Researcher Profiles - Matsumoto Takuya

写真a

Matsumoto Takuya

Organization

Graduate School of Engineering Division of Science and Engineering for Materials, Chemistry and Biology Associate Professor
School of Engineering Department of Chemical Engineering

Affiliation

Institute of Engineering

Affiliation campus

Nakamozu Campus

Position

Graduate School of Engineering Division of Science and Engineering for Materials, Chemistry and Biology

Associate Professor 2024.04 - Now
Graduate School of Engineering Division of Science and Engineering for Materials, Chemistry and Biology

Assistant Professor 2022.04 - 2024.03
School of Engineering Department of Chemical Engineering

Associate Professor 2024.04 - Now
School of Engineering Department of Chemical Engineering

Assistant Professor 2022.04 - 2024.03

Degree

博士（工学）（ Kobe University ）

Research Areas

Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Chemical reaction and process system engineering
Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering
Life Science / Applied microbiology
Life Science / Applied biochemistry

Research Interests

反応工学
微生物
酵素
生物化学工学
合成生物学
酵素工学

Research subject summary

微生物を用いた有用物質生産
酵素を用いた物質変換
微生物を用いたプラスチックリサイクル
微生物を用いた電気培養

Research Career

微生物を用いたプラスチックリサイクル

PETase, 大腸菌 Individual

2020 - Now
大腸菌を用いた有用物質生産技術の開発

Individual

2018.04 - Now
酵母を用いた有用物質生産技術の開発

Individual

2018.04 - Now
酵素を用いた物質変換技術の開発

Individual

2018.04 - Now

Professional Memberships

極限環境生物学会

2019.10 - Now Domestic
日本蛋白質科学会

2019.02 - Now Domestic
酵素工学研究会

2013.10 - Now Domestic
日本生物工学会

2010.03 - Now Domestic
化学工学会

2009.10 - Now Domestic

Awards

田中貴金属記念財団奨励賞

2022.03 田中貴金属記念財団
第7回新化学技術研究奨励賞

2018.07 新化学技術推進協会
21th Young Asian Biochemical Engineers Community ポスター賞

2015.10 Asian Federation of Biotechnology
17th Young Asian Biochemical Engineers Community ポスター賞

2011.10 Asian Federation of Biotechnology

Job Career (off-campus)

大阪公立大学大学院工学研究科物質化学生命系専攻化学工学分野

2022.04 - Now
Osaka Prefecture University

2018.04 - 2022.03
神戸大学大学院　科学技術イノベーション研究科特命助教

2016.04 - 2018.03
神戸大学　自然科学系先端融合研究環　重点研究部助教

2014.03 - 2016.03
日本学術振興会特別研究員（PD）

2013.10 - 2014.02
日本学術振興会特別研究員（DC2）

2012.04 - 2013.09

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Education

Kobe University Graduate School, Division of Engineering Doctor's Course Graduated/Completed

- 2013.09
Kobe University Graduate School, Division of Engineering Master's Course Graduated/Completed

2009.04 - 2011.03
Kobe University Faculty of Engineering Bachelor's Course Graduated/Completed

2005.04 - 2009.03

Papers

Introducing glutamic acid residues to acyl-ACP reductase to enhance alka(e)ne production in Escherichia coli: Computer-aided design and subsequent experimental validation

Jiahu Han, Takuya Matsumoto, Ryosuke Yamada, Hiroyasu Ogino

Biochemical and Biophysical Research Communications 745 151237 2025.01（ ISSN:0006291X ）

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Publishing type：Research paper (scientific journal)

DOI： 10.1016/j.bbrc.2024.151237

PubMed
Enhancing D-lactic acid production by optimizing the expression of D-LDH gene in methylotrophic yeast Komagataella phaffii

Yoshifumi Inoue, Ryosuke Yamada, Takuya Matsumoto, Hiroyasu Ogino

Biotechnology for Biofuels and Bioproducts 17 ( 1 ) 149 2024.12（ eISSN:2731-3654 ）

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Publishing type：Research paper (scientific journal)

DOI： 10.1186/s13068-024-02596-0

PubMed

Other URL： https://link.springer.com/article/10.1186/s13068-024-02596-0/fulltext.html
Improvement of lipid production from glucose/xylose mixed-sugar by the oleaginous yeast Lipomyces starkeyi through ultra-violet mutagenesis

Sota Kamba, Ryosuke Yamada, Takuya Matsumoto, Hiroyasu Ogino

Enzyme and Microbial Technology 183 110551 - 110551 2024.11（ ISSN:0141-0229 ）

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Publishing type：Research paper (scientific journal)

DOI： 10.1016/j.enzmictec.2024.110551

PubMed
Co-culture systems of microalgae and heterotrophic microorganisms: applications in bioproduction and wastewater treatment and elucidation of mutualistic interactions Reviewed

Miiku Takahashi, Ryosuke Yamada, Takuya Matsumoto, Hiroyasu Ogino

World Journal of Microbiology and Biotechnology 40 ( 11 ) 368 2024.10（ ISSN:0959-3993 ）（ eISSN:1573-0972 ）

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Publishing type：Research paper (scientific journal)

DOI： 10.1007/s11274-024-04173-x

PubMed

Other URL： https://link.springer.com/article/10.1007/s11274-024-04173-x/fulltext.html
Reshaping the substrate‐binding pocket of acyl‐ACP reductase to enhance the production of sustainable aviation fuel in <i>Escherichia coli</i> Reviewed

Jiahu Han, Takuya Matsumoto, Ryosuke Yamada, Hiroyasu Ogino

Biotechnology and Bioengineering 122 ( 1 ) 211 - 222 2024.10（ ISSN:0006-3592 ）（ eISSN:1097-0290 ）

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Publishing type：Research paper (scientific journal)

Abstract

To reduce carbon emissions and address environmental concerns, the aviation industry is exploring the use of sustainable aviation fuel (SAF) as an alternative to traditional fossil fuels. In this context, bio‐alkane is considered a potentially high‐value solution. The present study focuses on the enzymes acyl‐acyl carrier protein [ACP] reductase (AAR) and aldehyde‐deformylating oxygenase (ADO), which are crucial enzymes for alka(e)ne biosynthesis. By using protein engineering techniques, including semi‐rational design and site‐directed mutagenesis, we aimed to enhance the substrate specificity of AAR and improve alkane production efficiency. The co‐expression of a modified AAR (Y26G/Q40M mutant) with wild‐type ADO in Escherichia coli significantly increased alka(e)ne production from 28.92 mg/L to 167.30 mg/L, thus notably demonstrating a 36‐fold increase in alkane yield. This research highlights the potential of protein engineering in optimizing SAF production, thereby contributing to the development of more sustainable and efficient SAF production methods and promoting greener air travel.

DOI： 10.1002/bit.28863

PubMed
Co-utilization of microalgae and heterotrophic microorganisms improves wastewater treatment efficiency Reviewed

Miiku Takahashi, Yukino Karitani, Ryosuke Yamada, Takuya Matsumoto, Hiroyasu Ogino

Applied Microbiology and Biotechnology 108 ( 1 ) 468 2024.09（ ISSN:0175-7598 ）（ eISSN:1432-0614 ）

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Publishing type：Research paper (scientific journal)

DOI： 10.1007/s00253-024-13309-w

PubMed

Other URL： https://link.springer.com/article/10.1007/s00253-024-13309-w/fulltext.html
Induction of point and structural mutations in engineered yeast Saccharomyces cerevisiae improve carotenoid production Reviewed

Ryosuke Yamada, Kazuya Ando, Rumi Sakaguchi, Takuya Matsumoto, Hiroyasu Ogino

World Journal of Microbiology and Biotechnology 40 ( 7 ) 230 2024.06（ ISSN:0959-3993 ）（ eISSN:1573-0972 ）

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Publishing type：Research paper (scientific journal)

