Updated on 2024/04/18

写真a

 
KASAMATSU Shingo
 
Organization
Graduate School of Science Department of Biological Chemistry Associate Professor
School of Science Department of Biological Chemistry
Title
Associate Professor
Affiliation
Institute of Science
Contact information
メールアドレス
Affiliation campus
Nakamozu Campus

Position

  • Graduate School of Science Department of Biological Chemistry 

    Associate Professor  2024.04 - Now

  • Graduate School of Science Department of Biological Chemistry 

    Assistant Professor  2022.04 - 2024.03

  • School of Science Department of Biological Chemistry 

    Associate Professor  2024.04 - Now

  • School of Science Department of Biological Chemistry 

    Assistant Professor  2022.04 - 2024.03

Degree

  • Ph.D. (Science) （ Osaka Prefecture University ）

Research Areas

  • Life Science / Molecular biology

  • Life Science / Cell biology

  • Life Science / Medical biochemistry

Research Interests

  • Supersulfides

  • Hibernation

  • Redox biology

  • Sulfur biology

  • Reactive Oxygen Species

  • Reactive Sulfur Species

  • Nitric Oxide

  • Redox signaling

  • Protein modification

Research subject summary

  • 生体内イオウ代謝系による低酸素・低体温耐性機構の解明

  • 活性イオウ分子種による一酸化窒素/活性酸素レドックスシグナル制御機構の解析

Professional Memberships

  • 日本NO学会

    2010.04 - Now   Domestic

  • 日本生化学会

    2010.09 - Now   Domestic

  • 日本酸化ストレス学会

    2023.04 - Now   Domestic

  • 日本栄養・食糧学会

    2022.04 - Now   Domestic

  • 日本農芸化学会

    2022.04 - Now   Domestic

Awards

  • アサヒグループ財団研究賞

    笠松真吾

    2024.04   公益財団法人アサヒグループ財団   超硫黄オミクス（Supedrsulfidomics）解析技術の開発

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    Country：Japan

  • Young Investigator Award

    2011.06  

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    Country：Japan

  • 第85回日本衛生学会学術集会　優秀演題賞

    2015.03   日本衛生学会  

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    Country：Japan

  • The Course for Business Planning in the Technology-based Entrepreneurship Course I 成績最優秀者表彰

    笠松真吾

    2012.03   大阪府立大学地域・産業牽引型高度人材育成プログラム運営員会   The Course for Business Planning in the Technology-based Entrepreneurship Course I 成績最優秀者表彰

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    Country：Japan

Job Career (off-campus)

  • Osaka Mettopolitan University   Department of Biological Chemistry, Graduate School of Science

    2024.04 - Now

  • Osaka Metropolitan University   Department of Biological Chemistry, Graduate School of Science   Assistant Professor

    2022.04 - Now

  • Osaka Prefecture University   Department of Biological Science, School of Science   Assistant Professor

    2019.04 - 2022.03

  • Depr. of Envir. Med. and Mol. Toxicol., Tohoku Univ. Grad. Sch. of Med   Lecturer

    2018.04 - Now

  • Harvard university Medical School, Massachusetts General Hospital   Research Fellow

    2017.04 - 2019.03

  • Depr. of Envir. Health Sci. and Mol. Toxicol., Tohoku Univ. Grad. Sch. of Med   Lecturer

    2017.04 - 2018.03

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Papers

  • Supersulfide catalysis for nitric oxide and aldehyde metabolism. Reviewed

    Shingo Kasamatsu, Akira Nishimura, Md Morshedul Alam, Masanobu Morita, Kakeru Shimoda, Tetsuro Matsunaga, Minkyung Jung, Seiryo Ogata, Uladzimir Barayeu, Tomoaki Ida, Motohiro Nishida, Akiyuki Nishimura, Hozumi Motohashi, Takaaki Akaike

    Science advances   9 ( 33 )   eadg8631   2023.08

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    Authorship：Lead author   Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Abundant formation of endogenous supersulfides, which include reactive persulfide species and sulfur catenated residues in thiols and proteins (supersulfidation), has been observed. We found here that supersulfides catalyze S-nitrosoglutathione (GSNO) metabolism via glutathione-dependent electron transfer from aldehydes by exploiting alcohol dehydrogenase 5 (ADH5). ADH5 is a highly conserved bifunctional enzyme serving as GSNO reductase (GSNOR) that down-regulates NO signaling and formaldehyde dehydrogenase (FDH) that detoxifies formaldehyde in the form of glutathione hemithioacetal. C174S mutation significantly reduced the supersulfidation of ADH5 and almost abolished GSNOR activity but spared FDH activity. Notably, Adh5C174S/C174S mice manifested improved cardiac functions possibly because of GSNOR elimination and consequent increased NO bioavailability. Therefore, we successfully separated dual functions (GSNOR and FDH) of ADH5 (mediated by the supersulfide catalysis) through the biochemical analysis for supersulfides in vitro and characterizing in vivo phenotypes of the GSNOR-deficient organisms that we established herein. Supersulfides in ADH5 thus constitute a substantial catalytic center for GSNO metabolism mediating electron transfer from aldehydes.

    DOI： 10.1126/sciadv.adg8631

    PubMed

  • Development of methods for quantitative determination of the total and reactive polysulfides: Reactive polysulfide profiling in vegetables Reviewed

    Shingo Kasamatsu, Ayaka Kinno, Jun-ichi Hishiyama, Takaaki Akaike, Hideshi Ihara

    Food Chemistry   413   135610   2023.02（ ISSN:03088146 ）

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    Authorship：Lead author   Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    DOI： 10.1016/j.foodchem.2023.135610

    PubMed

  • High-Precision Sulfur Metabolomics Innovated by a New Specific Probe for Trapping Reactive Sulfur Species. Reviewed

    Shingo Kasamatsu, Tomoaki Ida, Taisei Koga, Kosho Asada, Hozumi Motohashi, Hideshi Ihara, Takaaki Akaike

    Antioxidants & redox signaling   34 ( 18 )   1407 - 1419   2021.01（ ISSN:1523-0864 ）

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    Authorship：Lead author   Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    Aims:
    Persulfides and other reactive sulfur species are endogenously produced in large amounts in vivo and participate in multiple cellular functions underlying physiological and pathological conditions. In the current study, we aimed to develop an ideal alkylating agent for use in sulfur metabolomics, particularly targeting persulfides and other reactive sulfur species, with minimal artifactual decomposition.
    Results:
    We synthesized a tyrosine-based iodoacetamide derivative, N-iodoacetyl l-tyrosine methyl ester (TME-IAM), which reacts with the thiol residue of cysteine identically to that of β-(4-hydroxyphenyl)ethyl iodoacetamide (HPE-IAM), a commercially available reagent. Our previous study revealed that although various electrophilic alkylating agents readily decomposed polysulfides, HPE-IAM exceptionally stabilized the polysulfides by inhibiting their alkaline hydrolysis. The newly synthesized TME-IAM stabilizes oxidized glutathione tetrasulfide more efficiently than other alkylating agents, including HPE-IAM, iodoacetamide, and monobromobimane. In fact, our quantitative sulfur-related metabolome analysis showed that TME-IAM is a more efficient trapping agent for endogenous persulfides/polysulfides containing a larger number of sulfur atoms in mouse liver and brain tissues compared with HPE-IAM.
    Innovation and Conclusions:
    We developed a novel iodoacetamide derivative, which is the most ideal reagent developed to date for detecting endogenous persulfides/polysulfides formed in biological samples, such as cultured cells, tissues, and plasma. This new probe may be useful for investigating the unique chemical properties of reactive persulfides, thereby enabling identification of novel reactive sulfur metabolites that remain unidentified because of their instability, and thus can be applied in high-precision sulfur metabolomics in redox biology and medicine. We did not perform any clinical experiments in this study.

    DOI： 10.1089/ars.2020.8073

    PubMed

  • SOD1 is an essential H2S detoxifying enzyme Reviewed International coauthorship

    Christopher H. Switzer, Shingo Kasamatsu, Hideshi Ihara, Philip Eaton

    Proceedings of the National Academy of Sciences   120 ( 3 )   e220504412   2023.01

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    Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    DOI： 10.1073/pnas.2205044120

  • Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics. Reviewed International coauthorship

    Takaaki Akaike, Tomoaki Ida, Fan-Yan Wei, Motohiro Nishida, Yoshito Kumagai, Md. Morshedul Alam, Hideshi Ihara, Tomohiro Sawa, Tetsuro Matsunaga, Shingo Kasamatsu, Akiyuki Nishimura, Masanobu Morita, Kazuhito Tomizawa, Akira Nishimura, Satoshi Watanabe, Kenji Inaba, Hiroshi Shima, Nobuhiro Tanuma, Minkyung Jung, Shigemoto Fujii, Yasuo Watanabe, Masaki Ohmuraya, Péter Nagy, Martin Feelisch, Jon M. Fukuto, Hozumi Motohashi

    8   1177   2017.10

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    Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    DOI： 10.1038/s41467-017-01311-y

  • Manganese Porphyrin-Containing Polymeric Micelles: A Novel Approach for Intracellular Catalytic Formation of Per/Polysulfide Species from a Hydrogen Sulfide Donor

    Young K.

    Advanced Healthcare Materials   13 ( 4 )   e2302429   2024.02（ ISSN:21922640 ）

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  • Quantitative profiling of supersulfides naturally occurring in dietary meats and beans.

    Shingo Kasamatsu, Ayaka Kinno, Chiharu Miura, Jun-Ichi Hishiyama, Kensuke Fukui, Shoji Kure, Kazunobu Tsumura, Tomoaki Ida, Tetsuro Matsunaga, Takaaki Akaike, Hideshi Ihara

    Analytical biochemistry   685   115392 - 115392   2023.11（ ISSN:00032697 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Sulfur is essential in the inception of life and crucial for maintaining human health. This mineral is primarily supplied through the intake of proteins and is used for synthesizing various sulfur-containing biomolecules. Recent research has highlighted the biological significance of endogenous supersulfides, which include reactive persulfide species and sulfur catenated residues in thiol and proteins. Ingestion of exogenous sulfur compounds is essential for endogenous supersulfide production. However, the content and composition of supersulfides in foods remain unclear. This study investigated the supersulfide profiles of protein-rich foods, including edible animal meat and beans. Quantification of the supersulfide content revealed that natto, chicken liver, and bean sprouts contained abundant supersulfides. In general, the supersulfide content in beans and their derivatives was higher than that in animal meat. The highest proportion (2.15 %) was detected in natto, a traditional Japanese fermented soybean dish. These results suggest that the abundance of supersulfides, especially in foods like natto and bean sprouts, may contribute to their health-promoting properties. Our findings may have significant biological implications and warrant developing novel dietary intervention for the human health-promoting effects of dietary supersulfides abundantly present in protein-rich foods such as natto and bean sprouts.

    DOI： 10.1016/j.ab.2023.115392

    PubMed

  • Untargeted polysulfide omics analysis of alternations in polysulfide production during the germination of broccoli sprouts

    Shingo Kasamatsu, Takuma Owaki, Somei Komae, Ayaka Kinno, Tomoaki Ida, Takaaki Akaike, Hideshi Ihara

    Redox Biology   67   102875 - 102875   2023.09（ ISSN:2213-2317 ）

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.redox.2023.102875

    PubMed

  • 2-Oxo-imidazole dipeptides inhibit peroxynitrite-dependent tyrosine nitration Reviewed

    Kana Matsukura, Somei Komae, Shingo Kasamatsu, Hideshi Ihara

    Biochemical and Biophysical Research Communications   668   77 - 81   2023.08（ ISSN:0006-291X ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    DOI： 10.1016/j.bbrc.2023.05.074

    PubMed

  • Cystathionine γ-Lyase Self-Inactivates by Polysulfidation during Cystine Metabolism. Reviewed

    Shoma Araki, Tsuyoshi Takata, Katsuhiko Ono, Tomohiro Sawa, Shingo Kasamatsu, Hideshi Ihara, Yoshito Kumagai, Takaaki Akaike, Yasuo Watanabe, Yukihiro Tsuchiya

    International journal of molecular sciences   24 ( 12 )   2023.06（ ISSN:16616596 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Cystathionine γ-lyase (CSE) is an enzyme responsible for the biosynthesis of cysteine from cystathionine in the final step of the transsulfuration pathway. It also has β-lyase activity toward cystine, generating cysteine persulfide (Cys-SSH). The chemical reactivity of Cys-SSH is thought to be involved in the catalytic activity of particular proteins via protein polysulfidation, the formation of -S-(S)n-H on their reactive cysteine residues. The Cys136/171 residues of CSE have been proposed to be redox-sensitive residues. Herein, we investigated whether CSE polysulfidation occurs at Cys136/171 during cystine metabolism. Transfection of wild-type CSE into COS-7 cells resulted in increased intracellular Cys-SSH production, which was significantly increased when Cys136Val or Cys136/171Val CSE mutants were transfected, instead of the wild-type enzyme. A biotin-polyethylene glycol-conjugated maleimide capture assay revealed that CSE polysulfidation occurs at Cys136 during cystine metabolism. In vitro incubation of CSE with CSE-enzymatically synthesized Cys-SSH resulted in the inhibition of Cys-SSH production. In contrast, the mutant CSEs (Cys136Val and Cys136/171Val) proved resistant to inhibition. The Cys-SSH-producing CSE activity of Cys136/171Val CSE was higher than that of the wild-type enzyme. Meanwhile, the cysteine-producing CSE activity of this mutant was equivalent to that of the wild-type enzyme. It is assumed that Cys-SSH-producing CSE activity could be auto-inactivated via the polysulfidation of the enzyme during cystine metabolism. Thus, the polysulfidation of CSE at the Cys136 residue may be an integral feature of cystine metabolism, which functions to down-regulate Cys-SSH synthesis by the enzyme.

