2026/05/14 更新

写真a

アンドウ　ミツル
安藤　満
ANDO MITSURU
担当
大学院工学研究科 物質化学生命系専攻 講師
工学部 応用化学科
職名
講師
所属
工学研究院

担当・職階

  • 大学院工学研究科 物質化学生命系専攻 

    講師  2026年04月 - 継続中

  • 工学部 応用化学科 

    講師  2026年04月 - 継続中

取得学位

  • 博士（薬学） （ 京都大学 ）

職務経歴（学外）

  • 京都大学医生物学研究所   生命システム研究部門 老化感染制御学学分野   非常勤研究員

    2026年04月 - 継続中

  • 京都大学医生物学研究所   再生組織構築研究部門

    2022年04月 - 2026年03月

  • 京都大学 ウイルス・再生医科学研究所   再生組織構築研究部門

    2021年05月 - 2022年03月

  • 京都大学 ウイルス・再生医科学研究所   再生組織構築研究部門   教務補佐員

    2021年04月

  • 沖縄科学技術大学院大学   イメージングセクション   CryoEM trainee

    2021年02月

  • 沖縄科学技術大学院大学   イメージングセクション   CryoEM trainee

    2020年01月

  • JST-CREST [細胞外微粒子]   CREST博士研究員

    2018年04月 - 2020年03月

  • JST-ERATO秋吉バイオナノトランスポータープロジェクト   プロテオリポソーム工学 グループリーダー

    2017年04月 - 2018年03月

  • 京都大学   大学院工学研究科   特定研究員

    2014年04月 - 2021年03月

  • JST-ERATO秋吉バイオナノトランスポータープロジェクト   特定研究員

    2014年04月 - 2018年03月

  • 日本学術振興会   特別研究員(PD)

    2013年04月 - 2014年03月

  • 日本学術振興会   特別研究員(DC2)

    2012年04月 - 2013年03月

▼全件表示

論文

  • Preparation of Platelet Membrane‐Coated Gelatin Nanoparticle Toward Alternative to Platelet Preparation

    Mitsuru Ando, Keisuke Moriyama, Isamu Akiba, Yasuhiko Tabata

    Macromolecular Rapid Communications   2026年02月（ ISSN:1022-1336 ） （ eISSN:1521-3927 ）

    　詳細を見る

    担当区分：筆頭著者,　責任著者   掲載種別：研究論文（学術雑誌）  

    ABSTRACT

    Platelets play a critical role in hemostasis and tissue repair; however, their short shelf life of approximately four days poses a major challenge to their supply. This limitation underscores the need for long‐term storage alternatives. In this study, we developed and characterized platelet membrane–coated gelatin nanoparticles (PL‐GNPs) as potential platelet substitutes. PL‐GNPs were prepared by coating gelatin nanoparticles (GNPs) with platelet‐derived membrane vesicles. Cryo‐electron microscopy revealed the formation of a core–shell structure, and immunoblotting demonstrated that key platelet surface proteins, CD41 and CD61, were preserved on the PL‐GNP surface in the same orientation as in platelets. The membrane coating improved the stability of the nanoparticles against collagenase‐dependent degradation. Moreover, PL‐GNPs bound effectively to fibrinogen, an essential interaction in thrombus formation, and retained this function even after one month of storage at 4°C. Collectively, these findings indicate that PL‐GNPs recapitulate the critical structural and functional properties of native platelets and hold promise as a stable, long‐term storable alternative to conventional platelet preparations for therapeutic use.

    DOI： 10.1002/marc.202500826

    その他URL： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/marc.202500826

  • Poly(2‐Propyl‐2‐Oxazoline)‐Induced Lipid Nanotube Formation in Phospholipid Multilayers

    Ryosuke Mizuta, Tatsuhiko Murata, Mitsuru Ando, Tomoki Nishimura, Shin‐ichi Sawada, Kazunari Akiyoshi, Yoshihiro Sasaki

    Macromolecular Rapid Communications   2025年11月

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    掲載種別：研究論文（学術雑誌）  

    DOI： 10.1002/marc.202500702

  • Mesenchymal Stem Cell Membrane Coating of Gelatin Nanospheres with Drug Release Ability for Inflammatory Tissue Targeting

    Keisuke Moriyama, Mitsuru Ando, Kazuo Sakurai, Isamu Sakurai, Yasuhiko Tabata

    bioRxiv   2025年06月

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    担当区分：責任著者  

    DOI： 10.1101/2025.06.01.657318

  • Association with Cationized Gelatin Nanospheres Enhances Mitochondria Uptake and Membrane Potential.

