掲載種別：研究論文（学術雑誌）  

DOI： 10.3390/vetsci10010004

掲載種別：研究論文（学術雑誌）   国際・国内誌：国際誌  

The transcription factor hypoxia-inducible factor 1α (HIF-1α) is expressed in several cancers under intratumoral hypoxic stress that arises during pathogenic processes, resulting in malignant progression. We previously reported that hypoxic stimulation enhances the growth potential of canine lymphoma cells by activating the HIF-1α signaling pathway. In contrast, evofosfamide (Evo) releases a DNA-alkylating moiety within hypoxic tumor regions, suggesting that Evo could serve as a hypoxia-targeting drug in canine lymphoma. This study aimed to use Evo to evaluate hypoxia-targeted therapy in dogs with gastrointestinal lymphoma (GIL) and investigate how Evo affects antitumor efficacy and adverse events in three type of murine xenograft models using T-cell GIL cells. In vitro tests, the sensitivity to Evo of three T-cell GIL cell lines under hypoxic culture was significantly higher than that under normoxic culture. Our metabolic analysis suggested that the three murine models might have high reproducibility as clinical cases in canine GIL. Our data showed that Evo showed significantly higher tumor growth potential and fewer adverse events in three type of murine models compared to lomustine; CeeNu (CCNU). Additionally, Evo suppressed the expression of HIF-1α protein in tumor tissues, suggesting that it may preferentially target and inhibit tumor cells in a hypoxic region. The evidence presented here supports the favorable preclinical evaluation that Evo may be effective for GIL in dogs.

DOI： 10.1016/j.rvsc.2022.11.003

PubMed

掲載種別：研究論文（学術雑誌）   国際・国内誌：国内誌  

A 5-month-old intact female mixed cat presented with repetitive paraplegia and drainage of pus from the back despite continuous antibiotic medication. Neurologic examination was consistent with below T3-L3 myelopathy. Computed tomography and magnetic resonance imaging revealed a contrast-enhanced mass in the L1-3 spinal canal, and bone fragments in the T13 and L1 spinal canal. Spinal epidural empyema was suspected, and hemilaminectomy was performed for T12-L2 on the right side and T11-12 on the left side. Bone fragments were diagnosed as sequestrum infected with Bacteroides sp. The cat recovered enough to ambulate next day. One month after surgery, there was no deficit in neurological function. This is the first report of spinal epidural empyema concurrent with sequestrum in a cat.

DOI： 10.1292/jvms.22-0264

PubMed

担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）  

DOI： 10.1002/vms3.930

その他URL： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/vms3.930

掲載種別：研究論文（学術雑誌）  

The transcription factor hypoxia-inducible factor 1 alpha (HIF-1 alpha) is activated in response to oxygen deficiency, and is expressed in several cancers under intratumoral hypoxic stress that arises during pathogenic processes. Hypoxic stimulation enhanced the growth potential of canine lymphoma cells by activating the HIF-1 alpha signalling pathway in a previously reported study. The aim of this study was to establish a molecular design strategy for a novel hypoxia-targeting therapy for intestinal T-cell lymphoma (ITL) in dogs. We assessed the relationship between immunohistochemistry-based HIF-1 alpha expression and clinical information, including signalment, tumour area, clinical signs, systemic diseases, treatment protocol, follow-up information, chemotherapy response and overall survivals (OS), using 48 tissue samples from dogs with ITL. We investigated the effects of hypoxic stimulation on the biological behaviour of cell lines from three different types of canine ITL. We assessed the effects of evofosfamide (Evo; hypoxia-activated prodrug) on cell lines cultured under hypoxic conditions. Our data showed that treatment response and overall survival might be significantly decreased in dogs with higher HIF-1 alpha expression than in those with lower HIF-1 alpha expression. Hypoxic culture (1% O-2, 72 h) enhanced the invasiveness of cell lines and decreased their sensitivity to CCNU, resulting in hypoxia-dependent aggressive behaviour. Sensitivity to Evo significantly increased in cell lines cultured under hypoxia compared with those cultured under normoxia, which exhibited hypoxia-dependent apoptosis. Additionally, Evo downregulated HIF-1 alpha expression in cell lines cultured under hypoxia, suggesting that Evo might inhibit cell growth by inactivating HIF-1 alpha-dependent cell signalling. Our results revealed the preclinical antitumor activity of Evo and provide a rationale for treatment strategies for dogs with ITL.