    DOI： 10.3390/ijms24129982

    PubMed

  • Reactive Sulfur Species Omics Analysis in the Brain Tissue of the 5xFAD Mouse Model of Alzheimer's Disease. Reviewed

    Ayaka Kinno, Shingo Kasamatsu, Takaaki Akaike, Hideshi Ihara

    Antioxidants (Basel, Switzerland)   12 ( 5 )   2023.05（ ISSN:2076-3921 ）

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    Publishing type：Research paper (scientific journal)   International / domestic magazine：International journal  

    Alzheimer's disease (AD) is a progressive neurodegenerative disorder whereby oxidative stress augmentation results in mitochondrial dysfunction and cell death by apoptosis. Emerging evidence indicates that reactive sulfur species (RSS), such as glutathione hydropersulfide (GSSH), is endogenously produced, functions as potent antioxidants, and regulate redox signaling through the formation of protein polysulfides. However, the relationship between RSS and AD pathogenesis is not fully understood. In this study, we analyzed endogenous RSS production in the brain tissue of a familial AD model (5xFAD) mouse using multiple RSS-omics approaches. Memory impairment, increased amyloid plaques, and neuroinflammation have been confirmed in 5xFAD mice. Quantitative RSS omics analysis revealed that the total polysulfide content was significantly decreased in the brains of 5xFAD mice, whereas there was no significant difference in the levels of glutathione, GSSH, or hydrogen sulfide between wild-type and 5xFAD mice. In contrast, a significant decline in the protein polysulfide status was observed in the brains of 5xFAD mice, suggesting that RSS production and subsequent redox signaling might be altered during the onset and progression of AD. Our findings have important implications for understanding the significance of RSS in the development of preventive and therapeutic strategies for AD.

    DOI： 10.3390/antiox12051105

    PubMed

  • Quantitative Determination of 2-Oxo-Imidazole-Containing Dipeptides by High-Performance Liquid Chromatography/Tandem Mass Spectrometry Reviewed

    Somei Komae, Shingo Kasamatsu, Koji Uchida, Hideshi Ihara

    Antioxidants (Basel, Switzerland)   11 ( 12 )   2401   2022.12（ ISSN:2076-3921 ）

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    Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    DOI： 10.3390/antiox11122401

    PubMed

  • The Sulfide-Responsive SqrR/BigR Homologous Regulator YgaV of Escherichia coli Controls Expression of Anaerobic Respiratory Genes and Antibiotic Tolerance Reviewed

    Rajalakshmi Balasubramanian, Koichi Hori, Takayuki Shimizu, Shingo Kasamatsu, Kae Okamura, Kan Tanaka, Hideshi Ihara, Shinji Masuda

    Antioxidants (Basel, Switzerland)   11 ( 12 )   2359   2022.11（ ISSN:2076-3921 ）

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    Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    DOI： 10.3390/antiox11122359

    PubMed

  • 2-Oxo-imidazole dipeptides in meat Reviewed

    Somei Komae, Shingo Kasamatsu, Hideshi Ihara

    Bioscience, biotechnology, and biochemistry   86 ( 11 )   1576 - 1580   2022.08（ ISSN:0916-8451 ）

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    Authorship：Lead author   Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    DOI： 10.1093/bbb/zbac138

    PubMed

  • Regulation of nitric oxide/reactive oxygen species redox signaling by nNOS splicing variants. Reviewed

    Shingo Kasamatsu, Hiroyasu Tsutsuki, Tomoaki Ida, Tomohiro Sawa, Yasuo Watanabe, Takaaki Akaike, Hideshi Ihara

    Nitric oxide : biology and chemistry   120   44 - 52   2022.01（ ISSN:1089-8603 ）

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    Authorship：Lead author   Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    We previously demonstrated different expression patterns of the neuronal nitric oxide synthase (nNOS) splicing variants, nNOS-μ and nNOS-α, in the rat brain; however, their exact functions have not been fully elucidated. In this study, we compared the enzymatic activities of nNOS-μ and nNOS-α and investigated intracellular redox signaling in nNOS-expressing PC12 cells, stimulated with a neurotoxicant, 1-methyl-4-phenylpyridinium ion (MPP+), to enhance the nNOS uncoupling reaction. Using in vitro studies, we show that nNOS-μ produced nitric oxide (NO), as did nNOS-α, in the presence of tetrahydrobiopterin (BH4), an important cofactor for the enzymatic activity. However, nNOS-μ generated more NO and less superoxide than nNOS-α in the absence of BH4. MPP + treatment induced more reactive oxygen species (ROS) production in nNOS-α-expressing PC12 cells than in those expressing nNOS-μ, which correlated with the intracellular production of 8-nitroguanosine 3',5'-cyclic monophosphate (8-nitro-cGMP), a downstream messenger of nNOS redox signaling, and apoptosis in these cells. Furthermore, post-treatment with 8-nitro-cGMP aggravated MPP+-induced cytotoxicity via activation of the H-Ras/extracellular signal-regulated kinase signaling pathway. In conclusion, our results provide strong evidence that nNOS-μ exhibits distinctive enzymatic properties of NO/ROS production, contributing to the regulation of intracellular redox signaling, including the downstream production of 8-nitro-cGMP.

    DOI： 10.1016/j.niox.2022.01.004

    PubMed

  • 2-Oxo-Imidazole-Containing Dipeptides Play a Key Role in the Antioxidant Capacity of Imidazole-Containing Dipeptides. Reviewed

    Shingo Kasamatsu, Somei Komae, Kana Matsukura, Yuki Kakihana, Koji Uchida, Hideshi Ihara

    Antioxidants (Basel, Switzerland)   10 ( 9 )   2021.09

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    Authorship：Lead author   Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    There is substantial evidence for the antioxidant functions of imidazole-containing dipeptides (IDPs), including carnosine and anserine, under physiological and pathological conditions in vivo. However, the detailed mechanism underlying the antioxidant functions is still poorly understood. Recently, we discovered the endogenous production of 2-oxo-imidazole-containing dipeptides (2-oxo-IDPs), such as 2-oxo-carnosine and 2-oxo-anserine, as novel derivatives of IDPs in mouse tissues and revealed that the antioxidant capacity of 2-oxo-carnosine was much greater than that of carnosine. However, the antioxidant capacity of 2-oxo-IDPs still remains unclear. In this study, we evaluated 2-oxo-carnosine and 2-oxo-anserine by multiple in vitro assays, such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing/antioxidant power, and oxygen radical absorbance capacity assays in comparison with the corresponding IDPs, carnosine and anserine. All the assays employed herein demonstrated that 2-oxo-carnosine and 2-oxo-anserine exhibited a greater antioxidant capacity than that of the corresponding IDPs. Quantitative high-performance liquid chromatography tandem mass spectrometry revealed that commercial IDPs standards were contaminated with a certain amount of 2-oxo-IDPs, which was correlated with the antioxidant capacity. DPPH radical scavenging assay revealed that the elimination of contaminated 2-oxo-IDPs from the IDPs standards caused a significant decrease in the antioxidant capacity compared to the original IDPs standards. These results suggest that the main driver of the antioxidant capacity of IDPs is 2-oxo-IDPs; accordingly, the conversion of IDPs to 2-oxo-IDPs may be a critical step in the antioxidant functions.

    DOI： 10.3390/antiox10091434

    PubMed

  • Distribution and quantitative analysis of homoanserine and its 2-oxo derivative in mouse tissues. Reviewed

    Yuki Kakihana, Shingo Kasamatsu, Koji Uchida, Hideshi Ihara

    Free radical research   55 ( 6 )   688 - 697   2021.07（ ISSN:1071-5762 ）

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    Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    Imidazole-containing dipeptides (IDPs), such as carnosine and anserine, are endogenously produced and have been shown to function as antioxidants. Recently, we have characterized the endogenous production of 2-oxo-imidazole-containing dipeptides (2-oxo-IDPs), such as 2-oxo-carnosine, 2-oxo-anserine, and 2-oxo-homocarnosine in mouse tissues, including brain, and demonstrated that 2-oxo-IDPs exhibit higher antioxidant activities than the corresponding IDPs. In this study, we established a highly sensitive, specific, and quantitative method for the detection of the IDP homoanserine and its oxidized derivative 2-oxo-homoanserine via high-performance liquid chromatography tandem mass spectrometry coupled with a stable-isotope dilution method, and quantitatively analyzed its tissue distribution and age-related intra-brain distribution in C57BL/6J mice. The quantitative analysis revealed that homoanserine exists abundantly not only in the mouse brain but also in other tissues, such as the muscle and lungs. Further, we successfully detected the endogenous production of 2-oxo-homoanserine in the mouse brain. The mass spectrometric analysis revealed that homoanserine predominantly exists in the cerebrum and cerebellum and the concentrations in 10-week-old mice were approximately 50-fold higher than those in 1-week-old mice. Accordingly, this is the first study that reports the spatial and temporal expression patterns of homoanserine and its 2-oxo derivative in C57BL/6J mice.

    DOI： 10.1080/10715762.2021.1888945

    PubMed

  • Regulation of redox signaling by reactive sulfur species. Reviewed

    Shingo Kasamatsu, Hideshi Ihara

    Journal of clinical biochemistry and nutrition   68 ( 2 )   111 - 115   2021.03（ ISSN:0912-0009 ）

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    Authorship：Lead author   Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    DOI： 10.3164/jcbn.20-124

    PubMed

  • Gene therapy for tuberous sclerosis complex type 2 in a mouse model by delivery of AAV9 encoding a condensed form of tuberin. Reviewed International coauthorship

    Pike-See Cheah, Shilpa Prabhakar, David Yellen, Roberta L Beauchamp, Xuan Zhang, Shingo Kasamatsu, Roderick T Bronson, Elizabeth A Thiele, David J Kwiatkowski, Anat Stemmer-Rachamimov, Bence György, King-Hwa Ling, Masao Kaneki, Bakhos A Tannous, Vijaya Ramesh, Casey A Maguire, Xandra O Breakefield

    Science advances   7 ( 2 )   eabb1703   2021.01

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    Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    Tuberous sclerosis complex (TSC) results from loss of a tumor suppressor gene - TSC1 or TSC2, encoding hamartin and tuberin, respectively. These proteins formed a complex to inhibit mTORC1-mediated cell growth and proliferation. Loss of either protein leads to overgrowth lesions in many vital organs. Gene therapy was evaluated in a mouse model of TSC2 using an adeno-associated virus (AAV) vector carrying the complementary for a "condensed" form of human tuberin (cTuberin). Functionality of cTuberin was verified in culture. A mouse model of TSC2 was generated by AAV-Cre recombinase disruption of Tsc2-floxed alleles at birth, leading to a shortened lifespan (mean 58 days) and brain pathology consistent with TSC. When these mice were injected intravenously on day 21 with AAV9-cTuberin, the mean survival was extended to 462 days with reduction in brain pathology. This demonstrates the potential of treating life-threatening TSC2 lesions with a single intravenous injection of AAV9-cTuberin.

    DOI： 10.1126/sciadv.abb1703

    PubMed

  • Myostatin deficiency not only prevents muscle wasting but also improves survival in septic mice. Reviewed International coauthorship

    Masayuki Kobayashi, Shingo Kasamatsu, Shohei Shinozaki, Shingo Yasuhara, Masao Kaneki

    American journal of physiology. Endocrinology and metabolism   320 ( 1 )   E150-E159 - E159   2021.01（ ISSN:0193-1849 ）

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    Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    Sepsis remains a leading cause of mortality in critically ill patients. Muscle wasting is a major complication of sepsis and negatively affects clinical outcomes. Despite intense investigation for many years, the molecular mechanisms underlying sepsis-related muscle wasting are not fully understood. In addition, a potential role of muscle wasting in disease development of sepsis has not been studied. Myostatin is a myokine that downregulates skeletal muscle mass. We studied the effects of myostatin deficiency on muscle wasting and other clinically relevant outcomes, including mortality and bacterial clearance, in mice. Myostatin deficiency prevented muscle atrophy along with inhibition of increases in muscle-specific RING finger protein 1 (MuRF-1) and atrogin-1 expression and phosphorylation of signal transducer and activator of transcription protein 3 (STAT3; major players of muscle wasting) in septic mice. Moreover, myostatin deficiency improved survival and bacterial clearance of septic mice. Sepsis-induced liver dysfunction, acute kidney injury, and neutrophil infiltration into the liver and kidney were consistently mitigated by myostatin deficiency, as indicated by plasma concentrations of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and neutrophil gelatinase-associated lipocalin (NGAL) and myeloperoxidase activity in the organs. Myostatin deficiency also inhibited sepsis-induced increases in plasma high-mobility group protein B1 (HMGB1) and macrophage inhibitory cytokine (MIC)-1/growth differentiation factor (GDF)-15 concentrations. These results indicate that myostatin plays an important role not only in muscle wasting but also in other clinically relevant outcomes in septic mice. Furthermore, our data raise the possibility that muscle wasting may not be simply a complication, but myostatin-mediated muscle cachexia and related changes in muscle may actually drive the development of sepsis as well.NEW & NOTEWORTHY Muscle wasting is a major complication of sepsis, but its role in the disease development is not known. Myostatin deficiency improved bacterial clearance and survival and mitigated damage in the liver and kidney in septic mice, which paralleled prevention of muscle wasting. These results raise the possibility that muscle wasting may not simply be a complication of sepsis, but myostatin-mediated cachexic changes may have a role in impaired bacterial clearance and mortality in septic mice.

