Updated on 2026/04/10

写真a

 
Miura Natsuko
 
Organization
Graduate School of Agriculture Department of Applied Biological Chemistry Associate Professor
School of Agriculture Department of Applied Biological Chemistry
Title
Associate Professor
Affiliation
Institute of Agriculture
Contact information
メールアドレス
Affiliation campus
Nakamozu Campus

Position

  • Graduate School of Agriculture Department of Applied Biological Chemistry 

    Associate Professor  2023.04 - Now

  • Graduate School of Agriculture Department of Applied Biological Chemistry 

    Assistant Professor  2022.04 - 2023.03

  • School of Agriculture Department of Applied Biological Chemistry 

    Associate Professor  2023.04 - Now

  • School of Agriculture Department of Applied Biological Chemistry 

    Assistant Professor  2022.04 - 2023.03

Degree

  • Ph.D. （ Kyoto University ） (   Kyoto University )

Research Areas

  • Life Science / Molecular biology

  • Life Science / Applied biochemistry

  • Life Science / Applied microbiology

Research Interests

  • Hypoxia

  • Glycolytic enzymes

  • META body

  • G body

  • Coral-associated bacteria

  • Saccharomyces cerevisiae

  • Microbial Screening

  • Bioproduction

  • Moonlighting proteins

Research subject summary

  • 代謝酵素の局在変化による代謝調節機構の解明と利用

  • サンゴ共在細菌の機能解明およびその利用

Professional Memberships

  • Brewing Sciety of Japan

    2023.10 - Now

  • Quantum Life Science Society

    2020.08 - Now

  • マリンバイオテクノロジー学会　

    2019.04 - Now

  • Japanese Society of Enzyme Engineering

    2018.04 - Now

  • The Society for Biotechnology, Japan

    2011.03 - Now

  • JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY

    2010.04 - Now

  • THE MOLECULAR BIOLOGY SOCIETY OF JAPAN

    2010.01 - Now

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Awards

  • Ohsumi Life Science Research Society Young Scientist Award

    Natsuko Miura

    2025.12   Society for Prolongation of Healthy Life Expectancy  

  • 第4回 生物工学若手賞

    三浦 夏子

    2025.09   公益社団法人日本生物工学会  

  • Bioindustry Research Award

    Natsuko Miura

    2024.10   Japan Bioindustry Association  

  • Enzyme Engineering Encouragement Award

    Natsuko Miura

    2023.04   Japanese Society of Enzyme Engineering  

  • JSBBA Award for Young Women Scientists

    Natsuko Miura

    2023.03   Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA)  

  • Marine Biotechnology Paper Award

    Ruriko Kitamura, Natsuko Miura, Michihiro Ito, Toshiyuki Takagi, Hideyuki Yamashiro, Yumi Nishikawa, Yuna Nishimura, Keita Kobayashi, Michihiko Kataoka

    2022.05   The Japanese Society of Marine Biotechnology   Specific Detection of Coral‑Associated Ruegeria, a Potential Probiotic Bacterium, in Corals and Subtropical Seawater

  • The 9th New Chemical Technology Research Encouragement Award

    Natsuko Miura

    2020.10   Japan Association for Chemical Innovation  

  • Education Encouragement Award

    Natsuko Miura

    2020.03   Faculty of Liberal Arts and Sciences, Osaka Prefecture University  

  • 第 20 回 酵素応用シンポジウム 研究奨励賞

    2019.06   一般財団法人 天野エンザイム科学技術振興財団  

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    Country：Japan

  • Marie Curie Award

    2015.09   Radiation Research Society  

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Job Career (off-campus)

  • 量子科学技術研究開発機構 量子生命・医学部門   量子生命科学研究所 量子超偏極MRI   客員研究員

    2024.03 - Now

  • Osaka University   Institute for Protein Research   Research Fellow

    2023.04 - Now

  • 量子科学技術研究開発機構 量子生命・医学部門   量子生命科学研究所 量子超偏極MRIグループ   協力研究員

    2022.04 - Now

Papers

  • Investigation of the functional hot-spot residues of an enzyme by real-time monitoring of the enzymatic reaction using NMR and computational approaches. Reviewed

    Toshihiko Sugiki, Tomoki Yoshida, Masaki Tsukamoto, Koichiro Miyanishi, Akinori Kagawa, Natsuko Miura, Tomoto Ura, Jun Fukazawa, Yuko Hatanaka, Tsuyoshi Murata, Toshimichi Fujiwara, Masahiro Kitagawa, Yasushi Morita, Kumiko Sakai-Kato, Yoichi Takakusagi, Nobutada Tanaka, Makoto Negoro

    Scientific reports   16 ( 1 )   5896 - 5896   2026.01

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    Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    UNLABELLED: Favipiravir is an anti-influenza prodrug that is metabolized to its phosphoribosylated form, favipiravir-ribofuranosyl-5′-monophosphate (favipiravir-RMP), by human endogenous enzyme hypoxanthine-guanine phosphoribosyltransferase (HGPRT). This enzymatic reaction is the rate-determining step in generating the active form of favipiravir, making it important to understand the molecular mechanisms underlying the HGPRT-catalyzed RMP-modification of favipiravir. However, the pharmacokinetics of this reaction have not been fully elucidated, despite X-ray crystallographic studies on the HGPRT-favipiravir complex. Here, we identified functional hot-spot residues in HGPRT that play important roles in the enzymatic conversion of favipiravir to favipiravir-RMP. Real-time monitoring of the HGPRT reaction via ligand-observed solution NMR experiments, biochemical mutagenesis of HGPRT, and computational calculations and molecular dynamics simulations, allowed us to investigate the free binding energetics and structural properties of the interaction between HGPRT and favipiravir-RMP. This powerful hybrid experimental strategy allows the identification of functional hot-spot residues in the enzyme and provide complementary structural biological information. This approach could be universally applicable to investigating drug-protein interaction modes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1038/s41598-026-35354-3.

    DOI： 10.1038/s41598-026-35354-3

    PubMed

  • Enhanced deoxyviolacein bioproduction via enzyme condensation with a yeast glycolytic enzyme-derived peptide tag. Reviewed

    Rioko Ito, Sayoko Ito-Harashima, Naoko Segawa, Natsuko Miura, Michihiko Kataoka

    Biotechnology progress   42 ( 1 )   e70079   2025.10（ ISSN:87567938 ）

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    Authorship：Corresponding author   Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    In microbial production, regulating endogenous and exogenous synthetic pathways is essential. Techniques to induce intracellular enzyme condensation have attracted attention as a means to increase apparent enzyme activity with minimal transcriptional and translational burden on the cell. Artificial enzyme condensation can be induced by tagging enzymes with specific proteins or peptides. In our previous study, we identified novel peptide tags derived from condensate-forming Saccharomyces cerevisiae glycolytic enzymes and their potential use in controlling intracellular metabolism by inducing artificial condensates in S. cerevisiae cells. Herein, we evaluated the condensate formation of two enzymes using peptide tags and the branched violacein biosynthetic pathway from Pseudoalteromonas luteoviolacea in S. cerevisiae, and tested the effects of modulating biosynthesis to increase the production of deoxyviolacein, a byproduct with antibacterial and anticancer activities. We used several protein and peptide tags with a simplified expression system, and all the tags successfully induced artificial condensate formation in the cell. Additionally, introducing a short peptide tag successfully increased deoxyviolacein production by approximately twofold, displaying a higher efficacy compared to FUSN, a previously reported N-terminal 213 amino acid region with an intrinsically disordered property. These results demonstrate the potential use of peptide tags to enhance bioproduction through the regulation of endogenous and exogenous synthetic pathways. The methods contribute to the development of novel strategies for microorganisms to be used for bioproduction through the controlled condensation of metabolic enzymes in cells.

    DOI： 10.1002/btpr.70079

    PubMed

  • Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR. Reviewed

    Tomoto Ura, Keita Saito, Ryoma Kobayashi, Natsuko Miura, Yoichi Takakusagi

    Journal of visualized experiments : JoVE   2025-July ( 221 )   2025.07（ ISSN:1940087X ）

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    Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    The protocol presents a method to integrate an in-house-built bioreactor system with hyperpolarized 13C nuclear magnetic resonance (NMR) spectroscopy to measure pyruvate metabolism in live cells in real time. The protocol outlines the construction of a bioreactor system that allows precise timing of hyperpolarized substrate injection and NMR signal acquisition while maintaining a stable cell culture environment. The method reliably detects the metabolic conversion of pyruvate to lactate within a 60 s measurement window while maintaining a sufficient signal-to-noise ratio throughout the process. The in situ mixing capability of the system accelerates NMR signal detection and reduces T1 relaxation losses. The protocol also includes detailed instructions for the preparation of alginate gels to encapsulate cells and place them within the bioreactor. The proposed method exhibits improved reproducibility and kinetic accuracy compared to conventional methods, indicating potential applications in cancer metabolism studies and drug screening. Further refinements of the system, such as the adoption of alternative gel matrices, may improve its versatility in various biological studies.