DOI： 10.1007/s11274-024-04037-4

PubMed

Other URL： https://link.springer.com/article/10.1007/s11274-024-04037-4/fulltext.html
Development of a metabolic engineering technology to simultaneously suppress the expression of multiple genes in yeast and application in carotenoid production Reviewed

Ryosuke Yamada, Chihiro Yamamoto, Rumi Sakaguchi, Takuya Matsumoto, Hiroyasu Ogino

World Journal of Microbiology and Biotechnology 40 ( 7 ) 227 2024.06（ ISSN:0959-3993 ）（ eISSN:1573-0972 ）

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Publishing type：Research paper (scientific journal)

DOI： 10.1007/s11274-024-04034-7

PubMed

Other URL： https://link.springer.com/article/10.1007/s11274-024-04034-7/fulltext.html
Improvement of cell growth in green algae Chlamydomonas reinhardtii through co-cultivation with yeast Saccharomyces cerevisiae Reviewed

Yukino Karitani, Ryosuke Yamada, Takuya Matsumoto, Hiroyasu Ogino

Biotechnology Letters 46 ( 3 ) 431 - 441 2024.04（ ISSN:0141-5492 ）（ eISSN:1573-6776 ）

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Publishing type：Research paper (scientific journal)

DOI： 10.1007/s10529-024-03483-2

PubMed

Other URL： https://link.springer.com/article/10.1007/s10529-024-03483-2/fulltext.html
UV mutagenesis improves growth potential of green algae in a green algae–yeast co-culture system Reviewed

Yukino Karitani, Ryosuke Yamada, Takuya Matsumoto, Hiroyasu Ogino

Archives of Microbiology 206 ( 2 ) 61 2024.01（ ISSN:0302-8933 ）（ eISSN:1432-072X ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

DOI： 10.1007/s00203-023-03796-2

PubMed

Other URL： https://link.springer.com/article/10.1007/s00203-023-03796-2/fulltext.html
Display of PETase on the Cell Surface of Escherichia coli Using the Anchor Protein PgsA Reviewed

Takuma Yamashita, Takuya Matsumoto, Ryosuke Yamada, Hiroyasu Ogino

Applied Biochemistry and Biotechnology 196 ( 8 ) 1 - 13 2024.01（ ISSN:02732289 ）

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Authorship：Corresponding author Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

DOI： 10.1007/s12010-023-04837-8

PubMed
Transition of stability of putidaredoxin reductase by introducing proline Reviewed

Taiki Okamura, Rina Aritomi, Takuya Matsumoto, Ryosuke Yamada, Hidehiko Hirakawa, Hiroyasu Ogino

Biochemical Engineering Journal 201 109139 - 109139 2024.01（ ISSN:1369703X ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

DOI： 10.1016/j.bej.2023.109139
Building a machine‐learning model to predict optimal mevalonate pathway gene expression levels for efficient production of a carotenoid in yeast Reviewed

Shun Shimazaki, Ryosuke Yamada, Yoshiki Yamamoto, Takuya Matsumoto, Hiroyasu Ogino

Biotechnology Journal 19 ( 1 ) e2300285 2024.01（ ISSN:18606768 ）（ eISSN:1860-7314 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Abstract

Simultaneous modification of the expression levels of many metabolic enzyme genes results in diverse expression ratios of these genes; however, the relationship between gene expression levels and chemical productivity remains unclear. However, clarification of this relationship is expected to improve the productivity of useful chemicals. Supervised machine learning is considered to be an effective means to clarify this relationship. In this study, to improve the productivity of carotenoids in yeast Saccharomyces cerevisiae, we aimed to build a machine‐learning model that can predict the optimal gene expression level for carotenoid production. First, we obtained data on the expression levels of mevalonate pathway enzyme genes and carotenoid production. Then, based on these data, we built a machine‐learning model to predict carotenoid productivity based on gene expression levels. The prediction accuracy of 0.6292 (coefficient of determination) was achieved using the test data. The maximum predicted carotenoid productivity was 4.3 times higher in the engineered strain than in the parental strain, suggesting that the expression levels of the mevalonate pathway enzyme genes tHMG1 and ERG8 have a particularly large impact on carotenoid productivity. This study could be one of the important achievements in addressing the uncertainty of genotype‐phenotype correlations, which is one of the challenges facing metabolic engineering strategies.

DOI： 10.1002/biot.202300285

PubMed

Other URL： https://onlinelibrary.wiley.com/doi/pdf/10.1002/biot.202300285
Sortase A-Mediated Enzyme Assembly on Multimeric Protein for Improving Mevalonate Production

Munenori Hashimoto, Masahiro Fujitani, Takuya Matsumoto, Ryosuke Yamada, Hiroyasu Ogino

Synthetic Biology and Engineering 2 ( 4 ) 10017 - 10017 2024（ ISSN:2958-9053 ）

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Authorship：Corresponding author Publishing type：Research paper (scientific journal)

DOI： 10.70322/sbe.2024.10017
Enhancing 3-hydroxypropionic acid production in Saccharomyces cerevisiae through enzyme localization within mitochondria Reviewed

Takuya Matsumoto, Takashi Otani, Ryosuke Yamada, Hiroyasu Ogino

Biochemical and Biophysical Research Communications 680 1 - 6 2023.11（ ISSN:0006-291X ）

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Authorship：Lead author,　Corresponding author Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

DOI： 10.1016/j.bbrc.2023.09.010

PubMed
Engineering acyl-ACP reductase with fusion tags enhances alka(e)ne synthesis in Escherichia coli. Reviewed

Jiahu Han, Koki Asano, Takuya Matsumoto, Ryosuke Yamada, Hiroyasu Ogino

Enzyme and microbial technology 168 110262 - 110262 2023.05（ ISSN:01410229 ）

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Authorship：Corresponding author Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Alka(e)nes are high-value chemicals with a potentially broad range of industrial applications because of their following advantages: (1) chemical and structural resemblance to petroleum hydrocarbons and (2) higher energy density and hydrophobicity than those of other biofuels. The low yield of bio-alka(e)nes, however, hinders their commercial application. The activity and solubility of acyl carrier protein (ACP) reductase (AAR) affect alka(e)ne biosynthesis in cyanobacteria. The enhancement of the activity and concentration of soluble AAR through genetic and process engineering can improve bio-alka(e)ne yield. Although fusion tags are used to enhance the expression or solubility of recombinant proteins, their effectiveness in improving the production of bio-alka(e)nes has not yet been reported. Fusion tags can be used to improve the amount or activity of soluble AAR in Escherichia coli and to increase the yield of alka(e)nes in E. coli cells co-expressing aldehyde deformylating oxygenase (ADO). Hence, in the present study, histidine (His6/His12), thioredoxin (Trx), maltose-binding protein (MBP), and N-utilization substance (NusA) were used as AAR fusion tags. The strain expressing SeAAR with His12 tag and NpADO showed a 7.2-fold higher yield of alka(e)nes than the strain expressing AAR without fusion tag and NpADO. The highest titer of alka(e)nes (194.78 mg/L) was achieved with the His12 tag.