DOI： 10.1111/vco.12855

担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）  

DOI： 10.1002/vrc2.420

その他URL： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/vrc2.420

掲載種別：研究論文（学術雑誌）  

DOI： 10.3389/fvets.2022.845809

掲載種別：研究論文（学術雑誌）   国際・国内誌：国際誌  

Published studies describing outcomes for cats with nasal lymphoma (NLSA) receiving first-line palliative radiation (PRT) versus PRT after chemotherapy failure are currently lacking. The aims of this retrospective observational study were to compare outcomes for cats with NLSA that were treated with these two methods. A total of 48 cats were included in analyses; 32 receiving PRT alone and 16 receiving PRT after chemotherapy failure. The treatment response, progression-free survival (PFS), disease-specific survival (DSS), overall survival (OS), and incidence rate of systemic disease were compared between the two groups. The overall response rate (ORR) was calculated from the same target lesions between pre-RT (within a week before starting PRT) and post-RT (on date of PRT completion) by computed tomography (CT) imaging. The ORR was 94% in cats that received PRT alone, 13 had a complete response (CR) and 17 had a partial response (PR). The ORR was 88% in cats that received PRT after chemotherapy failure, with five having CR and nine with PR. There were no significant differences in the ORR between the two groups. The PFS, DSS, and OS significantly increased in the cats that received PRT alone compared to the cats that received PRT after chemotherapy failure (median PFS: 336 vs 228 days, P = 0.0012, median DSS: 360 vs 242 days, P = 0.0025, median OS: 346 vs 242 days, P = 0.0036, respectively). The incidence rate of systemic disease significantly increased in 75% (12/16) of cats receiving PRT after chemotherapy failure compared to 41% (13/32) of cats receiving PRT alone. The results suggested that clinical outcomes may improve in cats with NLSA with first-line PRT compared to PRT after chemotherapy failure.

DOI： 10.1111/vru.13075

PubMed

担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）  

DOI： 10.1002/vrc2.303

掲載種別：研究論文（学術雑誌）  

DOI： 10.1002/vms3.840

その他URL： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/vms3.840

担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）   国際・国内誌：国際誌  

DOI： 10.1002/vetr.1083

PubMed

担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）   国際・国内誌：国際誌  

DOI： 10.1177/1098612X211046847

PubMed

担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）  

DOI： 10.1002/vms3.598

掲載種別：研究論文（学術雑誌）   国際・国内誌：国内誌  

DOI： 10.1292/jvms.20-0656

PubMed

掲載種別：研究論文（学術雑誌）   国際・国内誌：国際誌  

DOI： 10.1111/vco.12653

PubMed

担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）  

DOI： 10.14943/jjvr.69.3.163

担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）   国際・国内誌：国際誌  

DOI： 10.1136/vetreco-2019-000371

PubMed

掲載種別：研究論文（学術雑誌）   国際・国内誌：国際誌  

DOI： 10.1111/jvim.15768

PubMed

担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）  

掲載種別：研究論文（学術雑誌）   国際・国内誌：国際誌  

OBJECTIVE: To measure expression of microRNAs (miRNAs) in plasma and in extracellular vesicles (EVs) derived from plasma for dogs with glioma and dogs with other brain diseases. SAMPLE: Plasma samples from 11 dogs with glioma and 19 control dogs with various other brain diseases. PROCEDURES: EVs were isolated from plasma samples by means of ultracentrifugation. Expression of 4 candidate reference miRNAs (let-7a, miR-16, miR-26a, and miR-103) and 4 candidate target miRNAs (miR-15b, miR-21, miR-155, and miR-342-3p) was quantified with reverse transcription PCR assays. Three software programs were used to select the most suitable reference miRNAs from among the 4 candidate reference miRNAs. Expression of the 4 target miRNAs was then calculated relative to expression of the reference genes in plasma and EVs, and relative expression was compared between dogs with glioma and control dogs with other brain diseases. RESULTS: The most suitable reference miRNAs were miR-16 for plasma and let-7a for EVs. Relative expression of miR-15b in plasma and in EVs was significantly higher in dogs with glioma than in control dogs. Relative expression of miR-342-3p in EVs was significantly higher in dogs with glioma than in control dogs. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that miR-15b and miR-342-3p have potential as noninvasive biomarkers for differentiating glioma from other intracranial diseases in dogs. However, more extensive analysis of expression in specific glioma subtypes and grades, compared with expression in more defined control populations, will be necessary to assess their clinical relevance.

