Life Science / Bioorganic chemistry

Nanotechnology/Materials / Bio chemistry

Nanotechnology/Materials / Inorganic/coordination chemistry

Nanotechnology/Materials / Chemistry and chemical methodology of biomolecules

第99回春季年会 第33回 若い世代の特別講演証

藤枝 伸宇

2019.03   日本化学会  

大阪大学蛋白質研究所共同研究員

2013.04 - Now

Osaka University   Institute for Protein Research   Research Fellow

2013.04 - Now

バーゼル大学化学科（スイス）客員教授

2013.04 - 2014.03

大阪大学大学院工学研究科生命先端工学専攻・助教

2009.04 - 2017.03

京都大学エネルギー理工学研究所・附属エネルギー複合機構研究センター共同研究者

2007.04 - 2009.03

Copper–Oxygen Dynamics in the Tyrosinase Mechanism

Nobutaka Fujieda, Kyohei Umakoshi, Yuta Ochi, Yosuke Nishikawa, Sachiko Yanagisawa, Minoru Kubo, Genji Kurisu, Shinobu Itoh

Angewandte Chemie   132 ( 32 )   13487 - 13492   2020.05（ ISSN:0044-8249 ） （ eISSN:1521-3757 ）

Cupin Variants as a Macromolecular Ligand Library for Stereoselective Michael Addition of Nitroalkanes Reviewed

Nobutaka Fujieda, Haruna Ichihashi, Miho Yuasa, Yosuke Nishikawa, Genji Kurisu, Shinobu Itoh

Angewandte Chemie International Edition   59 ( 20 )   7717 - 7720   2020.05（ ISSN:1433-7851 ） （ eISSN:1521-3773 ）

His-Cys and Trp-Cys Cross-links Generated by Post-translational Chemical Modification Nobutaka Fujieda Reviewed

Nobutaka Fujieda

Biosci. Biotech. Biochem. 雑誌   84 ( 3 )   2020.03

Tyrosinases in Organic Chemistry: a Versatile Tool for the α-Arylation of β-Dicarbonyl Compounds Reviewed

Roxanne Krug, Dennis Schröder, Jan Gebauer, Sanel Suljić, Yuma Morimoto, Nobutaka Fujieda, Shinobu Itoh, and Jörg Pietruszkaa

Euro. J. Org. Chem.   2018.07

Did catalytic amyloid play the significant role in chemical evolution? Reviewed

Nobutaka Fujieda

Viva Origino   2018.04

銅含有モノオキシゲナーゼによる酸素分子の活性化機構 Reviewed

伊東　忍・藤枝伸宇

生化学   2018.03

Geometric Effects on O–O bond Scission of Copper(II)-alkylperoxide Reviewed

Tsukasa Abe, Yuma Morimoto, Kaoru Mieda, Hideki Sugimoto, Nobutaka Fujieda, Takashi Ogura, and Shinobu Itoh

J. Inorg. Biochem.   2017.12

Tetrahedral Copper(II) Complexes with a Labile Coordination Site Supported by a tris-Tetramethylguanidinato Ligand

Ikuma Shimizu, Yuma Morimoto, Dieter Faltermeier, Marion Kerscher, Sayantan Paria, Tsukasa Abe, Hideki Sugimoto, Nobutaka Fujieda, Kaori Asano, Takeyuki Suzuki, Peter Comba, and Shinobu Itoh

Inorg. Chem.   2017.10

Generation and Characterisation of a Stable Nickel(II)-Aminoxyl Radical Complex Reviewed

Arnaud Parrot, Yuma Morimoto, Sayantan Paria, Hideki Sugimoto, Nobutaka Fujieda, and Shinobu Itoh

Dalton Trans.   2017.09

A Well-Defined Osmium-Cupin Complex: Hyperstable Artificial Osmium Peroxygenase Reviewed

Nobutaka Fujieda, Takumi Nakano, Yuki Taniguchi, Haruna Ichihashi, Hideki Sugimoto, Yuma Morimoto, Yosuke Nishikawa, Genji Kurisu, and Shinobu Itoh

J. Am. Chem. Soc. 雑誌   139 ( 14 )   2017.04

Controlling dicopper protein functions Reviewed

Nobutaka Fujieda and Shinobu Itoh

Bull. Chem. Soc. Jpn.   2016.03

Enzyme Repurposing of a Hydrolase as an Emergent Peroxidase upon Metal Binding Reviewed

Nobutaka Fujieda, Jonas Schätti, Edward Stuttfeld, Kei Ohkubo, Timm Maier, and Thomas R. Ward