    Wenxuan Yang, Satoshi Abe, Mitsuru Ando, Yasuhiko Tabata

    Tissue engineering. Part A   2024年12月

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    掲載種別：研究論文（学術雑誌）   国際・国内誌：国際誌  

    The objective of this study is to investigate the influence of exogenous mitochondria (Mt) internalization on the Mt membrane potential of cells. Cationized gelatin nanospheres (cGNS) were prepared to mix Mt at different ratios to prepare Mt associated with cGNS (Mt-cGNS). The Mt internalization depended on the Mt/cGNS mixing ratio to achieve the maximum at the ratio of 3/1. Rho 0 cells of a Mt function-deficient line were prepared to evaluate the enhancement of Mt membrane potential of rho 0 cells after the internalization of Mt-cGNS. When evaluated by using tetramethylrhodamine methyl ester reagent, the mitochondrial membrane potential of rho 0 cells after incubation with Mt-cGNS enhanced compared with that incubated with Mt only and maintained at a significantly higher level even for 6 days. The Mt-cGNS were internalized into rho 0 cells by an actin-dependent pathway, followed by fused with endogenous Mt. It is concluded that association with the cGNS enabled Mt to enhance the cellular internalization, followed by the fusion with endogenous Mt to maintain an enhanced Mt membrane potential.

    DOI： 10.1089/ten.tea.2024.0265

    PubMed

  • Distribution and Incorporation of Extracellular Vesicles into Chondrocytes and Synoviocytes.

    Takashi Ohtsuki, Ikumi Sato, Ren Takashita, Shintaro Kodama, Kentaro Ikemura, Gabriel Opoku, Shogo Watanabe, Takayuki Furumatsu, Hiroshi Yamada, Mitsuru Ando, Kazunari Akiyoshi, Keiichiro Nishida, Satoshi Hirohata

    International journal of molecular sciences   25 ( 22 )   2024年11月

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    掲載種別：研究論文（学術雑誌）   国際・国内誌：国際誌  

    Osteoarthritis (OA) is a chronic disease affecting over 500 million people worldwide. As the population ages and obesity rates rise, the societal burden of OA is increasing. Pro-inflammatory cytokines, particularly interleukin-1β, are implicated in the pathogenesis of OA. Recent studies suggest that crosstalk between cartilage and synovium contributes to OA development, but the mechanisms remain unclear. Extracellular vesicles (EVs) were purified from cell culture-conditioned medium via ultracentrifugation and confirmed using transmission electron microscopy, nanoparticle tracking analysis, and western blotting. We demonstrated that EVs were taken up by human synoviocytes and chondrocytes in vitro, while in vivo experiments revealed that fluorescent-labelled EVs injected into mouse joints were incorporated into chondrocytes and synoviocytes. EV uptake was significantly inhibited by dynamin-mediated endocytosis inhibitors, indicating that endocytosis plays a major role in this process. Additionally, co-culture experiments with HEK-293 cells expressing red fluorescent protein (RFP)-tagged CD9 and the chondrocytic cell line OUMS-27 confirmed the transfer of RFP-positive EVs across a 600-nm but not a 30-nm filter. These findings suggest that EVs from chondrocytes are released into joint fluid and taken up by cells within the cartilage, potentially facilitating communication between cartilage and synovium. The results underscore the importance of EVs in OA pathophysiology.