    DOI： 10.1152/ajpendo.00161.2020

    PubMed

  • Persulfide-Dependent Regulation of Electrophilic Redox Signaling in Neural Cells. Reviewed

    Shingo Kasamatsu

    Antioxidants & redox signaling   33 ( 18 )   1320 - 1331   2020.12（ ISSN:1523-0864 ）

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    Authorship：Lead author,　Corresponding author   Publishing type：Research paper (scientific journal)   Kind of work：Single Work   International / domestic magazine：International journal  

    Significance: Redox homeostasis is precisely modulated by intricate systems that regulate production, elimination, and metabolism of electrophilic substances (electrophiles) in the nervous system. Since the first report of the endogenous production of reactive persulfide species in cells, such as cysteine persulfides (CysSSH), these reactive species have been a topic of extreme interest in the field of redox biology; persulfides/polysulfides possess unique chemical properties and are involved in multiple cellular functions. Recent Advances: Electrophilic signaling is mainly regulated by endogenous electrophiles that are generated from reactive oxygen species, nitric oxide, and their derivatives during stress responses, as well as by exogenous electrophiles, including compounds in foods and environmental pollutants, such as methylmercury (MeHg). Among diverse electrophiles that are endogenously generated, 8-nitroguanosine 3',5'-cyclic monophosphate (8-nitro-cGMP) possesses unique redox properties, of which the biosynthetic pathway, signaling mechanism, and metabolism in cells have been elucidated. Critical Issues: Persulfides, such as CysSSH, that are endogenously produced are critically involved in 8-nitro-cGMP metabolism. Exposure of neurons to the exogenous neurotoxicant, MeHg, causes severe neurodegeneration via disruption of persulfide-dependent 8-nitro-cGMP metabolism. Future Directions: Accumulating evidence indicates that persulfides are involved in various cellular functions under physiological and pathological conditions. These new aspects of redox biology related to persulfides may be frontiers of cell research, medical and clinical investigations of neurodegenerative diseases, as well as other fields. 8-Nitro-cGMP-mediated signaling and its persulfide-dependent metabolism in cells could, therefore, be potential targets for drug development, which may lead to the discovery of new therapeutic agents for many diseases, including neurodegenerative diseases.

    DOI： 10.1089/ars.2020.8130

    PubMed

  • Generation of Rat Monoclonal Antibody to Detect Hydrogen Sulfide and Polysulfides in Biological Samples. Reviewed

    Shingo Kasamatsu, Yuki Kakihana, Taisei Koga, Hisashi Yoshioka, Hideshi Ihara

    Antioxidants (Basel, Switzerland)   9 ( 11 )   1160   2020.11

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    Authorship：Lead author   Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    Hydrogen sulfide (H2S) is endogenously produced by enzymes and via reactive persulfide/polysulfide degradation; it participates in a variety of biological processes under physiological and pathological conditions. H2S levels in biological fluids, such as plasma and serum, are correlated with the severity of various diseases. Therefore, development of a simple and selective H2S measurement method would be advantageous. This study aimed to generate antibodies specifically recognizing H2S derivatives and develop a colorimetric immunoassay for measuring H2S in biological samples. We used N-ethylmaleimide (NEM) as an H2S detection agent that forms a stable bis-S-adduct (NEM-S-NEM). We also prepared bis-S-heteroadduct with 3-maleimidopropionic acid, which, in conjugation with bovine serum albumin, was to immunize Japanese white rabbits and Wistar rats to enable generation of polyclonal and monoclonal antibodies, respectively. The generated antibodies were evaluated by competitive enzyme-linked immunosorbent assay. We could obtain two stable hybridoma cell lines producing monoclonal antibodies specific for NEM-S-NEM. By immunoassay with the monoclonal antibody, the H2S level in mouse plasma was determined as 0.2 μM, which was identical to the level detected by mass spectrometry. Taken together, these monoclonal antibodies can be a useful tool for a simple and highly selective immunoassay to detect H2S in biological samples.

    DOI： 10.3390/antiox9111160

    PubMed

  • 8-Nitro-cGMP modulates exocytosis in adrenal chromaffin cells. Reviewed

    Hiroyasu Tsutsuki, Shingo Kasamatsu, Kohei Kunieda, Tomoaki Ida, Tomohiro Sawa, Nobuyuki Sasakawa, Takaaki Akaike, Hideshi Ihara

    Biochemical and biophysical research communications   526 ( 1 )   225 - 230   2020.03（ ISSN:0006-291X ）

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    Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    Nitric oxide (NO)-mediated production of cyclic guanosine 3',5'-monophosphate (cGMP) is a crucial signaling pathway that controls a wide array of neuronal functions, including exocytotic neurotransmitter release. A novel nitrated derivative of cGMP, 8-nitro-cGMP, not only activates cGMP-dependent protein kinase (PKG), but also has membrane permeability and redox activity to produce superoxide and S-guanylated protein. To date, no studies have addressed the effects of 8-nitro-cGMP on exocytotic kinetics. Here, we aimed to assess the 8-nitro-cGMP-mediated modulation of the depolarization-evoked catecholamine release from bovine chromaffin cells. 8-Nitro-cGMP was produced in bovine chromaffin cells dependent on NO donor. Amperometric analysis revealed that 8-nitro-cGMP modulated the kinetic parameters of secretory spikes from chromaffin cells, particularly decreased the speed of individual spikes, resulting in a reduced amperometric spike height, slope β, and absolute value of slope γ. The modulatory effects were independent of the PKG signal and superoxide production. This is the first study to demonstrate that 8-nitro-cGMP modulates exocytosis and provide insights into a novel regulatory mechanism of exocytosis.

    DOI： 10.1016/j.bbrc.2020.03.045

    PubMed

  • 8-Nitro-cGMP attenuates context-dependent fear memory in mice Reviewed

    Yusuke Kishimoto, Shingo Kasamatsu, Shuichi Yanai, Shogo Endo, Takaaki Akaike, Hideshi Ihara

    Biochemical and Biophysical Research Communications   511 ( 1 )   141 - 147   2019.03（ ISSN:0006-291X ）

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    Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    © 2019 Elsevier Inc. We previously reported that 8-nitroguanosine 3′,5′-cyclic monophosphate (8-nitro-cGMP) is endogenously produced via nitric oxide/reactive oxygen species signaling pathways and it reacts with protein thiol residues to add cGMP structure to proteins through S-guanylation. S-Guanylation occurs on synaptosomal-associated protein 25 (SNAP-25), which is a part of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex that regulates exocytosis. However, the biological relevance of 8-nitro-cGMP in the nervous system remains unclear. Here, we investigated the effects of intracerebroventricular (icv) infusion of 8-nitro-cGMP on mouse brain functions. The results of an open-field test and fear-conditioning task revealed that icv infusion of 8-nitro-cGMP decreased the vertical activity and context-dependent fear memory of mice, which are both associated with the hippocampus. Immunohistochemical analysis revealed increased c-Fos-positive cells in the dentate gyrus in 8-nitro-cGMP-infused mice. Further, biochemical analyses showed that icv infusion of 8-nitro-cGMP increased S-guanylated proteins including SNAP-25 and SNARE complex formation as well as decreased complexes containing complexin, which regulates exocytosis by binding to the SNARE complex, in the hippocampus. These findings suggest that accumulation of 8-nitro-cGMP in the hippocampus affects its functions, including memory, via S-guanylation of hippocampal proteins such as SNAP-25.

    DOI： 10.1016/j.bbrc.2019.01.138

    PubMed

  • Breathing hydrogen sulfide prevents delayed paraplegia in mice. Reviewed International coauthorship

    Manabu Kakinohana, Eizo Marutani, Kentaro Tokuda, Kotaro Kida, Shizuko Kosugi, Shingo Kasamatsu, Aurora Magliocca, Kohei Ikeda, Shinichi Kai, Masahiro Sakaguchi, Shuichi Hirai, Ming Xian, Masao Kaneki, Fumito Ichinose

    Free radical biology & medicine   131   243 - 250   2019.02（ ISSN:0891-5849 ）

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    Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    Delayed paraplegia complicates the recovery from spinal cord ischemia or traumatic spinal cord injury. While delayed motor neuron apoptosis is implicated in the pathogenesis, no effective treatment or preventive measures is available for delayed paraplegia. Hydrogen sulfide exerts anti-apoptotic effects. Here, we examined effects of hydrogen sulfide breathing on the recovery from transient spinal cord ischemia. Breathing hydrogen sulfide starting 23 h after reperfusion for 5 h prevented delayed paraplegia after 5 min of spinal cord ischemia. Beneficial effects of hydrogen sulfide were mediated by upregulation of anti-apoptotic Bcl-XL and abolished by nitric oxide synthase 2 deficiency. S-nitrosylation of NFkB p65 subunit, which is induced by nitric oxide derived from nitric oxide synthase 2, facilitated subsequent sulfide-induced persulfidation of p65 and transcription of anti-apoptotic genes. These results uncover the molecular mechanism of the anti-apoptotic effects of sulfide based on the interaction between nitric oxide and sulfide. Exploitation of the anti-apoptotic effects of delayed hydrogen sulfide breathing may provide a new therapeutic approach for delayed paraplegia.

    DOI： 10.1016/j.freeradbiomed.2018.12.003

    PubMed

  • Involvement of nitric oxide/reactive oxygen species signaling via 8-nitro-cGMP formation in 1-methyl-4-phenylpyridinium ion-induced neurotoxicity in PC12 cells and rat cerebellar granule neurons. Reviewed

    Kumiko Masuda, Hiroyasu Tsutsuki, Shingo Kasamatsu, Tomoaki Ida, Tsuyoshi Takata, Kikuya Sugiura, Motohiro Nishida, Yasuo Watanabe, Tomohiro Sawa, Takaaki Akaike, Hideshi Ihara

    495 ( 3 )   2165 - 2170   2018.01

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    Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    DOI： 10.1016/j.bbrc.2017.12.088

  • Exposure to electrophiles impairs reactive persulfide-dependent redox signaling in neuronal cells. Reviewed

    Hideshi Ihara, Shingo Kasamatsu, Atsushi Kitamura, Akira Nishimura, Hiroyasu Tsutsuki, Tomoaki Ida, Kento Ishizaki, Takashi Toyama, Eiko Yoshida, Hisyam Abdul Hamid, Minkyung Jung, Tetsuro Matsunaga, Shigemoto Fujii, Tomohiro Sawa, Motohiro Nishida, Yoshito Kumagai, Takaaki Akaike

    Chemical Research in Toxicology   30   1673 - 1684   2017.08

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    Authorship：Lead author   Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    DOI： 10.1021/acs.chemrestox.7b00120

  • Superoxide generation from nNOS splice variants and its potential involvement in redox signal regulation. Reviewed

    Hideshi Ihara, Atsushi Kitamura, Shingo Kasamatsu, Tomoaki Ida, Yuki Kakihana, Hiroyasu Tsutsuki, Tomohiro Sawa, Yasuo Watanabe, Takaaki Akaike

    Biochemical Journal   474 ( 7 )   1149 - 1162   2017.01

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    Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    DOI： 10.1042/BCJ20160999

  • Redox Signaling Regulated by Cysteine Persulfide and Protein Polysulfidation Reviewed

    Shingo Kasamatsu, Akira Nishimura, Masanobu Morita, Tetsuro Matsunaga, Hisyam Abdul Hamid, Takaaki Akaike

    21 ( 12 )   1721   2016.12

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    Authorship：Lead author   Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    DOI： 10.3390/molecules21121721

  • Protein polysulfidation-dependent persulfide dioxygenase activity of ethylmalonic encephalopathy protein 1. Reviewed

    Minkyung Jung, Shingo Kasamatsu, Tetsuro Matsunaga, Soichiro Akashi, Katsuhiko Ono, Akira Nishimura, Masanobu Morita, Hisyam Abdul Hamid, Shigemoto Fujii, Hiroshi Kitamura, Tomohiro Sawa, Tomoaki Ida, Hozumi Motohashi, Takaaki Akaike

    Biochemical and Biophysical Research Communications   480 ( 2 )   180 - 186   2016.11

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    Authorship：Lead author   Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    DOI： 10.1016/j.bbrc.2016.10.022

  • 8-Nitro-cGMP enhances SNARE complex formation through S-guanylation of Cys90 in SNAP25. Reviewed

    Kohei Kunieda, Hiroyasu Tsutsuki, Tomoaki Ida, Yusuke Kishimoto, Shingo Kasamatsu, Tomohiro Sawa, Naoki Goshima, Makoto Itakura, Masami Takahashi, Takaaki Akaike, Hideshi Ihara

    ACS Chemical Neuroscience   6 ( 10 )   1715 - 1725   2015.10

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    Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    DOI： 10.1021/acschemneuro.5b00196

  • Redox signal regulation via nNOS phosphorylation at Ser847 in PC12 cells and rat cerebellar granule neurons. Reviewed

    Shingo Kasamatsu, Yasuo Watanabe, Tomohiro Sawa, Takaaki Akaike, Hideshi Ihara

    459 ( 2 )   251 - 563   2014.04

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    Authorship：Lead author   Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    DOI： 10.1042/bj20131262

  • Regulation by mitochondrial superoxide and NADPH oxidase of cellular formation of nitrated cyclic GMP: potential implications for ROS signalling. Reviewed

    Khandaker Ahtesham Ahmed, Tomohiro Sawa, Hideshi Ihara, Shingo Kasamatsu, Jun Yoshitake, Md Mizanur Rahaman, Tatsuya Okamoto, Shigemoto Fujii, Takaaki Akaike

    441 ( 2 )   719 - 730   2012.01

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    Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    DOI： 10.1042/BJ20111130

