Updated on 2024/03/26

写真a

 
Ishikawa Shingo
 
Organization
Graduate School of Veterinary Science Department of Veterinary Science Associate Professor
School of Veterinary Science Department of Veterinary Science
Title
Associate Professor
Affiliation
Institute of Veterinary Science
Affiliation campus
Rinku Campus

Position

  • Graduate School of Veterinary Science Department of Veterinary Science 

    Associate Professor  2022.04 - Now

  • School of Veterinary Science Department of Veterinary Science 

    Associate Professor  2022.04 - Now

Degree

  • 獣医学博士 ( Nippon Veterinary and Life Science University )

  • 獣医学学士 ( Kitasato University )

Research Areas

  • Life Science / Animal life science

  • Life Science / Immunology

  • Life Science / Veterinary medical science

Research Interests

  • 自然免疫

  • 粘膜免疫

  • 牛呼吸器病症候群

  • immunology

  • 予防獣医学

  • macrophage

  • equine mesenchymal stem cell

  • Bovine Respiratory Disease Complex

  • innate immunology

Research subject summary

  • 牛呼吸器病症候群の病態解明と新規予防法の検討

Professional Memberships

  • ウマ科学会

  • 家畜感染症学会

  • 日本家畜臨床学会

  • 日本獣医学会

  • 日本獣医師会(日本産業動物獣医学会)

Awards

  • 九州地区学会賞

    石川真悟, 宮澤昌嵩, 田中千穂, 地挽良典, 古賀靖啓, 津曲圭太, 林淳, 濱野剛久, 帆保誠二

    2019.11   第68回九州地区獣医師大会  

Job Career (off-campus)

  • Osaka Metropolitan University   Graduate School of Veterinary Science

    2022.04 - Now

  • Osaka Prefecture University   Graduate School of Life and Environmental Sciences Division of Veterinary Science

    2021.04 - 2022.03

  • Kagoshima University   Joint faculty of Veterinary Medicine Department of Veterinary Medicine

    2015.04 - 2021.03

Papers

  • Effects of trastuzumab emtansine on canine urothelial carcinoma cells in vitro and in vivo.

    Sakai K, Kato D, Yoshinaka J, Takahashi Y, Ikeda N, Aoki S, Iguchi T, Ishikawa S, Yamagishi N, Shimamura S, Nakagawa T

    Veterinary and comparative oncology   2024.03( ISSN:1476-5810

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  • Case report: Measuring the Circulating Bone Metabolic Markers of Two Asian Elephant (<i>Elephas maximus</i>) Calves Around Weaning

    TAKEHANA Kazuya, ADACHI Mare, ISHIKAWA Shingo, YAMAGISHI Norio

    Japanese Journal of Zoo and Wildlife Medicine   28 ( 2 )   115 - 120   2023.09( ISSN:13426133

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    <p> We measured the serum activities of the bone-specific isoenzyme of alkaline phosphatase (ALP3) and tartrate-resistant acid phosphatase 5b (TRAP5b), as osteoblastic bone formation and osteoclastic bone resorption markers, respectively, in two zoo-managed Asian elephant calves during the 4-month period around weaning (2 months before and after weaning). The serum ALP3 and TRAP5b levels decreased after weaning; the levels were significantly higher in the pre-than post-weaning period. These results suggest that osteoblastic bone formation and osteoclastic bone resorption slowed after weaning. Breast milk feeding of Asian elephants may play a significant role in osteoblastic and osteoclastic bone metabolism during the growth period.</p>

    DOI: 10.5686/jjzwm.28.115

  • 症例報告 アジアゾウ(Elephas maximus)の子ゾウ2頭における離乳前後の血中骨代謝マーカー値(Case report: Measuring the Circulating Bone Metabolic Markers of Two Asian Elephant(Elephas maximus) Calves Around Weaning)

    竹鼻 一也, 安達 希, 石川 真悟, 山岸 則夫

    日本野生動物医学会誌   28 ( 2 )   115 - 120   2023.09( ISSN:1342-6133

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    アジアゾウの子ゾウ2頭の離乳前後4ヵ月における骨芽細胞マーカー[骨型アルカリホスファターゼ(ALP3)]および破骨細胞マーカー[酒石酸耐性酸ホスファターゼ5b(TRAP5b)]の活性値を測定した.両測定値は離乳後に有意に減少したことから,骨芽細胞による骨形成と破骨細胞による骨吸収の低下が示唆された。以上より,アジアゾウの母乳は成長期の骨代謝に重要な役割を持つことが推察された。(著者抄録)

  • 動物園で管理したアジアゾウ(Elephas maximus)の血清クレアチニンキナーゼと乳酸脱水素酵素のアガロースゲル電気泳動のパターン(Agarose gel electrophoresis pattern of serum creatine kinase and lactate dehydrogenase isoenzymes in zoo-managed Asian elephants(Elephas maximus))

    Takehana Kazuya, Adachi Mare, Ishikawa Shingo, Yamagishi Norio

    The Journal of Veterinary Medical Science   85 ( 5 )   578 - 583   2023.05( ISSN:0916-7250

