Updated on 2024/02/16

写真a

 
Miura Natsuko
 
Organization
Graduate School of Agriculture Department of Applied Biological Chemistry Associate Professor
School of Agriculture Department of Applied Biological Chemistry
Title
Associate Professor
Affiliation
Institute of Agriculture
Contact information
メールアドレス
Affiliation campus
Nakamozu Campus

Position

  • Graduate School of Agriculture Department of Applied Biological Chemistry 

    Associate Professor  2023.04 - Now

  • Graduate School of Agriculture Department of Applied Biological Chemistry 

    Assistant Professor  2022.04 - 2023.03

  • School of Agriculture Department of Applied Biological Chemistry 

    Associate Professor  2023.04 - Now

  • School of Agriculture Department of Applied Biological Chemistry 

    Assistant Professor  2022.04 - 2023.03

Degree

  • Ph.D. （ Kyoto University ） (   Kyoto University )

Research Areas

  • Life Science / Applied biochemistry

  • Life Science / Applied microbiology

  • Life Science / Molecular biology

Research Interests

  • Hypoxia

  • Glycolytic enzymes

  • META body

  • G body

  • Coral-associated bacteria

  • Saccharomyces cerevisiae

  • Microbial Screening

  • Bioproduction

  • Moonlighting proteins

Research subject summary

  • 代謝酵素の局在変化による代謝調節機構の解明と利用

  • サンゴ共在細菌の機能解明およびその利用

Professional Memberships

  • Brewing Sciety of Japan

    2023.10 - Now

  • Quantum Life Science Society

    2020.08 - Now

  • マリンバイオテクノロジー学会　

    2019.04 - Now

  • Japanese Society of Enzyme Engineering

    2018.04 - Now

  • The Society for Biotechnology, Japan

    2011.03 - Now

  • JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY

    2010.04 - Now

  • THE MOLECULAR BIOLOGY SOCIETY OF JAPAN

    2010.01 - Now

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Awards

  • Enzyme Engineering Encouragement Award

    Natsuko Miura

    2023.04   Japanese Society of Enzyme Engineering  

  • JSBBA Award for Young Women Scientists

    Natsuko Miura

    2023.03   Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA)  

  • Marine Biotechnology Paper Award

    Ruriko Kitamura, Natsuko Miura, Michihiro Ito, Toshiyuki Takagi, Hideyuki Yamashiro, Yumi Nishikawa, Yuna Nishimura, Keita Kobayashi, Michihiko Kataoka

    2022.05   The Japanese Society of Marine Biotechnology   Specific Detection of Coral‑Associated Ruegeria, a Potential Probiotic Bacterium, in Corals and Subtropical Seawater

  • The 9th New Chemical Technology Research Encouragement Award

    Natsuko Miura

    2020.10   Japan Association for Chemical Innovation  

  • Education Encouragement Award

    Natsuko Miura

    2020.03   Faculty of Liberal Arts and Sciences, Osaka Prefecture University  

  • 第 20 回 酵素応用シンポジウム 研究奨励賞

    2019.06   一般財団法人 天野エンザイム科学技術振興財団  

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    Country：Japan

  • Marie Curie Award

    2015.09   Radiation Research Society  

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Job Career (off-campus)

  • Osaka University   Institute for Protein Research   Research Fellow

    2023.04 - Now

  • 量子科学技術研究開発機構 量子生命・医学部門   量子生命科学研究所 量子超偏極MRIグループ   協力研究員

    2022.04 - Now

Papers

  • Characterization of carotenoid-producing Muricauda sp. strains isolated from the reef-building coral Galaxea fascicularis and subtropical seawater

    Funa Endo, Keisuke Motone, Kenji Kai, Tomoya Kitakaze, Yumi Nishikawa, Yuna Nishimura, Ruriko Kitamura, Toshiyuki Takagi, Michihiro Ito, Natsuko Miura, Michihiko Kataoka

    bioRxiv   2023.12

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    Authorship：Corresponding author   Kind of work：Joint Work  

    ABSTRACT

    Bacterial symbionts in corals and coral-associated zooxanthellae are attracting increasing attention as potential probiotics. Some members of the family Flavobacteriaceae are zooxanthellae-associated bacteria, which are known to protect zooxanthellae from stresses such as heat and light by producing carotenoids that reduce reactive oxygen species production. This study characterized two Flavobacteriaceae bacteria,Muricaudasp. strains ORYM1 (NBRC115792) and ORYM2 (NBRC115793), isolated from the reef-building coralGalaxea fascicularisand its surrounding seawater in Okinawa, Japan, respectively. TheMuricaudasp. strain ORYM2 was isolated from subtropical seawater, and carotenoid production was assessed using ORYM2 as well as ORYM1, which was previously isolated fromG. fascicularis. De novogenome sequencing revealed that both strains contain complete sets of zeaxanthin biosynthesis genes, similar to those found in otherMuricaudaspp. Thin-layer chromatography and high-performance liquid chromatography analyses demonstrated that ORYM1 and ORYM2 produce several carotenoids. The bacterial strains and carotenoids identified in this study provide insights into the biological roles of zooxanthellae-associated bacteria in protecting zooxanthellae and reef-building corals from environmental stresses.

    Statements and declarations

    Competing interests: The authors declare no competing interests.

