Updated on 2024/03/14

写真a

 
KOYAMA Keisuke
 
Organization
Graduate School of Veterinary Science Department of Veterinary Science Associate Professor
School of Veterinary Science Department of Veterinary Science
Title
Associate Professor
Affiliation
Institute of Veterinary Science
Contact information
メールアドレス
Affiliation campus
Rinku Campus

Position

  • Graduate School of Veterinary Science Department of Veterinary Science 

    Associate Professor  2022.04 - Now

  • School of Veterinary Science Department of Veterinary Science 

    Associate Professor  2022.04 - Now

Degree

  • 博士(獣医学) ( Hokkaido University )

Research Areas

  • Life Science / Veterinary medical science

  • Life Science / Animal life science

  • Life Science / Animal production science

Research Interests

  • 臨床繁殖

  • 繁殖生理

  • 卵子成熟培養

  • 受胎率予測

  • 機械学習

  • 牛群検定

  • 卵巣予備能

Research subject summary

  • 個別培養系および人工知能を活用したウシ卵子体外培養系の最適化

  • 牛群検定成績に基づく受胎率予測

  • ホルスタイン種牛の妊娠期における卵巣予備維持機構

Professional Memberships

  • THE JAPANESE SOCIETY OF VETERINARY SCIENCE

      Domestic

  • JAPAN VETERINARY MEDICAL ASSOCIATION

      Domestic

  • SOCIETY FOR REPRODUCTION AND DEVELOPMENT

      Domestic

  • Japan Embryo Transfer Society

      Domestic

  • JAPANESE SOCIETY OF ANIMAL SCIENCE

      Domestic

  • 北海道牛受精卵移植研究会

      Domestic

  • 獣医疫学会

      Domestic

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Awards

  • 奨励賞

    古山敬祐, 山野紘輔, 川手憲俊, 大和田勇人

    2022.08   日本産業動物獣医学会(近畿)  

  • 奨励賞

    古山敬祐, 窪友瑛, 青木亜紀子, 市川雄紀, 閏間英之, 可知正行, 川本友香, 田村洋次, 小島秀介, 松尾麻未

    2021.10   日本産業動物獣医学会(近畿)  

  • 獣医繁殖学・優秀発表賞

    窪 友瑛, 古山敬祐, 堂腰顕, 田辺智樹, 松井義貴, 陰山聡一, 大和田勇人

    2021.09   日本獣医学会  

  • 優良賞

    古山敬祐, 小山毅, 窪友瑛, 杉本昌仁

    2018.10   北海道家畜人工授精師協会  

  • 北海道獣医師会長賞

    古山敬祐, 小山毅, 窪友瑛, 杉本昌仁, 横田修, 大和田勇人

    2018.09   北海道獣医師会  

  • 統計処理賞

    古山敬祐, 小山毅, 松井義貴, 昆野大次, 草刈直仁, 大坂郁夫

    2014.10   北海道家畜人工授精師協会  

  • スライド賞

    古山敬祐, 小山毅, 松井義貴, 大坂郁夫

    2013.10   北海道家畜人工授精師協会  

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Job Career (off-campus)

  • 北海道立総合研究機構 酪農試験場   研究主任

    2018.07 - 2019.03

  • 北海道立総合研究機構 根釧農業試験場   研究主任

    2016.01 - 2018.06

  • 北海道立総合研究機構 根釧農業試験場   研究職員

    2011.04 - 2015.12

Papers

  • Predicting nuclear maturation speed of oocytes from Japanese Black beef heifers through non-invasive observations during IVM: An attempt using machine learning algorithms. Reviewed

    Ho TC-T, Kawate N, Koyama K.

    Theriogenology   209   235 - 242   2023.07( ISSN:0093-691X

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    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Nuclear maturation is an essential process in which oocytes acquire the competence to develop further. However, the time required for nuclear maturation during IVM varies among oocytes. Therefore, predicting nuclear maturation speed (NMS) could help identify the optimal timing for IVF and maximize the developmental competence of each oocyte. This study aimed to establish machine learning-based prediction models for NMS using non-invasive indicators during the individual IVM of Japanese Black (JB) beef heifer oocytes. We collected ovaries from abattoirs and aspirated cumulus-oocyte complexes (COCs) from follicles with diameters ranging between 2 and 8 mm. The COCs were matured individually for 18 h, and photographs of each COC were taken at the beginning and every 3 h from 12 h to the end of maturation. After IVM culture, we denuded COCs and stained oocytes to confirm the progression of meiosis. Only oocytes that reached the metaphase II (MII) stage were considered to have a fast NMS. Morphological features, including COC area, cumulus expansion ratio, expansion rate per hour, and expansion pattern, were extracted from the recorded photos and applied to develop prediction models for NMS using machine learning algorithms. The MII rates of oocytes with fast- and slow-predicted NMS differed when the decision tree (DT) and random forest (RF) models were employed (P < 0.05). To evaluate the relationship between predicted NMS by DT and RF models and fertilization dynamics during individual IVF, sperm penetration and pronuclear formation were evaluated at 3, 6, 9, and 12 h after IVF start, following 24 h of IVM. The association between predicted NMS and embryo development was investigated by performing IVC for seven days using microwell culture dishes after 24 h of IVM and 6 h of IVF. Predicted NMS did not show a significant association with fertilization dynamics. However, oocytes with fast-predicted NMS by the RF model exhibited a tendency towards a higher cleavage rate 48 h after IVF start (P = 0.08); no other relationship was found between predicted NMS and embryo development. These findings demonstrate the feasibility of using non-invasive indicators during IVM to develop prediction models for NMS of JB beef heifer oocytes. Although the effect of predicted NMS on embryo development remains unclear, customized treatments based on NMS predictions have the potential to improve the efficiency of in vitro embryo production following individual IVM culture.

    DOI: 10.1016/j.theriogenology.2023.07.007

    PubMed

  • Parity affects the relationship between the insemination–ovulation interval and the conception rate in lactating dairy cows Reviewed

    Koyama K, Kubo T, Koyama T, Takahashi Y.