  • 健康ブタの気管支肺胞領域内におけるenrofloxacinの分布状況(Distribution of enrofloxacin within the bronchoalveolar region of healthy pigs)

    Ijiri Moe, Ishikawa Shingo, Hobo Seiji

    The Journal of Veterinary Medical Science   85 ( 3 )   296 - 300   2023.03( ISSN:0916-7250

  • 動物園管理のアジアゾウ(Elephas maximus)における2種の認証された方法を用いた血清中アルカリホスファターゼのイソ酵素の活性(The serum activities of alkaline phosphatase isoenzymes measured using two approved methods in zoo-managed Asian elephants(Elephas maximus))

    Takehana Kazuya, Adachi Mare, Ishikawa Shingo, Yamagishi Norio

    The Journal of Veterinary Medical Science   85 ( 2 )   232 - 235   2023.02( ISSN:0916-7250

  • Agarose gel electrophoresis pattern of serum creatine kinase and lactate dehydrogenase isoenzymes in zoo-managed Asian elephants (<i>Elephas maximus</i>)

    TAKEHANA Kazuya, ADACHI Mare, ISHIKAWA Shingo, YAMAGISHI Norio

    Journal of Veterinary Medical Science   85 ( 5 )   578 - 583   2023( ISSN:09167250

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    <p>Serum levels of creatine kinase (CK) and lactate dehydrogenase (LDH) isoenzymes were evaluated in nine zoo-managed Asian elephants (<i>Elephas maximus</i>) using a commercial agarose gel electrophoresis (AGE) kit. CK was separated into two major fractions, CK-BB and CK-MM, along with a small fraction of macroenzyme-CK type 2 (mCK2); CK-MM was the largest fraction. LDH was separated into five fractions (LDH1–5); LDH3 was the largest fraction. Age was negatively and positively correlated with the percentages of CK-BB and CK-MM, respectively, and negatively correlated with CK-BB and mCK2 activities. These results indicate that an AGE kit can be used to evaluate CK and LDH isoenzymes. Routine isoenzyme testing may enable early detection of disease and physiological changes.</p>

    DOI: 10.1292/jvms.22-0550

    PubMed

  • The serum activities of alkaline phosphatase isoenzymes measured using two approved methods in zoo-managed Asian elephants (<i>Elephas maximus</i>)

    TAKEHANA Kazuya, ADACHI Mare, ISHIKAWA Shingo, YAMAGISHI Norio

    Journal of Veterinary Medical Science   85 ( 2 )   232 - 235   2023( ISSN:09167250

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    <p>The approved Japanese measurement method of circulating alkaline phosphatase (ALP) has changed from that of the Japan Society of Clinical Chemistry (JSCC) to that of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC). We measured the serum levels of total ALP (t-ALP) and those of the isoenzymes ALP2 and ALP3 in 50 Asian elephant (<i>Elephas maximus</i>) specimens using both methods. The activities determined by the IFCC method were roughly one-third lower than those determined by the JSCC method. We present conversion formulae. Our results enable comparisons of historical and current data on serum ALP activities in endangered, zoo-managed Asian elephants.</p>

    DOI: 10.1292/jvms.22-0440

    PubMed

  • Distribution of enrofloxacin within the bronchoalveolar region of healthy pigs

    IJIRI Moe, ISHIKAWA Shingo, HOBO Seiji

    Journal of Veterinary Medical Science   85 ( 3 )   296 - 300   2023( ISSN:09167250

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    <p>This study aimed to elucidate the distribution of enrofloxacin (ERFX) within the bronchoalveolar region of pigs. Six clinically healthy pigs were allocated to intramuscular treatments with either a single dose of 5 mg/kg or 7.5 mg/kg ERFX. Samples of plasma and bronchoalveolar lavage fluid (BALF) were obtained from each pig 0 (before administration), 3, 8, and 24 hr after ERFX administration. The ERFX concentrations in pulmonary epithelial lining fluid (ELF) and BALF cells showed a similar pattern during the experimental period, whereby ERFX concentrations in both ELF and BALF cells were higher than those in the plasma. These results suggest that intramuscularly injected ERFX is well-distributed in the bronchoalveolar region.</p>

    DOI: 10.1292/jvms.22-0416

    PubMed

  • Flow cytometric analysis of bronchoalveolar lavage fluid immune dynamics in calves.

