Updated on 2024/03/25

写真a

 
SASAKI NOBUHIRO
 
Organization
Graduate School of Agriculture Department of Agricultural Biology Professor
School of Agriculture Department of Agricultural Biology
Title
Professor
Affiliation
Institute of Agriculture
Affiliation campus
Nakamozu Campus

Position

  • Graduate School of Agriculture Department of Agricultural Biology 

    Professor  2022.04 - Now

  • School of Agriculture Department of Agricultural Biology 

    Professor  2022.04 - Now

Degree

  • 博士(工学) ( Tokyo University of Agriculture and Technology )

Research Areas

  • Life Science / Plant molecular biology and physiology

Research Interests

  • betalain

  • anthocyanin

  • Plant pigment

Professional Memberships

  • 日本植物生理学会

    2004.01 - Now

  • 日本植物バイオテクノロジー学会

    2004.01 - Now

  • 日本植物学会

    1999.01 - Now

  • 日本植物細胞分子生物学会

  • 日本植物生理学会

  • 日本植物学会

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Committee Memberships (off-campus)

  • 医薬品の成分本質に関するワーキンググループ   厚生労働省  

    2022.05 - 2023.03 

  • 食品安全委員会専門委員   内閣府食品安全委員会  

    2022.04 - 2023.03 

Awards

  • 奨励賞

    2016.09   日本植物細胞分子生物学会  

  • 論文賞

    穐山浩、佐々木伸大、大木果林、中村文美、坂田こずえ、中村公亮、大森清美、中島安基江、古井聡、橘田和美、小関良宏、手島玲子

    2010.06   日本食品化学学会   PCR法を用いた米加工品の安全性未審査遺伝子組換え米の検知法

  • 学生奨励賞

    2005.08   日本植物細胞分子生物学会  

Job Career (off-campus)

  • 大阪公立大学   大学院農学研究科応用生物科学専攻

    2022.04 - Now

  • Toyo University

    2019.04 - 2022.03

  • Toyo University

    2017.04 - 2019.03

Papers

  • Production of yellow-flowered gentian plants by genetic engineering of betaxanthin pigments.

    Masahiro Nishihara, Akiko Hirabuchi, Fumina Goto, Yuzo Nishizaki, Shota Uesugi, Aiko Watanabe, Keisuke Tasaki, Rie Washiashi, Nobuhiro Sasaki

    The New phytologist   240 ( 3 )   1177 - 1188   2023.08( ISSN:0028646X

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Genetic engineering of flower color provides biotechnological products such as blue carnations or roses by accumulating delphinidin-based anthocyanins not naturally existing in these plant species. Betalains are another class of pigments that in plants are only synthesized in the order Caryophyllales. Although they have been engineered in several plant species, especially red-violet betacyanins, the yellow betaxanthins have yet to be engineered in ornamental plants. We attempted to produce yellow-flowered gentians by genetic engineering of betaxanthin pigments. First, white-flowered gentian lines were produced by knocking out the dihydroflavonol 4-reductase (DFR) gene using CRISPR/Cas9-mediated genome editing. Beta vulgaris BvCYP76AD6 and Mirabilis jalapa MjDOD, driven by gentian petal-specific promoters, flavonoid 3',5'-hydroxylase (F3'5'H) and anthocyanin 5,3'-aromatic acyltransferase (AT), respectively, were transformed into the above DFR-knockout white-flowered line; the resultant gentian plants had vivid yellow flowers. Expression analysis and pigment analysis revealed petal-specific expression and accumulation of seven known betaxanthins in their petals to c. 0.06-0.08 μmol g FW-1 . Genetic engineering of vivid yellow-flowered plants can be achieved by combining genome editing and a suitable expression of betaxanthin-biosynthetic genes in ornamental plants.

    DOI: 10.1111/nph.19218

    PubMed

  • Identification of Candidate Genes Responsible for Flower Colour Intensity in Gentiana triflora

    Keisuke Tasaki, Aiko Watanabe, Keiichirou Nemoto, Shigekazu Takahashi, Fumina Goto, Nobuhiro Sasaki, Takashi Hikage, Masahiro Nishihara

    Frontiers in Plant Science   13   2022.06

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    Publishing type:Research paper (scientific journal)  

    Gentians cultivated in Japan (Gentiana triflora and Gentiana scabra and hybrids) have blue flowers, but flower colour intensity differs among cultivars. The molecular mechanism underlying the variation in flower colour intensity is unclear. Here, we produced F<sub>2</sub> progeny derived from an F<sub>1</sub> cross of intense- and faint-blue lines and attempted to identify the genes responsible for flower colour intensity using RNA-sequencing analyses. Comparative analysis of flower colour intensity and transcriptome data revealed differentially expressed genes (DEGs), although known flavonoid biosynthesis-related genes showed similar expression patterns. From quantitative RT-PCR (qRT-PCR) analysis, we identified two and four genes with significantly different expression levels in the intense- and faint-blue flower lines, respectively. We conducted further analyses on one of the DEGs, termed GtMIF1, which encodes a putative mini zinc-finger protein homolog, which was most differently expressed in faint-blue individuals. Functional analysis of GtMIF1 was performed by producing stable tobacco transformants. GtMIF1-overexpressing tobacco plants showed reduced flower colour intensity compared with untransformed control plants. DNA-marker analysis also confirmed that the GtMIF1 allele of the faint-blue flower line correlated well with faint flower colour in F<sub>2</sub> progeny. These results suggest that GtMIF1 is one of the key genes involved in determining the flower colour intensity of gentian.