    DOI： 10.3390/ijms252211942

    PubMed

  • Manipulation of Macrophage Uptake by Controlling the Aspect Ratio of Graft Copolymer Micelles. 査読

    Yusuke Sakamoto, Shota Fujii, Shin Takano, Jokichi Fukushima, Mitsuru Ando, Noriyuki Kodera, Tomoki Nishimura

    Nano letters   24 ( 19 )   5838 - 5846   2024年05月

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    掲載種別：研究論文（学術雑誌）   国際・国内誌：国際誌  

    Nanostructures of drug carriers play a crucial role in nanomedicine due to their ability to influence drug delivery. There is yet no clear consensus regarding the optimal size and shape (e.g., aspect ratio) of nanoparticles for minimizing macrophage uptake, given the difficulties in controlling the shape and size of nanoparticles while maintaining identical surface properties. Here, we employed graft copolymer self-assembly to prepare polymer micelles with aspect ratios ranging from 1.0 (spherical) to 10.8 (cylindrical) and closely matched interfacial properties. Notably, our findings emphasize that cylindrical micelles with an aspect ratio of 2.4 are the least susceptible to macrophage uptake compared with both their longer counterparts and spherical micelles. This reduced uptake of the short cylindrical micelles results in a 3.3-fold increase in blood circulation time compared with their spherical counterparts. Controlling the aspect ratio of nanoparticles is crucial for improving drug delivery efficacy through better nanoparticle design.

    DOI： 10.1021/acs.nanolett.4c01054

    PubMed

  • A Simple Preparation Method of Gelatin Hydrogels Incorporating Cisplatin for Sustained Release. 査読

    Takahisa Suzuki, Shigeru Tsunoda, Kota Yamashita, Toshie Kuwahara, Mitsuru Ando, Yasuhiko Tabata, Kazutaka Obama

    Pharmaceutics   14 ( 12 )   2022年11月

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    掲載種別：研究論文（学術雑誌）   国際・国内誌：国際誌  

    The objective of this study was to develop a new preparation method for cisplatin (CDDP)-incorporated gelatin hydrogels without using chemical crosslinking nor a vacuum heating instrument for dehydrothermal crosslinking. By simply mixing CDDP and gelatin, CDDP-crosslinked gelatin hydrogels (CCGH) were prepared. CDDP functions as a crosslinking agent of gelatin to form the gelatin hydrogel. Simultaneously, CDDP is incorporated into the gelatin hydrogel as a controlled release carrier. CDDP's in vitro and in vivo anticancer efficacy after incorporation into CCGH was evaluated. In the in vitro system, the CDDP was released gradually due to CCGH degradation with an initial burst release of approximately 16%. CDDP metal-coordinated with the degraded fragment of gelatin was released from CCGH with maintaining the anticancer activity. After intraperitoneal administration of CCGH, CDDP was detected in the blood circulation while its toxicity was low. Following intraperitoneal administration of CCGH in a murine peritoneal dissemination model of human gastric cancer MKN45-Luc cell line, the survival time was significantly prolonged compared with free CDDP solution. It is concluded that CCGH prepared by the CDDP-based crosslinking of gelatin is an excellent sustained release system of CDDP to achieve superior anticancer effects with minimal side effects compared with free CDDP solution.

    DOI： 10.3390/pharmaceutics14122601

    PubMed

  • Single-component nanodiscs via the thermal folding of amphiphilic graft copolymers with the adjusted flexibility of the main chain. 査読

    Tomoki Nishimura, Yusuke Hatatani, Mitsuru Ando, Yoshihiro Sasaki, Kazunari Akiyoshi

    Chemical science   13 ( 18 )   5243 - 5251   2022年05月

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    掲載種別：研究論文（学術雑誌）   国際・国内誌：国際誌  

    Nanodiscs have attracted considerable attention as structural scaffolds for membrane-protein research and as biomaterials in e.g. drug-delivery systems. However, conventional disc-fabrication methods are usually laborious, and disc fabrication via the self-assembly of amphiphiles is difficult. Herein, we report the formation of polymer nanodiscs based on the self-assembly of amphiphilic graft copolymers by adjusting the persistence length of the main chain. Amphiphilic graft copolymers with a series of different main-chain persistence lengths were prepared and these formed, depending on the persistence length, either rods, discs, or vesicles. Notably, polymer nanodiscs were formed upon heating a chilled polymer solution without the need for any additives, and the thus obtained nanodiscs were used to solubilize a membrane protein during cell-free protein synthesis. Given the simplicity of this disc-fabrication method and the ability of these discs to solubilize membrane proteins, this study considerably expands the fundamental and practical scope of graft-copolymer nanodiscs and demonstrates their utility as tools for studying the structure and function of membrane proteins.