    DOI： 10.1101/2023.12.12.571358

  • Deciphering mechanisms of UV filter- and temperature-induced bleaching in the coral Acropora tenuis, using ecotoxicogenomics

    Sakiko Nishioka, Kaede Miyata, Yasuaki Inoue, Kako Aoyama, Yuki Yoshioka, Natsuko Miura, Masayuki Yamane, Hiroshi Honda, Toshiyuki Takagi

    bioRxiv   2023.11

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    Kind of work：Joint Work  

    Coral reefs are at risk of bleaching due to various environmental and anthropogenic stressors such as global warming and chemical pollutants. However, there is little understanding of stressor-specific mechanisms that cause coral bleaching. Therefore, conducting accurate ecotoxicological risk assessments and deciphering modes of action of potentially deleterious ultraviolet (UV) filters (sunscreen compounds) are crucial issues. In this study, we evaluated the toxicity and bleaching effect of benzophenone-3 (BP-3), which is widely used in sunscreen products, on the reef-building coral Acropora tenuis. Furthermore, to understand differences in UV filter- and temperature-induced bleaching, a comparative ecotoxicogenomic approach using RNA-seq was integrated into a toxicity test to clarify differences in gene expression changes induced by BP-3 and heat stress (31℃). The lethal concentration 50% (LC50) was calculated as 3.9 mg/L, indicating that emission level of BP-3 was properly controlled based on the risk assessment. Differentially expressed genes related to oxidative stress and extracellular matrix organization were involved in coral responses to both BP-3 and heat stress, but their patterns differed. Whereas immune and heat-shock responses were activated in response to heat stress, activation of a drug metabolism pathway and several signal transduction pathways were identified in BP-3 treatment groups. Our study enhances understanding of stress responses in corals induced by UV filters and thermal stress. Using potential gene markers identified in this study for eco-epidemiological surveys of stressed corals, we urgently need to develop effective countermeasures.

    DOI： 10.1101/2023.11.05.564681

  • Large-scale preparation of yeast strains expressing condensates derived from a glycolytic enzyme via controlled dissolved oxygen levels under hypoxia. Reviewed

    Yuki Murata, Reina Hirayama, Natsuko Miura, Michihiko Kataoka

    Letters in applied microbiology   76 ( 8 )   2023.08（ ISSN:0266-8254 ）

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    Authorship：Corresponding author   Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    Under hypoxia, Saccharomyces cerevisiae forms cytoplasmic condensates composed of proteins, including glycolytic enzymes, that are thought to regulate cellular metabolism. However, the hypoxic conditions required for the condensate formation remain unclear. In this study, we developed a 300-mL scale culture method to produce condensate-forming cells by precisely controlling the dissolved oxygen (DO) level in the media. Using enolase as a model, a foci formation rate of more than 50% was achieved at ∼0.1% DO, and the results showed that the DO level affected the foci formation rate. The foci formation rates of the previously reported foci-deficient strains and strains with single amino acid substitutions in the endogenous enolase were examined, and the effect of these amino acid substitutions on glucose consumption and ethanol and glycerol production under hypoxia were evaluated. The results of this study contribute to the investigation of the mechanisms that regulate biomacromolecular condensates under hypoxia.

    DOI： 10.1093/lambio/ovad095

    PubMed

  • Foci-forming regions of pyruvate kinase and enolase at the molecular surface incorporate proteins into yeast cytoplasmic metabolic enzymes transiently assembling (META) bodies. Reviewed

    Ryotaro Utsumi, Yuki Murata, Sayoko Ito-Harashima, Misaki Akai, Natsuko Miura, Kouichi Kuroda, Mitsuyoshi Ueda, Michihiko Kataoka

    PLOS ONE   18 ( 4 )   e0283002   2023.04

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    Authorship：Corresponding author   Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Spatial reorganization of metabolic enzymes to form the "metabolic enzymes transiently assembling (META) body" is increasingly recognized as a mechanism contributing to regulation of cellular metabolism in response to environmental changes. A number of META body-forming enzymes, including enolase (Eno2p) and phosphofructokinase, have been shown to contain condensate-forming regions. However, whether all META body-forming enzymes have condensate-forming regions or whether enzymes have multiple condensate-forming regions remains unknown. The condensate-forming regions of META body-forming enzymes have potential utility in the creation of artificial intracellular enzyme assemblies. In the present study, the whole sequence of yeast pyruvate kinase (Cdc19p) was searched for condensate-forming regions. Four peptide fragments comprising 27-42 amino acids were found to form condensates. Together with the fragment previously identified from Eno2p, these peptide regions were collectively termed "META body-forming sequences (METAfos)." METAfos-tagged yeast alcohol dehydrogenase (Adh1p) was found to co-localize with META bodies formed by endogenous Cdc19p under hypoxic conditions. The effect of Adh1p co-localization with META bodies on cell metabolism was further evaluated. Expression of Adh1p fused with a METAfos-tag increased production of ethanol compared to acetic acid, indicating that spatial reorganization of metabolic enzymes affects cell metabolism. These results contribute to understanding of the mechanisms and biological roles of META body formation.