Chem. Sci.   2015.11

Generation, Characterization, and Reactivity of a CuII−Alkylperoxide/Anilino Radical Complex: Insight into the O−O Bond Cleavage Mechanism Reviewed

Sayantan Paria, Takehiro Ohta, Yuma Morimoto, Takashi Ogura, Hideki Sugimoto, Nobutaka Fujieda, Kei Goto, Kaori Asano, Takeyuki Suzuki, and Shinobu Itoh

J. Am. Chem. Soc.   2015.09

Direct Hydroxylation of Benzene to Phenol Using Hydrogen Peroxide Catalyzed by Nickel Complexes Supported by Pyridylalkylamine Ligands Reviewed

Yuma Morimoto, Shuji Bunno, Nobutaka Fujieda, Hideki Sugimoto, and Shinobu Itoh

J. Am. Chem. Soc.   2015.07

Redox behavior of novel nickel and palladium complexes supported by trianionic non-innocent ligand containing beta-diketiminate and phenol groups Reviewed

Yuma Morimoto, June Takaichi, Shinichi Hanada, Kei Ohkubo, Hideki Sugimoto, Nobutaka Fujieda, Shunichi Fukuzumi and Shinobu Itoh

J. Porphyrins Phthalocyanines   2015.06

Geometric Control of Nuclearity in Copper(I)/Dioxygen Chemistry Reviewed

Tsukasa Abe, Yuma Morimoto, Tetsuro Tano, Kaoru Mieda, Hideki Sugimoto, Nobutaka Fujieda, Takashi Ogura, and Shinobu Itoh

Inorg. Chem.   2014.12

Redox Chemistry of Nickel(II)-Complexes Supported by a Series of Non-innocent beta-Diketiminate Ligands Reviewed

June Takaichi, Yuma Morimoto, Kei Ohkubo, Chizu Shimokawa, Takayuki Hojo, Seiji Mori, Haruyasu Asahara, Hideki Sugimoto, Nobutaka Fujieda, Nagatoshi Nishiwaki, Shunichi Fukuzumi, and Shinobu Itoh

Inorg. Chem.   2014.10

二核銅タンパク質の機能解明と触媒への応用 Reviewed

伊東　忍・藤枝伸宇

触媒年鑑   2014.08

Redox Properties of Mononuclear Copper(II)-Superoxide Complex Reviewed

Tetsuro Tano, Yuri Okubo, Atsushi Kunishita, Minoru Kubo, Hideki Sugimoto, Nobutaka Fujieda, Takashi Ogura, and Shinobu Itoh

Inorg. Chem.   2013.12

Crystal Structures of Copper-Depleted and Copper-Bound Fungal Pro-Tyrosinase: Insights Into Endogenous Cysteine-Dependent Copper Incorporation Reviewed

Nobutaka Fujieda, Shintaro Yabuta, Takuya Ikeda, Takuji Oyama, Norifumi Muraki, Genji Kurisu, and Shinobu Itoh

J. Biol. Chem.   2013.09

Copper Complexes of the Non-innocent beta-Diketiminate Ligand Containing Phenol Groups Reviewed

June Takaichi, Kei Ohkubo, Hideki Sugimoto, Motohiro Nakano, Daisuke Usa, Hiroaki Maekawa, Nobutaka Fujieda, Nagatoshi Nishiwaki, Shu Seki, Shunichi Fukuzumi, and Shinobu Itoh

Dalton Trans.   2013.07

Activation Mechanism of MelB Tyrosinase from Aspergillus oryzae by Acidic Treatment Reviewed

Nobutaka Fujieda, Michiaki Murata, Shintaro Yabuta, Takuya Ikeda, Chizu Shimokawa, Yukihiro Nakamura, Yoji Hata, Shinobu Itoh

J. Biol. Inorg. Chem.   2013.05

Active Site Models for the CuB Site of Peptidylclycine alpha-Hydroxylating Monooxygenase and Dopamine alpha-Monooxygenase Reviewed

Atsushi Kunishita, Mehmed Z. Ertem, Yuri Okubo, Tetsuro Tano, Hideki Sugimoto, Kei Ohkubo, Nobutaka Fujieda, Shuichi Fukuzumi, Christopher J. Cramer, and Shinobu Itoh

Inorg. Chem.   2012.12

Heterolytic Alkylhydroperoxide O–O Bond Cleavage by Copper(I) Complexes Reviewed

Tetsuro Tano, Hideki Sugimoto, Nobutaka Fujieda, and Shinobu Itoh

Eur. J. Inorg. Chem.   2012.10

Copper(I)-Dioxygen Reactivity in a Sterically Demanding Tripodal Tetradentate tren Ligand. Formation and Reactivity of a Mononuclear Copper(II) End-on Superoxo Complex Reviewed