    Shingo Ishikawa, Masataka Miyazawa, Yoshinori Zibiki, Rie Kamikakimoto, Seiji Hobo

    The Journal of veterinary medical science   84 ( 4 )   548 - 557   2022.04( ISSN:0916-7250

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:Domestic journal  

    Understanding the immune dynamics in the respiratory mucosa of calves is necessary for a good management of bovine respiratory disease. Immune dynamics in the respiratory mucosa in humans and experimental animals has been assessed by flow cytometric analysis of bronchoalveolar lavage fluid (BALF); however, few reports have addressed this subject in calves. The aim of this study was to establish a universal method to analyze bronchoalveolar lavage fluid (BALF) by flow cytometry and to obtain basic knowledge of bovine respiratory mucosal immune dynamics. We investigated the immune cell populations in BALF and evaluated the surface antigen expression of alveolar macrophages in calves using flow cytometer. To further analyze the surface antigen variation observed in alveolar macrophages in detail, stimulation assays were performed in vitro. BALF cells were separated into three distinct populations based on their light scatter plot, which were considered to be macrophages, lymphocytes, and neutrophils. In most individuals, most of the BALF immune cells were alveolar macrophages, but an increased proportion of lymphocytes and neutrophils was observed in some individuals. Analysis of each surface antigen expression in alveolar macrophages showed that CD21 and MHC class II expression changed in response to changes in the leukocyte population. Moreover, when alveolar macrophages were stimulated with interferon-γ in vitro, the expression of CD21 was drastically reduced and MHC class II was increased, suggesting that functional changes in alveolar macrophages themselves are involved in the immune dynamics.

    DOI: 10.1292/jvms.21-0522

    PubMed

  • 仔牛の気管支肺胞洗浄液中の免疫動態についてのフローサイトメトリ分析(Flow cytometric analysis of bronchoalveolar lavage fluid immune dynamics in calves)

    Ishikawa Shingo, Miyazawa Masataka, Zibiki Yoshinori, Kamikakimoto Rie, Hobo Seiji

    The Journal of Veterinary Medical Science   84 ( 4 )   548 - 557   2022.04( ISSN:0916-7250

  • Pharmacokinetics in plasma and alveolar regions of a healthy calf intramuscularly administered a single dose of orbifloxacin.

    Tetsuro Kuramae, Shingo Ishikawa, Aki Kono, Seiji Hobo

    The Journal of veterinary medical science   84 ( 3 )   400 - 405   2022.03( ISSN:0916-7250

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:Domestic journal  

    This study analyzed the pharmacokinetics of orbifloxacin (OBFX) in plasma, and its migration and retention in epithelial lining fluid (ELF) and alveolar cells within the bronchoalveolar lavage fluid (BALF). Four healthy calves received a single dose of OBFX (5.0 mg/kg) intramuscularly. Post-administration OBFX dynamics were in accordance with a non-compartment model, including the absorption phase. The maximum concentration (Cmax) of plasma OBFX was 2.2 ± 0.1 μg/ml at 2.3 ± 0.5 hr post administration and gradually decreased to 0.3 ± 0.2 μg/ml at 24 hr following administration. The Cmax of ELF OBFX was 9.3 ± 0.4 μg/ml at 3.0 ± 2.0 hr post administration and gradually decreased to 1.2 ± 0.1 μg/ml at 24 hr following administration. The Cmax of alveolar cells OBFX was 9.3 ± 2.9 μg/ml at 4.0 hr post administration and gradually decreased to 1.1 ± 0.2 μg/ml at 24 hr following administration. The half-life of OBFX in plasma, ELF, and alveolar cells were 6.9 ± 2.2, 7.0 ± 0.6, and 7.8 ± 1.6 hr, respectively. The Cmax and the area under the concentration-time curve for 0-24 hr with OBFX were significantly higher in ELF and alveolar cells than in plasma (P<0.05). These results suggest that OBFX is distributed and retained at high concentrations in ELF and alveolar cells at 24 hr following administration. Hence, a single intramuscular dose of OBFX (5.0 mg/kg) may be an effective therapeutic agent against pneumonia.

    DOI: 10.1292/jvms.21-0517

    PubMed

  • 健康な仔牛の一例に対する単回の筋内orbifloxacin投与後の血漿と肺胞領域における薬物動態の解析(Pharmacokinetics in plasma and alveolar regions of a healthy calf intramuscularly administered a single dose of orbifloxacin)

    Kuramae Tetsuro, Ishikawa Shingo, Kono Aki, Hobo Seiji

    The Journal of Veterinary Medical Science   84 ( 3 )   400 - 405   2022.03( ISSN:0916-7250

  • Interferon gamma, lipopolysaccharide, and modified-live viral vaccines stimulation alter the mRNA expression of tumor necrosis factor α, inducible nitric oxide synthase, and interferon β in bovine alveolar macrophages.

    Shingo Ishikawa, Masataka Miyazawa, Chiho Tanaka, Ryoma Uesawa, Juri Nishizawa, Ryoko Uemura, Ikuo Kobayashi, Seiji Hobo

    Veterinary immunology and immunopathology   244   110378 - 110378   2022.02( ISSN:0165-2427