    Reprod Domest Anim   58 ( 7 )   895 - 902   2023.04( ISSN:0936-6768

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    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    The aim of the present study was to investigate the relationship between parity and the optimal insemination-ovulation interval (IOI) in lactating dairy cows, as well as the relationship between the optimal timing of artificial insemination (AI) based on the onset of estrus and parity. A total of 183 postpartum lactating Holstein cows participated in the study and underwent 522 AI procedures using unsorted frozen semen between 2011 and 2018. Transrectal ultrasound was used to detect ovulation times in 326 AI procedures, and a pedometer was used to detect estrus onset times in 470 AI procedures. An analysis was also performed on data from 265 AI procedures in which all the times of estrus onset, estrus end, and ovulation were detected. The results showed that ovulation occurring 6-12 hr after AI was associated with the numerical highest conception rate in multiparous cows. Moreover, the conception rate was higher when ovulation occurred between 6 and 12 hr after AI than when it occurred between 0 and 6 hr or 18 and 24 hr after AI. In contrast, the effect of IOI on the conception rate in primiparous cows was insignificant. There was no effect of AI timing based on the estrus onset on the conception rate in either primiparous or multiparous cows. Furthermore, the interval from estrus onset to ovulation was similar in primiparous and multiparous cows. The difference in optimal IOI between primiparous and multiparous cows may be due to the larger uterus size in multiparous cows. These findings suggest that the optimal timing for AI in multiparous cows is more restricted than in primiparous cows. Therefore, it is essential to carefully determine the timing of AI when it is performed in multiparous cows.

    DOI: 10.1111/rda.14363

    PubMed

  • Impaired placentomal interferon signaling as the possible cause of retained fetal membrane in parturition-induced cows Reviewed

    Inaba R, Kawahara-Miki R, Shinozawa A, Yasuhara T, Fujii T, Koyama K, Murata-Okubo M, Souma K, Hirayama H.

    J Reprod Dev   68 ( 1 )   30 - 37   2022.02( ISSN:0916-8818

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1262/jrd.2021-094

    PubMed

  • Relationship between sire predicted transmitting ability for daughter pregnancy rate and daughter's reproductive performance and milk production in Japanese dairy herds. Reviewed

    Koyama K, Takahashi T.

    J Reprod Dev   66 ( 5 )   445 - 452   2020.10( ISSN:0916-8818

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    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)   International / domestic magazine:Domestic journal  

    Modern genetic improvement in dairy cattle is directed towards improvement of fertility; however, reproduction traits generally exhibit a genetic antagonism with milk yield. Herein, we aimed to clarify the effects of sire predicted transmitting ability (PTA) for daughter pregnancy rate (DPR) on the reproductive performance and milk yield of daughters in Japanese dairy herds. We conducted a retrospective cohort study on four dairy herds in eastern Hokkaido, Japan, using 1,612 records from 1,018 cows with first, second, or third calvings between March 2015 and September 2018. First, we classified sires into three groups based on the tertile value of their DPR estimate: ≤ -2.2 (low), -2.1 to -0.4 (intermediate), and ≥ -0.3 (high). Subsequently, we compared the sire PTA estimates, reproductive performance, and milk production among DPR groups for each parity of the daughters. In the first and second parity, the hazard of pregnancy by 200 days postpartum was highest in cows from the high-DPR group (P < 0.05); in the third parity, it was unaffected by DPR group. Although sire PTA for milk production in cows from the low-DPR group was highest, actual milk production was unaffected by DPR group regardless of parity. Our findings demonstrate that using sires with PTA for high fertility can enable farmers to improve reproductive performance without decreasing milk yield in Japanese dairy herds. However, it should be noted that sires with PTA for high fertility are at risk for reducing the genetic merit for milk production.

    DOI: 10.1262/jrd.2020-048

    PubMed

  • Technical note: Calving prediction in dairy cattle based on continuous measurements of ventral tail base skin temperature using supervised machine learning. Reviewed

    Higaki S, Koyama K, Sasaki Y, Abe K, Honkawa K, Horii Y, Minamino T, Mikurino Y, Okada H, Miwakeichi F, Darhan H, Yoshioka K.

    J Dairy Sci   103 ( 9 )   8535 - 8540   2020.09( ISSN:0022-0302

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    In this study, we developed a calving prediction model based on continuous measurements of ventral tail base skin temperature (ST) with supervised machine learning and evaluated the predictive ability of the model in 2 dairy farms with distinct cattle management practices. The ST data were collected at 2- or 10-min intervals from 105 and 33 pregnant cattle (mean ± standard deviation: 2.2 ± 1.8 parities) reared in farms A (freestall barn, in a temperate climate) and B (tiestall barn, in a subarctic climate), respectively. After extracting maximum hourly ST, the change in values was expressed as residual ST (rST = actual hourly ST - mean ST for the same hour on the previous 3 d) and analyzed. In both farms, rST decreased in a biphasic manner before calving. Briefly, an ambient temperature-independent gradual decrease occurred from around 36 to 16 h before calving, and an ambient temperature-dependent sharp decrease occurred from around 6 h before until calving. To make a universal calving prediction model, training data were prepared from pregnant cattle under different ambient temperatures (10 data sets were randomly selected from each of the 3 ambient temperature groups: <15°C, ≥15°C to <25°C, and ≥25°C in farm A). An hourly calving prediction model was then constructed with the training data by support vector machine based on 15 features extracted from sensing data (indicative of pre-calving rST changes) and 1 feature from non-sensor-based data (days to expected calving date). When the prediction model was applied to the data that were not part of the training process, calving within the next 24 h was predicted with sensitivities and precisions of 85.3% and 71.9% in farm A (n = 75), and 81.8% and 67.5% in farm B (n = 33), respectively. No differences were observed in means and variances of intervals from the calving alerts to actual calving between farms (12.7 ± 5.8 and 13.0 ± 5.6 h in farms A and B, respectively). Above all, a calving prediction model based on continuous measurement of ST with supervised machine learning has the potential to achieve effective calving prediction, irrespective of the rearing condition in dairy cattle.

    DOI: 10.3168/jds.2019-17689

    PubMed

  • Expression of C-C motif chemokines and their receptors in bovine placentomes at spontaneous and induced parturition. Reviewed

    Hirayama H, Sakumoto R, Koyama K, Yasuhara T, Hasegawa T, Inaba R, Fujii T, Naito A, Moriyasu S, Kageyama S.