DOI： 10.1016/j.enzmictec.2023.110262

PubMed
Construction of a machine-learning model to predict the optimal gene expression level for efficient production of d-lactic acid in yeast Reviewed

Yoshiki Yamamoto, Ryosuke Yamada, Takuya Matsumoto, Hiroyasu Ogino

World Journal of Microbiology and Biotechnology 39 ( 3 ) 69 2023.03（ ISSN:09593993 ）（ eISSN:1573-0972 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

DOI： 10.1007/s11274-022-03515-x

PubMed

Other URL： https://link.springer.com/article/10.1007/s11274-022-03515-x/fulltext.html
Identification of genes responsible for absorbing palladium ion in Escherichia coli. Reviewed

Matsumoto T, Tanaka Y, Kamino M, Yamada R, Konishi Y, Ogino H

2023.02 （ ISSN:0916-8451 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

DOI： 10.1093/bbb/zbad021

PubMed
Mevalonate production by electro-fermentation in Escherichia coli via Mtr-based electron transfer system Reviewed

Takuya Matsumoto, Kazuki Higuma, Ryosuke Yamada, Hiroyasu Ogino

Biochemical Engineering Journal 191 108772 - 108772 2023.02（ ISSN:1369703X ）

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Authorship：Lead author,　Corresponding author Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

DOI： 10.1016/j.bej.2022.108772
Promoting cell growth and characterizing partial symbiotic relationships in the co‐cultivation of green alga Chlamydomonas reinhardtii and Escherichia coli Reviewed

Ryosuke Yamada , Moe Yokota , Takuya Matsumoto , Ben Hankamer , Hiroyasu Ogino

Biotechnology Journal 18 ( 2 ) 2200099 2022.12

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal
Mitochondrial expression of metabolic enzymes for improving carotenoid production in Saccharomyces cerevisiae Reviewed

Takuya Matsumoto, Tomoki Osawa, Hikaru Taniguchi, Akira Saito, Ryosuke Yamada, Hiroyasu Ogino

Biochemical Engineering Journal 189 108720 - 108720 2022.12（ ISSN:1369-703X ）

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Authorship：Lead author Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

DOI： 10.1016/j.bej.2022.108720
Protein engineering to improve the stability of Thermomyces lanuginosus lipase in methanol Reviewed

Taiki Okamura, Yohei Nogami, Takuya Matsumoto, Ryosuke Yamada, Hiroyasu Ogino

Biochemical Engineering Journal 187 108659 - 108659 2022.11（ ISSN:1369-703X ）

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Authorship：Corresponding author Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

DOI： 10.1016/j.bej.2022.108659
Bioengineering for the industrial production of 2,3-butanediol by the yeast, Saccharomyces cerevisiae. Reviewed

Ryosuke Mitsui, Ryosuke Yamada, Takuya Matsumoto, Hiroyasu Ogino

World journal of microbiology & biotechnology 38 ( 3 ) 38 - 38 2022.01

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Owing to issues, such as the depletion of petroleum resources and price instability, the development of biorefinery related technologies that produce fuels, electric power, chemical substances, among others, from renewable resources is being actively promoted. 2,3-Butanediol (2,3-BDO) is a key compound that can be used to produce various chemical substances. In recent years, 2,3-BDO production using biological processes has attracted extensive attention for achieving a sustainable society through the production of useful compounds from renewable resources. With the development of genetic engineering, metabolic engineering, synthetic biology, and other research field, studies on 2,3-BDO production by the yeast, Saccharomyces cerevisiae, which is safe and can be fabricated using an established industrial-scale cultivation technology, have been actively conducted. In this review, we sought to describe 2,3-BDO and its derivatives; discuss 2,3-BDO production by microorganisms, in particular S. cerevisiae, whose research and development has made remarkable progress; describe a method for separating and recovering 2,3-BDO from a microbial culture medium; and propose future prospects for the industrial production of 2,3-BDO by microorganisms.

DOI： 10.1007/s11274-021-03224-x

PubMed
Improving carotenoid production in recombinant yeast, Saccharomyces cerevisiae, using ultrasound-irradiated two-phase extractive fermentation Reviewed

R. Yamada, Y. Ando, R. Mitsui, A. Mizobata, S. Yoshihara, H. Tokumoto, T. Matsumoto, H. Ogino

Engineering in Life Sciences 雑誌 2022.01

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Kind of work：Joint Work
Improving carotenoid production in recombinant yeast, Saccharomyces cerevisiae, using ultrasound-irradiated two-phase extractive fermentation.

Ryosuke Yamada, Yorichika Ando, Ryosuke Mitsui, Asuka Mizobata, Shizue Yoshihara, Hayato Tokumoto, Takuya Matsumoto, Hiroyasu Ogino

Engineering in life sciences 22 ( 1 ) 4 - 12 2022.01

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Carotenoids are hydrophobic compounds that exhibit excellent bioactivity and can be produced by recombinant S. cerevisiae. Irradiating microorganisms with ultrasonic waves increase the productivity of various useful chemicals. Ultrasonic waves are also used to extract useful chemicals that accumulate in microbial cells. In this study, we aimed to improve the carotenoid production efficiency of a recombinant S. cerevisiae using an ultrasonic-irradiation based two-phase extractive fermentation process. When isopropyl myristate was used as the extraction solvent, a total of 264 mg/L of carotenoid was produced when batches were subjected to ultrasonic-irradiation at 10 W, which was a 1.3-fold increase when compared to the control. Transcriptome analysis suggested that one of the reasons for this improvement was an increase in the number of living cells. In fact, after 96 h of fermentation, the number of living cells increased by 1.4-fold upon irradiation with ultrasonic waves. Consequently, we succeeded in improving the carotenoid production in a recombinant S. cerevisiae strain using a ultrasonic-irradiated two-phase extractive fermentation and isopropyl myristate as the solvent. This fermentation strategy has the potential to be widely applied during the production of hydrophobic chemicals in recombinant yeast, and future research is expected to further develop this process.

DOI： 10.1002/elsc.202100051

PubMed
The synthesis of L-glycyl-L-tyrosine derivatives using organic-solvent stable PST-01 protease from Pseudomonas aeruginosa PST-01 Reviewed

T. Matsumoto, R. Kitayama, R. Yamada, H. Ogino

Process Biochemistry 2021.03
Improvement of 2,3-butanediol tolerance in Saccharomyces cerevisiae by using a novel mutagenesis strategy Reviewed

A. Mizobata, R. Mitsui, R. Yamada, T. Matsumoto, S. Yoshihara, H. Tokumoto, H. Ogino

Journal of Bioscience and Bioengineering 雑誌 2021.03
Improvement of 2,3-butanediol tolerance in Saccharomyces cerevisiae by using a novel mutagenesis strategy.

Asuka Mizobata, Ryosuke Mitsui, Ryosuke Yamada, Takuya Matsumoto, Shizue Yoshihara, Hayato Tokumoto, Hiroyasu Ogino

Journal of bioscience and bioengineering 131 ( 3 ) 283 - 289 2021.03

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Publishing type：Research paper (scientific journal) International / domestic magazine：Domestic journal

Although the yeast Saccharomyces cerevisiae has been used to produce various bio-based chemicals, including solvents and organic acids, most of these products inhibit yeast growth at high concentrations. In general, it is difficult to rationally improve stress tolerance in yeast by modifying specific genes, because many of the genes involved in stress response remain unidentified. Previous studies have reported that various forms of stress tolerance in yeast were improved by introducing random mutations, such as DNA point mutations and DNA structural mutations. In this study, we developed a novel mutagenesis strategy that allows for the simultaneous performance of these two types of mutagenesis to construct a yeast variant with high 2,3-butanediol (2,3-BDO) tolerance. The mutations were simultaneously introduced into S. cerevisiae YPH499, accompanied by a stepwise increase in the concentration of 2,3-BDO. The resulting mutant YPH499/pol3δ/BD_392 showed 4.9-fold higher cell concentrations than the parental strain after 96 h cultivation in medium containing 175 g/L 2,3-BDO. Afterwards, we carried out transcriptome analysis to characterize the 2,3-BDO-tolerant strain. Gene ontology enrichment analysis with RNA sequence data revealed an increase in expression levels of genes related to amino acid metabolic processes. Therefore, we hypothesize that the yeast acquired high 2,3-BDO tolerance by amino acid function. Our research provides a novel mutagenesis strategy that achieves efficient modification of the genome for improving tolerance to various types of stressors.

DOI： 10.1016/j.jbiosc.2020.11.004

PubMed
The synthesis of l-glycyl-l-tyrosine derivatives using organic-solvent stable PST-01 protease from Pseudomonas aeruginosa PST-01

Takuya Matsumoto, Ruri Kitayama, Ryosuke Yamada, Hiroyasu Ogino

Process Biochemistry 102 186 - 189 2021.03（ ISSN:1359-5113 ）

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Publishing type：Research paper (scientific journal)

DOI： 10.1016/j.procbio.2021.01.005
Improvement of lactic acid tolerance by cocktail δ-integration strategy and identification of the transcription factor PDR3 responsible for lactic acid tolerance in yeast Saccharomyces cerevisiae.

