯体化学討論回シンポジウムS6 実行委員長

    Role(s): Planning, management, etc.

    錯体化学会  2023.09

     More details

    Type:Competition, symposium, etc. 

  • 日本農芸化学会関西支部 第525回講演会 世話人代表

    Role(s): Planning, management, etc.

    日本農芸化学会関西支部  2023.07

     More details

    Type:Academic society, research group, etc. 

  • 10th Asian Biological Inorganic Chemistry Conference (AsBIC-10), Committee & Session organizers

    Role(s): Planning, management, etc.

    10th Asian Biological Inorganic Chemistry Conference (AsBIC-10)  2022.11 - 2022.12

     More details

    Type:Competition, symposium, etc. 

  • 第90回白鷺セミナー 世話人

    Role(s): Planning, management, etc.

    白鷺セミナー  2021.12

     More details

    Type:Academic society, research group, etc. 

  • ChemRxiv

    2021.10 - Now

  • 第69回錯体化学討論回シンポジウムS2 実行委員

    Role(s): Planning, management, etc.

    Japan Society of Coordination Chemistry  2019.09

     More details

    Type:Competition, symposium, etc. 

  • 15th International Symposium on Applied Bioinorganic Chemistry, Local organizing committee

    Role(s): Planning, management, etc.

    2019.06

  • 第4回バイオ関連化学シンポジウム若手フォーラム 世話人

    Role(s): Planning, management, etc.

    日本化学会 生体関連化学部会  2016.09

     More details

    Type:Academic society, research group, etc. 

  • 第65回錯体化学討論回シンポジウムS2 実行委員長

    Role(s): Planning, management, etc.

    Japan Society of Coordination Chemistry  2015.09

     More details

    Type:Competition, symposium, etc. 

▼display all