    DOI： 10.1371/journal.pone.0283002

    PubMed

  • Mutualistic Interactions between Dinoflagellates and Pigmented Bacteria Mitigate Environmental Stress. Reviewed

    Toshiyuki Takagi, Kako Aoyama, Keisuke Motone, Shunsuke Aburaya, Hideyuki Yamashiro, Natsuko Miura, Koji Inoue

    Microbiology spectrum   11 ( 1 )   e0246422   2023.01

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Scleractinian corals form symbiotic relationships with a variety of microorganisms, including endosymbiotic dinoflagellates of the family Symbiodiniaceae, and with bacteria, which are collectively termed coral holobionts. Interactions between hosts and their symbionts are critical to the physiological status of corals. Coral-microorganism interactions have been studied extensively, but dinoflagellate-bacterial interactions remain largely unexplored. Here, we developed a microbiome manipulation method employing KAS-antibiotic treatment (kanamycin, ampicillin, and streptomycin) to favor pigmented bacteria residing on cultured Cladocopium and Durusdinium, major endosymbionts of corals, and isolated several carotenoid-producing bacteria from cell surfaces of the microalgae. Following KAS-antibiotic treatment of Cladocopium sp. strain NIES-4077, pigmented bacteria increased 8-fold based on colony-forming assays from the parental strain, and 100% of bacterial sequences retrieved through 16S rRNA amplicon sequencing were affiliated with the genus Maribacter. Microbiome manipulation enabled host microalgae to maintain higher maximum quantum yield of photosystem II (variable fluorescence divided by maximum fluorescence [Fv/Fm]) under light-stress conditions, compared to the parental strain. Furthermore, by combining culture-dependent and -independent techniques, we demonstrated that species of the family Symbiodiniaceae and pigmented bacteria form strong interactions. Dinoflagellates protected bacteria from antibiotics, while pigmented bacteria protected microalgal cells from light stress via carotenoid production. Here, we describe for the first time a symbiotic relationship in which dinoflagellates and bacteria mutually reduce environmental stress. Investigations of microalgal-bacterial interactions further document bacterial contributions to coral holobionts and may facilitate development of novel techniques for microbiome-mediated coral reef conservation. IMPORTANCE Coral reefs cover less than 0.1% of the ocean floor, but about 25% of all marine species depend on coral reefs at some point in their life cycles. However, rising ocean temperatures associated with global climate change are a serious threat to coral reefs, causing dysfunction of the photosynthetic apparatus of endosymbiotic microalgae of corals, and overproducing reactive oxygen species harmful to corals. We manipulated the microbiome using an antibiotic treatment to favor pigmented bacteria, enabling their symbiotic microalgal partners to maintain higher photosynthetic function under insolation stress. Furthermore, we investigated mechanisms underlying microalgal-bacterial interactions, describing for the first time a symbiotic relationship in which the two symbionts mutually reduce environmental stress. Our findings extend current insights about microalgal-bacterial interactions, enabling better understanding of bacterial contributions to coral holobionts under stressful conditions and offering hope of reducing the adverse impacts of global warming on coral reefs.

    DOI： 10.1128/spectrum.02464-22

    PubMed

  • Condensate formation by metabolic enzymes in Saccharomyces cerevisiae. Invited Reviewed

    Natsuko Miura

    Microorganisms   10 ( 2 )   232   2022.01

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    Authorship：Lead author,　Last author,　Corresponding author   Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Repository URL： http://hdl.handle.net/10466/00017827

  • Peptide barcoding for one-pot evaluation of sequence-function relationships of nanobodies. Reviewed

    Yusei Matsuzaki, Wataru Aoki, Takumi Miyazaki, Shunsuke Aburaya, Yuta Ohtani, Kaho Kajiwara, Naoki Koike, Hiroyoshi Minakuchi, Natsuko Miura, Tetsuya Kadonosono, Mitsuyoshi Ueda

    Scientific Reports 雑誌   11 ( 1 )   21516 - 21516   2021.11

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    Kind of work：Joint Work  

  • Simple Esterification of [1-13C]-Alpha-Ketoglutarate Enhances Membrane Permeability and Allows for Noninvasive Tracing of Glutamate and Glutamine Production. Reviewed

    Jenna E AbuSalim, Kazutoshi Yamamoto, Natsuko Miura, Burchelle Blackman, Jeffrey R Brender, Chandrasekhar Mushti, Tomohiro Seki, Kevin A Camphausen, Rolf E Swenson, Murali C Krishna, Aparna H Kesarwala

    ACS Chemical Biology 雑誌   16 ( 11 )   2144 - 2150   2021.09

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    Kind of work：Joint Work  

  • Specific Detection of Coral-Associated Ruegeria, a Potential Probiotic Bacterium, in Corals and Subtropical Seawater. Reviewed

    Ruriko Kitamura, Natsuko Miura, Michihiro Ito, Toshiyuki Takagi, Hideyuki Yamashiro, Yumi Nishikawa, Yuna Nishimura, Keita Kobayashi, Michihiko Kataoka

    Marine Biotechnology 雑誌   23   2021.07

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    Kind of work：Joint Work  

  • Synthesis of [1-13 C-5-12 C]-alpha-ketoglutarate enables noninvasive detection of 2-hydroxyglutarate. Reviewed

    Natsuko Miura, Chandrasekhar Mushti, Deepak Sail, Jenna E AbuSalim, Kazutoshi Yamamoto, Jeffrey R Brender, Tomohiro Seki, Deyaa I AbuSalim, Shingo Matsumoto, Kevin A Camphausen, Murali C Krishna, Rolf E Swenson, Aparna H Kesarwala

    NMR in biomedicine 雑誌   34 ( 11 )   2021.07

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    Kind of work：Joint Work  

  • Small-scale hypoxic cultures for monitoring the spatial reorganization of glycolytic enzymes in Saccharomyces cerevisiae. Reviewed

    Yuki Yoshimura, Reina Hirayama, Natsuko Miura, Ryotaro Utsumi, Kouichi Kuroda, Mitsuyoshi Ueda, Michihiko Kataoka

    Cell biology international 雑誌   45 ( 8 )   1776 - 1783   2021.04

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    Kind of work：Joint Work  

  • Design of novel primer sets for easy detection of Ruegeria species from seawater. Reviewed

    Kitamura R, Miura N*, Okada K, Motone K, Takagi T, Ueda M, Kataoka M.