DOI： 10.2460/ajvr.81.4.355

PubMed

担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）   国際・国内誌：国際誌  

In dogs, diagnosis of prostate cancer is often delayed because clinical signs are not pathognomonic. Although ultrasonography is mainly performed to detect prostate cancer, the ultrasonographic appearance is not specific. In humans, magnetic resonance imaging (MRI), including diffusion-weighted imaging (DWI) is used to localize the prostate tumour. To our knowledge, there are no studies of MRI findings for detecting or localizing prostate cancer. The purpose of this study was to assess MRI findings of prostate cancer. As a control, three prostate hyperplasia cases were included. MRI data were analysed, and the following parameters were noted: signal intensity (SI) of prostate lesion on T2-weighted imaging, T1-weighted imaging and DWI, enhancement pattern of prostate lesion, and relative contrast enhancement indices (RCEI) and apparent diffusion coefficient (ADC) value of prostate lesion. For MRI examination, the mean RCEI of the adenocarcinoma was significantly lower than that of hyperplasia (P = .01, r = .8). The SI of the DWI of adenocarcinoma was significantly higher compared to hyperplasia (P = .03, φ = 1). The mean ADC values of the adenocarcinoma were significantly lower than that of hyperplasia (P = .03, r = .82). Thus, less enhancement on MRI, and low ADC value on MRI may help to detect prostate adenocarcinoma.

DOI： 10.1111/vco.12578

PubMed

掲載種別：研究論文（学術雑誌）   国際・国内誌：国内誌  

A cat was referred because of diffuse parenchymal lung disease. Close examinations revealed a swollen abdominal lymph node and multiple nodules of the liver. Mycobacterium avium subspecies hominissuis infection was confirmed by culture and single nucleotide polymorphism analysis of samples recovered from the liver and bronchoalveolar lavage. After administration of combination antibiotics for 6 months, culture results were negative. Though atonic seizures were observed during the treatment, it disappeared after isoniazid discontinuation and pyridoxal phosphate administration. On day 771 of illness, no clinical signs, lung diseases, or obvious swelling of lymph nodes was observed. This is the first report to confirm Mycobacterium avium subspecies hominissuis infection in cats through gene analysis and to completely cure it with combination antibiotics.

DOI： 10.1292/jvms.19-0492

PubMed

担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）   国際・国内誌：国際誌  

In veterinary medicine, abdominal ultrasonography is used to rank the differential diagnosis of renal lesions. However, a conventional sonographic examination may show nonspecific findings. The purpose of this study was to assess the computed tomography (CT) findings of canine renal tumors, including renal cell carcinoma (RCC), lymphoma, and hemangiosarcoma (HSA). In this retrospective study, the following CT parameters were recorded for each dog: 1) extent of renal involvement of tumors, 2) enhancement pattern, 3) number of renal tumors, 4) renal tumor vessel enhancement in the corticomedullary phase, 5) presence of lymphadenopathy and lung metastasis, and 6) attenuation values of the renal tumors on the pre- and post-contrast corticomedullary, nephrographic, and excretory phase images. Fifteen dogs met the inclusion criteria, of which nine had RCCs, four had lymphomas, and two had HSAs. RCCs tended to show heterogeneous enhancement and unilateral renal involvement, and vessel enhancement was detected in the corticomedullary phase in dogs with RCC. Conversely, renal lymphomas showed homogeneous enhancement, bilateral renal involvement, and multiple masses; in these dogs, no vessel enhancement was detected in the corticomedullary phase, and the incidence of lymphadenopathy was low. However, in dogs with lymphadenopathy, the renal lymphoma was associated with regionally severe lymphadenopathy. Finally, renal HSAs tended to show heterogeneous enhancement with a non-enhanced area and unilateral renal involvement; in these dogs, vessel enhancement was detected in the nephrographic phase, with the enhancement expanding around the vessel. These findings had no significant differences. Further studies with a larger sample size are required to examine the association between CT and histopathological findings.