    J Reprod Dev   66 ( 1 )   49 - 55   2020.02( ISSN:0916-8818

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:Domestic journal  

    In bovine placentomes, the inflammatory response is considered important for the detachment of the fetal membrane from the caruncle after parturition. Glucocorticoids, a trigger of the onset of parturition, facilitate functional maturation of placentomes via prostaglandin (PG) and estrogen production in cattle. This study investigated how exogeneous glucocorticoids, which exert immunosuppressive effects, affect placental inflammation at parturition. Placentomes were collected immediately after spontaneous or induced parturition. Parturition was conventionally induced using PGF2α or dexamethasone or with a combination of triamcinolone acetonide and high-dose betamethasone (TABET treatment). Polymerase chain reaction (PCR) array analysis indicated that 9/13 C-C motif chemokine ligands (CCLs) were upregulated > two-fold in spontaneous parturition, with CCL2 and CCL8 being highly expressed. The expressions of CCL2, CCL8, C-C motif chemokine receptor 1 (CCR1), and CCR5 in caruncles were significantly higher in spontaneous parturition than in induced parturition. Although the clinical dose of dexamethasone did not influence the expression of these CCLs and CCRs, TABET treatment increased CCR1 expression. CCL8, CCR1, CCR2, and CCR5 were localized in the caruncular epithelial cells. CCR2 was also localized in the epithelial cells of the cotyledonary villi. This study is the first report to reveal the disruption in CCL and CCR expression in bovine placentomes at induced parturition. Enhanced glucocorticoid exposure for the induction of parturition may upregulate CCR1 expression in placentomes, but the treatment does not adequately promote CCL expression. Additionally, immunohistochemistry suggested that the CCL-CCR system is involved in the functional regulation of maternal and fetal epithelial cells in placentomes at parturition.

    DOI: 10.1262/jrd.2019-113

    PubMed

  • Enhanced glucocorticoid exposure facilitates the expression of genes involved in prostaglandin and estrogen syntheses in bovine placentomes at induced parturition. Reviewed

    Yasuhara T, Koyama K, Sakumoto R, Fujii T, Naito A, Moriyasu S, Kageyama S, Hirayama H

    Theriogenology   139   1 - 7   2019.07( ISSN:0093-691X

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    The mechanism by which the fetal membrane detaches after parturition in cattle is poorly understood, but the upregulation of placentomal prostaglandin and estrogen synthesis are considered to be important. This study investigated whether enhanced glucocorticoid exposure affected the functional maturation of placentomes at induced parturition. Placentomes were collected immediately after spontaneous (beef; n = 5, dairy; n = 5) or induced parturition in beef and dairy cattle. Parturition was induced conventionally using prostaglandin F2α (beef; n = 7, dairy; n = 6) or dexamethasone (beef; n = 6) or with a combination of triamcinolone acetonide (a long-acting glucocorticoid) and a high dose of betamethasone (TABET treatment, beef; n = 6, dairy; n = 9). Gene expression levels and protein localization in placentomes were analyzed by RT-qPCR and immunohistochemistry, respectively. Compared with the conventional methods, TABET treatment resulted in upregulated PTGS2 expression in cotyledons. The expression levels of PTGS2 and PGES were positively correlated in both cotyledons and caruncles. TABET treatment also upregulated the expression of CYP17A1, but not of CYP19A1, in cotyledons. The results revealed, for the first time, that PLA2G4A was localized in microvascular endothelial cells in the cotyledonary villi and the maternal septum. PTGS2 and PGES were colocalized in mononucleated cells of the cotyledonary villi and caruncle epithelial cells adjacent to the chorionic plate. TABET treatment upregulated the expression of placentomal genes involved in PGE2 synthesis and the conversion of pregnenolone to androstenedione. Thus, enhanced glucocorticoid exposure might partially facilitate the functional maturation of placentomes at induced parturition in cattle.

    DOI: 10.1016/j.theriogenology.2019.07.016

    PubMed

  • Prediction of calving time in Holstein dairy cows by monitoring the ventral tail base surface temperature Reviewed

    Koyama K, Koyama T, Sugimoto M, Kusakari N, Miura R, Yoshioka K, Hirako M.

    Vet J   240   1 - 5   2018.10( ISSN:1090-0233

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    Authorship:Lead author   Kind of work:Joint Work   International / domestic magazine:International journal  

  • Repeatability of antral follicle count according parity in dairy cows. Reviewed

    Koyama K, Koyama T, Sugimoto M

    J Reprod Dev   64 ( 6 )   535 - 539   2018.10( ISSN:0916-8818

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    Authorship:Lead author, Corresponding author   Kind of work:Joint Work   International / domestic magazine:International journal  

  • Optimization of diagnostic methods and criteria of endometritis for various postpartum days to evaluate infertility in dairy cows. Reviewed

    Koyama T, Omori R, Koyama K, Matsui Y, Sugimoto M

    Theriogenology   119 ( 1 )   225 - 232   2018.10( ISSN:0093-691X

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    Kind of work:Joint Work   International / domestic magazine:International journal  

  • Hot topic: Pregnancy-induced expression of interferon-stimulated genes in the cervical and vaginal mucosal membranes. Reviewed

    Kunii H, Koyama K, Ito T, Suzuki T, Balboula AZ, Shirozu T, Bai H, Nagano M, Kawahara M

    J Dairy Sci   101 ( 9 )   8396 - 8400   2018.09( ISSN:0022-0302

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    Kind of work:Joint Work   International / domestic magazine:International journal  

  • Characteristics of dairy cows with a pronounced reduction in first milk yield after estrus onset Reviewed

    Koyama K, Koyama T, Matsui Y, Sugimoto M, Kusakari N, Osaka I, Nagano M

    Jpn J Vet Res   65 ( 2 )   55 - 63   2017.05( ISSN:0047-1917

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    Authorship:Lead author, Corresponding author   Kind of work:Joint Work  

  • Early germinal vesicle breakdown is a predictor of high preimplantation developmental competent oocytes in mice. Reviewed

    Higaki S, Kishi M, Koyama K, Nagano M, Katagiri S, Takada T, Takahashi Y

    Zygote   25 ( 1 )   41 - 48   2017.02( ISSN:0967-1994

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    Kind of work:Joint Work   International / domestic magazine:International journal  