    DOI： 10.3791/68539

    PubMed

  • Compartmentation of multiple metabolic enzymes and their preparation in vitro and in cellulo. Invited Reviewed

    Sayoko Ito-Harashima, Natsuko Miura

    Biochimica et biophysica acta. General subjects   1869 ( 6 )   130787 - 130787   2025.03（ ISSN:03044165 ）

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    Authorship：Last author,　Corresponding author   Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    Compartmentalization of multiple enzymes in cellulo and in vitro is a means of controlling the cascade reaction of metabolic enzymes. The compartmentation of enzymes through liquid-liquid phase separation may facilitate the reversible control of biocatalytic cascade reactions, thereby reducing the transcriptional and translational burden. This has attracted attention as a potential application in bioproduction. Recent research has demonstrated the existence and regulatory mechanisms of various enzyme compartments within cells. Mounting evidence suggests that enzyme compartmentation allows in vitro and in vivo regulation of cellular metabolism. However, the comprehensive regulatory mechanisms of enzyme condensates in cells and ideal organization of cellular systems remain unknown. This review provides an overview of the recent progress in multiple enzyme compartmentation in cells and summarizes strategies to reconstruct multiple enzyme assemblies in vitro and in cellulo. By examining parallel examples, we have evaluated the consensus and future perspectives of enzyme condensation.

    DOI： 10.1016/j.bbagen.2025.130787

    PubMed

  • Deciphering mechanisms of UV filter (benzophenone-3)- and high temperature-induced adverse effects in the coral Acropora tenuis, using ecotoxicogenomics Reviewed

    Sakiko Nishioka, Kaede Miyata, Yasuaki Inoue, Kako Aoyama, Yuki Yoshioka, Natsuko Miura, Masayuki Yamane, Hiroshi Honda, Toshiyuki Takagi

    Science of The Total Environment   954 ( 1 )   176018 - 176018   2024.09（ ISSN:0048-9697 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.scitotenv.2024.176018

    PubMed

  • Real-Time Monitoring of Hydrolysis Reactions of Pyrophosphates with Dissolution Dynamic Nuclear Polarization. Reviewed

    Jun Fukazawa, Yuuki Mochizuki, Sakyo Kanai, Natsuko Miura, Makoto Negoro, Akinori Kagawa

    The journal of physical chemistry letters   15 ( 28 )   7288 - 7294   2024.07

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Dissolution dynamic nuclear polarization (d-DNP) has enabled applications such as the real-time monitoring of chemical reactions. Such applications are mainly for 13C and 15N spins with long spin-lattice relaxation times in the molecules of interest. However, the only applications for phosphorus using d-DNP are pH imaging and nucleation during crystallization due to the short relaxation times. Here we show that it is possible to observe enzyme reactions using d-DNP with phosphorus. Hyperpolarized 31P spins in pyrophosphate were obtained using bullet-DNP, which requires less dilution of highly polarized solid samples. Real-time monitoring of the hydrolysis reaction of pyrophosphate by inorganic pyrophosphatase from baker's yeast at physiological pH and was successfully achieved and the reaction rate was determined. This is an important reaction for a wide range of applications related to medicine, agriculture, and quantum life science.

    DOI： 10.1021/acs.jpclett.4c01456

    PubMed

  • Characterization of carotenoid-producing Muricauda sp. strains isolated from the reef-building coral Galaxea fascicularis and subtropical seawater

    Funa Endo, Keisuke Motone, Kenji Kai, Tomoya Kitakaze, Yumi Nishikawa, Yuna Nishimura, Ruriko Kitamura, Toshiyuki Takagi, Michihiro Ito, Natsuko Miura, Michihiko Kataoka

    bioRxiv   2023.12

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    Authorship：Corresponding author   Kind of work：Joint Work  

    ABSTRACT

    Bacterial symbionts in corals and coral-associated zooxanthellae are attracting increasing attention as potential probiotics. Some members of the family Flavobacteriaceae are zooxanthellae-associated bacteria, which are known to protect zooxanthellae from stresses such as heat and light by producing carotenoids that reduce reactive oxygen species production. This study characterized two Flavobacteriaceae bacteria,Muricaudasp. strains ORYM1 (NBRC115792) and ORYM2 (NBRC115793), isolated from the reef-building coralGalaxea fascicularisand its surrounding seawater in Okinawa, Japan, respectively. TheMuricaudasp. strain ORYM2 was isolated from subtropical seawater, and carotenoid production was assessed using ORYM2 as well as ORYM1, which was previously isolated fromG. fascicularis. De novogenome sequencing revealed that both strains contain complete sets of zeaxanthin biosynthesis genes, similar to those found in otherMuricaudaspp. Thin-layer chromatography and high-performance liquid chromatography analyses demonstrated that ORYM1 and ORYM2 produce several carotenoids. The bacterial strains and carotenoids identified in this study provide insights into the biological roles of zooxanthellae-associated bacteria in protecting zooxanthellae and reef-building corals from environmental stresses.

    Statements and declarations

    Competing interests: The authors declare no competing interests.

    DOI： 10.1101/2023.12.12.571358

  • Large-scale preparation of yeast strains expressing condensates derived from a glycolytic enzyme via controlled dissolved oxygen levels under hypoxia. Reviewed

    Yuki Murata, Reina Hirayama, Natsuko Miura, Michihiko Kataoka

    Letters in applied microbiology   76 ( 8 )   2023.08（ ISSN:0266-8254 ）

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    Authorship：Corresponding author   Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    Under hypoxia, Saccharomyces cerevisiae forms cytoplasmic condensates composed of proteins, including glycolytic enzymes, that are thought to regulate cellular metabolism. However, the hypoxic conditions required for the condensate formation remain unclear. In this study, we developed a 300-mL scale culture method to produce condensate-forming cells by precisely controlling the dissolved oxygen (DO) level in the media. Using enolase as a model, a foci formation rate of more than 50% was achieved at ∼0.1% DO, and the results showed that the DO level affected the foci formation rate. The foci formation rates of the previously reported foci-deficient strains and strains with single amino acid substitutions in the endogenous enolase were examined, and the effect of these amino acid substitutions on glucose consumption and ethanol and glycerol production under hypoxia were evaluated. The results of this study contribute to the investigation of the mechanisms that regulate biomacromolecular condensates under hypoxia.

    DOI： 10.1093/lambio/ovad095

    PubMed

  • Foci-forming regions of pyruvate kinase and enolase at the molecular surface incorporate proteins into yeast cytoplasmic metabolic enzymes transiently assembling (META) bodies. Reviewed

    Ryotaro Utsumi, Yuki Murata, Sayoko Ito-Harashima, Misaki Akai, Natsuko Miura, Kouichi Kuroda, Mitsuyoshi Ueda, Michihiko Kataoka

    PLOS ONE   18 ( 4 )   e0283002   2023.04

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    Authorship：Corresponding author   Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Spatial reorganization of metabolic enzymes to form the "metabolic enzymes transiently assembling (META) body" is increasingly recognized as a mechanism contributing to regulation of cellular metabolism in response to environmental changes. A number of META body-forming enzymes, including enolase (Eno2p) and phosphofructokinase, have been shown to contain condensate-forming regions. However, whether all META body-forming enzymes have condensate-forming regions or whether enzymes have multiple condensate-forming regions remains unknown. The condensate-forming regions of META body-forming enzymes have potential utility in the creation of artificial intracellular enzyme assemblies. In the present study, the whole sequence of yeast pyruvate kinase (Cdc19p) was searched for condensate-forming regions. Four peptide fragments comprising 27-42 amino acids were found to form condensates. Together with the fragment previously identified from Eno2p, these peptide regions were collectively termed "META body-forming sequences (METAfos)." METAfos-tagged yeast alcohol dehydrogenase (Adh1p) was found to co-localize with META bodies formed by endogenous Cdc19p under hypoxic conditions. The effect of Adh1p co-localization with META bodies on cell metabolism was further evaluated. Expression of Adh1p fused with a METAfos-tag increased production of ethanol compared to acetic acid, indicating that spatial reorganization of metabolic enzymes affects cell metabolism. These results contribute to understanding of the mechanisms and biological roles of META body formation.