DOI： 10.1371/journal.pone.0225211

PubMed

担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）  

担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）   国際・国内誌：国際誌  

DOI： 10.1177/1098612X17738615

PubMed

担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）  

担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）   国際・国内誌：国際誌  

DOI： 10.1177/2055116917750762

PubMed

担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）   国際・国内誌：国際誌  

DOI： 10.1111/ahe.12390

PubMed

掲載種別：研究論文（学術雑誌）  

<p>12歳3カ月，雄，体重5.2kgのミニチュア・ダックスフンドが肝臓の腫瘤を主訴に来院した．症例は膵炎の既往歴を有していた．初診時，症例は元気消失を呈していた．血液学的検査では白血球数の増加と血小板数の減少を認め，血液生化学的検査ではC反応性タンパク質（CRP）濃度の上昇を認めた．血液凝固系検査では特記すべき所見を認めなかった．超音波検査では，モザイク状に低エコー源性を示す2つの脾臓腫瘤及び肝臓実質と同程度のエコー源性を示す肝臓腫瘤を認めた．第2病日に脾臓の全摘出及び肝臓腫瘤の切除を実施した．脾臓の2つの腫瘤は膿瘍であり，膿汁の細菌培養により大腸菌が検出された．肝臓腫瘤は病理組織学的に結節性過形成と診断された．本症例では，膵炎が基礎疾患として脾膿瘍形成の一因となったと推測された．</p>

DOI： 10.12935/jvma.70.114

CiNii Article

その他URL： https://agriknowledge.affrc.go.jp/RN/2010910497

担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）  

DOI： 10.1136/vr.101238

J-GLOBAL

担当区分：筆頭著者  

The aim of this study was to evaluate MHC class I expression and prognosis using tumor tissues surgically removed from 9 dogs with mammary gland carcinomas and from 13 dogs with complex carcinomas. We assessed MHC class I expression and its correlation with tumor size, B2M expression, infiltration of lymphocytes, histological grade and prognosis. Hematoxylin and eosin-stained sections were histologically graded using the Elston and Ellis grading method. MHC class I expression on tumor cells was evaluated using the avidinbiotin peroxidase complex method. Loss of MHC class I expression from canine mammary gland carcinomas was significantly correlated with poor prognosis (P&lt
0.05). Loss of MHC class I expression showed no association with poor prognosis in canine mammary gland complex carcinomas, because the data were not balanced. Only 1 of 13 (7.6%) canine mammary gland complex carcinomas showed loss of MHC class I expression. All 13 of these dogs showed good prognosis. Thus, the low frequency of MHC class I expression loss from canine mammary gland complex carcinomas may be associated with good prognosis. Taken together, these results suggest that loss of MHC class I expression may be associated with poor prognosis in canine mammary gland carcinomas. © 2013 The Japanese Society of Veterinary Science.

DOI： 10.1292/jvms.13-0080

PubMed

J-GLOBAL

掲載種別：研究論文（学術雑誌）  

Pulmonary surfactant protein A (SP-A) is used as a biomarker to understand the clinical features of pulmonary diseases and associated prognostic indices in human medicine. This study was conducted to investigate whether or not serum SP-A concentration can be used as a biomarker for identifying pulmonary parenchymal diseases in dogs. Thirty-two dogs with pulmonary parenchymal diseases, 34 with nonrespiratory diseases and 57 healthy dogs were included. Serum SP-A concentration was measured in all dogs using sandwich enzyme linked immunosorbent assay with an anti-dog SP-A polyclonal antibody. Median serum SP-A concentration in healthy dogs was &lt
2.0 ng/ml, whereas that in dogs with aspiration pneumonia (n=11), primary lung tumors (n=9) and blunt traumatic lung injury (BTLI
n=12) was 3.1, 7.2 and 2.6 ng/ml, respectively
these values were significantly higher than those in healthy dogs. The serum SP-A concentration in dogs with nonrespiratory diseases was comparable with that in healthy dogs. No correlation was observed between the serum SP-A and plasma C-reactive protein concentrations in dogs with aspiration pneumonia and BTLI. There was a significant correlation between the serum SP-A concentration and thoracic radiographic changes in dogs with BTLI. These findings suggest that the serum SP-A concentration may be a useful clinical biomarker of alveolar damage that can be used for differential diagnosis of pulmonary parenchymal diseases and nonrespiratory diseases in dogs. © 2013 The Japanese Society of Veterinary Science.