  • Methodological proof of immunochemistry for specific identification of 8-nitroguanosine 3',5'-cyclic monophosphate formed in glia cells. Reviewed

    Hideshi Ihara, Ahmed Khandaker Ahtesham, Tomoaki Ida, Shingo Kasamatsu, Kouhei Kunieda, Tatsuya Okamoto, Tomohiro Sawa, Takaaki Akaike

    25 ( 2 )   169 - 175   2011.08

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    Publishing type：Research paper (scientific journal)   Kind of work：Joint Work   International / domestic magazine：International journal  

    DOI： 10.1016/j.niox.2011.04.015

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MISC

  • Development of Novel Method for Analysis of Supersulfides

    Shingo Kasamatsu and Hideshi Ihara

    93 ( 5 )   613 - 620   2021.10

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  • 質量分析法を用いた生体内活性硫黄分子の網羅的解析

    笠松真吾, 居原秀

    実験医学   39 ( 13 )   2106 - 2108   2021.08

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    Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)   Kind of work：Joint Work  

  • 活性イオウ分子を基軸とした大豆たん白質および関連成分の基礎的研究—Fundamental Research on Soy Protein and Related Components Based on Reactive Sulfur Species—第24回研究報告会記録

    居原 秀, 赤池 孝章, 中島 秀満, 笠松 真吾

    大豆たん白質研究 / 不二たん白質研究振興財団 [編]   24 ( 42 )   1 - 5   2022.07（ ISSN:1344-4050 ）

  • 食品の超硫黄による環境化学物質の毒性制御 Invited

    笠松 真吾, 居原 秀

    Medical Science Digest   49 ( 1 )   25 - 28   2022.01（ ISSN:1347-4340 ）

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    Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)  

    食品中の硫黄成分の健康増強効果は古代エジプトの時代から認識されてきたが,その詳細な分子メカニズムは不明な点が多い。近年,超硫黄分子が生体内に豊富に存在し,様々な細胞機能調節に寄与していることがわかってきた。本項では,生体内および食品中に存在する超硫黄分子について概説するとともに,超硫黄分子による環境化学物質の毒性制御機構に関する最新の知見を紹介する。(著者抄録)

  • 超硫黄分子の新規分析方法の開発 Invited

    笠松 真吾, 居原 秀

    生化学   93 ( 5 )   613 - 620   2021.10（ ISSN:0037-1017 ）

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    Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

    生体内レドックスホメオスタシスは,親電子性物質の産生・除去・代謝を介して,精密に制御されている.システインパースルフィド(CysSSH)などの超硫黄分子は,種横断的に生体内で内因的に産生され,強力な抗酸化活性・レドックスシグナル制御活性を示す生理活性物質である.近年,超硫黄分子のユニークな化学特性が徐々に明らかにされるとともに,さまざまな新規分析技術が開発され,タンパク質翻訳時にCysSSHが新生鎖ペプチドに取り込まれることや,哺乳動物のミトコンドリアにおいてCysSSHとその関連硫黄代謝物がエネルギー生成およびミトコンドリア生合成に利用されていることなど,多彩な細胞機能に関与することが報告されてきている.さらに,疾患との関連や,食品中の超硫黄分子の存在様式についての知見が集まりつつある.(著者抄録)

  • 質量分析法を用いた生体内活性硫黄分子の網羅的解析

    笠松 真吾, 居原 秀

    実験医学   39 ( 13 )   2106 - 2108   2021.08（ ISSN:0288-5514 ）

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    Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)  

  • Development of a novel method for analyzing supersulfides

    笠松真吾, 居原秀

    生化学   93 ( 5 )   2021（ ISSN:0037-1017 ）

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    Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

    J-GLOBAL

  • 質量分析法を用いた生体内活性硫黄分子の網羅的解析

    笠松真吾, 居原秀

    実験医学   39 ( 13 )   2021（ ISSN:0288-5514 ）

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    Authorship：Lead author   Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)  

    J-GLOBAL

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Presentations

  • A mass spectrometry-based technology for exploration of endogenous persulfides/polysulfides Invited International conference

    Shingo kasamatsu, Hideshi Ihara

    The 12th International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide  2022.10  Nitric Oxide Society

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    Presentation type：Symposium, workshop panel (nominated)  

  • nNOS splice variants regulate NO/ROS redox signaling via 8-nitro-cGMP formation: its potential involvement in Parkinson's disease-like neurotoxicity International conference

    Shingo Kasamatsu, Kumiko Masuda, Hiroyasu Tsutsuki, Tomoaki Ida, Tsuyoshi Takata, Motohiro Nishida, Yasuo Watanabe, Tomohiro Sawa, Takaaki Akaike, Hideshi Ihara

    Gordon Research Conference - Nitric Oxide　2023  2023.02  Gordon Research Conferences

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    Presentation type：Poster presentation  

  • Supersulfides Catalyze Nitric Oxide Metabolism via Glutathione-coupled Electron Transfer from Formaldehyde International conference

    Masanobu Morita, Tetsuro Matsunaga, Shingo Kasamatsu, Akira Nishimura, Md. Morshedul Alam, Kakeru Shimoda, Akiyuki Nishimura, Seiryo Ogata, Minkyung Jung, Tomoaki Ida,Motohiro Nishida, Hozumi Motohashi, Takaaki Akaike

    Gordon Research Conference - Nitric Oxide　2023  2023.02  Gordon Research Conferences

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    Presentation type：Poster presentation  

  • High precision sulfur metabolomics innovated by a new specific probe for trapping reactive sulfur species Invited International conference

    Shingo Kasamatsu, Tomoaki Ida, Kosho Asada, Taisei Koga, Hozumi Motohashi, Takaaki Akaike, Hideshi Ihara

    6th World congress on hydrogen sulfide in biology & medicine  2022.05 

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    Presentation type：Oral presentation (general)  

  • Disruption of reactive persulfide-dependent redox signal by exposure of electrophile in neuronal cells Invited International conference

    Shingo Kasamatsu and Hideshi Ihara

    1st International Conference on Persulfides and Sulfur Metabolism in Biology and Medicine  2019.09  Persulfide conference

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    Presentation type：Symposium, workshop panel (nominated)  

  • 超硫黄触媒によるホルムアルデヒド代謝とNOシグナル制御

    守田 匡伸, 松永 哲郎, 笠松 真吾, Alam Md .Morshedul, Jung Minkyung, 緒方 星陵, Barayeu Uladzimir, 井田 智章, 西田 基宏, 本橋 ほづみ, 赤池 孝章

    日本生化学会大会プログラム・講演要旨集  2023.10  (公社)日本生化学会

  • 低温曝露による冬眠性・非冬眠性動物細胞内超硫黄分子生成動態への影響の解析

    笠松 真吾, 遠田 隆人, 赤池 孝章, 居原 秀

    日本生化学会大会プログラム・講演要旨集  2023.10  (公社)日本生化学会

  • アルツハイマー病モデルマウスの脳組織における超硫黄オミクス解析

    金野 文香, 笠松 真吾, 赤池 孝明, 居原 秀

    日本生化学会大会プログラム・講演要旨集  2023.10  (公社)日本生化学会

  • 食品中のポリスルフィドの解析 Domestic conference

    笠松真吾、金野文香、菱山純一、赤池孝章、居原秀

    日本農芸化学会　2023年度大会  2023.03  日本農芸化学会

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    Presentation type：Oral presentation (general)  

  • アルツハイマー病モデルマウスへのシラス酵素処理物投 与試験によるアミロイド β 蓄積抑制効果 Domestic conference

    隅谷栄伸、大木伽耶子、甲木孝弘、笠松真吾、 金野文香、居原秀

    日本農芸化学会　2023年度大会  2023.03  日本農芸化学会

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    Presentation type：Oral presentation (general)  

  • ブロッコリーの発芽段階における活性硫黄の解析 Domestic conference

    大脇拓真、笠松真吾、居原秀

    日本農芸化学会　2023年度大会  2023.03  日本農芸化学会

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    Presentation type：Oral presentation (general)  

  • 2-オキソイミダゾールジペプチドはペルオキシナイトライト依存的なチロシンのニトロ化を阻害する Domestic conference

    松倉加奈、小前奏明、笠松真吾、居原秀

    日本農芸化学会　2023年度大会  2023.03  日本農芸化学会

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    Presentation type：Oral presentation (general)  

  • 2-オキソイミダゾールジペプチドの高感度絶対定量法の確立 Domestic conference

    小前奏明、笠松真吾、内田浩二、居原秀

    日本農芸化学会　2023年度大会  2023.03  日本農芸化学会

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    Presentation type：Oral presentation (general)  

  • 活性硫黄分子保護効果を有する大豆成分の解析 Domestic conference

    居原秀、小前奏明、笠松真吾

    日本農芸化学会　2023年度大会  2023.03  日本農芸化学会

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    Presentation type：Oral presentation (general)  

  • 2-オキソイミダゾールジペプチドによる抗ニトロ化活性

    松倉 加奈, 笠松 真吾, 小前 奏明, 居原 秀

    日本栄養・食糧学会大会講演要旨集  2023.03  (公社)日本栄養・食糧学会

  • アルツハイマー病モデルマウスへのシラス由来成分投与によるアミロイドβ蓄積抑制効果

    甲木 孝弘, 隅谷 栄伸, 笠松 真吾, 金野 文香, 居原 秀

    日本栄養・食糧学会大会講演要旨集  2023.03  (公社)日本栄養・食糧学会

  • 大豆の発芽・成長過程におけるポリスルフィド生成動態変化の解析

    久龍 茉尋, 笠松 真吾, 居原 秀

    日本栄養・食糧学会大会講演要旨集  2023.03  (公社)日本栄養・食糧学会

  • 新規抗酸化活性物質2-オキソイミダゾールジペプチドの網羅的高感度定量法の確立

    小前 奏明, 笠松 真吾, 内田 浩二, 居原 秀

    日本栄養・食糧学会大会講演要旨集  2023.03  (公社)日本栄養・食糧学会

  • 網羅的ポリスルフィドオミクスによるブロッコリースプラウトの発芽・成長過程におけるポリスルフィド生成動態の解析

    笠松 真吾, 大脇 拓真, 小前 奏明, 金野 文香, 赤池 孝章, 居原 秀

    日本栄養・食糧学会大会講演要旨集  2023.03  (公社)日本栄養・食糧学会

  • 食品中の反応性ポリスルフィドプロファイル解析

    金野 文香, 笠松 真吾, 菱山 純一, 赤池 孝章, 居原 秀

    日本栄養・食糧学会大会講演要旨集  2023.03  (公社)日本栄養・食糧学会

  • 超硫黄触媒酵素: アルコールデヒドロゲナーゼ5（ADH5）によるNOシグナル機能の制御メカニズム Domestic conference

    守⽥匡伸、松永哲郎、笠松真吾、⻄村明、Md. Morshedul Alam、下⽥翔、⻄村明幸、緒⽅星陵、Jung Minkyung、井⽥智章、⻄⽥基宏、本橋ほづみ、⾚池孝章

    第22回分子予防環境医学研究会  2023.01  分子予防環境医学研究会

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  • S-Nitrosyl L-cysteine acts as suicidal substrate of cystathionine gamma-lyase International conference

    Shoma Araki, Yukihiro Tsuchiya, Shingo Kasamatsu, Hideshi Ihara, Hidehiko Nakagawa, Takaaki Akaike, Yasuo Watanabe

    The 12th International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide  2022.10  Nitric Oxide Society

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    Presentation type：Symposium, workshop panel (nominated)  

  • Omics analysis of reactive sulfur species in the brain tissue of mouse model of Alzheimer’s disease International conference

    Ayaka Kinno, Shingo Kasamastu, Takaaki Akaike, Hideshi Ihara

    The 12th International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide  2022.10  Nitric Oxide Society

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  • Analysis of the effects of germination on endogenous polysulfide production in soybean International conference

    Mahiro Kuryu, Shingo Kasamatsu, Hideshi Ihara

    The 12th International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide  2022.10  Nitric Oxide Society

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  • Analysis of polysulfide profile changes in broccoli during germination International conference

    Takuma Owaki , Shingo Kasamatsu , Hideshi Ihara

    The 12th International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide  2022.10  Nitric Oxide Society

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    Presentation type：Poster presentation  

  • Analysis of the inhibitory activity of 2-oxo-imidazole containing dipeptides against peroxynitrite-dependent nitration International conference

    Kana Matsukura, Shingo Kasamatsu, Somei Komae, Yuki Kakihana, Koji Uchida, Hideshi Ihara

    The 12th International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide  2022.10  Nitric Oxide Society

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  • Analysis of changes in sulfur metabolism in the brown adipose tissue of hibernation-induced golden hamsters International conference

    Airi Nishida, Shingo Kasamatsu, Takaaki Akaike, Hideshi Ihara

    The 12th International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide  2022.10  Nitric Oxide Society

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  • Investigation of the antioxidant mechanism of 2-oxo-imidazole containing dipeptides International conference

    Somei Komae, Shingo Kasamatsu, Kana Matsukura, Yuki Kakihana, Koji Uchida, Hideshi Ihara

    The 12th International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide  2022.10  Nitric Oxide Society

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  • Development of ionization-coupled cleavage of oxidized polysulfide structure (iCOPS): a novel detection method for oxidized polysulfide modifications on proteins International conference

    Kae Okamura, Shingo Kasamatsu, Takayuki Shimizu, Shinji Masuda, Hideshi Ihara

    The 12th International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide  2022.10  Nitric Oxide Society

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  • 新規の機能性酸化代謝産物 2-オキソイミダゾールジペプチドの抗酸化メカニズムに関する研究 Domestic conference