    DOI： 10.1371/journal.pone.0283002

    PubMed

  • Mutualistic Interactions between Dinoflagellates and Pigmented Bacteria Mitigate Environmental Stress. Reviewed

    Toshiyuki Takagi, Kako Aoyama, Keisuke Motone, Shunsuke Aburaya, Hideyuki Yamashiro, Natsuko Miura, Koji Inoue

    Microbiology spectrum   11 ( 1 )   e0246422   2023.01

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Scleractinian corals form symbiotic relationships with a variety of microorganisms, including endosymbiotic dinoflagellates of the family Symbiodiniaceae, and with bacteria, which are collectively termed coral holobionts. Interactions between hosts and their symbionts are critical to the physiological status of corals. Coral-microorganism interactions have been studied extensively, but dinoflagellate-bacterial interactions remain largely unexplored. Here, we developed a microbiome manipulation method employing KAS-antibiotic treatment (kanamycin, ampicillin, and streptomycin) to favor pigmented bacteria residing on cultured Cladocopium and Durusdinium, major endosymbionts of corals, and isolated several carotenoid-producing bacteria from cell surfaces of the microalgae. Following KAS-antibiotic treatment of Cladocopium sp. strain NIES-4077, pigmented bacteria increased 8-fold based on colony-forming assays from the parental strain, and 100% of bacterial sequences retrieved through 16S rRNA amplicon sequencing were affiliated with the genus Maribacter. Microbiome manipulation enabled host microalgae to maintain higher maximum quantum yield of photosystem II (variable fluorescence divided by maximum fluorescence [Fv/Fm]) under light-stress conditions, compared to the parental strain. Furthermore, by combining culture-dependent and -independent techniques, we demonstrated that species of the family Symbiodiniaceae and pigmented bacteria form strong interactions. Dinoflagellates protected bacteria from antibiotics, while pigmented bacteria protected microalgal cells from light stress via carotenoid production. Here, we describe for the first time a symbiotic relationship in which dinoflagellates and bacteria mutually reduce environmental stress. Investigations of microalgal-bacterial interactions further document bacterial contributions to coral holobionts and may facilitate development of novel techniques for microbiome-mediated coral reef conservation. IMPORTANCE Coral reefs cover less than 0.1% of the ocean floor, but about 25% of all marine species depend on coral reefs at some point in their life cycles. However, rising ocean temperatures associated with global climate change are a serious threat to coral reefs, causing dysfunction of the photosynthetic apparatus of endosymbiotic microalgae of corals, and overproducing reactive oxygen species harmful to corals. We manipulated the microbiome using an antibiotic treatment to favor pigmented bacteria, enabling their symbiotic microalgal partners to maintain higher photosynthetic function under insolation stress. Furthermore, we investigated mechanisms underlying microalgal-bacterial interactions, describing for the first time a symbiotic relationship in which the two symbionts mutually reduce environmental stress. Our findings extend current insights about microalgal-bacterial interactions, enabling better understanding of bacterial contributions to coral holobionts under stressful conditions and offering hope of reducing the adverse impacts of global warming on coral reefs.

    DOI： 10.1128/spectrum.02464-22

    PubMed

  • Condensate formation by metabolic enzymes in Saccharomyces cerevisiae. Invited Reviewed OA

    Natsuko Miura

    Microorganisms   10 ( 2 )   232   2022.01

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    Authorship：Lead author,　Last author,　Corresponding author   Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Repository URL： http://hdl.handle.net/10466/00017827

  • Peptide barcoding for one-pot evaluation of sequence-function relationships of nanobodies. Reviewed

    Yusei Matsuzaki, Wataru Aoki, Takumi Miyazaki, Shunsuke Aburaya, Yuta Ohtani, Kaho Kajiwara, Naoki Koike, Hiroyoshi Minakuchi, Natsuko Miura, Tetsuya Kadonosono, Mitsuyoshi Ueda

    Scientific Reports 雑誌   11 ( 1 )   21516 - 21516   2021.11

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    Kind of work：Joint Work  

  • Simple Esterification of [1-13C]-Alpha-Ketoglutarate Enhances Membrane Permeability and Allows for Noninvasive Tracing of Glutamate and Glutamine Production. Reviewed

    Jenna E AbuSalim, Kazutoshi Yamamoto, Natsuko Miura, Burchelle Blackman, Jeffrey R Brender, Chandrasekhar Mushti, Tomohiro Seki, Kevin A Camphausen, Rolf E Swenson, Murali C Krishna, Aparna H Kesarwala

    ACS Chemical Biology 雑誌   16 ( 11 )   2144 - 2150   2021.09

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    Kind of work：Joint Work  

  • Specific Detection of Coral-Associated Ruegeria, a Potential Probiotic Bacterium, in Corals and Subtropical Seawater. Reviewed

    Ruriko Kitamura, Natsuko Miura, Michihiro Ito, Toshiyuki Takagi, Hideyuki Yamashiro, Yumi Nishikawa, Yuna Nishimura, Keita Kobayashi, Michihiko Kataoka

    Marine Biotechnology 雑誌   23   2021.07

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    Kind of work：Joint Work  

  • Synthesis of [1-13 C-5-12 C]-alpha-ketoglutarate enables noninvasive detection of 2-hydroxyglutarate. Reviewed

    Natsuko Miura, Chandrasekhar Mushti, Deepak Sail, Jenna E AbuSalim, Kazutoshi Yamamoto, Jeffrey R Brender, Tomohiro Seki, Deyaa I AbuSalim, Shingo Matsumoto, Kevin A Camphausen, Murali C Krishna, Rolf E Swenson, Aparna H Kesarwala

    NMR in biomedicine 雑誌   34 ( 11 )   2021.07

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    Kind of work：Joint Work  

  • Small-scale hypoxic cultures for monitoring the spatial reorganization of glycolytic enzymes in Saccharomyces cerevisiae. Reviewed

    Yuki Yoshimura, Reina Hirayama, Natsuko Miura, Ryotaro Utsumi, Kouichi Kuroda, Mitsuyoshi Ueda, Michihiko Kataoka

    Cell biology international 雑誌   45 ( 8 )   1776 - 1783   2021.04

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    Kind of work：Joint Work  

  • Design of novel primer sets for easy detection of Ruegeria species from seawater. Reviewed

    Kitamura R, Miura N*, Okada K, Motone K, Takagi T, Ueda M, Kataoka M.

    Bioscience, Biotechnology, and Biochemistry. 雑誌   84   854 - 864   2020

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    Kind of work：Joint Work  

  • A zeaxanthin-producing bacterium isolated from the algal phycosphere protects coral endosymbionts from environmental stress. Reviewed

    Motone K, Takagi T, Aburaya S, Miura N, Aoki W, Ueda M.

    mBio 雑誌   11   e01019 - 19   2020

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    Kind of work：Joint Work  

  • 選択的反応モニタリング質量分析のための1mの長さの一体型カラムの評価(Evaluation of meter-long monolithic columns for selected reaction monitoring mass spectrometry) Reviewed International coauthorship

    Ohtani Yuta, Aburaya Shunsuke, Minakuchi Hiroyoshi, Miura Natsuko, Aoki Wataru, Ueda Mitsuyoshi

    Journal of Bioscience and Bioengineering   128 ( 3 )   379 - 383   2019.09（ ISSN:1389-1723 ）

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    Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    内径が小さい1mの長さの一体型カラムを開発し、プロテオミクス的検出感度改善のための選択的反応モニタリング(SRM)に基づくプロテオミクスに使用した。ウシ血清アルブミントリプシン分解物を至適なSRM法で分析し、それぞれの一体型カラムでの分離効率と検出感度を評価した。その結果、ピークキャパシティーは約1.8倍、ピーク面積は約2.3倍に上昇した。小さな内径のカラムなので流速は低下したが、ピーク面積の再現性は維持された。以上より、内径の小さい1mの長さの一体型カラムは、SRMによるプロテオミクスに役立ちうると考えられた。

  • Domain swapping of complementarity-determining region in nanobodies produced by Pichia pastoris. Reviewed

    Miura N, Miyamoto K, Ohtani Y, Yaginuma K, Aburaya S, Kitagawa Y, Aoki W, Ueda M

    AMB Express 雑誌   9 ( 1 )   2019.07

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    Kind of work：Joint Work  

  • High-throughput identification of peptide agonists against GPCRs by co-culture of mammalian reporter cells and peptide-secreting yeast cells using droplet microfluidics. Reviewed

    Yaginuma K, Aoki W, Miura N, Ohtani Y, Aburaya S, Kogawa M, Nishikawa Y, Hosokawa M, Takeyama H, Ueda M

    Scientific reports 雑誌   9 ( 1 )   2019.07

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    Kind of work：Joint Work  

  • Peptide barcoding for establishment of new types of genotype-phenotype linkages. Reviewed

    Miyamoto K, Aoki W, Ohtani Y, Miura N, Aburaya S, Matsuzaki Y, Kajiwara K, Kitagawa Y, Ueda M.

    PLoS One 雑誌   14 ( 4 )   2019.04

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    Kind of work：Joint Work  

  • Evaluation of meter-long monolithic columns for selected reaction monitoring mass spectrometry. Reviewed

    Ohtani Y, Aburaya S, Minakuchi H, Miura N, Aoki W, Ueda M

    Journal of bioscience and bioengineering 雑誌   128 ( 3 )   379 - 383   2019.04

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    Kind of work：Joint Work  

  • Ruegeria sp. Strains Isolated from the Reef-Building Coral Galaxea fascicularis Inhibit Growth of the Temperature-Dependent Pathogen Vibrio coralliilyticus Reviewed

    Miura N, Motone K, Takagi T, Aburaya S, Watanabe S, Aoki W, Ueda M

    Marine Biotechnology 雑誌 Springer Nature   2018.09

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    Kind of work：Joint Work  

  • Protection of Coral Larvae from Thermally Induced Oxidative Stress by Redox Nanoparticles. Reviewed

    Motone K, Takagi T, Aburaya S, Aoki W, Miura N, Minakuchi H, Takeyama H, Nagasaki Y, Shinzato C, Ueda M.