Ryosuke Yamada, Yuki Kumata, Ryosuke Mitsui, Takuya Matsumoto, Hiroyasu Ogino

World journal of microbiology & biotechnology 37 ( 2 ) 19 - 19 2021.01（ ISSN:0959-3993 ）（ eISSN:1573-0972 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Although, yeast Saccharomyces cerevisiae is expected to be used as a host for lactic acid production, improvement of yeast lactic acid tolerance is required for efficient non-neutralizing fermentation. In this study, we optimized the expression levels of various transcription factors to improve the lactic acid tolerance of yeast by a previously developed cocktail δ-integration strategy. By optimizing the expression levels of various transcription factors, the maximum D-lactic acid production and yield under non-neutralizing conditions were improved by 1.2. and 1.6 times, respectively. Furthermore, overexpression of PDR3, which is known as a transcription factor involved in multi-drug resistance, effectively improved lactic acid tolerance in yeast. In addition, we clarified for the first time that high expression of PDR3 contributes to the improvement of lactic acid tolerance. PDR3 is considered to be an excellent target gene for studies on yeast stress tolerance and further researches are desired in the future.

DOI： 10.1007/s11274-020-02977-1

PubMed
Improvement of lactic acid tolerance by cocktail δ-integration strategy and identification of the transcription factor PDR3 responsible for lactic acid tolerance in yeast Saccharomyces cerevisiae

R. Yamada, Y. Kumata, R. Mitsui, T. Matsumoto, H. Ogino

Journal of Microbiology and Biotechnology 雑誌 2021.01

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Kind of work：Joint Work
Identification of genes responsible for reducing palladium ion in Escherichia coli Reviewed

T Matsumoto, M Kamino, R Yamada, Y Konishi, H Ogino

Journal of Biotechnology 著書 2020.12

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Kind of work：Joint Work
Construction of lactic acid-tolerant Saccharomyces cerevisiae by using CRISPR-Cas-mediated genome evolution for efficient D-lactic acid production Reviewed

R Mitsui, R Yamada, T Matsumoto, S Yoshihara, H Tokumoto, H Ogino

Applied Microbiology and Biotechnology 雑誌 2020.11

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Kind of work：Joint Work
Construction of lactic acid-tolerant Saccharomyces cerevisiae by using CRISPR-Cas-mediated genome evolution for efficient D-lactic acid production.

Ryosuke Mitsui, Ryosuke Yamada, Takuya Matsumoto, Shizue Yoshihara, Hayato Tokumoto, Hiroyasu Ogino

Applied microbiology and biotechnology 104 ( 21 ) 9147 - 9158 2020.11

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Lactic acid (LA) is chemically synthesized or fermentatively produced using glucose as substrate, mainly using lactic acid bacteria. Polylactic acid is used as a biodegradable bioplastic for packaging materials, medical materials, and filaments for 3D printers. In this study, we aimed to construct a LA-tolerant yeast to reduce the neutralization cost in LA production. The pHLA2-51 strain was obtained through a previously developed genome evolution strategy, and transcriptome analysis revealed the gene expression profile of the mutant yeast. Furthermore, the expression of the genes associated with glycolysis and the LA synthesis pathway in the LA-tolerant yeast was comprehensively and randomly modified to construct a D-LA-producing, LA-tolerant yeast. In detail, DNA fragments expressing thirteen genes, HXT7, HXK2, PGI1, PFK1, PFK2, FBA1, TPI1, TDH3, PGK1, GPM1, ENO2, and PYK2, and D-lactate dehydrogenase (D-LDH) from Leuconostoc mesenteroides were randomly integrated into the genomic DNA in the LA-tolerant yeast. The resultant engineered yeast produced about 33.9 g/L of D-LA from 100 g/L glucose without neutralizing agents in a non-neutralized condition and 52.2 g/L of D-LA from 100 g/L glucose with 20 g/L CaCO3 in a semi-neutralized condition. Our research provides valuable insights into non-neutralized fermentative production of LA. KEY POINTS: • Lactic acid (LA) tolerance of yeast was improved by genome evolution. • The transcription levels of 751 genes were changed under LA stress. • Rapid LA production with semi-neutralization was achieved by modifying glycolysis. • A versatile yeast strain construction method based on the CRISPR system was proposed.

DOI： 10.1007/s00253-020-10906-3

PubMed
Metabolic engineering of E. coli for improving mevalonate production to promote NADPH regeneration and enhance acetyl-CoA supply. Reviewed

Daichi Satowa, Ryosuke Fujiwara, Shogo Uchio, Mariko Nakano, Chisako Otomo, Yuuki Hirata, Takuya Matsumoto, Shuhei Noda, Tsutomu Tanaka, Akihiko Kondo

Biotechnology and bioengineering 117 ( 7 ) 2153 - 2164 2020.07（ ISSN:0006-3592 ）（ eISSN:1097-0290 ）

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Microbial production of mevalonate from renewable feedstock is a promising and sustainable approach for the production of value-added chemicals. We describe the metabolic engineering of Escherichia coli to enhance mevalonate production from glucose and cellobiose. First, the mevalonate-producing pathway was introduced into E. coli and the expression of the gene atoB, which encodes the gene for acetoacetyl-CoA synthetase, was increased. Then, the deletion of the pgi gene, which encodes phosphoglucose isomerase, increased the NADPH/NADP+ ratio in the cells but did not improve mevalonate production. Alternatively, to reduce flux toward the tricarboxylic acid cycle, gltA, which encodes citrate synthetase, was disrupted. The resultant strain, MGΔgltA-MV, increased levels of intracellular acetyl-CoA up to sevenfold higher than the wild-type strain. This strain produced 8.0 g/L of mevalonate from 20 g/L of glucose. We also engineered the sugar supply by displaying β-glucosidase (BGL) on the cell surface. When cellobiose was used as carbon source, the strain lacking gnd displaying BGL efficiently consumed cellobiose and produced mevalonate at 5.7 g/L. The yield of mevalonate was 0.25 g/g glucose (1 g of cellobiose corresponds to 1.1 g of glucose). These results demonstrate the feasibility of producing mevalonate from cellobiose or cellooligosaccharides using an engineered E. coli strain.

DOI： 10.1002/bit.27350

PubMed

Other URL： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/bit.27350
Construction of yeast producing patchoulol by global metabolic engineering strategy Reviewed

R Mitsui, R Nishikawa, R Yamada, T Matsumoto, H Ogino

Biotechnology and Bioengineerin 雑誌 2020.05

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Kind of work：Joint Work
Construction of yeast producing patchoulol by global metabolic engineering strategy. Reviewed

Ryosuke Mitsui, Riru Nishikawa, Ryosuke Yamada, Takuya Matsumoto, Hiroyasu Ogino

Biotechnology and bioengineering 117 ( 5 ) 1348 - 1356 2020.05

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Patchoulol is a sesquiterpene alcohol found in the leaves of the patchouli plant that can be extracted by steam distillation. Notably, patchoulol is an essential natural product frequently used in the chemical industry. However, patchouli produces an insignificant amount of patchoulol, not to mention steam distillation, and requires a lot of energy and time. Recombinant microorganisms that can be cultured in mild conditions and can produce patchoulol from renewable biomass resources may be a promising alternative. We previously developed the global metabolic engineering strategy (GMES), which produces a comprehensive metabolic modification in yeast, using the cocktail δ-integration method. In this study, we aimed to produce patchoulol by modifying engineered yeast. The expression of nine genes involved in patchoulol synthesis was modulated using GMES. Regarding patchoulol production, the resultant strain, YPH499/PAT167/MVA442, showed a concentration of 42.1 mg/L, a production rate of 8.42 mg/L/d, and a yield of 2.05 mg/g-glucose, respectably. These concentration values, production rate, and yield obtained through batch-fermentation in this study were high level when compared to previously reported recombinant microorganism studies. GMES could be used as a potential strategy for producing secondary metabolites from plants in recombinant Saccharomyces cerevisiae.