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    To understand the pathogenesis of bovine respiratory disease (BRD), it is necessary to elucidate the mechanisms of alveolar macrophage regulation by cytokines and pathogen-associated molecular patterns (PAMPs). Moreover, "non-specific effects (NSEs)" an innate immune regulatory mechanism in response to vaccines containing PAMPs, has recently attracted attention. It may be applied to BRD control, but there is limited knowledge in bovine. To investigate this, we stimulated alveolar macrophages in vitro with lipopolysaccharide (LPS), polyinosinic-polycytidylic acid sodium salt (Poly I:C), interferon gamma (IFN-γ), and modified-live viral (MLV) vaccines, respectively, and analyzed changes in tumor necrosis factor alpha (TNF-α), inducible nitric oxide synthase (iNOS), and interferon beta (IFN-β) mRNA expression levels. mRNA expression levels of TNF-α, iNOS, and IFN-β were significantly increased in bovine alveolar macrophages stimulated by IFN-γ and MLV vaccine; LPS, IFN-γ, and MLV vaccine; and MLV vaccine only, respectively. Additionally, all MLV vaccine-stimulated mRNA expression increases were observed in a concentration-dependent manner. These results revealed in part, the mechanism of bovine alveolar macrophage regulation by cytokines and PAMPs. Understanding the regulatory mechanisms of alveolar macrophages will contribute to understanding the pathogenesis of BRD and preventive and therapeutic BRD management based on NSEs.

    DOI: 10.1016/j.vetimm.2021.110378

    PubMed

  • Osteochondral regeneration of the femoral medial condyle by using a scaffold-free 3D construct of synovial membrane-derived mesenchymal stem cells in horses.

    Daiki Murata, Shingo Ishikawa, Takafumi Sunaga, Yasuo Saito, Takeshi Sogawa, Koichi Nakayama, Seiji Hobo, Takashi Hatazoe

    BMC veterinary research   18 ( 1 )   53 - 53   2022.01

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    BACKGROUND: Medical interventions for subchondral bone cysts in horses have been extensively studied. This study investigated the regeneration of articular cartilage and subchondral bone with scaffold-free three-dimensional (3D) constructs of equine synovial membrane-derived mesenchymal stem cells (SM-MSCs) isolated from three ponies and expanded until over 1.0 × 107 cells at passage 2 (P2). RESULTS: SM-MSCs were strongly positive for CD11a/CD18, CD44, and major histocompatibility complex (MHC) class I; moderately positive for CD90, CD105, and MHC class II; and negative for CD34 and CD45 on flow cytometry and differentiated into osteogenic, chondrogenic, and adipogenic lineages in the tri-lineage differentiation assay. After culturing SM-MSCs until P3, we prepared a construct (diameter, 6.3 mm; height, 5.0 mm) comprising approximately 1920 spheroids containing 3.0 × 104 cells each. This construct was confirmed to be positive for type I collagen and negative for type II collagen, Alcian blue, and Safranin-O upon histological analysis and was subsequently implanted into an osteochondral defect (diameter, 6.8 mm; depth, 5.0 mm) at the right femoral medial condyle. The contralateral (left femoral) defect served as the control. At 3 and 6 months after surgery, the radiolucent volume (RV, mm3) of the defects was calculated based on multiplanar reconstruction of computed tomography (CT) images. Magnetic resonance (MR) images were evaluated using a modified two-dimensional MR observation of cartilage repair tissue (MOCART) grading system, while macroscopic (gross) and microscopic histological characteristics were scored according to the International Cartilage Repair Society (ICRS) scale. Compared to the control sites, the implanted defects showed lower RV percentages, better total MOCART scores, higher average gross scores, and higher average histological scores. CONCLUSIONS: Implantation of a scaffold-free 3D-construct of SM-MSCs into an osteochondral defect could regenerate the original structure of the cartilage and subchondral bone over 6 months post-surgery in horses, indicating the potential of this technique in treating equine subchondral bone cysts.

    DOI: 10.1186/s12917-021-03126-y

    PubMed

  • Bovine Respiratory Disease Complex: Causative Organisms and Causative Viruses in Bronchoalveolar Regions of Introduced Fattening Cattle and Their Treatment Status after Introduction

    HAYASHI Jun, ISHIKAWA Shingo, TSUMAGARI Keita, KURAMAE Tetsuro, HOBO Seiji

    Journal of the Japan Veterinary Medical Association   74 ( 8 )   497 - 502   2021.08( ISSN:0446-6454

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    We investigated bovine respiratory disease complex by collecting bronchoalveolar lavage fluid (BALF) for bacteriological and virological examinations from fattening cattle following their introduction from market, and monitored their treatment records for any subsequent history of medical interventions. A cohort of 10 steers underwent BALF collection in April 2016, and <i>Pasteurella multocida</i> and <i>Mycoplasma bovis</i> were isolated in samples from eight and one animal, respectively, in bacteriology, and <i>Respiratory Syncytial</i> (RS) virus genetic material was detected in a sample from one animal in virology. A second cohort of 10 steers from the same herd underwent BALF collection in December 2016, and <i>Pasteurella multocida</i> and <i>Mycoplasma bovis</i> were isolated in samples from four and one animal, respectively, in bacteriology, and RS virus genetic material was detected in samples from all 10 animals in virology. Monitoring post-introduction treatment showed that the cohort with ubiquitously detected RS virus tended to require earlier medical interventions. These findings indicate the presence of pneumonia-causing bacteria or viruses in the bronchoalveolar region of cattle after introduction, which may be important for the development of preventive measures and treatment strategies.