    小前 奏明、笠松 真吾、内田 浩二、居原 秀

    日本農芸化学会関西支部　第521回講演会  2022.07  日本農芸化学会関西支部

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    Presentation type：Oral presentation (general)  

  • 2-オキソイミダゾールジペプチドの定量法の確立と生体内産生 Domestic conference

    小前 奏明、笠松 真吾、内田 浩二、居原 秀

    第12回 カルノシン・アンセリン研究会  2022.06  カルノシン・アンセリン研究会

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  • Cysteine-based redox switches in cystathionine γ-lyase Invited International conference

    Yasuo Watanabe, Shoma Araki, Tsuyoshi Takata, Shingo Kasamatsu, Hideshi Ihara, Yukihiro Tsuchiya

    6th World congress on hydrogen sulfide in biology & medicine  2022.05 

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  • 超硫黄プロテオーム・メタボローム解析の注意点　超硫黄検出ことはじめ Invited Domestic conference

    笠松真吾

    第二回レドックスR&D戦略委員会　春のシンポジウム  2022.03  レドックスR&D戦略委員会

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    Presentation type：Public lecture, seminar, tutorial, course, or other speech  

  • アルツハイマー病モデルマウス脳内における超硫黄分子生成動態の解析 Domestic conference

    金野文香、笠松真吾、赤池孝章、居原秀

    第21回分子予防環境医学研究会  2022.02  分子予防環境医学研究会

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  • 2-オキソイミダゾールジペプチドの抗酸化メカニズムに関する研究 Domestic conference

    小前奏明、笠松真吾、内田浩二、居原秀

    第21回分子予防環境医学研究会  2022.02  分子予防環境医学研究会

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  • 超硫黄化タンパク質の特異的検出法　新規超硫黄ビオチンスイッチ法 Domestic conference

    Jung Minkyung, 笠松 真吾, 井田 智章, 松永 哲郎, 守田 匡伸, 本橋 ほづみ, 赤池 孝章

    第94回日本生化学会大会  2021.11  (公社)日本生化学会

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  • 新規アルキル化試薬N-iodoacetyl tyrosine methyl esterを用いた超硫黄メタボローム・プロテオーム解析系の構築 Domestic conference

    笠松 真吾, 井田 智章, 古賀 大聖, 浅田 康勝, 本橋 ほづみ, 赤池 孝章, 居原 秀

    第94回日本生化学会大会  2021.11  (公社)日本生化学会

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  • アルツハイマー病モデルマウス脳内における活性硫黄分子オミクス解析 Domestic conference

    金野 文香, 笠松 真吾, 赤池 孝章, 居原 秀

    第94回日本生化学会大会  2021.11  (公社)日本生化学会

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  • 2-オキソカルノシンの抗酸化メカニズムに関する研究 Domestic conference

    小前 奏明, 笠松 真吾, 内田 浩二, 居原 秀

    第94回日本生化学会大会  2021.11  (公社)日本生化学会

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  • 2-オキソカルノシンの抗酸化メカニズムに関する研究

    小前 奏明, 笠松 真吾, 内田 浩二, 居原 秀

    日本生化学会大会プログラム・講演要旨集  2021.11  (公社)日本生化学会

  • 新規アルキル化試薬N-iodoacetyl L-tyrosine methyl esterを用いた超硫黄メタボローム・プロテオーム解析系の構築 Domestic conference

    笠松真吾，井田智章，古賀大聖，浅田康勝，本橋ほづみ，赤池孝章2，居原秀

    生理研研究会2021 生命を支える硫黄生物学の最前線  2021.07  生理学研究所研究会

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  • 超硫黄化タンパク質の特異的検出法：新規超硫黄ビオチンスイッチ法 Domestic conference

    Jung Minkyung、笠松真吾、井田智章、松永哲郎、守田匡伸、本橋ほづみ、赤池孝章

    生理研研究会2021 生命を支える硫黄生物学の最前線  2021.07  生理学研究所研究会

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  • 2-オキソ-カルノシンはカルノシンの抗酸化活性の本体か? Domestic conference

    居原 秀, 笠松 真吾, 垣花 優希, 小前 奏明, 松倉 加奈, 内田 浩二

    第74回日本栄養・食糧学会大会  2021.07  (公社)日本栄養・食糧学会

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  • 2-オキソカルノシンの活性測定 Domestic conference

    松倉 加奈, 笠松 真吾, 小前 奏明, 内田 浩二, 居原 秀

    第74回日本栄養・食糧学会大会  2021.07  (公社)日本栄養・食糧学会

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  • 2-オキソイミダゾールジペプチドの抗酸化活性測定 Domestic conference

    小前 奏明, 笠松 真吾, 松倉 加奈, 内田 浩二, 居原 秀

    第74回日本栄養・食糧学会大会  2021.07  (公社)日本栄養・食糧学会

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  • 2-オキソカルノシンの活性測定

    松倉 加奈, 笠松 真吾, 小前 奏明, 内田 浩二, 居原 秀

    日本栄養・食糧学会大会講演要旨集  2021.07  (公社)日本栄養・食糧学会

  • 2-オキソ-カルノシンはカルノシンの抗酸化活性の本体か?

    居原 秀, 笠松 真吾, 垣花 優希, 小前 奏明, 松倉 加奈, 内田 浩二

    日本栄養・食糧学会大会講演要旨集  2021.07  (公社)日本栄養・食糧学会

  • 2-オキソイミダゾールジペプチドの抗酸化活性測定

    小前 奏明, 笠松 真吾, 松倉 加奈, 内田 浩二, 居原 秀

    日本栄養・食糧学会大会講演要旨集  2021.07  (公社)日本栄養・食糧学会

  • アルコールデヒドロゲナーゼ5（ADH5）のニトロソグルタチオン還元酵素（GSNOR）反応の選択的欠損マウスの開発 Domestic conference

    松永哲郎、笠松真吾、西村明、井田智章、守田匡伸、居原 秀、下田翔、西田基宏、本橋ほづみ、赤池孝章

    第74回 日本酸化ストレス学会・第21回 日本NO学会合同学術集会  2021.05  日本NO学会

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  • アルツハイマー病と活性硫黄代謝系の連関の解析 Domestic conference

    坂本拓斗，笠松真吾，居原　秀

    第20回日本NO学会/第73回日本酸化ストレス学会合同学会  2020.10  日本NO学会

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  • 新規アルキル化剤を用いた活性イオウ分子種の網羅的検出 Domestic conference

    浅田康勝, 笠松真吾, 古賀大聖, 坂本拓斗, 井田智章, 赤池孝章, 居原秀

    第20回日本NO学会/第73回日本酸化ストレス学会合同学会  2020.10  日本NO学会

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  • 活性イオウ分子種特異的新規アルキル化試薬の合成 Domestic conference

    笠松真吾、井田智章、浅田康勝 、古賀大聖、坂本拓斗、赤池孝章、居原　秀

    第20回日本NO学会/第73回日本酸化ストレス学会合同学会  2020.10  日本NO学会

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  • タンパク質活性硫黄の選択的検出系の開発 Domestic conference

    古賀大聖、笠松真吾、若森久幸、川口充康、中川秀彦、居原　秀

    第20回日本NO学会/第73回日本酸化ストレス学会合同学会  2020.10  日本NO学会

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  • 2-オキソ-カルノシンの抗炎症効果 Domestic conference

    垣花優希、笠松真吾、内田浩二、居原　秀

    第20回日本NO学会/第73回日本酸化ストレス学会合同学会  2020.10  日本NO学会

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  • 酸化イミダゾールジペプチドによる細胞機能調節 Domestic conference

    垣花 優希, 笠松 真吾, 内田 浩二, 居原 秀

    第73回日本栄養・食糧学会大会  2020.04  (公社)日本栄養・食糧学会

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  • 新規の抗酸化物質2-オキソ-カルノシンによる細胞機能調節 Domestic conference

    垣花優希、笠松真吾、内田浩二、居原 秀

    第19回分子予防環境医学研究会大会  2020.03  分子予防環境医学研究会

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  • 食品中の活性硫黄の定量解析 Domestic conference

    菱山純一、居原秀、笠松真吾

    日本農芸化学会2020年度大会  2020.03  日本農芸化学会

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  • 培地中の硫酸塩がユーグレナの活性イオウ分子の生成動態に及ぼす影響 Domestic conference

    神田彩華、笠松真吾、中野長久、居原 秀

    日本農芸化学会2020年度大会  2020.03  日本農芸化学会

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  • ホモアンセリンおよび酸化ホモアンセリンの測定方法の確立と生体内局在の解明 Domestic conference

    垣花優希、笠松真吾、内田浩二、居原 秀

    日本農芸化学会2020年度大会  2020.03  日本農芸化学会

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  • 1-Methyl-4-phenylpyridinium ion神経毒性における一酸化窒素/活性酸素種レドックスシグナル経路の関与 Domestic conference

    笠松真吾、西田基宏、澤智裕、熊谷嘉人、赤池孝章、居原秀

    第19回分子予防環境医学研究会大会  2020.03  分子予防環境医学研究会

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  • 新規アルキル化剤を用いたイオウメタボローム・プロテオーム解析系の構築 Invited Domestic conference

    笠松真吾

    レドックス・ライフイノベーション第170委員会 20周年記念 若手シンポジウム  2020.01  レドックス・ライフイノベーション第170委員会

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    Presentation type：Oral presentation (invited, special)  

  • イオウメタボローム・プロテオーム解析系による新規メチル水銀毒性機構の解析 Invited Domestic conference

    笠松真吾

    平成31 年度重金属等による健康影響に関する総合的研究 メチル水銀研究ミーティング  2019.12  環境省

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    Presentation type：Oral presentation (invited, special)  

  • Increased Muscle Mass by Myostatin Deficiency Improves Bacterial Clearance and Survival in Septic Mice International coauthorship International conference

    Masayuki Kobayashi, Shingo Kasamatsu, Shingo Yasuhara, Shohei Shinozaki, Masao Kaneki,

    ASA 2019 – American Society of Anesthesiologists Annual Meeting – Orlando  2019.10  American Society of Anesthesiologists

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  • 活性イオウ分子による親電子シグナル制御系の破綻に起因する有機水銀の神経毒性発現機構 Domestic conference

    笠松真吾，西田基宏，澤　智裕，熊谷嘉人，赤池孝章，居原　秀

    メタルバイオサイエンス研究会2019  2019.10  日本毒性学会 生体金属部会

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  • アルツハイマー病およびてんかんモデルマウスにおける8-nitro-cGMP産生と神経活動への影響 Domestic conference

    居原 秀、岸本祐典、笠松真吾、中嶋秀満、赤池孝章

    第19回日本NO学会学術集会  2019.06  日本NO学会

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  • A novel mechanism for electrophilic cytotoxicity via impairment of reactive persulfide species-regulated redox signaling. International conference

    H. Ihara, S. Kasamatsu, M. Nishida, T. Sawa, Y. Kumagai and T. Akaike

    The 10th International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide  2018.09  Nitric Oxide Society

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  • ニトロソグルタチオン代謝酵素アルコールデヒドロゲナーゼ5のタンパク質ポリスルフィド化による活性制御機構 Domestic conference

    藤井重元、笠松真吾、Alam Md. Morshedul、井田智章、守田匡伸、居原秀、西村明、松永哲郎、本橋ほづみ、赤池孝章

    第18回日本NO学会学術集会  2018.05  日本NO学会

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  • 親電子性物質による活性イオウ分子種依存性レドックスシグナルの破綻 Domestic conference

    北村篤志、笠松真吾、西村明、井田智章、西田基宏、熊谷嘉人、赤池孝章、居原秀

    2017年度生命科学系学会合同年次大会  2017.12  日本生化学会

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  • ニトロソグルタチオン還元酵素（GSNOR）選択的欠損マウスの開発 Domestic conference

    松永哲郎、西村明、笠松真吾、Md. Morshedul Alam、井田智章、守田匡伸、居原秀、藤井重元、下田翔、西田基宏、本橋ほづみ、赤池孝章

    2017年度生命科学系学会合同年次大会  2017.12  日本生化学会

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  • システイニルtRNA合成酵素によるミトコンドリア機能制御の解明 Domestic conference

    松永哲郎、井田智章、魏范研、澤智裕、守田匡伸、西村明、笠松真吾、田沼延公、藤井重元、島礼、居原秀、西田基宏、富澤一仁、本橋ほづみ、赤池孝章

    第70回日本酸化ストレス学会学術集会  2017.06  日本酸化ストレス学会

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  • 酵母におけるシステインパースルフィド合成経路とその生理的役割の解明 Domestic conference

    西村明、那須野亮、松永哲郎、井田智章、笠松真吾、守田匡伸、藤井重元、高木博史、赤池孝章

    第70回日本酸化ストレス学会学術集会  2017.06  日本酸化ストレス学会

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  • アルコールデヒドロゲナーゼ5タンパク質ポリスルフィド化の酵素活性制御機能 Domestic conference

    Jung Minkyung、西村明、笠松真吾、Md. Morshedul Alam、井田智章、松永哲郎、藤井重元、居原秀、本橋ほづみ、赤池孝章

    第70回日本酸化ストレス学会学術集会  2017.06  日本酸化ストレス学会

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  • 硫化水素代謝酵素sulfide quinone reductaseの生体における機能解析 Domestic conference

    守田匡伸、松永哲郎、井田智章、西村明、笠松真吾、藤井重元、赤池孝章

    第70回日本酸化ストレス学会学術集会  2017.06  日本酸化ストレス学会

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  • 細菌性アミノアシルtRNA合成酵素のパースルフィド産生能に関する研究 Domestic conference