    DOI: 10.3389/fpls.2022.906879

  • Identification and characterization of xanthone biosynthetic genes contributing to the vivid red coloration of red-flowered gentian.

    Nobuhiro Sasaki, Keiichirou Nemoto, Yuzo Nishizaki, Naoki Sugimoto, Keisuke Tasaki, Aiko Watanabe, Fumina Goto, Atsumi Higuchi, Ed Morgan, Takashi Hikage, Masahiro Nishihara

    The Plant journal : for cell and molecular biology   107 ( 6 )   1711 - 1723   2021.09( ISSN:0960-7412

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Cultivated Japanese gentians traditionally produce vivid blue flowers because of the accumulation of delphinidin-based polyacylated anthocyanins. However, recent breeding programs developed several red-flowered cultivars, but the underlying mechanism for this red coloration was unknown. Thus, we characterized the pigments responsible for the red coloration in these cultivars. A high-performance liquid chromatography with photodiode array analysis revealed the presence of phenolic compounds, including flavones and xanthones, as well as the accumulation of colored cyanidin-based anthocyanins. The chemical structures of two xanthone compounds contributing to the coloration of red-flowered gentian petals were determined by mass spectrometry and nuclear magnetic resonance spectroscopy. The compounds were identified as norathyriol 6-O-glucoside (i.e., tripteroside designated as Xt1) and a previously unreported norathyriol-6-O-(6'-O-malonyl)-glucoside (designated Xt2). The copigmentation effects of these compounds on cyanidin 3-O-glucoside were detected in vitro. Additionally, an RNA sequencing analysis was performed to identify the cDNAs encoding the enzymes involved in the biosynthesis of these xanthones. Recombinant proteins encoded by the candidate genes were produced in a wheat germ cell-free protein expression system and assayed. We determined that a UDP-glucose-dependent glucosyltransferase (StrGT9) catalyzes the transfer of a glucose moiety to norathyriol, a xanthone aglycone, to produce Xt1, which is converted to Xt2 by a malonyltransferase (StrAT2). An analysis of the progeny lines suggested that the accumulation of Xt2 contributes to the vivid red coloration of gentian flowers. Our data indicate that StrGT9 and StrAT2 help mediate xanthone biosynthesis and contribute to the coloration of red-flowered gentians via copigmentation effects.

    DOI: 10.1111/tpj.15412

    PubMed

  • Identification of the Biosynthetic Pathway for Anthocyanin Triglucoside, the Precursor of Polyacylated Anthocyanin, in Red Cabbage.

    Riko Horiuchi, Yuzo Nishizaki, Natsumi Okawa, Ayaka Ogino, Nobuhiro Sasaki

    Journal of agricultural and food chemistry   68 ( 36 )   9750 - 9758   2020.09( ISSN:0021-8561

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    Publishing type:Research paper (scientific journal)   International / domestic magazine:International journal  

    Red cabbage anthocyanin is utilized as a natural food colorant because of its stable and brilliant coloration. The major anthocyanin of red cabbage is cyanidin (Cy) mono- and di-acyltriglucoside; however, the biosynthetic pathway to generate this anthocyanin remains unclear. We isolated and identified four uridine diphosphate-glucose-dependent glucosyltransferase (UGT) cDNAs from red cabbage using RNA-seq. UGTs are involved in Cy triglucoside (CytriG) synthesis, the precursor of Cy acyltriglucoside. Enzymatic assays using recombinant proteins suggested that UGT78D5 encodes Cy 3GT, UGT79B45 encodes Cy 3-glucoside GT, UGT75C2 encodes Cy 3-sophoroside (Cy3Sp) 5GT, and UGT79B44 encodes flavonol 3-glucoside GT. Anthocyanin GT assays using crude proteins prepared from red cabbage suggested that CytriG is produced from intermediate products in the following order: Cy, Cy3G, Cy3Sp, and CytriG.