    DOI: 10.12935/jvma.74.497

    CiNii Article

    Other URL: http://id.ndl.go.jp/bib/031657317

  • 導入肥育素牛の気管支肺胞領域に認められた牛呼吸器病症候群原因菌及び牛RSウイルスと導入後の治療状況

    林 淳, 石川 真悟, 津曲 圭太, 藏前 哲郎, 帆保 誠二

    日本獣医師会雑誌   74 ( 8 )   497 - 502   2021.08( ISSN:0446-6454

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    市場導入肥育素牛から気管支肺胞洗浄液(BALF)を採取し、微生物学的に検査するとともに、供試牛の導入後治療状況を調査した。BALFからの細菌分離検査では、Pasteurella multocida及びMycoplasma bovisが分離された。また、ウイルス遺伝子検査では牛Respiratory syncytial(RS)ウイルス遺伝子が検出された。供試牛の導入後治療状況を調査した結果、牛RSウイルス遺伝子が全頭に確認された牛群では導入後に牛呼吸器病症候群治療を受けるまでの期間が早かった。これらの知見は、導入後の牛の気管支肺胞領域に肺炎原因菌あるいは原因ウイルスが存在することを示しており、予防としてのワクチネーションの徹底や使用する抗菌剤の的確な選定を含め、治療方針の決定に重要であると考えられた。(著者抄録)

  • Primary sinonasal malignant melanoma with systemic metastasis in a non-gray horse.

    Hitoshi Hatai, Takashi Hatazoe, Haruka Seo, Teruaki Tozaki, Shingo Ishikawa, Noriaki Miyoshi, Kazuhiro Misumi, Seiji Hobo

    Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc   33 ( 2 )   379 - 383   2021.03( ISSN:1040-6387

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    A 27-y-old Anglo-Arabian gelding with bay coat color was presented with a swelling of the left maxillary region. Fenestration on the left maxilla revealed that the left maxillary sinus was filled with black-red tissue. A portion of the tissue was excised and diagnosed histologically as malignant melanoma. Genotyping of the STX17 gene for gray coat color revealed that the horse did not have the "gray" factor. The horse was euthanized ~3 mo after first presentation. During autopsy, a black-to-gray mass extended from the left nasal cavity to the surrounding paranasal sinus and invaded the hard palate, cribriform plate, and the cranial portion of the left olfactory bulb. Moreover, identical black nodules were present in lymph nodes from the mandible to the larynx, and in the spleen, liver, kidney, and adrenal glands. However, masses were not found in the skin, perineal region, or pelvic cavity. All of the black-to-gray nodules were malignant melanomas that were histologically identical to the initial biopsy; tumor emboli were also found in the kidney. Sinonasal mucosal melanoma is a rare disease in horses.

    DOI: 10.1177/1040638720978561

    PubMed

  • Interferon gamma, lipopolysaccharide, and modified-live viral vaccines stimulation alter the mRNA expression of tumor necrosis factor α, inducible nitric oxide synthase, and interferon β in bovine alveolar macrophages Reviewed

    Shingo Ishikawa, Masataka Miyazawa, Chiho Tanaka, Ryoma Uesawa, Juri Nishizawa, Ryoko Uemura, Ikuo Kobayashi, Seiji Hobo

    Veterinary Immunology and Immunopathology   2021

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    Kind of work:Joint Work  

    Repository URL: http://hdl.handle.net/10466/00017577

  • Pharmacokinetics in plasma and alveolar regions of healthy calves subcutaneously administered a single dose of enrofloxacin.

    Tetsuro Kuramae, Konosuke Otomaru, Masaya Hirata, Shingo Ishikawa, Michiko Noguchi, Tomonobu Ikedo, Chie Horinouchi, Jun Hayashi, Keita Tsumagari, Seiji Hobo

    The Journal of veterinary medical science   82 ( 8 )   1197 - 1203   2020.08( ISSN:0916-7250

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:Domestic journal  

    This study aimed to analyze the pharmacokinetics of enrofloxacin (ERFX) and its metabolite ciprofloxacin (CPFX) in plasma, as well as their migration to, and retention in, the epithelial lining fluid (ELF) and alveolar cells within the bronchoalveolar fluid (BALF). Four healthy calves were subcutaneously administered a single dose of ERFX (5 mg/kg). ERFX and CPFX dynamics post-administration were analyzed via a non-compartment model, including the absorption phase. The Cmax of plasma ERFX was 1.6 ± 0.4 µg/ml at 2.3 ± 0.5 hr post-administration and gradually decreased to 0.14 ± 0.03 µg/ml at 24 hr following administration. The mean residence time between 0 and 24 hr (MRT0-24) in plasma was 6.9 ± 1.0 hr. ERFX concentrations in ELF and alveolar cells peaked at 3.0 ± 2.0 hr and 4.0 ± 2.3 hr following administration, respectively, and gradually decreased to 0.9 ± 0.8 µg/ml and 0.8 ± 0.5 µg/ml thereafter. The plasma half-life (t1/2) of ERFX was 6.5 ± 0.7 hr, while that in ELF and alveolar cells was 6.5 ± 3.6 and 7.4 ± 4.3 hr, respectively. The Cmax and the area under the concentration-time curve for 0-24 hr for ERFX were significantly higher in alveolar cells than in plasma (P<0.05). These results suggest that ERFX is distributed at high concentrations in ELF and is retained at high concentrations in alveolar cells after 24 hr in the BALF region; hence, ERFX may be an effective therapeutic agent against pneumonia.