Yuki Kobayashi, Kei Okubo, Takashi Nomura, Minoru Kubo, Nobutaka Fujieda, Hideki Sugimoto, Shunichi Fukuzumi, Kei Goto, Takashi Ogura, and Shinobu Itoh

Eur. J. Inorg. Chem.   2012.08

Artificial Dicopper Oxidase: Rational Reprogramming of Bacterial Metallo-beta-lactamase into a Catechol Oxidase Reviewed

Nobutaka Fujieda, Atsushi Hasegawa, Kenichi Ishihama, and Shinobu Itoh

Chem. Asian J.   2012.06

Multifunctions of MelB, a Fungal Tyrosinase from Aspergillus oryzae Reviewed

Nobutaka Fujieda, Michiaki Murata, Shintaro Yabuta, Takuya Ikeda, Chizu Shimokawa, Yukihiro Nakamura, Yoji Hata, and Shinobu Itoh

ChemBioChem.   2012.05

Reactivity of Copper(II)-alkylperoxo Complexes Reviewed

Tetsuro Tano, Mehmed Z. Ertem, Satoru Yamaguchi, Atsushi Kunishita, Hideki Sugimoto, Nobutaka Fujieda, Takashi Ogura, Christopher J. Cramer*, and Shinobu Itoh

Dalton Trans.   2011.12

Post-translational His-Cys Cross Linkage Formation in Tyrosinase Induced by Copper(II)-Peroxo Species Reviewed

Nobutaka Fujieda, Takuya Ikeda, Michiaki Murata, Sachiko Yanagisawa, Shigetoshi Aono, Kei Ohkubo, Satoshi Nagao, Takashi Ogura, Shun Hirota, Shunichi Fukuzumi, Yukihiro Nakamura, Yoji Hata, and Shinobu Itoh

J. Am. Chem. Soc.   2011.10

Reactions of Copper(II)-Phenol Systems with O2. Models for TPQ Biosynthesis in Copper Amine Oxidases Reviewed

Kae Tabuchi, Mehmed Z. Ertem, Hideki Sugimoto, Atsushi Kunishita, Tetsuro Tano, Nobutaka Fujieda, Christopher J. Cramer, and Shinobu Itoh

Inorg. Chem.   2011.05

Five Monomeric Hemocyanin Subunits from Portunus trituberculatus: Purification, Spectroscopic Characterization, and Quantitative Evaluation of Phenol Monooxygenase Activity Reviewed

Nobutaka Fujieda, Aki Yakiyama, and Shinobu Itoh

Biochem. Biophys. Acta   2010.10

銅タンパク質の水酸化触媒としての応用 Reviewed

藤枝伸宇・伊東　忍

化学工業   2010.09

Oxygenation Chemistry at Mononuclear Copper(II)-Hydroquinone Sysetm with O2 Reviewed

Kae Tabuchi, Hideki Sugimoto, Atsushi Kunishita, Nobutaka Fujieda, and Shinobu Itoh

Inorg. Chem.   2010.07

Catalytic Oxygenation of Phenols by Arthropod Hemocyanin, an Oxygen Carrier Protein, from Portunus trituberculatus Reviewed

Nobutaka Fujieda, Aki Yakiyama, and Shinobu Itoh

Dalton Trans.   2010.05

Site-directed mutation at residues near the catalytic site of histamine dehydrogenase from Nocardioides simplex and its effects on substrate inhibition Reviewed

Maiko Tsutsumi, Noriaki Tsuse, Nobutaka Fujieda, and Kenji Kano

J. Biochem.   2010.03

A single circularly permuted GFP sensor for inositol-1,3,4,5-tetrakisphosphate based on a split PH domain Reviewed

Reiko Sakaguchi, Takashi Endoh, Seigo Yamamoto, Kazuki Tainaka, Kenji Sugimoto, Nobutaka Fujieda, Shigeki Kiyonaka, Yasuo Mori, and Takashi Morii

Bioorg. Med. Chem.   2009.10

The Silent Form of Quinohemoprotein Amine Dehydrogenase from Paracoccus denitrificans Reviewed

Nobutaka Fujieda, Megumi Mori, Tokuji Ikeda, and Kenji Kano

Biosci. Biotech. Biochem.   2008.12

Bioelectrochemical Determination at Histamine Dehydrogenase-based Electrodes Reviewed

Ryhei Yamada. Nobutaka Fujieda, Maiko Tsutsumi, Seiya Tsujimura, Osamu Shirai and Kenji Kano