    Marine Biotechnology 雑誌   20   542 - 548   2018.08

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    Kind of work：Joint Work  

  • Evaluation of Unconventional Protein Secretion by Saccharomyces cerevisiae and other Fungi Reviewed

    Miura N, Ueda M

    cells 雑誌 MDPI AG, Basel, Switzerland   7 ( 9 )   2018.08

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    Kind of work：Joint Work  

  • Three-Dimensional Alginate Hydrogels for Radiobiological and Metabolic Studies of Cancer Cells Reviewed

    Read GH, Miura N, Carter JL, Kines KT, Devasahayam N, Cheng JY, Camphausen KA, Krishna MC, Kesarwala AH.

    Colloids and Surfaces B: Biointerfaces 雑誌   171   197 - 204   2018.06

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    Kind of work：Joint Work  

  • Enzyme Evolution.

    Miura N

    Yeast Cell Surface Engineering – Biological Mechanism and Its Practical Applications. 著書 Springer Nature   2018

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    Kind of work：Single Work  

  • Reconstruction of Thermotolerant Yeast by One-Point Mutation Identified through Whole-Genome Analyses of Adaptively-Evolved Strains. Reviewed

    Satomura A, Miura N, Kuroda K, Ueda M.

    Scientific Reports 雑誌   6   2016.03

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    Kind of work：Joint Work  

  • Enzyme Evolution by Yeast Cell Surface Engineering.

    Miura N, Kuroda K, Ueda M.

    Methods in Molecular Biology 著書   1319   2015

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    Kind of work：Joint Work  

  • Evaluation of Unconventional Protein Secretion in Saccharomyces cerevisiae.

    Miura N, Ueda M.

    Methods in Molecular Biology 著書   1270   2015

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    Kind of work：Joint Work  

  • Single-cell Heterogeneity in Suppression of PC12 Differentiation by Direct Microinjection of a Differentiation Inhibitor, U0126. Reviewed

    Chung J, Miura N, Ito A, Sawada M, Nishikawa S, Kuroda K, Ueda M.

    Cell Biology International 雑誌   38 ( 10 )   2014

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    Kind of work：Joint Work  

  • Application of Cell Surface Engineered Yeast for the Development of Drugs Against Influenza Virus Infection.

    Yamada J, Miura N, Shigemori T, Kuroda K, Ueda M.

    Influenza and RNA Viruses: Emergence, Classification and Management. 著書 Nova Science Publishers   2014

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    Kind of work：Joint Work  

  • Spatial Reorganization of Saccharomyces cerevisiae Enolase To Alter Carbon Metabolism under Hypoxia. Reviewed

    Miura N, Shinohara M, Tatsukami Y, Sato Y, Morisaka H, Kuroda K, Ueda M.

    Eukaryotic Cell 雑誌   12 ( 8 )   2013

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    Kind of work：Joint Work  

  • Mutant Firefly Luciferases with Improved Specific Activity and dATP Discrimination Constructed by Yeast Cell Surface Engineering. Reviewed

    Fushimi T, Miura N, Shintani H, Tsunoda H, Kuroda K, Ueda M.

    Applied Microbiology and Biotechnology 雑誌   97 ( 9 )   2013

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    Kind of work：Joint Work  

  • Acquisition of Thermotolerant Yeast Saccharomyces cerevisiae by Breeding via Stepwise Adaptation. Reviewed

    Satomura A, Katsuyama Y, Miura N, Kuroda K, Tomio A, Bamba T, Fukusaki E, Ueda M.

    Biotechnology Progress 雑誌   29   2013

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    Kind of work：Joint Work  

  • Construction of a Convenient System for Easily Screening Inhibitors of Mutated Influenza Virus Neuraminidases. Reviewed

    Shigemori T, Nagayama M, Yamada J, Miura N, Yongkiettrakul S, Kuroda K, Katsuragi T, Ueda M.

    FEBS Open Bio 雑誌   3   2013

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    Kind of work：Joint Work  

  • Tracing Putative Trafficking of the Glycolytic Enzyme Enolase via SNARE-Driven Unconventional Secretion. Reviewed

    Miura N, Kirino A, Endo S, Morisaka H, Kuroda K, Takagi M, Ueda M.

    Eukaryotic Cell 雑誌   11 ( 8 )   2012

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    Kind of work：Joint Work  

  • Design of a Novel Antimicrobial Peptide Activated by Virulent Proteases. Reviewed

    Aoki W, Kitahara N, Miura N, Morisaka H, Kuroda K, Ueda M.

    Chemical Biology & Drug Design 雑誌   80 ( 5 )   2012

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    Kind of work：Joint Work  

  • Candida albicans Possesses Sap7 as a Pepstatin A-Insensitive Secreted Aspartic Protease. Reviewed

    Aoki W, Kitahara N, Miura N, Morisaka H, Yamamoto Y, Kuroda K, Ueda M.

    PLoS ONE 雑誌   7   2012

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    Kind of work：Joint Work  

  • Profiling of Adhesive Properties of the Agglutinin-like Sequence (ALS) Protein Family, a Virulent Attribute of Candida albicans. Reviewed

    Aoki W, Kitahara N, Miura N, Morisaka H, Kuroda K, Ueda M.

    FEMS Immunology and Medical Microbiology 雑誌   65   2012

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    Kind of work：Joint Work  

  • Comprehensive Characterization of Secreted Aspartic Proteases Encoded by a Virulence Gene Family in Candida albicans. Reviewed

    Aoki W, Kitahara N, Miura N, Morisaka H, Yamamoto Y, Kuroda K, Ueda M.

    Journal of Biochemistry 雑誌   150 ( 4 )   2011

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    Kind of work：Joint Work  

  • Cell-Surface Modification of Non-GMO without Chemical Treatment by Novel GMO-Coupled and-Separated Cocultivation Method. Reviewed

    Miura N, Aoki W, Tokumoto N, Kuroda K, Ueda M.

    Applied Microbiology and Biotechnology 雑誌   82 ( 2 )   2009

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    Kind of work：Joint Work  

  • Surface Coat Proteins of the Pine Wood Nematode, Bursaphelenchus xylophilus: Profiles of Stage and Isolate-Specific Characters. Reviewed

    Shinya R, Takeuchi Y, Miura N, Kuroda K, Ueda M, Futai K.

    Nematology 雑誌   11   2009

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    Kind of work：Joint Work  

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Books and Other Publications

  • マリンバイオテクノロジー

    三浦夏子 (担当:分担執筆, 範囲:大西康夫・小川順 編, 応用微生物学 第4版 pp. 338-344)

    文永堂出版  2025.02  （ ISBN:9784830041464 ）

  • 低酸素条件下で形成する代謝酵素集合体の観察

    三浦夏子（ Role： Contributor ,  加藤昌人・白木賢太郎・中川真一編, フロントランナー直伝 相分離解析プロトコール (pp.135-145)）

    羊土社  2022.07 

  • 酵素工学への展開

    三浦夏子, 片岡道彦（ Role： Joint author）

    シーエムシー出版  2020.04 

  • ムーンライティング酵素の展開

    三浦夏子, 片岡道彦（ Role： Joint author）

    シーエムシー出版  2019.05  （ ISBN:978-4-7813-1411-2 ）

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    Responsible for pages：247-258  

  • Enzyme Evolution

    Miura N（ Role： Sole author）

    Springer Nature  2019  （ ISBN:978-981-13-5867-8 ）

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    Responsible for pages：175-185  

  • 非侵襲的代謝診断の臨床応用（実用化）に向けたビッグデータ活用への期待

    三浦夏子（ Role： Sole author）

    シーエムシー出版  2018.02  （ ISSN: 978-4-7813-1315-3 ）

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    Responsible for pages：66-74  

  • 変異タンパク質およびタンパク質ドメインを用いたshort RNAの回収、分離技術

    三浦夏子, 林絵理, 植田充美（ Role： Joint author）

    シーエムシー出版  2010 

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MISC

  • 細胞内解糖系酵素の集合原理とその応用に関する研究 Invited International journal

    三浦 夏子

    バイオサイエンスとインダストリー   83 ( 5 )   416 - 417   2025.09

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    Authorship：Lead author,　Last author,　Corresponding author   Publishing type：Article, review, commentary, editorial, etc. (other)   Kind of work：Single Work   International / domestic magazine：Domestic journal  

  • 出芽酵母で形成される解糖系酵素群の集合体とその制御 Invited Reviewed

    三浦夏子

    日本農芸化学会 会誌「化学と生物」   62 ( 4 )   181 - 186   2024.04

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    Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