DOI： 10.1002/bit.27284

PubMed
Metabolic engineering of E. coli for improving mevalonate production to promote NADPH regeneration and enhance acetyl‐CoA supply Reviewed

Daichi Satowa, Ryosuke Fujiwara, Shogo Uchio, Mariko Nakano, Chisako Otomo, Yuuki Hirata, Takuya Matsumoto, Shuhei Noda, Tsutomu Tanaka, Akihiko Kondo

Biotechnology and Bioengineering 雑誌 2020.04

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Kind of work：Joint Work
N-linked glycosylation of thermostable lipase from Bacillus thermocatenulatus to improve organic solvent stability Reviewed

S Kajiwara, R Yamada, T Matsumoto, H Ogino

Enzyme and microbial technology 雑誌 2020.01

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Kind of work：Joint Work
n-Butylamine production from glucose using a transaminase-mediated synthetic pathway in Escherichia coli. Reviewed

Takuya Matsumoto, Yuki Mori, Tsutomu Tanaka, Akihiko Kondo

Journal of bioscience and bioengineering 129 ( 1 ) 99 - 103 2020.01

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Publishing type：Research paper (scientific journal) International / domestic magazine：Domestic journal

Bioamination methods using microorganisms have attracted much attention because of the increasing demand for environmentally friendly bioprocesses. n-Butylamine production from glucose in Escherichia coli was demonstrated in this study, which has never been reported because of the absence of n-butylamine-producing pathway in nature. We focused on a transaminase-mediated cascade for bioamination from an alcohol or aldehyde. The cascade can convert an alcohol or an aldehyde to the corresponding amine with l-alanine as an amine donor. Here, n-butyraldehyde, which is a metabolic intermediate in the n-butanol producing pathway, is a potential intermediate for producing n-butylamine using this cascade. Hence, the n-butanol-producing pathway and the transaminase-mediated cascade were combined into a synthetic metabolic pathway for producing n-butylamine from glucose. Firstly, we demonstrated the conversion of n-butanol to n-butylamine using a three enzyme-mediated cascade. n-Butanol was successfully converted to n-butylamine in 92% yield in the presence of l-alanine and ammonium chloride. Then, the n-butanol-producing pathway and transaminase-mediated cascade were introduced into E. coli. Using this system, n-butylamine was successfully produced from glucose as a carbon source at a concentration of 53.2 mg L-1 after 96 h cultivation using a ppc (phosphoenolpyruvate carboxylase)-deficient strain. To the best of our knowledge, this is the first report of the direct production of n-butylamine from glucose, and may provide a starting point for the development of microbial methods to produce other bioamines.

DOI： 10.1016/j.jbiosc.2019.06.015

PubMed
N-linked glycosylation of thermostable lipase from Bacillus thermocatenulatus to improve organic solvent stability. Reviewed

Shota Kajiwara, Ryosuke Yamada, Takuya Matsumoto, Hiroyasu Ogino

Enzyme and microbial technology 132 109416 - 109416 2020.01

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

A thermostable lipase from Bacillus thermocatenulatus was glycosylated by forming the consensus sequence (-NXS/T-) for N-linked glycosylation by site-directed mutagenesis. Among the eight BTL2 mutants including the consensus sequence, six BTL2 mutants, A277 N, A290 N, Y200 N, T236 N, T238 N, and P261 N, were glycosylated. Among the six mutants, glycosylated A277 N and T236 N showed higher stability in the presence of 25% (v/v) DMSO (74.3 and 72.8% of initial activity was remained after incubation at 45 °C for 20 h, respectively) than deglycosylated A277 N and T236 N (57.2 and 45.1% of initial activity was remained, respectively). These glycosylated mutants also showed higher remaining activity than wild-type BTL2 (56.0% of the initial activity were remained). Furthermore, the glycosylated mutant T236 N showed longer half-lives in the presence of 25% (v/v) ethylene glycol, DMSO, and DMF (161, 133, and 56.7 h at 45 °C, respectively) than deglycosylated mutant T236 N (107, 91.9, and 42.8 h, respectively). N-linked glycosylation may be a promising approach for preparing enzymes to retain their activity in the presence of organic solvents.

DOI： 10.1016/j.enzmictec.2019.109416

PubMed
Chemical treatments for modification and immobilization to improve the solvent-stability of lipase. Reviewed

Takuya Matsumoto, Ryosuke Yamada, Hiroyasu Ogino

World journal of microbiology & biotechnology 35 ( 12 ) 193 - 193 2019.11

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

Lipase is a lipolytic enzyme that catalyzes the hydrolysis of lipids and esterification reactions. Lipase has been utilized in industrial uses, food processing, and therapeutic applications as a biocatalyst. However, substrates of lipase are often insoluble in water, and this problem limits its utility. Lipases are also used in organic solvents where the solvent-stability of lipase is an important factor. There is a huge number of approaches that can be undertaken to improve the organic solvent-stability of lipases. For example, screening of solvent-tolerant lipase in nature and direct evolution of lipase using genetic engineering are some of the employed approaches. Here, we focus on approaches based on the chemical treatment of lipases for modification and immobilization. The solvent-stability of lipase was improved by the attachment of other molecules, such as surfactants, polymers, and carbohydrates. The immobilization of the enzyme is been known to be an effective approach for not only recycling the enzyme but also its stabilization. Several reports have demonstrated that the solvent-stability of lipase is also improved by immobilization. In this review, we provide an overview of the approaches used to improve the solvent-stability of lipase.

DOI： 10.1007/s11274-019-2777-8

PubMed
n-Butylamine production from glucose using a transaminase-mediated synthetic pathway in Escherichia coli. Reviewed

T. Matsumoto, Y. Mori, T. Tanaka*, A. Kondo

Journal of Bioscience and Bioengineering 雑誌 2019.06

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Kind of work：Joint Work
Modification of lipase from Candida cylindracea with dextran using the borane-pyridine complex to improve organic solvent stability. Reviewed

Shota Kajiwara, Kyohei Komatsu, Ryosuke Yamada, Takuya Matsumoto, Masahiro Yasuda, Hiroyasu Ogino

Journal of biotechnology 296 1 - 6 2019.04

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Publishing type：Research paper (scientific journal) International / domestic magazine：International journal

In this study, a commercial lipase derived from Candida cylindracea was chemically modified with dextran by conjugating ε-amine in the lysine residue with the carbonyl residue in oxidized dextran using the borane-pyridine complex as a reducing agent to increase the hydrophilicity of the microenvironment around the lipase in the presence of organic solvents. The degree of modification (53.2%), amount of dextran (0.66 g/g-lipase), specific activity (similar to that of the unmodified lipase), and stability in the presence of ethanol and 2-propanol (the half-lives were 2.24 and 1.86 times longer than those of the unmodified lipase) were higher for the lipase modified at pH 8.0 than for the lipases modified at other pH levels. Following modification with dextran at pH 8.0, the stability of the modified lipase was higher than that of the unmodified lipase in the presence of 25% (v/v) DMSO, ethanol, 2-propanol, toluene, n-hexane, and isooctane (the half-lives were 1.45, 2.24, 1.86, 1.76, 2.67 and 2.95 times longer than those of the unmodified lipase). Therefore, chemical modification with polysaccharides such as dextran using the borane-pyridine complex as a reducing agent could be a promising approach for improving the organic solvent stability of enzymes.

DOI： 10.1016/j.jbiotec.2019.02.009

PubMed
Modification of lipase from Candida cylindracea with dextran using the borane-pyridine complex to improve organic solvent stability Reviewed

Shota Kajiwara, Kyohei Komatsu, Ryosuke Yamada, Takuya Matsumoto, Masahiro Yasuda, Hiroyasu Ogino

Journal of biotechnology 雑誌 2019.04

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Kind of work：Joint Work
Improvement of the organic solvent stability of a commercial lipase by chemical modification with dextran Reviewed

Shota Kajiwara, Kyohei Komatsu, Ryosuke Yamada, Takuya Matsumoto, Masahiro Yasuda, Hiroyasu Ogino

Biochemical engineering journal 雑誌 2019.02

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Kind of work：Joint Work
Improvement of the organic solvent stability of a commercial lipase by chemical modification with dextran

Kajiwara, S., Komatsu, K., Yamada, R., Matsumoto, T., Yasuda, M., Ogino, H.