    赤司壮一郎、西村明、井田智章、守田匡伸、松永哲郎、笠松真吾、藤井重元、西田基宏、赤池孝章

    第70回日本酸化ストレス学会学術集会  2017.06  日本酸化ストレス学会

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  • 硫化水素/活性イオウ分子種を簡易検出するための抗体の作製 Domestic conference

    居原秀、笠松真吾、赤池孝章

    第70回日本酸化ストレス学会学術集会  2017.06  日本酸化ストレス学会

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  • 酵母におけるシステインパースルフィド合成経路と生理的役割の解明 Domestic conference

    西村明、那須野亮、松永哲郎、井田智章、笠松真吾、守田匡伸、藤井重元、高木博史、赤池孝章

    第17回日本NO学会学術集会  2017.05  日本NO学会

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  • アルコールデヒドロゲナーゼ5の酵素活性制御におけるタンパク質ポリスルフィド化機能 Domestic conference

    井田智章、笠松真吾、Md. Morshedul Alam、守田匡伸、居原秀、西村明、松永哲郎、藤井重元、本橋ほづみ、赤池孝章

    第17回日本NO学会学術集会  2017.05  日本NO学会

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  • 硫化水素代謝酵素Sqrdlの生体における機能解析 Domestic conference

    守田匡伸、南嶋洋司、井田智章、松永哲郎、笠松真吾、西村明、藤井重元、市瀬史、赤池孝章

    第17回日本NO学会学術集会  2017.05  日本NO学会

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  • 細菌から哺乳類に至るまで保存されている硫化水素代謝酵素Sqrdlの遺伝子改変マウスを用いた機能解析 Domestic conference

    守田匡伸、南嶋洋司、井田智章、松永哲郎、笠松真吾、西村明、藤井重元、市瀬史、赤池孝章

    第90回日本細菌学会  2017.03  日本細菌学会

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  • 大腸菌におけるシステインパースルフィド生成機構 Domestic conference

    井田智章、居原秀、守田匡伸、笠松真吾、松永哲郎、西村明、藤井重元、澤智裕、赤池孝章

    第90回日本細菌学会  2017.03  日本細菌学会

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  • アミノアシル-tRNA合成酵素のシステインパースルフィド産生能の検討 Domestic conference

    薗部武、工藤梨沙、西村明、井田智章、赤司壮一郎、ジョン ミンギョン、守田匡伸、松永哲郎、笠松真吾、藤井重元、居原秀、赤池孝章

    第90回日本細菌学会  2017.03  日本細菌学会

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  • 酵母におけるシステインパースルフィドの生理的役割の解明 Domestic conference

    西村明、那須野亮、松永哲郎、井田智章、笠松真吾、守田匡伸、藤井重元、高木博史、赤池孝章

    第90回日本細菌学会  2017.03  日本細菌学会

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  • アルコールデヒドロゲナーゼ5タンパク質ポリスルフィド化の酵素活性制御機能 Domestic conference

    アブドゥラ グラモ チャジア、笠松真吾、西村明、アラム モシェダル、井田智章、松永哲郎、藤井重元、居原秀、本橋ほづみ、赤池孝章

    第90回日本細菌学会  2017.03  日本細菌学会

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  • 8-ニトロ-cGMPとタンパク質poly-S-グアニル化による親電子シグナルの可逆的制御 Domestic conference

    赤司壮一郎、笠松真吾、ジョン ミンギョン、松永哲郎、井田智章、藤井重元、澤智裕、熊谷嘉人、本橋ほづみ、赤池孝章

    第90回日本細菌学会  2017.03  日本細菌学会

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  • 8-メルカプトグアノシン環状一リン酸の免疫細胞化学的検出法の開発 Domestic conference

    北村篤志、松原実咲、笠松真吾、赤池孝章、居原秀

    第90回日本細菌学会  2017.03  日本細菌学会

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  • 硫化水素の簡易検出法の開発 Domestic conference

    藪川啓司、笠松真吾、置田智穂、赤池孝章、居原秀

    第90回日本細菌学会  2017.03  日本細菌学会

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  • 細菌のイオウ呼吸はすべての生物種に保存されている：ほ乳類における新しいエネルギー代謝経路・イオウ呼吸の発見 Domestic conference

    赤池孝章、井田智章、松永哲郎、守田匡伸、笠松真吾、西村明、藤井重元、居原秀、ジョン ミンギョン、赤司壮一郎、澤智裕、本橋ほづみ

    第90回日本細菌学会  2017.03  日本細菌学会

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  • Environmental electrophile impairs reactive persulfide-dependent redox signaling in neurons. International conference

    T. Ida, S. Kasamatsu, H. Ihara and T. Akaike

    Gordon Research Conferences (2017) -Nitric Oxide-   2017.02  Gordon Research Conferences

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  • 生体内ポリサルファー代謝とレドックス制御機能 Invited Domestic conference

    藤井重元、澤智裕、井田智章、笠松真吾、松永哲郎、守田匡伸、赤池孝章

    第89回日本生化学会大会  2016.09  日本生化学会

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  • タンパク質ポリサルファ化の分子メカニズムの解明 Domestic conference

    井田智章、魏研范、笠松真吾、守田匡伸、松永哲郎、居原秀、富澤一仁、熊谷嘉人、澤智裕、本橋ほづみ、赤池孝章

    第89回日本生化学会大会  2016.09  日本生化学会

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  • アルコールデヒドロゲナーゼ5の酵素活性制御におけるタンパク質ポリサルファ化の機能　 Domestic conference

    笠松真吾、Alam Md. Morshedul、井田智章、松永哲朗、藤井重元、居原秀、本橋ほづみ、赤池孝章

    第89回日本生化学会大会  2016.09  日本生化学会

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  • CARS2欠損細胞株の作製とポリサルファ化およびミトコンドリア生合成におけるCARS2の機能解析 Domestic conference

    守田匡伸、井田智章、松永哲郎、笠松真吾、藤井重元、赤池孝章

    第89回日本生化学会大会  2016.09  日本生化学会

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  • Ethylmalonic encephalopathy 1 (ETHE1)による活性イオウ分子種の制御メカニズム Domestic conference

    Jung Minkyung、松永哲郎、笠松真吾、北村大志、小野勝彦、井田智章、澤智裕、藤井重元、本橋ほづみ、赤池孝章

    第89回日本生化学会大会  2016.09  日本生化学会

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  • 酵母をモデル生物とした活性イオウ分子種：システインパースルフィドの生理的役割の解明 Domestic conference

    西村明、那須野亮、松永哲郎、井田智章、笠松真吾、守田匡伸、藤井重元、高木博史、赤池孝章

    第89回日本生化学会大会  2016.09  日本生化学会

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  • 活性イオウ分子種の新しい解析法の構築 Domestic conference

    Abdul Hamid Hisyam、井田智章、笠松真吾、居原秀、土屋幸弘、渡邊泰男、澤智裕、藤井重元、赤池孝章

    第89回日本生化学会大会  2016.09  日本生化学会

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  • ヒトCARS2 KO細胞株を用いたタンパク質ポリサルファ化およびミトコンドリア生合成におけるCARS2の機能解析 Domestic conference

    守田匡伸、井田智章、松永哲郎、笠松真吾、藤井重元、赤池孝章

    第69回日本酸化ストレス学会学術集会  2016.08  日本酸化ストレス学会

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  • 生体内タンパク質ポリスルフィドの検出とその生成機構 Invited Domestic conference

    赤池孝章、笠松真吾

    第69回日本酸化ストレス学会学術集会  2016.08  日本酸化ストレス学会

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  • タンパク質ポリサルファ化によるアルコールデヒドロゲナーゼ5の新規酵素活性制御機構 Domestic conference

    笠松真吾、Md. Morshedul Alam、井田智章、松永哲郎、藤井重元、居原秀、本橋ほづみ、赤池孝章

    第69回日本酸化ストレス学会学術集会  2016.08  日本酸化ストレス学会

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  • 1-Methyl-4-phenylpyridinium（MPP+）誘導性の神経毒性はNO/ROSシグナルにより制御されている Domestic conference

    垣花優希、津々木博康、吉岡寿、桝田くみこ、笠松真吾、井田智章、澤智裕、赤池孝章、居原秀

    第69回日本酸化ストレス学会学術集会  2016.08  日本酸化ストレス学会

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  • 8–Nitro–cGMPによるメチル水銀毒性の調節機構 Domestic conference

    藪川啓司、石崎健勝、北村篤志、笠松真吾、津々木博康、井田智章、藤井重元、澤智裕、赤池孝章、居原秀

    第69回日本酸化ストレス学会学術集会  2016.08  日本酸化ストレス学会

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  • 酵母における活性イオウ分子種:システインパースルフィドの産生とその生理的役割 Domestic conference

    西村明、那須野亮、松永哲郎、井田智章、笠松真吾、守田匡伸、藤井重元、高木博史、赤池孝章

    第69回日本酸化ストレス学会学術集会  2016.08  日本酸化ストレス学会

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  • Ethylmalonic encephalopathy 1（ETHE1）による活性イオウ分子種の制御機構 Domestic conference

    Minkyung Jung、松永哲郎、井田智章、笠松真吾、北村大志、小野勝彦、藤井重元、澤智裕、本橋ほづみ、赤池孝章

    第69回日本酸化ストレス学会学術集会  2016.08  日本酸化ストレス学会

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  • 活性イオウ分子種の新規検出法の開発 Domestic conference

    Hisyam Abdul Hamid、井田智章、笠松真吾、居原秀、土屋幸弘、渡邊泰男、澤智裕、藤井重元、赤池孝章

    第69回日本酸化ストレス学会学術集会  2016.08  日本酸化ストレス学会

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  • Protective function of mitochondrial cysteinyl-tRNA synthetase (CARS2) against electrophiles Domestic conference

    Nur Rahmi Sa'adah、Hisyam Abdul Hamid、井田智章、守田匡伸、松永哲郎、笠松真吾、藤井重元、赤池孝章

    第69回日本酸化ストレス学会学術集会  2016.08  日本酸化ストレス学会

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  • システインtRNA合成酵素欠損細胞株を用いたシステインパースルフィド合成酵素の探索 Domestic conference

    守田匡伸、井田智章、松永哲郎、笠松真吾、西村明、藤井重元、赤池孝章

    生理学研究所研究会「オルガネラネットワークの制御機構とその生理的意義」  2016.07  生理学研究所研究会

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  • システインtRNA合成酵素によるミトコンドリア機能制御 Domestic conference

    松永哲郎、井田智章、魏范研、笠松真吾、西村明幸、守田匡伸、西村明、藤井重元、西田基宏、富澤一仁、赤池孝章

    生理学研究所研究会「オルガネラネットワークの制御機構とその生理的意義」  2016.07  生理学研究所研究会

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  • 酵母をモデル生物としたシステインパースルフィドの生理機能の解明 Domestic conference

    西村明、那須野亮、松永哲郎、井田智章、笠松真吾、守田匡伸、藤井重元、高木博史、赤池孝章

    生理学研究所研究会「オルガネラネットワークの制御機構とその生理的意義」  2016.07  生理学研究所研究会

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  • アミノアシルtRNA合成酵素によるシステインパースルフィド合成とタンパク質ポリサルファ化 Domestic conference

    井田智章、魏研范、居原秀、守田匡伸、笠松真吾、松永哲郎、富澤一仁、澤智裕、本橋ほづみ、赤池孝章

    生理学研究所研究会「オルガネラネットワークの制御機構とその生理的意義」  2016.07  生理学研究所研究会

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  • トレッドミル運動の血中3-ヒドロキシ酪酸濃度に対する影響　 International coauthorship Domestic conference

    渡辺圭一、笠松真吾、神谷具巳、林潤一、金木正夫、石田信彦

    第41回日本運動療法学会  2016.06  日本運動療法学会

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  • Regulatory mechanisms of alcohol dehydrogenase 5 activity via protein polysulfuration. International conference

    S. Kasamatsu, MM Alam, T. Ida, T. Matsunaga, S. Fujii, H. Ihara, H. Motohashi and T. Akaike

    The 9th International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide  2016.05 

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  • Regulation of 1-methyl-4-phenylpyridium ion-indeuced neurotoxicity by nitric oxide/reactive oxygen species signaling. International conference

    Y. Kakihana, H. Tsutsuki, H. Yoshioka, K. Masuda, S. Kasamatsu, T. Ida, T. Sawa, T. Akaike and H. Ihara

    The 9th International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide  2016.05  Nitric Oxide Society

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  • A nobel mechanism for regulating methylmercury-induced cytotoxicity mediated by 8-nitro-cGMP. International conference

    K. Yabukawa, K. Ishizaki, A. Kitamura, S. Kasamatsu, H. Tsutsuki, T. Ida, S. Fujii, T. Sawa, T. Akaike and H. Ihara

    The 9th International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide  2016.05  Nitric Oxide Society

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  • Production of CARS2-knockout human cell lines and functional analysis of their per/polysulfide formation and mitochondrial biogenesis. International conference

    M. Morita, T. Ida, T. Matunaga, S. Kasamatsu, S. Fujii and T. Akaike

    The 9th International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide  2016.05  Nitric Oxide Society

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  • Translation-coupled protein polysulfidaton, a unique biosynthesis pathway of cysteine persulfide. International conference

    T. Ida, H. Ihara, F. Wei, K. Tomizawa, S. Kasamatsu, M. Morita, T. Matsunaga, Y. Kumagai, T. Sawa, H. Motohashi, S. Fujii and T. Akaike

    The 9th International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide  2016.05  Nitric Oxide Society

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  • Development of a new detection system of reactive sulfur species. International conference