    DOI： 10.1039/d2sc01674e

    PubMed

  • Preparation of cationic proteoliposomes using cell-free membrane protein synthesis: the chaperoning effect of cationic liposomes 査読

    Mitsuru Ando, Yoshihiro Sasaki, Kazunari Akiyoshi

    RSC ADVANCES   10 ( 48 )   28741 - 28745   2020年08月（ eISSN:2046-2069 ）

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    担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1039/d0ra05825d

  • Proteoliposome Engineering with Cell-Free Membrane Protein Synthesis: Control of Membrane Protein Sorting into Liposomes by Chaperoning Systems. 査読

    Ando M, Schikula S, Sasaki Y, Akiyoshi K

    Advanced science (Weinheim, Baden-Wurttemberg, Germany)   5 ( 10 )   1800524   2018年10月（ ISSN:2198-3844 ）

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    担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1002/advs.201800524

    PubMed

  • Targeted Delivery of Interferon Gamma Using a Recombinant Fusion Protein of a Fibrin Clot-Binding Peptide With Interferon Gamma for Cancer Gene Therapy 査読

    Mitsuru Ando, Mai Fujimoto, Yuki Takahashi, Makiya Nishikawa, Atsushi Hamana, Yoshinobu Takakura

    JOURNAL OF PHARMACEUTICAL SCIENCES   106 ( 3 )   892 - 897   2017年03月（ ISSN:0022-3549 ） （ eISSN:1520-6017 ）

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    担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1016/j.xphs.2016.11.018

    PubMed

  • Liposome chaperon in cell-free membrane protein synthesis: one-step preparation of KcsA-integrated liposomes and electrophysiological analysis by the planar bilayer method 査読

    M. Ando, M. Akiyama, D. Okuno, M. Hirano, T. Ide, S. Sawada, Y. Sasaki, K. Akiyoshi

    BIOMATERIALS SCIENCE   4 ( 2 )   258 - 264   2016年（ ISSN:2047-4830 ） （ eISSN:2047-4849 ）

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    担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1039/c5bm00285k

    PubMed

  • Comprehensive study of liposome-assisted synthesis of membrane proteins using a reconstituted cell-free translation system 査読

    Tatsuya Niwa, Yoshihiro Sasaki, Eri Uemura, Shugo Nakamura, Minato Akiyama, Mitsuru Ando, Shinichi Sawada, Sada-atu Mukai, Takuya Ueda, Hideki Taguchi, Kazunari Akiyoshi

    SCIENTIFIC REPORTS   5   18025   2015年12月（ ISSN:2045-2322 ）

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    掲載種別：研究論文（学術雑誌）  

    DOI： 10.1038/srep18025

    PubMed

  • Contribution of epigenetic modifications to the decline in transgene expression from plasmid DNA in mouse liver 査読

    Lei Zang, Makiya Nishikawa, Mitsuru Ando, Yuki Takahashi, Yoshinobu Takakura

    Pharmaceutics   7 ( 3 )   199 - 212   2015年08月（ ISSN:1999-4923 ）

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    掲載種別：研究論文（学術雑誌）  

    DOI： 10.3390/pharmaceutics7030199

    PubMed

  • Effects of upregulated indoleamine 2, 3-dioxygenase 1 by interferon gamma gene transfer on interferon gamma-mediated antitumor activity 査読

    K. W Watcharanurak, L. Zang, M. Nishikawa, K. Yoshinaga, Y. Yamamoto, Y. Takahashi, M. Ando, K. Saito, Y. Watanabe, Y. Takakura

    GENE THERAPY   21 ( 9 )   794 - 801   2014年09月（ ISSN:0969-7128 ） （ eISSN:1476-5462 ）

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    掲載種別：研究論文（学術雑誌）  

    DOI： 10.1038/gt.2014.54

  • Enhancement of anticancer effect of interferon-γ gene transfer against interferon-γ-resistant tumor by depletion of tumor-associated macrophages. 査読