Biochemical Engineering Journal 142 2019

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Publishing type：Research paper (scientific journal)

DOI： 10.1016/j.bej.2018.11.003
Enhancing 3-hydroxypropionic acid production in combination with sugar supply engineering by cell surface-display and metabolic engineering of Schizosaccharomyces pombe Reviewed

Seiya Takayama, Aiko Ozaki, Rie Konishi, Chisako Otomo, Mayumi Kishida, Yuuki Hirata, Takuya Matsumoto, Tsutomu Tanaka, Akihiko Kondo

Microbial cell factories 雑誌 2018.12

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Kind of work：Joint Work
Streptavidin-hydrogel prepared by sortase A-assisted click chemistry for enzyme immobilization on an electrode Reviewed

T Matsumoto, Y Isogawa, T Tanaka, A Kondo

Biosensors and Bioelectronics 雑誌 99 56 - 61 2018.01
1, 5-Diaminopentane production from xylooligosaccharides using metabolically engineered Corynebacterium glutamicum displaying beta-xylosidase on the cell surface Reviewed

K Imao, R Konishi, M Kishida, Y Hirata, S Segawa, N Adachi, R Matsuura, Y Tsuge, T Matsumoto, T Tanaka, A Kondo

Bioresource technology 雑誌 245 1684 - 1691 2017.12
Metabolic engineering of Schizosaccharomyces pombe via CRISPR-Cas9 genome editing for lactic acid production from glucose and cellobiose Reviewed

A Ozaki, R Konishi, C Otomo, M Kishida, S Takayama, T Matsumoto, T Tanaka, A Kondo

Metabolic engineering communications 雑誌 5 60 - 67 2017.12
Sortase A-Mediated Metabolic Enzyme Ligation in Escherichia coli Reviewed

T Matsumoto, K Furuta, T Tanaka, A Kondo

ACS synthetic biology 雑誌 5 ( 11 ) 1284 - 1289 2016.10
Improvement of ectoine productivity by using sugar transporter-overexpressing Halomonas elongata Reviewed

K Tanimura, T Matsumoto, H Nakayama, T Tanaka, A Kondo

Enzyme and microbial technology 雑誌 89 63 - 68 2016.07
2, 3-Butanediol production from cellobiose using exogenous beta-glucosidase-expressing Bacillus subtilis Reviewed

K Tanimura, S Takashima, T Matsumoto, T Tanaka, A Kondo

Applied microbiology and biotechnology 雑誌 100 ( 13 ) 5781 - 5789 2016.07
Twigged streptavidin polymer as a scaffold for protein assembly Reviewed

T Matsumoto, Y Isogawa, K Minamihata, T Tanaka, A Kondo

Journal of biotechnology 雑誌 225 61 - 66 2016.05
Secretory production of tetrameric native full-length streptavidin with thermostability using Streptomyces lividans as a host Reviewed

S Noda, T Matsumoto, T Tanaka, A Kondo

Microbial cell factories 雑誌 14 ( 1 ) 2015.12
C‐Terminal‐oriented Immobilization of Enzymes Using Sortase A‐mediated Technique Reviewed

Y Hata, T Matsumoto, T Tanaka, A Kondo

Macromolecular bioscience 雑誌 15 ( 10 ) 1375 - 1380 2015.10
Multi-functional glycoside hydrolase: Blon_0625 from Bifidobacterium longum subsp. infantis ATCC 15697 Reviewed

T Matsumoto, S Shimada, Y Hata, T Tanaka, A Kondo

Enzyme and microbial technology 雑誌 68 10 - 14 2015.01
Two‐Stage Oxidation of Glucose by an Enzymatic Bioanode Reviewed

T Matsumoto, S Shimada, K Yamamoto, T Tanaka, A Kondo

Fuel Cells 雑誌 13 ( 6 ) 960 - 964 2013.12
Starchy biomass-powered enzymatic biofuel cell based on amylases and glucose oxidase multi-immobilized bioanode Reviewed

K Yamamoto, T Matsumoto, S Shimada, T Tanaka, A Kondo

New biotechnology 雑誌 30 ( 5 ) 531 - 535 2013.06
Site‐specific protein labeling with amine‐containing molecules using Lactobacillus plantarum sortase Reviewed

T Matsumoto, R Takase, T Tanaka, H Fukuda, A Kondo

Biotechnology journal 雑誌 7 ( 5 ) 642 - 648 2012.05
Sortase A-catalyzed site-specific coimmobilization on microparticles via streptavidin Reviewed

T Matsumoto, T Tanaka, A Kondo

Langmuir 雑誌 28 ( 7 ) 3553 - 3557 2012.02
Site-specific tetrameric streptavidin-protein conjugation using sortase A Reviewed

T Matsumoto, S Sawamoto, T Sakamoto, T Tanaka, H Fukuda, A Kondo

Journal of biotechnology 雑誌 152 ( 1-2 ) 37 - 42 2011.03

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Books and Other Publications

大腸菌によるレアメタルイオンの還元および吸着に関する遺伝子の探索

松本拓也, 荻野博康（ Role： Joint author）

シーエムシー･リサーチ 2024年3月 (ISBN: 9784910581507) 2024.03 （ ISBN:9784910581507 ）
微生物機能を活用したレアメタル・貴金属リサイクル

松本拓也, 荻野博康（ Role： Joint author , 大腸菌によるレアメタルイオンの還元および吸着に関する遺伝子の探索）

シーエムシー･リサーチ 2024.03 （ ISBN:9784910581507 ）
Sortase A-Assisted Metabolic Enzyme Ligation in Escherichia coli for Enhancing Metabolic Flux.

Takuya MATSUMOTO, Tsutomu TANAKA, Akihiko KONDO（ Role： Joint author）

Synthetic Biology, Methods in Molecular Biology, Humana Press, New York 2018.05

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Responsible for pages：1772. 125-136
Sortase Aを用いたタンパク質配向固定化技術の開発

松本　拓也、田中　勉（ Role： Joint author）

シーエムシー出版 2018.04 （ ISBN:9784781313269 ）

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Responsible for pages：第３章

MISC

ポリエチレンテレフタレート（PET）は生分解性？

松本拓也

日本生物工学会バイオミディア 98 ( 2 ) 2020.02

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Chemical treatments for modification and immobilization to improve the solvent-stability of lipase Reviewed

T Matsumoto, R Yamada, H Ogino

World Journal of Microbiology and Biotechnology 2019.12

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Engineering metabolic pathways in Escherichia coli for constructing a “microbial chassis” for biochemical production Reviewed

T Matsumoto, T Tanaka, A Kondo

Bioresource technology 245 1362 - 1368 2017.12
Enzyme‐mediated methodologies for protein modification and bioconjugate synthesis Reviewed

T Matsumoto, T Tanaka, A Kondo

Biotechnology journal 7 ( 9 ) 1137 - 1146 2012.09

Presentations

点変異と構造変異の導入による酵母のタンパク質生産性向上とカロテノイド生産への応用

山田亮祐, 井上義文, 仮谷柚希乃, 坂口瑠美, 松本拓也, 荻野博康

日本農芸化学会2025年度大会 2025.03
固定化微細藻類共培養系による排水処理

高橋美郁, 山田亮祐, 松本拓也, 荻野博康

日本農芸化学会2025年度大会 2025.03
カロテノイド生産酵母への変異導入における集積培養条件の検討

室井誠, 山田亮祐, 松本拓也, 荻野博康

化学工学会第90年会 2025.03
PET分解酵素を表層提示した大腸菌触媒の開発

藏内佑斗, 松本拓也, 山田亮祐, 荻野博康

化学工学会第90年会 2025.03
Komagataella phaffii代謝改変株へのUV変異導入によるD-乳酸生産性の向上

井上義文, 中村海斗, 山田亮祐, 松本拓也, 荻野博康

化学工学会第90年会 2025.03
Komagataella phaffiiにおけるD-LDH遺伝子の発現最適化によるメタノールからのD-乳酸生産性向上

井上義文, 山田亮祐, 松本拓也, 荻野博康

日本農芸化学会2025年度大会 2025.03
Engineering acyl-ACP reductase to enhance the alka(e)ne production in Escherichia coli