    HA. Hamid, T. Ida, S. Kasamatsu, H. Ihara, Y. Tsuchiya, Y. Watanabe, T. Sawa, S. Fujii and T. Akaike

    The 9th International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide  2016.05  Nitric Oxide Society

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  • Mitochondrial cysteinyl tRNA synthase (CARS2): potential protective functions against electrophiles. International conference

    NR. Sa'adah, HA. Hamid, T. Ida, S. Kasamatsu, M. Morita, T. Mastunaga, S. Fujii and T. Akaike

    The 9th International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide  2016.05  Nitric Oxide Society

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  • Protein poly-S-guanylation: Reversible posttranslational modification regulated by protein polysulfur and 8-nitro-cGMP. International conference

    S. Akashi, S. Kasamatsu, M. Jung, T. Matsunaga, T. Ida, S. Fujii, T. Sawa, Y. Kumagai, H. Motohashi and T. Akaike

    The 9th International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide  2016.05  Nitric Oxide Society

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  • Regulatin of mitochondrial biogenesis by cysteine persulfide produced by cysteinyl-tRNA synthetase. International conference

    A. Nishimura, T. Matsunaga, T. Ida, S. Kasamatsu, M. Morita, F. Wei, K. Tomizawa, S. Fujii, H. Motohashi and T. Akaike

    The 9th International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide  2016.05  Nitric Oxide Society

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  • Regulation of mitochondrial; energy metabolism by cysteine persulfide produced by mitochondrial cysteinyl-tRNA synthase. International coauthorship International conference

    F. Sato, M. Okutomi, T. Matsunaga, S. Kasamatsu, M, Kaneki, T. Ida, M. Morita, F. Wei, K. Tomizawa, S. Fujii, H. Motohashi and T. Akaike

    The 9th International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide  2016.05  Nitric Oxide Society

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  • Endogenous reactive sulfur species production and mitochondrial biogenesis by cysteinyl-tRNA synthetase. Invited International conference

    S. Kasamatsu, T. Matsunaga and T. Akaike

    The 9th International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide, Satellite Symposium  2016.05 

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    Presentation type：Oral presentation (invited, special)  

  • Development of metabolome analysis for reactive sulfur species. International conference

    T. Ida, T. Akaike, S. Kasamatsu and S. Fujii

    Gordon Research Conferences (2016) -Oxygen Radicals-  2016.02  Gordon Research Conferences

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  • Translation-coupled protein polysulfuration, a unique biosynthesis pathway of cysteine persulfide. International conference

    S.Kasamatsu, T. Akaike, T. Ida, S. Fujii, H. Ihara and T. Sawa

    Gordon Research Conferences (2016) -Oxygen Radicals-  2016.02  Gordon Research Conferences

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  • タンパク質ポリサルファー解析法 Invited Domestic conference

    笠松真吾、赤司壮一郎、Morshed Alam、本橋ほづみ、赤池孝章

    第3回新学術領域技術支援セミナー  2016.01  新学術領域　「酸素生物学」

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  • タンパク質ポリサルファ化を介したアルコールデヒドロゲナーゼ5の酵素活性制御機構 Domestic conference

    笠松真吾、Md. Morshedul Alam、井田智章、松永哲郎、藤井重元、居原秀、赤池孝章、本橋ほづみ

    BMB2015  2015.12  日本生化学会

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  • 有機水銀の神経毒性発現における8-ニトロ-cGMPの役割と活性イオウ分子による制御 Domestic conference

    藤井重元、笠松真吾、居原秀、津々木博康、石崎健勝、井田智章、澤智裕、熊谷嘉人、赤池孝章

    BMB2015  2015.12  日本生化学会

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  • タンパク質ポリサルファ化の分子メカニズムの解明 Domestic conference

    井田智章、居原秀、魏范研、富澤一仁、長尾翌手可、鈴木勉、熊谷嘉人、澤智裕、笠松真吾、本橋ほづみ、赤池孝章

    BMB2015  2015.12  日本生化学会

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  • 活性イオウ分子種のメタボローム解析法の新たな展開 Domestic conference

    Minkyung Jung、井田智章、笠松真吾、居原秀、藤井重元、赤池孝章

    BMB2015  2015.12  日本生化学会

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  • タンパク質poly-S-グアニル化を介した親電子シグナルの可逆的制御 Domestic conference

    赤司壮一郎、笠松真吾、ジョン ミンギョン、松永哲郎、井田智章、藤井重元、澤智裕、熊谷嘉人、本橋ほづみ、赤池孝章

    BMB2015  2015.12  日本生化学会

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    Presentation type：Poster presentation  

  • 親電子性シグナル制御破綻による有機水銀毒性発現機構 Domestic conference

    笠松真吾、居原秀、津々木博康、石崎健勝、井田智章、藤井重元、澤智裕、熊谷嘉人、赤池孝章

    日本生化学会東北支部第81回例会  2015.09  日本生化学会東北支部

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    Presentation type：Poster presentation  

  • ンパク質ポリチオール化制御機構の解明 Domestic conference

    ヒシャム ビン アブドル ハミル、井田智章、笠松真吾、魏范研、松永哲郎、赤司壮一郎、ジョン ミンギョン、藤井重元、居原秀、澤智裕、富澤一仁、本橋ほづみ、赤池孝章

    日本生化学会東北支部第81回例会  2015.09  日本生化学会東北支部

    　More details

    Presentation type：Poster presentation  

  • システインパースルフィドの新しい検出システムの構築 Domestic conference

    ジョン ミンギョン、井田智章、笠松真吾、松永哲郎、土屋幸弘、渡邊泰男、 藤井重元、赤池孝章

    日本生化学会東北支部第81回例会  2015.09  日本生化学会東北支部

    　More details

    Presentation type：Poster presentation  

  • タンパク質ポリチオール化による親電子シグナル制御 Domestic conference

    赤司壮一郎、笠松真吾、ジョン ミンギョン、松永哲郎、井田智章、藤井重元、本橋ほづみ、澤智裕、熊谷嘉人、赤池孝章

    日本生化学会東北支部第81回例会  2015.09  日本生化学会東北支部

    　More details

    Presentation type：Poster presentation  

  • HPLC-蛍光検出法によるシステインパースルフィドの新しい定量システムの構築 Domestic conference

    ジョン ミンギョン、井田智章、笠松真吾、松永哲郎、土屋幸弘、渡邊泰男、藤井重元、赤池孝章

    第15回日本NO学会学術集会  2015.06  日本NO学会

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    Presentation type：Poster presentation  

  • 活性イオウ分子のNO・活性酸素シグナル制御異常に起因する有機水銀の新規毒性発現機構の解析 Domestic conference

    笠松真吾、居原 秀、津々木博康、石崎健勝、井田智章、藤井重元、澤智裕、熊谷嘉人、赤池孝章

    第15回日本NO学会学術集会  2015.06  日本NO学会

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    Presentation type：Poster presentation  

  • タンパク質Sポリチオール化によるアルコールデヒドロゲナーゼ5の活性制御機構 Domestic conference

    藤井重元、Alam Md. Morshedul、井田智章、松永哲郎、笠松真吾、居原秀、赤池孝章、本橋ほづみ

    第15回日本NO学会学術集会  2015.06  日本NO学会

    　More details

    Presentation type：Poster presentation  

  • グルタチオン依存性ホルムアルデヒド脱水酵素ADH3による酸化ストレス防御と代謝制御　 Domestic conference

    後藤まき、北村大志、Md. Morshedul Alam、長谷場健、山本照子、山本雅之、笠松真吾、井田智章、赤池孝章、本橋ほづみ

    Research PlaNet 2015  2015.06 

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    Presentation type：Poster presentation  

  • 加齢に伴うタンパク質中システインパーサルファイドの変動　 Domestic conference

    藪川啓司、津々木博康、井田智章、笠松真吾、赤池孝章、居原秀

    第15回日本NO学会学術集会  2015.06  日本NO学会

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    Presentation type：Poster presentation  

  • タンパク質S-ポリチール化を介する親電子シグナルの制御メカニズム Domestic conference

    赤司壮一郎、笠松真吾、ジョン ミンギョン、松永哲郎、井田智章、藤井重元、澤智裕、熊谷嘉人、本橋ほづみ、赤池孝章

    第15回日本NO学会学術集会  2015.06  日本NO学会

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    Presentation type：Poster presentation  

  • ポリサルファープロテオーム　 Invited Domestic conference

    笠松真吾

    第2回新学術領域技術支援セミナー  2015.04  新学術領域　「酸素生物学」

    　More details

    Presentation type：Public lecture, seminar, tutorial, course, or other speech  

  • nNOSのSer847リン酸化はNO/ROSレドックスシグナルを制御する Domestic conference

    笠松真吾、渡邊泰男、澤智裕、居原秀、赤池孝章

    第87回日本生化学会大会  2014.10  日本生化学会

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    Presentation type：Poster presentation  

  • nNOSのSer847リン酸化による神経細胞保護機構 Domestic conference

    笠松真吾、渡邊泰男、澤 智裕、居原秀、赤池孝章

    第67回日本酸化ストレス学会学術集会  2014.09  日本酸化ストレス学会

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    Presentation type：Poster presentation  

  • タンパク質ポリサルファー化による酸化ストレス・レドックスシグナル制御 Domestic conference

    藤井重元、井田智章、居原秀、笠松真吾、ジョン・ミンギョン、赤司壮一郎、松永哲郎、津々木博康、澤智裕、本橋ほづみ、熊谷嘉人、赤池孝章

    生理学研究所研究会「新規シグナル伝達分子とその生理学的可能性」  2014.09  生理学研究所研究会

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    Presentation type：Poster presentation  

  • 有機水銀の新規毒性発現機構:活性イオウ分子種のNO/ROSシグナル制御異常 Domestic conference

    津々木博康、笠松真吾、澤智裕、熊谷嘉人、赤池孝章、居原秀

    第14回日本NO学会学術集会  2014.05  日本NO学会

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    Presentation type：Poster presentation  

  • nNOSのSer847リン酸化を介したNO/ROSレドックスシグナル制御 Domestic conference

    笠松真吾、渡邊泰男、澤智裕、赤池孝章、居原秀

    第14回日本NO学会学術集会  2014.05  日本NO学会

    　More details

    Presentation type：Poster presentation  

  • NOシグナルの新規メッセンジャー(8-SH-cGMP)に対するモノクローナル抗体の作製 Domestic conference

    石崎健勝、飯田聡史、笠松真吾、津々木博康、中野長久、赤池孝章、居原秀

    第14回日本NO学会学術集会  2014.05  日本NO学会

    　More details

    Presentation type：Poster presentation  

  • 活性イオウ分子の親電子シグナル制御異常に起因する有機水銀の新規毒性発現機構の解明 Domestic conference

    笠松真吾、津々木博康、居原秀、井田智章、藤井重元、澤智裕、赤池孝章

    第85回日本衛生学会学術総会  2014.03  日本衛生学会

    　More details

    Presentation type：Poster presentation  

  • 活性イオウ分子による有機水銀の解毒代謝機構 Domestic conference

    石崎健勝、笠松真吾、井田智章、藤井重元、熊谷嘉人、赤池孝章、居原秀

    第14回分子予防環境医学研究会  2014.02  分子予防環境医学研究会

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    Presentation type：Poster presentation  

▼display all

Outline of collaborative research (seeds)