    Kiyota T, Takahashi Y, Watcharanurak K, Nishikawa M, Ohara S, Ando M, Watanabe Y, Takakura Y

    Molecular pharmaceutics   11 ( 5 )   1542 - 1549   2014年05月（ ISSN:1543-8384 ）

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    掲載種別：研究論文（学術雑誌）  

    DOI： 10.1021/mp4007216

    PubMed

  • Long-term elimination of hepatitis C virus from human hepatocyte chimeric mice after interferon-γ gene transfer. 査読

    Takahashi Y, Ando M, Nishikawa M, Hiraga N, Imamura M, Chayama K, Takakura Y

    Human gene therapy. Clinical development   25 ( 1 )   28 - 39   2014年03月（ ISSN:2324-8637 ） （ eISSN:2324-8645 ）

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    担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1089/humc.2013.066

    PubMed

  • Prevention of adverse events of interferon γ gene therapy by gene delivery of interferon γ-heparin-binding domain fusion protein in mice. 査読

    Ando M, Takahashi Y, Yamashita T, Fujimoto M, Nishikawa M, Watanabe Y, Takakura Y

    Molecular therapy. Methods & clinical development   1   14023   2014年（ ISSN:2329-0501 ）

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    担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1038/mtm.2014.23

    PubMed

  • Expression Profile-Dependent Improvement of Insulin Sensitivity by Gene Delivery of Interleukin-6 in a Mouse Model of Type II Diabetes 査読

    Hanae Mukumoto, Yuki Takahashi, Mitsuru Ando, Makiya Nishikawa, Yoshinobu Takakura

    MOLECULAR PHARMACEUTICS   10 ( 10 )   3812 - 3821   2013年10月（ ISSN:1543-8384 ） （ eISSN:1543-8392 ）

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    掲載種別：研究論文（学術雑誌）  

    DOI： 10.1021/mp400288e

    PubMed

  • Gene Delivery of Albumin Binding Peptide-Interferon-gamma Fusion Protein with Improved Pharmacokinetic Properties and Sustained Biological Activity 査読

    Noriko Miyakawa, Makiya Nishikawa, Yuki Takahashi, Mitsuru Ando, Masayuki Misaka, Yoshihiko Watanabe, Yoshinobu Takakura

    JOURNAL OF PHARMACEUTICAL SCIENCES   102 ( 9 )   3110 - 3118   2013年09月（ ISSN:0022-3549 ） （ eISSN:1520-6017 ）

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    掲載種別：研究論文（学術雑誌）  

    DOI： 10.1002/jps.23493

    PubMed

  • 遺伝子治療薬・核酸医薬の創製に向けた障壁とその克服戦略 査読

    清水 かほり, 安藤 満

    薬学雑誌   132 ( 12 )   1371 - 1372   2012年12月（ ISSN:0031-6903 ） （ eISSN:1347-5231 ）

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  • [Control of spatiotemporal distribution of interferon γ by genetically fusing functional peptides]. 査読

    Ando M, Takahashi Y, Nishikawa M, Takakura Y

    Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan   132 ( 12 )   1399 - 1406   2012年12月（ ISSN:0031-6903 ） （ eISSN:1347-5231 ）

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  • Constant and steady transgene expression of interferon-γ by optimization of plasmid construct for safe and effective interferon-γ gene therapy. 査読

    Ando M, Takahashi Y, Nishikawa M, Watanabe Y, Takakura Y

    The journal of gene medicine   14 ( 4 )   288 - 295   2012年04月（ ISSN:1099-498X ）

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    担当区分：筆頭著者   掲載種別：研究論文（学術雑誌）  

    DOI： 10.1002/jgm.2616

    PubMed

  • Inhibition of nuclear delivery of plasmid DNA and transcription by interferon γ: hurdles to be overcome for sustained gene therapy 査読