  • Localization of TGF-β and TGF-β receptor in bovine term placentome and expression differences between spontaneous and induced parturition. Reviewed

    Hirayama H, Koyama K, Sawai K, Fujii T, Naito A, Fukuda S, Kageyama S

    Placenta   36 ( 11 )   1239 - 1245   2015.11( ISSN:0143-4004

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    Kind of work:Joint Work   International / domestic magazine:International journal  

  • The efficacy of the well of the well (WOW) culture system on development of bovine embryos in a small group and the effect of number of adjacent embryos on their development. Reviewed

    Kang SS, Ofuji S, Imai K, Huang W, Koyama K, Yanagawa Y, Takahashi Y, Nagano M

    Zygote   23 ( 3 )   412 - 415   2015.06( ISSN:0967-1994

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    Kind of work:Joint Work   International / domestic magazine:International journal  

  • Addition of D-penicillamine, hypotaurine, and epinephrine (PHE) mixture to IVF medium maintains motility and longevity of bovine sperm and enhances stable production of blastocysts in vitro. Reviewed

    Kang SS, Koyama K, Huang W, Yang Y, Yanagawa Y, Takahashi Y, Nagano M

    J Reprod Dev   61 ( 2 )   99 - 105   2015.04( ISSN:0916-8818

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    Kind of work:Joint Work   International / domestic magazine:Domestic journal  

  • Effects of ovarian storage condition on in vitro maturation of Hokkaido sika deer (Cervus nippon yesoensis) oocytes. Reviewed

    Tulake K, Yanagawa Y, Takahashi Y, Katagiri S, Higaki S, Koyama K, Wang X, Li H

    Jpn J Vet Res   62 ( 4 )   187 - 192   2014.11( ISSN:0047-1917

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    Kind of work:Joint Work   International / domestic magazine:Domestic journal  

  • Estimation of the optimal timing of fertilization for embryo development of in vitro-matured bovine oocytes based on the times of nuclear maturation and sperm penetration. Reviewed

    Koyama K, Kang SS, Huang W, Yanagawa Y, Takahashi Y, Nagano M

    J Vet Med Sci   76 ( 5 )   653 - 659   2014.05( ISSN:0916-7250

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    Authorship:Lead author   Kind of work:Joint Work   International / domestic magazine:Domestic journal  

  • Enhancement of maternal recognition of pregnancy with parthenogenetic embryos in bovine embryo transfer. Reviewed

    Hirayama H, Moriyasu S, Kageyama S, Sawai K, Takahashi H, Geshi M, Fujii T, Koyama T, Koyama K, Miyamoto A, Matsui M, Minamihashi A

    Theriogenology   81 ( 8 )   1108 - 1115   2014.05( ISSN:0093-691X

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    Kind of work:Joint Work   International / domestic magazine:International journal  

  • Aging-related changes in in vitro-matured bovine oocytes: oxidative stress, mitochondrial activity and ATP content after nuclear maturation. Reviewed

    Koyama K, Kang SS, Huang W, Yanagawa Y, Takahashi Y, Nagano M

    J Reprod Dev   60 ( 2 )   136 - 142   2014.04( ISSN:0916-8818

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    Authorship:Lead author   Kind of work:Joint Work   International / domestic magazine:International journal  

  • In vitro maturation system for individual culture of bovine oocytes using micro-volume multi-well plate. Reviewed

    Nagano M, Kang SS, Koyama K, Huang W, Yanagawa Y, Takahashi Y

    Jpn J Vet Res   61 ( 4 )   149 - 154   2013.11( ISSN:0047-1917

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    Kind of work:Joint Work   International / domestic magazine:Domestic journal  

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Books and Other Publications

  • 新しい子牛の科学: 胎生期から初産分娩まで(第6章 初回妊娠から分娩までの生理と管理 6-1 初産牛の分娩生理)

    堂地修、古山敬祐( Role: Joint author)

    緑書房   2021.03 

MISC

  • 211 Postponed in vitro fertilization timing improves cleavage competence in oocytes with slow-predicted nuclear maturation speed in Japanese Black beef heifers Reviewed

    T. C. Ho, N. Kawate, K. Koyama

    Reproduction, Fertility and Development   36 ( 2 )   261 - 261   2023.12( ISSN:1031-3613 ( eISSN:1448-5990

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    Authorship:Last author, Corresponding author   Publishing type:Research paper, summary (international conference)  

    DOI: 10.1071/rdv36n2ab211

  • Ovarian origins and cumulus expansion pattern affected maturation and subsequent embryo development for in vitro production of bovine oocytes

    Thomas Chia-Tang Ho, Noritoshi Kawate, Keisuke Koyama

    日本繁殖生物学会第116回大会   2023.09

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  • ホルスタイン種雌牛における育成期の血中抗ミューラー管ホルモン濃度動態

    窪 友瑛, 新宮 裕子, 田辺 智樹, 若槻 拓司, 細字 晴仁, 古山 敬祐

    日本畜産学会大会講演要旨集   131回   143 - 143   2023.09( ISSN:1342-4688

  • ホルスタイン種泌乳牛の妊娠期における血中抗ミューラー管ホルモン動態

    HO Thomas Chia-Tang, 保田茂人, 窪友瑛, 川手憲俊, 古山敬祐

    日本産業動物獣医学会(近畿)・日本小動物獣医学会(近畿)・日本獣医公衆衛生学会(近畿)   2023   2023

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  • The mechanism for keeping the ovarian reserve level during the pregnancy period in Holstein cows

    古山敬祐, 窪友瑛

    食肉に関する助成研究調査成果報告書   41   2023

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  • 臨床獣医師のための研究室ガイド【第59回】獣医繁殖学の基礎から応用研究まで取り組み、飼育動物の繁殖の「なぜ?」に向き合う Invited

    川手憲俊, 古山敬祐

    臨床獣医   40 ( 10 )   74 - 76   2022.09

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    Publishing type:Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)  

  • 乾乳後期におけるルーメンバイパスアミノ酸製剤給餌効果の異なる2酪農場の比較

    古山敬祐, 山野紘輔, 川手憲俊, 大和田勇人

    令和4年度獣医学術近畿地区学会   2022.08

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  • 黒毛和種における黄体の大きさの調査

    秋山 智香, 小山 毅, 櫻井 直樹, 堀川 盟夫, 古山 敬祐

    繁殖技術   42 ( 4 )   101 - 103   2022.07( ISSN:0017-7520

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    Publishing type:Research paper, summary (national, other academic conference)  