    DOI: 10.1292/jvms.20-0110

    PubMed

  • Distribution of marbofloxacin within the bronchoalveolar region of healthy pigs.

    Moe Ijiri, Shingo Ishikawa, Yoshinori Jibiki, Masataka Miyazawa, Akane Senokuchi, Seiji Hobo

    The Journal of veterinary medical science   82 ( 8 )   1080 - 1083   2020.08( ISSN:0916-7250

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:Domestic journal  

    The purpose of this study was to clarify the distribution of marbofloxacin (MBFX) within the bronchoalveolar region of pigs. Four clinically healthy pigs were intramuscularly injected with a single dose of MBFX (2 mg/kg). Samples of plasma and bronchoalveolar lavage fluid (BALF) were obtained for each pig at 0 (before administration), 3, 8 and 24 hr after administration of MBFX. As a result, the MBFX concentrations in pulmonary epithelial lining fluid (ELF) and in alveolar cells showed a similar pattern of concentrations during the experimental period. The MBFX concentrations both in ELF and alveolar cells were higher than in plasma. These results suggest that intramuscularly injected MBFX was well distributed in the bronchoalveolar region.

    DOI: 10.1292/jvms.20-0239

    PubMed

  • エンロフロキサシンを単回皮下投与した健康仔牛の血漿中及び肺胞領域の薬物動態(Pharmacokinetics in plasma and alveolar regions of healthy calves subcutaneously administered a single dose of enrofloxacin)

    Kuramae Tetsuro, Otomaru Konosuke, Hirata Masaya, Ishikawa Shingo, Noguchi Michiko, Ikedo Tomonobu, Horinouchi Chie, Hayashi Jun, Tsumagari Keita, Hobo Seiji

    The Journal of Veterinary Medical Science   82 ( 8 )   1197 - 1203   2020.08( ISSN:0916-7250

  • 健康な豚の気管支肺胞領域内におけるマルボフロキサシンの分布(Distribution of marbofloxacin within the bronchoalveolar region of healthy pigs)

    Ijiri Moe, Ishikawa Shingo, Jibiki Yoshinori, Miyazawa Masataka, Senokuchi Akane, Hobo Seiji

    The Journal of Veterinary Medical Science   82 ( 8 )   1080 - 1083   2020.08( ISSN:0916-7250

  • 黒毛和種仔ウシの末梢血白血球に及ぼすβカロテン投与の影響(Effect of Beta-Carotene Supplementation on the Peripheral Blood Leukocyte Population in Japanese Black Calves)

    Otomaru Konosuke, Ogawa Rei, Oishi Shoko, Iwamoto Yuki, Ishikawa Shingo, Nagai Katsuhisa

    Journal of Nutritional Science and Vitaminology   66 ( 4 )   381 - 385   2020.08( ISSN:0301-4800

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    2週齢の黒毛和種仔ウシ(n=11)にβカロテン20mg/日を8週齢まで経口投与し、末梢血白血球への影響を調べた。投与群の血清βカロテン濃度は、4、8、12週齢時に非投与群に比べ有意に高値を示した。血清レチノール濃度に群間差はなく、2週齢時から12週齢時まで両群とも類似の漸増パターンを示した。このことから、βカロテン20mg/日を黒毛和種仔ウシに投与しても、血清レチノール濃度に大きな影響はないと考えられた。末梢血の総白血球数、単核球数、顆粒球数、およびCD3陽性(+)、CD8+、CD14+、CD21+、CD335+細胞数に有意な群間差はみられず、有意な経時変化も認められなかった。末梢血CD4+細胞数は、非投与群と比較して、投与群で4週齢時に有意に高値を示した。黒毛和種仔ウシにおいて、βカロテンの経口投与によって血清βカロテン濃度が上昇することで、末梢血の免疫担当細胞数が変化すると考えられ、βカロテン投与は末梢血白血球組成に影響を及ぼす可能性が示唆された。

  • Bacterial Isolation and Antimicrobial Susceptibility from Bronchoalveolar Lavage Fluids of Calves with Severe Pneumonia

    KURAMAE Tetsuro, ISHIKAWA Shingo, HAYASHI Jun, TSUMAGARI Keita, OTOMARU Konosuke, HOBO Seiji

    Journal of the Japan Veterinary Medical Association   73 ( 1 )   31 - 36   2020.01( ISSN:0446-6454

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    In this study, nasopharyngeal swabs (Swab) and bronchoalveolar lavage fluids (BALF) were collected from 50 Japanese black cattle diagnosed with severe chronic pneumonia, and identification of the causative bacteria of pneumonia and its antibiotic susceptibilities were investigated. <i>Mycoplasma bovis</i> and <i>Pasteurella multocida</i> were mainly isolated from Swab and BALF. However, the rates at which the same bacterial species were isolated from both specimens in the same cow were relatively low. In addition, <i>M. bovis</i> and <i>P. multocida</i> isolated from BALF were mainly sensitive to fluoroquinolones, but the antibiotic susceptibilities of the same two species isolated from Swab were low. From the above, <i>M. bovis</i> and <i>P. multocida</i> were mainly isolated from the nasopharyngeal area and bronchoalveolar area of cattle with severe chronic pneumonia, but the antibiotic susceptibility differs depending on the collection site, even if the same bacterial species is a possibility. It seems necessary to be careful about estimating the pneumonia causing bacteria by Swab in calves with severe pneumonia.