  • 【若手研究者が拓くこれからの生物工学(前編)】生体内の代謝を知る・みる・使う Invited

    三浦 夏子

    生物工学会誌   100 ( 5 )   240 - 245   2022.05（ ISSN:0919-3758 ）

  • 解糖系酵素の局在制御とリンクした代謝制御ー集まると仕事が早く進む？ Invited

    三浦夏子

    日本農芸化学会 会誌「化学と生物」   58 ( 1 )   10 - 12   2020.01

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    Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

  • 低酸素条件下で形成される代謝酵素集合体G-body の発見 Invited International journal

    三浦夏子

    酵素工学研究会 酵素工学ニュース   ( 80 )   5 - 10   2018.10

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  • Coral Symbionts Invited International journal

    Natsuko Miura

    Journal of Bioscience and Bioengineering   96 ( 8 )   2018.08

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  • 金属イオン吸着・回収に向けた細胞表層デザインと吸着分子の創製

    黒田浩一, 三浦夏子

    日本生物工学会誌   2008

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  • バイオマスデザインに向けた植物育種の新発想

    黒田浩一, 三浦夏子, 植田充美

    BIOINDUSTRY   2008

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Presentations

  • 低酸素により誘導される解糖系酵素群集合体の制御基盤の開発 Invited Domestic conference

    三浦 夏子

    日本薬学会第146年会（大会）一般シンポジウムS34「学術変革領域研究（B)「特化メタボロン」：タンパク質の自己集合研究が拓く新たな薬学」  2026.03 

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    Presentation type：Symposium, workshop panel (nominated)  

  • Hypoxia-induced enzyme condensation and its function Invited Domestic conference

    Natsuko Miura

    The 99th Annual Meeting of the Japanese Pharmacological Society  2026.03 

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    Presentation type：Symposium, workshop panel (nominated)  

  • Analysis of G-body formation characteristics in Saccharomyces cerevisiae YPH499 Domestic conference

    Mana YAMAGUCHI, Megumi UYAMA, Natsuko MIURA, Michihiko KATAOKA

    The 2026 Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry  2026.03 

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    Presentation type：Oral presentation (general)  

  • Investigation of biodegradable plastic-degrading bacteria in Osaka Bay Invited Domestic conference

    Natsuko Miura

    The 2026 Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry  2026.03 

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    Presentation type：Symposium, workshop panel (nominated)  

  • Evaluation of the efficiency improvement of (R)-3-quinuclidinol production using a condensate-forming domain Domestic conference

    Miyu YAMASHITA, Seiji KOBAYASHI, Yuki MUROSHIGE, Sayoko HARASHIMA, Natsuko Miura, Michihiko KATAOKA

    The 2026 Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry  2026.03 

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    Presentation type：Oral presentation (general)  

  • Protein Semi-quantification Through Fluorescence-Based Assessment of Saccharomyces cerevisiae under hypoxia Domestic conference

    Shota NAKASHIMA, Misaki AKAI, Honoka MORIKAWA, Natsuko MIURA, Michihiko KATAOKA

    The 2026 Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry  2026.03 

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    Presentation type：Oral presentation (general)  

  • Identification of trigger factors regulating G-body formation in hypoxic culture Domestic conference

    Yui KAWASHIMA, Misaki AKAI, Honoka MORIKAWA, Natsuko MIURA, Michihiko KATAOKA

    The 2026 Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry  2026.03 

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    Presentation type：Oral presentation (general)  

  • Intracellular condensate formation of glycolytic enzymes Invited International conference

    Natsuko Miura

    NIG-JOINT 2025 International Symposium on Molecular Biology and Microbiology: Frontiers in microbial research providing a fundamental insight into conserved and diverse nature of cell duplication system  2026.03 

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    Presentation type：Symposium, workshop panel (nominated)  

  • 低酸素に応答して局在変化する解糖系酵素 ーその機序の理解と応用に向けて Invited Domestic conference

    三浦 夏子

    日本農芸化学会中四国支部第73回講演会（例会）特別シンポジウム「細胞内の分子を覗き見る農芸化学」  2026.01 

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    Presentation type：Symposium, workshop panel (nominated)  

  • Microbial engineering for sustainable marine biotechnology Invited International conference

    Natsuko Miura

    Pacifichem 2025 Symposium CES009A Biotechnological Approaches for Sustainable Bioeconomy: Integrating Microbial Engineering with Green and Blue Carbon Strategies  2025.12 

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    Presentation type：Symposium, workshop panel (nominated)  

  • 低温超偏極13C-NMR/MRIを⽤いた⽣細胞のリアルタイム代謝測定 Invited Domestic conference

    三浦 夏子

    進化トロニクス勉強会 online  2025.12 

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    Presentation type：Public lecture, seminar, tutorial, course, or other speech  

  • Discovery and analysis of hypoxia-responsive glycolytic enzyme assemblies in Saccharomyces cerevisiae Invited Domestic conference

    Natsuko Miura

    Ohsumi Life Science Research Society Young Scientist Award Lecture  2025.12 

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    Presentation type：Oral presentation (invited, special)  

  • Condensates of glycolytic enzymes formed under hypoxic conditions: dynamics, functions and their regulation Invited Domestic conference

    Natsuko Miura

    The 48th Annual Meeting of the Molecular Biology Society Japan  2025.12 

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    Presentation type：Symposium, workshop panel (nominated)  

  • Isolation and identification of marine microorganisms thatdegrade Poly[( R )-3-hydroxybutyrate-co-( R )-3-hydroxyhexanoate] from Osaka Bay Domestic conference

    Takumi Iida, Mana Ninomiya, Sung Yi Shiuan, Ami Kakinuma, Keita Kobayashi, Natsuko Miura, Michihiko Kataoka

    The 48th Annual Meeting of the Molecular Biology Society Japan  2025.12 

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    Presentation type：Poster presentation  

  • Basic research to elucidate the mechanism of formation of G-body, an assembly of glycolytic enzymes and other proteins, using a jar fermenter Domestic conference

    Honoka Morikawa, Natsuko Miura, Michihiko Kataoka

    The 48th Annual Meeting of the Molecular Biology Society Japan  2025.12 

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    Presentation type：Poster presentation  

  • Analysis of enolase condensates formed in vitro Domestic conference

    Koharu Alicia Senda, Shiho Minamikata, Yoshiaki Sakurai, Natsuko Miura, Michihiko Kataoka

    The 48th Annual Meeting of the Molecular Biology Society Japan  2025.12 

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    Presentation type：Poster presentation  

  • Effects of dissolved oxygen and temperature on enolase foci formation in Saccharomyces cerevisiae Domestic conference

    Megumi Uyama, Natsuko Miura, Michihiko Kataoka

    The 48th Annual Meeting of the Molecular Biology Society Japan  2025.12 

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    Presentation type：Poster presentation  

  • Interaction between coral symbiotic bacteria using isolated co-culture vessels and analysis of growth inhibitor Domestic conference

    Shuka Kamiyama, Rei Nakashima, Takumi Iida, Yumi Nishikawa, Shozo Fukaya, Natsuko Miura, Mitsuyoshi Ueda, Michihiko Kataoka

    The 48th Annual Meeting of the Molecular Biology Society Japan  2025.12 

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    Presentation type：Poster presentation  

  • Investigation of the G-body formation and regulation by Dorsomorphin in HepG2 cell line under hypoxia Domestic conference

    Hiroko Ogasawara, Michihiko Kataoka, Natsuko Miura

    The 48th Annual Meeting of the Molecular Biology Society Japan  2025.12 

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    Presentation type：Poster presentation  

  • Construction and optimization of a cofactor regeneration system using glucose dehydrogenase and horseradish peroxidase International conference

    Nanako Tanaka, Natsuko Miura, Michihiko Kataoka

    International Conference on Bottom-up Biotechnology for Understanding and Engineering Living Systems 2025  2025.11 

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    Presentation type：Poster presentation  

  • Enhancement of glucose dehydrogenase (GDH) activity in the presence of high concentration of diverse proteins International conference

    Sayoko Ito-Harashima, Ako Kagawa, Seiji Kobayashi, Yuki Muroshige, Tomoaki Matsuura, Natsuko Miura, Michihiko Kataoka

    International Conference on Bottom-up Biotechnology for Understanding and Engineering Living Systems 2025  2025.11 

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    Presentation type：Poster presentation  

  • Analysis of enolase condensates formed in vitro International conference

    Koharu Alicia Senda, Natsuko Miura, Michihiko Kataoka

    International Conference on Bottom-up Biotechnology for Understanding and Engineering Living Systems 2025  2025.11 

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    Presentation type：Poster presentation  

  • Discovery of assembly forming peptides functioning in cells and their designability Invited Domestic conference

    Natsuko Miura

    The 98th Annual Meeting of the Japanese Biochemical Society  2025.11 

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    Presentation type：Symposium, workshop panel (nominated)  