  • ワンポイント質問 ウシにおける薬剤を用いた流産

    古山 敬祐

    家畜診療   69 ( 4 )   232 - 235   2022.04( ISSN:0287-0754

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    Publishing type:Article, review, commentary, editorial, etc. (other)  

  • 【正確な検査結果のための現場におけるサンプリング】サイトブラシを用いた牛子宮内膜炎の診断

    古山 敬祐

    臨床獣医   40 ( 4 )   30 - 34   2022.04( ISSN:0912-1501

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    Publishing type:Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)  

  • ホルスタイン種泌乳牛群における社会的順位と発情行動および採食行動との関連性

    窪友瑛, 古山敬祐, 堂腰顕, 田辺智樹, 松井義貴, 陰山聡一, 大和田勇人

    日本獣医学会学術集会講演要旨集   164th (CD-ROM)   2021( ISSN:1347-8621

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    Publishing type:Research paper, summary (national, other academic conference)  

    J-GLOBAL

  • 高受胎率であると予測されたホルスタイン種泌乳牛の栄養代謝状態および繁殖性の精査

    窪友瑛, 小山毅, 田辺智樹, 松井義貴, 堂腰顕, 古山敬祐

    繁殖技術   41 ( 6 )   2021( ISSN:0017-7520

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    Publishing type:Research paper, summary (national, other academic conference)  

    J-GLOBAL

  • 泌乳牛における黄体内腔の存在と受胎性および黄体機能との関係

    窪友瑛, 古山敬祐, 田辺智樹, 松井義貴, 堂腰顕, 陰山聡一

    日本胚移植学雑誌   42 ( 3 )   2021( ISSN:1341-2965

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  • ホルスタイン種牛における第一卵胞波主席卵胞の位置と適期授精時の受胎との関連性に及ぼす血中性ステロイドホルモン濃度の影響

    古山敬祐, 窪友瑛, 青木亜紀子, 青木亜紀子, 市川雄紀, 閏間英之, 可知正行, 可知正行, 川本友香, 川本友香, 田村洋次, 小島秀介, 松尾麻未, 松尾麻未

    日本産業動物獣医学会(近畿)・日本小動物獣医学会(近畿)・日本獣医公衆衛生学会(近畿)   2021   2021

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  • ワンポイント質問 ウシにおける薬剤を用いた分娩誘起

    古山 敬祐

    家畜診療   67 ( 11 )   647 - 651   2020.11( ISSN:0287-0754

  • 種雄牛のDPRと娘牛の繁殖成績および乳量との関連性

    高橋 孝嘉, 長崎 浩二, 友貞 直, 佐々木 尚洋, 桂 百貴, 山口 輝介, 郡司 祐貴, 丹羽 永昌, 林 侑太, 山口 大輝, 岩瀬 奨, 古山 敬祐

    繁殖技術   40 ( 6 )   140 - 142   2020.11( ISSN:0017-7520

  • ホルスタイン種牛における第一卵胞波主席卵胞の位置と人工授精の受胎との関連性解析

    古山 敬祐, 窪 友瑛, 青木 亜紀子, 市川 雄紀, 閏間 英之, 可知 正行, 川本 友香, Ho Chia-Tang, 川手 憲俊

    日本獣医学会学術集会講演要旨集   163回   267 - 267   2020.10( ISSN:1347-8621

  • 乳牛における分娩後ルーメン内LPS濃度の上昇が血液生化学性状および繁殖成績に及ぼす影響

    小田川 陽, 大滝 忠利, 古山 敬祐, 大矢 琴美, 梶川 博, 住吉 俊亮, 浅野 早苗, 堀北 哲也

    日本獣医学会学術集会講演要旨集   163回   265 - 265   2020.10( ISSN:1347-8621

  • Interferon signal activation in the placental node during bovine calving

    INABA Ryo, YASUHARA Taichi, KAWAHARA Ryouka, SHINOZAWA Akihisa, FUZII Takashi, NAITO Akira, KOYAMA Keisuke, SAKUMOTO Ryosuke, HIRAYAMA Hiroki

    The Journal of Reproduction and Development Supplement   113 ( 0 )   P - 73-P-73   2020

  • 機械学習を用いた牛用尾部体表温センサデータによる発情検知・分娩予測

    檜垣彰吾, 吉岡耕治, 鈴木千恵, 櫻井玲奈, 岡田浩尚, 古山敬祐, 佐々木羊介, 阿部剛大, 本川和幸, 南野知也, 三栗野陽子, 堀井洋一郎

    農研機構動物衛生研究部門成果情報(Web)   2020   2020

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  • Direct involvement of IFNT in the stimulation of ISGs in pregnant bovine cervical tissue

    KUNII Hiroki, KUBO Tomoaki, ASAOKA Natsuki, SHIMASAKI Tomoya, KOYAMA Keisuke, KIMURA Koji, BAI Hanako, KAWAHARA Manabu, TAKAHASHI Masashi

    The Journal of Reproduction and Development Supplement   113 ( 0 )   P - 65-P-65   2020

  • Detection of Pregnant Down-regulated Gene in Bovine Cervical Mucosa

    SHIMASAKI Tomoya, TAKAHASHI Masashi, KUBO Tomohide, KUNII Hiroki, HURUYAMA Keisuke, HAMAGUCHI Yu, ASAOKA Natsuki, BAI Hanako, KAWAHARA Manabu, OGAWA Hidehiko

    The Journal of Reproduction and Development Supplement   113 ( 0 )   P - 66-P-66   2020

  • 粗飼料多給飼養環境下の乳牛における分娩後ルーメン内LPS濃度の変動と繁殖成績との関連性

    大矢琴美, 大滝忠利, 古山敬祐, 梶川博, 住吉俊亮, 浅野早苗, 堀北哲也

    日本獣医学会学術集会講演要旨集   162nd   437   2019.08( ISSN:1347-8621

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  • 現場で活かす酪農技術 繁殖 X精液受胎率の予測可能性に迫る 牛群検定成績の活用 Invited