    DOI: 10.12935/jvma.73.31

    CiNii Article

    Other URL: http://id.ndl.go.jp/bib/030169943

  • 重症慢性肺炎罹患牛の気管支肺胞洗浄液からの細菌分離と薬剤感受性

    藏前 哲郎, 石川 真悟, 林 淳, 津曲 圭太, 乙丸 孝之介, 帆保 誠二

    日本獣医師会雑誌   73 ( 1 )   31 - 36   2020.01( ISSN:0446-6454

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    本研究では、臨床的に重症慢性肺炎と診断された黒毛和種牛50頭から鼻咽頭スワブ(Swab)及び気管支肺胞洗浄液(BALF)を採取し、細菌分離とともに、その薬剤感受性を調査した。Swab及びBALFからはおもにMycoplasma bovis及びPasteurella multocidaが分離されたが、同一牛において両検体から同一細菌種が分離された割合は比較的低かった。また、BALFから分離されたM.bovis及びP.multocidaは、おもにフルオロキノロン系抗菌薬に感受性であったが、Swabから分離された同2菌種の同系抗菌薬に対する薬剤感受性は低かった。以上より、重症慢性肺炎罹患牛の鼻咽頭領域及び気管支肺胞領域からは、おもにM.bovis及びP.multocidaが分離されるが、同一供試牛から分離された同一菌種の細菌であっても、薬剤感受性が異なる可能性があることから、重症慢性肺炎罹患牛においてSwabによる肺炎原因菌の推定には慎重を要すると思われた。(著者抄録)

  • Effect of Beta-Carotene Supplementation on the Peripheral Blood Leukocyte Population in Japanese Black Calves.

    Konosuke Otomaru, Rei Ogawa, Shoko Oishi, Yuki Iwamoto, Shingo Ishikawa, Katsuhisa Nagai

    Journal of nutritional science and vitaminology   66 ( 4 )   381 - 385   2020( ISSN:0301-4800

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:Domestic journal  

    The purpose of this study was to determine the effect of beta-carotene supplementation to Japanese Black calves on the peripheral blood leukocyte population. Twenty-two Japanese Black calves were alternately assigned to two groups. Eleven calves received 20 mg/d of beta-carotene orally from 2 to 8 wk of age (BC group), and the other 11 calves did not receive (control group). The serum beta-carotene concentration in the BC group at 4, 8 and 12 wk of age were significantly higher than those in the control group (p<0.05). The numbers of CD4+ cells in the BC group were significantly higher than those in the control group at 4 wk of age (p<0.05). These results confirmed that beta-carotene supplementation to Japanese Black calves affected the peripheral blood leukocyte population.

    DOI: 10.3177/jnsv.66.381

    PubMed

  • Distribution of marbofloxacin in the bronchoalveolar region in healthy calves.

    Jun Hayashi, Konosuke Otomaru, Masaya Hirata, Shingo Ishikawa, Tomonobu Ikedo, Chie Horinouchi, Tetsurou Kuramae, Keita Tsumagari, Seiji Hobo

    The Journal of veterinary medical science   81 ( 5 )   730 - 733   2019.05( ISSN:0916-7250

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:Domestic journal  

    The purpose of this study was to clarify the distribution of marbofloxacin (MBFX) within the bronchoalveolar region of calves. Four clinically healthy calves were intramuscularly injected with a single dose of MBFX (2 mg/kg). Samples of plasma and bronchoalveolar lavage fluid (BALF) were obtained for each calf at 0 (before administration), 1, 2, 6 and 24 hr after injection of MBFX. The injections and series of sample collections were conducted and repeated again after two weeks. The results show that the MBFX concentrations in the pulmonary epithelial lining fluid (ELF) were significantly higher than that in plasma and in alveolar cells at 2 hr after injection (P<0.05). For concentrations of MBFX within the ELF, the mean area under the MBFX concentration curve calculated during the 0 to 24 hr timeframe (AUC0-24) was significantly higher than the mean determined from samples collected from the plasma (P<0.05). These results suggest that intramuscularly injected MBFX was well distributed in the bronchoalveolar region.