  • Construction of small-scale screening system to detect assembly forming sequences using deoxyviolacein biosynthetic pathway International conference

    Naoko Segawa, Rioko Ito, Sayoko Ito-Harashima, Natsuko Miura, Michihiko Kataoka

    International Conference on Bottom-up Biotechnology for Understanding and Engineering Living Systems 2025  2025.11 

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    Presentation type：Poster presentation  

  • 出芽酵母細胞内における解糖系酵素集合体の発見とその解析・利用に関する研究 Domestic conference

    三浦 夏子

    BioJapan 出展者プレゼンテーション  2025.10 

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    Presentation type：Public lecture, seminar, tutorial, course, or other speech  

  • Elucidation and Utilization of Metabolic Regulation Mechanisms through Changes in Metabolic Enzyme Localization Invited Domestic conference

    Natsuko Miura

    The 77th Annual Meeting of the Society of Biotechnology of Japan (SBJ)KSBB-BEST-SBJ joint symposium (Session 2) Shaping the Next Wave: Young Innovators in Engineering Biology  2025.09 

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    Presentation type：Public lecture, seminar, tutorial, course, or other speech  

  • Research on the Assembly Mechanism of Intracellular Glycolytic Enzymes and Its Applications Invited Domestic conference

    Natsuko Miura

    The 77th Annual Meeting of the Society of Biotechnology of Japan (SBJ)KSBB-BEST-SBJ joint symposium (Session 2) Shaping the Next Wave: Young Innovators in Engineering Biology  2025.09 

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    Presentation type：Oral presentation (invited, special)  

  • 分離共培養容器を用いた サンゴ病原菌Vibrio coralliilyticusおよび Ruegeria属細菌の発現変動遺伝子の探索 Domestic conference

    飯田拓海, 中島玲, 神山殊果, 西川有美, 深谷昭三, 三浦夏子, 植田充美, 片岡道彦

    2025年 生物工学若手研究者の集い（若手会）夏のセミナー ...  2025.07 

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    Presentation type：Poster presentation  

  • グルコース脱水素酵素とホースラディッシュペルオキシダーゼによる補酵素再生系の構築 Domestic conference

    田中菜奈子, 三浦夏子, 片岡道彦

    2025年 生物工学若手研究者の集い（若手会）夏のセミナー ...  2025.07 

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    Presentation type：Poster presentation  

  • In vitroにおけるエノラーゼ集合体の形成条件の解析 Domestic conference

    千田小春アリシア, 三浦夏子, 片岡道彦

    2025年 生物工学若手研究者の集い（若手会）夏のセミナー ...  2025.07 

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    Presentation type：Poster presentation  

  • Deoxyviolacein生合成経路を用いた集合体形成配列のスクリーニングに利用可能な小スケール実験系の確立 Domestic conference

    瀨川奈緒子, 伊藤里緒子, 原島小夜子, 三浦夏子, 片岡道彦

    2025年 生物工学若手研究者の集い（若手会）夏のセミナー ...  2025.07 

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    Presentation type：Poster presentation  

  • 代謝酵素集合体の形成による代謝制御機構とその展開 Invited Domestic conference

    三浦 夏子

    近畿化学協会バイオ部会第１回例会  2025.06 

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    Presentation type：Symposium, workshop panel (public)  

  • 医学への展開を見据えたDNP技術による生物の代謝解析 Invited Domestic conference

    三浦 夏子

    第五回量子超偏極スタディグループ「量子超偏極の生命科学と知的基盤形成」, online  2025.06 

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    Presentation type：Public lecture, seminar, tutorial, course, or other speech  

  • Designer Enzyme Condensates for Tunable Biocatalysis International conference

    Natsuko Miura

    4th Swiss-German-Japanese Workshop on Biocatalysis and Bioprocess Development (JCHD2025), University of Greifswald, Germany  2025.06 

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    Presentation type：Oral presentation (general)  

  • 酵素集合体の形成による細胞の代謝制御 Invited Domestic conference

    三浦 夏子

    早稲⽥⼤学 先進理⼯学部応⽤化学科 講演会 「応⽤⽣物化学の最前線」  2025.06 

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    Presentation type：Public lecture, seminar, tutorial, course, or other speech  

  • 共用機器の活用で広がる研究の可能性： 生命環境科学と工学の融合による新たな挑戦』 Invited Domestic conference

    三浦 夏子

    大阪公立大学研究推進機構研究基盤共用センターシンポジウム「研究基盤共用センターのこれから」  2025.06 

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    Presentation type：Symposium, workshop panel (nominated)  

  • 超偏極13C-NMRによるがん細胞の低酸素応答測定 Domestic conference

    小笠原弘子, 齋藤圭太, 小池歩, 志牟田宜子, 片岡道彦, 高草木洋一, 三浦夏子

    量子生命科学会 第７回大会  2025.05 

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    Presentation type：Poster presentation  

  • 分離共培養条件下におけるサンゴ病原菌Vibrio coralliilyticus およびRuegeria属細菌の発現変動遺伝子の探索 Domestic conference

    飯田拓海, 中島玲, 神山殊果, 西川有美, 深谷昭三, 三浦夏子, 植田充美, 片岡道彦

    第25回マリンバイオテクノロジー学会  2025.05 

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    Presentation type：Poster presentation  

  • サンゴ共生系の維持に関わる分子の産生機構 Invited

    三浦夏子

    自然共生に学ぶ生物工学研究部会 第1回オープンセミナー  2025.03 

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    Presentation type：Public lecture, seminar, tutorial, course, or other speech  

  • Metabolic enzyme 'droplets' formed in cells under hypoxic conditions: components and control of their dynamics Invited

    Natsuko Miura

    The 2025 Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry  2025.03 

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    Presentation type：Symposium, workshop panel (nominated)  

  • A machine learning-based quantitative analysis method by classification of cytoplasmic condensate-forming cells in Saccharomyces cerevisiae

    Ryuta Saito, Natsuko Miura, Yuki Yoshimura, Kohei Tanaka, Michihiko Kataoka

    The 2025 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry  2025.03 

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    Presentation type：Poster presentation  

  • Analysis of the response of Ruegeria spp. to the coral pathogen Vibrio coralliilyticus under separate co-culture condition

    Takumi Iida, Rei Nakashima, Shuka Kamiyama, Yumi Nishikawa, Shozo Fukaya, Natsuko Miura, Mitsuyoshi Ueda, Michihiko Kataoka

    The 2025 Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry  2025.03 

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    Presentation type：Poster presentation  

  • 出芽酵母細胞内における解糖系酵素集合体の発見とその解析・利用に関する研究 Invited

    三浦 夏子

    バイオインダストリー奨励賞受賞者特別企画講演 第2弾「自然の複雑さを紐解き細胞の可能性を引き出し種を超える～次世代バイオが築くイノベーションの夜明け～」  2025.02 

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    Presentation type：Public lecture, seminar, tutorial, course, or other speech  

  • In vitro evaluation of cell metabolism by hyperpolarized 13C-NMR and future biological applications Invited

    Natsuko Miura

    Quantum Innovation 2024 Symposium (Quantum Sensing Track) SE07 Quantum MRI  2024.10 

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    Presentation type：Symposium, workshop panel (nominated)  

  • Enhancing bioproduction through peptide-tag mediated enzyme condensation Invited

    The 76th Annual Meeting of the Society of Biotechnology of Japan (SBJ)KSBB-BEST-SBJ joint symposium (Session 2) Shaping the Next Wave: Young Innovators in Engineering Biology  2024.09 

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    Presentation type：Symposium, workshop panel (public)  

  • 出芽酵母で低酸素下で形成される解糖系酵素等の集合体G-bodyの単離・精製に向けた基礎研究

    森川 穂香, 赤井 美咲, 三浦 夏子, 片岡 道彦

    日本農芸化学会関西支部 第531回講演会（学生優秀発表賞）  2024.07 

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    Presentation type：Oral presentation (general)  

  • Quantitative Analytical Method Based on Machine Learning by Classification of Condensate Forming Cells by Glycolytic Enzymes in Saccharomyces cerevisiae

    Natsuko Miura, Ryuta Saito, Yuki Yoshimura, Kohei Tanaka, Michihiko Kataoka

    IUPAB2024  2024.06 

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    Presentation type：Poster presentation  

  • Formation and Regulation of Metabolic Enzyme Assemblies in Cells Invited

    Natsuko Miura

    he 18th Annual Meeting of Japanese Society for Chemical Biology  2024.05 

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    Presentation type：Symposium, workshop panel (nominated)  

  • Observation of time-dependent G-body formation rate in hypoxic culture

    Honoka Morikawa, Misaki Akai, Natsuko Miura, Michihiko Kataoka

    The 2024 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry  2024.03 

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    Presentation type：Oral presentation (general)  

  • Interaction between coral symbiotic bacteria using isolated co-culture vessels and analysis of physiologically active substances