DOI： 10.1292/jvms.12-0255

PubMed

J-GLOBAL

担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）  

A 5-month-old male Abyssinian presented with right hemiplegia. Cervical radiographs, haematology and serum biochemistry were normal. A foreign body was identified on computed tomography (CT) and magnetic resonance imaging (MRI). Removal of the foreign body was carried out via a dorsal approach between the occipital bone and the C1 vertebral body using intra-operative CT. No complications were encountered. Thirty days after surgery, the cat was ambulatory.

DOI： 10.1177/1098612X11429232

PubMed

J-GLOBAL

担当区分：筆頭著者  

CiNii Article

その他URL： http://search.jamas.or.jp/link/ui/2013041481

掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.vetimm.2011.08.024

J-GLOBAL

担当区分：筆頭著者  

未去勢雄，1歳9カ月齢のアメリカンコッカースパニエルが元気食欲低下，後肢のふらつきを主訴に来院した． 血液生化学検査ではALT，TP，Glb，Caの上昇，血球検査では軽度貧血，白血球数の低下が認められた． また，蛋白電気泳動では単クローン性高<i>γ</i>グロブリン血症が認められた． CT，MRI検査ならびに骨髄生検の結果より，多発性骨髄腫による骨融解とそれに伴う脊髄の硬膜外圧迫性病変と診断した． 本症例はシクロホスファミド，メルファランおよびプレドニゾロン（MP療法）の投与でも高蛋白血症，高Ca血症，貧血の改善は認められなかったが，人医療で用いられているビンクリスチン，ドキソルビシンおよびデキサメタゾンの投与（VAD療法）で早期に寛解が得られ，QOLは著しく向上した． しかし，第103病日，四肢麻痺，呼吸困難を呈し斃死した．

DOI： 10.12935/jvma.63.797

CiNii Article

J-GLOBAL

その他URL： https://agriknowledge.affrc.go.jp/RN/2010800281

担当区分：筆頭著者  

未避妊雌、10歳齢のゴールデンレトリーバーが元気消失、痙攣を主訴に来院した。血液検査では明らかな異常は認められず、MRI検査により前頭葉に腫瘤の存在が認められた。術中MRIを併用して経前頭洞開頭術により、腫瘤を切除した。切除した腫瘤は病理組織学的検査にて髄膜上皮型髄膜腫と診断された。腫瘍摘出後、再発が認められたものの、本症例は外科手術単独で707病日と長期生存した。<br>

DOI： 10.2327/jvas.41.29

CiNii Article

J-GLOBAL

その他URL： https://agriknowledge.affrc.go.jp/RN/2010812201

It is well known that the ultrasonically activated instrument is useful for general and laparoscopic surgery in human medicine. In veterinary medicine, there are few informations about the application of this instrument. This report introduced how to use the ultrasonically activated instrument, Harmonic Scalpel<SUP>&reg;</SUP> (Johnson & Johnson K.K.), for small animal surgical procedures. The report also showed some cases operated with the instrument.

DOI： 10.2327/jvas.34.71

CiNii Article

J-GLOBAL

担当区分：筆頭著者  

担当区分：筆頭著者   掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）  

担当区分：筆頭著者   掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）  

担当区分：筆頭著者   掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）  

J-GLOBAL

担当区分：筆頭著者   掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）  

担当区分：筆頭著者   掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）  

担当区分：筆頭著者  

担当区分：筆頭著者   掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）  

担当区分：筆頭著者   掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）  

担当区分：筆頭著者   掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）  

担当区分：筆頭著者   掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）  

J-GLOBAL

担当区分：筆頭著者  

担当区分：筆頭著者  

担当区分：筆頭著者  

担当区分：筆頭著者  

J-GLOBAL

担当区分：筆頭著者  

担当区分：筆頭著者  

担当区分：筆頭著者  

J-GLOBAL

J-GLOBAL

J-GLOBAL

J-GLOBAL

会議種別：公開講演，セミナー，チュートリアル，講習，講義等  

会議種別：口頭発表（一般）  

会議種別：口頭発表（一般）  

会議種別：口頭発表（一般）  

会議種別：口頭発表（一般）  

会議種別：口頭発表（一般）  

会議種別：口頭発表（一般）  

会議種別：口頭発表（一般）  

会議種別：口頭発表（一般）  

会議種別：口頭発表（招待・特別）  

会議種別：口頭発表（招待・特別）