    古山敬祐

    Dairy Japan   64 ( 10 )   16‐19   2019.08

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  • 「畜産におけるICTを活用した取り組み」家畜センシング

    古山敬祐

    北海道畜産草地学会報   7   67‐69   2019.03( ISSN:2187-5391

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  • ホルスタイン種乳牛における体表温及び活動量に基づく機械学習による分娩時刻予測

    古山敬祐, 小山毅, 窪友瑛, 杉本昌仁, 檜垣彰吾, 吉岡耕治

    日本獣医師会獣医学術学会年次大会講演要旨集   2018   183   2019.01

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  • Pregnancy-specific expression of <i>IFIT1</i> in the bovine external uterine mucosa

    ASAOKA Natsuki, KIMURA Koji, TAKAHASHI Masashi, KUNII Hiroki, KOYAMA Keisuke, KUBO Tomoaki, HAMAGUCHI Yu, OGAWA Hidehiko, KOBAYASHI Hisato, BAI Hanako, KAWAHARA Manabu

    The Journal of Reproduction and Development Supplement   112 ( 0 )   P - 71-P-71   2019( ISSN:0916-8818

  • 生乳中P4濃度に基づく乳牛の分娩後卵巣周期回復日と繁殖成績および乳量・乳成分との関連性

    古山敬祐, 小山毅, 窪友瑛, 杉本昌仁, 横田修, 大和田勇人

    北海道獣医師会雑誌   2018.08

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  • 一牛群で見られた分娩後初回排卵日の周期的変化はなぜ起きたのか?

    古山敬祐, 小山毅, 草刈直仁, 杉本昌仁

    繁殖技術   2018.06

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  • シンポジウム講演 性選別精液の授精適期および新規発情検知システムの開発

    古山 敬祐

    繁殖技術   2018.03

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  • 現場で活かす酪農技術 繁殖管理 ホル種未経産牛 性選別精液の授精指針

    古山 敬祐

    Dairy Japan   2017.11

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  • 乳牛におけるウェアラブルセンサによる分娩および排卵予知

    古山 敬祐

    MPアグロジャーナル   2017.01

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  • ホルスタイン種育成牛における目視による発情発見に基づいた性選別凍結精液の人工授精適期

    古山敬祐, 小山毅, 松井義貴, 草刈直仁, 杉本昌仁, 早川宏之, 高橋芳幸

    繁殖技術   2016.12

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  • 現場で活かす酪農技術 繁殖管理“発情後出血の見られやすい牛”とはどういう牛か?牛群評価の指標としての発情後出血の見られる割合

    古山 敬祐

    Dairy Japan   2016.11

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  • 発情後出血の見られやすい牛とはどういう牛か?

    古山敬祐, 小山毅, 松井義貴, 草刈直仁, 杉本昌仁

    繁殖技術   2016.09

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  • 発情時に乳量は低下するのか? ホルスタイン種経産牛における調査

    古山 敬祐, 小山 毅, 松井 義貴, 昆野 大次, 草刈 直仁, 大坂 郁夫

    繁殖技術   2015.09

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  • 乳牛における発情開始直後の乳量低下に関わる要因

    古山 敬祐, 小山 毅, 松井 義貴, 杉本 昌仁, 草刈 直仁, 大坂 郁夫

    北海道獣医師会雑誌   2015.08

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  • 現場で活かす酪農技術 繁殖管理 発情後出血を見逃さないで!

    古山 敬祐

    Dairy Japan   2015.02

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  • 乳用経産牛における第一卵胞波主席卵胞と黄体の位置関係が受胎率および産子の性別に及ぼす影響

    古山 敬祐, 小山 毅, 松井 義貴, 大坂 郁夫, 草刈 直仁, 三浦 亮太朗, 松井 基純

    北海道獣医師会雑誌   2014.08

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  • ホルスタイン種経産牛での発情後出血の見られる時期および人工授精後の受胎率との関連

    古山 敬祐, 小山 毅, 松井 義貴, 大坂 郁夫

    繁殖技術   2014.03

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  • AM-PM法の有用性の検討

    中村 俊二, 古山 敬祐, 大坂 郁夫

    繁殖技術   2012.09

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  • 雌犬における局所摘出後の乳腺腫瘍続発率

    古山 敬祐

    北海道獣医師会雑誌   2008.09

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Presentations

  • 牛群検定成績に基づく受胎率予測 Invited Domestic conference

    古山 敬祐

    令和5年度北海道牛受精卵移植研究会シンポジウム  2024.02 

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    Presentation type:Symposium, workshop panel (nominated)  

    Venue:札幌市(北海道大学)  

  • 人工授精および受精卵移植における発情日以降に実施可能な受胎率改善方策 Invited Domestic conference

    古山 敬祐

    令和5年度家畜診療等技術近畿地区研修会  2023.11 

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    Presentation type:Public lecture, seminar, tutorial, course, or other speech  

  • 定時人工授精の基本と応用 Invited Domestic conference

    古山 敬祐

    「新規獣医師が基礎的な臨床技術を修得するための技術研修」(令和5年度獣医師能力向上・就業支援・地域獣医療体制整備支援事業)  2023.11 

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    Presentation type:Public lecture, seminar, tutorial, course, or other speech  

  • 牛の繁殖管理とセンシング技術 Invited

    古山敬祐

    一般社団法人ものづくり医療コンソーシアム  2023.02 

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    Presentation type:Public lecture, seminar, tutorial, course, or other speech  

  • 卵巣予備能に基づく高受胎性のウシづくり Invited

    古山 敬祐

    令和3年度中堅獣医師講習会(NOSAI協会)  2021.11 

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    Presentation type:Public lecture, seminar, tutorial, course, or other speech  

  • 定時人工授精に関する知見のアップデート Invited

    古山 敬祐

    令和3年度中堅獣医師講習会(NOSAI協会)  2021.11 

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    Presentation type:Public lecture, seminar, tutorial, course, or other speech  

  • 乳用牛繁殖管理におけるセンシング技術および先天的受胎性からのアプローチ Invited

    古山 敬祐

    令和2年度家畜診療等技術近畿地区研修会  2020.11 

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    Presentation type:Oral presentation (invited, special)  