韓佳虎, 松本拓也, 山田亮祐, 荻野博康

化学工学会第90年会 2025.03
PET分解酵素を表層提示した大腸菌触媒の開発

藏内佑斗, 松本拓也, 山田亮祐, 荻野博康

酵素工学研究会第92回講演会 2024.11
酵母ミトコンドリアを利用した3-ヒドロキシプロピオン酸生産

中村あさ陽, 松本拓也, 山田亮祐, 荻野博康

超越分子システム第3回若手会 2024.10
アシル-ACP還元酵素の基質結合ポケットの再構築による大腸菌での持続可能な航空燃料生産の強化

韓佳虎, 松本拓也, 山田亮祐, 荻野博康

超越分子システム第3回若手会 2024.10
The surface design of Escherichia coli for efficient PET degradation

Takuya Matsumoto, Yuto Kurauchi, Ryosuke Yamada, Hiroyasu Ogino

29th Symposium of Young Asian Biological Engineers’ Community 2024.10

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Presentation type：Oral presentation (general)
Reshaping the substrate-binding pocket of acyl-ACP reductase to enhance the production of sustainable aviation fuel in Escherichia col

Jiahu Han, Takuya Matsumoto, Ryosuke Yamada, Hiroyasu Ogino

29th Symposium of Young Asian Biological Engineers’ Community 2024.10
PET分解酵素を表層提示した大腸菌触媒の開発

藏内佑斗, 松本拓也, 山田亮祐, 荻野博康

超越分子システム第3回若手会 2024.10
Optimization of D-LDH gene expression for D-lactic acid production from methanol by engineered Komagataella phaffii

Yoshifumi Inoue, Ryosuke Yamada, Takuya Matsumoto, Hiroyasu Ogino

29th Symposium of Young Asian Biological Engineers’ Community 2024.10
細胞外電子伝達系をリポソーム上で再現した新規反応キャリアーの構築

松本拓也

学術変革領域研究(A)「生物を凌駕する無細胞分子システムのボトムアップ構築学」第4回領域会議 2024.09
点変異と構造変異の導入による酵母の2,3-ブタンジオール耐性向上

中村海斗, 山田亮祐, 松本拓也, 荻野博康

化学工学会第55回秋季大会 2024.09
Reshaping the substrate-binding pocket of acyl-ACP reductase to enhance the production of sustainable aviation fuel in Escherichia coli

韓佳虎, 松本拓也, 山田亮祐, 荻野博康

化学工学会第55回秋季大会 2024.09
Komagataella phaffiiへのD-LDH遺伝子多コピー組込みによるメタノールからのD-乳酸生産性向上

井上義文, 山田亮祐, 松本拓也, 荻野博康

化学工学会第55回秋季大会 2024.09
酵母における複数遺伝子同時発現抑制技術の開発とβ-カロテン生産への応用

山田亮祐, 山本千尋, 松本拓也, 荻野博康

生物工学若手研究者の集い夏のセミナー 2024.07
微細藻類と従属栄養微生物との共培養による排水処理効率の向上

高橋美郁, 山田亮祐, 松本拓也, 荻野博康

生物工学若手研究者の集い夏のセミナー 2024.07
メタノール資化性酵母によるD-乳酸生産を目指したD-LDH発現の検討

井上義文, 山田亮祐, 松本拓也, 荻野博康

生物工学若手研究者の集い夏のセミナー 2024.07
UV変異導入による油脂酵母Lipomyces starkeyiの油脂生産向上 Domestic conference

神場創太, 山田亮祐, 松本拓也, 荻野博康

化学工学会第89年会 2024.03
分子動力学計算と比較生物学的手法を利用したクチナーゼへのメタノール耐性付与 Domestic conference

岡村大毅, 松本拓也, 山田亮祐, 荻野博康

化学工学会第89年会 2024.03
細胞内小器官内での酵素局在化を介した出芽酵母における3-ヒドロキシプロピオン酸生産の改善 Domestic conference

齋藤明, 松本拓也, 山田亮祐, 荻野博康

化学工学会第89年会 2024.03
PETaseによるPET前駆体合成および親水性物質との複合化による合成活性の向上 Domestic conference

蔭山諄, 松本拓也, 山田亮祐, 荻野博康

化学工学会第89年会 2024.03
Enhancement of bio-alka(e)nes production by Escherichia coli expressing engineered acyl-ACP reductases with N-terminal tags Domestic conference

韓佳虎, 松本拓也, 山田亮祐, 荻野博康

化学工学会第89年会 2024.03
Komagataella phaffiiによるメタノールからのD-乳酸生産を目指したD-LDH発現の検討 Domestic conference

井上義文, 山田亮祐, 松本拓也, 荻野博康

化学工学会第89年会 2024.03
D-乳酸生産メチロトローフ酵母と緑藻との共培養によるD-乳酸生産性向上 Domestic conference

井上義文, 仮谷柚希乃, 山田亮祐, 松本拓也, 荻野博康

日本微生物生態学会第36回浜松大会 2023.11
酵母を用いた酵素の細胞内小器官局在化による3-ヒドロキシプロピオン酸生産性の改善 Domestic conference

齋藤明, 松本拓也, 山田亮祐, 荻野博康

酵素工学研究会第90回講演会 2023.11
変異導入による緑藻・酵母共培養系における緑藻増殖能の向上 Domestic conference

仮谷柚希乃, 山田亮祐, 松本拓也, 荻野博康

日本微生物生態学会第36回浜松大会 2023.11
PETaseを細胞表層に提示した大腸菌の開発 Domestic conference

山下拓真, 松本拓也, 山田亮祐, 荻野博康

酵素工学研究会第90回講演会 2023.11
酵母との共培養による緑藻の増殖能向上 Domestic conference

仮谷柚希乃, 山田亮祐, 松本拓也, 荻野博康

化学工学会第54回秋季大会 2023.09
分子動力学計算を利用したクチナーゼへの有機溶媒耐性付与 Domestic conference

岡村大毅, 松本拓也, 山田亮祐, 荻野博康

化学工学会第54回秋季大会 2023.09
カロテノイド高生産酵母の作製を目指した機械学習モデルの構築 Domestic conference

島崎舜, 山田亮祐, 山本祥輝, 松本拓也, 荻野博康

化学工学会第54回秋季大会 2023.09
Engineering acyl-ACP reductase with fusion tags enhances alka(e)ne synthesis in Escherichia coli Domestic conference

2023.09
Electro-fermentation in Escherichia coli via Mtr-based electron transfer system International conference

T. Matsumoto, R. Sekiya, R. Yamada, H. Ogino

2023.07

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Improvement of methanol stability of cutinase from Saccharomonospora viridis International conference

T. Okamura, T. Matsumoto, R. Yamada, H. Ogino

The 28th Symposium of Young Asian Biological Engineers’ Community 2023.07

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アシルキャリヤータンパク質の高発現によるアルカンの生成に優れた大腸菌の開発 Domestic conference

松原武輝, 韓佳虎, 浅野紘輝, 松本拓也, 山田亮祐, 荻野博康

第25回化学工学会学生発表会 2023.03
複数遺伝子の同時発現抑制による酵母のβ-カロテン生産性向上 Domestic conference

山本千尋, 三ツ井良輔, 山田亮祐, 坂口瑠美, 松本拓也, 荻野博康

化学工学会第88年会 2023.03
基質特異性の異なるThermomyces lanuginosus lipaseライブラリの作製 Domestic conference