    L. Zang, M. Nishikawa, K. MacHida, M. Ando, Y. Takahashi, Y. Watanabe, Y. Takakura

    Gene Therapy   18 ( 9 )   891 - 897   2011年09月（ ISSN:0969-7128 ）

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    掲載種別：研究論文（学術雑誌）  

    DOI： 10.1038/gt.2011.35

    PubMed

  • Prolonged circulation half-life of interferon γ activity by gene delivery of interferon γ-serum albumin fusion protein in mice. 査読

    Miyakawa N, Nishikawa M, Takahashi Y, Ando M, Misaka M, Watanabe Y, Takakura Y

    Journal of pharmaceutical sciences   100 ( 6 )   2350 - 2357   2011年06月（ ISSN:0022-3549 ）

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    掲載種別：研究論文（学術雑誌）  

    DOI： 10.1002/jps.22473

    PubMed

  • Persistent interferon transgene expression by RNA interference-mediated silencing of interferon receptors 査読

    Yuki Takahashi, Elin Vikman, Makiya Nishikawa, Mitsuru Ando, Yoshihiko Watanabe, Yoshinobu Takakura

    JOURNAL OF GENE MEDICINE   12 ( 9 )   739 - 746   2010年09月（ ISSN:1099-498X ）

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    掲載種別：研究論文（学術雑誌）  

    DOI： 10.1002/jgm.1493

    PubMed

  • Effect of the content of unmethylated CpG dinucleotides in plasmid DNA on the sustainability of transgene expression 査読

    Masaru Mitsui, Makiya Nishikawa, Lei Zang, Mitsuru Ando, Kayoko Hattori, Yuki Takahashi, Yoshihiko Watanabe, Yoshinobu Takakura

    JOURNAL OF GENE MEDICINE   11 ( 5 )   435 - 443   2009年05月（ ISSN:1099-498X ）

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    掲載種別：研究論文（学術雑誌）  

    DOI： 10.1002/jgm.1317

    PubMed

▼全件表示

MISC（その他記事）

  • DDSナノ粒子の表面修飾材料としての細胞膜

    安藤 満

    細胞   58 ( 1 )   72 - 75   2026年01月（ ISSN:1346-7557 ）

    　詳細を見る

    Drug Delivery System(DDS)では,薬剤を必要な部位へ適切に届ける技術が重要である。血球細胞の細胞膜には血中滞留性や標的指向性などDDSに必要な機能が備わっており,膜タンパク質を含む細胞膜をナノ粒子に被覆することで,天然細胞に近い体内動態を付与できる。著者らは内包薬剤を酵素分解依存的に徐放するゼラチンナノ粒子に,血小板や間葉系幹細胞の細胞膜を被覆した細胞膜被覆ゼラチンナノ粒子の開発に取り組んでおり,細胞膜被覆により,膜タンパク質の配向性・機能を保持したままゼラチンナノ粒子の体内動態を改善することに成功している。間葉系幹細胞被覆ゼラチンナノ粒子に薬剤を内包することで,慢性炎症である肝線維症のモデルマウスに対して顕著な治療効果を示した。細胞膜被覆技術は多様なナノ粒子に応用可能であり,炎症制御や再生医療の新たなDDS戦略として期待される。(著者抄録)

  • DDSナノ粒子の表面修飾材料としての細胞膜 招待

    安藤 満

    月刊「細胞」   58 ( 1 )   72 - 75   2026年01月

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    担当区分：筆頭著者,　責任著者   掲載種別：記事・総説・解説・論説等（商業誌、新聞、ウェブメディア）  

  • 膜タンパク質を「材料」として用いる時代は到来するのか？

    安藤 満

    Ｄｒｕｇ Ｄｅｌｉｖｅｒｙ Ｓｙｓｔｅｍ   37 ( 1 )   84 - 85   2022年01月（ ISSN:0913-5006 ） （ eISSN:1881-2732 ）

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    担当区分：筆頭著者  

    DOI： 10.2745/dds.37.84

  • プロテオリポソーム工学:膜タンパク質組込みリポソーム構築のためのシャペロン科学

    安藤満, 吉田昭介, 澤田晋一, 秋吉一成

    膜   39 ( 5 )   2014年（ ISSN:0385-1036 ）

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    担当区分：筆頭著者  

    J-GLOBAL

産業財産権等

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