  • Sulfur metabolome

Grant-in-Aid for Scientific Research

  • 低温耐性機構における活性硫黄分子種の役割の解明

    2022.04

  • イオウ依存型エネルギー代謝を基盤とした低酸素耐性機構の解析

    2019.04

  • タンパク質ポリスルフィド化を介したレッドクスシグナル制御機構の解明

    2017

  • 活性イオウ分子種を介した細胞内親電子シグナル制御機構の解明

    2015

Incentive donations / subsidies

  • 活性イオウ分子を基軸とした大豆たん白質および関連成分の基礎的研究

    不二たん白質研究振興財団  特定研究  2022.04

  • 網羅的活性イオウ分子検出系（ポリスルフィドミクス）による新規活性イオウ分子の同定とその生理作用の解析

    アサヒグループ学術振興財団  学術研究助成  2021.04

  • 活性イオウ分子を基軸とした大豆たん白質および関連成分の基礎的研究

    不二たん白質研究振興財団  特定研究  2021.04

  • 食肉中に含まれる2-オキソ-イミダゾールジペプチドに関する研究

    伊藤記念財団  研究助成  2020.04

  • 神経細胞におけるニコチンによるレドックスシグナル制御機構

    喫煙科学研究財団  一般研究  2020.04

  • 神経細胞におけるニコチンによるレドックスシグナル制御機構

    喫煙科学研究財団  一般研究  2019.04

▼display all

Charge of on-campus class subject

  • 生物科学特別研究VB

    2023   Intensive lecture   Graduate school

  • 生物科学特別研究VA

    2023   Intensive lecture   Graduate school

  • 生物科学特別演習VB

    2023   Intensive lecture   Graduate school

  • 生物科学特別演習VA

    2023   Intensive lecture   Graduate school

  • 生物科学特別研究IIB

    2023   Intensive lecture   Graduate school

  • 生物科学特別研究IIA

    2023   Intensive lecture   Graduate school

  • 生物科学卒業研究

    2023   Intensive lecture   Undergraduate

  • 生物科学演習II

    2023   Intensive lecture   Undergraduate

  • 生物科学演習I

    2023   Intensive lecture   Undergraduate

  • 生物学実験A

    2023   Weekly class   Undergraduate

  • 生物科学実験II

    2023   Weekly class   Undergraduate

  • 生物化学特別研究4B

    2023   Intensive lecture   Graduate school

  • 生物化学特別研究3B

    2023   Intensive lecture   Graduate school

  • 生物化学特別研究4A

    2023   Intensive lecture   Graduate school

  • 生物化学特別研究3A

    2023   Intensive lecture   Graduate school

  • 生物化学特別演習4B

    2023   Intensive lecture   Graduate school

  • 生物化学特別演習3B

    2023   Intensive lecture   Graduate school

  • 生物化学特別演習4A

    2023   Intensive lecture   Graduate school

  • 生物化学特別演習3A

    2023   Intensive lecture   Graduate school

  • 研究企画ゼミナール2

    2023   Intensive lecture   Graduate school

  • 海外特別研究2

    2023   Intensive lecture   Graduate school

  • 海外特別研究1

    2023   Intensive lecture   Graduate school

  • 生物化学特別研究2B

    2023   Intensive lecture   Graduate school

  • 生物化学特別研究1B

    2023   Intensive lecture   Graduate school

  • 生物化学特別研究2A

    2023   Intensive lecture   Graduate school

  • 生物化学特別研究1A

    2023   Intensive lecture   Graduate school

  • 生物化学特別演習2B

    2023   Intensive lecture   Graduate school

  • 生物化学特別演習1B

    2023   Intensive lecture   Graduate school

  • 生物化学特別演習2A

    2023   Intensive lecture   Graduate school

  • 生物化学特別演習1A

    2023   Intensive lecture   Graduate school

  • 研究企画ゼミナール1

    2023   Intensive lecture   Graduate school

  • 生物化学実験1

    2023   Weekly class   Undergraduate

  • 生物学実験A

    2022   Weekly class   Undergraduate

  • 生物科学実験II

    2022   Weekly class   Undergraduate

  • 研究企画ゼミナールII

    2022   Intensive lecture   Graduate school

  • 生物科学特別研究VB

    2022   Intensive lecture   Graduate school

  • 生物科学特別演習IVB

    2022   Intensive lecture   Graduate school

  • 生物科学特別研究VA

    2022   Intensive lecture   Graduate school

  • 生物科学特別演習IVA

    2022   Intensive lecture   Graduate school

  • 生物科学特別演習IIIA

    2022   Intensive lecture   Graduate school

  • 生物科学特別演習II

    2022   Intensive lecture   Graduate school

  • 生物科学特別演習I

    2022   Intensive lecture   Graduate school

  • 研究企画ゼミナールI

    2022   Intensive lecture   Graduate school

  • 生物科学特別研究IIB

    2022   Intensive lecture   Graduate school

  • 生物科学特別研究IIA

    2022   Intensive lecture   Graduate school

  • 生物科学特別研究IB

    2022   Intensive lecture   Graduate school

  • 生物科学特別研究IA

    2022   Intensive lecture   Graduate school

  • 生物科学卒業研究

    2022   Intensive lecture   Undergraduate

  • 生物科学演習II

    2022   Intensive lecture   Undergraduate

  • 生物科学演習I

    2022   Intensive lecture   Undergraduate

  • 生物科学課題実験

    2022   Intensive lecture   Undergraduate

  • 海外特別研究2

    2022   Intensive lecture   Graduate school

  • 生物化学特別研究3B

    2022   Intensive lecture   Graduate school

  • 生物化学特別研究3A

    2022   Intensive lecture   Graduate school

  • 生物化学特別演習3B

    2022   Intensive lecture   Graduate school

  • 生物化学特別演習3A

    2022   Intensive lecture   Graduate school

  • 研究企画ゼミナール2

    2022   Intensive lecture   Graduate school

  • 海外特別研究1

    2022   Intensive lecture   Graduate school

  • 生物化学特別研究1B

    2022   Intensive lecture   Graduate school

  • 生物化学特別研究1A

    2022   Intensive lecture   Graduate school

  • 生物化学特別演習1B

    2022   Intensive lecture   Graduate school

  • 生物化学特別演習1A

    2022   Intensive lecture   Graduate school

  • 研究企画ゼミナール1

    2022   Intensive lecture   Graduate school

  • 生物科学課題実験

    2021   Intensive lecture   Undergraduate

  • 生物科学演習I

    2021   Intensive lecture   Undergraduate

  • 生物科学卒業研究

    2021   Intensive lecture   Undergraduate

  • 研究企画ゼミナールI

    2021   Intensive lecture   Graduate school

  • 生物科学演習II

    2021   Intensive lecture   Undergraduate

  • 研究企画ゼミナールII

    2021   Intensive lecture   Graduate school

  • 生物科学特別研究IA

    2021   Intensive lecture   Graduate school

  • 生物科学特別研究IB

    2021   Intensive lecture   Graduate school

  • 生物科学特別研究IIA

    2021   Intensive lecture   Graduate school

  • 生物科学特別研究IIB

    2021   Intensive lecture   Graduate school

  • 生物科学特別研究IIIA

    2021   Intensive lecture   Graduate school

  • 生物科学特別研究IIIB

    2021   Intensive lecture   Graduate school

  • 生物科学特別研究IVA

    2021   Intensive lecture   Graduate school

  • 生物科学特別研究IVB

    2021   Intensive lecture   Graduate school

  • 生物科学特別研究VA

    2021   Intensive lecture   Graduate school

  • 生物科学特別研究VB

    2021   Intensive lecture   Graduate school

  • 生物科学特別演習I

    2021   Intensive lecture   Graduate school

  • 生物科学特別演習II

    2021   Intensive lecture   Graduate school

  • 生物科学特別演習IIIA

    2021   Intensive lecture   Graduate school

  • 生物科学特別演習IIIB

    2021   Intensive lecture   Graduate school

  • 生物科学特別演習IVA

    2021   Intensive lecture   Graduate school

  • 生物科学特別演習IVB

    2021   Intensive lecture   Graduate school

  • 生物科学特別演習VA

    2021   Intensive lecture   Graduate school

  • 生物科学実験II

    2021   Weekly class   Undergraduate

  • 生物学実験

    2021   Weekly class   Undergraduate

  • 生物科学特別演習VB

    2021   Intensive lecture   Graduate school

  • 生物科学実験II

    2021   Weekly class   Undergraduate

  • 生物学実験

    2021   Weekly class   Undergraduate

  • 生物科学課題実験

    2020   Intensive lecture   Undergraduate

  • 生物科学演習I

    2020   Intensive lecture   Undergraduate

  • 生物科学卒業研究

    2020   Intensive lecture   Undergraduate

  • 研究企画ゼミナールI

    2020   Intensive lecture   Graduate school

  • 生物科学演習II

    2020   Intensive lecture   Undergraduate

  • 研究企画ゼミナールII

    2020   Intensive lecture   Graduate school

  • 生物科学特別研究IA

    2020   Intensive lecture   Graduate school

  • 生物科学特別研究IB

    2020   Intensive lecture   Graduate school

  • 生物科学特別研究IIA

    2020   Intensive lecture   Graduate school

  • 生物科学特別研究IIB

    2020   Intensive lecture   Graduate school

  • 生物科学特別研究IIIA

    2020   Intensive lecture   Graduate school

  • 生物科学特別研究IIIB

    2020   Intensive lecture   Graduate school

  • 生物科学特別研究IVA

    2020   Intensive lecture   Graduate school

  • 生物科学特別研究IVB

    2020   Intensive lecture   Graduate school

  • 生物科学特別研究VA

    2020   Intensive lecture   Graduate school

  • 生物科学特別研究VB

    2020   Intensive lecture   Graduate school

  • 生物科学特別演習I

    2020   Intensive lecture   Graduate school

  • 生物科学特別演習II

    2020   Intensive lecture   Graduate school

  • 生物科学特別演習IIIA

    2020   Intensive lecture   Graduate school

  • 生物科学特別演習IIIB

    2020   Intensive lecture   Graduate school

  • 生物科学特別演習IVA

    2020   Intensive lecture   Graduate school

  • 生物科学特別演習IVB

    2020   Intensive lecture   Graduate school

  • 生物科学特別演習VA

    2020   Intensive lecture   Graduate school

  • 生物科学特別演習VB

    2020   Intensive lecture   Graduate school

  • 生物学実験

    2020   Weekly class   Undergraduate

  • 生物科学実験II

    2020   Weekly class   Undergraduate

  • 生物科学課題実験

    2019   Intensive lecture   Undergraduate

  • 生物科学演習I

    2019   Intensive lecture   Undergraduate

  • 生物科学卒業研究

    2019   Intensive lecture   Undergraduate

  • 研究企画ゼミナールI

    2019   Intensive lecture   Graduate school

  • 生物科学演習II

    2019   Intensive lecture   Undergraduate

  • 研究企画ゼミナールII

    2019   Intensive lecture   Graduate school

  • 生物科学特別研究IA

    2019   Intensive lecture   Graduate school

  • 生物科学特別研究IB

    2019   Intensive lecture   Graduate school

  • 生物科学特別研究IIA

    2019   Intensive lecture   Graduate school

  • 生物科学特別研究IIB

    2019   Intensive lecture   Graduate school

  • 生物科学特別研究IIIA

    2019   Intensive lecture   Graduate school

  • 生物科学特別研究IIIB

    2019   Intensive lecture   Graduate school

  • 生物科学特別研究IVA

    2019   Intensive lecture   Graduate school

  • 生物科学特別研究IVB

    2019   Intensive lecture   Graduate school

  • 生物科学特別研究VA

    2019   Intensive lecture   Graduate school

  • 生物科学特別研究VB

    2019   Intensive lecture   Graduate school

  • 生物科学特別演習I

    2019   Intensive lecture   Graduate school

  • 生物科学特別演習II

    2019   Intensive lecture   Graduate school

  • 生物科学特別演習IIIA

    2019   Intensive lecture   Graduate school

  • 生物科学特別演習IIIB

    2019   Intensive lecture   Graduate school

  • 生物科学特別演習IVA

    2019   Intensive lecture   Graduate school

  • 生物科学特別演習IVB

    2019   Intensive lecture   Graduate school

  • 生物科学特別演習VA

    2019   Intensive lecture   Graduate school

  • 生物科学特別演習VB

    2019   Intensive lecture   Graduate school

  • 生物科学実験II

    2019   Intensive lecture   Undergraduate

▼display all

Charge of off-campus class subject

  • 環境医学

    2023.04
    -
    2024.03
    Institution：Tohoku University

    　More details

    Country：Japan

  • 環境医学

    2022.04
    -
    2023.03
    Institution：Tohoku University

    　More details

    Country：Japan

  • 環境医学

    2021.04
    -
    2022.03
    Institution：Tohoku University

    　More details

    Country：Japan

  • 環境医学

    2020.04
    -
    2021.03
    Institution：Tohoku University

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    Country：Japan

  • 環境医学

    2019.04
    -
    2020.03
    Institution：Tohoku University

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    Country：Japan

Number of papers published by graduate students

  • 2022

    Number of undergraduate student / college student presentations：0  Number of graduate students presentations：4

  • 2021

    Number of undergraduate student / college student presentations：0  Number of graduate students presentations：3

  • 2020

    Number of undergraduate student / college student presentations：0  Number of graduate students presentations：1

Number of instructed thesis, researches

  • 2022

    Number of instructed the graduation thesis：4  Number of graduation thesis reviews：0

    [Number of instructed the Master's Program] (previous term)：4  [Number of instructed the Master's Program] (letter term)：0

    [Number of master's thesis reviews] (chief)：0  [Number of master's thesis reviews] (vice-chief)：0

    [Number of doctoral thesis reviews] (chief)：0  [Number of doctoral thesis reviews] (vice-chief)：0

  • 2021

    Number of instructed the graduation thesis：5  Number of graduation thesis reviews：0

    [Number of instructed the Master's Program] (previous term)：3  [Number of instructed the Master's Program] (letter term)：0

    [Number of master's thesis reviews] (chief)：0  [Number of master's thesis reviews] (vice-chief)：0

    [Number of doctoral thesis reviews] (chief)：0  [Number of doctoral thesis reviews] (vice-chief)：0

  • 2020

    Number of instructed the graduation thesis：5  Number of graduation thesis reviews：0

    [Number of instructed the Master's Program] (previous term)：3  [Number of instructed the Master's Program] (letter term)：1

    [Number of master's thesis reviews] (chief)：0  [Number of master's thesis reviews] (vice-chief)：0

    [Number of doctoral thesis reviews] (chief)：0  [Number of doctoral thesis reviews] (vice-chief)：0

  • 2019

    Number of instructed the graduation thesis：3  Number of graduation thesis reviews：0

    [Number of instructed the Master's Program] (previous term)：2  [Number of instructed the Master's Program] (letter term)：0

    [Number of master's thesis reviews] (chief)：0  [Number of master's thesis reviews] (vice-chief)：0

    [Number of doctoral thesis reviews] (chief)：0  [Number of doctoral thesis reviews] (vice-chief)：0

Academic Activities

  • Free Radical Research

    Role(s)： Peer review

    2022.04 - Now

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    Type：Peer review 

    審査件数：1件

  • Life

    Role(s)： Peer review

    2022.04 - Now

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    Type：Peer review 

    審査件数：1件

  • Antioxidants

    Role(s)： Peer review

    2020.10 - Now

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    Type：Peer review 

    審査件数：5件

  • Nitric Oxide

    Role(s)： Peer review

    2020.04 - Now

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    Type：Peer review 

    審査件数：2件

  • International Journal of Molecular Sciences

    Role(s)： Peer review

    2020.04 - Now

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    Type：Peer review 

    審査件数：2件

  • The Journal of Biochemistry

    Role(s)： Peer review

    2019.04 - 2020.03

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    Type：Peer review 

    審査件数：1件

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