    DOI: 10.1021/acs.jafc.0c03480

    PubMed

  • Effects of knocking out three anthocyanin modification genes on the blue pigmentation of gentian flowers. Reviewed

    Keisuke Tasaki, Atsumi Higuchi, Aiko Watanabe, Nobuhiro Sasaki, Masahiro Nishihara

    Scientific reports   9 ( 1 )   15831 - 15831   2019.11

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    International / domestic magazine:International journal  

    Genome editing by the CRISPR/Cas9 system has recently been used to produce gene knockout lines in many plant species. We applied this system to analyze Japanese gentian plants that produce blue flowers because of the accumulation of a polyacylated anthocyanin, gentiodelphin. Mutant lines in which anthocyanin modification genes were knocked out were examined to assess the contribution of each gene to the blue pigmentation of flowers. The targeted genes encoded anthocyanin 5-O-glycosyltransferase (Gt5GT), anthocyanin 3'-O-glycosyltransferase (Gt3'GT), and anthocyanin 5/3'-aromatic acyltransferase (Gt5/3'AT). The Gt5GT knockout lines accumulated delphinidin 3G, whereas the Gt3'GT knockout lines accumulated delphinidin 3G-5CafG as the major flower pigment. Knocking out Gt5/3'AT resulted in the accumulation of delphinidin 3G-5G-3'G and delphinidin 3G-5G as the primary and secondary pigments, respectively. These results indicated the existence of two pathways mediating the modification of delphinidin 3G-5G in flowers, with one involving a glycosylation by 3'GT and the other involving an acylation by 5/3'AT. The Gt5GT, Gt3'GT, and Gt5/3'AT transformants produced pale red violet, dull pink, and pale mauve flowers, respectively, unlike the vivid blue flowers of wild-type plants. Thus, the glycosylation and subsequent acylation of the 3'-hydroxy group of the B-ring in delphinidin aglycone is essential for the development of blue gentian flowers.

    DOI: 10.1038/s41598-019-51808-3

    PubMed

MISC

  • Molecular analysis of red flower color development in red-flowered gentian cultivars

    NEMOTO Keiichirou, SASAKI Nobuhiro, SASAKI Nobuhiro, NISHIZAKI Yuzo, SUGIMOTO Naoki, TASAKI Keisuke, TASAKI Keisuke, WATANABE Aiko, GOTO Fumina, HIGUCHI Atsumi, MORGAN Ed, HIKAGE Takashi, NISHIHARA Masahiro

    日本植物生理学会年会(Web)   61st   2020

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  • ゲノム編集技術のリンドウへの適用

    西原昌宏, 渡辺藍子, 後藤史奈, 吉田千春, 根本圭一郎, 高橋重一, 佐々木伸大, 佐々木伸大, 高橋秀行, 高橋秀行, 田崎啓介, 田崎啓介

    日本植物細胞分子生物学会大会・シンポジウム講演要旨集   37th   2019

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Presentations

  • 紫キャベツにおけるアントシアニンアシル化酵素遺伝子の探索 Domestic conference

    本田佳留奈, 西﨑雄三, 佐々木伸大

    第40回日本植物バイオテクノロジー学会(千葉)大会  2023.09  日本植物バイオテクノロジー学会

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    Venue:千葉  

  • 赤花リンドウの鮮赤色化に関わるキサントン類とその合成に関わる遺伝子の単離 Domestic conference

    植物色素研究会第 32 回集会  2022.11 

  • 植物色素生合成経路に関する研究 Domestic conference

    第18回けいはんな地区植物科学懇話会  2022.11 

  • 紫キャベツにおけるアントシアニンアシル基転移酵素遺伝子の探索

    本田佳留奈、西﨑雄三、佐々木伸大

    植物色素研究会 第 33 回大会 東京・ 2023(共催:植物色素談話会)   植物色素研究会・植物色素談話会

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    Venue:東京  

Outline of education staff

  • 遺伝子組換え食品や食品添加物等食品の安全性がどのように評価され担保されているかについて法令上の扱いも含めて教育をおこなっている。また、遺伝子組換え食品やゲノム編集食品がどのように作出され流通しているかについても原理を含めた講義を行っている。

Charge of on-campus class subject

  • 食料安全科学特論

    2023   Weekly class   Graduate school

  • バイオエコノミー論

    2023   Weekly class   Undergraduate

  • 国際食料流通演習

    2023   Intensive lecture   Undergraduate

  • 食料生産実習

    2023   Intensive lecture   Undergraduate

  • 初年次ゼミナール

    2023   Weekly class   Graduate school

  • 食料生産実習

    2023   Intensive lecture   Undergraduate

  • 植物バイオサイエンス卒業研究

    2023   Intensive lecture   Undergraduate

  • 食料安全科学

    2023   Weekly class   Undergraduate

  • 植物バイオサイエンス演習

    2023   Intensive lecture   Undergraduate

  • 植物科学英語

    2023   Intensive lecture   Undergraduate

  • 食料安全科学特論

    2022   Weekly class   Graduate school

  • 応用生物科学特論

    2022   Weekly class   Graduate school

  • 食料安全科学

    2022   Weekly class   Undergraduate

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