  • 乳用牛繁殖予測技術の現状および展望 Invited Domestic conference

    古山 敬祐

    令和元年度京都府獣医師会産業動物部会研修会  2019.09 

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    Presentation type:Public lecture, seminar, tutorial, course, or other speech  

  • 「畜産におけるICTを活用した取り組み」 Invited Domestic conference

    古山敬祐

    北海道畜産草地学会  2019 

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    Presentation type:Oral presentation (invited, special)  

  • 牛のウエアラブル生体センシング技術について~体表温センサ~ Invited Domestic conference

    古山 敬祐

    オホーツク・スマート農業セミナー2018  2018 

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    Presentation type:Oral presentation (invited, special)  

  • ウシの卵巣・子宮の超音波画像から得られる情報 Invited Domestic conference

    古山 敬祐

    宮崎大学 中核的専門人材養成事業  2017 

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    Presentation type:Oral presentation (invited, special)  

  • シンポジウム講演 性選別精液の授精適期および新規発情検知システムの開発 Invited Domestic conference

    古山 敬祐

    北海道家畜人工授精師技術研修大会  2017 

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    Presentation type:Oral presentation (invited, special)  

  • 乳牛における体表温センサによる分娩予知および腟内センサによる排卵予知 Invited Domestic conference

    古山 敬祐

    動物用ワクチン-バイオ医薬品研究会シンポジウム  2016 

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    Presentation type:Oral presentation (invited, special)  

  • 性選別凍結精液の人工授精適期の検討について Invited Domestic conference

    古山 敬祐

    一般社団法人ジェネティクス北海道十勝北見事業所担当者会議  2015 

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    Presentation type:Oral presentation (invited, special)  

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Collaborative research (seeds) keywords