    Bioscience, Biotechnology, and Biochemistry. 雑誌   84   854 - 864   2020

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    Kind of work：Joint Work  

  • A zeaxanthin-producing bacterium isolated from the algal phycosphere protects coral endosymbionts from environmental stress. Reviewed

    Motone K, Takagi T, Aburaya S, Miura N, Aoki W, Ueda M.

    mBio 雑誌   11   e01019 - 19   2020

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    Kind of work：Joint Work  

  • 選択的反応モニタリング質量分析のための1mの長さの一体型カラムの評価(Evaluation of meter-long monolithic columns for selected reaction monitoring mass spectrometry) Reviewed International coauthorship

    Ohtani Yuta, Aburaya Shunsuke, Minakuchi Hiroyoshi, Miura Natsuko, Aoki Wataru, Ueda Mitsuyoshi

    Journal of Bioscience and Bioengineering   128 ( 3 )   379 - 383   2019.09（ ISSN:1389-1723 ）

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    Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    内径が小さい1mの長さの一体型カラムを開発し、プロテオミクス的検出感度改善のための選択的反応モニタリング(SRM)に基づくプロテオミクスに使用した。ウシ血清アルブミントリプシン分解物を至適なSRM法で分析し、それぞれの一体型カラムでの分離効率と検出感度を評価した。その結果、ピークキャパシティーは約1.8倍、ピーク面積は約2.3倍に上昇した。小さな内径のカラムなので流速は低下したが、ピーク面積の再現性は維持された。以上より、内径の小さい1mの長さの一体型カラムは、SRMによるプロテオミクスに役立ちうると考えられた。

  • Domain swapping of complementarity-determining region in nanobodies produced by Pichia pastoris. Reviewed

    Miura N, Miyamoto K, Ohtani Y, Yaginuma K, Aburaya S, Kitagawa Y, Aoki W, Ueda M

    AMB Express 雑誌   9 ( 1 )   2019.07

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    Kind of work：Joint Work  

  • High-throughput identification of peptide agonists against GPCRs by co-culture of mammalian reporter cells and peptide-secreting yeast cells using droplet microfluidics. Reviewed

    Yaginuma K, Aoki W, Miura N, Ohtani Y, Aburaya S, Kogawa M, Nishikawa Y, Hosokawa M, Takeyama H, Ueda M

    Scientific reports 雑誌   9 ( 1 )   2019.07

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    Kind of work：Joint Work  

  • Evaluation of meter-long monolithic columns for selected reaction monitoring mass spectrometry. Reviewed

    Ohtani Y, Aburaya S, Minakuchi H, Miura N, Aoki W, Ueda M

    Journal of bioscience and bioengineering 雑誌   128 ( 3 )   379 - 383   2019.04

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    Kind of work：Joint Work  

  • Peptide barcoding for establishment of new types of genotype-phenotype linkages. Reviewed

    Miyamoto K, Aoki W, Ohtani Y, Miura N, Aburaya S, Matsuzaki Y, Kajiwara K, Kitagawa Y, Ueda M.

    PLoS One 雑誌   14 ( 4 )   2019.04

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    Kind of work：Joint Work  

  • Ruegeria sp. Strains Isolated from the Reef-Building Coral Galaxea fascicularis Inhibit Growth of the Temperature-Dependent Pathogen Vibrio coralliilyticus Reviewed

    Miura N, Motone K, Takagi T, Aburaya S, Watanabe S, Aoki W, Ueda M

    Marine Biotechnology 雑誌 Springer Nature   2018.09

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    Kind of work：Joint Work  

  • Protection of Coral Larvae from Thermally Induced Oxidative Stress by Redox Nanoparticles. Reviewed

    Motone K, Takagi T, Aburaya S, Aoki W, Miura N, Minakuchi H, Takeyama H, Nagasaki Y, Shinzato C, Ueda M.

    Marine Biotechnology 雑誌   20   542 - 548   2018.08

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    Kind of work：Joint Work  

  • Evaluation of Unconventional Protein Secretion by Saccharomyces cerevisiae and other Fungi Reviewed

    Miura N, Ueda M

    cells 雑誌 MDPI AG, Basel, Switzerland   7 ( 9 )   2018.08

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    Kind of work：Joint Work  

  • Three-Dimensional Alginate Hydrogels for Radiobiological and Metabolic Studies of Cancer Cells Reviewed

    Read GH, Miura N, Carter JL, Kines KT, Devasahayam N, Cheng JY, Camphausen KA, Krishna MC, Kesarwala AH.

    Colloids and Surfaces B: Biointerfaces 雑誌   171   197 - 204   2018.06

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    Kind of work：Joint Work  

  • Enzyme Evolution.

    Miura N

    Yeast Cell Surface Engineering – Biological Mechanism and Its Practical Applications. 著書 Springer Nature   2018

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    Kind of work：Single Work  

  • Reconstruction of Thermotolerant Yeast by One-Point Mutation Identified through Whole-Genome Analyses of Adaptively-Evolved Strains. Reviewed

    Satomura A, Miura N, Kuroda K, Ueda M.