森岡りな, 野上洋平, 松本拓也, 山田亮祐, 荻野博康

化学工学会第88年会 2023.03
PETaseを細胞表層に提示した大腸菌の開発 Domestic conference

山下拓真, 松本拓也, 山田亮祐, 荻野博康

化学工学会第88年会 2023.03
酵母による高効率D-乳酸生産を目指した機械学習モデルの構築 Domestic conference

山本祥輝, 山田亮祐, 松本拓也, 荻野博康

第74回日本生物工学会大会 2022.10
Thermomyces lanuginosus由来のリパーゼへのメタノール耐性付与 Domestic conference

岡村大毅, 野上洋平, 松本拓也, 山田亮祐, 荻野博康

第24回化学工学会学生発表会 2022.03
有機溶媒耐性PST-01プロテアーゼを用いたアラニルアラニンの合成 Domestic conference

水野ひなた，松本拓也，山田亮祐，冨田健一，星野美奈子，荻野博康

化学工学会関西大会2021 2021.12

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Presentation type：Poster presentation
白金イオンの還元および吸着に関する大腸菌の遺伝子の探索 Domestic conference

鬼頭和也，松本拓也，山田亮祐，荻野博康

化学工学会関西大会2021 2021.12

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酵母を用いた酵素のミトコンドリア局在化による3-ヒドロキシプロピオン酸の生産 Domestic conference

大谷孝，松本拓也，山田亮祐，荻野博康

化学工学会関西大会2021 2021.12

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大腸菌の代謝改変によるバイオアルカンの生産性向上 Domestic conference

浅野紘輝，松本拓也，山田亮祐，荻野博康

化学工学会関西大会2021 2021.12

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点変異・構造変異同時導入によるカロテノイド高生産酵母の創製 Domestic conference

安藤和哉，山田亮祐，松本拓也，荻野博康

化学工学会関西大会2021 2021.12

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枯草菌との共培養による緑藻の増殖能の向上 Domestic conference

大山遥行，山田亮祐，松本拓也，荻野博康

化学工学会関西大会2021 2021.12

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大腸菌の代謝改変によるバイオアルカンの生産性向上

浅野紘輝, 松本拓也, 山田亮祐, 荻野博康

化学工学会関西大会2021 2021.12

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酵母を用いた酵素のミトコンドリア局在化による3-ヒドロキシプロピオン酸の生産

大谷孝, 松本拓也, 山田亮祐, 荻野博康

化学工学会関西大会2021 2021.12

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Transcriptome analysis of thermotolerant yeast mutant International conference

R. Mitsui, R. Yamada*, T. Matsumoto, S. Yoshihara, H. Tokumoto, H. Ogino

The 26th Symposium of Young Asian Biological Engineers’ Community 2021.11

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Presentation type：Oral presentation (invited, special)
点変異・構造変異同時導入による酵母の過酸化水素耐性およびカロテノイド生産性向上 Domestic conference

安藤和哉，山田亮祐，松本拓也，荻野博康

極限環境生物学会2021年度（第22回）年会 2021.11

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Transcriptome analysis of thermotolerant yeast mutant

The 26th Symposium of Young Asian Biological Engineers’ Community 2021.11
緑藻と枯草菌との共培養による増殖特性の変化 Domestic conference

大山遥行，山田亮祐，松本拓也，荻野博康

第73回日本生物工学会大会 2021.10

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バイオアルカンの生産性改善を指向した大腸菌の代謝改変 Domestic conference

浅野紘輝，松本拓也，山田亮祐，荻野博康

化学工学会第52回秋季大会 2021.09

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バイオアルカンの生産性改善を指向した大腸菌の代謝改変

浅野紘輝, 松本拓也, 山田亮祐, 荻野博康

化学工学会第52回秋季大会 2021.09

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大腸菌によるレアメタルイオンの還元および吸着に関する遺伝子の探索 Domestic conference

鬼頭和也，松本拓也，山田亮祐，荻野博康

化学工学会第86年会 2021.03

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酵母を用いた酵素のミトコンドリア局在化による有用物質生産 Domestic conference

大谷孝，松本拓也，山田亮祐，荻野博康

化学工学会第86年会 2021.03

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大腸菌によるレアメタルイオンの還元および吸着に関する遺伝子の探索

鬼頭和也, 松本拓也, 山田亮祐, 荻野博康

化学工学会第86年会 2021.03
酵母を用いた酵素のミトコンドリア局在化による有用物質生産

大谷孝, 松本拓也, 山田亮祐, 荻野博康

化学工学会第86年会 2021.03

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ゲノム進化法によって創製した熱耐性酵母の遺伝子発現量解析

三ツ井良輔, 山田亮祐, 松本拓也, 吉原静恵, 徳本勇人, 荻野博康

極限環境生物学会2020年度 (第21回)年会 2020.10
酵母を用いた酵素のミトコンドリア局在化によるβ-カロテン高効率生産

大澤知己, 松本拓也, 山田亮祐, 荻野博康

化学工学会第85年会 2020.03
セルロース系バイオマスからのメバロン酸の生産を指向した大腸菌の創生

濱田直樹, 松本拓也, 山田亮祐, 荻野博康

化学工学会第85年会 2020.03
Thermomyces lanuginosus由来リパーゼへの有機溶媒耐性付与

松本拓也, 野上洋平, 山田亮祐, 荻野博康

極限環境生物学会第20回年会 2019.11

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Thermomyces lanuginosus由来リパーゼへの有機溶媒耐性付与

松本拓也, 野上洋平, 山田亮祐, 荻野博康

酵素工学研究会第82回講演会 2019.11

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微生物を触媒とした酸化還元反応の利用

松本拓也

第7回TT-netワークショップ研究発表(大阪府立大学) 2019.10
Construction and analysis of engineered D-lactic acid tolerant Saccharomyces cerevisiae

三ツ井良輔, 山田亮祐, 松本拓也, 荻野博康

18th Asian Pacific Confederation of Chemical Engineering Congress 2019.09
Production of bioalkanes using engineered Escherichia coli

濱志緒里, 松本拓也, 山田亮祐, 荻野博康

18th Asian Pacific Confederation of Chemical Engineering Congress 2019.09
Modulation of the mevalonate pathway in yeast for efficient patchoulol production by global metabolic engineering

山田亮祐, 西川利留, 三ツ井良輔, 松本拓也, 荻野博康

18th Asian Pacific Confederation of Chemical Engineering Congress 2019.09
Improvement of 2,3-butandiol tolerance in yeast by a novel mutagenesis strategy

溝端明日香, 三ツ井良輔, 山田亮祐, 松本拓也, 荻野博康

18th Asian Pacific Confederation of Chemical Engineering Congress 2019.09
The effect of single gene knockout on mevalonate production by Escherichia coli

松本拓也, 濱田直樹, 田中勉, 荻野博康

18th Asian Pacific Confederation of Chemical Engineering Congress 2019.09

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The improvement of organic solvent-tolerant lipase from Thermomyces lanuginosus

松本拓也, 野上洋平, 山田亮祐, 荻野博康

18th Asian Pacific Confederation of Chemical Engineering Congress 2019.09

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The effect of gene knockout on mevalonate production or electricity generation by Escherichia coli

松本拓也, 濱田直樹, 松井琢人, 田中勉, 荻野博康

Biotrans2019 2019.07

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Presentation type：Poster presentation
Harnessing the sortase A-mediated peptide ligation Invited

2019.06

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Presentation type：Oral presentation (invited, special)
Thermomyces lanuginosus由来リパーゼへの有機溶媒耐性付与

松本拓也, 野上洋平, 山田亮祐, 荻野博康

化学工学会第84年会 2019.03

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酵母細胞内におけるタンパク質の部位特異的連結技術

谷口輝, 松本拓也, 山田亮祐, 荻野博康

酵素工学研究会第90回講演会