Electrochemistry   2008.10

Context-dependent Fluorescence Detection of a Phosphorylated Tyrosine Residue by a Ribonucleopeptide Reviewed

Tetsuya Hasegawa, Masaki Hagihara, Masatora Fukuda, Shun Nakano, Nobutaka Fujieda and Takashi Morii

J. Am. Chem. Soc.   2008.07

Thermodynamic Redox Properties Governing the Half-Reduction Characteristics of Histamine Dehydrogenase from Nocardioides simplex Reviewed

Maiko Tsutsumi, Nobutaka Fujieda, Seiya Tsujimura, Osamu Shirai and Kenji Kano

Biosci. Biotech. Biochem.   2008.05

Model Studies of 6,7-Indolequinone Cofactors of Quinohemoprotein Amine Dehydrogenases Reviewed

Murakami, Y., Yoshimoto, Nobutaka Fujieda, Kei Ohkubo, Kenji Kano, Shunichi Fukuzumi and Shinobu Itoh

J. Org. Chem.   2007.10

Production of Completely Flavinylated Histamine Dehydrogenase, Unique Covalently Bound Flavin and Iron-Sulfur Cluster Containing Enzyme, of Nocardioides simplex in Escherichia coli and Its Properties Reviewed

Nobutaka Fujieda, Noriaki Tsuse, Astuko Satoh, Tokuji Ikeda, and Kenji Kano

Biosci. Biotech. Biochem.   2005.10

Mediated Spectroelectrochemical Titration of Proteins for Redox Potential Measurements by a Separator-less One-compartment Bulk Electrolysis Method Reviewed

Seiya Tsujimura, Atsushi Kuriyama, Nobutaka Fujieda, Kenji Kano, and Tokuji Ikeda

Anal. Biochem.   2005.06

6-S-Cysteinyl Flavin Mononucleotide-containing Histamine Dehydrogenase from Nocardioides simplex: Molecular Cloning, Sequencing, Over-Expression and Characterization of Redox Centers of Enzyme Reviewed

Nobutaka Fujieda, Astuko Satoh, Noriaki Tsuse, Kenji Kano, and Tokuji Ikeda

Biochemistry   2004.10

Separator-less One-compartment Bulk Electrolysis with a Small Auxiliary Electrode and Its Application to Spectroelectrochemistry Reviewed

Atsushi Kuriyama, Moriaki Arasaki, Nobutaka Fujieda, Seiya Tsujimura, Kenji Kano, and Tokuji Ikeda

Electrochemistry   2004.06

DsbB Elicits a Red-shift of Bound Ubiquinone during the Catalysis of DsbA Oxidation Reviewed

Kenji Inaba, Yoh-hei Takahashi, Nobutaka Fujieda, Kenji Kano, Hideto Miyoshi, and Koreaki Ito

J. Biol. Chem.   2004.04

Redox Properties of Quinohemoprotein Amine Dehydrogenase from Paracoccus denitrificans Reviewed

Nobutaka Fujieda, Megumi Mori, Kenji Kano, and Tokuji Ikeda

Biochim. Biophys. Acta   2003.10

Spectroelectrochemical Evaluation of Redox Potentials of Cysteine Tryptophylquinone and Two Hemes c in Quinohemoprotein Amine Dehydrogenase from Paracoccus denitrificans Reviewed

Nobutaka Fujieda, Megumi Mori, Kenji Kano, and Tokuji Ikeda

Biochemistry   2002.12

Assembly of Dinuclear Copper Center in Tyrosinase and Hemocyanins [Metalloprotein Active Site Assembly (Handbook of Metalloproteins to the Encyclopedia of Inorganic and Bioinorganic Chemistry)]

Nobutaka Fujieda and Shinobu Itoh（ Role： Joint author）

John Wiley & Sons, Ltd  2017.09 

Fungal Pro-tyrosinase (Handbook of Metalloproteins to the Encyclopedia of Inorganic and Bioinorganic Chemistry)

Nobutaka Fujieda and Shinobu Itoh（ Role： Joint author）

John Wiley & Sons, Ltd  2015.06 

His-Cys and Trp-Cys cross-links generated by post-translational chemical modification Invited Reviewed

Nobutaka Fujieda

Bioscience, Biotechnology, and Biochemistry   84 ( 3 )   445 - 454   2020.03（ ISSN:0916-8451 ） （ eISSN:1347-6947 ）