    Scientific Reports 雑誌   6   2016.03

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    Kind of work：Joint Work  

  • Enzyme Evolution by Yeast Cell Surface Engineering.

    Miura N, Kuroda K, Ueda M.

    Methods in Molecular Biology 著書   1319   2015

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    Kind of work：Joint Work  

  • Evaluation of Unconventional Protein Secretion in Saccharomyces cerevisiae.

    Miura N, Ueda M.

    Methods in Molecular Biology 著書   1270   2015

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    Kind of work：Joint Work  

  • Single-cell Heterogeneity in Suppression of PC12 Differentiation by Direct Microinjection of a Differentiation Inhibitor, U0126. Reviewed

    Chung J, Miura N, Ito A, Sawada M, Nishikawa S, Kuroda K, Ueda M.

    Cell Biology International 雑誌   38 ( 10 )   2014

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    Kind of work：Joint Work  

  • Application of Cell Surface Engineered Yeast for the Development of Drugs Against Influenza Virus Infection.

    Yamada J, Miura N, Shigemori T, Kuroda K, Ueda M.

    Influenza and RNA Viruses: Emergence, Classification and Management. 著書 Nova Science Publishers   2014

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    Kind of work：Joint Work  

  • Spatial Reorganization of Saccharomyces cerevisiae Enolase To Alter Carbon Metabolism under Hypoxia. Reviewed

    Miura N, Shinohara M, Tatsukami Y, Sato Y, Morisaka H, Kuroda K, Ueda M.

    Eukaryotic Cell 雑誌   12 ( 8 )   2013

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    Kind of work：Joint Work  

  • Mutant Firefly Luciferases with Improved Specific Activity and dATP Discrimination Constructed by Yeast Cell Surface Engineering. Reviewed

    Fushimi T, Miura N, Shintani H, Tsunoda H, Kuroda K, Ueda M.

    Applied Microbiology and Biotechnology 雑誌   97 ( 9 )   2013

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    Kind of work：Joint Work  

  • Acquisition of Thermotolerant Yeast Saccharomyces cerevisiae by Breeding via Stepwise Adaptation. Reviewed

    Satomura A, Katsuyama Y, Miura N, Kuroda K, Tomio A, Bamba T, Fukusaki E, Ueda M.

    Biotechnology Progress 雑誌   29   2013

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    Kind of work：Joint Work  

  • Construction of a Convenient System for Easily Screening Inhibitors of Mutated Influenza Virus Neuraminidases. Reviewed

    Shigemori T, Nagayama M, Yamada J, Miura N, Yongkiettrakul S, Kuroda K, Katsuragi T, Ueda M.

    FEBS Open Bio 雑誌   3   2013

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    Kind of work：Joint Work  

  • Tracing Putative Trafficking of the Glycolytic Enzyme Enolase via SNARE-Driven Unconventional Secretion. Reviewed

    Miura N, Kirino A, Endo S, Morisaka H, Kuroda K, Takagi M, Ueda M.

    Eukaryotic Cell 雑誌   11 ( 8 )   2012

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    Kind of work：Joint Work  

  • Design of a Novel Antimicrobial Peptide Activated by Virulent Proteases. Reviewed

    Aoki W, Kitahara N, Miura N, Morisaka H, Kuroda K, Ueda M.

    Chemical Biology & Drug Design 雑誌   80 ( 5 )   2012

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    Kind of work：Joint Work  

  • Candida albicans Possesses Sap7 as a Pepstatin A-Insensitive Secreted Aspartic Protease. Reviewed

    Aoki W, Kitahara N, Miura N, Morisaka H, Yamamoto Y, Kuroda K, Ueda M.

    PLoS ONE 雑誌   7   2012

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    Kind of work：Joint Work  

  • Profiling of Adhesive Properties of the Agglutinin-like Sequence (ALS) Protein Family, a Virulent Attribute of Candida albicans. Reviewed

    Aoki W, Kitahara N, Miura N, Morisaka H, Kuroda K, Ueda M.

    FEMS Immunology and Medical Microbiology 雑誌   65   2012

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    Kind of work：Joint Work  

  • Comprehensive Characterization of Secreted Aspartic Proteases Encoded by a Virulence Gene Family in Candida albicans. Reviewed

    Aoki W, Kitahara N, Miura N, Morisaka H, Yamamoto Y, Kuroda K, Ueda M.

    Journal of Biochemistry 雑誌   150 ( 4 )   2011

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    Kind of work：Joint Work  

  • Cell-Surface Modification of Non-GMO without Chemical Treatment by Novel GMO-Coupled and-Separated Cocultivation Method. Reviewed

    Miura N, Aoki W, Tokumoto N, Kuroda K, Ueda M.

    Applied Microbiology and Biotechnology 雑誌   82 ( 2 )   2009

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    Kind of work：Joint Work  

  • Surface Coat Proteins of the Pine Wood Nematode, Bursaphelenchus xylophilus: Profiles of Stage and Isolate-Specific Characters. Reviewed

    Shinya R, Takeuchi Y, Miura N, Kuroda K, Ueda M, Futai K.