  • 乳牛の繁殖管理

    Category:Life Science 

  • 牛受精卵の体外培養

    Category:Life Science 

  • 牛卵子の体外培養

    Category:Life Science 

Grant-in-Aid for Scientific Research

  • 雌ウシのINSL3分泌動態および生殖器・生殖細胞における受容体発現と役割の解明

    Grant-in-Aid for Scientific Research(C)  2023

  • 高着床能力胚の生産を目指した核成熟速度に対応したウシ卵子体外培養系の構築

    Grant-in-Aid for Early-Career Scientists  2022

  • 高着床能力胚の生産を目指した核成熟速度に対応したウシ卵子体外培養系の構築

    Grant-in-Aid for Early-Career Scientists  2021

  • 高着床能力胚の生産を目指した核成熟速度に対応したウシ卵子体外培養系の構築

    Grant-in-Aid for Early-Career Scientists  2020

  • 乳牛の亜急性ルーメンアシドーシスによる繁殖機能低下要因の解明と代謝機能改善効果

    Grant-in-Aid for Scientific Research(C)  2019

  • 牛の分娩時胎盤節における炎症誘導機構の解明と胎盤成熟誘導型分娩誘起技術開発

    Grant-in-Aid for Scientific Research(B)  2019

  • 母体テストステロンを介した産子の卵巣予備能低下機構に関する研究

    Grant-in-Aid for Early-Career Scientists  2018

  • 乳牛個体間の社会的順位による発情発見アルゴリズムの開発

    Grant-in-Aid for Scientific Research(B)  2018

  • 乳牛の亜急性ルーメンアシドーシスによる繁殖機能低下要因の解明と代謝機能改善効果

    Grant-in-Aid for Scientific Research(C)  2018

  • 牛の分娩時胎盤節における炎症誘導機構の解明と胎盤成熟誘導型分娩誘起技術開発

    Grant-in-Aid for Scientific Research(B)  2018

  • 母体テストステロンを介した産子の卵巣予備能低下機構に関する研究

    Grant-in-Aid for Early-Career Scientists  2017

  • 乳牛の亜急性ルーメンアシドーシスによる繁殖機能低下要因の解明と代謝機能改善効果

    Grant-in-Aid for Scientific Research(C)  2017

  • 牛の分娩時胎盤節における炎症誘導機構の解明と胎盤成熟誘導型分娩誘起技術開発

    Grant-in-Aid for Scientific Research(B)  2017

  • 牛の分娩時胎盤節における炎症誘導機構の解明と胎盤成熟誘導型分娩誘起技術開発

    Grant-in-Aid for Scientific Research(B)  2016

  • 母体テストステロンを介した産子の卵巣予備能低下機構に関する研究

    Grant-in-Aid for Early-Career Scientists  2016

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Joint research

  • 受胎率予測による乳牛繁殖管理技術開発事業

    公益財団法人 全国競馬・畜産振興会  日本中央競馬会畜産振興事業  2023

  • ホルスタイン種牛の妊娠期における卵巣予備能維持機構

    地方独立行政法人北海道立総合研究機構  2022

Contract research

  • 令和3年度高泌乳牛の飼養・繁殖管理技術の高度化事業

    東京理科大  2021

  • 令和2年度高泌乳牛の飼養・繁殖管理技術の高度化事業

    学校法人東京理科大学  2020

  • 令和1年度高泌乳牛の飼養・繁殖管理技術の高度化事業

    学校法人東京理科大学  2019

Incentive donations / subsidies

  • ウシ卵⼦成熟培養時のグルコース消費量と成熟および発 ⽣能との関連性

    大阪公立大学  令和5(2023)年度 戦略的研究推進事業(若⼿研究者⽀援)  2023

  • ホルスタイン種牛の妊娠期における卵巣予備維持機構

    公益財団法人伊藤記念財団  2022

  • 内腔の有無に着目したウシ形成期黄体の機能解析

    公益財団法人伊藤記念財団  2021

Charge of on-campus class subject

  • 大動物野外診療実習

    2023   Intensive lecture   Undergraduate

  • 畜産学実習

    2023   Intensive lecture   Undergraduate

  • 小動物基礎臨床実習

    2023   Intensive lecture   Undergraduate

  • 獣医科学特別研究1

    2023   Intensive lecture   Graduate school

  • 畜産学実習

    2023   Intensive lecture   Undergraduate

  • 臨床基礎実習

    2023   Weekly class   Undergraduate

  • 大動物診療実習

    2023   Intensive lecture   Undergraduate

  • 大動物野外診療実習

    2023   Intensive lecture   Undergraduate

  • 産業動物生産獣医療

    2023   Weekly class   Undergraduate

  • 牧場実習

    2023   Intensive lecture   Undergraduate

  • 大動物臨床B

    2023   Intensive lecture   Undergraduate

  • 大動物臨床A

    2023   Intensive lecture   Undergraduate

  • 獣医臨床栄養学

    2023   Weekly class   Undergraduate

  • 獣医繁殖学A

    2023   Weekly class   Undergraduate

  • 初年次ゼミナール

    2023   Weekly class   Graduate school

  • 国際・国内インターンシップ

    2023   Intensive lecture   Graduate school

  • 獣医科学特別研究1

    2023   Intensive lecture   Graduate school

  • 獣医科学特別演習1

    2023   Intensive lecture   Graduate school

  • 獣医科学演習

    2023   Intensive lecture   Graduate school

  • 伴侶動物診療学特論

    2023   Intensive lecture   Graduate school

  • 生産獣医療学特論

    2023   Intensive lecture   Graduate school

  • 国際・国内インターンシップ

    2023   Intensive lecture   Graduate school

  • 獣医科学特別研究1

    2023   Intensive lecture   Graduate school

  • 獣医科学特別演習1

    2023   Intensive lecture   Graduate school

  • 獣医科学ミニレビュー

    2023   Intensive lecture   Graduate school

  • 獣医科学演習

    2023   Intensive lecture   Graduate school

  • 獣医科学特別研究1

    2023   Intensive lecture   Graduate school

  • 獣医体験演習

    2023   Weekly class   Undergraduate

  • 獣医繁殖学B

    2023   Weekly class   Undergraduate

  • 獣医繁殖学実習

    2023   Weekly class   Undergraduate

  • 生物学実験

    2023   Intensive lecture   Graduate school

  • 小動物基礎臨床実習

    2022   Intensive lecture   Undergraduate

  • 獣医科学特別演習1

    2022   Intensive lecture   Graduate school

  • 産業動物生産獣医療

    2022   Weekly class   Undergraduate

  • 獣医臨床栄養学

    2022   Weekly class   Undergraduate

  • 獣医繁殖学A

    2022   Weekly class   Undergraduate

  • 獣医科学特別演習1

    2022   Intensive lecture   Graduate school

  • 伴侶動物診療学特論

    2022   Intensive lecture   Graduate school

  • 国際・国内インターンシップ

    2022   Intensive lecture   Graduate school

  • 獣医科学特別研究1

    2022   Intensive lecture   Graduate school

  • 獣医繁殖学B

    2022   Weekly class   Undergraduate

  • 獣医繁殖学実習

    2022   Weekly class   Undergraduate

  • 大動物臨床A

    2022   Intensive lecture   Undergraduate

  • 大動物臨床B

    2022   Intensive lecture   Undergraduate

  • 牧場実習

    2022   Intensive lecture   Undergraduate

  • 大動物野外診療実習

    2022   Intensive lecture   Undergraduate

  • 大動物診療実習

    2022   Intensive lecture   Undergraduate

  • 臨床基礎実習

    2022   Weekly class   Undergraduate

  • 獣医体験演習

    2022   Weekly class   Undergraduate

  • 畜産学実習

    2022   Intensive lecture   Undergraduate

  • 食料生産実習

    2022   Intensive lecture   Undergraduate

  • 生物学実験獣医

    2022   Intensive lecture   Graduate school

  • 食料生産実習

    2022   Intensive lecture   Undergraduate

  • Fundamental Experiments of Biology

    2021   Practical Training  

  • Special Lecture: Advanced Pathobiology C

    2021    

  • Practical Training for Food  production

    2021   Practical Training  

  • Practice for Large Animal Clinics

    2021   Practical Training  

  • Field Practice for Large Animal Clinics

    2021   Practical Training  

  • Stock Farm Practice

    2021   Practical Training  

  • Large Animal Practice B

    2021    

  • Large Animal Practice A

    2021   Practical Training  

  • Veterinary Cardiovascular & Respiratory Medicine

    2021    

  • Theriogenology A

    2021    

  • Veterinary Clinical Nutrition

    2021    

  • Practice in Theriogenology

    2021   Practical Training  

  • Farm Animal Production Medicine

    2021    

  • Biology B

    2021    

  • Basic clinical practices

    2021   Practical Training  

  • Special Lecture: Advanced Pathobiology C

    2020    

  • Practical Training for Food  production

    2020   Practical Training  

  • Practice for Large Animal Clinics

    2020   Practical Training  

  • Field Practice for Large Animal Clinics

    2020   Practical Training  

  • Stock Farm Practice

    2020   Practical Training  

  • Practice in Veterinary English

    2020    

  • Large Animal Practice A

    2020   Practical Training  

  • Veterinary Cardiovascular & Respiratory Medicine

    2020    

  • Theriogenology A

    2020    

  • Veterinary Clinical Nutrition

    2020    

  • Fundamental Experiments of Biology

    2020   Practical Training  

  • Practice in Theriogenology

    2020   Practical Training  

  • Farm Animal Production Medicine

    2020    

  • Basic clinical practices

    2020   Practical Training  

  • 先端機器特別演習

    2020    

  • Large Animal Practice A

    2019   Practical Training  

  • Practical Training for Food  production

    2019   Practical Training  

  • Basic clinical practices

    2019   Practical Training  

  • Farm Animal Production Medicine

    2019    

  • Fundamental Experiments of Biology

    2019   Practical Training  

  • Practice for Large Animal Clinics

    2019   Practical Training  

  • Field Practice for Large Animal Clinics

    2019   Practical Training  

  • Stock Farm Practice

    2019   Practical Training  

  • Practice in Veterinary English

    2019    

  • Practice in Theriogenology

    2019   Practical Training  

  • Theriogenology A

    2019    

  • Veterinary Cardiovascular & Respiratory Medicine

    2019    

  • Veterinary Clinical Nutrition

    2019    

  • Special Lecture: Advanced Pathobiology C

    2019    

▼display all

Media Coverage

  • 「夢ナビ」において高校生向けの講義を公開 Internet

    2021

  • 「北海道新聞」に講演内容掲載 Newspaper, magazine

    北海道新聞  2018.11

  • 「開拓情報」に研究成果掲載 Newspaper, magazine

    開拓情報  2018.09