非ヘム人工金属酵素の機能創製

藤枝伸宇

化学と工業   72 ( 12 )   1051 - 1052   2019.12

ミネラル・金属を含むバイオファクター:その巧妙なつくられ方とはたらき　メラニン生成酵素チロシナーゼの成熟

藤枝 伸宇

ビタミン  2022.09  (公社)日本ビタミン学会

Stereoselective Synthesis with Protein as Metal Ligands Invited

Nobutaka Fujieda

The 15th International Conference on Cutting-Edge Organic Chemistry in Asia  2022.07 

タンパク質金属配位子を使った反応開発 Invited Domestic conference

藤枝伸宇

第90回白鷺セミナー  2021.12 

タンパク質金属配位子を使った反応創製 Invited Domestic conference

藤枝伸宇

高難度物質変換反応の開発を指向した精密制御反応の創出 事後シンポジウム  2021.11 

タンパク質金属配位子を使った反応制御 Invited Domestic conference

藤枝伸宇

2021年度 有機合成化学講演会 合成有機化学のフロンティア  2021.06 

チロシナーゼにおける銅イオンの位置遷移 Invited Domestic conference

藤枝伸宇

日本農芸化学会2020年度大会  2021.03 

「生命金属科学」分野の創成による生体内金属動態の統合的研究:生命金属研究のさらなる発展に向けて　チロシナーゼの成熟と反応機構

藤枝 伸宇

Biomedical Research on Trace Elements  2020.11  (一社)日本微量元素学会

Development of Artificial Metalloenzymes with a Small Barrel Protein Invited International conference

FUJIEDA Nobutaka

4th International Symposium on Precisely Designed Catalysts with Customized Scaffolding  2019.12 

Development of Artificial Metalloenzymes with a Small Barrel Protein Invited International conference

Nobutaka Fujieda

4th International Symposium on Precisely Designed Catalysts with Customized Scaffolding  2019.12 

Artificial Metalloenzymes Bearing a Small Barrel Protein Invited International conference

FUJIEDA Nobutaka

第10回日英シンポジウム『生命を理解するための化学』 ―Chemistry for Complex Biological Systems―  2019.09 

Artificial Metalloenzymes Bearing a Cupin Fold Invited International conference

FUJIEDA Nobutaka

ArtZymes 2.0  2019.08 

Cupin Superfamily Proteins as Versatile Macromolecular Ligands Invited International conference

FUJIEDA Nobutaka

15th International Symposium on Applied Bioinorganic Chemistry  2019.06 

Development of Artificial Metalloenzymes Using a Cupin Protein as a Metal-binding Platform Invited International conference

FUJIEDA Nobutaka

The International Symposium on Biofunctional Chemistry  2019.06 

高立体選択的C-C結合反応を触媒する人工金属酵素の創製

藤枝 伸宇

日本応用酵素協会誌  2019.03  (公財)日本応用酵素協会

金属中心のミクロ転位を誘起する人為酵素設計法の構築

Grant-in-Aid for Scientific Research(B)  2024

カルコゲン含有補因子を自発形成する機能性タンパク質の創出

挑戦的研究(萌芽)  2024

生命機能化学特論A

2024   Weekly class   Graduate school

生命機能化学特殊講義A

2024   Intensive lecture   Undergraduate

生命機能化学インターンシップ

2024   Intensive lecture   Undergraduate

生物物理化学2

2024   Weekly class   Undergraduate

生命機能化学研究プレゼンテーション

2024   Intensive lecture   Graduate school

生命機能化学キャリアデザイン

2024   Intensive lecture   Graduate school

生物物理化学実験

2024   Weekly class   Undergraduate

Bionanotechnology

2021    

Biophysical Chemistry II

2021    

Topics in Biophysical Chemistry

2021    

Biophysical Chemistry I

2021    

Introduction to Life, Environment, and Advanced Sciences

2021    

General Principles of Applied Biological Chemistry

2021    

Laboratory : Biophysical Chemistry

2021   Practical Training  

Reviews for Applied Life Sciences

2021    

有機化学実験

情報活用基礎

分析化学実験

共通教育化学実験

第69回錯体化学討論回シンポジウムS2 実行委員

Role(s)： Planning, management, etc.

Japan Society of Coordination Chemistry  2019.09

15th International Symposium on Applied Bioinorganic Chemistry, Local organizing committee

Role(s)： Planning, management, etc.

2019.06

第4回バイオ関連化学シンポジウム若手フォーラム 世話人

Role(s)： Planning, management, etc.

日本化学会 生体関連化学部会  2016.09

第65回錯体化学討論回シンポジウムS2 実行委員長

Role(s)： Planning, management, etc.

Japan Society of Coordination Chemistry  2015.09