    Nematology 雑誌   11   2009

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    Kind of work：Joint Work  

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Books and Other Publications

  • 低酸素条件下で形成する代謝酵素集合体の観察

    三浦夏子（ Role： Contributor ,  加藤昌人・白木賢太郎・中川真一編, フロントランナー直伝 相分離解析プロトコール (pp.135-145)）

    羊土社  2022.07 

  • 酵素工学への展開

    三浦夏子, 片岡道彦（ Role： Joint author）

    シーエムシー出版  2020.04 

  • ムーンライティング酵素の展開

    三浦夏子, 片岡道彦（ Role： Joint author）

    シーエムシー出版  2019.05  （ ISBN:978-4-7813-1411-2 ）

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    Responsible for pages：247-258  

  • Enzyme Evolution

    Miura N（ Role： Sole author）

    Springer Nature  2019  （ ISBN:978-981-13-5867-8 ）

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    Responsible for pages：175-185  

  • 非侵襲的代謝診断の臨床応用（実用化）に向けたビッグデータ活用への期待

    三浦夏子（ Role： Sole author）

    シーエムシー出版  2018.02  （ ISSN: 978-4-7813-1315-3 ）

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    Responsible for pages：66-74  

  • 変異タンパク質およびタンパク質ドメインを用いたshort RNAの回収、分離技術

    三浦夏子, 林絵理, 植田充美（ Role： Joint author）

    シーエムシー出版  2010 

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MISC

  • 【若手研究者が拓くこれからの生物工学(前編)】生体内の代謝を知る・みる・使う Invited

    三浦 夏子

    生物工学会誌   100 ( 5 )   240 - 245   2022.05（ ISSN:0919-3758 ）

  • 解糖系酵素の局在制御とリンクした代謝制御ー集まると仕事が早く進む？ Invited

    三浦夏子

    化学と生物   58 ( 1 )   10 - 12   2020.01

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    Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

  • 低酸素条件下で形成される代謝酵素集合体G-body の発見 Invited International journal

    三浦夏子

    酵素工学研究会 酵素工学ニュース   ( 80 )   5 - 10   2018.10

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  • Coral Symbionts Invited International journal

    Natsuko Miura

    Journal of Bioscience and Bioengineering   96 ( 8 )   2018.08

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  • 金属イオン吸着・回収に向けた細胞表層デザインと吸着分子の創製

    黒田浩一, 三浦夏子

    日本生物工学会誌   2008

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  • バイオマスデザインに向けた植物育種の新発想

    黒田浩一, 三浦夏子, 植田充美

    BIOINDUSTRY   2008

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Presentations

  • Moonlighting glycolytic enzymes in Saccharomyces cerevisiae Invited Domestic conference

    Natsuko Miura

    The 46th Annual Meeting of the Molecular Biology Society of Japan  2023.12 

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    Presentation type：Oral presentation (invited, special)  

  • Liquid-liquid phase separation of enzymes Invited Domestic conference

    Natsuko Miura

    The 46th Annual Meeting of the Molecular Biology Society of Japan  2023.12 

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    Presentation type：Oral presentation (invited, special)  

  • Liquid-liquid phase separation of glycolytic enzymes Invited Domestic conference

    Natsuko Miura

    The 61st Annual Meeting of the BSJ Symposium: The forefront of biophysical research of liquid-liquid phase separation  2023.11 

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    Presentation type：Symposium, workshop panel (public)  

  • Emerging Functions of Coral- and Zooxanthellae-Associated Bacteria Invited Domestic conference

    Natsuko Miura

    2023.09 

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    Presentation type：Oral presentation (invited, special)  

  • Flux regulation in the violacein biosynthesis pathway using enzyme assembly formation Domestic conference

    Rioko Ito, Natsuko Miura, Michihiko Kataoka

    The 75th Annual Meeting of the Society of Biotechnology of Japan  2023.09 

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    Presentation type：Oral presentation (general)  

  • 海洋生分解性プラスチックを分解する酵素PhaZの異種発現系構築 および分解活性測定方法の検討について Domestic conference

    柿沼 亜望, 三浦 夏子, 片岡 道彦

    第75回日本生物工学会大会  2023.09 

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    Presentation type：Oral presentation (general)  

  • 低酸素条件下における代謝酵素群による集合体形成の発見およびその制御機構の解析 Invited Domestic conference

    三浦夏子

    ⽇本農芸化学会関⻄⽀部 第509 回講演会 受賞記念講演  2023.07 

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    Presentation type：Oral presentation (invited, special)  

  • 代謝酵素集合体 G-body の形成を制御する低分子化合物の探索 Domestic conference

    水野有沙, 三浦夏子, 片岡道彦

    生物工学若手研究者の集い（若手会）夏のセミナー2023  2023.06 

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    Presentation type：Poster presentation  

  • 細胞における代謝酵素群の集合体形成に学ぶ Invited

    三浦 夏子

    分子研ExCELLS研究会「生命の分子システムの理解に向けて何を創れば良いか？」  2023.05 

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    Presentation type：Oral presentation (invited, special)  

  • 低酸素条件下における解糖系酵素の集合体形成の発見およびその制御機構の解析. Invited

    三浦夏子

    酵素工学研究会第８９回講演会 2022年度酵素工学奨励賞受賞講演.  2023.04 

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    Presentation type：Oral presentation (invited, special)  

  • METAbody形成タグを用いた酵素集合体の人為的な構築 Invited

    三浦夏子

    学術変革領域研究(A)分子サイバネティクス・超越分子システム合同シンポジウム2023  2023.03 

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    Presentation type：Oral presentation (invited, special)  