    DOI: 10.1292/jvms.18-0761

    PubMed

  • 健康な仔牛の気管支肺胞領域におけるマルボフロキサシンの分布(Distribution of marbofloxacin in the bronchoalveolar region in healthy calves)

    Hayashi Jun, Otomaru Konosuke, Hirata Masaya, Ishikawa Shingo, Ikedo Tomonobu, Horinouchi Chie, Kuramae Tetsurou, Tsumagari Keita, Hobo Seiji

    The Journal of Veterinary Medical Science   81 ( 5 )   730 - 733   2019.05( ISSN:0916-7250

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MISC

  • 【牛の免疫のすすめ】呼吸器の免疫

    石川 真悟

    臨床獣医   41 ( 8 )   26 - 31   2023.07( ISSN:0912-1501

  • 自然免疫を活用した畜産現場における感染症予防の未来

    石川 真悟

    家畜感染症学会誌   12 ( 2 )   43 - 47   2023.05( ISSN:2186-7208

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    家畜の感染症予防のために「免疫力を上げて強い家畜を作る」という考え方が古くから提唱されてきた。家畜感染症における「免疫力」とは抗病性と言い換えることができ、「免疫力を上げる」は、免疫を調節して抗病性を高める、という考え方であると言える。家畜ではそのための手段として、獲得免疫の記憶機構を利用して、特異的抗原に対する免疫応答を調整するワクチンが広く用いられてきた。そのため、新規ワクチンの開発やワクチンの効果を高めるための飼養管理方法の検討等、免疫による感染症予防の焦点は獲得免疫応答にあてられ研究が進められてきた。近年、記憶が存在しないと考えられていた自然免疫においても、病原体が有する特有の分子パターン(病原体関連分子パターン:PAMPs)等を自然免疫受容体(パターン認識受容体)が認識することで記憶のような状態が発達する場合があり、抗原の種類にとらわれずに自然免疫の反応性が高まる「自然免疫記憶」という概念が提唱され、自然免疫の調整が「免疫力を上げる」ための手段として注目を集めている。「自然免疫記憶」に関する多くの知見はヒトやげっ歯類でもたらされているものであるが、家畜においてもこの概念が提唱される前から、現在はPAMPsとして認識されている物質を活用した感染症予防対策が検討されており、近年では自然免疫をターゲットとした予防法の開発も進んでいる。本稿では、ヒトやげっ歯類の知見から得られている「自然免疫記憶」の概念および家畜における自然免疫と感染症に関する研究について紹介し、自然免疫を活用した畜産現場における感染症予防の未来について考えたい。(著者抄録)

  • 黒毛和種哺乳子牛における胸囲充足率と呼吸器病との関連性

    叶 有斗, 福山 悠介, 石川 真悟

    家畜診療   69 ( 6 )   357 - 357   2022.06( ISSN:0287-0754

  • 牛肺炎診断への気管支肺胞洗浄の応用

    帆保誠二, 石川真悟

    MPアグロジャーナル   39   20 - 23   2019.10

  • 黒毛和種子牛における牛RSウイルス病の早期診断と病態および対策

    叶 有斗, 石川 真悟

    家畜診療   66 ( 5 )   306 - 307   2019.05( ISSN:0287-0754

  • 牛肺炎に対する気管支肺胞洗浄の応用と抗菌薬療法ならびに予防法 真の肺炎原因菌の特定と肺炎の治療及び予防

    帆保 誠二, 石川 真悟

    家畜診療   66 ( 3 )   153 - 163   2019.03( ISSN:0287-0754

  • 牛肺炎に対する気管支肺胞洗浄の応用と抗菌薬療法ならびに予防法 真の肺炎原因菌の特定と肺炎の治療及び予防

    帆保 誠二, 石川 真悟

    家畜診療   66 ( 3 )   153 - 163   2019.03( ISSN:0287-0754

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Charge of on-campus class subject

  • Practical Training for Food  production

    2021   Practical Training  

  • Practice for Large Animal Clinics

    2021   Practical Training  

  • Field Practice for Large Animal Clinics

    2021   Practical Training  

  • Stock Farm Practice

    2021   Practical Training  

  • Large Animal Practice B

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  • Diagnosis and Treatment in Large Animals B

    2021    

  • Veterinary Clinical Nutrition

    2021    

  • Basic clinical practices

    2021   Practical Training  

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  • 臨床基礎実習(分担)

    2021.04
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  • 大動物臨床B

    2021.04
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    Now

  • 大動物診断治療学B

    2021.04
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    Now

  • 食料生産実習(分担)

    2021.03
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  • 獣医臨床基礎実習(分担)(鹿児島大学)

    2018.04
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    2021.03

  • 臨床獣医学特別実習VIII(分担)(鹿児島大学)

    2017.04
    -
    2021.03

  • 牛診療学I(分担)(鹿児島大学)

    2016.04
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    2021.03

  • 動物感染症学A(分担)(鹿児島大学)

    2016.04
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    2021.03

  • 動物の病気(分担)(鹿児島大学)

    2016.04
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    2021.03

  • 産業動物診断治療学実習(分担)(鹿児島大学)

    2015.04
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    2021.03

  • 産業動物 特別演習(分担)(鹿児島大学)

    2015.04
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    2021.03

  • 産業動物総合臨床実習(分担)(鹿児島大学)

    2015.04
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    2021.03

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