    Shuka Kamiyama, Rei Nakashima, Yumi Nishikawa, Shozo Fukaya, Natsuko Miura, Mitsuyoshi Ueda, Michihiko Kataoka

    The 2024 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry  2024.03 

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    Presentation type：Oral presentation (general)  

  • Verification of changes in enolase activity and mutant activity under hypoxic conditions

    Narumi Washio, Natsuko Miura, Michihiko Kataoka

    The 2024 Annual Meeting of the Japan Society for Bioscience, Biotechnology, and Agrochemistry  2024.03 

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    Presentation type：Oral presentation (general)  

  • 変異型RNaseを用いた酵素集合体"META body"形成に寄与するRNA分子の探索

    三浦 夏子

    日本応用酵素協会誌  2024.03  (公財)日本応用酵素協会

  • Moonlighting glycolytic enzymes in Saccharomyces cerevisiae Invited Domestic conference

    Natsuko Miura

    The 46th Annual Meeting of the Molecular Biology Society of Japan  2023.12 

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    Presentation type：Oral presentation (invited, special)  

  • Liquid-liquid phase separation of enzymes Invited Domestic conference

    Natsuko Miura

    The 46th Annual Meeting of the Molecular Biology Society of Japan  2023.12 

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    Presentation type：Oral presentation (invited, special)  

  • Liquid-liquid phase separation of glycolytic enzymes Invited Domestic conference

    Natsuko Miura

    The 61st Annual Meeting of the BSJ Symposium: The forefront of biophysical research of liquid-liquid phase separation  2023.11 

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    Presentation type：Symposium, workshop panel (public)  

  • Emerging Functions of Coral- and Zooxanthellae-Associated Bacteria Invited Domestic conference

    Natsuko Miura

    2023.09 

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    Presentation type：Oral presentation (invited, special)  

  • 海洋生分解性プラスチックを分解する酵素PhaZの異種発現系構築 および分解活性測定方法の検討について Domestic conference

    柿沼 亜望, 三浦 夏子, 片岡 道彦

    第75回日本生物工学会大会  2023.09 

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    Presentation type：Oral presentation (general)  

  • Flux regulation in the violacein biosynthesis pathway using enzyme assembly formation Domestic conference

    Rioko Ito, Natsuko Miura, Michihiko Kataoka

    The 75th Annual Meeting of the Society of Biotechnology of Japan  2023.09 

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    Presentation type：Oral presentation (general)  

  • 低酸素条件下における代謝酵素群による集合体形成の発見およびその制御機構の解析 Invited Domestic conference

    三浦夏子

    ⽇本農芸化学会関⻄⽀部 第509 回講演会 受賞記念講演  2023.07 

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    Presentation type：Oral presentation (invited, special)  

  • 代謝酵素集合体 G-body の形成を制御する低分子化合物の探索 Domestic conference

    水野有沙, 三浦夏子, 片岡道彦

    生物工学若手研究者の集い（若手会）夏のセミナー2023  2023.06 

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    Presentation type：Poster presentation  

  • 細胞における代謝酵素群の集合体形成に学ぶ Invited

    三浦 夏子

    分子研ExCELLS研究会「生命の分子システムの理解に向けて何を創れば良いか？」  2023.05 

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    Presentation type：Oral presentation (invited, special)  

  • 低酸素条件下における解糖系酵素の集合体形成の発見およびその制御機構の解析. Invited

    三浦夏子

    酵素工学研究会第８９回講演会 2022年度酵素工学奨励賞受賞講演.  2023.04 

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    Presentation type：Oral presentation (invited, special)  

  • METAbody形成タグを用いた酵素集合体の人為的な構築 Invited

    三浦夏子

    学術変革領域研究(A)分子サイバネティクス・超越分子システム合同シンポジウム2023  2023.03 

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    Presentation type：Oral presentation (invited, special)  

  • 低酸素条件下で細胞質に集合体を形成する解糖系酵素 Invited

    三浦 夏子

    第9回タタバイオ分子クラブ  2023.03 

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    Presentation type：Oral presentation (invited, special)  

  • 低酸素条件下における代謝酵素群による集合体形成の発見およびその制御機構の解析. Invited

    三浦夏子

    日本農芸化学会2023年度大会 農芸化学若手女性研究者賞受賞者講演  2023.03 

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    Presentation type：Oral presentation (invited, special)  

  • Exploring the principle of formation of the META body, a condensate formed by a group of central metabolic enzymes in the cytoplasm Invited

    Natsuko Miura

    The 45th Annual Meeting of the Molecular Biology Society of Japan  2022.11 

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    Presentation type：Symposium, workshop panel (nominated)  

  • 解糖系酵素が形成する細胞内集合体とその制御 Invited Domestic conference

    三浦夏子

    第74回日本生物工学会大会シンポジウム「S23 最先端の代謝研究が解き明かす解糖系の深淵—Otto Meyerhof ノーベル賞受賞100周年によせて—」  2022.10 

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    Presentation type：Symposium, workshop panel (nominated)  

  • 沖縄県のサンゴと海水から単離されたMuricauda属細菌 ORYM1, ORYM2株の機能探索

    遠藤芙奈, 西川有美, 高木俊幸, 三浦夏子, 片岡道彦

    ワークショップ「サンゴ礁生態系におけるレドックスエコバイオロジー」  2022.10 

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    Presentation type：Poster presentation  

  • 分離共培養容器を用いたサンゴ共在細菌間相互作用の実験的再構築

    中島玲, 西川有美, 三浦夏子, 植田充美, 片岡道彦

    ワークショップ「サンゴ礁生態系におけるレドックスエコバイオロジー」  2022.10 

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    Presentation type：Poster presentation  

  • 生命を駆動する中心代謝酵素の新たな挙動－酵素集合体形成による可逆的な細胞内代謝区画の形成 Invited

    三浦夏子

    第73回コロイド及び界面化学討論会  2022.09 

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    Presentation type：Symposium, workshop panel (nominated)  

  • 低酸素条件下での集合体形成による細胞の代謝調節 Invited

    三浦夏子

    2022年日本農芸化学会関西支部大会（第522回講演会）  2022.09 

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    Presentation type：Oral presentation (invited, special)  

  • 低酸素条件下で形成される代謝酵素集合体−細胞内の新たな代謝調節機構？ Invited

    三浦夏子

    日本環境変異原ゲノム学会変異機構研究会・第33回夏の学校  2022.09 

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    Presentation type：Oral presentation (invited, special)  

  • G-body形成に最適な酵母低酸素培養系の評価

    村田有紀, 吉村柚紀, 平山伶奈, 内海亮太郎, 伊藤里緒子, 三浦夏子, 片岡道彦

    日本農芸化学会関西支部 第521回講演会  2022.07 

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    Presentation type：Oral presentation (general)  

  • 沖縄県のサンゴと海水から単離されたMuricauda属細菌 ORYM1, ORYM2株の機能探索

    遠藤芙奈, 西川有美, 高木俊幸, 三浦夏子, 片岡 道彦

    第22回マリンバイオテクノロジー学会大会  2022.05 

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    Presentation type：Poster presentation  

  • Specific Detection of Coral‑Associated Ruegeria, a Potential Probiotic Bacterium, in Corals and Subtropical Seawater Invited

    北村瑠璃子, 三浦夏子, 伊藤通浩, 高木俊幸, 山城秀之, 西川有美, 西村優菜, 小林慶太, 片岡道彦

    第22回マリンバイオテクノロジー学会大会 論文賞受賞講演  2022.05 

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    Presentation type：Oral presentation (invited, special)  

  • 簡便にG-body形成が可能な低酸素培養系の確立

    伊藤里緒子, 村田有紀, 吉村柚紀, 平山伶奈, 内海亮太郎, 三浦夏子, 片岡道彦

    生物工学若手研究者の集い 夏のオンラインセミナー2022  2022.05 

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    Presentation type：Oral presentation (general)  

  • 「生命の不思議:細胞とMETA body 」 〜 酵素集合体形成による細胞の代謝制御 〜 Invited

    三浦夏子

    MPUFセミナー  2022.05 

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    Presentation type：Public lecture, seminar, tutorial, course, or other speech  

  • 細胞質内で形成される解糖系酵素集合体G-bodyとその制御 Invited

    三浦夏子

    JT医薬総合研究所講演会「相分離が支配する酵素反応系」  2022.04 

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    Presentation type：Symposium, workshop panel (nominated)  

  • 簡便に G body 形成が可能な低酸素培養系の確立

    伊藤里緒子, 村田有紀, 吉村柚紀, 平山伶奈, 内海亮太郎, 三浦夏子, 片岡道彦

    酵素工学研究会第８７回講演会  2022.04 

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    Presentation type：Poster presentation  

  • Development of in vitro hyperpolarized 13C NMR method for measurement of enzymatic reactions under hypoxia Invited

    2nd Metabolic Imaging Workshop in Gifu  2022.03 

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    Presentation type：Public lecture, seminar, tutorial, course, or other speech  