  • 低酸素条件下で細胞質に集合体を形成する解糖系酵素 Invited

    三浦 夏子

    第9回タタバイオ分子クラブ  2023.03 

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    Presentation type：Oral presentation (invited, special)  

  • 低酸素条件下における代謝酵素群による集合体形成の発見およびその制御機構の解析. Invited

    三浦夏子

    日本農芸化学会2023年度大会 農芸化学若手女性研究者賞受賞者講演  2023.03 

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    Presentation type：Oral presentation (invited, special)  

  • Exploring the principle of formation of the META body, a condensate formed by a group of central metabolic enzymes in the cytoplasm Invited

    Natsuko Miura

    The 45th Annual Meeting of the Molecular Biology Society of Japan  2022.11 

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    Presentation type：Symposium, workshop panel (nominated)  

  • 解糖系酵素が形成する細胞内集合体とその制御 Invited Domestic conference

    三浦夏子

    第74回日本生物工学会大会シンポジウム「S23 最先端の代謝研究が解き明かす解糖系の深淵—Otto Meyerhof ノーベル賞受賞100周年によせて—」  2022.10 

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    Presentation type：Symposium, workshop panel (nominated)  

  • 分離共培養容器を用いたサンゴ共在細菌間相互作用の実験的再構築

    中島玲, 西川有美, 三浦夏子, 植田充美, 片岡道彦

    ワークショップ「サンゴ礁生態系におけるレドックスエコバイオロジー」  2022.10 

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    Presentation type：Poster presentation  

  • 沖縄県のサンゴと海水から単離されたMuricauda属細菌 ORYM1, ORYM2株の機能探索

    遠藤芙奈, 西川有美, 高木俊幸, 三浦夏子, 片岡道彦

    ワークショップ「サンゴ礁生態系におけるレドックスエコバイオロジー」  2022.10 

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    Presentation type：Poster presentation  

  • 生命を駆動する中心代謝酵素の新たな挙動－酵素集合体形成による可逆的な細胞内代謝区画の形成 Invited

    三浦夏子

    第73回コロイド及び界面化学討論会  2022.09 

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    Presentation type：Symposium, workshop panel (nominated)  

  • 低酸素条件下での集合体形成による細胞の代謝調節 Invited

    三浦夏子

    2022年日本農芸化学会関西支部大会（第522回講演会）  2022.09 

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    Presentation type：Oral presentation (invited, special)  

  • 低酸素条件下で形成される代謝酵素集合体−細胞内の新たな代謝調節機構？ Invited

    三浦夏子

    日本環境変異原ゲノム学会変異機構研究会・第33回夏の学校  2022.09 

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    Presentation type：Oral presentation (invited, special)  

  • G-body形成に最適な酵母低酸素培養系の評価

    村田有紀, 吉村柚紀, 平山伶奈, 内海亮太郎, 伊藤里緒子, 三浦夏子, 片岡道彦

    日本農芸化学会関西支部 第521回講演会  2022.07 

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    Presentation type：Oral presentation (general)  

  • 沖縄県のサンゴと海水から単離されたMuricauda属細菌 ORYM1, ORYM2株の機能探索

    遠藤芙奈, 西川有美, 高木俊幸, 三浦夏子, 片岡 道彦

    第22回マリンバイオテクノロジー学会大会  2022.05 

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    Presentation type：Poster presentation  

  • Specific Detection of Coral‑Associated Ruegeria, a Potential Probiotic Bacterium, in Corals and Subtropical Seawater Invited

    北村瑠璃子, 三浦夏子, 伊藤通浩, 高木俊幸, 山城秀之, 西川有美, 西村優菜, 小林慶太, 片岡道彦

    第22回マリンバイオテクノロジー学会大会 論文賞受賞講演  2022.05 

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    Presentation type：Oral presentation (invited, special)  

  • 簡便にG-body形成が可能な低酸素培養系の確立

    伊藤里緒子, 村田有紀, 吉村柚紀, 平山伶奈, 内海亮太郎, 三浦夏子, 片岡道彦

    生物工学若手研究者の集い 夏のオンラインセミナー2022  2022.05 

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    Presentation type：Oral presentation (general)  

  • 「生命の不思議:細胞とMETA body 」 〜 酵素集合体形成による細胞の代謝制御 〜 Invited

    三浦夏子

    MPUFセミナー  2022.05 

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    Presentation type：Public lecture, seminar, tutorial, course, or other speech  

  • 細胞質内で形成される解糖系酵素集合体G-bodyとその制御 Invited

    三浦夏子

    JT医薬総合研究所講演会「相分離が支配する酵素反応系」  2022.04 

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    Presentation type：Symposium, workshop panel (nominated)  

  • 簡便に G body 形成が可能な低酸素培養系の確立

    伊藤里緒子, 村田有紀, 吉村柚紀, 平山伶奈, 内海亮太郎, 三浦夏子, 片岡道彦

    酵素工学研究会第８７回講演会  2022.04 

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    Presentation type：Poster presentation  

  • Development of in vitro hyperpolarized 13C NMR method for measurement of enzymatic reactions under hypoxia Invited

    2nd Metabolic Imaging Workshop in Gifu  2022.03 

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    Presentation type：Public lecture, seminar, tutorial, course, or other speech  

  • Metabolic regulation through spatial reorganization of metabolic enzymes Invited Domestic conference

    Natsuko Miura

    The 2022 Annual Meeting of The Japan Society for Bioscience, Biotechnology and Agrochemistry  2022.03 

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    Presentation type：Symposium, workshop panel (nominated)  

  • 代謝酵素群の集合体形成による細胞内代謝の調節 Invited

    三浦夏子

    META body研究会 第1回セミナー  2022.03 

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    Presentation type：Public lecture, seminar, tutorial, course, or other speech  

  • 生体内の代謝反応をリアルタイムにみる

    三浦夏子

    第3 回ACT-X 生命と化学 勉強会  2022.02 

  • Condensate Formation by Glycolytic Enzymes To Alter Carbon Metabolism under Hypoxia. Invited

    Natsuko Miura

    RIKEN CSRS seminar  2022.01 

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    Presentation type：Public lecture, seminar, tutorial, course, or other speech  

  • 低酸素環境下における酵素反応のin silico/in vitroリアルタイム測定系構築に関する検討 Domestic conference

    三浦夏子

    量子生命科学先端フォーラム 2021冬の研究会  2021.12 

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    Presentation type：Poster presentation  

  • 低酸素条件下における代謝酵素集合体“G-body"形成メカニズムの解明と制御に向けて Invited Domestic conference

    三浦夏子

    第44 回⽇本分⼦⽣物学会年会ワークショップ1PWS2-07「液-液相分離の酵素連続反応に対する効果」  2021.12 

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    Presentation type：Poster presentation  

  • 低酸素条件下における代謝酵素集合体“G-body”の形成 Invited Domestic conference

    三浦 夏子

    第94回日本生化学会大会シンポジウム3S11a「相分離メガネを通して⾒た酵素の機能」  2021.11 

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    Presentation type：Poster presentation  

  • ジャーファーメンターでの低酸素培養におけるG-body形成のための通気条件の検討 Domestic conference

    村田有紀, 三浦夏子, 片岡道彦

    2021年度生物工学若手研究者の集いオンラインセミナー  2021.07 

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    Presentation type：Oral presentation (general)  

  • カリケアミシン添加によるG-body形成への影響 Domestic conference

    園田啓吾, 三浦夏子, 片岡道彦

    生物工学若手研究者の集い 夏のオンラインセミナー2021  2021.07 

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    Presentation type：Oral presentation (general)  

  • 海洋由来の生分解性プラスチック分解菌の探索とその特性評価 Domestic conference

    小林 慶太, 三浦 夏子, 片岡 道彦

    第21回マリンバイオテクノロジー学会大会   2021.05 

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    Presentation type：Poster presentation  

  • Regulation of G-body formation by Saccharomyces cerevisiae enolase International conference

    Miura N, Hirayama R, Yoshimura Y, Utsumi R, Ueda M, Kataoka M

    Oxidative Stress and Disease Gordon Research Conference  2019.03 

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    Presentation type：Poster presentation  

  • アザミサンゴGalaxea fascicularisから単離したRuegeria属細菌の解析 Domestic conference

    三浦夏子, 岡田圭以子, 元根啓佑, 北村瑠璃子, 高木俊幸, 植田充美, 片岡道彦

    日本農芸化学会2019年度大会  2019.03 

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    Presentation type：Poster presentation  

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Grant-in-Aid for Scientific Research

  • 低酸素下で形成される動的な代謝酵素集合体METAbody制御仮説の構築

    Grant-in-Aid for Scientific Research(B)  2025

  • Bottom-up creation of cell-free molecular systems: surpassing nature

    Grant-in-Aid for Transformative Research Areas (A)  2024

  • 低酸素下で形成される動的な代謝酵素集合体METAbody制御仮説の構築

    Grant-in-Aid for Scientific Research(B)  2024

Charge of on-campus class subject

  • 発酵微生物学実験

    2024   Weekly class   Undergraduate

  • 生命機能化学概論

    2024   Weekly class   Undergraduate

  • 初年次ゼミナール

    2024   Weekly class   Graduate school

  • 生物代謝制御学

    2024   Weekly class   Undergraduate

  • 発酵制御化学特論

    2024   Weekly class   Graduate school

  • 生命機能化学研究プレゼンテーション

    2024   Intensive lecture   Graduate school

  • 生命機能化学特論B

    2024   Weekly class   Graduate school

  • 微生物学実験

    2024   Weekly class   Undergraduate

  • 食品衛生学実習

    2023   Intensive lecture   Undergraduate

  • 生命機能化学概論

    2023   Weekly class   Undergraduate

  • 微生物学実験

    2023   Weekly class   Undergraduate

  • 生物代謝制御学

    2023   Weekly class   Undergraduate

  • 基礎化学実験

    2023   Weekly class   Graduate school

  • 発酵制御化学特論

    2023   Weekly class   Graduate school

  • 生命機能化学研究プレゼンテーション

    2023   Intensive lecture   Graduate school

  • 生命機能化学特論B

    2023   Weekly class   Graduate school

  • 生命機能化学概論

    2022   Weekly class   Undergraduate

  • 生命機能化学研究プレゼンテーション

    2022   Intensive lecture   Graduate school

  • 発酵制御化学特論

    2022   Weekly class   Graduate school

  • 微生物学実験

    2022   Weekly class   Undergraduate

  • 生物代謝制御学

    2022   Weekly class   Undergraduate

  • Laboratory : Microbiology

    2021   Practical Training  

  • General Principles of Applied Biological Chemistry

    2021    

  • Metabolic Regulation and Engineering

    2021    

▼display all

Charge of off-campus class subject

  • 生体触媒分子論（オムニバス講義の１回を担当）

    2022.05