  • Metabolic regulation through spatial reorganization of metabolic enzymes Invited Domestic conference

    Natsuko Miura

    The 2022 Annual Meeting of The Japan Society for Bioscience, Biotechnology and Agrochemistry  2022.03 

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    Presentation type：Symposium, workshop panel (nominated)  

  • 代謝酵素群の集合体形成による細胞内代謝の調節 Invited

    三浦夏子

    META body研究会 第1回セミナー  2022.03 

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    Presentation type：Public lecture, seminar, tutorial, course, or other speech  

  • 生体内の代謝反応をリアルタイムにみる

    三浦夏子

    第3 回ACT-X 生命と化学 勉強会  2022.02 

  • Condensate Formation by Glycolytic Enzymes To Alter Carbon Metabolism under Hypoxia. Invited

    Natsuko Miura

    RIKEN CSRS seminar  2022.01 

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    Presentation type：Public lecture, seminar, tutorial, course, or other speech  

  • 低酸素環境下における酵素反応のin silico/in vitroリアルタイム測定系構築に関する検討 Domestic conference

    三浦夏子

    量子生命科学先端フォーラム 2021冬の研究会  2021.12 

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    Presentation type：Poster presentation  

  • 低酸素条件下における代謝酵素集合体“G-body"形成メカニズムの解明と制御に向けて Invited Domestic conference

    三浦夏子

    第44 回⽇本分⼦⽣物学会年会ワークショップ1PWS2-07「液-液相分離の酵素連続反応に対する効果」  2021.12 

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    Presentation type：Poster presentation  

  • 低酸素条件下における代謝酵素集合体“G-body”の形成 Invited Domestic conference

    三浦 夏子

    第94回日本生化学会大会シンポジウム3S11a「相分離メガネを通して⾒た酵素の機能」  2021.11 

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    Presentation type：Poster presentation  

  • カリケアミシン添加によるG-body形成への影響 Domestic conference

    園田啓吾, 三浦夏子, 片岡道彦

    生物工学若手研究者の集い 夏のオンラインセミナー2021  2021.07 

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    Presentation type：Oral presentation (general)  

  • ジャーファーメンターでの低酸素培養におけるG-body形成のための通気条件の検討 Domestic conference

    村田有紀, 三浦夏子, 片岡道彦

    2021年度生物工学若手研究者の集いオンラインセミナー  2021.07 

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    Presentation type：Oral presentation (general)  

  • 海洋由来の生分解性プラスチック分解菌の探索とその特性評価 Domestic conference

    小林 慶太, 三浦 夏子, 片岡 道彦

    第21回マリンバイオテクノロジー学会大会   2021.05 

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    Presentation type：Poster presentation  

  • Regulation of G-body formation by Saccharomyces cerevisiae enolase International conference

    Miura N, Hirayama R, Yoshimura Y, Utsumi R, Ueda M, Kataoka M

    Oxidative Stress and Disease Gordon Research Conference  2019.03 

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    Presentation type：Poster presentation  

  • アザミサンゴGalaxea fascicularisから単離したRuegeria属細菌の解析 Domestic conference

    三浦夏子, 岡田圭以子, 元根啓佑, 北村瑠璃子, 高木俊幸, 植田充美, 片岡道彦

    日本農芸化学会2019年度大会  2019.03 

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    Presentation type：Poster presentation  

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Grant-in-Aid for Scientific Research

  • Bottom-up creation of cell-free molecular systems: surpassing nature

    Grant-in-Aid for Transformative Research Areas (A)  2025

  • 低酸素下で形成される動的な代謝酵素集合体METAbody制御仮説の構築

    Grant-in-Aid for Scientific Research(B)  2025

  • 低酸素下で形成される動的な代謝酵素集合体METAbody制御仮説の構築

    Grant-in-Aid for Scientific Research(B)  2024

  • Bottom-up creation of cell-free molecular systems: surpassing nature

    Grant-in-Aid for Transformative Research Areas (A)  2024

  • 低酸素下で形成される動的な代謝酵素集合体METAbody制御仮説の構築

    Grant-in-Aid for Scientific Research(B)  2023

  • 天然で起こりえない進化を実現する無細胞分子システムのボトムアップ構築

    学術変革領域研究（A）  2023

  • 低酸素下で形成される動的な代謝酵素集合体METAbody制御仮説の構築

    Grant-in-Aid for Scientific Research(B)  2022

  • 天然で起こりえない進化を実現する無細胞分子システムのボトムアップ構築

    学術変革領域研究(A)  2022

  • 天然で起こりえない進化を実現する無細胞分子システムのボトムアップ構築

    学術変革領域研究(A)  2021

  • 低酸素下で形成される動的な代謝酵素集合体METAbody制御仮説の構築

    Grant-in-Aid for Scientific Research(B)  2021

  • サンゴ常在菌が産生するサンゴ菌叢維持分子Homeostaticsの同定

    Grant-in-Aid for Early-Career Scientists  2020

  • 酵素群の細胞内集合による代謝制御機序の解明

    戦略的創造研究推進事業 ACT-X「生命と化学」  2020

  • 量子技術を用いた超高感度MRI/NMR（公立大学法人大阪）

    光・量子飛躍フラッグシッププログラム（Q-LEAP）  2020

  • 細胞間コミュニケーション分子の生合成と代謝から迫る青枯病菌クオラムセンシング機構

    Grant-in-Aid for Scientific Research(B)  2019

  • サンゴ常在菌が産生するサンゴ菌叢維持分子Homeostaticsの同定

    Grant-in-Aid for Early-Career Scientists  2019

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Contract research

  • 細胞内外における代謝反応の定量的理解

    国立研究開発法人科学技術振興機構  【JST】革新的GX技術創出事業（GteX）  2025

  • 細胞の代謝を操作するMETAbody 制御ツールの開発

    国立研究開発法人科学技術振興機構  【JST】戦略的創造研究推進事業　さきがけ  2025

  • 量子技術を用いた超高感度MRI/NMR（公立大学法人大阪）

    国立研究開発法人量子科学技術研究開発機構  【文科省】国家課題対応型研究開発推進事業　光・量子飛躍フラッグシッププログラム（Q-LEAP）  2025

Charge of on-campus class subject

  • 生命機能化学概論

    2026     Undergraduate

  • 生命機能化学特論B

    2026     Graduate school

  • 生命機能化学研究プレゼンテーション

    2026     Graduate school

  • 発酵微生物学実験

    2026   Practical Training   Undergraduate

  • 発酵制御化学特論

    2026   Weekly class   Graduate school

  • 生物代謝制御学

    2026   Weekly class   Undergraduate

  • 生物代謝制御学

    2025   Weekly class   Undergraduate

  • 微生物学実験

    2025   Practical Training   Undergraduate

  • 基礎化学実験

    2025   Practical Training   Undergraduate

  • 生命機能化学特論B

    2025   Weekly class   Graduate school

  • 生命機能化学研究プレゼンテーション

    2025     Graduate school

  • 発酵制御化学特論

    2025   Weekly class   Graduate school

  • 生命機能化学概論

    2025   Weekly class   Undergraduate

  • 発酵微生物学実験

    2025   Weekly class   Undergraduate

  • 生命機能化学特論B

    2024   Weekly class   Graduate school

  • 生命機能化学研究プレゼンテーション

    2024   Intensive lecture   Graduate school

  • 発酵制御化学特論

    2024   Weekly class   Graduate school

  • 生物代謝制御学

    2024   Weekly class   Undergraduate

  • 初年次ゼミナール

    2024   Weekly class   Graduate school

  • 生命機能化学概論

    2024   Weekly class   Undergraduate

  • 発酵微生物学実験

    2024   Weekly class   Undergraduate

  • 微生物学実験

    2024   Practical Training   Undergraduate

  • 生命機能化学特論B

    2023   Weekly class   Graduate school

  • 生命機能化学研究プレゼンテーション

    2023   Intensive lecture   Graduate school

  • 発酵制御化学特論

    2023   Weekly class   Graduate school

  • 基礎化学実験

    2023   Weekly class   Graduate school

  • 生物代謝制御学

    2023   Weekly class   Undergraduate

  • 微生物学実験

    2023   Weekly class   Undergraduate

  • 生命機能化学概論

    2023   Weekly class   Undergraduate

  • 食品衛生学実習

    2023   Intensive lecture   Undergraduate

  • 生命機能化学研究プレゼンテーション

    2022   Intensive lecture   Graduate school

  • 発酵制御化学特論

    2022   Weekly class   Graduate school

  • 生命機能化学概論

    2022   Weekly class   Undergraduate

  • 微生物学実験

    2022   Weekly class   Undergraduate

  • 生物代謝制御学

    2022   Weekly class   Undergraduate

  • Laboratory : Microbiology

    2021   Practical Training  

  • General Principles of Applied Biological Chemistry

    2021    

  • Metabolic Regulation and Engineering

    2021    

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